Analytical Methods
·
2020
·Vol. 12
·期 7
·970-976
·
IF 2.596
Wenjing Wang,Wenyi Lv,Ziyu Pan,Lei Zhan,Chengzhi Huang
The development of an accurate and simple detection system for antibiotics is of paramount importance for food safety and human health due to their abuse in clinical practice and animal husbandry. In this work, we have constructed novel enhanced nanoflares by incorporating another organic quencher BHQ-2 into the conventional spherical nucleic acid nanoconstruct. The fluorescence of the donor TAMRA was quenched simultaneously by AuNPs and BHQ-2 due to fluorescence resonance energy transfer (FRET). Compared to traditional nanoflares, the one-donor-two-acceptor strategy enhanced the energy transfer efficiency and increased the detection sensitivity for ampicillin (AMP) owing to a reduced background signal. In the presence of AMP, the specific binding of aptamers on the enhanced nanoflares to the target triggered fluorescence recovery in a concentration-dependent manner. With this approach, a detection limit of 0.65 ng mL−1 and a linear dynamic range of 1.8–20 ng mL−1 were achieved for AMP. Moreover, this method also displayed high specificity, good reproducibility, and acceptable accuracy for detection of AMP in spiked milk samples and pharmaceutical products. The principle of the present nanoflares can be extended to other antibiotics for which aptamers are available, providing new insights into the fabrication of nanoscale energy transfer-based aptasensors for antibiotic analysis.
研究领域:
生物传感
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分析化学
·
食品科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yanan Li,Jian Cao,Chuanxiao Wang,Yiyi Qiao,Jiayu Gao,Xiao Zhang,Le Wang
In this strategy, the fluorescence sensor Nap-Co-T1 employing the fluorescence resonance energy transfer (FRET) mechanism was designed and synthesized to have an efficient response to Heparin, and the FRET mechanism was explored for different excitation-emission wavelengths with different distances between the energy acceptor and the energy donor (comparing with fluorescence sensor Nap-TPA-T2). Upon the addition of Heparin, the fluorescence emission of Nap-Co-T1 was turned on at 565 nm, and the fluorescence color changed of the solution from colorless to bright yellow. The limit of detection (LOD) was as low as 0.04 μg/mL. With the addition of antagonistic protamine (PRTM) to the sensor complex with Heparin, the fluorescence emission was turned off to a certain extent, and the reversibility of the “off-on-off” system was maintained for five cycles or more. In addition, Nap-Co-T1 provides rapid and sensitive detection of Heparin in human serum albumin solution and artificial urine and is highly sensitive to environmental viscosity.
研究领域:
生物化学
·
医学检测
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DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 16
·2923
·
IF 3.06
Piaopiao Chen,Iqra Ilyas,Su He,Yichen Xing,Zhigang Jin,Chaobiao Huang
Polymer dots (Pdots) represent newly developed semiconductor polymer nanoparticles and exhibit excellent characteristics as fluorescent probes. To improve the sensitivity and biocompatibility of Pdots ratiometric pH biosensors, we synthesized 3 types of water-soluble Pdots: Pdots-PF, Pdots-PP, and Pdots-PPF by different combinations of fluorescent dyes poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadazole)] (PFBT), and fluorescein isothiocyanate (FITC). We found that Pdots-PPF exhibits optimal performance on pH sensing. PFO and FITC in Pdots-PPF produce pH-insensitive (λ = 439 nm) and pH-sensitive (λ = 517 nm) fluorescence respectively upon a single excitation at 380 nm wavelength, which enables Pdots-PPF ratiometric pH sensing ability. Förster resonance energy transfer (FRET) together with the use of PFBT amplify the FITC signal, which enables Pdots-PPF robust sensitivity to pH. The emission intensity ratio (I517/I439) of Pdots-PPF changes linearly as a function of pH within the range of pH 3.0 to 8.0. Pdots-PPF also possesses desirable reversibility and stability in pH measurement. More importantly, Pdots-PPF was successfully used for cell imaging in Hela cells, exhibiting effective cellular uptake and low cytotoxicity. Our study suggests the promising potential of Pdots-PPF as an in vivo biomarker.Keywords:Pdots;semiconductor polymer;FRET;pH sensing;cell imaging
