查找使用阿拉丁产品的科研文献

Preparation and Characterization of Ha/Gel/β-TCP Microspheres Composite Porous Scaffold

Yang Zi Zhao,You Fa Wang

Being one of the three elements of tissue engineering, three-dimensional porous structure scaffold plays an important role in tissue engineering. As it not only prvovide cells for the life, but also serves as a template to guide tissue regeneration and control of organizational structure and other functions. In this study, hyaluronic acid and gelatin are successfully cross-linked by 1-ethyl- (3-dimethylaminopropyl) -carbodiimide hydrochloride (EDC) , and compound β-TCP microspheres to prepare porous hydrogel scaffolds. The microspheres were analyzed by X-ray diffraction (XRD). The scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). At the same time, the compressive strength, swelling ratio, degradation of the scaffold were tested. To assess the in vitro cell compatibility of the scaffolds, mouse L929 fibroblasts were seeded onto scaffolds for cell morphology and cell viability studies. The results showed that the pore size of the porous scaffold can be adjusted by changing the ratio of gelatin to hyaluronic acid (HA), increasing the proportion of hyaluronic acid in a certain range, pore size will be significantly increased. With the increase of the proportion of hyaluronic acid in the scaffold, the swelling ratio and the degradation rate also increased. The compressive strength of the scaffold increased with the increase of the proportion of gelatin. The appropriate ratio of β-TCP can promote cell growth and proliferation.

Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment

Junyan Chen,Wuyan Xu,Tianming Dai,Songsong Jiao,Xiang Xue,Jiayang Jiang,Siming Li,Qingqi Meng

Preparation and anti-triple-negative breast cancer cell effect of a nanoparticle for the codelivery of paclitaxel and gemcitabine

Yang Fan,Fan Zehui,Zhang Lixia,He Yanjuan,Hu Run,Xiang Jinkun,Fu Shiyang,Wang Guowei,Wang Jianlong,Tao Xiaojun,Zhang Pan

Amphiphilic polymers (HA-ANI) were prepared by grafting hyaluronic acid (HA) and 6-(2-nitroimidazole)hexylamine (ANI) and then self-assemble in water to form nanoparticles (NPs) that could be loaded with paclitaxel (PTX) and gemcitabine (GEM) by dialysis. Infrared spectroscopy and 1 H-NMR indicated the successful synthesis of HA-ANI. Three different ratios of NPs were prepared by adjusting the ratios of hydrophilic and hydrophobic materials, and the particle size decreased as the ratio of hydrophilic materials increased. When HA:ANI = 2.0:1, the nanoparticles had the smallest size distribution, good stability and near spherical shape and had high drug loading and encapsulation rates. In vitro release experiments revealed that NADPH could accelerate the drug release from NPs. Cellular uptake rate reached 86.50% at 6 h. The toxic effect of dual drug-loaded nanoparticles (P/G NPs) on MDA-MB-231 cells at 48 h was stronger than that of the free drug. The AO/EB double-staining assay revealed that a large number of late apoptotic cells appeared in the P/G NPs group, and the degree of cell damage was significantly stronger than that of the free drug group. In the cell migration assay, the 24 h-cell migration rate of the P/G NPs group was 5.99%, which was much lower than that of the free group (13.87% and 17.00%). In conclusion, MDA-MB-231 cells could effectively take up P/G NPs, while the introduction of the nano-codelivery system could significantly enhance the toxicity of the drug to MDA-MB-231 cells as well as the migration inhibition effect.

ZIF-8 and humic acid modified magnetic corn stalk biochar: An efficient, magnetically stable, and eco-friendly adsorbent for imidacloprid and thiamethoxam removal

Yong Yang,Xinxin Ma,Zifen Li,Yanru Wang,Chao Ju,Lidong Cao,Yongquan Zheng,Qingming Zhang

