JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yudie Sun,Aobo Feng,Ruilai Zhang,Kehong Quan,Zefeng Hua,Kui Zhang
Bacterial contamination is a very serious health and environmental problem, with the main source of toxicity being lipopolysaccharides in the cell walls of Gram-negative bacteria. Therefore, the development of effective analytical methods is crucial for the detection of lipopolysaccharide content. This work facilitates the efficient generation of precisely adjustable dual-mode signals for LPS detection in surface-enhanced Raman spectroscopy (SERS) and electrochemiluminescence (ECL) by inducing anisotropic morphological evolution of Au@Ag nanocubes (Au@AgNCs) through poly-cytosine (poly-C) DNA. In the presence of LPS, the released probe triggers rolling circle amplification (RCA) and generates cyclic poly-C DNA, which subsequently induces the etching of Ag composites on Au@AgNCs. This etching process leads to changes in both the morphology and composition of Au@AgNCs composite materials, resulting in a decrease in SERS signals. The poly-C DNA was subsequently adsorbed with Ag + and then reduced to silver nanoclusters (AgNC) by NaBH 4 , serving as an ECL signal source. Under optimal experimental conditions, both SERS and ECL demonstrated linear ranges spanning from 1 fg/mL to 1 ng/mL, with detection limits of 0.29 fg/mL and 0.14 fg/mL, respectively. Moreover, the SERS-ECL dual-mode sensor facilitates the accurate discrimination of Gram-negative bacteria in diverse real samples. It demonstrates promising applications in the areas of food safety, water quality monitoring, and clinical bacterial detection.
研究领域:
生物分析
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环境科学
·
医学检测
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PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2026
·
IF 5.8
Fuge Chai,Jing Wang,Siyi Sun,Yongping Dong,Mingfu Ye
Cathodic ECL of TiO 2 was greatly enhanced by carboxylated MWCNTs. • TiVC MXene QDs were loaded onto MOF to form stable nanocomposite. • TiVC/MOF nanocomposite exhibited inhibiting effect on the cathodic ECL of TiO 2 . • An ECL sensor for chloramphenicol was fabricated based on its inhibiting effect. In this study, TiO 2 nanoparticles were employed as novel luminophore to obtain cathodic electrochemiluminescence (ECL) signal in the presence of K 2 S 2 O 8 under neutral aqueous condition. Carboxylated multi-walled carbon nanotubes (f-MWCNTs) served as co-reactant accelerators to facilitate the reduction of K 2 S 2 O 8 , resulting in greatly enhanced cathodic ECL signal. TiVC MXene quantum dots (TiVC QDs) were loaded onto MOF to form stable nanocomposite (TiVC/MOF) which could effectively suppress the cathodic ECL emission by blocking interfacial electron transfer and consuming SO 4 •− via chemical reaction. Based on the inhibiting effect of TiVC/MOF nanocomposite, an “off-on” mode ECL sensor was fabricated for the sensitive detection of chloramphenicol (CAP). Under the optimal condition, the proposed sensor exhibited a linear range of 0.1 μM-6 μM with a detection limit of 3.62 nM. Moreover, the ECL sensor showed satisfactory analytical performance in real samples, offering a reliable strategy for the sensitive detection of CAP. Carboxylated multi-walled carbon nanotubes (f-MWCNTs) could act as co-reactant accelerators to greatly enhance the cathodic ECL of TiO 2 nanoparticles. TiVC MXene QDs were loaded onto MOF to form stable nanocomposite (TiVC/MOF) which could effectively suppress the cathodic ECL emission. Based on the inhibiting effect of TiVC/MOF nanocomposite, an “off-on” mode ECL sensor was fabricated for the sensitive detection of chloramphenicol (CAP). The ECL sensor showed satisfactory analytical performance in real samples, offering a reliable strategy for the sensitive detection of CAP. Download: Download high-res image (178KB) Download: Download full-size image
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高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Longmei Yao,Jiajia Zhi,Wenchang Wang,Qingyi Li,Ding Jiang,Xiaohui Chen,Zhidong Chen
As a synthetic estrogen, diethylstilbestrol (DES) residues in water pose a threat to human health. In this work, a novel electrochemiluminescence resonance energy transfer (ECL-RET) aptamer sensor based on NCDs@Ag 3 PO 4 as a resonance energy transfer donor and Cu 2+ doped Eu MOF (Cu: Eu MOF) as an efficient resonance energy transfer acceptor for the detection of diethylstilbestrol (DES) was proposed for the first time. The aptamer sensor uses Ag 3 PO 4 nanoparticles loaded with nitrogen-doped carbon quantum dots (NCDs) as the ECL emitter, which improves the electron transfer efficiency of the sensor and promotes the generation of SO 4 ·- radicals, thereby improving the luminescence intensity and stability of the ECL sensor. The energy acceptor Cu: Eu MOF binds to the apt on NCDs@Ag 3 PO 4 by complementary base pairing through complementary DNA (cDNA). When DES is present in the sample, DES competes with the energy acceptor. The stronger interaction between DES and apt leads to the shedding of Cu: Eu MOF-cDNA from the electrode and the recovery of ECL signal. Based on this, under optimal conditions, the linear range of the sensor for detecting DES is 1.0 × 10 −13 –1.0 × 10 −6 M, and the detection limit is 7.4 × 10 −14 M (S/N = 3). The developed aptasensor showed excellent recognition ability for residual DES in actual water samples. The sensor has superior measurement ability and potential application value in the field of environmental water quality monitoring.
