LANGMUIR
·
2024
·
IF 3.7
Jingyao Mao,Yuteng Zhang,Shensong Zhang,Bo Song
The fluorescent probe method has attracted significant research attention due to its high sensitivity and reproducibility in detecting bovine serum albumin (BSA). In this study, we constructed a fluorescent probe for BSA detection by assembling an amphiphilic organic fluorescent molecule, termed 2-(2’-hydroxyphenyl) benzothiazole (HBT-11), with BSA. In an aqueous solution, HBT-11 exhibited a weak fluorescence emission at 501 nm. However, the addition of BSA substantially enhanced the fluorescence emission at 501 nm, indicating that the assembly was driven by electrostatic interactions between HBT-11 and BSA. HBT-11, serving as a fluorescent probe for BSA detection, demonstrated a limit of detection (LOD) as low as 3.92 nmol L–1, excellent photostability, high selectivity, and robust anti-interference capability. Notably, we successfully applied HBT-11 for detecting BSA in fetal bovine serum and selectively imaging BSA in HeLa cells.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Cong Fan,Nike He,Jifeng Yuan
The rapid and accurate detection of fungal pathogens is of utmost importance in the fields of healthcare, food safety, and environmental monitoring. In this study, we implemented a cascaded amplifying circuit in Saccharomyces cerevisiae to improve the G protein-coupled receptor (GPCR) mediated fungal detection . The GPCR signaling pathway was coupled with the galactose-regulated (GAL) system and a positive feedback loop was implemented to enhance the performance of yeast biosensor. We systematically compared four generations of biosensors for detecting the mating pheromone of Candida albicans , and the best biosensor exhibited the limit of detection (LOD) as low as 0.25 pM and the limit of quantification (LOQ) of 1 pM after 2 h incubation. Subsequently, we developed a betaxanthin-based colorimetric module for the easy visualization of signal outputs, and the resulting biosensors can give reliable naked-eye readouts. In summary, we demonstrated that cascaded amplifying circuits could substantially improve the engineered yeast biosensors with a better sensitivity and signal output magnitude, which will pave the way for their real-world applications in public health.
研究领域:
生物技术
·
微生物学
·
生物医学工程
·
环境科学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zhiwei Qie,Qiqi Liu,Wenliang Yan,Zichen Gao,Wu Meng,Rui Xiao,Shengqi Wang
A universal and ultrasensitive immunochromatographic assay (ICA) was established using antigen as a bifunctional element and antialbumin antibody in a test line. Preincubation was introduced for competitive recognition. After optimization, the linear detection of aflatoxin M1 (AFM1) with quantum dot bead (QB)-based ICA (QB-ICA) sensor ranged from 10 to 52 pg mL–1, with a 50% inhibitory concentration (IC50) of 23 pg mL–1, which was nearly 49.6-fold lower than those of ICA on a traditional structure with traditional pretreatment (IC50 = 1.10 ng mL–1) and 10-fold lower than those of ICA on a traditional structure with acid aid pretreatment (IC50 = 0.25 ng mL–1). The limit of detection (LOD) for AFM1 was 16 pg mL–1 in milk, which was approximately 16.3-fold times higher than those of ICA on a traditional structure with traditional pretreatment and 6.3-fold higher than those of ICA on a traditional structure with acid aid pretreatment. The LOD improved by 20-fold by using the proposed structure compared to that of conventional enzyme-linked immunosorbent assay (ELISA) for AFM1-spiked milk samples (IC10 = 0.12 ng mL–1). The performance and practicability of the established QB-ICA sensor were validated with a commercial ELISA kit. To evaluate universality, we successfully detected chloramphenicol, with IC50 of 0.42 ng mL–1. Given its high sensitivity and universality, the proposed QB-ICA can be used as an alternative for rapid, sensitive, and universal quantitative detection of all small-molecule analytes.
研究领域:
分析化学
·
食品安全
·
生物技术
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2014
·
IF 3.163
Xiude Hua,Xiaofeng Liu,Wei Yin,Yazhong Xia,Qiujun Zhou,Yiwen Lu,Wei Li,Haiyan Shi,Fengquan Liu,Minghua Wang
A sensitive monoclonal antibody-based enzyme monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was developed to detect bifenthrin in a chemical soil barrier for termite control. LBc ((2-methyl[1,1-biphenyl]-3-methoxy) carbonyl propionic acid) was conjugated to bovine serum albumin (BSA) for producing monoclonal antibody. After optimization, the IC 50 and limit of detection (LOD, IC 10 ) were 0.05 mg L − 1 and 0.004 mg L − 1 , respectively. The sensitivity was improved 40-fold compared to polyclonal antibody-based ELISA reported earlier. No cross-reactivity was measured for the other analogues such as cyhalothrin, cyhalothric acid, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, ethofenprox and tetramethrin except 2-methyl-3-biphenylmethyanol. Spiked recoveries were between 83.5% and 104.7% for the detection of bifenthrin in loess, red soil and black soil. All the relative standard deviations (RSDs) were less than or equal to 15.0%. Moreover, the ELISA for authentic samples showed reliability and high correlation with gas chromatography. The developed ELISA is an alternative tool for simple, sensitive and accurate monitoring of bifenthrin in chemical soil barriers.
