Chinese Herbal Medicines
·
2026
·
IF 11.2
Jiahe Zhang,Keyun Liu,Jinyao Tang,Linlin Wang,He Xiao,Jingyi Zhang,Xihe Yang,Jingwen Wang,Siyuan Lu,Yuanzhen Cao,Shanshan Sun,Chunxue Zhao,Yungang Tian,Yuxi Huang,Yang Zhang,Jianbo Wan,Xue Qiao
Objective Lycii Cortex (Digupi in Chinese) (root bark of Lycium barbarum ), a widely used traditional Chinese medicine, accumulates bioactive phenolamides named kukoamines. The enzymes catalyzing kukoamine formation in L. barbarum remain unknown. This study aims to characterize the key coenzyme A (CoA) ligase and BAHD acyltransferase (a plant-specific acyltransferase family) that catalyze formation of kukoamine A. Methods Candidate genes were identified using BLAST and phylogenetic analysis. Catalytic functions and substrate specificities were characterized through recombinant protein expression, in vitro enzymatic assays, and chromatographic and mass spectrometric analyses. Molecular modeling and site-directed mutagenesis were performed to identify key residues involved in catalysis. Results Three BAHD acyltransferases (BAHD-ATs), L. barbarum acyltransferase 1/6/7 (LbAT1/6/7), along with a dihydrocaffeic acid - coenzyme A ligase, L. barbarum CoA ligase 1 (Lb4CL1), were identified. LbAT1/6/7 represent the first functionally characterized dihydrocaffeoyl-CoA: spermine acyltransferases that catalyze kukoamine A biosynthesis. Phylogenetic analysis revealed that they showed distinct phylogenetic divergence from most characterized polyamine BAHD-ATs. Further mining of the One Thousand Plant Transcriptomes Project (1KP) database identified a lineage-specific expansion of these polyamine BAHD-AT genes within the Solanaceae family. LbAT6 exhibited broad substrate promiscuity, accepting multiple polyamine substrates and diverse hydroxycinnamoyl-CoA derivatives as acyl donors. Structural modeling and site-directed mutagenesis identified critical residues governing the catalytic activities of LbAT6 and Lb4CL1. Conclusion This study identified Lb4CL1 and LbAT1/6/7 as the key enzymes catalyzing the formation of kukoamine A, and laid a foundation for the production of kukoamine A as well as its derivatives.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Jingjing Liang,Xiaohan Li,Wenzhi Lei,Pei Tan,Mengzhen Han,Hongcai Li,Tianli Yue,Zhouli Wang,Zhenpeng Gao
Gut microbes and microbial metabolites derived from polysaccharides mediate beneficial effects related to polysaccharides consumption. Lycium barbarum polysaccharide (LBP) is the main bioactive components in L. barbarum fruits and possesses considerable health-promoting effects. In the present study, we aimed to investigate whether LBP supplementation influenced host metabolic responses and gut microbiota in healthy mice, and to identify bacterial taxa associated with the observed beneficial effects. Our results indicated that mice supplied with LBP at 200 mg/kg BW showed lower serum total cholesterol (TC), triglyceride (TG), and liver TG levels. LBP supplementation strengthened the antioxidant capacity of liver, supported the growth of Lactobacillus and Lactococcus , and stimulated short-chain fatty acids (SCFAs) production. Serum metabolomic analysis revealed that fatty acid degradation pathways were enriched, and RT-PCR further confirmed that LBP up-regulated the expression of liver genes involved in fatty acid oxidation. The Spearman’s correlation analysis indicated that some serum and liver lipid profiles and hepatic SOD activity were associated with Lactobacillus , Lactococcus , Ruminococcus , Allobaculum and AF12 . Collectively, these findings provide new evidence for the potential preventive effect of LBP consumption on hyperlipidemia and nonalcoholic fatty liver disease.
