BIOSENSORS & BIOELECTRONICS
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2014
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IF 6.451
Lu Lu,Jing Cao Si,Zhong Feng Gao,Yu Zhang,Jing Lei Lei,Hong Qun Luo,Nian Bing Li
A dual strategy that combines the adenosine triphosphate (ATP)-dependent enzymatic ligation reaction with self-cleaving DNAzyme-amplified electrochemical detection is employed to construct the biosensor. In this design, the methylene blue-labeled hairpin-structured DNA was self-assembled onto a gold electrode surface to prepare the modified electrode through the interaction of Au–S bond. In the procedure of ATP-dependent ligation reaction, when the specific cofactor ATP was added, the two split oligonucleotide fragments of 8-17 DNAzyme were linked by T4 DNA ligase and then released to hybridize with the labeled hairpin-structured DNA substrate. The linked 8-17 DNAzyme catalyzes the cleavage of the hairpin-structured substrate by the addition of Zn 2+ , causing the methylene blue which contains high electrochemical activity to leave the surface of the gold electrode, therefore generating a dramatic decrease of electrochemical signal. The decrease of peak current was readily measured by square wave voltammetry and a relatively low detection limit (0.05 nM) was obtained with a linear response range from 0.1 to 1000 nM. By taking advantage of the highly specific cofactor dependence of the DNA ligation reaction, the proposed ligation-induced DNAzyme cascades demonstrate ultrahigh selectivity toward the target cofactor ATP. A catalytic and molecular beacons strategy is further adopted to amplify the electrochemical signal detection achieved by cycling and regenerating the 8-17 DNAzyme to realize enzymatic multiple turnover, thus one DNAzyme can catalyze the cleavage of several hairpin-structured substrates, which improves the sensitivity of the newly designed electrochemical sensing system.
研究领域:
生物化学
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电化学
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生物传感器
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纳米技术
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 45
·期 2
·915-922
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IF 3.288
Jianming Zhang,Jia Lin,Tingjin Zheng,Yancheng Jiang,Shimu Luo,Yao Lin,Zhishan Zhang
A novel capacitance immunosensor based on DNAzyme concatemer-amplified signal-generation tags was developed for the sensitive detection of interleukin-6 (IL-6) on an interdigitated micro-comb electrode. To construct such an immunosensor, monoclonal mouse anti-human IL-6 antibodies were covalently conjugated onto a bovine serum albumin (BSA)-coated electrode using the glutaraldehyde cross-linker. Gold nanoparticles heavily functionalized with primer DNA (for the subsequent rolling cycle amplification; RCA) and polyclonal rabbit anti-human IL-6 antibodies were used for the development of a sandwich-type immunoassay on the modified interdigitated electrode in the presence of the target IL-6. Thereafter, the attached primer DNA with the immunocomplex triggered the RCA reaction for the formation of numerous DNAzyme concatemers with the assistance of hemins and G-rich DNA probes. DNAzyme concatemers catalyzed 4-chloro-1-naphthol into insoluble benzo-4-chlorohexadienone and were deposited onto the electrode, thus resulting in the local capacitance variation of the interdigitated micro-comb electrode. The performance and external factors affecting the analytical properties were studied in detail. Under optimum conditions, the capacitance variations of the immunosensor were proportional to the IL-6 concentration in the dynamic range of 1.0–1000 pg mL−1 at a detection limit of 0.68 pg mL−1. Good reproducibility, high specificity and acceptable storage stability were acquired for the analysis of the target IL-6. Moreover, the capacitance immunosensor was utilized to determine IL-6 in human serum specimens and gave well-matched analytical results in reference to the human IL-6 ELISA kit.
