Journal of Cleaner Production
·
2023
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IF 11.1
Bingjie Huo,Jingxue Wang,Zichen Wang,Xiaowen Zhang,Jingwei Yang,Yinglong Wang,Jianguang Qi,Wei Ma,Fanqing Meng
E-MoS 2 /PVDF piezoelectric microcapsules with superior piezocatalytic performance were synthesized by coupling of expanded MoS 2 (E-MoS 2 ) and polyvinylidene fluoride (PVDF). The bubble-driven piezocatalysis process was proposed for the first time, using as-prepared E-MoS 2 /PVDF microcapsules as piezocatalyst for the degradation of antibiotics. Results showed that the degradation efficiency of tetracycline (TC), chlortetracycline (CH), and ofloxacin (OFL) in the E-MoS 2 /PVDF microcapsules solution was 91.88%, 85.48% and 96.84% in 60 min, respectively. After 5 times of recycling, the degradation rate of TC still reaches more than 73%. Importantly, the energy consumption of this bubble-driven piezocatalysis process (76.00 kwh −3 order −1 ) was only 6.43% of ultrasonic processes. In addition, the study of the degradation mechanism found that the bubble-driven stimulated the generation of more 1 O 2 , O 2 − while increasing the content of dissolved oxygen in the water, which promoted the piezocatalytic degradation. The possible degradation paths were obtained, and the toxicity migration during the degradation of tetracycline was evaluated. The bubble-driven method provides a direction for reducing energy consumption and supplementing the oxygen consumption of degradation in wastewater pollutant treatment.
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2018
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IF 4.946
Chun Wang,Keyu Xing,Ganggang Zhang,Meifang Yuan,Shaolan Xu,Daofeng Liu,Wenyao Chen,Juan Peng,Song Hu,Wei-Hua Lai
Escherichia coli O157:H7 ( E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended block acted as a scaffold to prepare fluorescent Ag nanoclusters (AgNCs). The anchoring block contained Poly A, which bound with the surface of gold nanoparticles to quench the fluorescence of AgNCs. An interesting ELISA approach for detecting E. coli O157:H7 was established via fluorescent quenching of DNA-stabilized AgNCs by using a sandwich complex. The changes in fluorescence intensity of AgNCs were used to quantitatively detect E. coli O157:H7. The sensitivity for detecting E. coli O157:H7 reached 1.905 × 10 3 CFU/mL with a good linear range. Compared with conventional ELISA, the sensitivity of this technique increased by 30-fold. Moreover, this method demonstrated specificity and reproducibility and could be used in food samples.
研究领域:
生物技术
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分析化学
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微生物学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CONNECTIVE TISSUE RESEARCH
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2023
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IF 2.9
Gao-Feng Zhao,Le-Bin Huang
Aims Osteoarthritis (OA) is a chronic inflammatory disease, characterized by degradation of extracellular matrix, apoptosis of chondrocytes and inflammation in articular cartilage. Zinc finger E-box binding homebox 2 (ZEB2), a transcription repressor, has been demonstrated with anti-inflammatory role in some cells. The analysis from GEO data reveals that ZEB2 expression is upregulated in articular cartilage of OA patients and experimental OA rodents. This study aims to confirm the function of ZEB2 in OA process.Material and methods The experimental OA was induced by anterior cruciate ligament transaction (ACLT) in rats, and the adenovirus loaded with ZEB2 coding sequence was intra-articularly injected into rats (1 × 10 PFU). The primary articular chondrocytes were stimulated by interleukin-1β (IL-1β) (10 ng/ml) to mimic the osteoarthritic injury, and transfected with the adenovirus carrying ZEB2 coding or silencing sequence. The apoptosis, content of extracellular matrix, inflammation, and the activity of NFκB signaling in chondrocytes and cartilage were determined.Results ZEB2 was highly expressed in osteoarthritic cartilage tissues and IL-1β-treated chondrocytes. The overexpression of ZEB2 restrained ACLT- or IL-1β administration-induced apoptosis, matrix degradation and inflammation in vivo or in vitro, evidenced by changed levels of cleaved caspase-3/PARP, collagen-II, aggrecan, matrix metalloproteinase 3/13, tumor necrosis factor-α and interleukin-6. Additionally, the phosphorylation of NFκB p65, IκBα and IKKα/β, and the nuclear translocation of p65 was blocked by ZEB2, implying inactivation of this signaling.Conclusions ZEB2 mitigated osteoarthritic symptoms in rats and chondrocytes, and NFκB signaling might be involved. These findings may provide novel insights for clinical treatment of OA.
