JOURNAL OF PLANT GROWTH REGULATION
·
2019
·Vol. 39
·期 1
·358-369
·
IF 2.179
Cui Dandan,Mo Yuncai,Zeng Lingjie,Feng Kai,Feng Xiaoyun,Huang Jialing,He Mengling,Zhang Xiaoyuan,Teng Xifeng
The response of Dendrobium officinale Kimura et Migo ( D. officinale ) to continuous UV-B irradiation at different carbon to nitrogen ratios (C/N ratios) was investigated. Seedlings grown for 60 days were incubated under aseptic conditions with UV-B irradiation (15.6 µW cm −2 ) at different C/N ratios: control group (CK; C/N 30 without UV-B), UV-B + CK (C/N 30 with UV-B irradiation, similarly hereafter), UV-B + C/N 120, UV-B + C/N 60, UV-B + C/N 15, UV-B + C/N 10, UV-B + C/N 7.5. Growth parameters (the defoliation rate and the sprout number), photosynthetic pigments (carotenoids, chlorophyll a and chlorophyll b), total polysaccharides, total alkaloids, and activities of antioxidant enzymes were determined following 4, 8, 12, and 16 days of continuous UV-B exposure. Results indicated that UV-B irradiation increased the defoliation rate and the content of carotenoids, total polysaccharides and total alkaloids, as well as the activities of antioxidant enzymes. Conversely, UV-B irradiation reduced the sprout number and chlorophyll content in D. officinale . Compared with UV-B + CK, lower C/N ratio treatments (UV-B + C/N 15, UV-B + C/N 10 and UV-B + C/N 7.5) enhanced the defoliation rate and sprout number, but decreased antioxidant enzyme activities and total polysaccharide content during the whole period, and reduced total alkaloid content after 4 days of UV-B exposure. Following initial UV-B irradiation, lower C/N ratios increased the contents of carotenoid and chlorophyll b, while after 8 days, a reversal in carotenoid content was observed, and after 12 days, a reversal in chlorophyll b content was observed. Optimizing the C/N ratio (C/N 60) resulted in lower defoliation rate, higher photosynthetic pigments and total polysaccharides, and increased activities of antioxidant enzymes, whereas no significant change in sprout number and total alkaloid content was recorded under long-term UV-B irradiation. Furthermore, the UV-B + C/N 120 treatment negatively affected D. officinale in terms of an increased defoliation rate and reduced sprout number, photosynthetic pigments, and total alkaloids. Therefore, results suggested that an appropriate C/N ratio (C/N 60) could ameliorate the adverse effects of continuous UV-B irradiation on D. officinale .
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Food Bioscience
·
2025
·
IF 5.9
Shu He,Zhengli Lu,Rurui Li,Chun Chen,Xiong Fu,Kin Weng Kong,Amin Ismail,Xuechun Zhang,Zhenxing Wang
This study evaluated the chemical composition and bioactivity of nine Dendrobium officinale from three cultivation modes (greenhouse, GC; understory, UC; and simulated wild, SWC) across China. Results demonstrated that UC-cultivated samples exhibited significantly higher flavonoid levels, antioxidant capacity, and α-glucosidase inhibition activity than those under GC/SWC modes. Using GC-IMS and UHPLC-TOF-MS, metabolomic profiling identified 27 volatile and 15 non-volatile biomarkers between different cultivation modes. Further KEGG analysis revealed the key signaling pathways of differential metabolites under different cultivation modes, including aminobenzoate degradation, phenylpropanoid biosynthesis, and phenylalanine metabolism. Overall, the cultivation mode significantly influences the chemical composition and biological activity of Dendrobium officinale , with the UC mode being relatively preferable. However, factors such as geographical distribution also play an important role. These findings provide actionable insights for quality control, cultivation optimization, and functional food/pharmaceutical applications of Dendrobium officinale .
