ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Min Liu,Luping Feng,Xiaoyue Zhang,Yue Hua,Yuqi Wan,Chuan Fan,Xiaoxia Lv,Hua Wang
Superwettable microwell arrays were constructed for the first time by the synergic photocatalysis of silver-doped-ZnO (Ag–ZnO) nanorods patterned on indium tin oxide (ITO) electrodes for electroanalysis of glutathione (GSH) in hela cells through the signal output of AgCl electrochemistry. The newly prepared Ag–ZnO nanorods with high photocatalysis were dispersed into an octadecyltrichlorosilane (OTS) matrix to be deposited onto ITO substrates, yielding superhydrophobic Ag–ZnO–OTS coatings. Superhydrophilic microwells were further created by the Ag–ZnO photocatalysis under UV irradiation to produce Ag–ZnO microwell arrays featuring the superwettability profile. The resulting Ag–ZnO microwell-modified ITO electrodes were employed further for electroanalysis of GSH through solid-state AgCl electrochemistry, in which the specific Ag–GSH interactions would trigger a rational decrease in the sharp AgCl peak currents at the potential approaching zero. Moreover, benefitting from the superwettability feature, the microwells on the ITO electrodes could facilitate the condensing enrichment of GSH analytes from the sample droplets, achieving improved analysis sensitivity. The as-developed electroanalysis strategy was subsequently demonstrated for the detection of GSH in hela cell supernatant with levels down to about 27.30 pM. Additionally, this synergic photocatalysis-based preparation route can be tailored for the large-scale fabrication of various array platforms with the superwettability feature for high-throughput and sensitive biological analysis.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2014
·
IF 2.212
Zhong-Ying Ma,Jia Shao,Wei-Guo Bao,Zhao-Yan Qiang,Jing-Yuan Xu
The preparation and the structure of a copper(II) complex, [Cu(4ML)Cl] (1) (H4ML = 2-acetylpyridine-4-methylthiosemicarbazone), are described. Complex 1 crystallizes in a monoclinic P21/c space group with a = 7.977(2) Å, b = 15.824(5) Å, c = 9.126(2) Å, α = γ = 90°, β = 91.974(2)°, V = 1151.26(5) Å3, Z = 4, F(0 0 0) = 620. According to X-ray crystallographic studies, each Cu(II) ion lies in a square planar coordination geometry based on the 4ML− and Cl− ligands. The complex displayed excellent inhibitory activity against various tumor cells (HeLa, HepG-2 and SGC-7901), offering lower IC50 value of 3.2 ± 0.7 μM than cisplatin (10 ± 2 μM) on HeLa cells at 48 h. Complex 1 could significantly suppress HeLa cell viability in a dose-dependent manner. Flow cytometric analysis showed that 1 induced HeLa cell apoptosis, which might be associated with cell cycle arrests at S and G2 phases. Consistent with results of DNA cleavage experiments, comet assay indicated that 1 caused severe DNA fragmentation. The production of ROS was elevated with increasing concentration of 1, suggesting that 1 was capable of promoting HeLa cell apoptosis through an oxidative DNA damage pathway.
研究领域:
药物化学
·
肿瘤学
·
生物无机化学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2020
·Vol. 11
·期 1
·1-12
·
IF 12.121
Hu Yechen,Jiang Bo,Weng Yejing,Sui Zhigang,Zhao Baofeng,Chen Yuanbo,Liu Lukuan,Wu Qiong,Liang Zhen,Zhang Lihua,Zhang Yukui
Protein N-phosphorylation plays a critical role in central metabolism and two/multicomponent signaling of prokaryotes. However, the current enrichment methods for O-phosphopeptides are not preferred for N-phosphopeptides due to the intrinsic lability of P-N bond under acidic conditions. Therefore, the effective N-phosphoproteome analysis remains challenging. Herein, bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell silica microspheres (SiO 2 @DpaZn) are tailored for rapid and effective N-phosphopeptides enrichment. Due to the coordination of phosphate groups to Zn(II), N-phosphopeptides can be effectively captured under neutral conditions. Moreover, the method is successfully applied to an E.coli and HeLa N-phosphoproteome study. These results further broaden the range of methods for the discovery of N-phosphoproteins with significant biological functions.
