Journal of Fungi
·
2022
·Vol. 8
·期 12
·1310
·
IF 5.724
Ai-Ai Zhou,Rong-Yu Li,Fei-Xu Mo,Yi Ding,Ruo-Tong Li,Xue Guo,Ke Hu,Ming Li
Background: Natural products are often favored in the study of crop pests and diseases. Previous studies have shown that citronellal has a strong inhibition effect onMagnaporthe oryzae. The objective of this study was to clarify its mechanism of action againstM. oryzae. Results: Firstly, the biological activity of citronellal againstM. oryzaewas determined by direct and indirect methods, and the results show that citronellal had a strong inhibition effect onM. oryzaewith EC50values of 134.00 mg/L and 70.48 μL/L air, respectively. Additionally, a preliminary study on its mechanism of action was studied. After citronellal treatment, electron microscopy revealed that the mycelium became thin and broken; scanning electron microscopy revealed that the mycelium was wrinkled and distorted; and transmission electron microscopy revealed that the mycelium cell wall was invaginated, the mass wall of mycelium was separated, and the organelles were blurred. The mycelium was further stained with CFW, and the nodes were blurred, while the mycelium was almost non-fluorescent after PI staining, and there was no significant difference in the relative conductivity of mycelium. In addition, chitinase was significantly enhanced, and the expression of chitin synthesis-related genes was 17.47-fold upregulated. Finally, we found that the efficacy of citronellal against the rice blast was as high as 82.14% according to indoor efficacy tests. Conclusion: These results indicate that citronellal can affect the synthesis of chitin inM. oryzaeand damage its cell wall, thereby inhibiting the growth of mycelium and effectively protecting rice from rice blasts.Keywords:magnaporthe oryzae;citronellal;cell wall;physiology;cytotoxicity
研究领域:
植物病理学
·
真菌学
·
农药科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·Vol. 14
·期 7
·3269-3278
·
IF 6.1
Ye-Wei Huang,Li-Tian Wang,Meng Zhang,Yan Nie,Jin-Bo Yang,Wen-Luer Meng,Xuan-Jun Wang,Jun Sheng
Increasing low-density lipoprotein receptor (LDLR) protein levels represents a key strategy for the prevention and treatment. Berberine can reportedly alleviate non-alcoholic fatty liver disease (NAFLD) by increasing the LDLR expression in an ERK1/2 signaling-dependent manner of NAFLD. Studies have shown that caffeine can inhibit fat deposition in the livers of mice; however, caffeine has not been reported to alleviate NAFLD by augmenting the LDLR expression via targeting EGFR. Here, an MTT assay, western blotting, RT-qPCR, immunohistochemistry, and surface plasmon resonance (SPR) analysis were used to investigate the role of caffeine in low-density lipoprotein cholesterol (LDL-C) clearance both in vitro and in vivo. In vitro, we found that caffeine could activate the EGFR-ERK1/2 signaling pathway in HepG2 cells, leading to increased LDLR mRNA and protein expression, and this effect could be inhibited by cetuximab. The SPR assay results have indicated that caffeine may increase the LDLR expression by directly binding to the EGFR extracellular domain and activating the EGFR-ERK1/2 signaling pathway. In vivo, caffeine markedly improved fatty liver and related blood indices in ApoE KO mice with high-fat-diet-induced NAFLD. Consistent with our in vitro results, we found that caffeine could also activate EGFR-ERK1/2 signaling and promote the LDLR expression in ApoE KO mice. In summary, caffeine can enhance the LDLR expression by directly binding to EGFR and activating the EGFR-ERK1/2 signaling pathway. EGFR signaling may represent a novel target for the prevention and treatment of NAFLD.
