查找使用阿拉丁产品的科研文献

Multiscale structured MOF-808@PVDF/DA fiber membranes with high filtration efficiency and long-term loading capacity for air-filtration applications

Xinyu Ren,Weili Shao,Kaiyang Cao,Ningbo Zhao,Jingyu Ma,Weichang Xiao,Ying Cao,Fan Liu

Existing air filtration materials suffer from short service life, limited load capacity, and lack of multifunctional properties such as chemical degradation resistance. To address these limitations, this study combined electrostatic-assisted solution blow spinning (E-SBS) with in situ growth of metal-organic frameworks (MOFs) to produce a scalable MOF-808@polyvinylidene fluoride/dopamine 2 (MOF-808@PVDF/DA2) micro/nanofiber composite membrane. The multiscale PVDF/DA micro-nanofiber composite membrane produced via electrostatic-assisted solution blow spinning exhibits small pore size (0.67 μm) and high porosity (92.23 %). Following dopamine (DA) modification and MOF-808 loading, the membrane exhibits outstanding filtration performance (filtration efficiency: 99.98 %, quality factor Q f : 0.129 Pa −1 ), long-term dust-holding capacity (dust-holding capacity up to 22.9 g m −2 ), and excellent catalytic degradation performance (98.7 % degradation rate within 30 min). Notably, the MOF-808@PVDF/DA2 membrane exhibits high air permeability (158 mm s −1 ), effectively reducing energy consumption during filtration. After multiple cycles of use, filtration efficiency remains as high as 98 %, demonstrating a long service life. This study proposes an innovative strategy for designing next-generation protective materials that integrate filtration and catalytic detoxification functions.

Network Pharmacology and Bioactive Equivalence Assessment Integrated Strategy Driven Q-markers Discovery for Da-Cheng-Qi Decoction to Attenuate Intestinal Obstruction

Danting Li,Bo Lv,Di Wang,Doudou Xu,Siyuan Qin,Ying Zhang,Jie Chen,Wei Zhang,Zunjian Zhang,Fengguo Xu

ABSTRACT Background Intestinal obstruction (IO) is a kind of acute abdomen with high morbidity and mortality. Patients suffer from poor quality of life and tremendous financial pressure. Da-Cheng-Qi decoction (DCQD), a classical purgation prescription, has clinically been proven to be an effective treatment for IO. Purpose Network pharmacology integrated with bioactive equivalence assessment was used to discover the quality marker (Q-marker) of DCQD against IO. Methods As there is hardly any targets recorded in database, thus the collection of IO targets was conducted by searching those of alternative diseases which have similar pathological symptoms with IO. In order to improve the reliability of the obtained targets, IO metabolomics data was introduced. Active compounds combination (ACC) was focused as potential Q-markers via component-target network analysis and function query from the identified components corresponding to the common targets. Bioequivalence between ACC and DCQD was assessed from the aspects of intestine motility (somatostatin secretion), inflammation (IL-6 secretion) and injury (wound healing assay) in vitro and was further validated in ileus rat model. PPI network analysis of core targets followed by gene pedigree classification and experimental validation confirmed the potential intervention pathway. Results A combination of 11 ingredients, including emodin , physcion , aloe-emodin, rhein , chrysophanol , gallic acid , magnolol , honokiol , naringenin , tangeretin , and nobiletin was finally confirmed bioequivalence with DQCD to some extent and could serve as Q-markers for DCQD to attenuate IO. PI3K/AKT was verified as a possible affected pathway that DCQD exerted the effectiveness against IO. Conclusion For the disease with few recorded targets, searching those of alternative diseases which have similar pathological symptoms could be a feasible and effective approach. The proposed network pharmacology integrated bioactive equivalence evaluation paradigm is efficient to discover Q-marker of herbal formulae.

