MICROCHIMICA ACTA
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2024
·Vol. 191
·期 2
·1-12
·
IF 5.3
Ma Shuyan,Wei Chenjie,Bao Yuheng,Liu Yanhui,Jiang Hong,Tong Weijun,Chen Dajing,Huang Xiaojun
A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal–organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric H 2 O 2 detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter. Graphical Abstract
研究领域:
生物传感器
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纳米技术
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-8
·
IF 5.7
Quan Changyun,Quan Lingling,Wen Qinying,Yang Minghui,Li Ting
Graphene@MXene composite nanomaterials were utilized to construct an electrochemical sensor for alanine aminotransferase (ALT) detection. The combination of graphene nanosheets with MXene avoids the self-stacking of MXene and graphene, and broadens the charge transfer channel. In addition, the composite nanomaterial provides increased loading sites for pyruvate oxidase. The principle of ALT detection is a two-step enzymatic reaction. l -Alanine was initially transferred to pyruvate catalyzed by ALT. The formed pyruvate was then oxidized by pyruvate oxidase, generating H 2 O 2 . Through the detection of the generated H 2 O 2 , ALT activity was measured. The linear range of the sensor to ALT was from 5 to 400 U·L −1 with a detection limit of 0.16 U·L −1 (S/N = 3). For real sample analysis, the spiked recovery test results of ALT in serum samples were between 96.89 and 103.93% with RSD < 5%, confirming the reliability of the sensor testing results and potential clinical application of the sensor. Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
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临床化学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Changping Yang,Zhongwei Jiang,Qing Wu,Congyi Hu,Chengzhi Huang,Yuanfang Li,Shujun Zhen
Although a number of nanozymes have been developed, it is still difficult to develop single-component nanozyme with overall high multienzyme-like activities. In this study, the nanosized metal–organic frameworks (nano-MOFs) FePCN (PCN stands for porous coordination network) was synthesized by integrating zirconium and iron ions with different catalytic property on single-component MOFs and exhibited superior intrinsic multienzyme-like activities, namely oxidase-, peroxidase- and phosphatase-mimicking activity. The catalytic active sites of oxidase- and peroxidase-, and phosphatase-like activity of FePCN were Fe-centers and Zr-O clusters, respectively. Based on the intrinsic oxidase-like activity and the similarity of molecular structures between cytochrome P450 oxidase (CYP) cofactors and the organic linker in FePCN, FePCN exhibited high CYP-like activity to catalyze the oxidation of hypotensive drug 1,4-dihydropyridine (1,4-DHP) into diethyl 2,6-dimethylpyridine-3,5-dicarboxylate (DDPD) and the yield of DDPD reached over 80%. Moreover, as peroxidase- and phosphatase-mimics, FePCN was successfully applied to detecting H 2 O 2 under neutral condition and catalyzing the dephosphorylation of adenosine triphosphate (ATP), respectively. This study provides a feasible way for rational design one-component nanomaterials as multienzyme-mimics.
研究领域:
纳米技术
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材料科学
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生物化学
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药物化学
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yunfan Zhou,Xue Wei,Jun Chen,Hanzhi Xiong,Dandan Sui,Xu Chen,Wensheng Yang
Breast cancer is a malignant tumor, with various subtypes showing different behaviors. Endogenous H2O2 is an important marker of tumor progression, which makes it important to study the relationship between breast cancer subtypes and H2O2 for pathogenesis and treatment strategies, but this has rarely been reported so far. In this work, we constructed a three-dimensional (3D) electrochemiluminescence (ECL) sensing platform for the detection of H2O2 released from two typical subtypes of breast cancer cells (MCF-7 cells for luminal A-type and MDA-MB-231 cells for three negative breast cancers, TNBCs). To adequately replicate the tumor microenvironment, the peptide hydrogel was introduced as a scaffold for 3D cell culture. The titanium foam (TF) was used as a 3D electrode to better match the 3D culture substrate. N-(4-Aminobutyl)-N-ethylisoluminol (ABEI) was selected as the ECL emitter and assembled into the peptide hydrogel by hydrogen bonding and π-stacking, which resulted in a stable and homogeneous distribution of ABEI along the hydrogel fibers. Furthermore, basic amino acids were introduced to provide alkaline microenvironment for ABEI. Therefore, ABEI exhibited high ECL efficiency, resulting in a high sensitivity with an ultralow detection limit of 0.023 nM (S/N = 3) for H2O2 of the proposed ECL biosensor. MCF-7 and MDA-MB-231 cells were cultured in a 3D peptide hydrogel/ABEI/TF electrode, respectively, and endogenous H2O2 was successfully monitored. A notably significant difference of H2O2 released between MDA-MB-231 cells and MCF-7 cells without stimulation but similar extra release with stimulation were observed. These findings may help understand the physiological mechanisms behind the various subtypes and reactive oxygen species (ROS)-related treatment for breast cancer.