研究领域:
分析化学
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环境科学
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生物传感技术
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Xiaojian Li,Yaoguang Wang,Li Shi,Hongmin Ma,Yong Zhang,Bin Du,Dan Wu,Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy) 3 2+ /silica/Fe 3 O 4 nanomaterials (CRuSi-Fe 3 O 4 ) were synthesized through W/O microemulsion route. The added Fe 3 O 4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe 3 O 4 . In addition, the layered structure of CRuSi-Fe 3 O 4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor. Then the quenching probe glucose functionalized NiCo 2 S 4 nanoparticles-grown on carboxylic graphene (NiCo 2 S 4 -COOH-rGO@Glu) was anchored on the modified-electrode via the specific carbohydrate-Con A interaction. Here, NiCo 2 S 4 was used to quench the ECL of CRuSi-Fe 3 O 4 , graphene was used to grow NiCo 2 S 4 nanoparticles as carrier materials and glucose was served as the recognition element for bounding Con A. Therefore, a desirable quenching ECL signal was measured with S 2 O 8 2- as the coreactant of CRuSi-Fe 3 O 4 . Under the optimization of determination conditions, a linear response range for Con A from 0.5 pg mL −1 to 100 ng mL −1 was obtained, and the detection limit was calculated to be 0.18 pg mL −1 (S/N=3).
研究领域:
生物传感
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材料科学
·
分析化学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Kai Zhang,Zhenqiang Fan,Yuedi Ding,Minhao Xie
In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19.
研究领域:
生物技术
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纳米技术
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分子生物学
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分析化学
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病毒学
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 25
·7095-7103
·
IF 3.125
Yu Linpo,Liu Yang,Zhou Ming
Ruthenium(II) complexes with carboxylic acid as a bioconjugatable group, i.e., [Ru(bathophenanthroline disulfonate)(2,2′-bipyridine)(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)] 0 , (C 49 H 38 N 6 O 8 S 2 Ru), and [Ru(bathophenanthroline disulfonate) 2 (4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)] 2− · 2Na + , (C 63 H 44 N 6 O 14 S 4 RuNa 2 ) were characterized spectroscopically and electrochemically. As potential labels for electrochemiluminescence (ECL) immunoassays, the ECL intensities of the free labels in homogenous aqueous buffer solutions were compared under a condition that is similar to the one employed by a commercial clinical immunoassay system. The two labels were found to be more emissive and, thus, can be detected at 10 - 12 pM compared with 5× 10 −12 pM of the label currently used in the commercial ECL system. Furthermore, the improved ECL emission of the free labels in homogenous solutions was proven to be translated into more intense ECL signal in heterogeneous sandwich immunoassay and, thus, leading to a lower limit of detection in immunoassay. The data obtained from these ECL labels shed light on the further development of ECL-based clinical immunoassay technology.
Electrochemiluminescence immunoassays were carried out with three different ruthenium(II) complex labels. It was proved that the higher signal intensities found with the novel labels in homogeneous solutions were maintained in heterogeneous sandwich format.
