Experimental and Therapeutic Medicine
·
2022
·Vol. 23
·期 6
·1-9
·
IF 2.751
Zheng Lv,Shao Yin,Ziguan Cheng,Kekai Wang
Lenalidomide (LEN) has been reported to exert antitumor, anti‑inflammatory, anti‑angiogenic and immunomodulatory activities. However, the effects of LEN in spinal cord injury (SCI) are yet to be fully elucidated. The present study was conducted to identify whether LEN has a healing effect on SCI and to determine the underlying mechanism of action. The viability of H2O2‑stimulated PC12 cells was detected using a Cell Counting Kit‑8 assay. The viability of PC12 cells treated with LEN was examined with an MTT assay. The level of lactate dehydrogenase (LDH) was measured using an LDH assay kit, while the levels of the oxidative stress‑related factors malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase in PC12 cells were determined using commercial kits. Oxidative stress‑related proteins were examined via western blotting. PC12 cell apoptosis was observed using a TUNEL assay, while apoptosis markers and Notch signaling‑related proteins were examined via western blotting. The results of the present study demonstrated that H2O2 decreased PC12 cell viability in a dose‑dependent manner. However, treatment with LEN significantly improved the viability of H2O2‑stimulated PC12 cells and alleviated H2O2‑induced cytotoxic injury. Additionally, LEN treatment inhibited the oxidative stress and apoptosis induced by H2O2 in PC12 cells. Notch signaling‑related protein expression was downregulated after LEN treatment in H2O2‑stimulated PC12 cells. In addition, a Notch agonist reversed the effects of LEN on H2O2‑induced oxidative stress and PC12 cell apoptosis. In summary, it was demonstrated that LEN alleviated the H2O2‑induced injury of PC12 cells by blocking the Notch signaling pathway, suggesting the value of applying LEN to the treatment of SCI.
研究领域:
神经科学
·
药理学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 40
·19011-19018
·
IF 3.591
Qiuying Song,Bo Zhou,Dongyu Zhang,Haijun Chi,Hongmin Jia,Peixun Zhu,Zhiqiang Zhang,Qingtao Meng,Run Zhang
The development of well-designed fluorescence probes for the monitoring of redox homeostasis in biosystems has attracted significant research interest owing to their non-invasive and real-time detection capability in vivo. In this work, a reversible near-infrared (NIR) fluorescence probe (XC) was designed and synthesized for the monitoring of HSO3−/H2O2-regulated cycles in vivo. The XC probe contains a D–π–A–π–D conjugated structure with a reactive CC double bond in the benzopyrylium moiety. In the presence of HSO3−, a nucleophilic addition reaction with the unsaturated carbon atom of XC occurred and rearranged the structure from the positively charged benzopyrylium moiety to neutral chromene. The HSO3−-induced nucleophilic addition product (XC-SO3) can be oxidized to the native XC, resulting in the reversible responses to HSO3−/H2O2 cycles. In the presence of HSO3−, XC presented a significant decrease in the absorption and emission spectra, as well as a solution colour change from blue to colourless under physiological conditions (pH = 7.4), which allows for the detection of HSO3− by the “naked eye”. By further incubation of XC-SO3 with H2O2, the spectroscopic and colour signals were recovered. Biologically relevant species including anions, reactive oxygen species (ROS), biothiols and cations induced negligible spectroscopic responses. The detection limits of XC for HSO3− and XC-SO3 for H2O2 were calculated to be 1.02 μM and 0.84 μM, respectively. XC has several advantages such as high selectivity, reliability at physiological pH and low cytotoxicity, which enable its application in biological samples. Fluorescence imaging experiments in live adult zebrafish and live nude mice demonstrated that XC has the potential to monitor the HSO3−/H2O2-regulated redox homeostasis in biosystems. The practical applications of XC were also demonstrated by the quantitative analysis of HSO3− in white wine and sugar samples.
