ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Ying-Xue Liang,Yi-Ke Wang,Wei-Jie Meng,Qian Wang,Jia-Xin Li,Wei-Hua Huang,Min Xie
Traditional methods for the detection of pathogenic bacteria are time-consuming, less efficient, and sensitive, which affects infection control and bungles illness. Therefore, developing a method to remedy these problems is very important in the clinic to diagnose the pathogenic diseases and guide the rational use of antibiotics. Here, microfluidic electrochemical integrated sensor (MEIS) has been investigated, functionally for rapid, efficient separation and sensitive detection of pathogenic bacteria. Three-dimensional macroporous PDMS and Au nanotube-based electrode are successfully assembled into the modeling microchip, playing the functions of “3D chaotic flow separator” and “electrochemical detector,” respectively. The 3D chaotic flow separator enhances the turbulence of the fluid, achieving an excellent bacteria capture efficiency. Meanwhile, the electrochemical detector provides a quantitative signal through enzyme-linked immunoelectrochemistry with improved sensitivity. The microfluidic electrochemical integrated sensor could successfully isolate Candida albicans (C. albicans) in the range of 30–3,000,000 CFU in the saliva matrix with over 95% capture efficiency and sensitively detect C. albicans in 1 h in oral saliva samples. The integrated device demonstrates great potential in the diagnosis of oral candidiasis and is also applicable in the detection of other pathogenic bacteria.
研究领域:
医学诊断
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微生物学
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生物传感器技术
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PubMed
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2024
·Vol. 28
·期 9
·e18354
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IF 4.3
Longfei Yang,Xiaonan Wang,Zhiming Ma,Yujie Sui,Xin Liu
Infections caused by Candida species, especially Candida albicans , threaten the public health and create economic burden. Shortage of antifungals and emergence of drug resistance call for new antifungal therapies while natural products were attractive sources for developing new drugs. In our study, fangchinoline, a bis-benzylisoquinoline alkaloid from Chinese herb Stephania tetrandra S. Moore, exerted antifungal effects on planktonic growth of several Candida species including C. albicans , with MIC no more than 50 μg/mL. In addition, results from microscopic, MTT and XTT reduction assays showed that fangchinoline had inhibitory activities against the multiple virulence factors of C. albicans , such as adhesion, hyphal growth and biofilm formation. Furthermore, this compound could also suppress the metabolic activity of preformed C. albicans biofilms. PI staining, followed by confocal laser scanning microscope (CLSM) analysis showed that fangchinoline can elevate permeability of cell membrane. DCFH-DA staining suggested its anti- Candida mechanism also involved overproduction of intracellular ROS, which was further confirmed by N-acetyl-cysteine rescue tests. Moreover, fangchinoline showed synergy with three antifungal drugs (amphotericin B, fluconazole and caspofungin), further indicating its potential use in treating C. albicans infections. Therefore, these results indicated that fangchinoline could be a potential candidate for developing anti- Candida therapies.
研究领域:
微生物学
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药理学
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真菌学
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天然产物
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抗菌剂
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
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IF 2.8
Zhen-Yu Zhao,Ya-Ning Che,Qian Huang,Han-Wen Yang,Xian-Hua Wang,Lin-Yi Dong
Candida albicans is the leading cause of invasive fungal disease; candidemia carries 30–40 % mortality. It commonly colonizes human mucosa as a commensal, but immunosuppression or disrupted microbiota enables opportunistic infection. Therefore, rapid, reliable diagnostics are vital to improve public health. In this study, a magnetic solid-phase microextraction (MSPE) technique based on nystatin-functionalized magnetic nanoparticles (Fe₃O₄@NYS) was developed to achieve the dual objectives of efficiently enriching C. albicans rapidly and extracting its deoxyribonucleic acid (DNA). Optimization of capture conditions enabled rapid enrichment of C. albicans with a maximum recovery rate of approximately 90 %. Subsequent to the capture of C. albicans, DNA extraction was performed on the Fe₃O₄@NYS- C. albicans complexes. Notably, Fe₃O₄@NYS facilitated the extraction of a substantial quantity of DNA, with the extraction yield reaching 1200 ng/mg. Therefore, the integration of DNA extraction with quantitative polymerase chain reaction (qPCR) technology enables sensitive and specific detection of C. albicans in real samples. The strategy demonstrated excellent linearity within the concentration range of 4.20 × 10 2 to 4.20 × 10 7 CFU mL −1 for C. albicans . The high sensitivity and accuracy of this method were confirmed in honey samples, showcasing a limit of detection (LOD) 420 CFU mL −1 , recoveries between 98.1 % and 99.8 %, and relative standard deviations (RSD) not exceeding 2.51 %. This method presents an innovative strategy for point-of-care clinical diagnosis, disease surveillance, and biosafety management, demonstrating substantial potential for the efficient isolation of DNA from complex biological matrices.
