OPTICS EXPRESS
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2020
·Vol. 28
·期 8
·12233-12242
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IF 3.669
Xiaoyu Shi,Kun Ge,Jun-Hua Tong,Tianrui Zhai
Low-cost and miniaturized biosensors are key factors leading to the possibility of portable and integrated biomedical system, which play an important role in clinical medicine and life sciences. Random lasers with simple structures provide opportunities for detecting biomolecules. Here, low-cost biosensors on fiber facet for label-free detecting biomolecules are demonstrated based on a plasmonic random laser. The random laser is achieved resorting to a self-assembled plasmonic scattering structure of Ag nanoparticles and polymer film on fiber facet. Refractive index sensitivity and near-surface sensitivity of the biosensor are systematically studied. Furthermore, the biosensor is used to detect IgG through specific binding to protein A, exhibiting the detecting limit of 0.68 nM. It is believed that this work may promote the applications of a plasmonic random laser bio-probe in portable or integrated medical diagnostic platforms, and provide fundamental understanding for the life science.
研究领域:
生物医学工程
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纳米技术
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光学传感
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DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
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2025
·
IF 2.3
Chang Guanglei,Yang Miaomiao,Xu Zhaobin,Wang Xinling,Li Wenyao,He Meixia,Zhang Jintao,Xu Yuanzhi,Wang Lulu,Zhang Liguo
The performance of chromatographic media affects the efficiency of purification. In this study, based on a T-type microfluidic droplet generation system, agarose microspheres with large pore size and high load were prepared by adjusting the viscosity of a 4% agarose solution to 70 mPa·s at high temperature. Optical microscopy revealed that the self-made agarose microspheres had a good spherical structure with an average particle size of 82.81 μm and a CV value of 0.0576. Subsequently, the stability and mechanical strength of the self-made agarose microspheres were improved by cross-linking and activation with epichlorohydrin. After the cross-linking and activation, the agarose microspheres were grafted with Protein A to make it have specific adsorption capacity for IgG. The adsorption capacity of the self-made agarose microspheres grafted with Protein A for IgG was 87 mg/g. In the complex serum environment, the agarose microspheres grafted with Protein A still maintained good IgG adsorption capacity and selectivity, demonstrating their potential in practical applications. This study demonstrates the great potential of microfluidic technology in the design and preparation of bio-separation media. By precisely controlling the particle size and pore size of the microspheres, the purification efficiency and selectivity can be significantly improved, providing a new solution for the efficient purification of bio-products. Graphical abstract
研究领域:
生物分离
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生物技术
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化学工程
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Lidong Pang,Chen Chen,Shihang Li,Jia Shi,Yue Su,Xinyan Yang,Yujun Jiang
Whey protein isolate (WPI) is an important food ingredient, but its high allergenicity limit its application. Recently, metal–phenolic networks (MPNs) have been shown to be effective in modifying proteins. The aim of this study was to evaluate the effects of MPNs formed from (−)-epigallocatechin-3-gallate (EGCG) and Fe 3+ on the structure, antibody-binding capacity, and functional properties of WPI. The results showed that MPN modification significantly reduced the antibody-binding capacity of WPI with immunoglobulin E and IgG binding reduced by 76.40 % and 84.74 %, respectively. Multispectroscopy and liquid chromatography–tandem mass spectrometry analyses suggest that this reduction is due to the destruction of conformational epitopes and the masking of linear epitopes by MPNs. Additionally, the introduction of phenolic hydroxyl groups through MPN modification led to conformational unfolding of WPI and reduced surface hydrophobicity, thereby enhancing its antioxidant activity, hypoglycemic ability, and foaming properties. These improvements were more pronounced at higher doses. Specifically, at a dosage of 96 mM, the total antioxidant capacity is 44.01 % and the foaming properties are 104 %. This study provides new insights into the modification of WPI and offers a promising strategy for developing hypoallergenic foods products.
研究领域:
食品科学
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过敏研究
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蛋白质化学
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生物功能特性
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Qi Wang,Bo-Tao Wang
A graphene oxide/silver coated polymer cladding silica fiber optical surface plasmon resonance (SPR) human IgG detection biosensor with a high sensitivity and a low limit of detection is presented. Due to the graphene oxide (GO), the intensity of the confined electric field surrounding the sensing layer can be enhanced, the sensitivity of refractive index (RI) of the graphene oxide/silver SPR sensor reached 3311 nm/RIU in the RI range of 1.3334–1.3731 compared with 2875 nm/RIU in the range of 1.3328–1.3739 of the silver film SPR sensor. Afterwards, SPR optical fiber biosensor was developed based on graphene oxide/silver coated optical fiber when staphylococcal protein A and goat anti-human IgG were immobilized on the surface of the graphene oxide film. The sensor can be used to detect different concentrations of human IgG, a high sensitivity of 0.4985 nm/(μg/mL) and a low limit of detection 0.04 μg/mL were observed. Therefore, the graphene oxide/silver film SPR human IgG detection biosensor with high sensitivity and low limit of detection shows a great promise for the future biochemical and biomedical application.