研究领域:
药理学
·
分子生物学
·
细胞生物学
·
病理生理学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jiangshuai Hu,Bin Ji,Rui Wang,Danting Shi,Senlin Shao
A microbe-rich cake layer (i.e., a membrane biofilm) may maintain a stable hydraulic resistance at low fluxes because the microorganisms may decompose organic foulants and create cavities in the cake layer. However, these microorganisms cannot decompose the inorganic particles, which can increase cake resistance because of the synergistic fouling effect with organic foulants. To gain a better understanding of the effect of microorganisms on the microbe-rich cake layer containing inorganic particles, we investigated the change in hydraulic resistance, structure, and composition during the transition of cake layers from a microbe-poor state to a microbe-rich state, at the flux of 5, 10, and 30 L·m −2 ·h −1 . And we used SiO 2 particles (2.5 μm in diameter) and sodium alginate to simulate inorganic particles and organic foulants. The results showed that when the cake layer was transformed from a microbe-poor state to a microbe-rich state, the hydraulic resistance was reduced, the structure became looser, and inorganic particles were partially detached. These changes were more pronounced for the cake layer under an ultra-low flux of 5 L·m −2 ·h −1 , with 62% and 47% reductions in the hydraulic resistance and inorganic particles, respectively. These results indicated that the fouling by inorganic particles could be reduced by the microorganisms at ultra-low fluxes. Cake composition analysis , forces balance analysis, and computational fluid dynamics simulation suggested that the reduced fouling could be derived from the reasons 1) the biodegradation of organic foulants could promote the detachment of inorganic particles, and 2) the microbial-induced cavities at ultra-low fluxes could reduce the synergistic fouling effect by inorganic particles and organic foulants.
研究领域:
环境工程
·
微生物学
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Ke Hu,Rongyu Li,Feixu Mo,Yi Ding,Aiai Zhou,Xue Guo,Ruotong Li,Min Li,Minggui Ou,Ming Li
Dendrobium officinale Kimura et Migo is a traditional Chinese herbal medicinal plant . However, the frequent occurrence of soft rot disease (SRD) is one of the most harmful diseases in D. officinale production in recent years, which can seriously affect its yield and quality. In this study, the major pathogenic fungus (SR-1) was isolated from D. officinale with typical symptoms of SRD, and was identified as Fusarium oxysporum through morphological and molecular identification. The biological activities of five natural products were determined against F. oxysporum using a mycelial growth inhibition assay. The results showed that osthole had the highest antifungal activity against F. oxysporum , with an EC 50 value of 6.40 mg/L. Scanning electron microscopy (SEM) showed that osthole caused F. oxysporum mycelia to shrink and deform. Transmission electron microscopy (TEM) showed that the organelles were blurred and the cell wall was thickened in the presence of osthole. The sensitivity of F. oxysporum to calcofluor white (CFW) staining was significantly enhanced by osthole. Relative conductivity measurements and propidium iodide (PI) observation revealed that osthole had no significant effect on the cell membrane. Further experiments showed that the activity of chitinase and β-1,3-glucanase were decreased, and expression levels of chitinase and β-1,3-glucanase related genes were significantly down-regulated after treatment with osthole. In conclusion, osthole disrupted the cell wall integrity and dynamic balance of F. oxysporum , thereby inhibiting normal mycelial growth.
研究领域:
植物病理学
·
药用植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Han Hu,Caiyun Wu,Fanglan Ge,Yao Ren,Wei Li,Jiao Li
Soybean meal (SBM), a high-protein animal feed, contains several anti-nutritional factors, which limit its utilization. Poly-γ-glutamic acid (γ-PGA), a biological macromolecule , can significantly improve the health and growth benefits of animal feed. However, there are rare reports about SBM fermentation with γ-PGA-producing bacteria to improve its nutritional quality . In this study, we isolated a glutamic acid-independent poly γ-glutamic acid (γ-PGA) producing Bacillus velezensis SCP024–S-4 bacterial strain and used it for solid fermentation of SBM. The results showed that the maximum γ-PGA yield in fermented soybean meal (FSBM) reached up to 89.64 g/kg without adding glutamic acid . Meanwhile, the content of proteins, free amino acids and small peptides increased by 12.83%, 347.96 μg/g and 108.11 mg/g, respectively. Soy globulin, β-conglutinin and trypsin inhibitor all decreased below 3 mg/g. Mice were fed with CK (control diet), SBM and FSBM respectively. The average daily food intake in the FSBM, SBM, and CK groups were 7.68, 5.25, and 6.36 g, respectively. Compared with the CK group, the mice weight growth rate was significantly higher in the FSBM group. Mice blood and spleen tests showed no negative effect of FSBM. The high-throughput sequencing of mice intestinal microflora indicated that FSBM increased the richness of intestinal microbiota ; especially, the types and quantities of Lactobacillus were significantly increased. This study provides a feasible strategy to improve the nutritional quality of SBM as an animal feed.