研究领域:
生物化学
·
材料科学
·
细胞生物学
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DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Chao Xia,Yan Xu,Meng-Meng Cao,Yun-Peng Liu,Jin-Feng Xia,Dan-Yu Jiang,Guo-Hong Zhou,Rong-Jun Xie,Da-Feng Zhang,Hui-Li Li
In the present study, a kind of Eu(III) post-functionalized Zr(IV)-based metal-organic framework (UiO-66(COOH) 2 , Zr-MOF: Eu 3+ ) was synthesized and utilized as an independently luminescent probe for sensing bilirubin (BR) in human serum, a biomarker of jaundice hepatitis. It can be served as a turn-off fluorescent switch for BR because its red emission from Eu 3+ can be easily quenched by BR through a fluorescent resonant energy transfer (FRET) process between BR and its ligands, and as a result, BR is recognized successfully. Particularly, Zr-MOF: Eu 3+ has shown many appealing properties, such as high sensitivity, quick response (less than 1 min), broad response window (0–15 μM), and excellent selectivity. Most importantly, a kind of portable test paper based on Zr-MOF: Eu 3+ probe has been developed for directly assessing the level of BR in real human serum and further diagnosing bilirubin-related diseases via visually observing the luminescent color variation.
研究领域:
生物化学
·
医学诊断
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 12
·1617-1623
·
IF 2.596
Guoqiang Liu,Hongwei Ge,Ranhao Yin,Long Yu,Congming Sun,Weiru Dong,Zhenli Sun,Khalid A. Alamry,Hadi M. Marwani,Suhua Wang
To discriminate hydrogen sulfide (H2S) from thiol nucleophiles with similar reactivity is always a major challenge in developing new detection methods. In this paper, we report a colorimetric and ratiometric fluorescence probe selectively reactive to H2S, which was synthesized by hybridizing the fluorescent nitro-benz-2-oxa-1,3-diazole (NBD)-based derivative P-NBD with carbon dots (CDs). The selective reaction can be monitored by distinct ratiometric fluorescence or colorimetric variation. The nanohybrid probe exhibited two fluorescence bands centered at 440 nm and 543 nm, respectively. The yellow fluorescence at 543 nm from P-NBD is quenched by H2S, while the blue fluorescence is enhanced by weakening of the Förster resonance energy transfer (FRET) mechanism. It was observed that the apparent fluorescence color evolved from yellow to blue when exposed to different amounts of H2S, accompanied by the color change of the probe solution from yellow to red under natural light. The detection limit was estimated to be 57 nM in aqueous solution. We successfully applied this probe for the detection of H2S in real water samples, suggesting its potential application in environmental monitoring and protection.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Haimeng Pei,Yiqun Zheng,Rongmei Kong,Lian Xia,Fengli Qu
We describe a highly sensitive nanoparticle–based fluorescence resonance energy transfer (FRET) probe developed without using molecular fluorophores as donors and acceptors. The success of this work relies on the strategy that DNA scission was designed to occur to the probe when target presented, which enabled the fluorescence signal “turn-on” of graphene quantum dots (GQDs) and thus quantitative analysis . In particular, amino-modified SiO 2 NPs were initially coated by GQDs to form highly emitting SiO 2 /GQDs, followed by conjunction with DNA functionalized gold nanoparticles (Au NPs–DNA) to form SiO 2 /GQDs–DNA–Au NPs composite. Owing to the FRET interactions between the GQDs and Au NPs, the fluorescence of GQDs was effectively quenched by Au NPs. When bleomycin (BLM), a model analyte , was mixed with the probe, the fluorescence signal of GQDs would be restored due to the removal of Au NPs from the SiO 2 /GQDs surface by DNA scission treatment with BLM in the presence of Fe (II). The current FRET probe shows a good linear relationship between the fluorescence intensity and the concentration of BLM in the range from 0.5 nM to 1 μM with a detection limit of 0.2 nM. The probe also shows satisfactory results for the analysis of clinical serum samples. This method provides versatility to the application of GQDs in FRET biosensing and could be potentially extended to other similar systems by replacing the linker between the GQDs and Au NPs.
