FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Ruican Wang,Yawei Chang,Yuanyifei Wang,Richard W. Hartel,Ying Zhang,Liye Zhu,Dancai Fan,Shuo Wang
The study investigates the gelation kinetics of gelatin in the presence of various saccharides, including common sugars (sucrose, fructose, & glucose), rare sugars (ribose, allulose, isomaltulose, & trehalose), and prebiotic trisaccharides (raffinose and 2′-fucosyllactose). Gelation kinetics, including the initial sol-gel transition and subsequent aging, was monitored using a rheometer, while gel properties were assessed through texture analysis, differential scanning calorimetry, and centrifugation. The effects of these cosolutes on sol-gel transition varied depending on their molecular weight and stereochemistry, but they generally hindered gel network aging due to increased bulk viscosity. Ribose and allulose inhibited gelatin sol-gel transition, resulting in weak gel strength at all concentrations. Arabinose and raffinose crystallized rapidly at high concentrations, damaging gel structures. At low to medium concentrations (10–30 %), di- and trisaccharides accelerated gelation, but at higher levels, increased bulk viscosity impeded gel percolation, reducing gel strength. In terms of gel-stabilizing forces, the addition of sucrose promoted electrostatic interactions but weakened hydrogen bonding within gel network. This work emphasizes the need to account for gelatin-to-water ratio when evaluating cosolute effects. The findings provide valuable insights for sugar reduction and functionalization for gelled foods.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhipeng Zhu,Yuqian Xiang,Xiaohui Yan,Chenglong Shi,Cheng Chen,Hongxing Liu,Bin Wang,Yanshuo Li,Dapeng Wu
Crosslinked and uncrosslinked polyacrylamide stationary phases were prepared on porous silica gel (SiL-PAAm) by atom transfer radical polymerization (ATRP), and their hydrophilic interaction liquid chromatography (HILIC) separation performance and stability were evaluated systematically. After aminosilylation and esterification with 2-bromoisobutyryl bromide on silica gel, surface-initiated ATRP with acrylamide or acrylamide/N,N’-methylenebisacrylamide mixtures at molar ratios of 1:0.1 and 1:0.2 was carried out, and SiL-PAAm-0, SiL-PAAm-10, and SiL-PAAm-20 HILIC materials were obtained. SiL-PAAms were characterized by FT-IR, TGA and nitrogen adsorption–desorption isotherms. It was confirmed that these three SiL-PAAms had very similar specific surface area of 130–143 m 2 g −1 , pore size of 8.67–8.89 nm, and PAAm bonding amount of 177–181 mg g −1 . Their HILIC performances were evaluated and compared by separating nucleosides, vitamins, sugars, and amino acids, respectively. It was found that SiL-PAAms exhibited significantly stronger retention and higher separation selectivity than both bare silica and commercial amide columns. However, an excessive increase in the crosslinking degree also led to a decrease in retention strength. Additionally, the SiL-PAAm-10 and SiL-PAAm-20 exhibited significantly reduced column bleeding and enhanced reproducibility, far surpassing both SiL-PAAm-0 and commercial amide columns. Generally, the suitably crosslinked PAAm by ATRP has been proven as one highly efficient and robust HILIC stationary phase for the chromatographic separation of a wide range of polar compounds.