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Chemistry Central Journal
·
2018
·Vol. 12
·期 1
·1-10
·
IF 2.284
Xing Shangping,Zhang Xiaofeng,Ke Hannu,Lin Ji,Huang Yuechun,Wei Gang
Background Dendrobium officinale as a precious traditional Chinese herb is widely used in medicines and health supplements. Thus the extraction, purification and biological activities of polysaccharides from the stem of Dendrobium officinale have significant meaning on theory and application value. Methods The crude Dendrobium officinale polysaccharide (DOP) was obtained by hot water extraction- ethanol precipitation method, and four new polysaccharide fractions (DOP-40, DOP-50, DOP-60, and DOP-70) were further obtained from the crude DOP by fractional precipitation with ethanol method, then four fractions were further purified by Toyopearl-H65F gel resin. The molecular weight and monosaccharide composition of four purified fractions were determined by high performance anion exchange chromatography and high performance liquid chromatography. The antioxidant activities of them were evaluated by the reducing power assay, and the superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl free radicals scavenging assays, respectively. Finally, the anticancer activities of them were investigated via the MTT assay and the western blot analysis using HepG2 cells. Results Among these four purified fractions were mainly composed of d -mannose and d -glucose with different molar ratios, and their average molecular weights were 999, 657, 243 and 50.3 kDa, respectively. What’s more, DOP-70 always exhibited the strongest antioxidant and anticancer activities, while DOP-40 and DOP-60 showed very close antioxidant and anticancer activities which were better than that of DOP-50. The western blotting analysis also showed that DOP-40, DOP-60, and DOP-70 induced apoptosis in HepG2 human liver cancer cells through the Bcl-2 and Bax-dependent pathway. Conclusions Fractional precipitation with ethanol could successfully apply to extract four new polysaccharide fractions from Dendrobium officinale stems, and the polysaccharide fractions possessed efficient antioxidant and anticancer activities, especially DOP-70.
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PLANT CELL TISSUE AND ORGAN CULTURE
·
2024
·Vol. 156
·期 2
·1-11
·
IF 2.3
Zhang Gaowei,Luo Liping,Tian Kaili,Ning Fangjian,Wu Dong,Sun Qifang,Yu Wenjie,Yi Shengxiang,Hao Yingbin
Dendrobium officinale is a perennial herb with medicinal and edible qualities. The growth and nutritional properties of this plant are significantly affected by the light quality. However, the impact of varying light quality on the phytochemical characteristics of tissue-cultured seedlings of D. officinale is still unknown. In this study, tissue-cultured seedlings of D. officinale were grown for ninety days under six LED lights, including white light (W), blue LED (B), red LED (R), and three composite light environments with varying quantities of red and blue light: 1:2 (R1B2), 1:1 (R1B1), and 2:1 (R2B1). The growth status and physiological signs of the seedlings were analyzed and we used high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-Q/TOF-MS), and pathway enrichment analysis to thoroughly examine the metabolomic changes, with a focus on phenolic compounds. Our results indicated that R2B1 illumination led to the best growth of D. officinale seedlings, with maximal energy absorption, and phenolic contents. In conclusion, R2B1 was considered the most profitable for tissue-culture production of D. officinale .
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Frontiers in Plant Science
·
2022
·
IF 6.627
Song Cheng,Zhang Yunpeng,Manzoor Muhammad Aamir,Li Guohui
Jasmonate (JA) signaling plays a pivotal role in plant stress responses and secondary metabolism. Many studies have demonstrated that JA effectively induce the expressions of alkaloid biosynthetic genes in various plants, which rendered to the accumulation of alkaloid to counteract stresses. Despite the multiple roles of JA in the regulation of plant growth and different stresses, less studied involved in the regulatory role of JA in Dendrobium officinale alkaloids. A strategy for the rapid identification of alkaloid and the intermediates of D. officinale was established based on a solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry method. By using SPE-LC-MS/MS method, the potential compounds were tentatively identified by aligning the accurate molecular weight with the METLIN and Dictionary of Natural Products databases. The chemical structures and main characteristic fragments of the potential compounds were further confirmed by retrieving the multistage mass spectra from the MassBank and METLIN databases. The Mass Frontier software was used to speculate the fragmentation pathway of the identified compounds. Seven alkaloids were separated and identified from D. officinale, which were mainly classified into five types (tropane alkaloids, tetrahydroisoquinoline alkaloids, quinolizidine alkaloids, piperidine alkaloids, and spermidine alkaloids). Besides the alkaloids, forty-nine chemical substances, including guanidines, nucleotides, dipeptides, sphingolipids and nitrogen-containing glucosides, were concurrently identified. These findings gives the composition of chemicals currently found in D. officinale, which could provide the scientific method for the identification of alkaloids in other Dendrobium plants.
