ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 47
·67203-67213
·
IF 4.223
Xu Xia,Zhang Ling,Xue Yingang,Gao Yu,Wang Liping,Peng Mingguo,Jiang Shanqing,Zhang Qiuya
Much more attention has been poured into microplastic pollution in freshwater systems recently. In the present study, the pollution of microplastics (MPs) in surface water and freshwater fish (crucian carp, etc.) were investigated from Gehu Lake, which is the second largest lake in southern Jiangsu after Taihu Lake. The result manifested that the average abundance of MPs was respectively 6.33±2.67 n/L for surface water and 10.7 items per individual for freshwater fish. The distribution of MPs in Gehu Lake varied from place to place, with the highest abundance of MPs was observed in the two estuaries of the eastern part of the lake. It was speculated that topographical factors and human factors were the main factors affecting the abundance and distribution of MPs. Transparent fibers were the main type of MPs in water samples, accounting for 69.70% of all detected particles. Meanwhile, most of the MPs ingested by freshwater fish were fibers, and the main colors were transparent and blue. In addition, the dominant size of the MPs was between 0.1 to 0.5 mm in water and fish samples. Moreover, PES, man-made fiber, and PP were the dominant polymer types in the surface water and fish samples. The results of this investigation can provide basic data for the research and management of MPs in freshwater systems.
研究领域:
环境科学
·
生态学
✓ 已验证
DOI
高影响因子
Collagen and Leather
·
2024
·Vol. 6
·期 1
·1-11
·
IF 0
Dai Hongjie,Cao Yingnan,Fu Yu,Tang Mi,Feng Xin,Ma Liang,Zhang Yuhao
Fish scales, considered as low-value by-products, contain peptides and hydroxyapatite that can be applied to produce peptide chelated calcium directly. This study developed a sustainable and one-pot fabrication method for the peptide-chelated calcium from fish scale hydrolysates (FSP-Ca). During pepsin hydrolysis, the releases of peptides (FSP), calcium, and phosphate from fish scales occurred simultaneously, and the chelation was also effectively performed. After a 6-h hydrolysis, the yield of FSP was 46.18%, and the dissolution rate of calcium was 49.53%. Under the optimal conditions (pH 7, chelation time of 25 min, and chelation temperature of 48 °C), a high chelation rate of 86.16% was obtained, with a calcium content of 81.8 mg/g. The results of UV absorption, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) confirmed the successful chelation between FSP and calcium derived from fish scales. The –NH 2 , –COO – , N–H, C=O, C–H, and –OH groups in FSP participated in the formation of FSP-Ca. Graphical Abstract
研究领域:
食品科学
·
生物材料
·
环境科学
✓ 已验证
DOI
ADVANCED MATERIALS
·
2026
·
IF 29.1
Ziyu Shao,Zhihui Dong,Zijian Xu,Zeye Wang,Mingrui Wu,Nifang Zhao,Meng Li,Weiwei Gao,Hao Bai
Fatigue failure remains a critical challenge for soft materials used in biomedical implants and soft robotics. Existing strategies, including stiff reinforcement and microstructural alignment, can suppress crack growth but often introduce anisotropy and poor resistance to multidirectional loading. In addition, a persistent orders-of-magnitude disparity remains between fracture toughness and fatigue threshold of synthetic soft materials. Here, the fish bladder of silver carp, characterized by a multilayered hierarchical fibrous architecture, is shown to exhibit extraordinary isotropic fatigue resistance (∼6000 J·m − 2 ) approaching its fracture toughness. This behavior originates from a “compliance for resistance” mechanism that couples dynamic fiber straightening and rotation, sacrificial bond breakage and reassociation, and layer-specific fiber reorientation with the resulting interfacial sliding to dissipate energy under cyclic loading. Guided by these principles, a biohybrid hydrogel is designed to reproduce this multiscale anti-fatigue mechanism, achieving a high fatigue threshold (>3000 J·m − 2 ) together with robust antifouling performance. These results uncover fundamental design principles of fatigue-resistant biological soft tissues and provide a general strategy for engineering durable soft materials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Reports Methods
·
2026
·
IF 5.8
Zhaohui Cao,Wenlong Zuo,Lanxiang Wang,Lei Dai