研究领域:
骨科
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分子生物学
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细胞生物学
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病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Huihui Gao,Xiankun Wu,Yaowen Hu,Mang Wu,Wei Liu,Zhongkai Wang
Although tremendous efforts have been made to convert woody oils into valuable chemicals, most of the known protocols still require multi-step procedures and rigorous conditions. Herein, a highly efficient method for converting woody oils into long-chain unsaturated dicarboxylic acid was reported. Such a strategy holds promises for opening new routes for utilizing woody oils. Firstly, self-metathesis of fatty acids from Rubber seed oil (RSO), Sapium sebiferum oil (SSO) and High oleic Canola oil (HOCO) were optimized. Pure E -octadec-9-enedioic acid monomer (C18) was obtained, and confirmed by 1 H NMR and FTIR spectra. The prepared dicarboxylic acid was then experienced condensation polymerization with 1,6-hexamethylenediamine. The chemical structures of the obtained polyamides were investigated by FTIR and 13 C NMR spectra. DSC , TGA, DMA, and tensile testing was used to explored their thermal and mechanical properties. Interestingly, due to the existence of broad melting and glass transition, the prepared polyamides possess multiple-shape memory properties.
研究领域:
化学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Ziyu Gan,Jun Wang
Plants exhibit rapid responses to biotic and abiotic stresses by releasing a range of volatile organic compounds (VOCs). Monitoring changes in these VOCs holds the potential for the early detection of plant diseases. This study proposes a method for identifying late blight in potatoes based on the detection of (E) -2-hexenal, one of the major VOC markers released during plant infection by Phytophthora infestans . By combining the Michael addition reaction with cysteine-mediated etching of aggregation-induced emission gold nanoclusters (Au NCs), we have developed a portable hydrogel kit for on-site detection of (E) -2-hexenal. The Michael addition reaction between (E) -2-hexenal and cysteine effectively alleviates the etching of cysteine-mediated Au NCs, leading to a distinct fluorescence color change in the Au NCs, enabling a detection limit of 0.61 ppm. Utilizing the superior loading and diffusion characteristics of the three-dimensional structure of agarose hydrogel, our sensor demonstrated exceptional performance in terms of sensitivity, selectivity, reaction time, and ease of use. Moreover, quantitative measurement of (E) -2-hexenal was made easier by using ImageJ software to transform fluorescent images from the hydrogel kit into digital data. Such method was effectively used for the early detection of potato late blight. This study presents a low-cost, portable fluorescent analytical tool, offering a new avenue for on-site detection of plant diseases.
研究领域:
植物病理学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2020
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IF 3.616
Jiahui Sun,Jing Li,Qi Liu,Min Jiang,Mengjia Yang,Siwen Zhan,Tong Qiu,Kaiyong He,Xueqiong Zhang
This study aimed to develop, evaluate, and optimize the mPEG-PLA/vitamin E-TPGS mixed micelle drug delivery system to encapsulate celecoxib (CXB) and honokiol (HNK) for intravenous treatment of breast cancer. To this end, we formulated CXB-loaded mPEG-PLA/vitamin E-TPGS (PV-CXB) and HNK-loaded mPEG-PLA/vitamin E-TPGS (PV-HNK) mixed micelles and analyzed their characteristics. The 4T1 cell line was used for cytotoxicity determination and cellular uptake experiments, and for establishing a 4T1-bearing mouse model for histopathology, immunofluorescence, terminal deoxynucleotidyl transferase-mediated nick end labeling, and Western blot analysis . The synergistic effects of PV-CXB and PV-HNK combination therapy were investigated in vitro and in vivo using the coefficient of drug interaction values. The mean size of PV-CXB and PV-HNK prepared with optimal formulation was approximately 50 nm , with a spherical shape. PV-CXB and PV-HNK combination therapy exhibited cytotoxicity in 4T1 cells in vitro. However, the toxicity of PV-CXB and PV-HNK combination therapy was not apparent in normal tissues (heart, liver, spleen, lung, and kidney) in vivo and reduced the expression of collagen fibers in tumor tissues. Moreover, the combination therapy reduced the expression of tumor growth biomarkers (Foxp3, CD4, Gr-1, CD11b, CD31, Ki67, FoxM1, and VEGF). In addition, the tumor cell apoptosis rate reached 45.71 ± 0.62%. The combined treatment with PV-CXB and PV-HNK showed synergistic effect both in vitro and in vivo. Thus, the PV-CXB and PV-HNK drug delivery system could be used as a potential combination therapy for breast cancer .