研究领域:
生物化学
·
蛋白质组学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2020
·Vol. 97
·期 2
·317-326
·
IF 2.721
Jingjie Tang,Dongting Huang,Fei Meng,Peng Li,Fang Peng,Junsheng Huang
Hypochlorite (ClO⁻) is of great importance either for the metabolism of living organisms or as disinfectant in daily life. However, improper concentration levels of ClO⁻ lead to serious health problems including erythrocyte damage, cardiovascular problems, neuron degeneration, lung/kidney injury and cancer. Therefore, a sensitive and selective detection method is required for the visualization and measurement of ClO⁻. In this work, a novel platinum(II) complex-based luminescent probe Pt-CHO was synthesized and utilized to detect ClO⁻. This “turn-off” probe exhibits high sensitivity, excellent selectivity, good pH stability, low limit of detection and instantaneous response ability. Moreover, the luminescent response is caused by the oxidation of aldehyde into carboxyl groups combined with the coordination of hydroxyl groups at the Pt center, which is rarely reported. The cell imaging of HeLa cells proved the considerable potential of the probe for ClO⁻ imaging in living cells.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2024
·
IF 4.1
Yongdi Liang,Tingting Pan,Qiang Li,Brian Musikavanhu,HaoChen Wang,Quanhong Ma,Zhaoli Xue,Lei Feng,Long Zhao
Viscosity is an important parameter in cells since it is closely related to a few severe human diseases. The development of fluorescent probes that can detect changes in intracellular viscosity has become an effective tool for monitoring the intracellular microenvironment. In this work, an efficient Schiff base molecular rotor, NaForm , is designed and synthesized by covalently linking a naphthol hydrazine derivative with the methyl 4-formylbenzoate group for the optical detection of viscosity changes. By increasing the viscosity of solvents, a significant enhancement of fluorescence for the probe at the yellow-green channel is observed. The probe also exhibits the advantages of high sensitivity and large Stokes shift of 128 nm. Both theoretical calculations and spectroscopic measurements suggest that the inhibition of chemical bond rotation between the hydrazine and the benzene ring leads to such a profound turn-on response. The successful applications of the probe in HeLa cells and zebrafish sufficiently demonstrate the privilege of NaForm as a practical viscosity-dependant chemosensor in aqueous samples and organisms. This chemosensor holds great promise for studying intracellular viscosity effects in various biological contexts.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2015
·Vol. 26
·期 39
·395602
·
IF 3.821
Xiaoqing Yi,Quan Zhang,Hui Dong,Dan Zhao,Jia-qi Xu,Renxi Zhuo,Feng Li
In this paper, we first synthesized a novel disulfide-coupled bis-(cyclic carbonate) (TDCSS) monomer. After ring-opening co-polymerization (ROP) of TDCSS and trimethylene carbonate (TMC) initiated by mono-methoxyl poly(ethylene glycol), the crosslinked reduction-sensitive copolymer PEG-P(TMC-co-TDCSS) was obtained via a facile one-step procedure for efficient delivery of doxorubicin (DOX) into cancer cells. To serve as controls, PEG-P(TMC-co-TDCCC), which has an analogous structure without disulfide bond, and a linear polymer PEG-PTMC were also prepared. The copolymers could self-assemble to form nano-sized micelles in an aqueous solution. As compared to PEG-PTMC, crosslinked PEG-P(TMC-co-TDCSS) and PEG-P(TMC-co-TDCCC) showed lower CMC values and thus induced a much better micelle-forming ability. In vitro release studies revealed that the drug release behavior of DOX-loaded PEG-P(TMC-co-TDCSS) micelles, which could be accelerated in the presence of 10 mM dithiothreitol (DTT), showed a similar trend in the absence of DTT compared to DOX-loaded PEG-P(TMC-co-TDCCC) micelles. Furthermore, confocal laser scanning microscopy (CLSM) indicated that DOX-loaded PEG-P(TMC-co-TDCSS) micelles were efficiently internalized into HeLa cells, releasing DOX into the cytoplasm after which the drug finally entered the nuclei, while MTT assays also demonstrated potent cytotoxic activity against HeLa cells. DOX was mainly located in the cytoplasm for reduction-insensitive PEG-P(TMC-co-TDCCC) and PEG-PTMC controls.