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PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Na Zhang,Xue Han,Rumeng Yu,Xiaomin Tang,Bo Zhang,Yanyan Zhang,Xiaoyu Yin,Wenrui Ni,Mengze Wang,Bolin Zhang,Junfeng Fan
A bile salt (BS)-stabilized water/oil interface is a prerequisite for the pancreas lipase (PL) mediated hydrolyzation of triacylglycerol. Previous fat digestion research mainly focused on direct lipase inhibition by bioactive substances while ignoring the destructive role of these substances on BS-stabilized lipid emulsions . Here, we showed that polysaccharides (LP) weakly suppressed PL but destroyed BS-stabilized emulsions and thus inhibited free fatty acid (FFA) release. Physicochemical analysis showed that LP actions toward emulsion stability were mainly due to endogenous minerals (28%) in LP which affected aggregation and amphiphilic properties of BS-polysaccharide composites. This was evidenced by high wettability and low interfacial tension in demineralized polysaccharide and BS composites, with three phase contact angles which were identified as 80.4° and 95.6° as well as interfacial tensions as 9.26 and 9.37 mN/m, respectively. However, the low wettability, which was detected as 76.2° and 78.6° as well as high interfacial tension as 10.77 and 11.02 mN/m, respectively, was only observed in mineral-containing polysaccharides and BS composites. Further investigations showed that interactions with mineral-containing polysaccharides occupied hydrophobic sites in BS thereby elevating composite hydrophilicity. These findings provided novel insights into the effects of plant-sourced polysaccharides on fat digestion from an interface chemistry perspective, and in particular, they deepened our understanding of polysaccharide interactions with intestine-secreted lipid digestants.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Zhonghai Tang,Yuan Xu,Changyong Cai,Zhijian Tan
Due to the nonvolatility of deep eutectic solvents (DESs), the recycling and reuse of them is encountering a lot of problems in the extraction and separation field. In this study, temperature-switchable DESs (TS-DESs) were prepared and their temperature-responsive characteristics and phase diagram were studied. TS-DESs were further used as the recyclable extractants for extracting Lycium barbarum polysaccharides (LBP) below their lower critical solution temperature (LCST), and then they can be recovered from the extract above their LCST. The main factors influencing the extraction process were investigated in the single-factor experiments. A maximal extraction yield of 465 mg/g was obtained. The TS-DES was recovered and reused in recycling experiments. The recovery of TS-DES was 80.2 % after the fifth cyclic test. The obtained LBP was studied by determining its molecular weight, assessing its radical scavenging activity, and analyzing its monosaccharide constituents. This study presents a novel method for polysaccharide extraction, which can also provide guidance for bioactive compounds extraction using recyclable extractants.
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高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Zhiyong Guo,Zilin Wang,Yige Liu,Hao Wu,Qiaoyu Zhang,Jing Han,Jiannan Liu,Chenping Zhang
Radiation-induced bone injury management remains a challenge in clinical practice, and there is no effective medicine. Recently, biomass-derived carbon dots (CDs) have attracted attention in biomedical engineering due to the advantages of abundant heteroatoms, low toxicity, and no need to drug loading. Here, we report that CDs, synthesized from Lycium barbarum via hydrothermal strategy, can effectively alleviate radiation-induced bone injury. CCK-8, apoptosis analysis, β-galactosidase staining, quantitative polymerase chain reaction, and western blots demonstrate that CDs can mediate radiation-induced damage and senescence of bone marrow mesenchymal stem cells (BMSCs). CDs regulate osteogenic- and adipogenic-balance after irradiation, shown by alizarin red and oil red O staining. In vivo experiments reveal that CDs prevent the occurrence of osteoradionecrosis in rats, demonstrated by micro-CT and histology examination. The osseointegration of titanium implants installed in irradiated bone is promoted by CDs. Mechanistically, CDs increase the N6-methyladenosine (m6A) level of irradiated BMSCs via the increased methyltransferase-like 3 (METTL3). High-throughput sequencing facilitates detection of increased m6A levels located in the 3′-untranslated regions (UTR) of the CAP-Gly domain containing linker protein 3 (Clip3) mRNA. The dual-luciferase reporter assay shows that 3′UTR is the direct target of METTL3. Subsequently, the increased m6A modification led to enhanced degradation of mRNA and downregulated CLIP3 expression, eventually resulting in the alleviation of radiation-induced bone injury. Interfering with the METTL3/Clip3 axis can antagonize the effect of CDs, indicating that CDs mediate radiation-induced bone injury via the METTL3/Clip3 axis. Taken together, CDs from L. barbarum alleviate radiation-induced bone injury by inhibiting senescence via regulation of m6A modification of Clip3. The present study paves a new pathway for the management of radiation-induced bone injury.
