iScience
·
2024
·
IF 4.6
Ming-Xin Cao,Zhan-Yin Qian,Yan-Jie Liang,Qi-Yao Liu,Han-Ping Wang,Yang Meng,Yin-Song Wang,Yue Wang
Dental caries is a common disease resulting from tooth demineralization caused by bacterial plaque. Probiotics have shown great potential against caries by regulating the balance of oral flora. However, obstacles such as poor colonization and lysozyme sensitivity in oral cavity hinder their further application. In this study, an efficient layer-by-layer surface coating of tannic acid (TA)-Ca 2+ and casein phosphopeptide (CPP) was applied to the probiotic Bifidobacterium infantis (BI) with potential anti-caries activity. Multi-functionalized probiotics thus prepared (called BI@TA-Ca 2+ @CPP) exhibited similar growth pattern, resistance to lysozyme and enhanced colonization potential. The ability of BI@TA-Ca 2+ @CPP in inhibiting cariogenic biofilm formation was improved. Moreover, BI@TA-Ca 2+ @CPP showed excellent remineralization efficacy. In a caries-induced rat model, BI@TA-Ca 2+ @CPP significantly prevented the occurrence and progression of tooth decay, meanwhile showing good biocompatibility. In summary, this study underlines the importance of probiotics coating in antibiofilm and remineralization activity as a promising strategy against dental caries.
研究领域:
龋齿预防
·
口腔微生物学
·
益生菌工程
·
生物矿化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Hui Lv,Shiyu Zong,Tong Li,Qian Zhao,Zhiyong Xu,Jiufang Duan
In recent years, conductive hydrogels have received increasing attention as wearable electronics due to the electrochemical properties of conductive polymers combined with the softness of hydrogels. However, conventional hydrogels are complicated to prepare, require high temperature or UV radiation to trigger monomer polymerization, and are frozen at low temperatures, which seriously hinder the application of flexible wearable devices. In this paper, a conductive sensor integrating mechanical properties, adhesion, UV shielding, anti-dehydration, and anti-freeze was prepared based on Ca2+-initiated radical polymerization at room temperature using the synergy of sodium lignosulfonate, acrylamide (AM), and calcium chloride (CaCl2). Metal ions can activate ammonium persulfate to generate free radicals that allow rapid gelation of AM monomers at room temperature without external stimuli. Due to ionic cross-linking and non-covalent interaction, the hydrogels have good tensile properties (1153% elongation and 168 kPa tensile strength), high toughness (758 KJ·m–3), excellent adhesive properties (48.5 kPa), high ionic conductivity (7.2 mS·cm–1), and UV resistance (94.4%). CaCl2 can inhibit ice nucleation, so that the hydrogels have anti-dehydration and frost resistance properties and even at −80 °C can maintain flexibility, high conductivity, and adhesion. Assembled into a flexible sensor, it can sense various large and small movements such as compression, bending, and talking, which is a flexible sensing material with wide application prospects.
研究领域:
材料科学
·
高分子化学
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可穿戴技术
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2024
·
IF 3.8
Qizheng Dong,Yuanyuan Wang,Xueyou Zhu,Ling He
Ca 2+ and Tm 3+ co-doped CsPbBr 3 @glass composites were successfully synthesized through the high-temperature melting-water quenching-heat treatment method. Enhancement of optical performance is achieved through dual doping, harnessing simultaneous influences from both the glass structure and the inherent structure of CsPbBr 3 quantum dots (QDs). The presence of Ca 2+ in the glass reduces its viscosity and enhances the precipitation of QD crystals. Concurrently, the incorporation of Tm 3+ leads to the control of dimensions and band gaps, reduction in V Br defects, and the potential creation of energy transfer pathways, thereby effectively improving the optical performance of QDs. Simultaneous co-doping with Ca 2+ circumvents the drawback of low crystallinity observed in singly Tm 3+ -doped CsPbBr 3 QDs. In contrast to the undoped sample, the photoluminescence (PL) intensity of the optimized sample demonstrated a significant increase of 128 %. The photoluminescence quantum yield (PLQY) also experienced a rise from 18 % to 55 %, while the fluorescence lifetime extended from 68 ns to 107 ns. Furthermore, the encapsulation of the borosilicate glass matrix has been shown to greatly improve the time, water, thermal, and photostability of CsPbBr 3 QDs. These remarkable properties position the Ca 2+ and Tm 3+ co-doped CsPbBr 3 QDs@glass composites as ideal materials for illumination and wide-color-gamut displays.