✓ 已验证
产品关联
DOI
高影响因子
ACS Synthetic Biology
·
2025
·
IF 3.9
Di Liu,Long Wang,Linbo Gou,Yao Lu,Yongai Ma,Songsong Yao,Tai-Ping Fan,Huaxiang Deng,Yujie Cai
Terpenoids are valuable chemicals used across industries. Methanol, a nonsugar-based feedstock, offers an eco-friendly approach to terpenoid production. In this study, a hybrid methylotrophic pathway was engineered in Serratia marcescens (S. marcescens) HBQA7, which is tolerant to both methanol and terpenoid compounds. The pathway utilizes bacterial methanol dehydrogenase (Mdh) and yeast dihydroxyacetone synthase (Das) to produce monoterpenes and sesquiterpenes from methanol and xylose. 13C labeling experiments identified the optimal enzyme pair: Mdh from Acinetobacter gerneri and Das from Pichia angusta, achieving 7.63% 13C enrichment of the central metabolic intermediate pyruvate. Deletion of pentose phosphate pathway genes (rpiAB) enhanced methanol utilization, achieving 22.99% 13C enrichment. Optimization of the mevalonate (MVA) biosynthetic pathway enabled the production of 5.12 g/L mevalonate in shake flask culture from methanol and xylose. Further construction of a haloarchaea-type MVA pathway enabled the production of geraniol (574.12 mg/L) and (−)-α-bisabolol (1256.41 mg/L) in shake flask cultures. This study demonstrates the first methanol conversion into valuable terpenoids in S. marcescens.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Minghao Fan,Yudi Jiang,Chao Cai,Zhe Wang,Lu Chen,Xin Zhang,Hua Yin,Shumin Hu,Jia Liu,Zhonghua Qian,Shuli Huang
Cognitive impairment associated with natural aging significantly reduces the healthy lifespan of elderly adults. Barley is rich in polysaccharides, particularly starch and dietary fibers such as β-glucan and xylan. As the predominant components of barley water extracts, these polysaccharides, especially dietary fibers, exhibit substantial potential in promoting gut and brain health. In this study, we established a natural aging model by exposing mice to a high-fat diet and chronic stress for 220 consecutive days. Our findings revealed that barley polysaccharides ameliorated cognitive deficits, particularly long-term memory, by modulating neurotransmitter levels and reducing corticosterone. Barley polysaccharides also alleviated lipid metabolism disorders, reduced liver lesions, and decreased body weight as well as the percentage of visceral fat in mice by regulating bile acid and l -lysine metabolism. Additionally, barley polysaccharides enhanced intestinal barrier integrity and reshaped the gut microbiota. They significantly increased the abundance of norank_f_Muribaculaceae and unclassified f_Lachnospiraceae , leading to elevated short-chain fatty acid levels, especially butyric acid, which contributed to improved cognitive function. These findings suggest that barley polysaccharides could serve as a promising dietary intervention to mitigate cognitive decline associated with natural aging through the liver-gut-brain axis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 23
·4123
·
IF 5.1
Lili Zhao,Mengrong Chen,Chunjing Fu,Tao Pan,Qiling Chen
Microorganism employs sophisticated strategies to adapt to acidic environments, with transcription factors occupying pivotal nodes within their hierarchical regulatory networks. In this study, we performed functional characterization of the AraR transcription factor LP_RS14895 via integrated multiomics approaches. RNA sequencing revealed 40 acid-responsive targets that were enriched in pathways related to pentose/glucuronate interconversions and amino sugar and nucleotide sugar metabolism. A genome-wide binding analysis via DAP-seq identified 1279 interaction sites and the most significantly enriched motif is “ARCCMATMAHC”. The results revealed that AraR plays a crucial role in regulating acid tolerance and metabolizable sugar (including arabinose, glucose, fructose, ribose, mannose, and trehalose). Overall, these findings offer mechanistic insights into microbial stress responses and provide a valuable method for addressing inhibitory processes of carbohydrate metabolizability under high-acid conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 23
·4126
·
IF 5.1
Yuemou Zhao,Wenyu Liang,Huaqun Chen,Jinwen Huang,Longyan Zhao,Qingxia Yuan