研究领域:
微生物学
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动物营养
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发酵技术
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食品科学
✓ 已验证
DOI
高影响因子
FEMS MICROBIOLOGY LETTERS
·
2015
·Vol. 362
·期 6
·
IF 2.121
Zhao Jianqing,Pan Shan,Lin Lan,Fu Lei,Yang Chao,Xu Zhongmin,Wei YanMin,Jin Meilin,Zhang Anding
Streptococcus suis (S. suis) ranks among the five most important porcine pathogens worldwide and occasionally threatens human health, especially in people that come into close contact with pigs or pork products. Streptococcus suis serotype 2 (SS2) is considered to be the most pathogenic and prevalent capsular type. As a first line of immune defense against SS2 infection, neutrophils can eliminate the invader not only by phagocytosis but also by neutrophil extracellular traps (NETs)-mediated killing. SS2 can resist phagocytosis through polysaccharide capsule (CPS), but how this strain evades the effects of NETs remains to be determined. The present study demonstrated that the epidemic strain 05ZY, the highly pathogenic strain P1/7 and the intermediately pathogenic strain A7 could induce the formation of NETs. Furthermore, SS2 strains could successfully resist NETs-mediated killing, and the CPS structure contributed to this resistance by escaping the trapping. Therefore, the CPS structure not only contributed to the SS2 strains’ resistance to phagocytosis-mediated killing but also played an essential role in evading NETs trapping and further killing in vitro. This study strengthens our understanding of how S. suis can evade innate immune surveillance and elimination.
研究领域:
微生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Environmental Science & Engineering
·
2023
·Vol. 18
·期 2
·1-11
·
IF 6.4
Liu Yiwei,Gu Kaili,Zhang Jinhua,Li Jinxiang,Qian Jieshu,Shen Jinyou,Guan Xiaohong
Sulfated zero-valent iron (SZVI) has shown promising applications in wastewater treatment. However, the rapid decline in the reactivity of SZVI with time limits its real practice. To mediate this problem, partial aging was proposed to improve the reactive durability of SZVI. Taking Cr(VI) as the target contaminant, we found that the aged ZVI (AZVI) gradually lost reactivity as aging time increased from 0.5 to 2 d. Counter-intuitively, the partially aged SZVI (ASZVI) showed greater reactivity than SZVI when exposed to oxygenated water for a period ranging from 0.5 to 14 d. In addition, the ASZVI with 0.5 d of aging time (ASZVI-0.5) not only maintained reactivity in successive runs but also increased the Cr(VI) removal capacity from 9.1 mg/g by SZVI to 19.1 mg/g by ASZVI-0.5. Correlation analysis further revealed that the electron transfer from the Fe 0 core to the shell was mediated by the conductive FeS and FeS 2 in the subshell of ASZVI. Meanwhile, the lepidocrocite and magnetite on the surface of ASZVI facilitated Cr(VI) adsorption and subsequent electron transfer for Cr(VI) reduction. Moreover, the iron (hydr)oxide shell could retain the conductive FeS and FeS 2 in the subshell, allowing ASZVI to reduce Cr(VI) efficiently and sustainably. In general, partial aging can enhance the reactive durability of ZVI when coupled with sulfidation and this synergistic effect will be beneficial to the application of SZVI-based technology for wastewater treatment.