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PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Lei Ding,Xuan Zhou,Xiaojie Liang,Yujin Dong,Cunbao Fang,Yueming Wu,Beixing Li,Wei Mu,Jin Lin,Yuekun Li
Pesticide residue has become the main technical barrier that restricts the export of Chinese wolfberry. Can we achieve high efficacy and low safety risk by balancing pesticide deposition on the leaves and fruits of Chinese wolfberry? In this research, the structural characteristics and wettability of leaves and fruits of Chinese wolfberry at different growth stages were studied. The adaxial and abaxial surfaces of leaves were hydrophobic, whereas the fruit surfaces were hydrophilic. Adding spray adjuvant could increase the retention of droplets on the leaf surfaces of Chinese wolfberry by 52.28–97.89% and reduce the retention on the fruit surfaces by 21.68–42.14%. A structural equation model analysis showed that the adhesion tension was the key factor affecting the retention of the solutions among various interface behaviors. When the concentrations of Silwet618, AEO-5, Gemini 31551, and 1227 were 2–5 times higher than their CMCs, the retention of pesticide solutions (pyraclostrobin and tylophorine) on Chinese wolfberry leaves significantly increased, and the control efficacies on aphids and powdery mildew also dramatically improved (65.90–105.15 and 41.18–133.06%, respectively). Meanwhile, the retention of pesticides on the fruit of Chinese wolfberry was reduced. This study provides new insights into increasing the utilization of pesticides in controlling pests and improving food safety.
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Rui Wang,Jian Zhang,Zhengyi Sun,Xuewen Jian,Yue Xu,Xuan Zhou,Xiaojie Liang,Jin Lin,Beixing Li,Wei Mu,Yuekun Li
Realizing eco-friendly, long-term, and low-risk aphid control on Lycium barbarum (medicinal cash crop) using a Cynanchum komarovii extracts and eucalyptus oil-loaded microcapsules (EOMCs) formulation compositions is viable. In this study, the aim is to optimize the composition of Cynanchum komarovii extracts and EOMCs formulation for effective control of aphids, the release of EOMCs was controlled by changing the cross-linking degree of the shell to match the aphid control characteristics of Cynanchum komarovii extracts. Four types of polyamines were used as cross-linking agents for the preparation of EOMCs by interfacial polymerization. The bioactivity, wettability, and field application efficacy of Cynanchum komarovii extracts and different EOMCs formulation compositions were evaluated. These EOMCs exhibited an encapsulation efficiency exceeding 85 %. The control efficiency of the formulation compositions of microcapsules with a moderate release rate and Cynanchum komarovii extracts on aphids remained at 62.86 %, while the control efficiency of the combination of microcapsules with the fastest and slowest rates with Cynanchum komarovii extracts was only 48.62 % and 57.11 %, respectively. The formulation compositions of Cynanchum komarovii extracts with all four types of EOMCs were found to be safe for Chinese wolfberry plants. Overall, by selecting appropriate polyamines during fabrication, the release rate can be effectively controlled to achieve sustainable and low-risk aphid control in Lycium barbarum through compounding with selected microcapsules.
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高影响因子
Food & Function
·
2019
·Vol. 10
·期 6
·3671-3683
·
IF 3.241
Yu Ding,Yamei Yan,Dan Chen,Linwu Ran,Jia Mi,Lu Lu,Bo Jing,Xiaoying Li,Xiaoxiong Zeng,Youlong Cao
In the present study, the effects of Lycium barbarum polysaccharides (LBPS) on immunoregulation and gut microbiota dysbiosis in cyclophosphamide (CTX)-induced mice were investigated to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota. The results showed that administration of LBPS could protect immune organs (enhancing immune organ indexes and alleviating immune organ damage), enhance the production of immune-related cytokines (IL-2, IL-6, IL-1β, TNF-α and IFN-γ) and prevent the hepatotoxicity in CTX-induced mice. Additionally, LBPS treatment could promote the production of short-chain fatty acids and modulate the composition of the gut microbiota, increasing the relative abundances of Bacteroidaceae, Lactobacillaceae, Prevotellaceae and Verrucomicrobiaceae, which were positively associated with immune traits. The present results indicated that LBPS might regulate the immune response depending on the modulation of the gut microbiota, suggesting that LBPS could be developed as special ingredients for immunoregulation in association with the modulation of the gut microbiota.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Yun Wang,Xiaowei Hu,Juan Han,Liang Ni,Xu Tang,Yutao Hu,Tong Chen
A polymer–salt aqueous two-phase system (ATPS) consisting of thermosensitive copolymer ethylene-oxide-b-propylene-oxide-b-ethylene-oxide (EOPOEO) and NaH 2 PO 4 was employed in deproteinization for lycium barbarum polysaccharide (LBP). The effects of salt type and concentration, EOPOEO concentration, amount of crude LBP solution and temperature were studied. In the primary extraction process, LBP was preferentially partitioned to the bottom (salt-rich) phase with high recovery ratio of 96.3%, while 94.4% of impurity protein was removed to the top (EOPOEO-rich) phase. Moreover, the majority of pigments could be discarded to top phase. After phase-separation, the LBP in the bottom phase was further purified by dialysis membrane to remove salt and other small molecular impurities. The purity of LBP was enhanced to 64%. Additionally, the FT-IR spectrum was used to identify LBP. EOPOEO was recovered by a temperature-induced separation, and reused in a new ATPS. An ideal extraction and recycle result were achieved.
