MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jing Xiao,Dali Zhuo,Jiayuan Tang,Jihong Chen,Chao Tan,Shu Zhang,Shichao Li,Zhirong Zou
The analysis of cyclamate in food samples holds a significant implication for human health, necessitating the development of a selective, sensitive and user-friendly analytical method for analysis of cyclamate. Herein, a pressure meter-based analytical kit was established and applied to selective and sensitive detection of cyclamate. Cyclamate undergoes a selective reaction with NaNO 2 within a hermetically sealed reaction vessel. The reaction produces cyclohexene and N 2 , leading to a discernible pressure change that can be accurately measured using a portable gas pressure meter. Within the concentration range of 20–1000 mg/L, gas pressure values exhibit a proportional relationship with cyclamate concentrations, with a limit of detection of 5.6 mg/L. This analytical kit was further applied to assess cyclamate levels in various beverages and a standard quality control sample (BWQ8791-2016), with recoveries of 93–108 %. It is a simple, easy used, selective and sensitive analytical system for analysis of cyclamate in beverages.
研究领域:
食品安全
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY
·
2014
·Vol. 20
·期 7
·883-888
·
IF 1.735
Zhang Zhongzheng,Wang Hui,Chen Xinqing,Xie Rongyong,Gao Peng,Wei Wei,Sun Yuhan
The sorption isotherms of CO 2 in wet ordered mesoporous silica KIT-6 with different amounts of pre-adsorbed water were firstly collected experimentally using a volumetric method in the temperature range of 275–281 K. The isotherms show an inflection point indicating CO 2 hydrates form in the pore spaces which is proofed by the enthalpy change calculated at the inflection pressure, and the quantity of water content shows considerable effect on the sorption capacity of CO 2 . The highest enhancement of sorption capacity in the presence of water is observed in wet KIT-6 sample with water loadings of 2.48, which is about 12.80 mmol/g and 1.86 times than that on dry sample. However, the saturation capacity is still far less than that what can be stored merely in the form of hydrates due to the low ratio of water utilization because of the large pore and the polar surface of KIT-6.
研究领域:
材料科学
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环境工程
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吸附技术
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碳捕集与封存
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zeng Ronghua,Zhuo Dali,Dong Xiaoyu,Tang Jiayuan,Liu Yuxin,Zhang Shu,Luo Hong,Xu Fengzhou,Zou Zhirong
Sulfide ions (S 2− ) are a hazardous pollutant present in our daily lives; thus, the accurate detection of S 2− is critical for human health. However, conventional analytical methods have the disadvantage of high costs, complex operations, and the need for laboratory analysis. In this work, a miniaturized analytical kit was established for rapid analysis of S 2− in water samples and food additives. In the presence of platinum nanoparticles (NPs), H 2 O 2 was decomposed to O 2 catalyzed by Pt NPs, resulting in a substantial increase in pressure inside the sealed reaction bottle. In the presence of S 2- , S 2− forms a Pt–S bond with Pt NPs and induces aggregation, thereby reducing the catalytic activity of Pt NPs, resulting in a decrease in O 2 production and a corresponding decrease in gas pressure. Thus, the analysis of S 2− was achieved by the measurement of gas pressure. S 2− concentrations were inversely proportional to the gas pressures within a linear range of 0.2–5 μg/mL. A portable analytical kit was fabricated for rapid analysis of S 2− in various samples, achieving recoveries of 90–109%, confirming its suitability as a potential analytical device for on-site environmental analysis and rapid food inspection. Graphical
研究领域:
环境分析
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食品安全
·
分析化学
·
纳米材料
·
传感器技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2023
·
IF 8.7
Qiaofei Zhang,Wenlei Xie,Jiangbo Li,Lihong Guo
For the purpose of fabricating an efficient solid acid catalyst for biodiesel production from low-grade acidic oils, bimetallic silica mesoporous composites with different Zr/Al molar ratios were initially synthesized via a facile one-step hydrothermal method , and then sulfated with sulfuric acid at different concentrations to get a series of sulfated Zr x -Al y -KIT-6 catalysts (denoted as SZA-K, where x/y represents the molar ratio of Zr to Al). The sulfated bimetallic solid catalysts were fully characterized by using XRD , FT-IR, TEM, SEM, EDS, NH 3 -TPD, and nitrogen porosimetry measurements. Results showed that the remarkable amount of acidic sites in the catalysts was derived from the Zr and Al incorporation and further sulfation, and specifically, the Zr 4+ and Al 3+ could afford abundant weak acid sites while the inductive effect of SO 4 2− species greatly influences the acidic strength of this solid catalyst. In combination with the enhancement in mass transfer resulting from the inherent interconnected mesoporous