研究领域:
材料科学
·
光电技术
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纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Long Wang,Hanyu Gao,Shimin Song,Weiguang Zhou,Na Xue,Yimiao Nie,Bo Feng
The ions activation of quartz is a thorny problem that deteriorates the flotation of metal ore. Polymer inhibitors have exhibited excellent depression performance in the selective flotation of metal-ion activated minerals. In this study, the inhibitory role of sodium alginate (NaAl) in the flotation of Ca 2+ -activated quartz was thoroughly explored by zeta potential, dynamic light scattering (DLS), Fourier transform infrared (FTIR) analysis, X-ray photoelectron spectroscopy (XPS) analysis and adsorption test. The flotation results suggested that NaAl could inhibit the flotation of Ca 2+ -activated quartz in sodium oleate (NaOl) system to different degrees, depending on the addition sequence of NaOl and NaAl. More significant depression of Ca 2+ -activated quartz could be achieved when NaAl was added prior to NaOl. It was found that prominent mutual interaction existed among Ca 2+ , NaOl and NaAl in the bulk solution, probably forming calcium alginate–calcium oleate crosslink product, which was not affected by the addition sequence of these two reagents. However, the interfacial behaviors of these two reagents on Ca 2+ -activated quartz surfaces differed under different reagent conditions. The addition of NaAl after NaOl could not only partially desorb the pre-adsorbed NaOl from mineral surfaces, but also decrease the mineral surface hydrophobicity by polymer coating, which therefore deteriorated the flotation of Ca 2+ -activated quartz. In contrast, when NaAl was added prior to NaOl, the pre-adsorption of NaAl made the further adsorption of NaOl rather difficult, which was likely attributed to the reticulated structure of NaAl and the associated steric hindrance effect. As a result, the flotation of Ca 2+ -activated quartz was tightly depressed.
研究领域:
矿物加工
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化学工程
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材料科学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Haifang Liu,Riting Huang,Xinyu Zhao,Shujuan Yang,Furui He,Wenqi Qin,Junhao Huang,Gaobo Yu,Yuhong Feng,Jiacheng Li,Chenghong Liao
The emulsion gels transformed by Pickering emulsions have great potential as a novel platform for oral drug delivery. In this study, an innovative alginate-based fluorescent colloidal particle was synthesized by Ugi reaction with alginate and carbon dots , which can stabilize Pickering emulsion at the present of Ca 2+ . The Pickering emulsions were converted to emulsion gels during gastric digestion. The microstructure of interfacial film was characterized in situ with confocal laser scanning microscopy. In the process of simulated gastrointestinal digestion, phase conversion of the Pickering emulsion was observed directly in situ by an automatic living cell imaging system. The results showed that clear fluorescent interfacial films can be visualized after simulated oral digestion, and fluorescent spheres were appeared after gastrointestinal digestion, indicating that Pickering emulsions were transformed into emulsion gels. Moreover, results on cell viability and cell uptake showed that the Pickering emulsion had good biocompatibility, which could be taken up by Caco-2 cells and could sustain the release of the drug. The formation of emulsion gels delays the digestion rate and improves the stability of encapsulated curcumin (CUR). In particular, in situ visualization technique also has promising applications in the digestion process and cellular uptake.