研究领域:
生物化学
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纳米医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Qin Ouyang,Yongcun Yang,Shujat Ali,Li Wang,Huanhuan Li,Quansheng Chen
Staphylococcus aureus ( S. aureus ) is a pathogenic bacterium that seriously endangers food safety. Herein, a rapid, sensitive and specific aptasensor based on upconversion fluorescence resonance energy transfer (FRET) was developed for S. aureus detection in food. Aptamer-functionalized gold nanoparticles (AuNPs-aptamers) were bonded to cDNA-modified upconversion nanoparticles (UCNPs-cDNA) by complementary pairing, resulting in fluorescence quenching. After adding S. aureus into the system, the aptamers preferentially combined with S. aureus , dissociated UCNPs-cDNA from AuNPs-aptamers, and the fluorescence was recovered. Under optimized conditions, there was a significant linear correlation between fluorescence intensity and S. aureus concentration over the range 47–4.7 × 10 7 CFU/mL (R 2 = 0.9904) with a detection limit of 10.7 CFU/mL. Furthermore, the precision and accuracy of the developed biosensor were validated using standard plate count method, yielding no significant differences. The proposed method has potential application for rapid and sensitive quantification of S. aureus in food.
研究领域:
分析化学
·
食品科学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Chang Zhang,Xin Zhang,Wei Wang,Hao Xue,Qian He,Fenglin Li,Xiaoli Wang,Qinghui Jin,Kun Zhou,Jiawen Jian
This paper presents an ammonia (NH3) fluorescent sensor based on the MAPbBr3 perovskite and the multifunctional organic material (R/S)-OBN-tCz. The incorporation of (R/S)-OBN-tCz enhances the sensor's photoluminescence (PL) intensity through Förster resonance energy transfer (FRET) and passivation effects, which consequently improves the sensitivity of the gas sensor. The MAPbBr3&(R/S)-OBN-tCz@PMMA nanofibers fabricated via electrospinning technology not only increase the specific surface area effectively, which is crucial for sensitivity improvement, but also strengthen the stability of the perovskite material under an air atmosphere. The experimental results demonstrate that the sensor maintains stable PL performance for up to 30 days in air and over 10 hours in water, while exhibiting rapid and reversible fluorescence response to NH3.
研究领域:
材料科学
·
化学传感
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产品关联
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 4
·3646-3652
·
IF 1.338
Yang Ailing,Wang Ning,Wang Yujin,Li Shunpin,Wang Lele,Yang Yun,Bao Xichang,Yang Renqiang
Silver nanoparticles (NPs) with diameters less than 25 nm, and 5, 10, 15, 20-tetrakis(4-carboxy-phenyl) porphyrin potassium salt (K4TCPPS) was synthesized. By adding silver colloids into the K4TCPPS solutions, the fluorescence concentration quenching of the K4TCPPS
solutions was improved between 1×10−6–5×10−4 M, depending on the size of the Ag NPs, the concentration of the K4TCPPS and the excitation wavelength. Under excitation wavelengths of 360 and 414 nm, the enhancement was in the range
of 0.34–22.8, and 0.71–82.46, respectively. The enhancement increased with decreasing size of the Ag NPs. At the two highest enhancements, the concentrations of the K4TCPPS solutions were 1×10−4 and 1×10−5 M respectively,
and the size of the Ag NPs was about 5 nm. The main reasons for the fluorescence enhancing effect are due to fluorescence resonance energy transfer (FRET) between K4TCPPS molecules and Ag NPs, and decreased collision quenching between free K4TCPPS molecules, and free K4TCPPS
molecules with Ag NPs. Our results showed that Ag NPs with sizes of about 5 nm can effectively improve the fluorescence concentration quenching of K4TCPPS solutions. This result also suggests that it is possible to use noble metal NPs to improve fluorescence concentration quenching.