研究领域:
生物化学
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分析化学
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纳米技术
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生物传感器
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产品关联
DOI
高影响因子
Advanced Science
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2024
·
IF 14.3
Huiyan Du,Ensheng Xu,Yihan Xu,Qingwang Xue,Hongxia Xu,Jibin Song
The development of theragnostic nanosystems integrating FRET (fluorescence resonance energy transfer) imaging and chemodynamic therapy (CDT) for accurate diagnosis and effective treatment of lung tumors is still a big challenge. Herein, a peptide-assembled 3D DNAzyme motor nanodevice is engineered for a self-powered FRET amplifier profiling human neutrophil elastase (HNE) and self-supplied H 2 O 2 enhancing CDT. The nanodevice is prepared by depositing AuNPs on ZIF-8, in which ZIF-8 co-loaded the lysosomal targeting peptide-modified copper peroxides (PCPs) and hairpins (H1, H2, and H3), AuNPs are co-labeled by DNAzyme-peptide (DP) conjugate and H3. In the tumor micro-environment, HNE driven 3D DNAzyme walker followed by an exponential amplification constructed by a synergistic cross-activation between hybridization chain reaction and DNAzyme, generating a self-powered FRET amplifier. The FRET amplifier specifically measures HNE with a sensitivity of 0.026 pM, and successfully images exogenous HNE in living cells and monitors HNE in mouse models. Moreover, the PCPs can target lysosomes, reducing lysosome escape. The self-supplying H 2 O 2 undertaken by PCPs improves the Cu (II)-catalyzed Fenton-like reaction, effectively causing cell apoptosis to inhibit tumor growth. Significantly, the nanodevice successfully screens inhibitors and discriminates the HNE level in normal and lung cancer tissues, suggesting that the nanodevice provides an effective tool for the diagnosis and treatment of lung tumors.
研究领域:
纳米技术
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癌症诊断与治疗
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2012
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IF 4.555
Ruimin Li,Cen Xiong,Zhiyou Xiao,Liansheng Ling
A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ). Oligonucleotide 5′-GTGGGTAGGGCGGGTTGG-3′ (Oligo-1) formed G-quadruplex structure in the presence of K + , it acted as a horseradish peroxidase (HRP) mimicking DNAzyme when binding hemin and catalyzed the oxidation of colorless ABTS 2− to green ABTS − by H 2 O 2 , which was produced by the reaction of cholesterol and oxygen that catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the color change of ABTS 2− by combining these two reactions. Under the optimum conditions, the absorbance was proportional to the concentration of cholesterol over the range of 1.0–30 μM, with a linear regression equation of A = 0.362 + 0.0256 C ( C : μM, R = 0.998) and a detection limit of 0.10 μM (3 σ /slope). Moreover, the practicability of the assay in the detection of cholesterol in human serum was studied as well.
研究领域:
生物化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
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2023
·
IF 5.4
Xiuyuan Wang,Shusheng Yu,Yuqiu He,Fuan Wang,Xiaoqing Liu
Comprehensive Summary A label-free deoxyribozyme (DNAzyme) biosensor for m 6 A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity-associated protein), an important m 6 A demethylase of ALKBH demethylase family is present, the m 6 A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label-free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2013
·
IF 3.535
Xijing Huang,Yanlin Hao,Hanyin Wu,Qingquan Guo,Liangqia Guo,Jing Wang,Liangshuang Zhong,Tianran Lin,FengFu Fu,Guonan Chen
A simple and sensitive DNAzyme-based colorimetric sensor for detecting Hg 2+ based on T–Hg 2+ -T base pair and magnetic beads (MBs) is proposed. In the presence of Hg 2+ , capture probes (CPs) immobilized on MBs are hybridized with the signal probes (SPs) consisting of G-quadruplex sequences with the help of T–Hg 2+ -T base pairs. The SPs are separated by MBs and used to bind hemin for the formation of catalytically active hemin/G-quadruplex DNAzymes, which catalyzes the H 2 O 2 -mediated oxidation of ABTS to produce a blue-green-colored product. A linear relationship between the absorbance and Hg 2+ concentration is observed over a range of 10–200 nM with a limit of detection 5 nM. Taking advantage of MBs separation/collection process, the sensing system can be regenerated by using EDTA as the chelator.
