Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122219
·
IF 7.5
Qian Zhang,Fu Wei,Shumin Qin,Bixiang Nie,Lin Deng,Zheng Chen,Siyu Hua
Heterotrophic nitrifying-aerobic denitrifying (HN-AD) bacteria- Chlorella symbiotic systems face several limitations, including high energy consumption, weak symbiosis and low pollutant removal efficiency. To address these issues, this study optimized the aeration rate. In addition, three quorum sensing (QS) molecules, namely C4-HSL, C6-HSL and C8-HSL, were introduced based on the structural chain length gradient of signaling molecules. This approach aimed to evaluate their effects on the system's macroscopic performance and to explore the underlying enhancement mechanisms. The results showed that under the optimal aeration rate of 0.6 L·min −1 , C8-HSL significantly enhanced pollutant removal, increasing the average removal efficiencies of NH₄⁺-N, TN, TP and COD by 9.09%, 10.81%, 13.78% and 14.25%, respectively. Highthroughput sequencing revealed that C8-HSL markedly enriched Corynebacterium (48.72%), strengthening bacterial-algal symbiosis and improving nitrogen and phosphorus removal. PICRUSt2 functional prediction indicated that C8-HSL enhanced nitrogen metabolic flux by upregulating key genes involved in denitrification and nitrogen fixation ( nirK and nifDHK ), while also significantly increasing the abundance of the phosphorus metabolism related pat gene. qPCR analysis further confirmed the elevated expression of nirK and nifDHK , validating the reliability of PICRUSt2 predictions. These findings demonstrate that C8-HSL effectively improves the performance of the HN-AD bacteria- Chlorella symbiotic system and provides a theoretical basis for the engineering application of QS molecules.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Ruohua Qu,Qiting Xie,Jiang Tian,Min Zhou,Fei Ge
Phosphorus removal by algae-based biotechnology can be achieved through algal assimilation, surface adsorption, or abiotic precipitation. However, there are still unavailable how these phosphorus removal processes were affected by nanoparticles in wastewater. Here, we employed a non-targeted metabolomic approach to reveal the impact of silver nanoparticles (AgNPs) on the phosphorus assimilation by a unicellular green alga Chlorella vulgaris F1068 ( C. vulgaris F1068). Results showed that AgNPs mostly inhibited total phosphorus (TP) removal by the algal assimilation, with TP removal efficiency being reduced by 66.2% (with 0.20 mg/L AgNPs) of the control (without AgNPs). Metabolomics analysis also indicated that AgNPs disturbed metabolic responses related to phosphorus assimilation. AgNPs inhibited phospholipid metabolism which included inositol phosphate metabolism and phosphatidylinositol signaling system (downregulation of glycerol-3-phosphate and myo -inositol, as well as upregulation of serine). Metabolites related to phosphorus assimilation products were impacted through downregulation of guanine , glutamine, alanine , and aspartic acid , as well as upregulation of succinic acid, thereby impeding the algal assimilation of phosphorus. Moreover, perturbation of glutathione metabolism induced by oxidative stress stimulated the alteration of membrane state (upregulation of glycine). These findings contribute to a molecular-scale perspective of nanoparticles on algae-based biotechnology in phosphorus removal.
研究领域:
环境科学
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生物技术
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代谢组学
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PubMed
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Mengen Yu,Mengjiao Chen,Jiangli Gui,Shudan Huang,Yumeng Liu,Huifei Shentu,Jian He,Zhiyu Fang,Weimin Wang,Yongjun Zhang
In the present study, six different polysaccharides (RFPs, MAPs, UWPs, AEPs, HWPs and CEPs) were extracted from Chlorella vulgaris using repeated freeze–thawing, microwave-assisted-, ultrasonic wave-, alkali-, hot water-, and cellulase-based methods; and antioxidant property assays were performed both in vitro and in vivo . Radical-scavenging capacity (using DPPH, superoxide and hydroxyl radicals) and metal chelating ability were assessed in vitro ; Caenorhabditis elegans was used to assess antioxidant effects in vivo . Based on the in vitro screening tests, UWPs exhibited high antioxidant capacity. The UWP yield was 17.1% ± 2.2%; the DPPH-, superoxide-, and hydroxyl radical-scavenging rates were 65.1% ± 2.4%, 61.2% ± 2.7%, and 56.2% ± 2.2%, respectively, and the metal chelating ability was 63.6% ± 2.5% at a concentration of 0.4 mg/mL. UWPs also exhibited high antioxidant activity in vivo . UWPs significantly increased the lifespan of C. elegans under oxidative stress induced by hydrogen peroxide compared with the control group, enhanced stress-resistance-related enzymes, including catalase and superoxide dismutase by 7.29% ± 1.8% and 24.41% ± 4.8%, respectively. The results of the present study indicate that the extraction methods of C. vulgaris polysaccharides were a key factor influencing antioxidant activity.