Heteropolysaccharides, the principal bioactive constituents of the esteemed medicinal foodTremella aurantialba, remain poorly understood in both structure and function. Herein, we describe a novel heteropolysaccharide, designated TAP-2a, isolated from the fruiting bodies ofT. aurantialbavia multi-step column chromatography. With a molecular weight of 16.95 kDa, TAP-2a is dominated by the pyranose forms of ᴅ-galactose (ᴅ-Galp), ᴅ-glucose (ᴅ-Glcp) and ᴅ-mannose (ᴅ-Manp), accompanied by minor proportions of ᴅ-xylose (ᴅ-Xylp), ʟ-fucose (ʟ-Fucp) and glucuronic acid. Methylation-GC-MS and exhaustive 1D/2D NMR analyses revealed a backbone assembled from →6)-α-Galp-(1→, →6)-β-Glcp-(1→, and →3)-α-Manp-(1→residues, branched at →2,6)-β-Galp-(1→, →3,6)-α-Galp-(1→, and →2,3)-α-Manp-(1→residues, and terminated by β-Glcp-(1→, α-Fucp-(1→, and β-Xylp-(1→. This intricate glycosidic architecture generates an exceptionally complex mannoglucogalactan in which a Gal→Man domain is substituted atO-3 of Gal by t-β-Glcpside chains and atO-2 of Man by t-α-Fucpstubs; additionally, a discrete fragment comprising t-β-Glcp-(1→3)-β-Glcp-(1→ was identified, along with a minor branch in which t-β-Xylpis attached toO-2 of a mannose residue. Functionally, TAP-2a proved to be a potent immunomodulator, markedly enhancing the secretion of nitric oxide, interleukin-1β, interleukin-6 and tumour necrosis factor-α while concurrently up-regulating the corresponding mRNA transcripts and augmenting phagocytic capacity. These findings establish the highly elaborate heteropolysaccharides ofT. aurantialbaas powerful immunomodulators that underpin the fungus’s renowned medicinal efficacy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·
IF 15.7
Chen Long,Zhang Zonghao,Li Penghao,Luo Hanzhuo,Li Chenchen,Pan Sihui,Huang Wei-Hsiang,Pao Chih-Wen,Yang Hao,Hu Zhiwei,Shao Qi
Electrocatalytic acetone hydrogenation into high-value alcohols offers a sustainable alternative to thermal methods. However, developing non-noble metal catalysts with superior performance for electrocatalytic acetone hydrogenation remains challenging. Two-dimensional non-noble metal structure with metastable-phase active sites is promising due to its high-energy structure and unique electronic environment. However, its precise synthesis remains a great challenge. In this work, we report a two-dimensional metastable hexagonal close-packed phase nickel nanosheets (hcp Ni NSs) with a space group of P6 3 /mmc. In the electrocatalytic acetone hydrogenation, it achieves 95.0 % acetone conversion with 100 % isopropanol selectivity. Notably, in the scale-up experiment, the hcp Ni NSs also exhibit 11.51 g g cat -1 h -1 isopropanol production rate at a current density of −250 mA cm -2 . Furthermore, this catalyst is widely applicable to various carbonyl compounds. Mechanism study shows that hcp Ni NSs enable strong acetone adsorption and rapid isopropanol desorption.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Jinwen Huang,Huiqi Wang,Huaqun Chen,Zidong Liu,Xuedong Zhang,Hao Tang,Shiying Wei,Wangting Zhou,Xinzhou Yang,Yonghong Liu,Longyan Zhao,Qingxia Yuan
Avicennia marina (Forssk.) Vierh. is a highly salt-tolerant mangrove, and its fruit has been traditionally used for treating constipation and dysentery . In this study, a pectin (AMFPs-0-1) was extracted and isolated from this fruit for the first time, its structure was analyzed, and the effects on the human gut microbiota were investigated. The results indicated that AMFPs-0-1 with a molecular weight of 798 kDa had a backbone consisting of alternating →2)-α-L-Rha p -(1→ and →4)-α-D-Gal p A-(1→ residues and side chains composed of →3-α-L-Ara f -(1→-linked arabinan with a terminal β-L-Ara f , →5-α-L-Ara f -(1→-linked arabinan, and →4)-β-D-Gal p -(1→-linked galactan that linked to the C-4 positions of all α-L-Rha p residues in the backbone. It belongs to a type I rhamnogalacturonan (RG-I) pectin but has no arabinogalactosyl chains. AMFPs-0-1 could be consumed by human gut microbiota and increase the abundance of some beneficial bacteria, such as Bifidobacterium , Mitsuokella , and Megasphaera , which could help fight digestive disorders . These findings provide a structural basis for the potential application of A. marina fruit RG-I pectic polysaccharides in improving human intestinal health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Qingxia Yuan,Rongyi Liang,Kunling Lv,Xiaohuo Shi,Jing Leng,Yonghong Liu,Jian Xiao,Lifeng Zhang,Longyan Zhao