研究领域:
药剂学
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肿瘤学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2016
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IF 3.994
Jing Wang,Peng Xue,Jiahua Zhou,Lin Li,Lu Xu,Yongjun Wang
To achieve optimal therapeutic index of docetaxel , we conjugated docetaxel (DTX) with vitamin E (VE) by ester bond with disulfide bond or thioether bond as spacer to produce DTX-ss-VE or DTX-s-VE. The two prodrugs were successfully loaded into liposomes . The physicochemical characterization and in vitro release profiles of the prodrug loaded liposomes were investigated. MTT assays showed that the cytotoxicity of DTX-ss-VE loaded liposomes on PC3 (IC 50 = 27.5 nM) and A549 cells (IC 50 = 63.4 nM) was comparable with the cytotoxicity of DTX-s-VE loaded liposomes (IC 50 = 99.2 and 159.5 nM, respectively). The pharmacokinetic studies demonstrated that DTX-ss-VE and DTX-s-VE loaded liposomes exhibited an extended DTX half-life (3.6 ± 1.2 and 10.0 ± 3.0 h, respectively) as compared to DTX solutions (2.1 ± 1.5 h) and an increased AUC (30487.3 ± 3791.6 and 20922.1 ± 5633.3 ng/L × h, respectively) compared with DTX solutions (1779.3 ± 226.6 ng/L × h). Finally, the in vivo anti-tumor studies showed that DTX-ss-VE loaded liposomes possessed similar antitumor activity compared with DTX solutions. Unexpectedly, not any tumor inhibition effect was observed in DTX-s-VE loaded liposomes group. In a conclusion, our studies suggested that simplex favorable properties of the anticancer prodrug can not ensure available good therapeutic index and the rational design of prodrug needs a comprehensive understanding of the in vitro and in vivo behavior of the prodrug.
研究领域:
药理学
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肿瘤学
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药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Yuting Rong,Jialing Xie,Haibo Yuan,Lilei Wang,Fuqiao Liu,Yuliang Deng,Yongwen Jiang,Yanqin Yang
Storage time is one of the important factors affecting the aroma quality of Pu-erh tea. In this study, the dynamic changes of volatile profiles of Pu-erh teas stored for different years were investigated by combining gas chromatography electronic nose (GC-E-Nose), gas chromatography-mass spectrometry (GC–MS), and gas chromatography-ion mobility spectrometry (GC-IMS). GC-E-Nose combined with partial least squares-discriminant analysis (PLS-DA) realized the rapid discrimination of Pu-erh tea with different storage time (R 2 Y = 0.992, Q 2 = 0.968). There were 43 and 91 volatile compounds identified by GC–MS and GC-IMS, respectively. A satisfactory discrimination (R 2 Y = 0.991, and Q 2 = 0.966) was achieved by using PLS-DA based on the volatile fingerprints of GC-IMS. Moreover, according to the multivariate analysis of VIP > 1.2 and univariate analysis of p < 0.05, 9 volatile components such as linalool and (E)-2-hexenal were selected as key variables to distinguish Pu-erh teas with different storage years. The results provide theoretical support for the quality control of Pu-erh tea.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2020
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IF 4.65
Xinjie Yun,Qifan Wu,Lei Feng,Jiancang Shen,Jian Chen,Paul K. Chu,Lizhe Liu,Xinglong Wu
Magnetic particles can be combined with carbon materials to prepare core-shell microwave absorbers and the proper core/shell weight ratio yields the optimal absorption effects. However, few studies have focused on the influence of core surface modification on the microwave absorption performance. In this work, e-Fe 3 O 4 @C composite microspheres are synthesized by surface etching of core Fe 3 O 4 microspheres in HCl followed by polymerization/carbonization. Core surface modification improves the microwave absorption capability of the e-Fe 3 O 4 @C microsphere films. The minimal reflection loss (RL min ) is −54.38 dB at 12.4 GHz for a film thickness of 2.6 mm and the maximum effective bandwidth is extended to 4.1 GHz (3 mm). Etching of the Fe 3 O 4 core surface increases the interfacial contact area consequently enhancing interface polarization and defects created by surface etching produce additional dipole polarization to further increase the dielectric loss. The underlying mechanism is investigated and described. The results reveal a novel and effective strategy to enhance the properties of core-shell microwave absorption materials.