研究领域:
生物传感技术
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医学检测
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光学工程
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2015
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IF 6.409
Yixi Liu,Le Liu,Yonghong He,Qinghua He,Hui Ma
Quantum dots encoded microbeads have various advantages such as large surface area, superb optical properties and the ability of multiplexing. Molecularly imprinted polymer that can mimic the natural recognition entities has high affinity and selectivity for the specific analyte. Here, the concept of utilizing the quantum dots encoded microbeads as the supporting material and the polydopamine as the functional monomer to form the core–shell molecular imprinted polymer was proposed for the first time. The resulted imprinted polymer can provide various merits: polymerization can complete in aqueous environment ; fabrication procedure is facile and universal; the obvious economic advantage; the thickness of the imprinting layer is highly controllable; polydopamine coating can improve the biocompatibility of the quantum dot encoded microbeads. The rabbit IgG binding and flow cytometer experiment result showed the distinct advantages of this strategy: cost-saving, facile and fast preparation procedure. Most importantly, the ability for the multichannel detection, which makes the imprinted polydopamine modified encoded-beads very attractive in protein pre-concentration, recognition, separation and biosensing.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2016
·
IF 7.145
Zhen Lei,Jiaxue Gao,Xia Liu,Dianjun Liu,Zhenxin Wang
We developed a three-dimensional (3D) polymer-brush substrate for protein and peptide microarray fabrication, and this substrate was facilely prepared by copolymerization of glycidyl methacrylate (GMA) and 2-hydroxyethyl methacrylate (HEMA) monomers via surface-initiated atom transfer radical polymerization (SI-ATRP) on a glass slide. The performance of obtained poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) (P(GMA-HEMA)) brush substrate was assessed by binding of human IgG with rabbit antihuman IgG antibodies on a protein microarray and by the determination of matrix metalloproteinase (MMP) activities on a peptide microarray. The P(GMA-HEMA) brush substrate exhibited higher immobilization capacities for proteins and peptides than those of a two-dimensional (2D) planar epoxy slide. Furthermore, the sensitivity of the P(GMA-HEMA) brush-based microarray on rabbit antihuman IgG antibody detection was much higher than that of its 2D counterpart. The enzyme activities of MMPs were determined specifically with a low detection limit of 6.0 pg mL–1 for MMP-2 and 5.7 pg mL–1 for MMP-9. By taking advantage of the biocompatibility of PHEMA, the P(GMA-HEMA) brush-based peptide microarray was also employed to evaluate the secretion of MMP-2 and MMP-9 by cells cultured off the chip or directly on the chip, and satisfactory results were obtained.
研究领域:
生物材料
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生物化学
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生物技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
Parasites & Vectors
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2021
·Vol. 14
·期 1
·1-11
·
IF 3.876
Li Jian,Ding Jing,Liu Xiao-Lei,Tang Bin,Bai Xue,Wang Yang,Qiao Wei-Dong,Liu Ming-Yuan,Wang Xue-Lin
Background Trichinella spiralis is a zoonotic food-borne parasite. A disease caused by infection with T. spiralis is called trichinellosis in humans. It is important to investigate the epidemic situation and the surveillance of herds and then prevent infection in humans. Therefore, this study is to develop a rapid and sensitive diagnostic method for on-site test in domestic and wild animals.Methods Upconverting phosphor nanoparticles (UCNPs), an excellent optical label, were conjugated with the excretory-secretory (ES) antigens from T. spiralis muscle larvae (ML) or goat anti-rabbit IgG, and a lateral flow (LF) assay based on these probes (UCNPs-ES/goat anti-rabbit IgG) was developed for the rapid and sensitive detection of anti-T. spiralis IgG antibodies in pig serum. The assay is named the UPT-LF-ES assay. In addition, the probes were characterized, and the assay was optimized. A cut-off threshold of the assay was also identified by using 169 known negative pig samples. Performance of the assay to T. spiralis with different infective numbers, cross-reactivity with other parasitic infections, the single-blinded experiment, and coincidence were evaluated with the assay.Results The UPT-LF-ES assay was successfully constructed and optimized based on the probes of UCNPs-ES/goat anti-rabbit IgG. In the pigs infected with 100, 1000, and 10,000 ML, positive results were first presented