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高影响因子
Frontiers in Pharmacology
·
2026
·
IF 5.4
Yuanyuan Ge,Wei Chen,Lingling Jiang,Kunhui Sun,Guojing Liu,Yanfeng Liu,Yibao Jin,Ping Wang,Liang Zhang,Mingtong Zhang,Lan Ma,Xie-An Yu,Bing Wang
Introduction Sulfur fumigation is a traditional technique for drying, pest control and mildew prevention in traditional Chinese medicine (TCM). However, excessive sulfur fumigation significantly impairs drug safety and clinical efficacy, rendering sulfur dioxide (SO 2 ) residues in sulfur-fumigated TCM a growing concern. Conventional detection methods exhibit drawbacks in practical operation, such as complex procedures, long time consumption, low sensitivity and expensive instruments. Methods Herein, a fluorescent probe-based method was established for the rapid and accurate detection of SO 2 residues in wolfberry, leveraging the nucleophilic addition-elimination reaction between the probe and sulfite. Considering the practical application requirements for the quality testing of TCM, the pretreatment parameters including sample forms, extraction modes, extraction times with extraction solvent were optimized. The detection protocol was finalized as follows: samples were soaked for 15 min in HEPES buffer (20 mM, pH 7.4), and the extract was reacted with the fluorescent probe at 35 °C for 15 min, followed by detection on a microplate reader. Results The detection limit (LOD) was 1.5 μM, and the limit of quantification (LOQ) was 5 μM. The average recovery rates at low, medium, and high spiked concentrations ranged from 89.5% to 100.8%, the corresponding detection method was further applied to actual samples. Discussion Accordingly, this method features facile pretreatment, rapid operation and detection, high accuracy and sensitivity, enabling rapid and reliable determination of SO 2 residues in wolfberry. It provides a novel technical tool for TCM quality supervision and offers technical support for safeguarding public health.
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PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Qian Wang,Xuan Liu,Daohang Xing,Lihan Jian,Zhichun Yang,Xiaoqin Wang,Linjuan Huang,Zhongfu Wang
Complex-structured pectin polysaccharides are efficient natural food-grade emulsifiers and stabilizers. However, the relationship between the structure features of pectin polysaccharides from different plant sources and their emulsifying properties remains unclear, limiting the fabrication of stable pectin emulsions and their application. In this study, pectin polysaccharides were extracted from sunflower disk, beet root, apple pomace, grapefruit peel and citrus peel. Of the five polysaccharides, citrus pectin (CP) was found to exhibit the best emulsifying performance, which was attributed to its higher content of polysaccharides-covalently bound protein, higher degree of methyl-esterification, and higher branching degree in RG-I region. CP emulsions with varying CP concentrations (c) and oil volume fractions (φ) were prepared and analyzed for the structure and stability. When φ ≤ 30%, 2.0% CP emulsion with smaller particle size exhibited higher storage stability. When φ > 30%, the stability of 1.0% CP emulsion was relatively higher. In particular, c2.0%-φ20% emulsion exerted the highest stability against heating and ionic strength, which also had a high encapsulation efficiency (84.09% ± 0.53) for Lycium barbarum extract (LBE). LBE-loaded CP emulsion showed no cytotoxicity, and demonstrated higher antioxidant activity and inhibition rates against tyrosinase, elastase and hyaluronidase activities, which holds promise as a novel anti-aging emulsion for cosmetic application.
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PubMed
高影响因子
Food Quality and Safety
·
2020
·Vol. 4
·期 4
·193-201
·
IF 0
Liu Bei,Xu Qingqing,Sun Yujing
Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.
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危险品化学品经营许可证（带存储）