ENVIRONMENTAL POLLUTION
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2020
·
IF 6.793
Tian-Xin Zhao,Jun-Ke Wang,Lian-Ju Shen,Chun-Lan Long,Bin Liu,Yi Wei,Lin-Dong Han,Yue-Xin Wei,Sheng-De Wu,Guang-Hui Wei
Di-(2-ethylhexyl) phthalate (DEHP) is a common environmental endocrine disrupting chemical that may induce male reproductive disorders. Exposure to DEHP at a prepubertal stage could lead to prepubertal testicular injury, but the underlying mechanisms remain unclear. In this study, we exposed Sprague-Dawley rats to 0, 250, and 500 mg DEHP per kg body weight per day at the prepuberty stage from postnatal day 22 (PND 22) to PND 35 by oral gavage. Testicular injury and oxidative stress were evaluated, and the levels of 6-methyladenosine (m6A) modification and expression of modulator genes for RNA methylation were measured in testes . Furthermore, m6A modification of the important antioxidant transcription factor Nrf2 was analyzed using methylated RNA immunoprecipitation qPCR. Our results show that DEHP worsened testicular histology, decreased testosterone concentrations, downregulated expression of spermatogenesis inducers, enhanced oxidative stress, inhibited the Nrf2-mediated antioxidant pathway, and increased apoptosis in testes. Additionally, DEHP increased global levels of m6A RNA modification and altered the expression of two important RNA methylation modulator genes, FTO and YTHDC2 . Moreover, m6A modification of Nrf2 mRNA increased upon DEHP exposure. Overall, these findings link oxidative stress imbalance with epigenetic effects of DEHP toxicity and provide insight into the testicular toxicity of DEHP from the new perspective of m6A modification.
研究领域:
毒理学
·
生殖生物学
·
表观遗传学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xuemei Ma,Liu Shi,Haojie Xie,Deqi Kuang,Yetao Xu,Jiehua Ma,Jie Yang,Lizhou Sun
Fat mass and obesity-associated protein (FTO) can catalyze the demethylation of N6-methyladenosine (m6A) motif in ssDNA or RNA, which plays a crucial role in maintaining the relative stability of m6A modification. Meanwhile, dysregulation of FTO expression can lead to cancers and cardiovascular diseases. So quantitative analysis of FTO is of great significance for the research related with its biological functions and application in disease diagnosis. Herein, we propose a highly sensitive biosensor for FTO detection based on terminal deoxynucleotidyl transferase (TdT)-mediated signal amplification. To be specific, FTO can specifically demethylate the substrate probe, and the emerging double strands can be cleaved by DpnII which is a methylation-sensitive restriction endonuclease. The polymerase TdT in turn recognizes the free 3′OH and initiates polymerization to produce a mass of G-quadruplexes. Thioflavin T (ThT) can then be embedded into G-quadruplexes, producing a strong fluorescence. Experimental results manifest that the fluorescence intensity is positively correlated with the abundance of FTO. Moreover, this biosensor also has good specificity and anti-interference, and the limit of detection (LOD) is down to 30.9 fM. Therefore, our biosensor can provide an efficient strategy for FTO analysis without labeling and separation, showing great potential in clinical application.
研究领域:
生物化学
·
分子生物学
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生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Xue Zhou,Yanying Wang,Chunya Li,Tsunghsueh Wu
Amino group functionalized hydrophobic ionic liquid, 1-ethylamine hydrobromide-3-methylimidazolium hexafluorophosphate (ILPF 6 ) ionic liquid, was employed as the functional monomer to fabricate a carbon-based hole-conductor-free perovskite solar cells with high performances. The photoactive perovskite layer was facilely acquired by one-step spin-coating the precursor solution on a TiO 2 /FTO electrode surface, and subsequently annealed at an initial annealing at 60 °C for 3 min followed by annealing at 100 °C for 10 min to crystallize. The perovskite precursor solution was prepared by mixing ILPF 6 ionic liquid and methylammonium iodide with an equal molar ratio of PbI 2 . In the optimized conditions, the power conversion efficiency (PCE) was enhanced from 10.08% to 13.01% by doping ILPF 6 into the perovskite crystal film. This study demonstrates the multifaceted functions of ILPF 6 ionic liquid in improving the efficiency and stability of PSCs. First, ILPF 6 can react with PbI 2 to form perovskite and can also play a role of the controlling and crosslinking reagent to obtain large grain size and minimize defects. Secondly, ILPF 6 can improve the loading amount of perovskite crystals on the TiO 2 film. Third, ILPF 6 can promote the transport rate of the photogenerated carriers in the perovskite crystal layer. Lastly, ILPF 6 ionic liquid with its high hydrophobicity creates a moisture barrier to improve the stability of PSCs effectively. The PSCs were stored under dark in a low relative humidity atmosphere (RH of 20%) at 25 °C without sealing. After 840 h, the PSC can maintain 94% of its initial PCE under the identical intensity illumination. This study demonstrates an excellent strategy to break through the bottleneck of the traditional PSCs and the enhanced effect of ionic liquid for carbon-based PSCs.