研究领域:
分析化学
·
环境科学
·
害虫控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 10
·1877-1887
·
IF 4.3
Wang Yinghui,Yu Ling,Zhang Hui,Zhu Runzhi,Meng Zhe
An accurate and sensitive competitive enzyme-linked immunosorbent assay (ELISA) based on persistent luminescence nanoparticles Zn 2 GeO 4 :Mn 2+ , Eu 3+ (ZGME) was developed for detecting ochratoxin A (OTA), a powerfully toxic mycotoxin usually found in grains. As a signal output element of autofluorescence-free biosensors, ZGME can be integrated into ELISA with glucose oxidase (GOx)-binding OTA molecules due to its excellent pH-responsive persistent luminescence. In the absence of OTA, the OTA-GOx conjugate was captured by the anti-OTA monoclonal antibody (anti-OTA mAb) pre-coated on the 96-well plate. The results indicate a decrease in the pH value of the solution, which triggered the quenching of ZGME luminescence due to GOx-dependent gluconic acid production. The presence of OTA inhibited the binding of OTA-GOx on the plate, thus decreasing the production of gluconic acid and increasing the persistent luminous intensity of ZGME. Under the optimized concentrations of anti-OTA mAb and OTA-GOx, quantitative determination of OTA was achieved by plotting the increase or decrease in persistent luminescence intensity of ZGME at 535 nm. In this study, the linear range was from 0.1 μg L −1 to 63 μg L −1 , and the limit of detection (LOD) was as low as 0.045 μg L −1 . In five food samples (corn grit, brown rice, soybean, rice, and wheat), the results exhibited good stability and repeatability, with a recovery range from 81.3% to 94.4% and a relative standard deviation (RSD) of less than 4.2%. Hence, the established method provides a sensitive, accurate, and autofluorescence-free approach for the determination of OTA in different grain samples.
研究领域:
分析化学
·
毒理学
·
食品科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2023
·Vol. 31
·期 16
·25662-25679
·
IF 3.8
Qin Tan,Shengnan Wu,Shengnan Wu,Shengnan Wu,Zhenchao Liu,Xiaolu Chen,Sailing He,Sailing He,Sailing He
Disturbance of surrounding temperature inevitably affects the accuracy of fiber biosensors. To that end, we propose a compact label-free optofluidic sensor based on a polished hollow core Bragg fiber (HCBF) that can simultaneously measure the cortisol concentration and surrounding temperature in real-time. The sensor is comprised of fusion splicing single mode fiber (SMF), multimode fiber (MMF) and HCBF. HCBF is side polished to remove part of the cladding that the suspended inner surface of the fiber can contact the external environment. After the incident light passes through the MMF from the SMF, it enters the hollow area, high refractive index (RI) layers, respectively, where the anti-resonant reflecting optical waveguide (ARROW) guiding mechanism and Mach-Zehnder interferometer (MZI) are simultaneously excited. Taking advantage of the high RI layers of HCBF, compared to the fiber with uniform cladding, the light can be more confined in the cladding and more sensitive to inner surface medium. The inner surface of sensor is immobilized with cortisol aptamer for the sake of achieving high sensitivity and specific sensing of cortisol with the limit of detection (LOD) to be 4.303 pM. The proposed sensor has a compact structure, enables temperature compensation, and can be fabricated at low cost making it highly suitable for in-situ monitoring and high-precision sensing of cortisol and other biological analytes.
研究领域:
光学
·
生物传感
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Ruifang Liu,Hang Song,Hao Wu,Zhihao Yang,Surong Fang,Jing Zhu,Long Ba
Lung cancer is the main cancer that endangers human life worldwide, with the highest mortality rate. The detection of lung tumor markers is of great significance for the early diagnosis and subsequent treatment of lung cancer. In this study, a vertical graphene field effect transistor (VGFET) immunosensor based on graphene/C 60 heterojunction was created to offer quantitative detections for the lung tumor markers carcinoembryonic antigen (CEA), cytokeratin 19 fragment (Cyfra21-1), and neuron-specific enolase (NSE). The experimental results showed that the sensitive range for standard antigen is between 1 pg/ml to 100 ng/ml, with a limit of detection (LOD) of 5.6 amol/ml for CEA, 33.3 amol/ml for Cyfra 21–1 and 12.8 amol/ml for NSE (1 pg/ml for all). The detection accuracy for these tumor markers was compared with the clinically used method for clinical patients on serum samples. Results are highly consistent with clinically used immunoassay in its efficient diagnosis concentration range. Subsequently, the mesoporous silica nanospheres (MSNs) with an average size of 90 nm were surface modified with glutaraldehyde, and a second antibody was assembled on MSNs, which fixes nanospheres on the antigen and amplified the field effect. The LODs for three markers are 100 fg/ml (0.56 amol/ml for CEA) under optimal circumstances of detection. This result indicates that specific binding to MSNs enhances local field effects and can achieve higher sensing efficiency for tumor marker detection at extremely low concentrations, providing effective assistance for the early diagnosis of lung cancer.