研究领域:
微生物学
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临床诊断
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纳米技术
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分子生物学
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传染病
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PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·Vol. 127
·期 6
·4251-4255
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IF 1.289
Wentao Liu,Hongdong Duan,Xia Meng,Dawei Qin,Xingjian Wang,Juan Zhang
A series of macroporous beaded terpolymers with epoxy groups were synthesized by suspension polymerization with glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA), and the third monomers including styrene, methyl methacrylate, n -butyl acrylate (BA), butyl methacrylate (BMA), and 2-hydroxyethyl methacrylate for immobilization of Candida lipolytica lipase. The effect of various third monomers on loading and activity recovery of immobilized lipase were studied. Terpolymers with BA as the third monomer were found to give the biggest loading of lipase, and the activity recovery of lipase immobilized on poly(GMA-EGDMA-BA) terpolymers reached 79.0%. As the content of BA (%) increasing, the loading of lipase enhanced, but the activity recovery reached 88.5% for the initial stage and decreased to 46.9% at last. The poly(GMA-EGDMA-BA-10) showed an optimal result in lipase immobilization. Lipase immobilized on poly(GMA-EGDMA-BA-10) carriers had broader pH and higher temperature stability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
研究领域:
生物化学
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高分子科学
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酶工程
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生物催化
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材料化学
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DOI
高影响因子
Annals of Translational Medicine
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2020
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IF 3.297
Ying Li,Mingzhu Shan,Shihui Li,Yuechen Wang,Huan Yang,Ying Chen,Bing Gu,Zuobin Zhu
Background Candidiasis has long been a threat to human health, but cytotoxicity and resistance always block the usefulness of antifungal agents. The ability to switch between yeast and hypha is one of the most discussed virulence trait attributes of the human pathogenic fungus Candida albicans . The morphological transition provides a novel target for developing antifungal drugs. The aim of the present study was to explore the activity and mechanism of teasaponin (TS), a generally regarded as safe natural product, in inhibiting filamentation of C. albicans , hoping to provide an experimental basis for its clinical application. Methods The effect of TS on filamentation and biofilm formation of C. albicans was evaluated by XTT reduction assay and microscopy. The level of intracellular cAMP was measured to further explore the underlying mechanism. In addition, cytotoxicity of TS was evaluated by using MTT assay in vitro and Caenorhabditis elegans model in vivo . The potential of TS-resistance induction was tested by a serial passage experiment. Results TS displayed a moderate antifungal activity against the wild type, efflux pump mutant and multi-resistance C. albicans strains, and could effectively retard filamentation and biofilm formation with a low MIC value. Further mechanism investigation revealed that the reduced cAMP level inhibited filamentation and biofilm formation. In addition, TS showed no significant cytotoxicity in vitro or in vivo , and had little potential to develop resistance during long-time induction. Conclusions Our work evaluated the antifungal activity of TS against filamentation and biofilms formation of C. albicans and disclosed the underlying mechanism, which might provide useful clues for the potential clinical application of TS in fighting clinical fungal infections by targeting the virulence factors.