研究领域:
药学
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材料科学
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生物医学工程
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Changhao Wu,Xudong Yu,Nan Zhang,Jing Li,Qi Li,Yousheng Yang,Ke Chen,Bo Yang,Shuang Wang,Yiping Liu,Zhenwen Xing
To solve the massive accumulation of gypsum generated from polyhalite leaching and secondary gypsum, this study investigated phase equilibria of the ternary system NH 4 + , Ca 2+ // SO 4 2- - H 2 O at 323.2 K and 348.2 K. The phase diagrams included 2 invariant points, 3 univariate curves, and 3 crystallization regions corresponding to (NH 4 ) 2 SO 4 , CaSO 4 ·2H 2 O, and double salt koktaite (NH 4 ) 2 Ca(SO 4 ) 2 ·H 2 O. As temperature increased, the crystallization region of CaSO 4 ·2H 2 O contracted, whereas those of (NH 4 ) 2 SO 4 and koktaite expanded, indicating that the temperature-enhanced was beneficial to improve the crystallization efficiency of koktaite. Guided by multitemperature phase diagram, the influencing factors and optimal conditions of the comprehensive utilization process of phosphogypsum were explored. The synthesis of koktaite achieved maximum efficiency under conditions of 1: 1.5 molar ratio, 348.2 K reaction temperature, 90 min duration, and 250 rpm agitation. Concurrently, under solid-liquid ratio of 1: 0.5 and after three leaching cycles of 30 seconds each, the yield of CaSO 4 ·2H 2 O production reached 99.80% with a purity of 99.52%. However, the purity and yield of product decreased due to the accumulation of impurities during the cycles, stabilizing after 6 cycles (koktaite yield: 98.50%; purity: 95.69%; (NH 4 ) 2 SO 4 yield: 97.69%; purity: 95.42%). Granulated koktaite released NH 4 + through layer-by-layer dissolution, which prolonged the dissolution time, and the complete dissolution time was 6 times and 24 times longer than that of ungranulated koktaite and (NH 4 ) 2 SO 4 . This work integrates theoretical phase behavior analysis with process engineering to establish a circular resource recovery strategy for synthesizing high-value products from phosphogypsum.
研究领域:
物理化学
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化学工程
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材料科学
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环境科学
·
无机化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 50
·2106106
·
IF 18.808
Jie Shen,Huizhu Yu,Yimeng Shu,Ming Ma,Hangrong Chen
The efficacy of cancer therapy with reactive oxygen species (ROS) as the main therapeutic medium suffers from a deficiency of oxy-substrates, for example, insufficient endogenous hydrogen peroxide (H 2 O 2 ) in chemodynamic therapy (CDT) and inherent hypoxia in photodynamic therapy (PDT). Herein, a smart polyethylene glycol (PEG)-ylated nanosystem CaO 2 @ZIF-Fe/Ce6@PEG (abbreviation as CaZFCP) is constructed to achieve H 2 O 2 /O 2 self-supply and Ca 2+ overloading in tumor cells simultaneously for enhanced CDT/PDT. Under the weakly acidic tumor microenvironment, the activity components inside CaZFCP, that is, CaO 2 nanoparticles, Fe 2+ , and photosensitizer Chlorin e6 (Ce6) are released by the degradation of zeolitic imidazole framework-90 (ZIF-90). Thereinto, CaO 2 nanoparticles are further decomposed to generate H 2 O 2 and O 2 , which alleviates both the insufficient endogenous H 2 O 2 and hypoxia in tumor area, thus enhancing the efficiency of CDT and PDT by producing more hydroxyl radicals and singlet oxygen. Furthermore, Ca 2+ overloading induced by the decomposition of CaO 2 is available for amplifying intracellular oxidative stress, resulting in mitochondrial dysfunction, which further improves the efficacy of combined CDT/PDT. In vitro and in vivo experimental results confirm excellent tumor inhibition effect, which also provides a facile paradigm in ROS-involved cancer therapies.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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药学
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2023
·
IF 10.9
Jianmao Zheng,Jiyuan Zuo,Cairong Xiao,Qining Guo,Xiaobin Fu,Chengyun Ning,Peng Yu