Spatial mapping of microbial communities at single-cell resolution is opening up dimensions to understand microbiome organization and function. However, current fluorescence in situ hybridization (FISH) methods for microbiomes are limited by multiplexity and scalability. Here, we present the sequential error-robust FISH spatial mapping platform (SEER-Map) for fully automated imaging of complex microbial communities. We show that an integrated platform of fluidics control and fluorescence microscopy can perform 40 rounds of sequential FISH. We apply SEER-Map to profile complex microbial communities colonized on plant roots and identify distinct spatial patterns and species co-occurrence at the micron-scale. Our work establishes SEER-Map as a high-throughput and scalable platform for high-resolution spatial profiling of microbiomes.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Bo Zhang,Kang Chen,Jiahao Niu,Qi Lu,Wei Yu,Yanan Xiao,Chengkun Dong,Yi Ni,Chengxin Zhao
The 3 mol% Mo 6+ − doped In 2 O 3 nanowires exhibit a high response of 233.5 and a LOD as low as 25.4 ppb for TMA detection. • Substitutional Mo 6+ and surface MoO 3 jointly regulate surface oxygen chemistry and charge transfer. • Mo 6+ doping induces concurrent regulation of lattice, electronic, and surface properties in In 2 O 3 nanowires. • The Mo 6+ − doped In 2 O 3 sensor demonstrates practical application in fish-freshness monitoring through TMA detection. Marine fish are highly susceptible to deterioration during capture, transportation, and storage, accompanied by the generation of malodorous trimethylamine (TMA), making rapid TMA detection highly desirable for freshness assessment. In this work, Mo − containing In 2 O 3 nanowires with different nominal Mo/In ratios were synthesized, and their TMA sensing properties were systematically investigated. The 3 mol% Mo sample (3IMO) exhibited the optimal sensing performance, with the optimal operating temperature decreasing from 220 °C for undoped 0IMO to 200 °C, while the response toward 100 ppm TMA increased markedly from 32.2 to 233.5. The 3IMO sensor also exhibited a rapid response time of 2 s, a recovery time of 262 s, and a low limit of detection (LOD, 0.0254 ppm). Structural analyses reveal that the introduced Mo exists predominantly as substitutional Mo 6+ incorporated into the In 2 O 3 lattice, accompanied by a minor amount of highly dispersed surface MoO 3 nanocrystallites. The enhanced sensing behavior is therefore attributed to the synergistic contributions of these two Mo configurations: lattice-incorporated Mo 6+ provides the dominant regulation of carrier/surface-charge characteristics and surface oxygen chemistry, whereas trace surface MoO 3 provides auxiliary interfacial charge modulation and possible Lewis-acidic adsorption sites. Combined with the pronounced enrichment of chemisorbed oxygen species, these effects facilitate TMA surface redox reactions and amplify resistance modulation. Furthermore, practical fish-storage experiments demonstrate that the 3IMO sensor can effectively track the freshness deterioration, showing consistency with pH and Quality Index Method (QIM) sensory evaluations. These results demonstrate the potential of Mo − modified In 2 O 3 nanowires for sensitive TMA detection and fish freshness monitoring. Download: Download high-res image (236KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 12
·2075
·
IF 5.1
Zongyun Du,Jihui Lao,Yuyan Jiang,Jingyu Liu,Shili Liu,Jianbo Zheng,Fei Li,Yongyi Jia,Zhimin Gu,Jun Chen,Xiao Huang
Cell-cultured meat production relies on stable, proliferative seed cells, commonly sourced from muscle satellite cells (MuSCs) and adipose-derived mesenchymal stem cells (AD-MSCs). However, establishing such cell lines in fish species remains technically challenging. While pluripotent stem cells (e.g., ESCs/MSCs) offer alternatives, their differentiation efficiency and predictability are limited. Here, we developed TCCF2022, a novel caudal fin-derived cell line from Topmouth culter (Culter alburnus), which expresses pluripotency markers (AP,Oct4,Sox2,Klf4, andNanog) and aggregated growth to form 3D spheroids. Forskolin supplementation enhanced pluripotency maintenance. The presence of adipogenic and myogenic lineage cells within the 3D spheroids was confirmed, demonstrating their potential as seed cells for cell-cultured meat. Using a small-molecule cocktail 5LRCF (5-Azacytidine, LY411575, RepSox, CHIR99021, and Forskolin), we successfully differentiated TCCF2022 cells into functional myotubes. Additionally, we established a method to induce the differentiation of TCCF2022 cells into adipocytes simultaneously. Thus, the TCCF2022 cell line can be used to improve muscle fiber formation and lipid composition, potentially enhancing the nutritional profile and flavor of cultured fish meat.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensor Review
·
2025
·
IF 1.4
Zhaopeng Li,Pei Li,Peisi Yin,Xin Zhao,Yangting Hu,Fei Sun,Xia Sun,Zhenhe Wang
