APPLIED CATALYSIS B-ENVIRONMENTAL
·
2022
·
IF 24.319
Minxian Zhang,Xuecong Lin,Ziran Yi,Xiang Xu,Jingling Yang,Mingshan Zhu
Through visible light driven ligand-to-metal charge transfer (LMCT) with organic pollutant-semiconductor is a new method to promote reactive oxygen species (ROS) generation in water treatment. This study is the first time to use ciprofloxacin (CIP) pollutants induced LMCT on ZnO mesocrystal with rich oxygen vacancies (M-ZnO-OV) to improve ROS generation for CIP self-degradation. The results show an obviously increased intensity of HO • and O 2 •− through the LMCT process, leading to the optimal kinetic rate constant of 0.043 min –1 for CIP degradation, which is 7.5 times that of ZnO non-LMCT process. The presence of oxygen vacancies (OVs) in M-ZnO-OV leads to an electron-deficient Zn atom nearby OVs, promoting the formation of LMCT complex, and then resulting in rapid electron transfer from OVs to oxygen in water to generate ROS. The present investigation provides a new OVs-enhanced LMCT process to improve the concentration of ROS for water treatments.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yue Wang,Wenchang Zhao,Ruixia Gao,Sameer Hussain,Yi Hao,Jiahao Tian,Shihui Chen,Yunhao Feng,Yubo Zhao,Yuyao Qu
17β-estradiol (E2), as one of the pharmaceutical and personal care product , frequently contaminates environmental water as estrogen pollutant and possesses great risk to human survival as well as the sustainable development of the ecosystem. Herein, to achieve an effective adsorbent system for the selective removal of E2 from the environmental water, Fe 3 O 4 nanoparticles are subjected to chemical etching to reduce the overall mass and then employed as carriers to prepare a novel type of lightweight daisy-like magnetic molecularly imprinted polymers (LD-MMIPs) adopting template immobilization strategy. The LD-MMIPs based etched magnetic nanoparticles not only exhibit light mass but also have plentiful imprinted sites in the etched channels, which significantly increases the adsorption capacity for E2. The daisy-like LD-MMIPs own strong magnetic responsiveness, well crystallinity , fast binding kinetics, high adsorption amount, and excellent selectivity . Moreover, combining with HPLC, the LD-MMIPs as adsorbents have been successfully used to specifically recognize and detect trace E2 in environmental water. Thus, the proposed LD-MMIPs with high adsorption capacity hold great potential in monitoring water pollution . Additionally, this work also provides an alternative strategy for improving the adsorption capacity of magnetic molecularly imprinted polymers through a convenient chemical etching technology .
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POLYMER BULLETIN
·
2021
·Vol. 79
·期 10
·8617-8631
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IF 2.87
Wang Shihai,Zhou Yu,Tao Wangwang,Liu Jingyi,Chen Hongxiang,Zhao Zuyi
In order to obtain polyurethane micelles with targeting property and excellent stability, Fe 3 O 4 -modified magnetic amphiphilic polyurethane (MAPU) was successfully synthesized by grafting amphiphilic polyurethane to the surface of Fe 3 O 4 nanoparticles. The structure of MAPU was confirmed by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy ( 1 H NMR), gel permeation chromatography and thermogravimetric analyzer. MAPU could form micelles with the particle size of less than 45 nm, and the micelles showed excellent stability of anti-dilution and storage, determined by dynamic light scattering. MAPU micelles had superparamagnetic property, and magnetically targeted property in HeLa cells measured by vibrating sample magnetometer (VSM) and Prussian blue staining method, respectively. Methyltetrazolium (MTT) assay experiment indicated that MAPU micelles were almost non-cytotoxic. In addition, MAPU micelles could encapsulate hydrophobic model drugs such as Indometacin (IMC) and had a release-sustained effect for IMC.
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PHARMAZIE
·
2021
·Vol. 76
·期 10
·499-502
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IF 1.267
Tao Lijun,Chen Lina,Pei Yanmin,Ma Ping,Xu Qingbin,Wang Jialing,Yu Yang,Cheng Jiang,Zhou Ru
The aim of this study was to investigate the vasodilatory effects of betaine, an alkaloid isolated from Lycium barbarum, on isolated pulmonary artery rings in rats and its possible mechanisms. Pulmonary vessels of normal Sprague–Dawley rats were isolated and pre-contracted
using norepinephrine. Then, betaine was cumulatively added in differing concentrations (0.02-0.14 mg/mL), and the tension curve was observed and recorded. Changes in the tension of the pulmonary artery rings with an intact endothelium and a dissected endothelium were recorded. The interactions
among betaine and NG-nitro-L-arginine methyl ester, indomethacin, 4-aminopyridine, barium chloride, and glibenclamide were evaluated. The experimental results show that betaine can relax the pulmonary artery rings pre-contracted by norepinephrine. Furthermore, pre-incubation with NG-nitro-L-arginine
methyl ester and indomethacin did not inhibit betaine vasodilation, demonstrating that vasodilation by betaine is endothelium-dependent. Additionally, pretreatment of pulmonary artery rings with 4-aminopyridine and glibenclamide had no effect on betaine. However, pretreatment of pulmonary
artery rings with barium chloride attenuated the effects of betaine. In conclusion, the vasodilatory effects of betaine on pulmonary artery rings is associated with inward rectifier potassium channels.