Chlorella polysaccharides have been gaining increasing attention because of their high yield from dried Chlorella powder and their remarkable immunomodulatory activity. In this study, the major polysaccharide fraction, CPP-3a, in Chlorella pyrenoidosa , was isolated, and its detailed structure was investigated by analyzing the low-molecular-weight product prepared via free radical depolymerization. The results indicated that CPP-3a with a molecular weight of 195.2 kDa was formed by →2)-α-L-Ara f -(1→, →2)-α-D-Rha p -(1→, →5)-α-L-Ara f -(1→, →3)-β-D-Glc p- (1→, →4)-α-D-Glc p- (1→, →4)-α-D-Glc p A-(1→, →2,3)-α-D-Man p- (1→, →3,4)-α-D-Man p- (1→, →3,4)-β-D-Gal p- (1→, →3,6)-β-D-Gal p- (1→, and →2,3,6)-α-D-Gal p- (1→ residues, branched at C2, C3, C4, or C6 of α/β-D-Gal p and α-D-Man p , and terminated by α/β-L-Ara f , α-L-Ara p , α-D-Gal p , and β-D-Glc p . Biological assays showed that CPP-3a significantly altered the dendritic morphology of immature dendritic cells (DCs). Enhanced CD80, CD86, and MHC I expression on the cell surface and decreased phagocytic ability indicated that CPP-3a could induce the maturation of DCs. Furthermore, CPP-3a-stimulated DCs not only stimulated the proliferation of allogeneic naïve CD4 + T cells and the secretion of IFN-γ, but also directly stimulated the activation and proliferation of CD8 + T cells through cross-antigen presentation. These findings indicate that CPP-3a can promote human DC maturation and T-cell stimulation and may be a novel DC maturation inducer with potential developmental value in DC immunotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongchun Huang,Jie Bai,Jinman Shui,Zhanhai Su,Chengzhu Cao,Haiyan Wang,Qiong Wu,Shoude Zhang
Analysing monosaccharide composition is an essential step in deciphering polysaccharide structures. However, it presents significant challenges due to the pronounced hydrophilicity and the vast array of monosaccharide isomers. Gas/liquid chromatography, coupled with an aldononitrile acetate derivatisation, has been widely used for monosaccharide composition analysis. However, it poses considerable difficulties in simultaneously determining the D/L configurations of these monosaccharides. In this study, a novel method that can simultaneously determine the composition of polysaccharides and their D/L configuration based on L-cysteine methyl ester derivatisation (CMED) and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the comprehensive analysis of monosaccharide types. This method enables the simultaneous characterization of aldoses, ketoses, alditols, and uronic acids. Furthermore, the reaction mechanisms and by-products were meticulously analysed, offering a robust theoretical basis for this novel methodology. Additionally, the developed method was employed to characterize the monosaccharide composition for six plant polysaccharides. Ultimately, the findings demonstrated that the proposed method is effective for confirming monosaccharide composition, encompassing both structural types and absolute configurations.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Natural Products and Bioprospecting
·
2024
·Vol. 14
·期 1
·1-11
·
IF 4.8
Huang Ni,Yang Yi-Na,Huang Jia,Shao Hui-Yan,Li Yan-Lang,Qin Shi-Hui,Li Han-Fen,Shen Xiao-Jiang,Yang Liu,Hu Jiang-Miao
Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata . Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10 3 and 1.25 × 10 4 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)- α -D-Gal p -(1 → 6)- α -D-Gal p -(2,1 → 6)- α -D-Man p -(2,1 → 6)- α -D-Gal p -(1, and branch chains were β -D-Xyl p -(1 → 3)- α -L-Fuc p -(1 → 4)- α -D-Man p -(1 → and α -D-Gal p -(1 → 3)- α -D-Gal p -(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-24
·
IF 12.6
Yu Yang,Kong Liang,Guo Rui-bo,Zhang Ya-ni,Li Shu-tong,Zhang Feng-yuan,Wang Xin,Liu Yang,Li Xiu-Ying,Li Xue-tao
Background The imbalance of macrophage polarization plays a pivotal role in the progression of rheumatoid arthritis (RA). Reprogramming macrophages from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype is considered a promising therapeutic strategy. Methods To address this challenge, Panax notoginseng polysaccharides (PNP) with varying molecular weights were chemically conjugated with deoxycholic acid (DC) to obtain amphiphilic conjugates (PNP-DC), which self-assembled into micelles (PNP-Ms). After screening for optimal molecular weight, folic acid (FA) was introduced onto the micelle surface, and Polyphyllin I (PPI) was encapsulated to form FA-modified, PPI-loaded micelles (FA-PPI-Ms) with macrophage-targeting capability. Results FA-PPI-Ms showed enhanced cellular uptake via FA receptor–mediated endocytosis and effectively eliminated reactive oxygen species (ROS), reduced inflammatory cytokine production, and exhibited good biosafety. In vivo, FA-PPI-Ms significantly alleviated joint swelling and inflammation in RA rat models. Mechanistic studies based on RNA sequencing and experimental validation revealed that FA-PPI-Ms suppressed the JAK2/STAT3 signaling pathway, thereby promoting M2 macrophage polarization and restoring the M1/M2 balance. Conclusion This study presents a novel FA-PPI-Ms delivery system for targeted macrophages. By modulating polarization through inhibition of JAK2/STAT3 signaling, the system offers a promising therapeutic strategy for RA and potentially other inflammatory diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）