Polymers
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2015
·Vol. 7
·期 3
·468-483
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IF 3.681
Dongjian Shi,Jinting Hua,Li Zhang,Mingqing Chen
Novel bio-based aliphatic copolyesters, poly(lactic acid-co-10-hydroxy decanoate) (P(LA-co-HDA), PLH), were successfully synthesized from lactic acid (LA) and 10-hydroxycapric acid (HDA) by a thermal polycondensation process, in the presence ofp-toluenesulfonic acid (p-TSA) and SnCl2·2H2O as co-catalyst. The copolymer structure was characterized by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR). The weight average molecular weights (Mw) of PLH, from gel permeation chromatography (GPC) measurements, were controlled from 18,500 to 37,900 by changing the molar ratios of LA and HDA. Thermogravimetric analysis (TGA) results showed that PLH had excellent thermal stability, and the decomposition temperature at the maximum rate was above 280 °C. The glass transition temperature (Tg) and melting temperature (Tm) of PLH decreased continuously with increasing the HDA composition by differential scanning calorimetry (DSC) measurements. PLH showed high ductility, and the breaking elongation increased significantly by the increment of the HDA composition. Moreover, the PLH copolymer could degrade in buffer solution. The cell adhesion results showed that PLH had good biocompatibility with NIH/3T3 cells. The bio-based PLH copolymers have potential applications as thermoplastics, elastomers or impact modifiers in the biomedical, industrial and agricultural fields.Keywords:bio-based copolymer;poly(lactic acid);poly(10-hydroxycapric acid);high performance;mechanical properties
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Polymer Chemistry
·
2016
·Vol. 7
·期 14
·2553-2564
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IF 5.687
Yating Jia,Weizhong Wang,Xiaojun Zhou,Wei Nie,Liang Chen,Chuanglong He
Poly(glycerol sebacate) (PGS) has been explored extensively for tissue engineering applications due to its elastomeric mechanical properties and biocompatibility, but its relatively low mechanical strength and poor hydrophilicity restrict its practical applications in tissue engineering. In this study, we developed new biodegradable elastomeric copolyesters poly(glycerol-co-sebacic acid-co-L-lactic acid-co-polyethylene glycol) (PGSLP) by simultaneously introducing L-lactic acid (LLA) and polyethylene glycol (PEG) into the PGS network. The synthesized copolyesters were well-characterized to evaluate their physicochemical properties, mechanical strength and in vitro degradation properties, and their biocompatibility was also evaluated by a combination of in vivo and in vitro experiments. It is found that the PGSLP exhibited typical elastic properties, and possessed better hydrophilicity, water uptake capacity as well as improved degradation behaviors compared to PGS, which were beneficial for tissue engineering applications. More importantly, the physicochemical properties of PGSLP could be tuned in a wide range by changing the content of LLA and PEG, and their mechanical properties were visibly influenced by the curing conditions such as curing time. Furthermore, the L929 mouse fibroblast cells cultured on the PGSLP exhibited high viability, good spreading and proliferation, and PGSLP implants did not induce obvious inflammatory reaction but promoted neovascularization after subcutaneous implantation in Sprague-Dawley rats for 5 weeks. Hence, the developed PGSLP copolyesters may offer a much better choice of scaffolds than PGS for practical tissue engineering applications.
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Journal of Applied Biomaterials & Functional Materials
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2016
·
IF 0.934
Junyan Yao,Shijie Zhang,Wudan Li,Yujie Li
BackgroundThe amorphous poly(lactic acid-co-lysine) (PLL) with hydrophilic reactive groups was synthesized by the condensing copolymerization and the blending miscibility of poly(L-lactic acid) (PLLA) and PLL was investigated.MethodsThe miscibility of PLLA and PLL was evaluated by analyzing the thermal properties, crystallization behaviors, crystal morphologies and mechanical properties of the PLLA/PLL blends.ResultsThe results indicated that amorphous PLL changed the crystallization behaviors and thermal properties of PLLA, decreasing the crystallinity of PLLA in the blends. The amorphous PLL molecule chains in PLLA/PLL blends were able to enter into the lamellars of PLLA spherulites and affect the crystallization behaviors of PLLA, resulting in imperfect, spherulite structures of PLLA. The formation of hydrogen bonds between PLLA molecular chains and PLL molecular chains enabled partial compatibility in this blend system.ConclusionsThe existence of PLL improved the hydrophility of PLLA/PLL blends, led to a higher content of PLL in the PLLA/PLL blend system, and better hydrophilic properties of the blend system. The PLL was partially miscible with the PLLA, and the PLLA/PLL blend with 10 wt% PLL had improved tensile properties.