Purpose This paper aims to develop a highly sensitive resistive gas sensor for accurately detecting ethyl formate to achieve reliable and real-time monitoring of fish meal spoilage. Design/methodology/approach Based on the one-step solvothermal reduction method, in a specific triethylene glycol solution environment and high temperature, the 3D ZnO sensing material with a jackfruit-like structure was prepared for detecting ethyl formate gas in fish meal. Findings The gas sensor based on 3D ZnO displays high sensor response (69.68–100 ppm) at 280°C and 43% RH and good sensor response (12.18–100 ppm) at 280°C and 80% RH, ultra-low detection limit of 10 ppb at 43% RH, excellent selectivity, repeatability and long-term stability. The sensing mechanism is due to the gain or loss of electrons caused by the sensor surface reaction. The unique jackfruit-like structure, abundant oxygen vacancies and large surface area may be another factor contributing to its excellent ethyl formate gas sensing performance. Originality/value The authors first developed an ethyl formate gas sensor, and the results were compared with the previously published data. The analysis showed the ZnO gas sensor demonstrated excellent sensing performance. This work highlights the potential of high response ethyl formate gas sensors to accurately evaluate fish meal quality.
研究领域:
材料科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Wanjun Xu,Yulong Bao,Yue Zhou,Hui Hong,Ruichang Gao
This study aimed to investigate the effect of oxidation on fish gelatin and its emulsifying properties. Fish gelatin was oxidized with varying concentrations of H 2 O 2 (0–30 mM). Increased concentrations of the oxidant led to a decrease in amino acids in the gelatin, including glycine, lysine, and arginine. Additionally, the relative content of ordered secondary structure and triple helix fractions decreased. Zeta potential decreased, while particle size, surface hydrophobicity, and water contact angle increased. Regarding emulsifying behavior, oxidation promoted the adsorption of gelatin to the oil–water interface and reduced interfacial tension. With increased degrees of oxidation, the zeta potential and size of the emulsion droplets decreased. The oxidized gelatin exhibited better emulsifying activity but worse emulsifying stability. Based on these results, a mechanism for how oxidation affects the emulsifying properties of gelatin was proposed: the increase in gelatin’s hydrophobicity and the decrease in triple helix structure induced by oxidation reduced the interfacial tension at the oil–water interface. This promoted protein adsorption at the oil–water interface, allowing the formation of smaller oil droplets and enhancing gelatin’s emulsifying activity. However, the decrease in electrostatic repulsion between emulsion droplets and the decrease in solution viscosity increased the flocculation and aggregation of oil droplets, ultimately weakening the emulsifying stability of gelatin.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Polymers
·
2024
·Vol. 16
·期 11
·1538
·
IF 4.7
Boxuan Yao,Long Gu,Li Huang,Ruichun Li,Ze Fan,Zhongxiang Chen,Dongli Qin,Lei Gao
In this study, a highly efficient magnetic molecularly imprinted polymer nanocomposite material was prepared using multi-walled carbon nanotubes as carriers. The characterization of the obtained nanocomposite material was conducted using Fourier transform infrared spectroscopy, a vibrating sample magnetometer, a thermogravimetric analyzer, a scanning electron microscope, and a transmission electron microscope. The adsorption properties of the nanocomposite material were evaluated through adsorption experiments, including static adsorption, dynamic adsorption, and selective recognition studies. The prepared nanocomposite material, serving as a selective adsorbent, was applied in magnetic solid-phase extraction. Subsequently, the derivatized samples were analyzed for glucose in fish serum using liquid chromatography–tandem mass spectrometry. Under optimal conditions, the detection limit was 0.30 ng/mL, the quantitation limit was 0.99 ng/mL, satisfactory spiked recovery rates were obtained, and the relative standard deviation was less than 1.1%. Using 2-deoxy-D-ribose as the template molecule and a structural analog of glucose allowed us to eliminate the potential template leakage in qualitative and quantitative analyses, effectively avoiding the issues of false positives and potential quantitative errors, compared to traditional methods. A method for detecting glucose levels in fish serum based on molecularly imprinted polymer technology has been successfully developed to determine the stress and health levels of fish.