The induction of dental pulp stem cells (DPSCs) into odontogenic differentiation is a cutting-edge method of dental pulp regeneration treatment. However, it remains a challenge to develop biomaterials and therapies that can induce odontogenic differentiation. Here, we propose a wireless electrical stimulation strategy to induce DPSCs odontogenic differentiation via K 0.5 Na 0.5 NbO 3 piezoelectric nanoparticles (KNN) and polarized KNN (PKNN), which can be endocytosed by DPSCs. In vitro, several odontogenic differentiation indexes were also increased in DPSCs treated with KNN and PKNN, and the increase was more obvious in the PKNN group. Intracellular wireless electrical field promoted mitochondrial calcium concentration via mitochondrial calcium unidirectional transporter (MCU), increased the production of adenosine triphosphate (ATP), and induced odontogenic differentiation through the activation of the cAMP-PKA signaling pathway. In vivo, dental pulp-like tissue was induced by electrical stimulation wirelessly with KNN and PKNN, which was more clinically friendly compared with the wired device, and the induction was more obvious in the PKNN group consistent with in vitro experiments. In conclusion, this work demonstrates the potential of PKNN as an effective stimulus that can induce odontogenic differentiation of DPSCs and be applied to dental pulp regeneration treatment.
研究领域:
牙科学
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生物材料
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干细胞生物学
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纳米技术
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chunchun Wang,Jiayi Guo,Qun Liu,Xu Zeng,Yue Liu,Yanping Deng,Yanli Lin,Xialing Wu,Hongju Deng,Linjing Chen,Wuyin Weng,Yucang Zhang
Accidental bleeding is an unavoidable problem in daily life. To avoid the risk of excessive blood loss, it is urgent to design a functional material that can quickly stop bleeding. In this study, an efficient wound dressing for hemostasis was investigated. Based on the characteristics that Ca 2+ and fish skin collagen (FSC) could activate the coagulation mechanism, hemostatic cotton was prepared by solvent replacement method using CaCl 2 , FSC, soluble starch (SS), and polyvinyl alcohol (PVA) as raw materials. The cytotoxicity test showed the Ca 2+ PVA/FSC-SS hemostatic cottons had good biocompatibility. The activated partial thromboplastin time (APTT) of Ca 2+ PVA/FSC-SS(4) was 35.34 s, which was 22.07 s faster than that of PVA/FSC-SS, indicating Ca 2+ PVA/FSC-SS mediated the endogenous coagulation system. In vitro coagulation test, Ca 2+ PVA/FSC-SS(4) could stop bleeding rapidly within 39.60 ± 5.16 s, and the ability of wound healing was higher than commercial product (Celox). This study developed a rapid procoagulant and hemostatic material, which had a promising application in a variety of environments.
研究领域:
生物材料
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止血
·
伤口愈合
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Jiahui Yan,Haoyu Chen,Carlos F. Guimarães,Rui L. Reis,Zhihua Zhu,Tianyi Wang,Fenglan Zhang,Yaqian Lv,Qihui Zhou,Xiaoying Kong,Jinsheng Shi
Ion interference including copper (Cu + )/calcium (Ca 2+ ) overload activate cell-specific death channels, damage mitochondria and disrupt cellular homeostasis, showing great potential in anti-tumor therapy. However, the complex metabolic environment and the powerful self-protection of tumors cause clinical failure of ion interference. Thus, metabolic disruption is expected an innovative strategy for the enhancement of ion interference. Herein, CuS-α-CHCA&penthiopyrad@CaCO 3 -RGD nanoparticles (CCPCR NPs) are prepared to provide a Cu + /Ca 2+ dual-overload anti-tumor therapy assisted by metabolic-symbiosis-destruction strategy, realizing the collapse of tumor self-protection. Specifically, precise CuS and CaCO 3 delivery triggered irreversible Cu + /Ca 2+ dual-overload and reactive oxygen species (ROS) attack toward 4T1 cells. Meanwhile, α-CHCA and penthiopyrad disturbed the metabolic symbiotic environment by disrupting the TCA cycle and preventing lactate efflux to aggravate intracellular acidosis and promote Fenton-like reaction of Cu + , enhancing the sensitivity of tumor cells to copper death and Ca 2+ overload. Further, aided by the metabolism of symbiosis, the destroyed tumor cells further activate the polarization of M1 macrophages and the maturation and antigen cross-presentation of dendritic cells (DCs), which further eliminate tumor cells. In summary, an amplified anti-tumor dual-ion interference strategy assisted by metabolic symbiotic destruction is established, which is of great significance in improving the clinical effect of ion therapy for tumors.