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RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2024
·Vol. 38
·期 15
·e9769
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IF 1.8
Liwei Xu,Andong Zha,Xia Xiong,Dehui Sun
Rationale Succinic acid and lactic acid have been associated with diarrhea in weaned piglets. The level of succinic acid and lactic acid in serum, meat, and intestinal contents is important to elucidate the mechanism of diarrhea in weaned piglets. Methods A facile method was developed for the quantification of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC/MS/MS). The serum samples underwent protein precipitation with methanol. The meat and intestinal contents were freeze-dried and homogenized using a tissue grinding apparatus. Methanol–water mixture (80:20, v/v) was used for homogenizing the meat, while water was used for homogenizing the intestinal contents. An additional step of protein precipitation with acetonitrile was required for the intestinal contents. The resulting solution was diluted with water before being analyzed by UHPLC/MS/MS. Separation of succinic acid and lactic acid could be achieved within 3 min using a Kinetic XB-C 18 column. Results The coefficients of variation for peak areas of succinic acid and lactic acid were less than 5.0%. The established method demonstrated good linearity as indicated by correlation coefficients exceeding 0.996. Additionally, satisfactory recoveries ranging from 88.58% to 108.8% were obtained. The detection limits ( R S/N = 3) for succinic acid and lactic acid were determined to be 0.75 ng/mL and 0.02 μg/mL, respectively. Conclusion This method exhibited high sensitivity, simplicity in operation, and small sample weight, making it suitable for quantitative determination of succinic acid and lactic acid in pigs' serum, intestinal contents, and meat. The method developed will provide valuable technical support in studying the metabolic mechanisms of succinic acid and lactic acid in pigs.
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Advanced Science
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2024
·
IF 14.3
Yuanting Xie,Jinhua He,Wenqing He,Tayyaba Iftikhar,Chuangjie Zhang,Lei Su,Xueji Zhang
Advancing the development of point-of-care testing (POCT) sensors that utilize interstitial fluid (ISF) presents considerable obstacles in terms of rapid sampling and analysis. Herein, an innovative strategy is introduced that involves the use of a 3D-printed, hollow microneedle array patch (MAP), in tandem with a vacuum tube (VT) connected through a hose, to improve ISF extraction efficiency and facilitate expedited analysis. The employment of negative pressure by the VT allows the MAP device to effectively gather ≈18 µL of ISF from the dermis of a live rabbit ear within a concise period of 5 min. This methodology enables the immediate and minimally invasive measurement of glucose levels within the body, employing personal healthcare meters for quantification. The fusion of the VT and MAP technologies provides for their effortless integration into a comprehensive and mobile system for ISF analysis, accomplished by preloading the hose with custom sensing papers designed to detect specific analytes. Moreover, the design and functionality of this integrated VT-MAP system are intuitively user-friendly, eliminating the requirement for specialized medical expertise. This feature enhances its potential to make a significant impact on the field of decentralized personal healthcare.