structure, the SZA-K catalyst could facilitate the transesterification reaction to take place efficiently. The sulfated Zr 5 –Al 1 -KIT-6 catalyst (Zr/Al molar ratio of 5/1, treated using 0.75 mol/L sulfuric acid for 12 h) exhibited enhanced acid strength with larger number of acidic sites (7.81 mmol/g), resulting in a high catalytic performance to the oil transesterification reaction. An oil conversion of 96.3% was attained for the transesterification reaction performed at 120 °C for 7 h by using a methanol/oil molar ratio of 20:1 and a catalyst dosage of 6 wt%. Further, this solid catalyst exhibited better acid and water-resistance capacity with above 80% of oil conversion even with 10% of free fatty acid (FFA) and 2% of moisture present in the low-grade acidic oils. Meanwhile, the good reusability was shown for this catalyst with 80.1% conversion even after reusing for three cycles. The good stability of the solid catalyst was resulted from the strong interactions between the acidic species and the bimetallic composite support.
研究领域:
化学工程
·
催化材料
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2017
·Vol. 312
·期 3
·599-607
·
IF 1.282
Liu Guiqing,Wang Wei,Lin Jianguo,Li Ke,Lv Gaochao,Zhao Xueyu,Wang Shanshan,Luo Shineng,Qiu Ling
16α- 18 F-fluoroestradiol ( 18 F-FES) has been developed as a promising positron emission tomography (PET) imaging agent for targeting estrogen receptor positive (ER + ) breast cancer in the clinical trial. However, the radiosynthesis of 18 F-FES often requires two steps and tough experimental conditions. Therefore, a new estradiol derivative ( 18 F-AmBF 3 -ES) was prepared by an efficient one-step 18 F-radiolabeling method. The tracer was obtained in high yield (~65%) and excellent radiochemical purity (>98%) within 30 min. The uptake rate of 18 F-AmBF 3 -ES in ER + cells was about 3.5% at 30 min. The results suggested that the tracer may be a potential PET imaging agent for ER + breast cancer.
研究领域:
医学影像学
·
肿瘤学
·
核化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Min Zhang,Xiaoli Zhang,Baofeng Qu,Jinhua Zhan
In this work, a portable kit was developed for convenient high-throughput trace analysis of polycyclic aromatic hydrocarbons (PAHs) using surface enhanced Raman scattering (SERS) after dispersive liquid-liquid microextraction (DLLME) process. This portable kit contains three sealed reagent tubes (labeled as T1, T2 and T3), a self-made well plate, and a portable Raman spectrometer. The reagent tube T1 contains a mixture of disperser solvent and extraction solvent, which involved a 2 min sample pretreatment of DLLME process. The quick injection of solvents in tube T1 into the sample containing PAHs formed a cloudy solution immediately, which consists of fine droplets of extraction solvent dispersed entirely into aqueous phase. The enrichment factor was found to be 29.6. T2 and T3 contain methanol and 1-propanethiol-modified silver nanoparticles (PTH-Ag NPs), respectively. The liquid in the tube T3 was used to enhance the Raman signal of analytes on the self-made high-throughput micro reactor. A linear relationship between the concentration of pyrene and the relative Raman peak intensity was obtained (R 2 = 0.993). The detection limit was 0.50 μg L −1 for pyrene. RSD of the high-throughput analysis of 12 samples was calculated as 4.8%. The ability of DLLME-SERS technique in the extraction of PAH isomers from water samples were investigated. The performance of DLLME-SERS in the recovery of pyrene from lake, spring and drinking water was also studied.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Siyang Deng,Junmei Liu,Dong Han,Xinting Yang,Huan Liu,Chunhui Zhang,Christophe Blecker
A novel ratiometric fluorescent nanoprobe (Rh6G@UIO-66-NH 2 ) was fabricated for efficient nitrite (NO 2 – ) detection in the present study. When NO 2 – was introduced, it interacted with the amino groups on the surface of Rh6G@UIO-66-NH 2 , forming diazonium salts that led to the quenching of blue fluorescence. With this strategy, a good linear relationship between NO 2 – concentration and the fluorescent intensity ratio of the nanoprobe in the range of 1–100 μM was established, with a detection limit of 0.021 μM. This dual-readout nanosensor was applied to analyze the concentration of NO 2 – in real meat samples, achieving satisfactory recovery rates of 94.72–104.52%, highlighting the practical potential of this method. Furthermore, a portable Gel/Rh6G@UIO-66-NH 2 hydrogel test kit was constructed for on-spot dual-mode detection of NO 2 – . This kit allows for convenient colorimetric analysis and fluorometric detection when used in conjunction with a smartphone. All the photos taken with the portable kit was converted into digital information using ImageJ software. It provides colorimetric and fluorescent visual detection of NO 2 – over a range of 0.1–1.5 mM, achieving a direct quantitative tool for NO 2 – identification. This methodology presents a promising strategy for NO 2 – detection and expands the application prospects for on-spot monitoring of food safety assessment.