研究领域:
分析化学
·
材料科学
·
生物化学
·
环境科学
·
水产养殖
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Rongqiang Chen,Shuai Zhang,Jiao Jiao Sun,Lijuan Xu,Yuan Duan,Fengchen Li,Yanhui Han,Xiao Fu,Meng Wang,Yong Hong Meng,Yurong Guo
Compared to animal gelatin, the application of fish gelatin (FG) in the food industry is limited due to its lower gel hardness, gelation temperature, and melting temperature. To address these issues, we developed hybrid gels using carboxymethyl chitin nanofibers (CMC-NFs) with FG to enhance its gel properties. The chitin was carboxymethylated by monochloroacetic acid, increasing the negative potential from −6 mV to −35 mV. This modified chitin was then defibrillated into CMC-NFs through high-pressure homogenization to improve its water dispersibility. The addition of 0.1% CMC-NFs significantly increased the hardness of FG gels from 533 g to 989 g, which was notably higher than the ∼600 g hardness of porcine gelatin. Additionally, the gelation and melting temperatures of FG reached 17 °C and 32 °C, respectively, which are very close to the 20 °C and 35 °C of porcine gelatin. Mechanistic studies revealed that FG and CMC-NFs (0.1%) molecules formed a synergistic gel network, facilitated by hydrogen bonding and hydrophobic interactions. This interaction significantly increased the α-helix content in the secondary structure of the FG protein from 15.26% to 21.18%, thereby enhancing the FG-dominated three-dimensional network structure with CMC-NF molecules. Increasing the concentration of CMC-NFs further elevated the gel's melting temperature while reducing its hardness due to phase separation. This study not only presents a viable method for enhancing the gelling properties of modified FG but also offers a feasible solution for expanding FG's applications in the food industry.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2025
·Vol. 2025
·期 1
·6298724
·
IF 2.5
Niuniu Bai,Jianlin He,Bihong Hong,Zelong Wu,Liping Wang,Zongze Shao
Background: Traditional fish sauce is made from fish that have been coated in 30%–40% sea salt and fermented for up to 2 years. The preparation requires a long time, and the fish source contains considerable biogenic amines. In this report, by measuring the growth curve and identifying the intracellular enzyme and the activity of protease, B. zhangzhouensis with the highest activity of protease production was selected from eight strains, which were applied to the fermentation process of fish sauce. Results: Optimized by response surface methodology, the content of amino nitrogen was taken as the response value, and the best fermentation conditions were 4.15% inocula, pH 9.13, and 36.3°C. Under the optimized conditions, fish sauce samples were obtained by fermentation with B. zhangzhouensis for 15 h, 3 days, 7 days, 15 days, and 30 days, respectively. The contents of amino nitrogen, total nitrogen, biogenic amine, and various flavor substances were determined and compared with the traditional 2-year-fermented fish sauce. As a result, the 7-day-fermented fish sauce using B. zhangzhouensis had a similar composition of flavor substances to traditional fish sauce, but a much lower level of biogenic amines. Conclusion: The new method of preparing fish sauce with a fermentation condition of 4.15% B. zhangzhouensis , pH 9.13, and 36.3°C can improve the production efficiency of fish sauce, and the flavor is similar to that of traditional fish sauce.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yingfei Su,Shengliang Zhang,Yingqi Jiang,Enbo Xu,Donghong Liu,Qihe Chen
Cultured meat has emerged as a promising approach to enhancing global food sustainability. Current production methods, however, rely mainly on single-cell-type culture, which fails to replicate the complex composition of conventional meat. In this study, a scaffold-free co-culture system was established to generate structured microtissue comprising highly differentiated muscle and fat cells. Co-cultured spheroids with the ratios of piscine adipose-derived stem cells (PADSCs) to piscine satellite cells (PSCs) being 5:5, 7:3 and 9:1 were investigated, and the result showed that all ratios remained robust and highly viable throughout the differentiation process and successfully induced the formation of bionic tissue containing both myotubes and adipocytes. Based on transcriptome data, the cluster analysis indicated that co-cultured spheroids (the ratio of PADSCs to PSCs was 7:3) were similar to the PADSC spheroids, and the expression levels of cell cycle-related and extracellular matrix-related genes were biased towards those of PADSC spheroids. These results indicated that these co-cultured spheroids held promise as building blocks for further tissue assembly and offered a promising approach for scaling up the production of structured cultured fish meat.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）