研究领域:
生物化学
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营养学
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分子生物学
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PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Li Bing,Wu Di,Li Yan,Shi Yan,Wang Chenlin,Sun Jiasi,Song Chunfeng
Antibiotic resistance is one of the most important environmental challenges. Microalgae has been considered as a promising green media for environmental purification. In this work, sulfadimethoxine (SDM) biodegradation potential of Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025 is investigated. Experimental results indicated that the tested freshwater and marine microalgae strains presented stress response to SDM addition. For Chlorella sp. L38, it has a good adaptability to SDM condition via antioxidant enzyme secretion (SOD, MDA, and CAT up to 23.27 U/mg, 21.99 μmol/g, and 0.31 nmol/min/mg) with removal rate around 88%. P. tricornutum MASCC-0025 exhibited 100% removal of 0.5 mg/L SDM. With increasing salinity (adding a certain amount of NaCl) of cultivation media, the removal rate of SDM by microalgae increased. Although its adaptive process was slower than Chlorella sp. L38, the salinity advantage would facilitate enzyme accumulation. It indicated that microalgae could be used to remove SDM from freshwater and marine environment via suitable microalgae strain screening.
研究领域:
环境科学
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微生物学
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PubMed
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JOURNAL OF MOLECULAR LIQUIDS
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2022
·
IF 6.633
Gul Muhammad,Jingjing Wang,Wenlong Xiong,Yongkun Lv,Shen Zhang,Anqi Zhao,Parisa Jahanbakhsh-Bonab,Alexei Solovchenko,Jingliang Xu,Md. Asraful Alam
The intention of the present study was to develop a green and sustainable extraction procedure to obtain lutein from microalgae. A series of acidic and polyol based deep eutectic solvents (DESs) were synthesized to establish a novel pretreatment process. Response surface methodology (RSM) was adopted to analyzese the significance of pretreatment parameters with high prediction levels of lutein yield. Amongst all the DESs-assisted pretreatment, polyol based ChCl:Gly (1:2) DES obtained the maximum lutein yield at the following optimal conditions: biomass-to-DES ratio, 3:34.6; temperature, 26 °C; time, 40 min. Lutein extraction was optimized by one-factor-at-a-time approach, and results showed that 1.35 mg/g lutein could obtained at 40 °C for 20 min with a biomass-to-solvent ratio of 30 mg/mL. The recyclability of the DES ChCl:Gly (1:2) was also tested and showed satisfactory results up to three cycles. The mechanism of lutein extraction using ethyl acetate was explored by molecular dynamics.
研究领域:
化学工程
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生物技术
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可持续化学
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提取技术
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微藻生物技术
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高影响因子
WATER AIR AND SOIL POLLUTION
·
2022
·Vol. 233
·期 9
·1-8
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IF 2.984
Wu Yicheng,Zhou Zhuoyi,Ou Lihui,Yu Jian,Fu Haiyan,Yang Aili
Carbamazepine, as one of the pharmaceutical and personal care products, is highly persistent in aquatic environments. This study investigated the effects of carbamazepine on the physiology and photosynthetic characteristics of Chlorella vulgaris , and the removal of carbamazepine by C. vulgaris was evaluated. The results showed that the growth and the photosynthesis activity of C. vulgaris decreased with the increase in carbamazepine concentration, while superoxide dismutase and catalase activities were markedly promoted. It was also revealed that C. vulgaris could result in efficient removal of carbamazepine with a concentration of 40 mg/L. The removal efficiency reached 79.16 ± 3.98% after 8-day exposure. Pathway analysis revealed that 77.05 ± 3.91% of carbamazepine was removed by biodegradation, and bioaccumulation and biosorption contributed only 1.47 ± 0.14% and 0.37 ± 0.06%, respectively. The research implied that biodegradation by C. vulgaris might be an efficient method to treat carbamazepine-contaminated water. Graphical abstract
研究领域:
环境科学
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毒理学
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水污染治理
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2023
·
IF 6.8
Min Li,Yayao Wang,Jianren Xu,Xiu Zhang,Zhaojun Wei
The hazardous potential of haloquinolines (HQLs) is becoming an issue of great concern due to its wide and long-term usage in many personal care products. We examined the growth inhibition, structure-activity relationship, and toxicity mechanism of 33 HQLs on Chlorella pyrenoidosa using the 72-h algal growth inhibition assay, three-dimensional quantitative structure–activity relationship (3D-QSAR), and metabolomics. We found that the IC 50 (half maximal inhibitory concentration) values for 33 compounds ranged from 4.52 to > 150 mg·L −1 , most tested compounds were toxic (1 mg·L −1 < IC 50 < 10 mg·L −1 ) or harmful (10 mg·L −1 < IC 50 < 100 mg·L −1 ) for the aquatic ecosystem . Hydrophobic properties of HQLs dominate their toxicity. Halogen atoms with large volume appear at the 2, 3, 4, 5, 6, and 7-positions of the quinoline ring to significantly increase the toxicity. In algal cells, HQLs can block diverse carbohydrates, lipids, and amino acid metabolism pathways, thereby resulting in energy usage, osmotic pressure regulation, membrane integrity, oxidative stress disorder, thus fatally damaging algal cells. Therefore, our results provide insight into the toxicity mechanism and ecological risk of HQLs.