at 35 days post-infection (dpi), 30 dpi, and 25 dpi, respectively. The assay had no cross-reaction with other parasitic infections. A single-blinded experiment indicated that the sensitivity and specificity of the UPT-LF-ES assay were 100% and 100%, respectively, the area under the receiver operating characteristic (ROC) curve was 1.000. In addition, the value detected by the UPT-LF-ES assay was significantly different between positive and negative samples. Moreover, compared with the “gold standard” magnetic stirrer method, the coincidence rate of the UPT-LF-ES assay was 87.27%, and the kappa (K) coefficient was 0.7454, showing a substantial agreement.Conclusion sThe UPT-LF-ES assay is a useful point-of-care test (POCT) with T. spiralis in the detection of pig, which contributes to preventing human trichinellosis.Graphical Abstract
研究领域:
寄生虫学
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兽医学
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诊断技术和方法
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2025
·
IF 1.8
Yongsheng Chang,Xiane Guo,Xiuzhen Ge,Xuexi Yu,Shujun Ma,Canyu Liu,Yunwei Lou,Tingmin Chang,Yun Zhang
Traditional immunoassays for Staphylococcus aureus (S. aureus) suffer from significant limitations, particularly owing to cross-reactivity with protein G-producing Streptococcus species. To address this issue, here is reported an innovative colorimetric strategy that depended on the unique binding characteristics of guinea pig immunoglobulin G (IgG) toward staphylococcal protein A (SPA), a highly expressed epitope on S. aureus. Unlike conventional monoclonal or polyclonal antibodies, guinea pig IgG exhibits high specificity for SPA, while demonstrating negligible affinity for protein G. The method also integrated horseradish peroxidase (HRP)-conjugated chicken anti-protein A immunoglobulin Y (IgY) (HRP–IgY) in a sandwich immunoassay format, enabling the catalytic oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and hydrogen peroxide. The signal intensity correlated linearly with S. aureus concentration over a dynamic range of 7.8 × 102 CFU/mL–1.0 × 105 CFU/mL, achieving a detection limit of 6.9 × 102 CFU/mL. Notably, the assay demonstrated exceptional specificity, with no cross-reactivity with other common foodborne pathogens. Compared with mainstream methods, this assay completed the entire analytical process within 90 min-shorter than polymerase chain reaction (PCR) and traditional enzyme-linked immunosorbent assay (ELISA). It eliminated the need for complex DNA extraction or strict anti-cross-contamination controls and maintained stable performance in real samples. This advantage outperforms PCR (often susceptible to matrix inhibitors) and ELISA (typically exhibits reduced sensitivity in complex matrices). By offering significant improvements over conventional techniques, this strategy holds great promise for enhancing the efficiency and reliability of S. aureus detection in practical applications.
研究领域:
免疫学
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生物传感器
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食品安全
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微生物学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Jiali Gu,Yan Zhang,Hongfei Tong,Yang Liu,Laiyu Sun,Yajun Wang,Li Xiao
In the present work, mixed-mode chromatography adsorbents grafted with dextran of different molecular weight were prepared. Compared to non-grafted adsorbent (BA-6B), the morphology of dextran-grafted adsorbents (BA-6B-Ts) did not change significantly, while the water content ( ω ), porosity ( P ) and pore volume ( V ) decreased. Static adsorption, salt tolerance and dynamic adsorption behaviors of adsorbents BA-6B-Ts against BSA/IgG were studied. Compared to adsorbent BA-6B, adsorbents BA-6B-Ts had higher saturated adsorption capacity ( Q m ) toward BSA/IgG, especially for BA-6B-T20, the Q m values toward BSA and IgG increased by 32.8% and 39.5%, respectively. Adsorbents BA-6B-Ts showed enhanced salt tolerance toward BSA, and relatively weaker salt tolerance toward IgG. The relative adsorption capacity of BA-6B-T20 was 66.7% at 0.1 mol/L NaCl and 39.2% at 0.5 mol/L NaCl, higher than those of BA-6B. Moreover, for both BSA and IgG, the effective pore diffusivity ( D e ) of BA-6B-Ts was higher than that of BA-6B. D e values of BA-6B-T20 toward BSA and IgG increased by 77% and 139% compared to BA-6B, respectively. Dynamic binding capacities (DBCs) of BA-6B-Ts were also improved. DBC of BSA and IgG on BA-6B-T20 were 25.6% and 46.5% higher than those on BA-6B. The possible mechanisms for the above difference in adsorption behavior of dextran-grafted adsorbents toward BSA/IgG were discussed, providing some insights into protein adsorption and mass transport for dextran-grafted MMC resins.