研究领域:
材料科学
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可再生能源
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太阳能电池
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化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2018
·
IF 2.943
Yi-Yi Liu,Xin-Yu Ye,Qing-Qing An,Bing-Xin Lei,Wei Sun,Zhen-Fan Sun
The bottom-straight and top-bent dual anatase TiO 2 nanowires directly on flurine-doped tin oxide (FTO) glass substrate are successfully synthesized for the first time via a two-step solvothermal reaction, which obtains single anatase TiO 2 nanowires with the diameter of 250–450 nm and the length of ∼49 μm in the first growth step, and the slender TiO 2 nanowires with the diameter of 40–80 nm intertwining larger nanowires to form dual nanowires network in the secondary growth step. Dye-sensitized solar cell (DSSC) based on dual TiO 2 nanowires exhibits the power conversion efficiency of 5.30%, which is much higher than those of DSSCs based on the pristine single TiO 2 nanwires (2.36%) due to the twining network like dual nanowires structure maintaining excellent electron transport and simultaneously enhancing the specific surface area and light scattering ability. Moreover, by TiO 2 sol treatment, the performance of dual TiO 2 nanowires based DSSC can be further enhanced, reaching an impressive power conversion efficiency (PCE) of 7.65%.
研究领域:
材料科学
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纳米技术
·
太阳能
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Dejian Yan,Xingchen Fu,Zhichao Shang,Jikai Liu,He'an Luo
Bismuth vanadate (BiVO 4 ) has been envisioned as a promising photoanode material for photoelectrochemical (PEC) water oxidation. However, the PEC performance of BiVO 4 based photoanodes is usually dragged down by the poor charge transport and slow kinetics for an oxygen evolution reaction (OER). Here, a novel composite photoanode of Ti 3 C 2 T X /BiVO 4 was fabricated for the first time by spin coating thin Ti 3 C 2 T X (MXene) flakes onto the surface of a BiVO 4 film grown on the FTO substrate. A facile re-annealing treatment of bare BiVO 4 /FTO photoanode in argon can impressively boost the photocurrent density from 2.1 mA cm −2 up to 2.95 mA cm −2 . Experimentally, we clearly elucidated that by re-annealing, the interfacial (BiVO 4 /FTO) charge recombination could be effectively suppressed due to the better contact between the BiVO 4 film and FTO. The coating of thin Ti 3 C 2 T X flakes onto the BiVO 4 film would further increase the photocurrent density to 3.45 mA cm −2 (measured at 1.23 V vs. reversible hydrogen electrode (RHE) under 1 sun illumination), exhibiting a photoconversion efficiency of 0.78% and a surface charge separation efficiency of 73%. The outstanding PEC performance can be mainly ascribed to the coating of the thin Ti 3 C 2 T X (MXene) flakes which function as co-catalysts, facilitating the charge transfer of the photogenerated carriers and reducing the charge recombination on the BiVO 4 surface. Our findings provide a facile re-annealing strategy and a novel two-dimensional (2D) material of Ti 3 C 2 T X (MXene) as co-catalysts to improve the performance of the BiVO 4 photoanode for PEC water oxidation.
研究领域:
材料科学
·
电化学
·
可再生能源
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Yue Feng,Jiakun Shen,Zishen Lin,Zeyi Chen,Min Zhou,Xi Ma
Deoxynivalenol (DON) is a prevalent toxin causing severe liver damage through hepatocellular oxidative stress. However, the underlying mechanisms and effective therapeutic approaches remain unknown. Here, the unique role of the xenobiotic metabolism factor pregnane X receptor (PXR) in mediating DON-induced hepatocellular oxidative stress is investigated. Treatment with the PXR agonist 3-indole-propionic acid (IPA) alleviates DON-induced oxidative stress and liver injury both in vitro and in vivo. Mechanistically, it is discovered for the first time that PXR agonist IPA directly transactivates the m 6 A demethylase FTO expression, leading to site-specific demethylation and decreased abundance of YTHDC1-bound Malat1 lncRNA at single-nucleotide resolution. The diminished m 6 A modification of Malat1 lncRNA reduces its stability and augments antioxidant pathways governed by NRF2, consequently mitigating DON-induced liver injury. Furthermore, Malat1 knockout mice exhibit decreased DON-induced liver injury, emphasizing the role of Malat1 lncRNA in oxidative stress. Collectively, the findings establish that PXR-mediated m 6 A-dependent Malat1 lncRNA expression determines hepatocyte oxidative stress via m 6 A demethylase FTO, providing valuable insights into the potential mechanisms underlying DON-induced liver injury and offers potential therapeutic strategies for its treatment.