研究领域:
微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Na Tang,Shenghao Yuan,Yuxuan Luo,An-Jun Wang,Kang Sun,Ning-Ning Liu,Ke Tao
Biofilm formation is a critical event in the pathogenesis and virulence of fungal infections caused by Candida albicans, giving rise to about a 1000-fold increase in the resistance to antifungal agents. Although photodynamic treatment (PDT) has been excellently implicated in bacterial infections, studies on its potential against fungal infection through the clearance of fungal biofilm formation remain at its infancy stage. Here, we have designed photodynamic nanoparticles with different sizes, modifications, and the ability of generating reactive oxygen species (ROS) to examine their effects on inhibiting biofilm formation and destructing mature biofilms of C. albicans. We found that the nanoparticles modified with oligo-chitosan exhibited a better binding efficiency for planktonic cells, leading to stronger inhibitory efficacy of the filamentation and the early-stage biofilm formation. However, for mature biofilms, the nanoparticles with the smallest size (∼15 nm) showed the fastest penetration speed and a pronounced destructing effect albeit conferring the lowest ROS-producing capability. The inhibitory effect of photodynamic nanoparticles was dependent on the disruption of fungal quorum sensing (QS) by the upregulation of QS molecules, farnesol and tyrosol, mediated through the upregulation of ARO 8 and DPP 3 expression. Our findings provide a powerful strategy of nanoparticulate PDT to combat fungal infections through the inhibition of both hyphal and biofilm formation by disrupting QS.
研究领域:
微生物学
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医学
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药理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 11
·9350
·
IF 5.6
Yuhong Chen,Ying Gao,Mingan Yuan,Zhaisheng Zheng,Junfeng Yin
Candida albicansis an opportunistic human fungal pathogen, and its drug resistance is becoming a serious problem.Camellia sinensisseed saponins showed inhibitory effects on resistantCandida albicansstrains, but the active components and mechanisms are unclear. In this study, the effects and mechanisms of twoCamellia sinensisseed saponin monomers, theasaponin E1 (TE1) and assamsaponin A (ASA), on a resistantCandida albicansstrain (ATCC 10231) were explored. The minimum inhibitory concentration and minimum fungicidal concentration of TE1 and ASA were equivalent. The time–kill curves showed that the fungicidal efficiency of ASA was higher than that of TE1. TE1 and ASA significantly increased the cell membrane permeability and disrupted the cell membrane integrity ofC. albicanscells, probably by interacting with membrane-bound sterols. Moreover, TE1 and ASA induced the accumulation of intracellular ROS and decreased the mitochondrial membrane potential. Transcriptome and qRT-PCR analyses revealed that the differentially expressed genes were concentrated in the cell wall, plasma membrane, glycolysis, and ergosterol synthesis pathways. In conclusion, the antifungal mechanisms of TE1 and ASA included the interference with the biosynthesis of ergosterol in fungal cell membranes, damage to the mitochondria, and the regulation of energy metabolism and lipid metabolism. Tea seed saponins have the potential to be novel anti-Candida albicansagents.Keywords:Camellia sinensisseeds;theasaponin E1;assamsaponin A;antifungal activity;transcriptome
研究领域:
药理学
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微生物学
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分子生物学
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植物科学
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PubMed
高影响因子
Molecular Catalysis
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2022
·
IF 5.089
Bin Zou,Feng Liu,Yan Yan,Ting Feng,Josephine Naa Norkor Dowuona
In this study, coupling with substrate ultrasonic pretreatment technology, magnetic cross-linked enzyme aggregates (CRL-FIL-CLEAs@Fe 3 O 4 ) was used as novel biocatalyst for efficient preparation of phytosterol oleate in solvent-free reaction system. The CRL loading was 0.16 mg protein/mg CRL-FIL- CLEAs@Fe 3 O 4 . The effects of ultrasonic parameters, catalyst dosage, substrate molar ratio, and reaction temperature on the synthesis of phytosterol esters were studied. The results showed that the catalytical properties of CRL-FIL-CLEAs@Fe 3 O 4 (7.09±0.11 µmol phytosterol/mg protein. hour) was significantly improved compared with natural lipase from Candida rugosa (CRL, 5.13±0.21 µmol phytosterol/mg protein. hour). Simultaneously, ultrasonic pretreatment greatly enhanced substrate mass transfer, further reducing the esterification time. The max conversion rate 94.72% of phytosterol was obtained after 9 h reaction under optimum condition (the molar ratio of oleic acid to phytosterol was 6:1, the concentration of CRL-FIL-CLEAs@Fe 3 O 4 was 10 wt% of the phytosterol mass). After five recycles, the activity of CRL-FIL-CLEAs@Fe 3 O 4 (80.02%) was still 2.68 times higher than that of natural lipase system (29.91%). Meanwhile, the novel immobilized lipase (CRL-FIL-CLEAs@Fe 3 O 4 ) before and after reaction was characterized by SEM, AFM, VSM, TEM, and TGA. The results showed that CRL-FIL-CLEAs@Fe 3 O 4 was a robust and recyclable biocatalyst for phytosterol esterification.