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Frontiers in Pharmacology
·
2021
·
IF 5.811
Feng Huixia,Chen Guilin,Zhang Yongli,Guo Mingquan
Podophyllum sinense (P. sinense) has been used as a traditional herbal medicine for ages due to its extensive pharmaceutical activities, including antiproliferative, anti-inflammatory, antiviral, insecticidal effects, etc. Nevertheless, the specific bioactive constituents responsible for its antiproliferative, anti-inflammatory, and antiviral activities remain elusive, owing to its complicated and diversified chemical components. In order to explore these specific bioactive components and their potential interaction targets, affinity ultrafiltration with multiple drug targets coupled with high performance liquid chromatography/mass spectrometry (UF–HPLC/MS) strategy was developed to rapidly screen out and identify bioactive compounds against four well-known drug targets that are correlated to the application of P. sinense as a traditional medicine, namely, Topo I, Topo II, COX-2, and ACE2. As a result, 7, 10, 6, and 7 phytochemicals were screened out as the potential Topo I, Topo II, COX-2, and ACE2 ligands, respectively. Further confirmation of these potential bioactive components with antiproliferative and COX-2 inhibitory assays in vitro was also implemented. Herein, diphyllin and podophyllotoxin with higher EF values demonstrated higher inhibitory rates against A549 and HT-29 cells as compared with those of 5-FU and etoposide. The IC50 values of diphyllin were calculated at 6.46 ± 1.79 and 30.73 ± 0.56 μM on A549 and HT-29 cells, respectively. Moreover, diphyllin exhibited good COX-2 inhibitory activity with the IC50 value at 1.29 ± 0.14 μM, whereas indomethacin was 1.22 ± 0.08 μM. In addition, those representative constituents with good affinity on Topo I, Topo II, COX-2, or ACE2, such as diphyllin, podophyllotoxin, and diphyllin O-glucoside, were further validated with molecular docking analysis. Above all, the integrated method of UF–HPLC/MS with multiple drug targets rapidly singled out multi-target bioactive components and partly elucidated their action mechanisms regarding its multiple pharmacological effects from P. sinense, which could provide valuable information about its further development for the new multi-target drug discovery from natural medicines.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 30
·7431-7440
·
IF 4.157
Yang Shirui,Zhang Wenxiang,Liu Zheyi,Zhai Ziyang,Hou Xudong,Wang Ping,Ge Guangbo,Wang Fangjun
Human serum albumin (HSA) is one of the most important serum carrier proteins that deliver small-molecule drugs to their specific targets. Clarifying the molecular mechanism of the interaction between natural HSA and drugs in an aqueous solution has been a hot topic in pharmaceutical chemistry, clinical medicine, and biochemistry in recent years, but it is still challenging. In this paper, the details of molecular interactions of HSA with a variety of therapeutic drugs including ibuprofen, indomethacin, phenylbutazone, and warfarin are systematically investigated using a mass spectrometry (MS)-based lysine reactivity profiling (LRP) strategy. The results reaffirm that the major ligand binding sites (including Sites I and II) of HSA are located in subdomains IIA and IIIA, while several potential drug-binding areas at subdomain IIIB and α helix IIB-IIIA are newly characterized. The MS-LRP strategy may have important application prospects in pharmacodynamics, pharmacokinetics, and safety evaluation of small-molecule drugs. Graphical abstract
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Shuifang Mao,Bin Wang,Lin Yue,Wenshui Xia
In order to improve the anti-inflammatory activity of chitosan oligosaccharide (COS), chitosan oligosaccharide graft citronellol derivatives (COS-g-Cit1-3) were successfully synthesized via grafting citronellol (Cit) onto COS backbone. The degrees of substitution (DS) of COS-g-Cit1-3 were 0.165, 0.199 and 0.182, respectively. The structure of COS-g-Cit1-3 was confirmed by UV–vis, FT-IR, 1 H NMR and elemental analysis. The in vivo anti-inflammatory activity evaluation results displayed that COS-g-Cit1-3 drastically reduced the paw swelling, and the oedema inhibitions were 22.58 %, 29.03 % and 25.81 %, respectively. The results indicated that the anti-inflammatory effects of COS-g-Cit1-3 were significantly higher than COS and COS-g-Cit2 exhibited the highest anti-inflammatory ability. The results also presented that COS-g-Cit1-3 reduced the expression levels of TNF-α by promoting the secretion of IL-4 and IL-10. Moreover, western blot analysis data proved that COS-g-Cit1-3 inactivated the NF-κB signaling pathway via inhibiting the phosphorylation of p65, IKBα and IKKβ.