研究领域:
材料科学
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电磁学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
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IF 6.2
Wenjing Li,Ling Xiao,Juan Qi,Yuanyuan Zhang,Zhu Huang,Liangliang Zhu
Inhibition of gut β-glucuronidase (GUS) protects the gastrointestinal tract by reducing local exposure to toxic aglycones from inactive glucuronides. Development of highly safe GUS inhibitors has attracted extensive attentions. The current study demonstrates that natural and synthetic vitamin E (VE) analogues all display potent inhibition toward GUS. dl-α-tocopherol acts as the most potent inhibitor with the Ki value of 0.14 μM, while the other analogues have Ki values below 15.1 μM. Docking analysis indicates that the amino residues in the active pocket of GUS form various hydrophobic interactions with both the chromanol ring and side chain of VE, as well as hydrogen bonds with the hydroxyl group. It is predicted that GUS inhibition readily occurs owing to the daily intake of common plant foods. The current study offers highly safe inhibitors for GUS, as well demonstrates a novel nonantioxidant target for VE providing new insights into the vitamin’s gastrointestinal protection.
研究领域:
生物化学
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药理学
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分子生物学
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酶学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2013
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IF 1.304
Yi Hu,Xiangjun Jiang,Suwen Wu,Ling Jiang,He Huang
Vitamin E succinate was synthesized by interfacial activated Candida rugosa lipase (CRL) encapsulated in sol-gel materials. The effects of various immobilization parameters were investigated. The optimum conditions were found to consist of n -propyltrimethoxysilane/tetraethoxysilane molar ratio = 1/1, water/silane molar ratio ( R value) = 20, lipase loading = 0.5 mg/ml sol, and PEG400 loading = 12 μl/ml sol. Compared with free enzymes, sol-gel encapsulated enzymes kept its activity of 70.58% and achieved 2.6-fold increased stability after 18 h incubation in phosphate buffer (0.025 mol/L, pH = 7.0) at 50 °C. Based on the interfacial characteristics of CRL, five kinds of surfactants were used for activation. The results demonstrate that olive oil was the most effective in activating CRL. The esterification activity of vitamin E succinate synthesis by interfacial activated CRL encapsulated in sol-gel materials reached up to 6.7-fold and 1.43-fold that of free enzyme and non-interfacial activated enzyme, respectively.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Bingchao Cheng,Hao Pan,Dandan Liu,Dongyang Li,Jinyu Li,Shihui Yu,Guoxin Tan,Weisan Pan
The purpose of this work was to develop a d -α-tocopherol polyethylene glycol 1000 succinate (TPGS) decorated nanodiamond (ND) system loading water-insoluble curcumin (ND/CUR/TPGS) to improve the colloidal dispersity and oral bioavailability of the preparation. CUR was physically loaded into ND clusters, then TPGS was coated to the ND/CUR complex forming amorphous nanostructure on the interparticle nanocage of the ND substrate. The formulation of the nanocomplexes was optimized using response surface methodology, and the optimal ND/CUR/TPGS showed small particle size (196.32 nm), high drug loading efficiency (81.59%) and core-shell structure. In vitro release study demonstrated that the nanocomplexes provided a sustained release behavior. The absorptive concentration of ND/CUR/TPGS was dramatically improved in total intestinal tract compared with CUR suspension, and the absorption was controlled by multiple transcytosis mechanisms. Furthermore, the pharmacokinetic studies demonstrated that ND/CUR/TPGS had significantly higher C max (4.50-fold), larger AUC 0–t (10.67-fold), and longer MRT 0–t (3.07-fold) in contrast with that of CUR suspension. Therefore, ND/CUR/TPGS presented great potential for oral delivery of insoluble and poorly permeable drugs.
研究领域:
药剂学
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纳米技术
·
药物递送系统
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）