Therapeutic Effect and Mechanism of Da-Huang-Zhe-Chong Pills on Uterine Fibroids Using Liquid Chromatography Combined With Mass Spectrometry Metabolomics

Yonghua Hu,Xujin Yang,Chenmei Ma,Zhen Wang,Xin Yuan,Man He,Penghai Zhang,Yi Tao

Da-Huang-Zhe-Chong Pills (DHZCPs) have demonstrated efficacy in treating uterine fibroids (UFs), but the mechanisms underlying their action remain unclear. In this study, untargeted serum metabolomic analysis was employed to investigate the therapeutic effects of DHZCP in a rat model of UFs utilizing advanced ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and pattern recognition. Histopathological examination through H&E staining revealed significant improvements in uterine morphology following DHZCP administration. The levels of four serum hormones, that is, estradiol (E 2 ), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone (PROG), tended to shift toward normal in the DHZCP-treated group compared with the model group. Our metabolomic profiling identified 20 distinct endogenous metabolites that were differentially expressed between UF and normal rats, with DHZCP treatment significantly normalizing 16 of these metabolic markers. Comprehensive pathway analysis highlighted three major metabolic pathways affected by DHZCP intervention: tryptophan metabolism, riboflavin metabolism, and arginine-proline metabolism. Notably, spearman correlation analysis indicated that tryptophan, lysoPC (18:1/0:0), uric acid, and serotonin were strongly positively correlated with serum levels of both E 2 and PROG. Additionally, riboflavin, indoleacetic acid, and indole-3-propionic acid were positively correlated with E 2 levels. Our findings suggest that DHZCP may exert therapeutic effects primarily through the modulation of tryptophan and riboflavin metabolism, providing a solid foundation for its clinical application.

Ultrastretchable, self-adhesive, strain-sensitive and self-healing GO@DA/Alginate/P(AAc-co-AAm) multifunctional hydrogels via mussel-inspired chemistry

Xiaoqiang Jin,Huihong Jiang,Zhiming Zhang,Yuejun Yao,Xiaojiong Bao,Qiaoling Hu

For conductive hydrogels applied in biosensors, wearable devices and so forth, multifunctionality is an inevitable trend of development to meet various practical requirements and enhance human experience. Herein, inspired by nanocomposite, double-network (DN) and mussel chemistry, a new Graphene oxide@Dopamine/Alginate/Poly(acrylic acid- co -acrylamide) [GO@DA/Alginate/P(AAc- co -AAm)] hydrogel was fabricated through one-pot in-situ radical copolymerization. GO@DA nanofillers, prepared via GO confined DA polymerization, imparted the hydrogel with remarkable adhesiveness. Alginate/P(AAc- co -AAm) DN matrix, physically and chemically crosslinked by Fe 3+ and N,N'-Methylenebisacrylamide, made hydrogels ultrastretchable, self-healing and biocompatible. With contents of DA and alginate accurately regulated, the tensile strength, elongation, adhesion strength and conductivity of the optimal hydrogel could reach 320.2 kPa, 1198 %, 36.9 kPa and 3.24 ± 0.12 S/m, respectively. What’s more notable was that the synergistic integration of repeatable adhesiveness, strain sensitivity, use stability, self-healing ability and biocompatibility provided such hydrogels with tremendous possibility of practical application for strain sensors.

Identification and Quantitation of Melamine in Milk by Near-Infrared Spectroscopy and Chemometrics

Tong Wu,Hui Chen,Zan Lin,Chao Tan

Melamine is a nitrogen-rich substance and has been illegally used to increase the apparent protein content in food products such as milk. Therefore, it is imperative to develop sensitive and reliable analytical methods to determine melamine in human foods. Current analytical methods for melamine are mainly chromatography-based methods, which are time-consuming and expensive and require complex pretreatment and well-trained technicians. The present paper investigated the feasibility of using near-infrared (NIR) spectroscopy and chemometrics for identifying and quantifying melamine in liquor milk. A total of 75 samples were prepared. Uninformative variable elimination-partial least square (UVE-PLS) and partial least squares-discriminant analysis (PLS-DA) were used to construct quantitative and qualitative models, respectively. Based on the ratio of performance to standard deviate (RPD), UVE-PLS model with 3 components resulted in a better solution. The PLS-DA model achieved an accuracy of 100% and outperformed the optimal reference model of soft independent modeling of class analogy (SIMCA). Such a method can serve as a potential tool for rapid screening of melamine in milk products.