研究领域:
分析化学
·
食品科学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC CANCER
·
2019
·Vol. 19
·期 1
·1-9
·
IF 2.933
Xiao Hui,He Mudan,Xie Guogang,Liu Yanan,Zhao Yuxia,Ye Xiong,Li Xingjing,Zhang Min
Background Cancer cells release exosomes and can be taken up by mast cells (MCs), but the potential functional effects of MCs on tumor metastasis remain unknown.MethodExosomes were isolated from the lung adenocarcinoma cell line A549, and the uptake of PKH26-labeled exosomes by bone marrow MCs was examined via flow cytometry and fluorescence microscopy. Cytokines and tryptase in MC supernatant were analyzed using an ELISA kit, and the presence of tryptase was evaluated by Western blotting. Cell proliferation and migration were determined through CCK-8 and transwell assays. Proteins in the tryptase-JAK-STAT signaling pathway were detected by Western blotting.Results In this study, we show that exosomes from A549 cells can be taken up by MCs. Moreover, A549 exosomes contain stem cell factor (SCF) to MCs and subsequently induce the activation of MCs through SCF-KIT signal transduction, which leads to MC degranulation and the release of tryptase. Tryptase accelerates the proliferation and migration of human umbilical vein endothelial cells (HUVECs) through the JAK-STAT signaling pathway.Conclusion sOur results reveal a mechanism for metastasis in which exosomes can transfer SCF to and activate MCs, which can affect the release of tryptase and the angiogenesis of HUVECs.
研究领域:
肿瘤学
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细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Qi Wenbo,Xu Lijian,Zhao Shuangdan,Zheng Jiahua,Tian Yanpeng,Qi Xuejun,Huang Xianghua,Zhang Jingkun
Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1α releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing.
研究领域:
组织工程
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再生医学
·
生物材料
·
生殖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2016
·
IF 3.146
Yang Liu,Yachen Xu,Zhenhua Wang,Dezhong Wen,Wentian Zhang,Sebastian Schmull,Haiyan Li,Yao Chen,Song Xue
Electrospun nanofibrous sheets get increasing attention in myocardial infarction (MI) treatment due to their good cytocompatibility to deliver transplanted stem cells to infarcted areas and due to mechanical characteristics to support damaged tissue. Cardiac extracellular matrix is essential for implanted cells since it provides the cardiac microenvironment. In this study, we hypothesized high concentrations of cardiac nature protein (NP), namely elastin and collagen, in hybrid polycaprolactone (PCL) electrospun nanofibrous sheets could be effective as cardiac-mimicking patch. Optimal ratio of elastin and collagen with PCL in electrospun sheets (80% NP/PCL) was selected based on cytocompatibility and mechanical characteristics. Bone-marrow (BM) c-kit + cells anchoring onto NP/PCL sheets exhibited increased proliferative capacity compared with those seeded on PCL in vitro. Moreover, we examined the improvement of cardiac function in MI mice by cell-seeded cardiac patch. Green Fluorescent Protein (GFP)-labeled BM c-kit + cells were loaded on 80% NP/PCL sheets which was transplanted into MI mice. Both 80% NP/PCL and c-kit + -seeded 80% NP/PCL effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. C-kit + -seeded 80% NP/PCL was even superior to the 80% NP/PCL alone and both superior to PCL. GFP + cells were identified both in the sheets and local infarcted area where transplanted cells underwent cardiac differentiation after 4 weeks. To the best of our knowledge, this is the first report that sheets with high concentrations of nature proteins loaded with BM c-kit + cells might be a novel promising candidate for tissue-engineered cardiac patch to improve cardiac repair after MI.