研究领域:
材料科学
·
光学
·
生物化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Jian Xu,Hui Yu,Yue Hu,Mingzhong Chen,Shijun Shao
A novel gold nanoparticle (AuNP)-based sensor for detecting thiols in aqueous solution has been developed. Due to the weak N···Au interactions, meso -(4-pyridinyl)-substituted BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes were coordinated to AuNP surfaces, which effectively quenched the fluorescence of organic/inorganic hybrid systems. The fluorescent quenching mechanism was mainly ascribed to the highly efficient fluorescent resonance energy transfer (FRET) and the inner filter effect. In the presence of thiols, meso -(4-pyridinyl)-substituted BODIPY chromophore were displaced and released from the AuNP surfaces and thus restored the fluorescence of BODIPY chromophore. The modulation of the fluorescence quenching efficiency of BODIPY–AuNPs in the presence of thiols can achieve a large turn-on fluorescence enhancement (40-fold) in aqueous solution. The new AuNP-based fluorescence sensor displayed desired properties such as high specificity, relatively low detection limit (30 nM for Cys), appreciable water solubility and rapid response time (within 2 min for Cys/Hcy). Moreover, the sensor has been successfully applied for monitoring and imaging of intracellular thiols within living HeLa cells .
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2014
·
IF 3.511
Xiaojuan Yang,Qingxin Han,Yange Zhang,Jiang Wu,Xiaoliang Tang,Chunxu Dong,Weisheng Liu
Two aptasensors based on graphene oxide (GO) and molecular beacon were designed for the detection of L-tryptophan (L-Trp) using L-Trp aptamer (Trp3a-1). The fluorescein (FAM) labeled Trp3a-1 was absorbed by GO, which resulted in the fluorescence quenching , and exhibiting minimal background fluorescence. Upon the addition of L-Trp, Trp3a-1 was not absorbed quickly. This effect allows for a quantitative assay of L-Trp over the concentration range of 10–500 μM and with a detection limit of 6.84 μM. However, due to the unspecific adsorption of GO, the GO based aptasensor can׳t be applied in complex matrixes. In respect of molecular beacon based aptasensor, FRET between Trp3a-1 labeled with FAM and CS-Trp3a-1 labeled with BHQ-1(black hole quencher-1) which is partially complementary with the aptamer was used to detect L-Trp. L-Trp binding could induce the disassociation of CS-Trp3a-1, resulted in the enhancement of fluorescence in solution. With an excellent linear relationship in 10–500 μM and a detection limit of 6.97 μM in 25% serum, the aptasensor is expected to be improved for the detection of free L-Trp in other complex samples.
研究领域:
生物化学
·
分析化学
·
医学检测
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DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 27
·13058-13068
·
IF 6.97
Jiapei Gu,Xiangqian Li,Zhan Zhou,Wanqiang Liu,Kai Li,Jinwei Gao,Ying Zhao,Qianming Wang
A natural resource such as peony flower has been employed for the first time as a new carbon precursor to prepare green-emitting carbon nanodots (CDs). The emission peak is situated at 523 nm and the excitation wavelength can be extended to the visible light range (452 nm). Due to the formation of CD–MnO2 nanocomposites, the emission intensity of CDs is sharply reduced as a consequence of Förster resonance energy transfer (FRET). Moreover, glucose can be recognized due to the enzymatic conversion of glucose by glucose oxidase to generate H2O2. The MnO2 nanosheets are reduced to form Mn(II) ions, and the fluorescence of CDs can be recovered. The fluorescence intensity has been improved linearly based on the increasing concentration of glucose (0.5–250 μM) with a detection limit as low as 0.18 μM. This strategy gives a new selection of eco-friendly precursors in carbon nanomaterials and such a consecutive recognition process provides valuable insights for bio-analysis.
研究领域:
材料科学
·
生物分析
·
化学
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产品关联
DOI
PubMed
高影响因子
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