Neonicotinoids, ones of the most used pesticides extensively present in water environment, are hazardous to environment and human. Herein, a novel magnetic adsorbent ZIF-8-HA@MCSB is designed by modifying ZIF-8 and humic acid (HA) onto the surface of magnetic corn stalk biochar (MCSB) for removing imidacloprid (IMI) and thiamethoxam (THI) from water. MCSB has a superior adsorption performance among magnetic biochars (MBCs); the modification of ZIF-8 creating hierarchically porous structure and HA promoting the crystal growth of ZIF-8 can enhance its adsorption performance. Hence, ZIF-8-HA@MCSB exhibits the high maximum adsorption capacities for IMI (163.9 mg g −1 ) and THI (123.5 mg g −1 ). Meanwhile, the HA modification offers a protective shell for magnetic Fe 3 O 4 , which endows ZIF-8-HA@MCSB with distinguished magnetic stability. Even in pH = 2 solution, ZIF-8-HA@MCSB still possesses a saturation magnetization value of 13.07 emu g −1 , which is beneficial for its separation and reuse. Especially, ZIF-8-HA@MCSB can be repeatedly used 5 times with high removal efficiencies (over 90%). Furthermore, the low Fe (less than 0.3 mg L −1 at pH ≥ 2) and Zn (less than 1.0 mg L −1 at pH ≥ 3) leaching concentrations in a wide pH range guarantee the environmental security of ZIF-8-HA@MCSB. And a promoting effect on wheat growth enables waste ZIF-8-HA@MCSB potentially to be fertilizer for crop production, which agrees with the sustainable concept. Therefore, the advantages of having high adsorption capacities for IMI and THI, resistant to acid, and friendliness to environment indicate that ZIF-8-HA@MCSB has a significant potential for real neonicotinoids removal. Novelty Statement In this work, a novel magnetic adsorbent (ZIF-8-HA@MCSB) with the high adsorption capacities of IMI and THI is obtained by simply modifying ZIF-8 and humic acid (HA) onto the magnetic corn stalk biochar (MCSB) surface. Such a magnetic adsorbent has never been reported before. More importantly, compared with various modification methods to increase the adsorption capacity, there are few reports on concerning the acid resistance and environmental friendliness of magnetic adsorbents, especially the corresponding adsorption capacity also increases.

Consensus H3N2 HA immunogens incorporating key antigenic mutations elicit broad-spectrum neutralizing responses

Yimeng An,Meina Cai,Chenyan Zhao,Mengyi Zhang,Xi Wu,Weijin Huang,Xu Zhou,Youchun Wang

Seasonal influenza A/H3N2 poses a significant public health burden due to its rapid antigenic evolution, necessitating frequent vaccine updates. This study systematically analysed the antigenic evolution of 14 H3N2 vaccine strains (2000–2022) using pseudovirus-based neutralization assays and antigenic cartography. Vaccine strains exhibited cross-neutralization patterns, with higher reactivity among strains from adjacent years, forming six antigenic clusters. Key amino acid mutations (e.g. 144K, 189N) were identified as critical drivers of antigenic variation, with clustering-specific effects on neutralization sensitivity. Based on these findings, a consensus HA immunogen (consensus up-HA) was designed by combining mutations at key sites. This immunogen demonstrated broad-spectrum neutralization against pseudoviruses representing circulating strains from 2011 to 2024, including recent variants. These results highlight the potential of targeted HA modifications to develop broadly cross-reactive H3N2 vaccine candidate, offering broader and more durable neutralizing activity against evolving H3N2 strains.

Mesoporous CeO2 on HA Nanobundle for Selective Adsorption and Separation of Anionic Dyes

Xiaoyu Han,Meiling Zhou,Yaping Zhang,Yingru Huang,Yufeng He,Rongmin Wang

The presence of dye pollutants poses a serious environmental hazard, leading to an increasing urgency for the efficient separation of dye wastewater. In this study, by in situ growing mesoporous CeO 2 particles (CeO 2 MP) on the surface of hydroxyapatite nanobundle (HAnB), a novel adsorbent material (CeO 2 MP@HAnB) with separation function was synthesized and characterized by SEM, EDS, XPS, FT-IR, XRD, BET, and Zeta potential. Methyl blue (MeB) was selected as the representative of anionic dyes to evaluate the adsorption properties of CeO 2 MP@HAnB. It was worth noting that CeO 2 MP@HAnB exhibited excellent activity for the adsorption of MeB, reaching 86.27% adsorption rate within 0.5 min, and the adsorption rate was still 80.00% after 6 cycles. It was found that the process of MeB adsorption by CeO 2 MP@HAnB was more suitable for the Elovich and Freundlich isotherm model. In combination with kinetic, thermodynamic, BET, FT-IR, and potentiometric tests, the adsorption of CeO 2 MP@HAnB on pollutants was analyzed and summarized as a pore-filled multilayer adsorption with chemical, hydrogen bonding, and electrostatic interactions as the main mechanisms. Concurrently, CeO 2 MP@HAnB exhibited high separation efficiency and repeatability for mixtures of dyes containing MeB. The separation efficiency of MeB got to 97.97% from the mixture of MeB and methyl orange (MO). In summary, we provided a novel and efficient strategy for the synthesis of separating adsorbent. And CeO 2 MP@HAnB was expected to be applied to the efficient purification of dyes in wastewater, contributing to a safer water environment.

Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration

Kai Liu,Jiangbo Shao,Beibei Han,Jianfeng Liu,Shuai Yan,Bin Liu,Yao Liu

Peripheral nerve regeneration (PNR) represents a substantial challenge in the medical field, primarily due to the limited regenerative capacity of the peripheral nerve system (PNS). Current research efforts are focused on developing advanced medical polymer materials to enhance nerve recovery. Despite significant progress, several critical issues remain unresolved, including biocompatibility, stability, mechanical strength, controlled degradation rates, and sustained release of therapeutic agents. This study examines the utilization of hyaluronic acid hydrogels, doped with mecobalamin (MeCbl) and conductive poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), in combination with exogenous electrical stimulation (ES) for PNR of rats. The strategy utilizes the MeCbl hydrogel to create a regenerative microenvironment and provide nutritional support for nerve cells, while PEDOT:PSS facilitates enhanced electrical signal conduction. ES has been shown to promote PNR and functional recovery, thereby demonstrating considerable potential. This study aims to comprehensively analyze the synergistic effects and potential value of this combined therapeutic approach, providing novel insights and pathways for the effective PNR.

In-situ compositing HA significantly enhancing electrochemical performance of Mn-MOF anode materials

Zhi Chen,Yingnan Hua,Bing Chen,Jiwen Feng,Gang Liu,Bin Jiang

MOFs (metal–organic frameworks) have large specific surface area, abundant active sites, and tunable pore structures, making them highly attractive anode materials for lithium-ion batteries. However, their inadequate electrical conductivity and rate performance greatly hinder the further applications of MOFs in field of energy storage. In this study, we present a robust Mn-MOF/HA (humic acid) composite anode material with multilayer nano-sheet structures, synthesized via in-situ compositing HA with Mn-MOF in general hydrothermal reaction. The Mn-MOF derived from hydrothermal reaction shows poor electrochemical stability and low electrical conductivity. The incorporation of HA notably elevates electrochemical stability of materials, boosts both electrical and lithium-ion conductivity, and also modulates the microstructure to form ultrathin multilayer nanosheets by rationally adjusting the feed ratio of HA. The resulting HA 20 -Mn-MOF (with a HA feed ratio of 20 %) demonstrates significant improvements in cycling stability and rate performance, with a reversible specific capacity of 1318.7mAh/g at 0.1 A/g after 100 cycles and a substantial capacity of 657.0mAh/g even after 1000 cycles at 1 A/g. An extraordinary V-shaped capacity reversal is observed for HA 20 -Mn-MOF during cycling. Ex-situ EPR (Electron Paramagnetic Resonance) investigation reveals this capacity growth is associated with the reoxidation of active manganese during cycling, in which a notable correlation between the specific capacity and the Mn 2+ signal intensity in EPR spectra is found. These results suggest in-situ compositing HA with MOFs can be a cost-effective strategy in regulating MOFs morphology and in achieving an optimized electrochemical performance for MOFs-based electrode materials in energy storage field.