研究领域:
生物医学工程
·
心脏病学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
World Journal of Gastrointestinal Surgery
·
2023
·
IF 2
Fan Ding,Run Guo,Fang Chen,Li-Ping Liu,Zheng-Yu Cui,Yi-Xing Wang,Gang Zhao,Hai Hu
BACKGROUND Impaired interstitial cells of Cajal (ICCs) are central to the pathophysiology of acute cholecystitis (AC). Common bile duct ligation is a common model of AC, producing acute inflammatory changes and decrease in gallbladder contractility. AIM To investigate the origin of slow wave (SW) in the gallbladder and the effect of ICCs on gallbladder contractions during the process of AC. METHODS Methylene blue (MB) with light was used to establish selective impaired ICCs gallbladder tissue. Gallbladder motility was assessed using the frequency of SW and gallbladder muscle contractility in vitro in normal control (NC), AC12h, AC24h, and AC48h groups of guinea pigs. Hematoxylin and eosin and Masson-stained gallbladder tissues were scored for inflammatory changes. ICCs pathological changes alterations were estimated using immunohistochemistry and transmission electron microscopy. The alterations of c-Kit, α-SMA, cholecystokinin A receptor (CCKAR), and connexin 43 (CX43) were assessed using Western blot. RESULTS Impaired ICCs muscle strips resulted in the decrease in gallbladder SW frequency and contractility. The frequency of SW and gallbladder contractility were significantly lower in the AC12h group. Compared with the NC group, the density and ultrastructure of ICCs were remarkably impaired in the AC groups, especially in the AC12h group. The protein expression levels of c-Kit were significantly decreased in the AC12h group, while CCKAR and CX43 protein expression levels were significantly decreased in the AC48h group. CONCLUSION Loss ICCs could lead to a decrease in gallbladder SW frequency and contractility. The density and ultrastructure of ICCs were clearly impaired in the early stage of AC, while CCKAR and CX43 were significantly reduced at end stage.
研究领域:
胃肠病学
·
生理学
·
病理生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yueyuan Zhang,Xiaolong Chen,Zhuye Shang,Zhiqiang Zhang,Run Zhang,Qingtao Meng
Copper ions (Cu 2+ ), as essential trace elements, play a critical role in maintaining human physiological functions. However, disordered copper homeostasis, particularly excessive Cu 2+ accumulation, has been implicated in the pathogenesis of various disorders. Moreover, the rapid advancement of modern industrial and agricultural practices has led to widespread Cu 2+ contamination in the environment and food. Consequently, the development of a multifunctional portable test kit is significant for quantifying Cu 2+ levels. In this work, a near-infrared (NIR) fluorescent probe ( ZYC ) was successfully developed for the detection of Cu 2+ in several real samples. The probe ZYC exhibited outstanding performances, including NIR emission ( λ em = 660 nm), high selectivity and sensitivity (LOD = 11.7 nM), rapid response (30 s) and low cytotoxicity. The qualitative detection of elevated Cu 2+ levels in soil samples was successfully achieved using ZYC . Remarkably, a portable test kit was designed, allowing it to be used for the rapid and on-site detection of excessive Cu 2+ in herbs and vegetables. Furthermore, the fluorescence imaging of exogenous Cu 2+ in live MCF-7 cells, zebrafish and nude mice was successfully achieved utilizing probe ZYC . Consequently, probe ZYC provides a new method for elucidating the pathogenic mechanisms of Cu 2+ -associated diseases, while the development of a rapid and on-site Cu 2+ portable test kit provides a simple and convenient tool for monitoring food safety and environmental pollution.
研究领域:
环境科学
·
食品安全
·
分析化学
·
生物成像
·
传感器与生物传感器
·
毒理学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）