研究领域:
毒理学
·
分子生物学
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药理学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yimeng Sun,Cong Ma,Man Wu,Chunping Jia,Shilun Feng,Jianlong Zhao,Lijuan Liang
Given increasing concern regarding antibiotic environmental contamination, there is immediate need to monitor antibiotic levels to effectively control pollution. In this study, we used a photoelectrochemical aptasensor based on TiO 2 @MoS 2 spiral nanoarrays to detect chloramphenicol (CAP) in antibiotics. Nanoarrays were directly grown on fluorine-doped tin oxide (FTO) conductive glass with excellent biochemical stability, while aptamer-SH were immobilized by chemical binding on a synthetic TiO 2 @MoS 2 nanoarray. Results show that the photocurrents were reduced in the presence of photoelectrochemistry associated with specific selection of aptamer for CAP. When the measurement of the fabricated nanomaterial chip was carried out using a three-electrode system, we found a highly specific and stable detection of chloramphenicol that ranged between 0.1 pM and 1 μM, with the detection limit of 0.1 pM. In addition, we obtained satisfactory results when real sample were used to validate the potential of photoelectrochemical (PEC) aptasensor for detecting chloramphenicol content in milk. Our results demonstrate that photoelectrochemical aptasensor is conducive to the development of less toxic multifunctional nanomaterials, making the biosensor more robust and environmentally friendly. Therefore, photoelectrochemical aptasensor can be widely applied in the field of environmental monitoring.
研究领域:
环境科学
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分析化学
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纳米技术
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生物传感器
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2022
·
IF 8.035
Zhunzhun Ma,Weixin Qian,Haitao Zhang,Hongfang Ma,Qiwen Sun,Weiyong Ying
Fischer–Tropsch-to-Olefins (FTO) over iron-based catalysts is a promising process for the production of light olefins from a non-petroleum source. However, the catalytic mechanism is unclear because of the variation in the iron phase during the reduction and activation processes and the complex iron phase composition under the reaction conditions. A series of Li-promoted FeMnMgO x catalysts is synthesized using co-precipitation and impregnation methods, after which the effect of promoters on the evolution of the iron phase, chemisorption, and FTO performance are analyzed. The results demonstrate that Mg enhances dissociative CO adsorption and hydrogen adsorption, improving the FTO activity. Mg facilitates the dispersion of α-Fe 2 O 3 and suppresses the carbonization , which weakens the chain propagation, encourages the formation of short-chain hydrocarbons, and increases the selectivity of light olefins . Li also enhances dissociative CO adsorption and increases CO conversion. Moreover, Li facilitates the formation of the ε'-Fe 2.2 C phase, which reveals a higher FTS activity and better C–C coupling ability than the χ-Fe 5 C 2 phase. This results in a decrease in CO 2 and increase in light olefins . At the optimal Li loading, the FeMnMg-0.75Li sample displays the highest space time yield of light olefins at 355 g/(kg cat ·h).
研究领域:
化学工程
·
催化
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 8
·期 16
·3616-3622
·
IF 5.047
Jialian Ding,Xinru Li,Lin Zhou,Rongjie Yang,Fei Yan,Bin Su
This work reports an enzyme-free glucose sensor based on nickel nanostructures electrodeposited on a fluorine-doped tin oxide (FTO) electrode modified with a silica nanochannel membrane (SNM). The SNM consists of a high density of nanochannels vertically oriented to the electrode surface, which can spatially confine the electrodeposition of nickel nanostructures and protect them to make Ni@SNM/FTO electrodes. In alkaline media, nickel could be converted to nickel oxyhydroxide that displayed catalytic activity toward the anodic oxidation of glucose. The electrodes could thus function as enzyme-free sensors for glucose detection. Under optimal conditions, the sensors exhibited an excellent analytical performance, with an analytical sensitivity as high as 62.3 μA mM−1 cm−2, a wide detection range from 10 μM to 12 mM and a low detection limit of 0.44 μM. Furthermore, given nickel nanostructures were embedded inside the nanochannels of the SNM (with a diameter of 2–3 nm), the sensor possessed anti-fouling ability and outstanding current stability, thus allowing the direct detection of glucose in dilute blood samples.