研究领域:
生物医学工程
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癌症生物学
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分析化学
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细胞生物学
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PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 16
·2197-2203
·
IF 2.378
Xiaoqiang Wang,Haiyan Sun,Chengkun Liu,Chao Wang
Following an inspiration from the chemistry of protein nanocages in nature, we evaluated the performance of a hemin-functionalized GroEL nanocage as peroxidase mimics for chromogenic detection. The reactivity of this biohybrid formed by detergent dialysis was found to be highly dependent on the amount of hemin encapsulated inside the GroEL central cavity. In comparison with horseradish peroxidase (HRP), the hemin–GroEL complex exhibited a higher recognition ability and reactivity towards the chromogenic substrate tested, while, similar to HRP, the peroxidase mimic followed a ping-pong catalytic mechanism. Moreover, the efficacy of hemin–GroEL for the chromogenic detection of H2O2 or glucose was demonstrated, with the detection limits determined to be 7 μM for H2O2 and 11 μM for glucose. This assay was also shown to be useful for the oxidative removal of phenolic compounds. Thus, the GroEL cage is a valuable tool for the development of analytical nano-materials and practical applications such as clinical analysis and environmental monitoring.
研究领域:
生物化学
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纳米技术
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分析化学
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环境科学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 30
·6459-6468
·
IF 5.344
Xiaodan Zhang,Yuwan Lu,Qiumeng Chen,Yuming Huang
A self-templated strategy was adopted to design hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides via the Mo or W doping of ZIF-67, and subsequent pyrolysis under an atmosphere of air at a low temperature of 450 °C. The hollow Co3O4/MO3 (M = Mo, W) mixed-metal oxides displayed tunable oxidase-like and peroxidase-like activities able to efficiently catalyze the oxidation of TMB to generate a deep blue color in the absence or presence of H2O2. Relative to that of the un-doped Co3O4, the oxidase mimic activity of the Mo-doped Co3O4 increased to 1.3 to 2.1-fold, while its peroxidase mimic activity increased to 7.1 to 19.9-fold, depending on different Mo doping amounts. The oxidase mimic activity of the W-doped Co3O4 increased to 2.1 to 2.3-fold, while its peroxidase mimic activity increased to 4.8 to 5.9-fold, depending on the different W doping amounts. The Mo- and W-doped Co3O4 nanohybrid exhibited both higher O2 and H2O2 activating capability, and their H2O2 activating capacity was superior to the O2 activating capability. Furthermore, the Mo- and W-doped Co3O4 nanohybrids exhibited similar O2 activating abilities, while the Mo-doped one displayed a higher H2O2 activating capability than the W-doped one. The discrepant peroxidase-like nature of Mo- and W-doped Co3O4 nanohybrids is likely attributed to their different catalytic mechanisms. The peroxidase-like activity of Mo-doped Co3O4 is highly related to the ˙OH free radical, while that of W-doped Co3O4 is likely ascribed to the electron transfer between TMB and H2O2. The Km values of Co3O4/MoO3 for TMB and H2O2 were 0.0352 mM and 0.134 mM, which were 3.2- and 1.9-fold lower than that of pure Co3O4, respectively. A Co3O4/MoO3-based colorimetric platform was developed for the determination of H2O2 in the 0.1–200 μM range, with a limit of detection of 0.08 μM (3σ). Based on the thiocholine (TCh) inhibition of the excellent peroxidase-like activity of Co3O4/MoO3 and the TCh generation via acetylcholinesterase (AChE) catalyzed hydrolysis of acetylthiocholine chloride (ATCh), the colorimetric platform was extended to screen AChE activity and its inhibitor.
研究领域:
纳米材料
·
酶模拟
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分析化学
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生物传感器
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催化
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PubMed
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 22
·10982-10988
·
IF 6.233
Lijian Chen,Kaifang Sun,Peipei Li,Xianzhong Fan,Jianchao Sun,Shiyun Ai
LDH nanosheets were obtained via continuous impaction and exfoliation by herring sperm DNA molecules using a constant vibration method. DNA–LDH nanohybrids were composed by electrostatic forces and they exhibited DNA-enhanced peroxidase-like activity. The morphology and structure of DNA–LDH nanohybrids were analyzed by transmission electron microscopy (TEM), selected-area electron diffraction (SAED), X-ray diffraction (XRD), and atomic force microscopy (AFM) characterization. On the basis of the high catalytic activity of DNA/CuAl–LDH nanosheets, a rapid, sensitive, and convenient approach was developed for colorimetric detection of H2O2 and blood glucose. This method can be potentially applied in medical diagnostics and biotechnology fields.
研究领域:
纳米技术
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生物传感器
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催化
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材料科学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
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