Critical new insights into the interactions of hexafluoropropylene oxide-dimer acid (GenX or HFPO-DA) with albumin at molecular and cellular levels

Shi-Ya Peng,Ya-Di Yang,Rong Tian,Naihao Lu

A key characteristic to be elucidated, to address the harmful health risks of environmental perfluorinated alkyl substances (PFAS), is their binding modes to serum albumin, the most abundant protein in blood. Hexafluoropropylene oxide-dimer acid (GenX or HFPO-DA) is a new industrial replacement for the widespread linear long-chain PFAS. However, the detailed interaction of new-generation short-chain PFAS with albumin is still lacking. Herein, the binding characteristics of bovine serum albumin (BSA) to GenX were explored at the molecular and cellular levels. It was found that this branched short-chain GenX could bind to BSA with affinity lower than that of legacy linear long-chain perfluorooctanoic acid (PFOA). Site marker competitive study and molecular docking simulation revealed that GenX interacted with subdomain IIIA to form BSA-GenX complex. Consistent with its weaker affinity to albumin protein, the cytotoxicity of branched short-chain GenX was less susceptible to BSA binding compared with that of the linear long-chain PFOA. In contrast to the significant effects of strong BSA-PFOA interaction, the weak affinity of BSA-GenX binding did not influence the structure of protein and the cytotoxicity of GenX. The detailed characterization and direct comparisons of serum albumin interaction with new generation short-chain GenX will provide a better understanding for the toxicological properties of this new alternative.

Da-Jian-Zhong decoction restores the gut-brain balance in diarrhea-predominant irritable bowel syndrome rats via gut microbiota-bile acid axis

Huan-qing Gao,Ning Zhang,Ge-ning Li,Li Wen,Yao Li,Min Li,Tai-wei Dong,Lian Zhong,Da-neng Wei,Meng-meng Zhang,Li Ou

Ethnopharmacological relevance Da- Jian-Zhong decoction (DJZD), a Chinese medicinal formula, exhibits therapeutic effects in the clinical management of diarrhea-predominant irritable bowel syndrome (IBS-D). Accumulating studies have demonstrated that DJZD can regulate the gut-brain balance in IBS-D model rats; however, its underlying molecular mechanism remains unclear. Aim of the study To explore the potential mechanism by which DJZD restores the gut-brain axis in IBS-D model rats. Materials and methods Firstly, the therapeutic effects of DJZD in IBS-D model rats were evaluated based on the Bristol stool scale, abdominal withdrawal reflex (AWR) score, open field test (OFT) behavior, intestinal barrier integrity, and brain-gut peptide levels. Moreover, alterations in gut microbiota was detected using sequencing of the whole microbial genome. Subsequently, fecal microbiota transplantation (FMT) was performed to explore whether the regulatory effect of DJZD on the gut-brain axis against IBS-D was dependent on gut microbiota. The UHPLC-QTRAP-MS/MS was utilized to analyze bile acids (BAs) profiles and identify gut microbiota-derived BAs metabolites responsible for the beneficial effects of DJZD. Finally, the role of BAs metabolites in restoring the gut-brain axis was further verified using the IBS-D model rats. Results Treatment with DJZD not only decreased AWR score, improved stool score and intestinal barrier integrity, but also enhanced sucrose preference and changed OFT performance of IBS-D rats. Meanwhile, DJZD normalized the levels of 5-hydroxytryptamine (5-HT) and substance P (SP) in both the colon and hippocampus. Fecal microbiota harvested from DJZD-treated rats also reversed these IBS-D-associated pathological manifestations. Moreover, DJZD notably elevated the levels of secondary BAs (SBAs) in colonic contents of IBS-D rats, particularly norchenodeoxycholic acid (NorDCA), lithocholic acid (LCA), deoxycholic acid (DCA), and 3β-ursodeoxycholic acid (3β-UDCA). The elevated SBAs were positively correlated with the relative abundances of Akkermansia sp., Ruminococcus sp., and Clostridium sp. This could be attributed to the ability of these bacteria to produce bile salt hydrolases (BSH) and 7α-hydroxysteroid dehydrogenases (7α-HSDH). Additionally, administration of an LCA and DCA mixture was found to improve intestinal and brain dysfunction and restored abnormal 5-HT and SP levels in IBS-D rats. Conclusion DJZD restores gut-brain balance in IBS-D rats by regulating gut microbiota-bile acid axis, which provides a promising therapeutic strategy for IBS-D via targeting bile acid metabolites and intestinal bacteria.