研究领域:
生物化学
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分离科学
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蛋白质组学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-10
·
IF 5.833
Yang Hongmei,Wang Yafen,Tang Jing,Wang Fang,Chen Zilin
A promising electrochemical strategy for assay of N6-methyladenosine (m6A)/N6-methyladenine (6mA) in RNA/DNA is proposed. The key of this strategy is the end-labeling of nucleic acid, which makes it possible to detect methylation level in unknown sequence. Firstly, the end of m6A-RNA or 6mA-DNA was labeled with sulfhydryl group through T4 polynucleotide kinase (T4 PNK) and then directly assembled on a gold nanoparticle–modified glassy carbon electrode (AuNPs/GCE). Secondly, methylation sites in RNA/DNA were specifically recognized by anti-m6A-antibody, and then, horseradish peroxidase–labeled goat anti-rabbit IgG (HRP-IgG) was further conjugated on the antibody. Thirdly, HRP-IgG catalyzed the hydroquinone oxidation reaction to generate amplified current signal which correlates with the amount of m6A/6mA in nucleic acid. This method showed a wide linear range from 0.0001 to 10 nM for m6A-RNA, 0.001 to 100 nM for 6mA-dsDNA, and 0.0001 to 10 nM for 6mA-ssDNA. The method was successfully applied to detection of m6A/6mA in RNA/DNA from HeLa cells and E. coli cells and validation of the decrease of m6A-RNA in HeLa cells after treatment with FTO protein. Graphical abstract
研究领域:
电化学
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生物化学
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Min Chen,Zhen Song,Rui Han,Yang Li,Xiliang Luo
The nonspecific adsorption and accumulation of biomolecules on electrode interfaces remains a challenge for sensitive and accurate detection of disease markers in complex biological media, and it is highly desired to develop antifouling biosensors capable of assaying targets in complicated real liquids. Herein, an efficient and simple antifouling biosensor was constructed based on a self-designed Y-shaped peptide. The Y-shaped peptide was designed with two branches: one branch with the peptide sequence of EKEKEKE for antifouling, and the other branch with the peptide sequence of HWRGWVA for recognizing of human IgG. Under optimized experimental conditions, electrodes modified with Y-shaped peptides exhibited excellent antifouling and electrochemical sensing performances. The developed biosensor was able to effectively resist biofouling in various protein solutions and even serum samples, and the linear range of the biosensor for human IgG detection was from 100 pg mL −1 to 10 μg mL −1 , with a relatively low limit of detection of 32 pg mL −1 (S/N = 3). The antifouling biosensor possesses the capability of assaying human IgG in real serum samples, and this strategy of developing low fouling biosensors based on Y-shaped peptides can be readily expanded to the construction of other biosensing systems for different targets.
研究领域:
生物传感器
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电化学
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肽工程
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分析化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
He Dongxia,Liu Qi,Wang Lei,Han Qinqin,Zhang Jinyang,Li Chao,Song Yuzhu
A novel strategy integrating Brevinin-1BW (BW)-functionalized magnetic bead-based separation with a colorimetric immunoassay was developed to concentrate and enrich Staphylococcus aureus ( S. aureus ) from sample matrices using magnetic beads functionalized with antimicrobial peptide BW (MBs-BW) as carriers. To ensure the selectivity of the method, horseradish peroxidase (HRP)-labeled porcine IgG (HRP-porcine IgG) was used as a molecular recognition reagent and signal amplification probe. MBs-BW/ S. aureus /HRP-porcine IgG probe sandwich complex was successfully obtained. HRP chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) was used to realize the chromogenic reaction. Finally, S. aureus was quantitatively analyzed based on the color signal generated. Under the optimal conditions, the assay showed a response in the concentration range of 1.0 × 10 2 –1.0 × 10 7 CFU/mL of S. aureus , and the detection limit was as low as 60 CFU/mL. This method does not require complicated sample pretreatment, and the operation process is fast and simple, which can directly complete the whole process of bacterial enrichment and detection. The recovery of this strategy in different types of spiked samples reached 86.8–97.8%, and the analytical results of this strategy were highly consistent with those of the conventional plate counting method. This strategy provides a new way for the rapid detection of S. aureus in complex samples. Graphical
研究领域:
微生物学
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）