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理技术
✓ 已验证
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2020
·
IF 4.3
Jinpeng Wang,Ming Zhang,Runjuan Zhou,Jiyuan Li,Wei Zhao,Wenyuan Chen
This paper explored the effect of trace Cu(Ⅱ) (5 μM) on the degradation of Orange Ⅱ by Fe(Ⅱ)/Hydroxylamine (HA)/Persulfate (PS) system. The results showed that the addition of trace Cu(Ⅱ) not only improved the degradation efficiency of Fe(Ⅱ)/HA/PS system, but also broadened the pH range of Fe(Ⅱ)/HA/PS degradation Orange Ⅱ (2–10). Increasing the dosage of Fe(Ⅱ), Cu(Ⅱ) and PS will promote the degradation of Orange Ⅱ by the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. However, the optimal dosage of HA is 0.03 mM. Lower or excessive HA dosage is not conducive to the degradation of Orange Ⅱ by the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. Scavenger experiments show that SO 4 ·- is the main reactive oxygen species (ROS) in the Cu(Ⅱ)/Fe(Ⅱ)/HA/PS system. In both acidic and alkaline environments, Cu(Ⅰ) reduced by hydroxylamine can promote the conversion of Fe(Ⅲ) to Fe(Ⅱ). In acidic environment, Fe(Ⅱ) reduced by Cu(Ⅰ) is the reason why trace Cu(Ⅱ) can enhance the efficiency of Fe(Ⅱ)/HA/PS system to degrade Orange Ⅱ. However, in alkaline environment, Cu(I) reduced by hydroxylamine is the main participant in the production of SO 4 ·- by activating persulfate, which is also the main reason for broadening the applicable pH of Fe(Ⅱ)/HA/PS for Orange Ⅱ degradation to alkaline environment.
研究领域:
环境科学
·
化学
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Lingyu He,Xin Zhang,Yang Lei
This study proposes a hybrid electrochemical oxidation-coagulation (EOC) system that integrates a Ti₄O₇ anode with a cathode fabricated from waste aluminum cans, enabling the simultaneous degradation of phosphonates and removal of hardness ions from cooling water. Compared to standalone electrooxidation (EO) and conventional chemical coagulation (CC), the EOC system achieved 41.9% Ca²⁺ removal, 91% total soluble phosphorus (TSP) elimination, and 67.2% conversion of phosphonate to phosphate within 2 hours. Mechanistic studies revealed that the hybrid EOC system performs a synergistic effect, including anodic oxidation of antiscalant (nitrilotris methylene phosphonic acid, NTMP), and the cathodic dissolution of aluminum, generating coagulants that facilitate the concurrent removal of hardness ions and residual phosphorus. The presence of chloride ions enhanced NTMP degradation via the generation of reactive chlorine species (RCS) without impacting Ca²⁺ removal efficiency, whereas the presence of magnesium ions promoted Ca²⁺ removal via Mg(OH)₂ formation, which provides nucleation sites for co-precipitation. On top of mechanical insights, practical validation with real cooling water confirmed the practical effectiveness and robustness of the EOC system. Moreover, energy consumption analysis revealed that the EOC system significantly reduced energy demand for calcium and TSP removal by 91% and 76%, respectively, compared to the EO system. Overall, this work underscores an efficient and cost-effective electrochemical approach for industrial cooling water treatment, establishing foundational insights for scalable implementation.