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BMC Complementary Medicine and Therapies
·
2021
·Vol. 21
·期 1
·1-11
·
IF 0
Lou Hai-xia,Fu Wen-cheng,Chen Jia-xiang,Li Tian-tian,Jiang Ying-ying,Liu Chun-hui,Zhang Wen
Background Alisol A 24-acetate (AA-24-a), one of the main active triterpenes isolated from the well-known medicinal plant Alisma orientale (Sam.) Juz., exhibits multiple biological activities including hypolipidemic activity. However, its effect on lipid metabolism in adipocytes remains unclear. The present study aimed to clarify the effect of AA-24-a on adipocyte lipolysis and to determine its potential mechanism of action using 3 T3-L1 cells. Methods We assayed the release of glycerol into culture medium of 3 T3-L1 cells under treatment with AA-24-a. Protein and mRNA expression and phosphorylation levels of the main lipases and kinases involved in lipolysis regulation were determined by quantitative polymerase chain reaction and western blotting. Specific inhibitors of protein kinase A (PKA; H89) and extracellular signal-regulated kinase (ERK; PD98059), which are key enzymes in relevant signaling pathways, were used to examine their roles in AA-24-a-stimulated lipolysis. Results AA-24-a significantly stimulated neutral lipolysis in fully differentiated adipocytes. To determine the underlying mechanism, we assessed the changes in mRNA and protein levels of key lipolysis-related genes in the presence or absence of H89 and PD98059. Both inhibitors reduced AA-24-a-induced lipolysis. Moreover, pretreatment with H89 attenuated AA-24-a-induced phosphorylation of hormone-sensitive lipase at Ser660, while pretreatment with PD98059 attenuated AA-24-a-induced downregulation of peroxisome proliferator-activated receptor-γ and perilipin A. Conclusions Our results indicate that AA-24-a promoted neutral lipolysis in 3 T3-L1 adipocytes by activating PKA-mediated phosphorylation of hormone-sensitive lipase and ERK- mediated downregulation of expression of perilipin A.
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Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 26
·5272-5283
·
IF 6.331
Min He,Bo Yang,Fangjun Huo,Li Xie,Mo Yang,Weidong Tian
The immune response elicited by the bone endoprosthesis is currently considered an important factor that affects its interfacial osteointegration. In this work, a metal-phenolic-based drug-loaded coating with universal adhesion properties and intelligent drug delivery feature was created to promote osteointegration by manipulating a beneficial osteoimmune microenvironment. A novel pro-drug with inflammation-responsive release function was firstly synthesized via the esterification reaction between tannic acid (TA) and indometacin (IND), and then the coating was developed by chelating it with Fe3+. In the normal biological environment, the coating was stable, while, in the inflammatory environment, the release of TA and IND motifs could be triggered by the overexpressed esterase. The released TA and IND displayed synergistic effects on macrophage polarization, leading to a downregulation expression of pro-inflammatory cytokines, and an upregulation expression of anti-inflammatory cytokines and osteogenic-related factors. When stimulated by a conditioned medium generated by macrophages seeded onto the coating, the osteogenic differentiation potential of BMSCs was significantly enhanced. Finally, the designed coating significantly promoted the osteointegration of the implant, demonstrated by the increase of the bone-implant contact by two times. Additionally, the coating was substrate-independent and can be formed within seconds without special equipment, thus, it showed great potential applications to endow advanced hard tissue implants with favorable osteoimmunomodulation.