Cang-ai volatile oil improves depressive-like behaviors and regulates DA and 5-HT metabolism in the brains of CUMS-induced rats

Bojun Chen,Jijun Li,Yuhuan Xie,Xi Ming,Gang Li,Jinjin Wang,Meng Li,Xiaohong Li,Lei Xiong

Ethnopharmacological relevance Cang-ai volatile oil (CAVO) is a traditional Chinese medicine (TCM) inhalational preparation for the treatment of some depressive and emotive disorders. Aim of the study This research aimed to evaluate the efficiency and possible mechanism of intranasal CAVO administration on depression in chronic unpredictable mild stress (CUMS)-induced rats compared to lavender volatile oil (LVO) treatment after CUMS exposure and bilateral olfactory bulb impairment (OBI) in rats. Materials and methods Forty depressive-like model rats induced by CUMS were evaluated by the forced swim test (FST), open field test (OFT), and sucrose preference test (SPT). The model rats were divided into five groups: CUMS (n = 8), CAVOh + CUMS (n = 8), CAVOl + CUMS (n = 8), LVO + CUMS (n = 8), and OBI + CAVO + CUMS (n = 8). The CUMS-induced rats were treated for a period of 4 weeks. The other healthy rats were regarded as the control (CTR, n = 8) subjects. The levels of serotonin (5-HT) and dopamine (DA) and their respective metabolites homovanillic acid (HVA) and 5-hydroxyindol acetic acid (5-HIAA) were measured in brain tissue homogenates of CUMS-induced rats using enzyme-linked immunosorbent assay (ELISA). Results CAVO ameliorated depressive-like behaviors (p < 0.05 ). The levels of DA in the CUMS group were lower than those in the CTR and CAVOh groups (** p < 0.01 and * p < 0.05 ). The levels of HVA were lower in the CUMS group than in the CTR, LVO, OBI + CAVOh and CAVOh groups (** p < 0.01 and * p < 0.05 ) and lower in the OBI + CAVOh group than in the CAVOh group (** p < 0.01 ). The levels of 5-HT in the CUMS group were lower than those in the CTR and CAVOh groups (** p < 0.01 ). The levels of 5-HIAA were lower in the CUMS and OBI + CAVOh groups than in the CTR, LVO and CAVOh groups (** p < 0.01 ). Conclusions CAVO can improve depressive-like behaviors concomitant with the regulation of DA and 5-HT metabolism in the brains of CUMS-induced rats.

Construction of Dual-Functional Emulsion Micelle Interface With Ionic and Reactive Groups

Ziyun Li,Qiwei Zheng,Menghan Zhong,Chao Wang,Shengwei Fang,Renfeng Zhou,Min Xia,Yanrong Jia,Juan Zhang,Peng Ye

Internal emulsification emulsions are environmentally friendly and do not emit volatile organic compounds (VOCs). However, their micelle interface, dominated by ionic hydrophilic groups, hinders cross-linking reactions and affects performance. We proposed a dual-functional micellar interface (DFMI) that distributes both ionic and reactive hydrophilic groups, enhancing cross-linking performance without significantly compromising emulsification properties. In this study, a series of sugar-based hyperbranched waterborne polyurethane emulsions were synthesized with 2,2-dimethylolpropionic acid (DMPA) as the ionic hydrophilic moiety. Dopamine (DA) was introduced at varying levels as the reactive hydrophilic moiety for terminal modification. The results showed that monomolecular micelles and vesicular micelles could be formed by symmetric and asymmetric modification of terminal DA. Symmetric modification tended to form unimolecular micelles, while asymmetric modification tended to form vesicular structures. Adhesion tests conducted after cross-linking with gelatin showed that the asymmetrically modified adhesives with seven-layer structured DA exhibited better cross-linking degrees. Adhesion performance and swelling tests confirmed that the asymmetric structure exposed more terminal reactive groups, enhancing the utilization of the modified groups, as indicated by dynamic light scattering (DLS) for emulsion particle size and surface zeta potential. This study successfully prepared hyperbranched polyurethane emulsions with tunable micelle morphology, balancing emulsification ability, and cross-linking degree, supporting the controlled emulsification of hyperbranched polymers and the development of bio-based adhesives.