研究领域:
分析化学
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生物技术
·
临床诊断
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PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047508
·
IF 3.9
Jinhong Liu,Rui Yin,Xiaoyu Wang,Fei Li,Linru Xu
Oxytetracycline (OTC) is one of the widely used antibiotics in veterinary practices because of its broad-spectrum antibacterial activity and low cost. However, drug abuse has triggered adverse effects on human health, which brings a growing demand for on-site diagnosis of OTC residues in animal-derived foods. In this study, we demonstrated the combined use of interdigital electrode microarray and electrochemiluminescence (ECL) imaging for parallel multiplex measurement of OTC. Well-ordered and vertically aligned mesoporous silica nanochannels modified on microarray substrate could exert a strong electrostatic attraction to the ECL luminophores and accelerate their mass transport to generate enhanced ECL signal. The performance of the integrated ECL microarray sensor was fully validated with respect to linearity (0.5 μM to 50 μM), sensitivity (limit of detection 0.26 μM), accuracy (recovery rate between 96.78% and 106.1%), low operating sample volume (480 nL), short assay time (1.5 min), and antifouling ability toward complex media. The multiplex microarray platform can serve as a simple, fast, and low-cost tool for the detection of a wide spectrum of antibiotics in the field of food safety.
研究领域:
分析化学
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纳米技术
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食品科学
·
生物技术
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Xiaohong Liu,Libo Li,Fang Li,Wanlin Zhao,Lijun Luo,Xiaoya Bi,Xia Li,Tianyan You
Signal amplification provides an effective way to improve detection performance. Herein, an ultrasensitive electrochemiluminescence (ECL) aptasensor for Pb 2+ detection was developed based on a dual signal-amplification strategy of the abscission of a quencher and the generation of a G-quadruplex by one-step and simultaneous way. Nitrogen-doped carbon quantum dots linked with complementary DNA (cDNA-NCQDs) at the sensing interface was applied as the quencher of a tris(4,4′-dicarboxylic acid-2,2′-bipyridyl)ruthenium(II) (Ru(dcbpy) 3 2+ )/tripropylamine system to minimize the ECL signal due to the intermolecular hydrogen bond-induced energy-transfer process. Upon the addition of Pb 2+ , its specific binding with the aptamer triggered the abscission of cDNA-NCQDs, accompanied by the formation of G-quadruplex on the surface of the electrode, both of which amplified the intensity of the light emission. The ECL amplification efficiency induced by the above two mechanisms (78.6%) was valuably greater than that of their sum value (69.3%). This synergistic effect resulted in high detection sensitivity of the ECL aptasensor, which allowed to thereby obtain Pb 2+ measurements in the range of 1 fM - 10 nM with an ultra-low detection limit of 0.19 fM. The Pb 2+ -mediated synergistic signal-amplification ECL strategy can provide a new approach for integrating various amplification strategies.
研究领域:
分析化学
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环境科学
·
材料科学
·
纳米技术
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PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Libo Li,Bainian Chen,Xiaohong Liu,Panao Jiang,Lijun Luo,Xia Li,Tianyan You
Divalent mercury ion (Hg 2+ ) is widely distributed in the ecological environment and harmful to the human body and the environment. Herein, a highly sensitive and selective ‘on–off-on’ electrochemiluminescence (ECL) aptamer sensing method based on the dual signal amplification strategy of a self-enhanced luminophore and ECL resonance energy transfer (ECL-RET) was developed to detect Hg 2+ . In detail, the first ‘switch-on’ state was obtained by the self-enhanced luminophore of Ru(bpy) 3 2+ -doped silica nanoparticle-nitrogen-doped graphene quantum dots ( [email protected] 2 -NGQDs) modified glassy carbon electrode, which significantly amplified the ECL intensity compared with the mixture of [email protected] 2 and NGQDs . Later, the ‘switch-off’ state was induced by gold nanoparticles (AuNPs), which acted the acceptor in the ECL-RET system between AuNPs and [email protected] 2 -NGQDs due to their good spectral overlap. This combination of the self-enhanced luminophore and ECL-RET provided a novel dual signal amplification strategy, which led to a high signal-to-noise ratio and high analysis sensitivity. Finally, upon the addition of Hg 2+ , the formation of a stable T-Hg 2+ -T structure induced the release of AuNPs from the sensing interface and halted the ECL-RET process, resulting in ECL signal recovery (the second ‘switch-on’ state). For the detection of Hg 2+ , the proposed ECL sensor exhibited a wide linear range from 1 × 10 −14 to 1 × 10 −6 M and a highly sensitive detection limit of 3.0 fM. Finally, the feasibility of the developed method in water sample analysis was investigated.