研究领域:
分析化学
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纳米技术
·
生物传感器
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Qianhong Chen,Jinglan Cao,Yi Zhao,Ruo Yuan,Yun Xiang
The fat mass and obesity-associated protein (FTO) is vital for modulating dynamic reversible process of N6-methyladenosine (m 6 A) level in the nucleus and the abnormal expression of FTO correlates with generation and development of Alzheimer's disease, obesity and several cancers. The sensitive detection of FTO is therefore of significance for diagnosis of FTO-related diseases. In this work, on the basis of the demethylation enzyme capability of FTO and the dual Mg 2+ -dependent DNAzyme recycled signal magnification strategy, establishment of a highly sensitive electrochemical FTO biosensor is described. The target FTO molecules catalyze the demethylation of m 6 A-modifed sequences to form effective cleavage substrates for the MboI endonuclease, leading to the release of active DNAzyme sequences to trigger subsequent dual DNAzyme recycling cycles. Many methylene blue-tagged ssDNA sequences can thus be yielded via the DNAzyme amplifications and captured on the sensor electrode to generate highly amplified currents for detecting FTO in range of 0.04 pM to 10 nM with 6.11 fM detection limit. Additionally, the sensor demonstrates high selectivity and is able to be used for detecting cell lysate FTO, showing its potential for the diagnosis and study of FTO-related diseases for various application purposes.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xing-Pei Liu,Pan-Pan Li,Chang-jie Mao,He-Lin Niu,Ji-Ming Song,Bao-Kang Jin
A novel ultrasensitive photoelectrochemical aptasensing was developed to detect mucin 1 (MUC1) based on TiO 2 /CdS:Eu nanocomposite. The TiO 2 /CdS:Eu nanocomposite modified fluorine-doped tin oxide (FTO) electrode was prepared by successive ionic layer adsorption and reaction technique (SILAR) and then was employed as matrix for immobilization of SH-DNA (DNA1). After 6-mercaptohexanol (MCH) blocked unbound sites of the electrode surface, the MUC1 aptamer(DNA2) was introduced, allowing MUC1 aptamer to hybridize with DNA1. Subsequently, CdS-COOH QDs as signal amplification elements were successively labeled on the terminal of MUC1 aptamer. In this case, the target MUC1 detection was based upon the photocurrent change originated from quantities change of the signal amplification elements CdS. In the presence of MUC1, the MUC1 aptamer was specifically bound with MUC1, and meanwhile MUC1/DNA2/CdS was released from the electrode surface, resulting in significantly decreased photocurrent intensity. The novel ultrasensitive photoelectrochemical aptasensing presented a highly selective, sensitive and reproductive detection of MUC1 from 1 nM to 5 μM with a lower detection limit of 0.3 nM. At the same time, it also provided a good specificity, reproducibility, stability and promising strategy to detect a variety of other protein targets for early clinical diagnosis of different diseases.
研究领域:
生物化学
·
分析化学
·
生物传感器
·
纳米技术
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Hong-Mei Yi,Guo Yu,Zhou-Lin Lv,Hui-Fang Li,Xi Lin,Hao-Hong Li,Hui-Dong Zheng
Bio-resistive memory devices with good environmental robustness are important for the next generation of biodegradable electronic devices. In this field, the facile achievement of both resistive switching performance and environmental robustness is still challengeable. Herein, resistive switching active oxidized multi-walled carbon nanotube (O-MWCNT) has been doped into chitosan (CS) to produce twisted O-MWCNT-CS biocomposites based on strong -COO - /NH 3 + electrostatic interaction . The FTO/O-MWCNT-CS(8%)/Ag biomemorizer can exhibit good resistive switching performance with high ON/OFF ratio (10 4.51 ) and low setting voltage (0.86 V). Besides, this biomemorizer possesses good environmental robustness with thermal (200 °C) and ionizing irradiation tolerance (UV exposure for 72 h). Ag conductive filament formation and rupture account for the resistive switching behavior. Specially, the conduction pathways provided by O-MWCNT and its good radical scavenging ability are the reason for the facile realization of resistive switching performances even under large layer thickness. The facile carboxylation on MWCNT and robust O-MWCNT-CS biocompoiste together with good environmental robustness render the promising applications as green, stable and biodegradable electronic devices.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
电子学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）