研究领域:
生物化学
·
分子生物学
·
分析化学
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产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2023
·Vol. 37
·期 8
·e7143
·
IF 3.9
Bolun Yu,Jingjing Yao,Haipu Li,Hui Ying Yang
The Fe 2+ -activated persulfate and H 2 O 2 (Fe 2+ /persulfate/H 2 O 2 ) system achieved 94% removal of four mixed sulfonamides with 300-s treatment and possessed excellent complementarity and stability over a wide pH (3–11) and temperature (5–65°C) range. The quenching and electron spin resonance spectroscopy results confirmed the coexistence of sulfate radicals and hydroxyl radicals in the coupled system, which were responsible for the elimination of sulfonamides under ambient conditions. The reaction rate constants of sulfate radicals and hydroxyl radicals at possible reactive sites distinguished the difference in removal ratios of four sulfonamides, according to experimental determination and density functional theory calculations. The removal ratio of sulfathiazole was higher than others because its calculated reaction rate constants of sulfate radicals and hydroxyl radicals were higher than those of sulfamerazine, sulfamethoxazole, and sulfamethazine. The finding provided a reference for investigating the removal mechanism of mixed organic pollutants in the presence of multiple free radicals.
研究领域:
环境科学
·
化学
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产品关联
DOI
高影响因子
Nanoscale
·
2024
·Vol. 16
·期 28
·13642-13653
·
IF 5.8
Ziyin Yang,Chongchao Zhang,Chengcheng Qi
Which is more suitable as a sensing material between metal single-atoms and nanoparticles? Herein, electrocatalytic behaviors of copper single-atoms (Cu SAs) and copper nanoparticles (CuNPs) toward H2O2 reduction and glucose oxidation were studied. Surprisingly, the electrocatalytic activity of Cu SAs and CuNPs showed significant differences in H2O2 reduction and glucose oxidation. Compared with CuNPs, Cu SAs exhibit outstanding activity in the electrocatalytic reduction of H2O2 but exhibit weak activity in the electrocatalytic oxidation of glucose. On the contrary, CuNPs exhibit excellent activity in the electrochemical oxidation of glucose but have very weak electrocatalytic activity towards H2O2 reduction. DFT results show that H2O2 reduction is more favourable with Cu SAs; however, the electrochemical oxidation of glucose with CuNPs requires overcoming much lower energy barriers than that with Cu SAs. This study proves that both metal single-atoms and nanoparticles are not omnipotent, which provides ideas for constructing highly active sensing materials.
研究领域:
材料科学
·
电化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Biyang Zhu,Yifan Chen,Shiyuan Chang,Huamai Qiu,Lijun You
Kinetic models and mechanism of isomaltooligosaccharides (IMOs) by hydroxyl ( OH) radicals-driven degradation in ultraviolet/hydrogen peroxide system were investigated. Electron paramagnetic resonance spectra indicated that UV radiation played an important role in the steady generation of OH radicals. The OH radicals could effectively decrease the molecular weight of IMOs and significantly oxidize hydroxy group into carbonyl and carboxy groups . Main components of IMOs were separated and identified by HPAEC-PAD and UHPLC-MS/MS methods. Degradation behaviors of IMOs were well fitted to pseudo first-order kinetics. The hydrogen abstraction by OH radicals from different C H sites at pyranose ring began a cascade reaction leading to cleavage of α-glycosidic linkage or C C bonds with formation of stable uronic/aldonic acids. Degradation rate was closely influenced by the degree of polymerization (DP) of IMOs, the initial concentrations of IMOs and H 2 O 2 . The results would pave the way for free radicals-driven degradation of polysaccharides .
研究领域:
食品科学
·
环境化学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 52
·112206-112221
·
IF 5.8
Jiang Xiaoxue,Guan Fengwei,Wang Xiaobing,Li Dong,Shi Meiqi
The degradation behavior of polyacrylamide (PAM) solution by low-temperature plasma was investigated, and the effect of some factors that might affect the degradation process was further examined. The PAM solution was treated with low-temperature plasma generated by dielectric barrier discharge (DBD) combined with H 2 O 2 and a Mn + Cu/AC composite catalyst. The optimal conditions for the oxidation degradation of a PAM solution using low-temperature plasma-H 2 O 2 -Mn + Cu/AC were determined as follows: initial concentration of 1000 mg/L, discharge voltage of 18 kV, H 2 O 2 addition of 2%, and catalyst addition of 810 mg. The results indicated that the degradation rate increased with the increase of the catalyst dosage at the same discharge time. The degradation rate of 180 min increases from 90 to 97.6% with an increase in voltage from 16 to 18 kV, and the molecular weight decreases from 2,720,204.23 to 1,370,815.54. The degradation effect caused by the change of H 2 O 2 addition was considerable compared with other factors. When the discharge time was 180 min, the degradation rate increased 26.3% with the increase of 1.6% H 2 O 2 addition. Under the optimal process conditions, the addition of the catalyst resulted in a more rapid initial decrease in the pH value of the solution compared to the system without the catalyst.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 71