研究领域:
药剂学
·
生物材料
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2016
·Vol. 17
·期 3
·1600258
·
IF 3.68
Shan Zhang,Jianbin Xu,Heng Chen,Zhangfa Song,Yalan Wu,Xingyi Dai,Jie Kong
In this contribution, amphiphilic star copolymers (H40- star -PCL- a -PEG) with an H40 hyperbranched polyester core and poly(ε-caprolactone)- a -poly(ethylene glycol) copolymer arms linked with acetal groups are synthesized using ring-opening polymerization and a copper (I)-catalyzed alkyne-azide cycloaddition click reaction. The acid-cleavable acetal groups between the hydrophilic and hydrophobic segments of the arms endow the amphiphilic star copolymers with pH responsiveness. In aqueous solution, unimolecular micelles can be formed with good stability and a unique acid degradability, as is desirable for anticancer drug carriers. For the model drug of doxorubicin, the in vitro release behavior, intracellular release, and inhibition of proliferation of HeLa cells show that the acid-cleavable unimolecular micelles with anticancer activity can be dissociated in an acidic environment and efficiently internalized by HeLa cells. Due to the acid-cleavable and biodegradable nature, unimolecular micelles from amphiphilic star copolymers are promising for applications in intracellular drug delivery for cancer chemotherapy.
研究领域:
材料科学
·
药学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Jun Wang,Na Huang,Qi Peng,Xiaoyan Cheng,Wuke Li
In this paper, the luminescent temperature/pH dual-responsive nanoparticles based on lanthanide-polyoxometalates were fabricated as a controllable drug delivery system. In the drug delivery carrier, the mesoporous silica acted as the core, and the poly (N-isopropylacrylamide-co-methacrylic acid) and lanthanide-polyoxometalates served as the dual-responsive shell and luminescent labeling, respectively. Composite hydrogels exhibited red luminescence under UV irradiation, which was visible to the naked eye. The releases of doxorubicin hydrochloride (DOX) from the composites were temperature/pH-dependent. DOX can be quickly released in an acidic environment and at high temperatures. Moreover, the drug delivery carrier showed minimal toxicity to HeLa cells and exhibited good biocompatibility. However, DOX loaded drug-delivery system showed the significant toxicity to HeLa cancer cells in vitro. Cell imaging has shown that lanthanide polyoxometalates composites can be used as bioimaging materials for cell monitoring. As a result, the drug delivery systems based on lanthanide-polyoxometalates have potential applications in the field of biomedical science.
研究领域:
生物医学
·
材料科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2017
·
IF 3.473
Qiao Hu,Caiqin Qin,Lei Huang,Hongying Wang,Qiang Liu,Lintao Zeng
Hypochlorous acid (HOCl)/hypochlorite (ClO − ), an endogenous reactive oxygen species (ROS), plays an important role in a variety of physiological and pathological processes. Herein, a ratiometric fluorescent probe ( BIP ) for ClO − was prepared, which exhibited a noticeable color change from purple-red to colorless along with a remarkable fluorescence change from bright red to blue in response to ClO − . The probe could quantitatively determine the concentrations of ClO − with large Stokes shift (107 nm), excellent selectivity, fast response, as well as low limit of detection (182 nM). NMR and mass spectral analysis demonstrated that ClO − triggered the oxidative cleavage of the bridge double bond in BIP , resulting in significant changes in color and fluorescence. In particular, BIP was a specific mitochondria-targeted fluorescent probe with excellent photostability, biocompatibility and cell membrane permeability. BIP was successfully used to image intracellular ClO − and its fluctuation in living HeLa cells (human cervical cancer). Furthermore, the probe was employed to visualize the ClO − mediated redox equilibrium within living cells by a ratiometric fluorescence imaging manner. Therefore, BIP has a great potential application for the detecting HOCl, which also offers an effective tool for the investigation of some HOCl related biological processes.