研究领域:
生物医学工程
·
纳米技术
·
癌症诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yumei Chen,Jialin Zhang,RuiRui Liu,Ying Zhang,Jingming Zhou,Hongliang Liu,Yankai Liu,Ke Yan,Yanhua Qi,Enping Liu,Xifang Zhu,Aiping Wang
A label-free electrochemical immunosensor was developed to rapidly detect tilmicosin (TMC) residues in pork and milk. The immunosensor was constructed by immobilizing a high-affinity monoclonal antibody against TMC on an rGO-PEI-Ag nanocomposite-modified electrode. The rGO-PEI-Ag nanocomposites were prepared by mixing polyethyleneimine (PEI) modified reduced graphene oxide (rGO) with AgNO 3 solution. The prepared rGO-PEI-Ag nanocomposites showed good redox activity and conductivity, as characterized by ultraviolet-visible spectroscopy (UV–Vis), transmission electron microscopy (TEM), and X-ray diffraction (XRD). During the preparation process, staphylococcal protein A (SPA) was added to targetedly bind the Fc segment of the monoclonal antibody. The immunosensor showed a low detection limit (LOD) of 0.0013 ng/mL, a linear range of 0.01–100 ng/mL, and recoveries ranging from 92.77 to 100.02% in pork and 92.26–101.23% in milk. Furthermore, the immunosensor exhibited good stability, reproducibility, and specificity in detecting TMC in pork and milk real samples.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Kaijie Ma,Le Liu,Pengfei Zhang,Yonghong He,Qing Peng
An angular plasmonic sensor based on optimized angle-pixel resolution to detect antibody-antigen binding process is presented. Owing to discrete resonance curves by the CCD pixels, the pixel sensitivity and detection noise of the plasmonic sensors need to be balanced. The angle-pixel resolution is adjusted by changing the distance between the sensor chip and the CCD to obtain the optimal refractive index (RI) resolution for three plasmonic sensors, which are surface plasmon resonance (SPR), long range surface plasmon resonance (LRSPR) and plasmon waveguide resonance (PWR) sensors. Each plasmonic sensor can achieve its optimal RI resolution at the optimal distance, and the LRSPR sensor has the best RI resolution. Attributing to the reference channel and temperature control module in the sensor system, the RI resolution can achieve 4.37 × 10 −7 RIU at Normal mode and 1.13 × 10 -7 RIU at a slow response Extreme mode. The Protein A-IgG binding reaction experiment indicates that the plasmonic sensor has linear response to IgG concentration in a wide range and the limit of detection (LOD) is 5.45 pM at Extreme mode. The procedure and result of this work are helpful to the optimization of other angular plasmonic sensors.
研究领域:
生物传感
·
等离子体学
·
光学传感
·
分析化学
·
生物物理学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jintao Liang,Kaiteng Yan,Yutong Liu,Xiaoqing Yao,Fei Guo,Wen Xue,Guiyin Li,Jiejing Chen,Zhide Zhou
1,5-anhydroglucitol (1,5-AG) is an important indicator in the early detection of diabetes. In this study, a high-performance light addressable potentiometric sensor (LAPS) for the detection of 1,5-AG based on reduced graphene oxide-polyacrylamide-ferrocene/gold nanoparticles (rGO-PAM-Fc/AuNPs) as sensing membrane and pyranose oxidase (PROD) as the specific recognition substance was constructed. Among them, rGO-PAM-Fc/AuNPs modification LAPS chip can not only effectively increase the specific surface area and adsorb more PROD , but also enhance the capacitive effect of LAPS and improve the detection sensitivity. 1,5-AG is hydrolyzed by PROD and produces hydrogen peroxide (H 2 O 2 ) that can oxidize divalent ferrocene ions in the rGO-PAM-Fc composite to trivalent ferrocene ions, therefore leads to a change in the membrane potential on the surface of the LAPS chip and causes a shift in the I-V curve, which reflect the change of 1,5-AG concentration. The designed LAPS had a good linearity with 1,5-AG concentration in the range of 100.0–1000.0 μg/mL with a correlation coefficient of 0.99068. The sensitivity was calculated to be 0.1908 mV/μg/mL and a limit of detection (LOD) of 21.74 μg/mL was obtained using three signal-to-noise ratio (S/N = 3). Also, the 1,5-AG biosensor has good specificity, reproducibility and stability, and can be used in human serum samples with the recoveries of 93.62 %–114.52 %. This work provides a highly sensitive, portable and low-cost assay for the detection of 1,5-AG. Prime novelty statement Herein, a high-performance light addressable potentiometric sensor (LAPS) for the detection of 1,5-AG based on reduced graphene oxide-polyacrylamide-ferrocene/gold nanoparticles (rGO-PAM-Fc/AuNPs) as sensing membrane and pyranose oxidase (PROD) as the specific recognition substance was constructed. Among them, rGO-PAM-Fc/AuNPs modification LAPS chip can not only effectively increase the specific surface area and adsorb more PROD, but also enhance the capacitive effect of LAPS and improve the detection sensitivity. 