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ACS Omega
·
2020
·
IF 2.87
Ming Hu,Xue-Qiong Yang,Cui-Fang Wang,Tong-De Zhao,Dai-Li Wang,Ya-Bin Yang,Zhong-Tao Ding
Six new polyketides named paraverrucsins A–F (1–6) with oxabicyclic and dioxatricyclic skeletons, together with eight known metabolites (7–14), were discovered and isolated from the fermentation medium of Paraphaeosphaeria verruculosa. Paraverrucsin A–C possessed a novel decarboxylated skeleton compared with that of trichocladinols. Their structures were elucidated by extensive spectral analysis and DP4+ calculations. Paraverrucsins B/C and D/E were isolated as a mixture for the mutarotation occurred at C-2. Paraverrucsins B/C, D/E, F/trichocladinol B, 8, and 9 displayed antifeedant activities against silkworm larvae, with antifeedant index percentages ranging from 62.5 to 93.0%, at a concentration of 50 μg/cm2. Among them, Paraverrucsins B/C and 9 had EC50 values at 13.9 and 18.2 μg/cm2. Most compounds showed antifungal activities against phytopathogenic fungi with minimum inhibitory concentration (MIC) values of 16–64 μg/mL. Coculture of P. verruculosa and host plant Dendrobium officinale leads to the enhancement of antifeedant and antiphytopathogenic activities. Compounds 1, 2/3, 4/5, 6/14 were tested for cytotoxicity against five human carcinoma cell lines, HL-60, A549, MCF-7, SW480, and SMMC-7721, while they exhibited selected cytotoxicity against SW480 with inhibition ratios of 32–38% at a concentration of 40 μM.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Bingquan Chu,Weiyi Jin,Cheng Feng,Xiaohua Cui,Lin Gan,Guanghua He,Gongnian Xiao,Genxiang Mao
The bioactive molecules of Dendrobium officinale , an edible medicinal plant native to China, comprise mainly polysaccharides, which exhibit diverse physiological activities. However, the rigid cell wall structure of D. officinale hinders their efficient release. Steam explosion (SE) is an effective pretreatment technology for disrupting the cell walls of raw materials. In this study, the effects of SE at severity factors (SF) of 3.50 and 3.91 on the yield of D. officinale polysaccharides were evaluated. Additionally, the structural features, chemical composition, and antioxidative activities of polysaccharides from SE-treated D. officinale (SEDOPs) before and after in vitro digestion were analyzed. Compared with the untreated group (DOP), the SE-treated samples exhibited enhanced polysaccharide release rates. Additionally, SEDOP 3.50 was significantly purer and had a higher uronic acid content than that of DOP. SE treatment also reduced molecular weights of the polysaccharides. Mannose and glucose were identified as the predominant monosaccharides, with SE altering their molar ratios. Structurally, the SEDOPs developed porous architectures while their primary structures remained preserved. Their triple-helical conformations were disrupted following intestinal digestion. Furthermore, SE treatment enhanced their antioxidative capacities, with SEDOP 3.50 consistently exhibiting the highest uronic acid content and strongest antioxidative activity throughout the digestion process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yonggan Sun,Shanshan Zhang,Huijun He,Haihong Chen,Qixing Nie,Song Li,Jiaobo Cheng,Baojie Zhang,Zhitian Zheng,Shijie Pan,Ping Huang,Lu Lian,Jielun Hu,Shaoping Nie
Dietary fiber is crucial for human health mainly due to its impact on gut microbiota structure and metabolites. This study aimed to investigate the impact of Dendrobium officinale polysaccharides (DOP) and two common fibers (β-glucan and inulin) on the gut microbiome structure and metabolic profile in vitro . Fecal samples were obtained from 30 healthy volunteers, which were then individually subjected to fermentation with each type of fiber. The results revealed that all fibers were efficiently degraded by gut microbiota, with DOP exhibiting a slower fermentation rate compared to β-glucan and inulin . The fermentation of all fibers led to a significant increase in the production of short-chain fatty acids (SCFAs) and a reduction in branched-chain fatty acids (BCFAs), sulfides, phenols, and indole. Moreover, the abundance of unclassified Enterobacteriaceae , which was positively correlated with sulfide, phenols, and indole levels, was significantly reduced by all fibers. Additionally, DOP specifically promoted the growth of Parabacteroides , while β-glucan and inulin promoted the growth of Bifidobacterium and Faecalibacterium . Taken together, these findings enhance our understanding of the role of DOP, β-glucan, and inulin in modulating gut microbiota and metabolites, where the fermentation with fecal bacteria from different volunteers could provide valuable insights for personalized therapeutic approaches.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Zijun Li,Siyang Wang,Yanhui Wang,Liyan Shen,Shiyu Hu,Zhouguang Wang,Ruiqin Zhang,Zhen Li