VEGF-modified PLA/HA nanocomposite fibrous membrane for cranial defect repair in rats

Yanghao Wang,Haohan Li,Cuicui Zhao,Qihan Zi,Fei He,Weizhou Wang

A major obstacle to bone tissue repair is the difficulty in establishing a rapid blood supply areas of bone defects. Vascular endothelial growth factor (VEGF)-infused tissue-engineered scaffolds offer a possible therapeutic option for these types of injuries. Their role is to accelerate angiogenesis and improve bone healing. In this study, we used electrostatic spinning and biofactor binding to construct polylactic acid (PLA)/hydroxyapatite (HA)-VEGF scaffold materials and clarify their pro-vascular role in bone defect areas for efficient bone defect repair. PLA/HA nanocomposite fibrous membranes were manufactured by selecting suitable electrostatic spinning parameters. Heparin and VEGF were bound sequentially, and then the VEGF binding and release curves of the fiber membranes were calculated. A rat cranial defect model was constructed, and PLA/HA fiber membranes bound with VEGF and unbound with VEGF were placed for treatment. Finally, we compared bone volume recovery and vascular recovery in different fibrous membrane sites. A VEGF concentration of 2.5 µg/mL achieved the maximum binding and uniform distribution of PLA/HA fibrous membranes. Extended-release experiments showed that VEGF release essentially peaked at 14 days. In vivo studies showed that PLA/HA fibrous membranes bound with VEGF significantly increased the number of vessels at the site of cranial defects, bone mineral density, bone mineral content, bone bulk density, trabecular separation, trabecular thickness, and the number of trabeculae at the site of defects in rats compared with PLA/HA fibrous membranes not bound with VEGF. VEGF-bound PLA/HA fibrous membranes demonstrate the slow release of VEGF. The VEGF binding process does not disrupt the morphology and structure of the fibrous membranes. The fibrous membranes could stimulate both osteogenesis and angiogenesis. Taken together, this research provides a new strategy for critical-sized bone defects repairing.

Synthesis of HA-SS-MP: A Prodrug With High Specificity for Cancer Cells

Bo Tao,Zong-ning Yin

Thiolated hyaluronic acid was synthesized, and a quantification method was established to determine the content of the thiol group. Three thiol compounds, 2-nitro-5-mercapto-benzoic acid (TNB), 2- mercaptonicotinic acid (2-MNA), and mercaptopyridine (MPD), were tested for their efficiency in forming adducts with hyaluronic acid (HA) through disulfide bonds. Our results showed that MPD exhibited the highest disulfide exchange efficiency when the reaction was conducted at the optimal pH value. The synthesized hyaluronic acid-SS-mercaptopurine (HA-SS-MP) was characterized by 1H-nuclear magnetic resonance spectroscopy. The HA-SS-MP micelle showed a particle size of 264.4 nm in aqueous solution. An in vitro release experiment demonstrated that the releasing rate of 6-MP from HA-SS-MP in the release medium containing glutathione (GSH) was much higher than that in the release medium without GSH. Cell uptake experiments demonstrated the CD44 targeting of hyaluronic acid. Cytotoxicity tests in mouse melanoma cells (B16F10) showed that HA-SS-MP can kill melanoma cells effectively and with lower cytotoxicity, compared with the active ingredient, 6-MP.

Hyaluronic acid (HA)-based hydrogels for full-thickness wound repairing and skin regeneration

Hong Lei,Shen Meiting,Fang Jiaxi,Wang Yezhao,Bao Zhiyuan,Bu Shizhong,Zhu Yabin

In this work, two kinds of hyaluronic acid (HA)-based hydrogels were fabricated: one is made from physical freezing-thawing of HA solution (HA1), and the other is from chemical cross-linking of HA and polysaccharide (HA2). They were applied to repair full-thickness skin defects with New Zealand rabbits as the test animals, using powder HA and cotton dress as the references. The wound starts to heal after wounds were disinfected with iodine followed by coating with HA2, HA1, HA and cotton dress (the control), respectively. They were recorded as 4 treatments (groups), HA2, HA1, HA and the control. The healing progress was followed and tested in the duration of 56 days, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, HA2 was the most promising treatment in promoting the wound healing with least serious scar formation. Immunochemistry analyses and real-time PCR tests of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-β1), exhibited that HA2 enhanced VEGF and α-SMA secretion but reduced TGF-β1 expression at early stage, which alleviated the wound inflammation, improved the skin regeneration and relieved the scar formation.

Extrusion deposition 3D printed PCL/gel/n-HA composite scaffold for bone regeneration

Chenxin Wang,Jie Liu,Mingyue Lin,Rui Zhang,Yufan Li,Yubao Li,Qin Zou