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Frontiers in Pharmacology
·
2020
·
IF 4.225
Liu Tengwen,Guo Yuhong,Zhao Jingxia,He Shasha,Bai Yunjing,Wang Ning,Lin Yan,Liu Qingquan,Xu Xiaolong
The outbreak of coronavirus disease 2019 (COVID-19) has affected millions of people worldwide. Critically ill COVID-19 patients develop viral septic syndrome, including inflammatory damage, immune dysfunction, and coagulation disorder. In this study, we investigated ShenFuHuang formula (SFH), a traditional Chinese medicine, which has been widely used as complementary therapy for clinical treatment of COVID-19 in Wuhan, to understand its pharmacological properties. Results of systems pharmacology identified 49 active compounds of SFH and their 69 potential targets, including GSK3β, ESR1, PPARG, PTGS2, AKR1B10, and MAPK14. Network analysis illustrated that the targets of SFH may be involved in viral disease, bacterial infection/mycosis, and metabolic disease. Moreover, signaling pathway analysis showed that Toll-like receptors, MAPK, PPAR, VEGF, NOD-like receptor, and NF-kappa B signaling pathways are highly connected with the potential targets of SFH. We further employed multiple zebrafish models to confirm the pharmacological effects of SFH. Results showed that SFH treatment significantly inhibited the inflammatory damage by reducing the generation of neutrophils in Poly (I:C)-induced viral infection model. Moreover, SFH treatment could improve the phagocytosis of macrophages and enhance the expression of immune genes in an immune deficiency model. Furthermore, SFH treatment exhibited promising anti-thrombosis effect in a thrombus model. This study provided additional evidence of SFH formula for treating COVID-19 patients with septic syndrome using multiple-scale estimation.
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ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Kun Fang,Rong Wang,Hua Zhang,Linjie Zhou,Ting Xu,Ying Xiao,Yang Zhou,Guorong Gao,Jing Chen,Donglei Liu,Fanrong Ai,Jun Fu
Acute wounds subject to frequent deformations are difficult to be treated because the healing process was easily interfered by external mechanical forces. Traditional wound dressings have limited efficacy because of their poor mechanical properties and skin adhesiveness and difficulty in the delivery of therapeutic drugs effectively. As such, tough and skin-adhesive wound dressings with sustainable and stimuli-responsive drug release properties for treatment of those wounds are highly desirable. For this purpose, we have developed a mechano-responsive poly(sulfobetaine methacrylate) hydrogel which aims to control the delivery of antibiotic drug upon application of mechanical forces. Diacrylated Pluronic F127 micelles were used as a macro-cross-linker of the hydrogel and loaded with hydrophobic antimicrobial drugs. The micelle-cross-linked hydrogel has excellent mechanical properties, with the ultimate tensile strength and tensile strain of up to 112 kPa and 1420%, respectively, and compressive stress of up to 1.41 MPa. Adhesiveness of the hydrogel to the skin tissue was ∼6 kPa, and it did not decrease significantly after repetitive adhesion cycles. Protein adsorption on the hydrogel was significantly inhibited compared to that on commercial wound dressings. Because of the mechano-responsive deformation of micelles, the release of drug from the hydrogel could be precisely controlled by the extent and cycles of mechanical loading and unloading, endowing the hydrogel with superior antibacterial property against both Gram-positive and Gram-negative bacteria. In addition, drug penetration into the skin tissue was enhanced by mechanical stress applied to the hydrogel. The micelle-cross-linked zwitterionic hydrogel also showed good cell biocompatibility, negligible skin irritation, and healing capacity to acute skin wounds in mice. Such a tough mechano-responsive hydrogel holds great promise as wound dressings for acute wounds subjected to frequent movements.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-10
·
IF 5.833
Xu Xu,Feng Xue,Liu Zhen,Xue Shan,Zhang Lei
A low cost-effective and simple synthesis method was adopted to acquire three-dimensional flower-like structure Fe 3 O 4 /C that has large specific area, suitable pore structure and sufficient saturation magnetism. The obtained Fe 3 O 4 /C exhibits outstanding preconcentration ability and was applied to extracting non-steroidal anti-inflammatory drugs from complex environmental and biological samples. The parameters of magnetic solid-phase extraction were optimized by univariate and multivariate methods (Box-Behnken design). The high degree of linearity from 2.5 to 1000.0 ng mL −1 (R 2 ≥ 0.9976), the limits of detection from 0.25 to 0.5 ng mL - 1 (S/ N = 3), and the limits of quantitation from 1.0 to 2.0 ng mL - 1 (S/ N = 10) were yielded by adopting this novel method after the optimization. Moreover, the recoveries of non-steroidal anti-inflammatory drugs from 89.6 to 107.0% were acquired in spiked plasma, urine and lake samples. In addition, the adsorption of non-steroidal anti-inflammatory drugs on Fe 3 O 4 /C was explored by adsorption isotherms and kinetic studies. Furthermore, the adsorption mechanism for non-steroidal anti-inflammatory drugs by Fe 3 O 4 /C was proposed, which was hydrogen bonding and π-π interaction between non-steroidal anti-inflammatory drugs and Fe 3 O 4 /C.
Graphical abstract
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危险品化学品经营许可证（带存储）