DOP-W-1 was identified as a branched α-glucan from Dendrobium officinale . • DOP-W-1 alleviated DSS-induced colitis and preserved mucosal barrier integrity. • Gut microbiota depletion largely abolished the anti-colitic effect of DOP-W-1. • Fecal microbiota from DOP-W-1-treated mice transferred colitis protection. • DOP-W-1 restored SCFAs and corrected aromatic amino acid-related metabolism. A homogeneous polysaccharide, DOP-W-1, was isolated from Dendrobium officinale and identified as a low-molecular-weight branched α-glucan with a molecular weight of 9.76 kDa, mainly consisting of →4)-α-D-Glcp-(1 → and →4,6)-α-D-Glcp-(1 → residues. This study aimed to determine whether DOP-W-1 protects against dextran sulfate sodium (DSS)-induced colitis through gut microbiota-mediated mechanisms. In DSS-induced colitic mice, DOP-W-1 markedly alleviated disease severity, as shown by reduced body weight loss and disease activity index, restored colon length, improved histopathological injury, preserved mucus barrier integrity, decreased intestinal permeability, and rebalanced inflammatory cytokines. 16S rRNA sequencing revealed that DOP-W-1 selectively reshaped the gut microbiota, characterized by enrichment of Akkermansia and suppression of DSS-associated taxa, including Turicibacter and Alistipes . Importantly, antibiotic-mediated microbiota depletion largely abolished the protective effects of DOP-W-1, whereas fecal microbiota transplantation from DSS + DOP-W-1-treated donor mice reproduced the anti-colitic phenotype, demonstrating that gut microbiota is essential for its protective activity. Functional analysis further showed that DOP-W-1 restored microbial carbohydrate-active enzyme profiles associated with α-glucan utilization. Untargeted metabolomics and short-chain fatty acid analysis indicated that DOP-W-1 corrected aromatic amino acid-related metabolic disturbances, restored D-glucaro-1,4-lactone, and recovered short-chain fatty acid production. Collectively, these findings suggest that DOP-W-1 acts as a microbiota-targeted α-glucan that alleviates experimental colitis by restoring microbial carbohydrate metabolism, metabolic homeostasis, and mucosal barrier function. Download: Download high-res image (164KB) Download: Download full-size image
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 10
·2100772
·
IF 6.575
Hong Zheng,Hui Ji,Kai Fan,Hangying Xu,Yinli Huang,Yafei Zheng,Qingqing Xu,Chen Li,Liangcai Zhao,Yuping Li,Hongchang Gao
Scope Obesity is becoming a major public health problem due to excess dietary fat intake. Dendrobium officinale ( D. officinale ) is a medicine food homology plant and exerts multiple health-promoting effects. However, its antiobesity effects and the potential mechanisms remain unclear. Methods and results High-fat diet (HFD)-fed mice are administered D. officinale dietary fiber (DODF) daily by gavage for 11 weeks. The results show that treatment with DODF alleviates obesity, liver steatosis, inflammation, and oxidant stress in HFD-induced obese mice. Improved glucose homeostasis in obese mice after DODF treatment is achieved by enhancing insulin pathway and hepatic glycogen synthesis. DODF restructures the gut microbiota in obese mice by decreasing the relative abundance of Bilophila and increasing the relative abundances of Akkermansia, Bifidobacterium , and Muribaculum . Also, DODF reshapes the metabolic phenotype of obese mice as indicated by up-regulating energy metabolism, increasing acetate and taurine, and reducing serum low density/very low density lipoproteins (LDL/VLDL). These beneficial effects are partly transferred by FMT, implying the gut microbiota as a target for the protective effect of DODF on obesity-related symptoms. Conclusion The results suggest that DODF can be used as a novel prebiotics to maintain the gut microbial homeostasis and improve metabolic health, preventing obesity and related metabolic syndrome.