研究领域:
环境科学
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毒理学
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水生生态学
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化学信息学
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PubMed
高影响因子
Materials
·
2023
·Vol. 16
·期 10
·3642
·
IF 3.4
Lina Zhang,Xiaomin Huo,Jin Zhu,Changbin Liu,Lianfeng Wang
Dye is an anionic common pollutant in industrial wastewater and poses a great threat to the environment and human health. Owing to its advantageous adsorption capacity, nanocellulose is widely used for wastewater treatment. The cell walls of Chlorella mainly comprise cellulose instead of lignin. In this study, residual Chlorella-based cellulose nanofiber (CNF) and cationic cellulose nanofiber (CCNF) with surface quaternization were prepared through homogenization. Moreover, Congo red (CR) was used as a model dye to measure the adsorption capacity of CNF and CCNF. The adsorption capacity was almost saturated when CNF and CCNF contacted CR for 100 min, and the adsorption kinetics coincided with the pseudo-secondary kinetics model. The initial concentration of CR considerably affected its adsorption on CNF and CCNF. Below the initial concentration of 40 mg/g, the adsorption on CNF and CCNF considerably increased with the increase in the initial concentration of CR. Based on the sorption isotherms analysis of CNF and CCNF, the Langmuir model fitted best with the experimental data. Thus, CNF and CCNF surfaces were uniform, and monolayer adsorption occurred. The adsorption of CR on CNF and CCNF was greatly affected by the pH value, and the acidic medium favored the adsorption of CR (especially for CCNF). CCNF showed a more advantageous adsorption capacity, with a maximum value of 1657.89 mg/g, compared to that of CNF (190.0 mg/g). According to the findings of this study, residual Chlorella-based CCNF could be a very promising adsorbent candidate for removing anionic dyes from wastewater.
研究领域:
环境科学
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材料科学
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ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Juncheng Zheng,Han Song,Yuankun Yang,Haidong Li,Xuemei Luo,Shu Chen
Persulfate (PDS)-based advanced oxidation processes have emerged as promising methods for antibiotic removal, but achieving high catalytic efficiency for tetracycline (TC) degradation remains difficult. The catalytic performance of FeGCB-N in PDS activation depended on Fe content and pyrolysis temperature, with defect density and intermetallic compound formation largely determined by the pyrolysis temperature. The optimized Fe 0.2 GCB-N-800 exhibited remarkable PDS activation performance for tetracycline degradation, achieving over 99% removal within 65 min under conditions of 10 mg·L -1 TC, 1 mM PDS, and pH 3. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) collectively indicate that surface oxygen-containing functional groups, defect sites, and intermetallic Fe compounds provide active sites for PDS activation by FeGCB. Electron paramagnetic resonance (EPR) and quenching results demonstrated that superoxide radicals (O 2 •- ) and sulfate radicals (SO 4 •− ) were the primary reactive species in TC degradation. Density functional theory (DFT) calculations indicate that Fe-N is the key active site for PDS activation. Based on DFT results and liquid chromatography-mass spectrometry (LC-MS) analysis, potential degradation pathways for tetracycline (TC) are proposed. ECOSAR toxicity assessment showed that the O 2 •- -mediated pathway would generate fewer toxic byproducts. This work offers an effective approach to design environment friendly catalysts from algae biomass for treating wastewater.