1,5-AG is hydrolyzed by PROD and produces hydrogen peroxide (H 2 O 2 ) that can oxidize divalent ferrocene ions in the rGO-PAM-Fc composite to trivalent ferrocene ions, therefore leads to a change in the membrane potential on the surface of the LAPS chip and causes a shift in the I-V curve, which reflect the change of 1,5-AG concentration. The designed LAPS had a good linearity with 1,5-AG concentration in the range of 100.0–1000.0 μg/mL with a correlation coefficient of 0.99068. The sensitivity was calculated to be 0.1908 mV/μg/mL and a limit of detection (LOD) of 21.74 μg/mL was obtained using three signal-to-noise ratio (S/N = 3). Also, the 1,5-AG biosensor has good specificity, reproducibility and stability, and can be used in human serum samples with the recoveries of 93.62 %–114.52 %. This work provides a highly sensitive, portable and low-cost assay for the detection of 1,5-AG.
研究领域:
生物传感
·
纳米技术
·
分析化学
·
糖尿病检测
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-10
·
IF 6.408
Wang Yuexin,Chen Rui,Shen Bo,Li Cai,Chen Junman,Wang Yanshuang,Tian Shen,Li Xuemiao,Luo Nini,Liu Rui,Ding Shijia,Zhu Chuanlong,Xia Qianfeng
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal–organic frameworks (Co-MOF), gold nanoparticles (AuNP), and N -(4-aminobutyl)- N -(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay of Burkholderia pseudomallei with the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination of B. pseudomallei in serum sample was as low as 9.0 CFU mL −1 . The relative standard deviations (RSD) of ECL intensity for the detection of five B. pseudomallei -spiked serum samples were 4.02%, 0.84%, 0.84%, 1.55%, and 0.21%, respectively. The recoveries of the ECL biosensor for the detection of B. pseudomallei DNA-spiked serum samples were 93.63 ~ 107.83%. Therefore, this work demonstrated that the developed multifunctional catalytic nanomaterial with synergistic and zero-distance catalytic properties can be used as excellent ECL signal reporter to improve the detection sensitivity of ECL biosensor. Graphical abstract
研究领域:
生物传感器
·
分析化学
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Huanhuan Li,Wenhui Geng,Zhixiong Qi,Waqas Ahmad,Suleiman A. Haruna,Quansheng Chen
In this work, a stimuli-responsive SERS biosensor was fabricated for tetracycline (TTC) by “signal-on” strategy using (EDTA)-driven polyethyleneimine grafted calcium carbonate ( [email protected] 3 ) microcapsule and chitosan-Fe magnetic microbeads ( [email protected] ). Initially, aptamer conjugated magnetic-bead [email protected] @Apt with superparamagnetism and excellent biocompatibility was employed as capture probe, which facilitated the rapid and easy magnetic separation. Subsequently, the PEI cross-linked layer and aptamer network layer were constructed onto the outer layer of CaCO 3 @4-ATP microcapsule to form sensing probes ( [email protected] 3 @ [email protected] ) via the layer-by-layer assembly method. In the presence of TTC, a sandwich SERS-assay was exploited by aptamer recognition induced target-bridged strategy. When the solution of EDTA was added, the core layer of CaCO 3 would be dissolved quickly, destroying the microcapsule to release 4-ATP. The released 4-ATP could be quantitatively monitored by dripping the supernatant onto the [email protected] SERS platform, resulting in a strong Raman “signal-on”. Under the optimal conditions, a good linear relationship was established with a correlation coefficient ( R 2 ) of 0.9938 and a LOD of 0.03 ng/mL. Additionally, the application capacity of the biosensor to detect TTC was also affirmed in food matrixes, and the results were consistent with the standard ELISA method ( P > 0.05). Hence, this SERS biosensor affords extensive application prospects for TTC detection with multiple merits such as high sensitivity, environment friendliness, and high stability.
研究领域:
分析化学
·
环境科学
·
食品安全
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）