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SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Ruizhi Yu,Zejun Deng,P.E. Sharel,Lingcong Meng,Run Wang,Yijia Wang,Haichao Li,Xiang Wang,Kechao Zhou,Li Ma,Quiping Wei
Boron-doped diamond (BDD) microsensors feature exceptional advantages such as long-term stability, excellent antifouling properties, and ultra-low background currents, considered the ideal biosensors for non-enzymatic quantification of analytes. Herein, we reported, for the first time, the use of dual BDD electrode metallic BDD films on BDD microparticles) for enzyme-free detection of uric acid in complicated sweat environments. The BDDME with a dimension of 450 μm exhibited a fast electron transfer kinetic constant of 1.68 × 10 −2 cm s −1 towards K 3 [Fe (CN) 6 ] and K 4 [Fe (CN) 6 ] and a large electrochemically active surface area of 0.13 mm 2 , larger than the real geometric surface area. The voltammetric response currents of uric acid in sweat environments followed a good linear relation with increasing concentrations in the range of 5 μM - 500 μM, along with a detection sensitivity of 16.02 μA μM −1 cm −2 and a detection limit of 0.77 μM. In addition, the BDDME is capable of achieving highly specific quantification of uric acid in the presence of diverse interference agents such as glucose, urea, and tyrosine. In addition, the BDDME showed long-term stability with 4.6 % fluctuations in the response current during repetitive tests for 360 days and could be fully activated by rinsing with water despite the possible adsorption fouling of urine and bovine serum albumin during tests. This work demonstrated the feasibility of long-term detection of sweat uric acid using a non-enzymatic way and provides a solution for the long-term management of gout and other diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Tao Sun,Ying Li,Yangyang Wang,Qing Yang,Jiahui Du,Jian Hu,Sarula,Tungalag Dong,Xueyan Yun
Biodegradable poly(L-lactic acid) (PLLA) has seldom used for dairy packaging due to medium permeability and brittleness. Novel PLLA copolymers, poly (L-lactic acid- co -butylene itaconate-co-glycolic acid) (PLBIGA), were developed by integrating glycolic acid (GA) and poly(butylene itaconate) (PBI) into PLLA's structure using low molecular weight PLLA as a key initiator. Then, packaging materials with better barrier and mechanical properties were obtained by blended PLBIGA with PLLA. Both PLLA/PLBIGA films and polyethylene nylon composite film (PE/NY) were used for stirred yogurt packaging and storage at 4 °C for 25 days. Results revealed that yogurt packed by PLLA/PLBIGA films maintained stabler water-holding capacity, color, and viscosity over the storage period. Moreover, the integrity of the gel structure and the total viable count of lactic acid bacteria in yogurt packaged in PLLA/40-PLBIGA8 were also found to be superior to those in PE/NY packages, highlighting its eco-friendly advantages in dairy packaging.
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FOOD CHEMISTRY
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2024
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IF 8.5
Ziwen Wang,Dongdong Wang,Jiaxuan Fang,Zixin Song,Jiman Geng,Jianfei Zhao,Yifan Fang,Changtao Wang,Meng Li
In this study, an ultrasonic-assisted natural deep eutectic solvent (NaDES) was used to extract flavonoids from Perilla frutescens (L.) Britt. leaves. Of 10 tested NaDESs, that comprising D-(+)-glucose and glycerol exhibited the best total flavonoid extraction rate. Response surface methodology (RSM) was used for extraction modeling and optimization, and the total flavonoid content reached 87.48 ± 1.61 mg RE/g DW, which was a significant increase of 5.36% compared with that of 80% ethanol extraction. Morphological changes in P. frutescens leaves before and after extraction were analyzed by scanning electron microscopy (SEM), and the mechanism of NaDES formation was studied by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, 10 flavonoids were identified by UPLC-Q-TOF-MS. In addition, the NaDES extract had better biological activity according to several antioxidant, cyclooxygenase-2 (COX-2) and hyaluronidase (Hyal) inhibition experiments. Moreover, the stability test revealed that the total flavonoid loss rate of the NaDES extract after four weeks was 37.75% lower than that of the ethanol extract. These results indicate that the NaDES can effectively extract flavonoids from P. frutescens leaves and provide a reference for further applications in the food, medicine, health product and cosmetic industries.
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MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Xinwen Jia,Mengyun Lu,Wenjing Zhang,Wuduo Zhao,Ajuan Yu,Shusheng Zhang
Chiral carbon quantum dots (CQDs) with –SH, –NH 2 functional groups were grafted onto a metal–organic cage (Zn-MOC) with unsaturated metal sites through coordination bond, and then a novel bichiral composite Zn-MOC@CQDs was constructed for the chiral discrimination of LA enantiomers. Zn-MOC@CQDs exhibited a high fluorescence detection sensitivity for L -LA instead of D -LA, and the enantioselectivity coefficient for LA enantiomers was 2.34. Zn-MOC@CQDs exhibited more excellent enantioselectivity than the single components of CQDs and Zn-MOC, and the key reason may be the chiral synergistic effect between the different chiral building blocks.