研究领域:
生物催化
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酶工程
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食品科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Mingming Zheng,Shi Wang,Xia Xiang,Jie Shi,Juan Huang,Qianchun Deng,Fenghong Huang,Junyong Xiao
In this study, Candida lipolytica lipase ( CLL ) was immobilized on magnetic multi-walled carbon nanotubes (mMWCNTs) via hydrophobic and cation-exchange interaction. The resultant immobilized CLL showed much better thermal stability, biocatalyst activity and easier recycling than the free form. A method for efficient enzymatic acidolysis of tripalmitin (PPP) with oleic acid (OA), to produce OPO-rich TAGs, was developed, using the immobilized CLL as the biocatalyst. Under optimized conditions (2% water, 20 mg/ml enzyme, 1:6 PPP/OA, 50 °C, 2 h), the content of OPO in the final product reached 46.5%. CLL @mMWCNTs had a better activity and manipulative stability than commercial lipases. More importantly, the feasibility of CLL @mMWCNTs was also validated in the practical production of OPO-rich TAGs, using lard and restructured palm oil as the raw material. These results suggest that CLL @mMWCNTs is a promising biocatalyst for the OPO-rich TAGs production and will be helpful for the infant formula industry.
研究领域:
生物催化
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食品科学
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酶工程
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DOI
PubMed
高影响因子
BIOFOULING
·
2026
·
IF 2.5
Xue Wang,Chengxi Li
Candida albicans (C. albicans) biofilms exhibit markedly enhanced tolerance to conventional antifungal agents, necessitating the identification of novel therapeutic alternatives. Chrysin, a naturally occurring flavonoid distributed in propolis and Passiflora species, possesses well-documented antioxidant and anti-inflammatory properties. However, its activity against C. albicans biofilms remains uncharacterized. This study comprehensively evaluated the antibiofilm efficacy of chrysin in vitro and in vivo. The sessile minimum inhibitory concentration causing 50% inhibition (SMIC50) of chrysin against C. albicans SC5314 was 128 μg/mL. Chrysin suppressed biofilm metabolic activity, reduced biomass, and decreased cell viability in a concentration-dependent manner, as demonstrated by XTT reduction assay, crystal violet staining, and live/dead fluorescence staining. Scanning electron microscopy (SEM) revealed progressive dismantling of hyphal networks and disruption of biofilm architecture. Chrysin significantly elevated intracellular reactive oxygen species (ROS) levels and induced mitochondrial membrane potential (MMP) depolarization in a dose-dependent manner, indicating that oxidative stress induction is a key antifungal mechanism. In a murine oral candidiasis model, chrysin reduced fungal burden and tongue lesion scores in a concentration-dependent manner, with the 256 μg/mL group achieving outcomes comparable to fluconazole. Chrysin also attenuated pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), while increasing interleukin-10 (IL-10). Biosafety assessment demonstrated cell viability above 80% in human oral keratinocytes (HOK) and above 85% in mouse fibroblasts (L929) at concentrations up to 512 μg/mL, with no observable organ toxicity in vivo. Collectively, these findings establish chrysin as a potent, mechanistically defined, and safe natural antifungal candidate with promising translational potential for the management of C. albicans biofilm-associated infections.