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Pharmaceutics
·
2025
·Vol. 17
·期 1
·97
·
IF 5.5
Shengchang Tao,Huan Wang,Qiufeng Ji,Yushan Yang,Gang Wei,Ruiming Li,Benjie Zhou
Background: The mechanism ofDendrobium officinalepolysaccharide-based nanocarriers in enhancing photodynamic immunotherapy in colorectal cancer (CRC) remains poorly understood.Methods: The effects of TPA-3BCP-loaded cholesteryl hemisuccinate–Dendrobium officinalepolysaccharide nanoparticles (DOP@3BCP NPs) and their potential molecular mechanism of action in a tumor-bearing mouse model of CRC were investigated using non-targeted metabolomics and transcriptomics. Meanwhile, a histopathological analysis (H&E staining, Ki67 staining, and TUNEL assay) and a qRT-PCR analysis revealed the antitumor effects of DOP@3BCP NPs with and without light activation.Results: Through metabolomics and transcriptomics analysis, we found an alteration in the metabolome and functional pathways in the examined tumor tissues. The metabolic analysis showed 69 and 60 differentially expressed metabolites (DEMs) in positive- and negative-ion modes, respectively, in the treated samples compared to the Control samples. The transcriptomics analysis showed that 1352 genes were differentially expressed among the three groups. The differentially regulated functional pathways were primally related to the antitumor immune response. The results of the pathological histology assay and qRT-PCR analysis verified the findings of the integrated metabolomics and transcriptomics analysis.Conclusions: Overall, our findings elucidate the potential antitumor mechanisms of theD. officinalepolysaccharide-based nanocarrier in enhancing photodynamic immunotherapy in CRC.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Hui Duan,Qun Yu,Yang Ni,Jinwei Li,Leilei Yu,Xiaowei Yan,Liuping Fan
The importance of synergy has been underscored in recent medical research for augmenting the efficacy of therapeutic interventions, targeting multiple biological pathways simultaneously. Our prior research elucidated that Dendrobium officinale polysaccharide (DOP) has the potential to prolong the lifespan of Caenorhabditis elegans ( C. elegans ) via regulating gut microbiota. Concurrently, spermidine (Spd), as a mimicking caloric restriction, facilitates autophagy and exerts a pronounced anti-aging effect. To enhance the anti-aging capabilities of DOP, we conducted a comprehensive study examining the combined effects of DOP and Spd in C. elegans , incorporating metabolomics analysis to investigate the underlying mechanisms. A combination of 250 mg/L DOP and 29.0 mg/L Spd yielded the most favorable outcomes in lifespan extension, evidencing a synergistic effect with a combination index (CI) of 0.65. In oxidative and heat stress tolerance assays, the observed CIs were 0.50 and 0.33, respectively. Metabolomic analysis highlighted significant alterations in metabolites related to lipid, nucleotide and energy metabolism, notably regulating glycerol 3-phosphate, linoleoyl glycerol, docosapentaenoic acid and β-nicotinamide mononucleotide, nicotinamide adenine dinucleotide. The effects of DS on lipid metabolism were further validated using Oil Red O staining and triglyceride level in C. elegans . The results indicated that DS may primarily be via modulating lipid metabolism. To further confirm these findings, a high-fat diet-induced mouse model was employed. Consequently, it can be inferred that the synergistic anti-aging impact of DOP and Spd is likely mediated primarily through alterations in lipid metabolic processes.
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