研究领域:
生物化学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Chang Liu,Siyu Gou,Yanhui Bi,Qi Gao,Juanjuan Sun,Shanjin Hu,Weiwei Guo
A portable, cost-effective and storable DNA-gold nanoparticle (AuNP) hybrid hydrogel film based biosensing system was developed, with AuNPs serving as both the crosslinking units of the film and the signaling units. Using a layer-by-layer assembly method, hydrogel film composed of three-dimensional hydrophilic network of densely packed AuNPs interconnected by responsive DNA structures was constructed onto a glass slide. By programming the sequence of DNA structures, target-responsive hybrid films were constructed. As a proof of concept , the sequence of a substrate DNA which can be identified and cleaved by Pb 2+ -dependent DNAzyme was encoded to construct Pb 2+ -responsive DNA-AuNP hybrid hydrogel film. The high-density packing of AuNPs as signal substances significantly improved the sensitivity of the ultrathin film biosensing system while reduced the cost of expensive DNA materials. A hydrogel film composed of 10 layers of assembled DNA-AuNP structures generated sufficient visual colorimetric signals for Pb 2+ detection, with a detection limit of 2.6 nM. By introducing UO 2 2+ -dependent DNAzyme, the system could be further applied in the sensitive and selective detection of UO 2 2+ , with a detection limit of 10.3 nM. Compared with bulk-sized DNA hydrogel biosensing systems, the DNA-AuNP hydrogel film biosensing system exhibited faster response thanks to the sub-micrometer ultrathin film structures. Moreover, the protection of fragile non-covalently crosslinked DNA films with solid slides also facilitated the portable application and long-term storage of the resulting biosensing system, with 95% of the response signal retained after three months of storage. The DNA-AuNPs hydrogel film biosensing system is highly promising for future rapid on-site detection applications.
研究领域:
生物化学
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纳米生物学
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环境科学
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DOI
高影响因子
FOOD CONTROL
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2024
·
IF 5.6
Wenjie Lu,Xiaoli Dai,Renqing Yang,Zeyan Liu,Haili Chen,Yanfeng Zhang,Xinai Zhang
Pomegranate is very favored but highly susceptible to lead contamination that threatens consumer health, and thereby it is essential to establish efficient modes to monitor lead level in pomegranate. A specific and sensitive electrochemical sensor was developed for assessing lead pollution in pomegranate based on Pb 2+ -dependent DNAzyme as recognition receptor and metal-organic frameworks (MOFs) for signal output. Particularly, the used MOFs exhibited Fenton-like catalytic functions toward methylene blue (MB) as electroactive medium. Integrating with gold nanoparticles (AuNPs), the Fenton-like MOFs showed high sensitivity for electrochemical detection of Pb 2+ in pomegranate. Moreover, benefiting from the specific recognition of DNAzyme to Pb 2+ , the adjustable strategy was achieved, in which the approaching MB led to a higher signal with the absence of Pb 2+ while the detached MB gave rise to a lower signal with the presence of Pb 2+ . With the use of Fenton-like catalytic MOFs driving electrochemical aptasensing interface, this simplified detection pattern was endowed with sensitive assessment of lead pollution level in pomegranate, highlighting its promising potential in evaluating the safety of foodstuffs.
研究领域:
食品安全检测
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环境科学
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生物传感技术
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产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Lianxiao Zhang,Qijie Diao,Bo Li,Xuedan Yang,Teng Wang,Rui Chen,Tianze Jiang,Xia Zhao
Breast cancer is a common malignant tumor in women worldwide, and its high metastasis is the main reason for high mortality. Especially under hypoxic microenvironments, the upregulation of hypoxia-inducible factor 1-alpha (HIF-1α) can activate the transcription factor Twist and promote tumor metastasis through epithelial-mesenchymal transformation (EMT). Here, MnO 2 nanosheets are synthesized with oxidase-like activity using fucoidan as a protective agent, and construct a multifunctional delivery system MnO 2 @DNAzyme-TPZ (MDT) by loading DNAzymes and hypoxia-activated prodrug Tirapazamine (TPZ) for tumor treatment and metastasis inhibition. MDT kills tumors by converting O 2 into reactive oxygen species (ROS) through MnO 2 nanozymes, and inhibits tumor metastasis by selectively silencing the tumor metastasis-related Twist gene through DNAzymes. TPZ enhances tumor killing under hypoxic environments and synergistically inhibits tumor metastasis by reducing the expression of HIF-1α. MDT significantly inhibits tumor growth and metastasis in vivo in an in situ tumor model. The strategy of combining selective gene silencing based on DNAzymes with nanozymes and hypoxia-activated prodrug provides a new approach to anti-tumor metastasis therapy.