研究领域:
肿瘤学
·
药学
·
免疫学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2022
·Vol. 18
·期 23
·2201123
·
IF 15.153
Jing Li,Yao Zhang,Zhichao Lou,Mingxi Li,Lin Cui,Zhenrong Yang,Lijuan Zhang,Yu Zhang,Ning Gu,Fang Yang
Neural stem cells (NSCs) are used to treat various nervous system diseases because of their self-renewal ability and multidirectional differentiation potential. However, an insufficient ability to track their migration in vivo and poor control over their survival and differentiation efficiency are two major critical challenges for clinical application. Here, it is shown that when magnetic nanobubbles (MNBs), which are assembled from magnetic nanoparticles, are internalized by NSCs, intramembrane volumetric oscillation of the MNBs induces an increase in intracellular hydrostatic pressure and cytoskeleton force, resulting in the activation of the Piezo1-Ca 2+ mechanosensory channel. This subsequently triggers the BMP2/Smad biochemical signaling pathway, leading to differentiation of NSCs into the neuronal phenotype. Signaling through the Piezo1-Ca 2+ -BMP2/Smad pathway can be further accelerated by application of an external shear stress force using low-intensity pulsed ultrasound. More importantly, magnetic resonance imaging and ultrasound imaging surveillance of NSCs based on MNB labeling can be leveraged to provide NSC therapeutic outcomes. Both the in vitro and in vivo findings demonstrate that a bubble nanostructure-induced physical force can modulate and control the mechanical signaling pathway regulating stem cell development.
研究领域:
神经科学
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生物医学工程
·
干细胞生物学
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医学影像学
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力学生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Jipeng Zhang,Xiaojun Zhang,Chongyang Zhu
The self-assembly behavior of proteins and peptides has been widely studied due to their ability to construct various nanostructures, such as nanotubes and nanofibrils. Understanding the self-assembly mechanism is of great significance for developing functional nanomaterials. Herein, we adopt a novel method to construct ovotransferrin (OVT) fibrils by subtilisin A hydrolysis and Ca 2+ ions induced self-assembly with mild reaction conditions. The kinetic process of OVT fibrils formation contains lag, growth, and plateau stage. During the growth stage, OVT peptides self-assemble into precursor structures, which undergo morphological changes of twisted ribbons, helical ribbons, and nanotubes, ultimately closing to form mature fibrils. Ca 2+ ions likely coordinate with the carboxyl groups of OVT peptides, aiding in the self-assembly process. And the secondary conformation is manifested as a β-sheet stacking. The mature OVT fibrils have distinct hollow and left-handed structures. Then OVT fibrils successfully encapsulate zeaxanthin (Zea) and improve its solubility and stability. Besides, OVT fibrils exhibit no in vitro cytotoxicity, and significantly reduce allergenicity to egg allergy patients compared to OVT. OVT fibrils delivery Zea into milk to develop Zea fortified dairy beverage and improve the viscosity of dairy beverage. OVT fibrils show a great potential as nutrient delivery and texture optimization for food application.
研究领域:
食品科学
·
纳米技术
·
生物技术
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）