研究领域:
生物化学
·
医学
·
细胞生物学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yingying Zhang,Fanqiang Bu,Yanliang Zhao,Bing Zhao,Liyan Wang,Bo Song
Intracellular pH (pHi) plays an essential role in organelles . Fluorescent probe combined with fluorescence imaging analytical approach has been used for detection pH fluctuation due to high sensitivity and good photostability. Herein, a benzoindole-based colorimetric and naked-eye hemicyanine fluorescent probe 2,3-trimethy-3-[2,4-(dihyoxyl-4-yl)vinyl]-3 H -benzo[e]indole ( BiDD ) was developed in one step. Upon the decreasing of pH, BiDD exhibited strong a pH-dependent characteristic with p K a 4.98 and responded linearly within the pH range of 4.4–6.2. BiDD also showed high sensitivity and selectivity , colorimetric and fluorometric dual-modal response, high photostability, low cytotoxicity as well as good cell membrane permeability. More importantly, the probe was applied to sense and visualize the pH fluctuations in HeLa cells successfully by the fluorescence confocal microscope .
研究领域:
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Bibo Zhang,Haiyang Liu,Fengxu Wu,GeFei Hao,Yuzong Chen,Chunyan Tan,Ying Tan,Yuyang Jiang
A new and dual-response fluorescent probe (QLBM) based on quinoline and benzimidazole groups has been designed and developed for recognition of copper(II) ion (Cu 2+ ) and iron(III) ion (Fe 3+ ) simultaneously. This sensor displays a favorable selectivity for Cu 2+ and Fe 3+ ions over a range of other common metal cations in 100% aqueous medium (50 mM Tris buffer, pH 7.2), leading to prominent fluorescence on-off. The detection limits were 1.35 × 10 −7 M for Cu 2+ and 1.24 × 10 −7 M for Fe 3+ respectively. This dual-response fluorescent probe for Cu 2+ and Fe 3+ was distinguished by further reduction of the ascorbic acid. It is reported for the first time to use ascorbic acid to discriminate Cu 2+ and Fe 3+ by fluorescent probe technology. The recognizing mechanism of QLBM toward Cu 2+ and Fe 3+ has been investigated in detail by NMR, HR-MS and density functional theory (DFT) calculations. Moreover, further application of the sensor in fluorescence imaging was studied in HeLa cells due to the low cytotoxicity and good photophysical properties, suggesting that QLBM could be used as a fluorescent sensor for tracking Cu 2+ and Fe 3+ in cells.
研究领域:
分析化学
·
生物医学工程
·
细胞生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xiao Fang Zhang,Na Li,Yu Ling,Li Tang,Nian Bing Li,Hong Qun Luo
As one of the DNA damage repair enzymes, uracil-DNA glycosylase (UDG) plays an important role in maintaining genomic integrity. By linked bridge hybridizing-induced split G-quadruplex (SQ), we demonstrate here the construction of a simple and sensitive DNA machine for the detection of UDG activity. The satisfactory split G-quadruplex sequences (SQS) were successfully selected to be the ingredients of SQ formation. In this work, UDG recognized and removed the uracil bases from the stem of hairpin DNA (HP). And then, HP with a low melting temperature hybridized with designed SQS, forming a three-way DNA structure with an SQ. With the addition of Thioflavin T, a dramatical enhancement of fluorescence intensity was presented due to the G-quadruplex/Thioflavin T complex formation. The detection limit was as low as 7.8 × 10 −3 U/mL. And we also successfully investigated the performance of UDG activity in the HeLa cell lysate. This optical DNA machine with the merits of being simple, rapid, and economical was flexibly suitable for not only active UDG assay but also diverse target detection by adjusting the recognition region of the HP.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）