·66963-66970
·
IF 3.289
Haiguan Yang,Junqi Zha,Peng Zhang,Yuhao Xiong,Linjing Su,Fanggui Ye
CoS, which was prepared using a facile solvothermal method, and characterized using various analytical techniques, was demonstrated for the first time to exhibit intrinsic peroxidase-like activity. CoS catalyzed the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2 rendering the reaction solution blue, which is the principle used for H2O2 sensing. The mechanism of the CoS catalysis was also investigated. Under optimal conditions, the linearity of H2O2 detection ranged from 0.05 to 0.8 mM (R2 = 0.9965). More importantly, using the reducibility of glutathione and the strong affinity to thiol compounds exhibited by mercury ions (Hg2+), a novel “off–on” colorimetric sensor for Hg2+ determination was designed using CoS as a peroxidase mimics. The analytical platform for Hg2+ determination was developed, ranging from 0.25 to 3 μM (R2 = 0.9965). The limit of detection was 0.1 μM. CoS exhibited several advantageous features including good chemical stability, ease of preparation, simple purification, and excellent reproducibility.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2022
·
IF 5.589
Chuanjiang Jian,Ying Wang,Huijun Liu,Zongning Yin
Although synergistic effects of photothermal therapy (PTT) and chemotherapy for cancer have been extensively investigated in previous studies, more potential strategies need to be exploited to alleviate severe adverse effects. In this study, a biotin-modified and activatable nanotheranostic system is developed. This system (BPSP/DOX-CyBA) composed of H 2 O 2 -sensitive thioketal (TK) linker, hydrophilic biotin-decorated polyethylene glycol (PEG) segment, hydrophobic polycaprolactone (PCL) segment, could self-assemble into (99 ± 1.3) nm nanoparticles and co-deliver H 2 O 2 -triggered photosensitizer CyBA and cytotoxic drugs DOX to tumor site. In vitro , DOX and CyBA could release rapidly from nanoparticles, CyBA accumulation in the mitochondria causes mitochondrial damage, leading to mitochondrial dysfunctions,while rising the level of ROS in B16F10 cells, and further to promote the micells to trigger release. CyBA could be activated into CyOH and the photothermal therapy was turn “off” into “on”. In BPSP/DOX-CyBA group, the local temperature within tumor reached 50 °C and cell apoptosis rate reached 68.6% under Laser irradiation (650 nm, 1 W/cm 2 ). Fluorescence microscopy and flow cytometry analysis further demonstrated the better uptake efficiency on B16F10 cells with biotin decoration. In a mice B16F10 tumor model, the group with co-delivery CyBA and DOX had the best tumor retention effect, the maximal local temperature increasement and the minimum tumor growth with negligible side effects, suggesting the potential of BPSP/DOX-CyBA nanopalteform that synergistic photothermal therapy and chemotherapy and mitochondria damage as an effective melanoma treatment strategy.
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Yuwei Pan,Minghua Zhou,Xiang Li,Liting Xu,Zhuoxuan Tang,Mengmeng Liu
A new modified Fenton-like process (pre-magnetization Fe 0 /H 2 O 2 ) was employed to enhance the degradation of organic pollutants and reduce reagents dosage, using azo dye of tartrazine as the model. Compared with Fe 0 /H 2 O 2 process, pre-magnetization Fe 0 /H 2 O 2 process could have 1.3–11.2 folds enhancement in the degradation rate of tartrazine at different Fe 0 dosages (0.5–4 mmol), initial pH levels (2–6.5) and H 2 O 2 dosages (0.25–2 mmol) owing to the change of Fe 0 characteristics after pre-magnetization. The contact angle of pre-magnetization Fe 0 decreased from 58.4° to 19.8° enhancing the hydrophilicity and BET increased from 0.15 m 2 /g to 1.94 m 2 /g, which improved the corrosion of Fe 0 and accelerated the generation of Fe 2+ . Similar to the case of tartrazine, pre-magnetization Fe 0 /H 2 O 2 process had remarkable improvement on many refractory contaminants. The degradation rate constant of methyl orange, orange, methylene blue, 2,4-dichlorophenol (2,4-DCP), acetylsalicylic acid, phenol and tetracycline by pre-magnetization Fe 0 /H 2 O 2 process was 5.2, 21.3, 9.2, 5.6, 12.3, 9.5 and 3 folds of that by Fe 0 /H 2 O 2 process, respectively. The “magnetic memory” of pre-magnetization Fe 0 could last 24 h which was suitable for off-site application, and the lost some or all “magnetic memory” could be recovered after second pre-magnetization. Pre-magnetization Fe 0 /H 2 O 2 is a more promising and highly efficient Fenton process since it does not require any change of the present water and wastewater treatment process , and does not need an extra energy source and complex equipment but can widen pH range and save more than 75% Fe 0 and H 2 O 2 dosages.