Implications of N7-hydrogen and C8-keto on the base pairing, mutagenic potential and repair of 8-oxo-2′-deoxy-adenosine: Investigation by nucleotide analogues

Junjie Wu,Mengmeng Zhang,Lulu Song,Yahong Tan,Yosuke Taniguchi,Christopher John Hipolito,Youming Zhang,Yizhen Yin

Oxidative lesions, such as 8-oxo-dG and 8-oxo-dA, are continuously generated from exposure to reactive oxygen species. While 8-oxo-dG has been extensively studied, 8-oxo-dA has not received as much attention until recently. Herein, we report the synthesis of duplex DNAs incorporating dA, 8-oxo-dA, 7-deaza-dA, 8-Br-dA, and 8-Br-7-deaza-dA, which have different substitutions at 7- and 8-position, for the investigation into the implications of N7-hydrogen and C8-keto on the base pairing preference, mutagenic potential and repair of 8-oxo-dA. Base pairing study suggested that the polar N7-hydrogen and C8-keto of 8-oxo-dA, rather than the syn -preference, might be essential for 8-oxo-dA to form a stable base pair with dG. Insertion and extension studies using KF-exo − and human DNA polymerase β indicated that the efficient dGTP insertion opposite 8-oxo-dA and extension past 8-oxo-dA:dG are contingent upon not only the stable base pair with dG, but also the flexibility of the active site in polymerase. The N7-hydrogen in 8-oxo-dA or C7-hydrogen in 7-deaza-dA and 8-Br-7-deaza-dA was suggested to be important for the recognition by hOGG1, although the excision efficiencies of 7-deaza-dA and 8-Br-7-deaza-dA were much lower than 8-oxo-dA. This study provides an insight into the structure-function relationship of 8-oxo-dA by nucleotide analogues.

Nanocellulose composites with enhanced mechanical and flame-retardant properties based on grafting of inorganic organic/multilayer core-shell matter — MSNs-TMSB/DA/TOCNF

Qingshuo Wang,Lanfeng Hui,Yiyi Zhang,Wenwen Guo,Chengfeng Zhu,Mingyue Zhao,Qian Yang,Yu Qu,Zitong Zhang

Flame retardant materials are essential for safety, yet their development is often hindered by trade-offs between efficiency, aging resistance, and mechanical properties. Traditional organic flame retardants are inefficient and degrade over time, while inorganic alternatives, despite their effectiveness, are difficult to integrate into composites. Here we showed the synthesis of a novel inorganic silica-based flame retardant, PDA@MSNs-TMSB, which chemically modified nanocellulose fibrils, enhancing flame retardancy, aging resistance, and toughness without compromising integrity. The results showed that, compared to pure TOCNF, the modified nanocellulose materials (TOCNF-PDA@MSNs-TMSB) exhibited a higher limiting oxygen index (46.5 %), reaching the UL-94 V-0 level (GB) rating with self-extinguishing behavior and no flame propagation. In contrast, pure TOCNF had a limiting oxygen index of only 22 % and burned rapidly upon ignition which did not achieve the UL-94 V-0 level rating. The toughness of the modified TOCNF-PDA@MSNs-TMSB was superior to that of pure TOCNF, representing a 37.5 % increase. Combining powerful tenacity, high flame retardancy, and better aging resistance, the flame retardant nanocellulose material from renewable resource shows great potential for flame retardant applications and emits no toxic byproducts post-combustion.

Tailor-made PEG-DA-CuS nanoparticles enriched in tumor with the aid of retro Diels–Alder reaction triggered by their intrinsic photothermal property

Jie Sheng,Beibei Ma,Qian Yang,Chao Zhang,Zhongying Jiang,Entomack Borrathybay