研究领域:
肿瘤学
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生物医学工程
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Junhua Chen,Manjia Chen,Hui Tong,Fei Wu,Yizhang Liu,Chengshuai Liu
A fluorescence biosensor was developed for the ultrasensitive detection of the available lead in soil samples by coupling with DNAzyme and hairpin DNA cyclic assembly. The biorecognition between lead and 8-17 DNAzyme will cleave the substrate strands (DNA2) and release the trigger DNA (T), which can be used to initiate the DNA assembly reactions among the hairpins (H1, H2, and H3). The formed Y-shaped sensing scaffold (H1-H2-H3) contains active Mg 2+ -DNAyzmes at three directions. In the presence of Mg 2+ , the BHQ and FAM modified H4 will be cleaved by the Mg 2+ -DNAyzme to generate a high fluorescence signal for lead monitoring. The linear range of the fluorescence biosensor is from 1 pM to 100 nM and the detection limit is 0.2 pM. The biosensor also exhibited high selectivity and the nontarget competing heavy metals did not interfere with the detection results. Compare with the traditional method (DTPA+ICP-MS) for the available lead detection, the relative error (Re) is in the range from −8.3 % to 9.5 %. The results indicated that our constructed fluorescence biosensor is robust, accurate, and reliable, and can be applied directly to the detection of the available lead in soil samples without complex extraction steps.
研究领域:
环境科学
·
分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Guo Liu,Shan Huang,Xiaochen Liu,Wanzhen Chen,Xin Ma,Shuang Cao,Liping Wang,Lanlan Chen,Huanghao Yang
Transmembrane signal transduction is of profound significance in many biological processes. The dimerization of cell-surface receptors is one prominent mechanism by which signals are transmitted across the membrane and trigger intracellular cascade amplification reactions. Recreating such processes in artificial systems has potential applications in sensing, drug delivery, bioengineering, and providing a new route for a deep understanding of fundamental biological processes. However, it remains a challenge to design artificial signal transduction systems working by the receptor dimerization mechanism in a predictable and smart manner. Here, benefitting from DNA with features of programmability, controllability, and flexible design, we use DNA as a building material to construct an artificial system mimicking dimerization of receptors for signal transduction and cascade amplification. DNA-based membrane-spanning receptor analogues are designed to recognize external signals, which drives two receptors into close spatial proximity to activate DNAzymes inside the cell-mimicking system. The activation of the DNAzyme initiates the catalyzed cleavage of encapsulated substrates and leads to the release of fluorescent second messengers for signal amplification. Such an artificial signal transduction system extends the range of biomimetic DNA-based signaling systems, providing a new avenue to study natural cell signaling processes and artificially regulate biological processes.
研究领域:
合成生物学
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分子生物学
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-7
·
IF 5.479
Wang Yulong,Wang Limin,Zhang Cunzheng,Liu Fengquan
A DNAzyme-based catalytic and stem-loop based amplification scheme is used in a Cu(II)-specific lateral flow assay (LFA). Three test lines with given cut-off value on the test strip are set as the signal indicating zone for semiquantitative analysis by the number of red color bands that appear after lateral flow. The colored bands are generated by accumulation of gold nanoparticles. Four detection ranges can be visualied: (a) 0–2 ng·mL −1 (= negative); 2–50 ng·mL −1 ; 50–200 ng·mL −1 and > 200 ng·mL −1 of Cu(II) (= positive). The visual detection limit is thus considered as being 2 ng·mL −1 which is much lower than the U.S. EPA limit in drinking water (1.25 μg·mL −1 ). The highly specific DNAzyme, the strong multiple-turnover catalytic target recycling property and highly efficient amplification strategy warrant the high specificity, sensitivity and rapidity of this LFA. Conceivbly, this detecton scheme can be extended to other metal ions by proper choice of the ion-specific DNAzyme.
Schematic presentation of a semiquantitative lateral flow test strip for Cu 2+ analysis by three visual cut-off value test lines using catalytic and stem-loop based signal amplification strategy.
研究领域:
生物化学
·
环境科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）