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PubMed
高影响因子
CLINICAL ORAL IMPLANTS RESEARCH
·
2014
·Vol. 26
·期 3
·332-341
·
IF 3.123
Nicolas Villard,Chaminda Seneviratne,James Kit Hon Tsoi,Markku Heinonen,Jukka Matinlinna
Objectives The aim of the present study was to evaluate the effect of novel silane system coatings on zirconia and titanium implant surfaces and the attachment of the fungal pathogen Candida albicans . Materials and methods Titanium and zirconia specimens were silica-coated and silanized either with a commercial silane primer (RelyX Ceramic Primer™, 3M ESPE) or a novel silane system primer. The novel silane system primer was a blend of 1.0 vol% 3-acryloxypropyltrimethoxysilane and 0.3 vol% bis-1,2-(triethoxysilyl)ethane diluted in acidified ethanol-water solvent. The surface roughness (R a ), the surface free energy and the chemical composition of substrate surfaces after treatments were evaluated. C. albcans biofilms were developed on silica-coated + silanized surfaces during 48 h of incubation time. Colony forming units (CFU) and real-time PCR (RT-PCR) quantified the cells on the material surfaces. Statistical analyses were carried out by 1-way ANOVA, Tukey post hoc and Games Howell post hoc test at 5% significance level (p) . Results On zirconia and titanium surfaces, the R a and the chemical composition of the specimens were equal ( P < 0.05). The surface free energy was decreased on titanium specimens and increased on zirconia specimen after silanization. CFU of C. albicans was significantly lower on zirconia coated with RelyX Ceramic Primer™, ( P < 0.001) and on titanium coated with both silanes ( P = 0.002). RT-PCR revealed no differences between the mean quantities of C. albicans ( P ≥ 0.067). Conclusion Silica-coating and silanization had modified the titanium and zirconia surfaces significantly. Both the control and experimental silane primers might inhibit the biofilm formation of C. albicans .
研究领域:
口腔医学
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微生物学
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材料科学
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DOI
PubMed
高影响因子
RESEARCH IN MICROBIOLOGY
·
2025
·
IF 3.4
Ziran Wang,Yuanyuan Lu
Candida albicans , a prevalent opportunistic fungal pathogen, employs dimorphic transition (yeast-to-hypha) as a central strategy for host tissue invasion and immune evasion. Although prior studies have linked ADH1 deletion to attenuated virulence phenotypes such as impaired hyphal formation, the molecular mechanism underlying this phenomenon remains elusive. Here, we report that ADH1 knockout strains exhibit a striking hyperelongation of hyphae, deviating from the characteristic branched architecture observed in wild-type strains. Transcriptomic profiling identified arginine metabolism as the most significantly activated pathway in adh1Δ/Δ mutants, with marked upregulation of CAR1 , encoding a key arginase. Crucially, pharmacological or genetic inhibition of Car1 activity fully restores wild-type hyphal morphology in ADH1 knockout strains, unequivocally establishing that ADH1 governs hyphal development through repression of CAR1 expression. Our findings delineate the Adh1-Car1 metabolic axis as a master regulator of dimorphic switching in C. albicans : while Adh1 constrains Car1 to maintain balanced hyphal branching, its deletion triggers arginine metabolic flux dyshomeostasis, driving uncontrolled hyphal hyperelongation. This work redefines the functional paradigm of Adh1 beyond its canonical role in ethanol metabolism, positions fungal metabolic rewiring as a direct driver of morphogenic plasticity, and nominates the Adh1-Car1 axis as a high-value target for antifungal interventions.
研究领域:
微生物学
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分子生物学
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真菌致病机制
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代谢调控
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遗传学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）