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TALANTA
·
2024
·
IF 5.6
Yongjing Liu,Dongting Huang,Chenxi Zhuo,Min Guo,Lirong Chen,Xiaofen Chen,Hua Li,Wei Xu
A series of supramolecular deep eutectic solvents (SURPADESs) consisting of β -cyclodextrin ( β -CDs) ( β -CD, HP- β -CD, M- β -CD) and l -lactic acid were synthesized and employed as chiral selector to investigate their chiral selectivity in capillary electrophoresis. Key elements influencing the separation efficiency, such as the ratio of HBA/HBD of SUPRADESs, the concentration of SUPRADESs, and buffer pH were thoroughly examined and deliberated upon. Under the optimum enantioseparation conditions, three SUPRADESs systems showed markedly enhanced chiral separation of model chiral drugs, in comparison with single CDs and dual chiral selectors composed by adding its two constituents CDs and l -lactic acid separately. Additionally, the Benesi-Hildebrand plot was employed to determine the binding constant for the inclusion complexes, and molecular modeling was used to explore the mechanisms behind the increased chiral separations in SUPRADESs. Results demonstrated that SUPRADESs exhibit significantly improved chiral selectivity efficiency by enhancing the host-guest encapsulation of the constituent β -CDs, which is attributed to the increased hydrogen bonding and hydrophobic interactions between chiral analytes and β -CDs and HBD of SUPRADESs. This work provides a possibility for SUPRADESs to be used as chiral selection in CE.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Qiannan Zheng,Dengfeng Wang,Xiujin Gong,Lin Liu,Han Wu,Zihan Li,Haojie Hong,Juming Yao
The development of high-performance biodegradable polylactic acid (PLA) materials integrating high strength, malleability and toughness is desired but an ongoing challenge. In this work, a novel full-biobased block copolymer was designed and synthesized by grafting L (+)-lactide (L-LA) and ε -caprolactone ( ε -CL) onto lignin via ring-opening polymerization. The obtained lignin-PLA-PCL block copolymer was composed of rigid lignin and poly (LA-CL) rubber segment, could self-assemble into uniform nano-micelles with average diameters of 80–100 nm regulated by simply altering copolymer content. The incorporation of lignin-PLA-PCL copolymers into PLA matrix induced the formation of many cavities, promoted free volume between PLA matrix and copolymer to accelerate chain mobility, achieving excellent ductility and stretchability with maximum stretching deformation of 64.8 %. The resultant PLA composites with the copolymer content as low as 5 wt% displayed simultaneously improved strength (41.84 MPa) and toughness (8.1 MJ/m 3 ), 6.7 % and 1520 % increment than those of neat PLA, respectively. The reinforcing and toughening mechanisms were explored and verified that the combination of cavity growth and fibrillation, followed by extensive shear yielding of matrix, causing substantial plastic deformation. This study extended the design strategy and the foundation for simultaneous reinforcing and toughening PLA plastics using lignin-derived rubbery micelles.
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ACS Nano
·
2025
·
IF 16
Guoqing Fan,Yuheng Lu,Yubin Li,Jian Zhang,Yuanbin Wang,Pingyin Lee,Canquan Zhou,Rongkang Huang,Binghua Ma,Yuan Yuan
Regeneration of the injured endometrium, particularly the functional layer, is crucial for the prevention of uterine infertility. At present, clinical treatment using sodium hyaluronate hydrogel injection is limited by its relatively low fluidity, short-term retention, and insufficient bioactive ingredients, so it is necessary to develop an advanced healing-promoting hydrogel. The modulation of the microenvironment by Lactobacillus presents a bioactive component that can facilitate the regeneration of the functional layer. Our study introduces a multifunctional Lactobacillus-loaded poly(N-isopropylacrylamide)-grafted bacterial cellulose (BC-g-PN@L) hydrogel designed with superior injectability and in situ stability. At 25 °C (room temperature), a uniform distribution is achieved with a low injection pressure of only 7.90 kPa. At 37 °C (body temperature), the BC-g-PN@L hydrogel forms a robust three-dimensional nanonetwork, providing space and substance exchange channels for Lactobacillus to maintain its viability and bioactivity. Enhanced by the hydrophobic isopropyl groups in poly(N-isopropylacrylamide) side chains and the rigid bacterial cellulose substrates, the BC-g-PN@L hydrogel exhibits prolonged retention properties in the uterine cavity, persisting for over 21 days. These attributes endow the BC-g-PN@L hydrogel with versatile pro-healing capacity and microenvironment modulation in a rat model of endometrial injury. Our BC-g-PN@L hydrogel promotes the development of advanced injectable hydrogels to facilitate both histological and functional repair of the injured endometrium.
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