研究领域:
环境科学
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材料化学
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水处理技术
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催化
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废弃生物质利用
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PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
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2026
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IF 4.5
Xiaotong Zou,Shaohua Zhao,Ke Liu,Lingtao Li,Miaosen Zhang,Rui Lu,Changqing Fang
The increasing environmental burden associated with petroleum-based plastic packaging has stimulated growing interest in microalgae-derived bio-based materials for sustainable food packaging applications. In this study, active composite films based on chitosan (CS) and Chlorella vulgaris (CV) oil were prepared using a solution-casting method. Scanning electron microscopy (SEM) observations indicated that the incorporation of CV oil significantly altered the film morphology, resulting in uniformly distributed porous microstructures. Mechanical property analysis showed a gradual decrease in tensile strength, accompanied by an initial increase followed by a decline in elongation at break, with a maximum enhancement of 66%. The surface hydrophobicity of the films increased with increasing CV oil content, as evidenced by an increase in water contact angle up to 83.99°, while water solubility, swelling ratio, and water vapor permeability were correspondingly reduced. Optical measurements revealed a pronounced decrease in UV–visible light transmittance, indicating improved light-barrier performance. Furthermore, bioactive compounds present in CV oil enhanced the antioxidant and antimicrobial properties of the films, with the DPPH radical scavenging activity increasing from 43.17% to 73.45% and effective inhibition observed against Escherichia coli and Staphylococcus aureus . These results demonstrate the potential of CV oil as a functional microalgae-derived additive for the development of sustainable and bioactive packaging films.
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JOURNAL OF THERMAL SPRAY TECHNOLOGY
·
2020
·Vol. 29
·期 8
·1991-2000
·
IF 2.522
Zhao Hang,Zhang Haijun,Chen Xiuyong,McDonald André,Li Hua
Thermal-sprayed aluminum (Al) coatings are widely used for corrosion protection in marine environments. The corrosive nature of seawater and the synergy with marine microbes make for aggressive service environments for Al coatings. In this study, Al coatings were deposited on 316L stainless steel (316L SS) substrates by using wire arc spraying. Chlorella vulgaris , a typical marine fouling algae, was used to investigate the effects of biofilm adhesion on the electrochemical behavior of wire arc-sprayed Al coatings in artificial seawater environment. Electrochemical measurements via dynamic potential polarization curves and electrochemical impedance spectroscopy were used to monitor the corrosion behavior after attachment of microbes on Al coatings and bare, uncoated 316L SS. Analysis of the corrosion products was conducted using scanning electron microscope and energy-dispersive spectrum. The results showed that the Chlorella vulgaris biofilm slowed the corrosion of the Al coatings. This suggested that Chlorella vulgaris biofilm was a critical contributing factor to the corrosion behavior of the Al coatings. Moreover, a mechanism was suggested to illustrate the corrosion behavior of Al coatings in the presence of Chlorella vulgaris .
研究领域:
材料科学
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海洋生物学
·
电化学
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高影响因子
Carbon Neutralization
·
2024
·
IF 0
Hua-Wei Guo,Ya-Jun Wang,Huan Liu,Ya-Nan Zeng,Wei-Jie Wang,Tian-Ji Liu,Le-Le Kang,Rui Ji,Yi-Tong Wang,Jun-Guo Li,Zhen Fang
Metallurgical slag such as solid waste generated in the steel industry carries environmental pollution risks, but it is rich in nutrients required by microalgae. Metallurgical slag used for carbon capture and biomass energy conversion has multiple benefits: (i) reduction and harmless treatment of metallurgical solid waste, (ii) assisting in carbon neutrality by efficient carbon fixation, and (iii) production of biodiesel from CO 2 . In this study, AOD, BOF, BFS, HVS, and VTS slag were applied to culture Chlorella pyrenoidosa ( C. pyrenoidosa ) with the regulation of growth, carbon fixation, and lipid synthesis. An excellent fixed amount of CO 2 with 94.59 mg is obtained from C. pyrenoidosa biomass at BOF slag added (mass ratio of CO 2 captured/microalgae/slag with 1.99/1.00/10.53) since high Ca/Mg mass ratio of 419 (8.38 mg/L Ca and 0.02 mg/L Mg), no Cr and low concentration of Al (0.04 mg/L) contribute to regulating antioxidant enzyme activity (SOD and POD) to resist ROS and improving PEPC activity to reduce carbon flux toward lipid to promote biomass synthesis. Both metal concentrations from Ca (5.86 mg/L), Mg (0.05 mg/L), Al (0.42 mg/L), and Cr (0.006 mg/L) and suitable pH (10.53) in AOD leaching solution at solid/liquid ratio of 0.5 g/L change carbon flow toward efficient lipid synthesis (47.07 wt%) by continuously providing raw materials and energy by regulating ACC, ME, and PEPC activities. High value-added biodiesel with high concentrations of C16 and C18 methyl esters from lipid of C. pyrenoidosa is achieved, following other ecological and economic benefits including 197 mg CO 2 captured and 2198 mg AOD applied with harmless. In this study, C. pyrenoidosa is cultured with elements from metallurgical slag solid waste, which promotes C. pyrenoidosa efficient carbon fixation to assist in carbon neutrality, and provides guidance for CO 2 conversion to high-value-added products with low cost.
研究领域:
环境科学
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能源工程
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生物技术
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材料科学
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危险品化学品经营许可证（带存储）