研究领域:
环境科学
·
化学工程
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2018
·
IF 11.698
Zhenying Jiang,Lingzhi Wang,Juying Lei,Yongdi Liu,Jinlong Zhang
The application of photo-Fenton process is restricted by the acidic pH and additional hydrogen peroxide (H 2 O 2 ). In order to overcome these drawbacks, a photo-Fenton system of CdS/rGO/Fe 2+ which can perform at natural pH with in situ -generated H 2 O 2 has been successfully developed. Highly efficient photo-Fenton degradation of phenol was achieved under the visible light irradiation via the CdS/rGO/Fe 2+ system. Experimental results showed that phenol could be degraded completely in 1 h at natural pH. The plentiful in situ generation of H 2 O 2 was further proved to act as an important role in phenol degradation with the addition of Fe 2+ . The advantages of degradation at natural pH with in situ -generated H 2 O 2 can provide a new prospective to the environmental-friendly Fenton process.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-15
·
IF 14.7
Cui Lele,Chen Bin,Chen Dongxu,He Chen,Liu Yi,Zhang Hongyi,Qiu Jian,Liu Le,Jing Wenheng,Zhang Zhenghua
The meticulous design of advanced electrocatalysts and their integration into gas diffusion electrode (GDE) architectures is emerging as a prominent research paradigm in the H 2 O 2 electrosynthesis community. However, it remains perplexing that electrocatalysts and assembled GDE frequently exhibit substantial discrepancies in H 2 O 2 selectivity during bulk electrolysis. Here, we elucidate the pivotal role of mass transfer behavior of key species (including reactants and products) beyond the intrinsic properties of the electrocatalyst in dictating electrode-scale H 2 O 2 selectivity. This tendency becomes more pronounced in high reaction rate (current density) regimes where transport limitations are intensified. By utilizing diffusion-related parameters (DRP) of GDEs (i.e., wettability and catalyst layer thickness) as probe factors, we employ both short- and long-term electrolysis in conjunction with in-situ electrochemical reflection-absorption imaging and theoretical calculations to thoroughly investigate the impact of DRP and DRP-controlled local microenvironments on O 2 and H 2 O 2 mass transfer. The mechanistic origins of diffusion-dependent conversion selectivity at the electrode scale are unveiled accordingly. The fundamental insights gained from this study underscore the necessity of architectural innovations for mainstream hydrophobic GDEs that can synchronously optimize mass transfer of reactants and products, paving the way for next-generation GDEs in gas-consuming electroreduction scenarios.
研究领域:
化学工程
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Ya Xu,Jihong Zhou,Peng Sun,Ping Li,Dongxue Han,Xiaohong Wang,Fang Chai
Artificial mimic enzymes have attracted wide attention for their superior characteristics to natural enzymes in extensive applications of diagnosis and therapy. In this paper, a nanozyme Cu-BDC@FeMo 6 was designed and fabricated by embedding polyoxometalate (NH 4 ) 3 [FeMo 6 O 18 (OH) 6 ]·6H 2 O (FeMo 6 ) into a copper-based metal–organic framework (Cu-BDC). The integration of FeMo 6 and Cu MOF based on the synergies of oxidability of FeMo 6 and oxygen-driven reversible Cu + /Cu 2+ enhanced the peroxidase mimicking activity, which accomplished the sensitive visual monitoring of H 2 O 2 and dopamine (DA). The detection of H 2 O 2 was driven by Cu-BDC@FeMo 6 catalyzing the oxidation of TMB with colorimetric evolution to blue, and consecutive monitoring DA via the reverse process of reduction of ox-TMB was further achieved. The limit of detection (LOD) of H 2 O 2 and DA were 10 μM and 2.27 μM, with excellent stability, and outstanding selectivity. The mechanism of the catalysis was further evaluated, and the generation of O 2 · - played a crucial role in the catalysis for oxidation. The logic gate design was constructed to illustrate the process and application. This work provides a feasible reference for the reasonable design of simulated enzyme in biosensor applications.
研究领域:
生物传感
·
材料科学
·
仿生催化
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）