研究领域:
环境工程
·
电化学
·
水处理技术
·
可持续材料回收
·
工业废水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY AND INDUSTRIAL HEALTH
·
2024
·
IF 1.7
Shuang Chen,Chengzhi Liu,Yifan Yang,Jiangliang Chu,Beilei Yuan,Zhe Wang
Silica nanoparticles (SiNPs) are widely utilized in occupational settings where they can cause lung damage through inhalation. The objective of this research was to explore the metabolic markers of SiNPs-induced toxicity on A549 cells by metabolomics and provide a foundation for studying nanoparticle-induced lung toxicity. Metabolomics analysis was employed to analyze the metabolites of SiNPs-treated A549 cells. LASSO regression was applied for selection, and protective measure experiments were conducted to validate the efficacy of selected potential toxicity mitigators. After SiNPs treatment, 23 differential metabolites were identified, including lipids, nucleotides, and organic oxidants. Pathway analysis revealed involvement in various biological processes. LASSO regression further identified six metabolites significantly associated with SiNPs toxicity. Notably, phosphatidylethanolamine (PE (14:1(9Z)/14:0)) showed enrichment in six significant metabolic pathways and with an AUC of 1 in the ROC curve. Protective measure experiments verified its protective effect on A549 cells and demonstrated its considerable inhibition of SiNPs-induced cytotoxicity. This study elucidated SiNPs-induced cytotoxicity on A549 cells and identified PE as a potential toxicity mitigator. These findings contribute to understanding the mechanisms of nanoparticle-induced lung toxicity and inform occupational health preventive strategies.
研究领域:
毒理学
·
生物化学
·
职业健康
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 5
·6269-6282
·
IF 2.478
Chen Zhangxu,Fu Minglian,Zheng Bingyun,Zhu Danchen,Chen Zefan
The influencing factors of chelator, reaction times, and Yb 3+ /Tm 3+ -doped concentration on the formation of NaYF 4 :Yb,Tm crystals with various morphologies have been investigated and determined. It is found that NaYF 4 :Yb,Tm with various morphologies and different phases can be tuned. Compared with the addition of ethylene diamine tetra acetic acid, hexagonal nanoprism-like β-NaYF 4 :Yb,Tm with a mean length of ~ 1300 nm and diameter of ~ 100 nm is prepared in the presence of oleic acid, which preferentially orients along with the [0001] direction more quickly than others. Systematical research on the emission of Yb 3+ /Tm 3+ -doped NaYF 4 crystals with microrod, hexagonal microprism, and nanoplate has implied that these obtained crystals with unique optical properties are closely related to their morphologies, phases, and sizes. In addition, a possible growth mechanism of co-existence of α- and β-NaYF 4 phases is proposed. It can be expected to illustrate the formation mechanism for the upconversion crystals or other rare-earth upconversion materials and to import these highly luminescent microcrystals or nanocrystals to environmental remediation by full solar energy spectra.
研究领域:
材料科学
·
纳米技术
·
光学材料
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
FERROELECTRICS
·
2024
·
IF 0.6
Chenghong Huang,Xiaorong Huang,Qi Yin,Xuehan Hu,Ying Fang,Hongjie Chen,Lin Zhang,Kang Yang,Hao Li,Fang Yang,Xiaoling Lu,Zeng Yang
Chitosan (CS) is lack of applications in pharmaceutical industry due to its lower compression density and instability during tablet process. This study mainly focus on the synthesis of ethyl vanillin incorporated CS(EV/CS) via Schiff base reaction and the evaluation of application as pharmaceutic excipient. Results of FT-IR showed that there is a typical amide peak in EV/CS that the 1640 cm−1 can be attributed to –C = N– bond and 1518 cm−1 can be attributed to backbone of benzene but not in CS raw material. Compared with the control, XRD of EV/CS have additional 2θ angle of 44°, 64° and 77° diffraction peaks except that the 2θ = 20° of EV is still existed. More slower weight loss of EV/CS than that of CS in the range of 248–760 °C is also demonstrated by TGA analysis. 1H NMR indicated there are intrinsic 7.5 and 7.1 ppm as well as emerging 4.2 ppm of chemical shift in EV/CS. The physical finger spectra from twelve indexes of EV/CS powder denotes quite differences in compressibility, fluidity and stability with that of CS. 5% content EV/CS of designed formulation can at most decrease the friability and disintegration time of the compressed tablet. In conclusion, the developed EV/CS is inclined to suitable for tablet as an excipient.
研究领域:
药学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）