研究领域:
分析化学
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环境科学
·
材料科学
·
纳米技术
·
传感器技术
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Kai Zhang,Zhenqiang Fan,Bo Yao,Tingting Zhang,Yuedi Ding,Sha Zhu,Minhao Xie
2019 novel coronavirus (2019-nCoV) with strong contagion in the crowd, has ravaged worldwide and severely impacts the human health and epidemic prevention system, by producing a series of significant stress reactions in the body to induce further cytokine storm. Transcription factors (TFs) served as essential DNA binding proteins play an integral role in regulating cytokine storm, and the detection of it in the human coronavirus environment provides especially valuable approaches to diagnosis and treatment of 2019-nCoV and development of antiviral drugs. In this work, an entropy-driven electrochemiluminescence (ECL) biosensor was constructed for ultra-sensitive bioassay of NF-κB p50. The strategy primarily capitalizing the splendid double-stranded DNA (dsDNA) binding properties of transcription factors, employing GOAu-Ru composite material as ECL emitter, utilizing entropy-driven reactions for signal amplification method, offered a repeatable proposal for TFs detection. In the absence of TFs, the released DNA1 further went in the entropy-driven reaction, contributing to an “ECL off” state. However, in the presence of TFs, the dsDNA avoided being digested, which blocked DNA1 for participating in the entropy-driven reaction, and the system exhibited an “ECL on” state. Most importantly, the ECL bioanalytical method denoted broad application prospects for NF-κB p50 detection with a lower detection limit (9.1 pM).
研究领域:
分析化学
·
生物化学
·
分子生物学
·
病毒学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Hongguo Zhai,Yue Wang,Lingjun Geng,Qi Guo,Yuhao Zhang,Qingqing Yang,Xia Sun,Yemin Guo,Yanyan Zhang
In this paper, an electrochemiluminescence (ECL) biosensor was constructed using Bi 2 S 3 @Au nanoflowers as the based nanomaterial and [email protected] and CdS QDs as independent ECL emission signal respectively. As the substrate of the working electrode, Bi 2 S 3 @Au nanoflowers improved the effective area of electrode and accelerated electron transfer rate between gold nanoparticles and aptamer, provided a good interface environment for the loading of luminescent materials. Then, the [email protected] functionalized DNA2 probe was used as an independent ECL signal source under positive potential and recognized Cd(II), while the CdS QDs functionalized DNA3 probe was used as an independent ECL signal source under negative potential and recognized ampicillin. The simultaneous detection of Cd(II) and ampicillin in different concentrations are realized. This sensor not only has good selectivity and high sensitivity in real sample detection, but also open up a novel way to construct multi-target ECL biosensor for simultaneous detection.
研究领域:
分析化学
·
环境科学
·
食品安全
·
纳米材料
·
生物化学
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DOI
PubMed
高影响因子
ANALYST
·
2024
·Vol. 149
·期 6
·1746-1752
·
IF 3.6
Xiaohui Chen,Jing Wen,Xueling Shan,Wenchang Wang,Zhidong Chen
In this work, the supersensitive and selective determination of lincomycin (Lin) was achieved using a novel electroluminescent (ECL) aptasensor based on the synergistic integration of gold functionalized upconversion nanoparticles (UCNPs) and thiolated 3,4,9,10-perylene tetracarboxylic acid (PTCA). The integration of two luminophores of UCNPs and PTCA combined the merits of the cathodoluminescence stability of UCNPs and the high quantum yield of PTCA, which significantly promoted the ECL signal and analytical performance of the proposed sensor. The introduction of gold nanoparticles in UCNPs can not only improve the conductivity and ECL performance of UCNPs but also cause them to easily integrate with thiolated PTCA (t-PTCA) via an Au-S bond. The ECL signal of UCNPs@Au/t-PTCA/GCE was almost twice as strong as that of t-PTCA/GCE and tenfold higher than that of UCNPs@Au/GCE. Because of the non-conductive protein of the Lin aptamer, the ECL intensity of apt/UCNPs@Au/t-PTCA/GCE noticeably decreased. In the presence of Lin, the aptamer was pulled down from the sensing interface, resulting in the recovery of the ECL intensity of the sensor. Under optimal conditions, our proposed sensor can quantify the concentration of Lin in the range from 1.0 × 10−15 to 1.0 × 10−7 M with a low detection limit of 2.4 × 10−16 M (S/N = 3), exhibiting high sensitivity and specificity for the determination of Lin.
研究领域:
分析化学
·
生物传感
·
药物检测
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）