BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Xiaoru Zhang,Mingshuai Liu,Hongxia Liu,Shusheng Zhang
A new photoelectrochemical (PEC) biosensor was developed using low-toxic Ag 2 S QDs as photoelectrochemically active species. Energy levels of Ag 2 S and Ag 2 Se QD were compared to explain their differences in the PEC performance. The preparation condition of Ag 2 S QD was optimized and its structure characterization was measured. Then the developed photoelectric active interface was used to detect glucose and MCF-7 cancer cell and showed the good sensitivity and specificity. Under optimal condition, detection limits of 3.2×10 −5 M for glucose and 98 cells/mL for MCF-7 cell were achieved. Thus, the prepared Ag 2 S QD could serve as an excellent and promising photoelectric active material in the PEC biosensor.
研究领域:
生物传感技术
·
材料科学
·
医学诊断
✓ 已验证
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Xiaoye Yang,Xiaoqun Shi,Yanan Zhang,Jiangkang Xu,Jianbo Ji,Lei Ye,Fan Yi,Guangxi Zhai
To improve the anti-cancer therapeutic effect of nanosystems for chemo-photodynamic therapy, there remain several hurdles to be addressed, e.g. , limited co-loading efficiency, insufficient stimulus-responsiveness and lack of synergetic effect. This work reported novel reactive‑oxygen-species (ROS)-responsive chlorin e6 (Ce6) and paclitaxel (PTX) co -encapsulated chondroitin sulfate- g -poly (propylene sulfide) nanoparticles (CP/ChS- g -PPS NPs), wherein the drug loading efficiencies of Ce6 and PTX were as high as 14.93% and 24.31%, respectively. To enlarge the ROS signal at tumor sites thus enhancing the ROS-responsiveness of ChS- g -PPS NPs, near-infrared (NIR) light was utilized to induce Ce6 to produce more ROS to destruct the NPs. Our data showed that the photo-triggered self-destructive property of NPs helped drugs to spread deeper in tumors upon laser irradiation, making the NPs promising to thoroughly remove tumor cells. CP/ChS- g -PPS NPs exhibited a synergetic chemo-photodynamic therapy effect in vitro , which was suggested by the combination indexes of PTX and Ce6 lower than 1 when 20–80% inhibition rates of MCF-7 cells were achieved. As for the in vivo antitumor activity , the tumor inhibition rates of CP/ChS- g -PPS NPs (with laser irradiation) were as high as 92.76% and 88.57% in 4T1 bearing BALB/c mice and MCF-7 bearing BALB/c nude mice, respectively, which were significantly higher than those of other treatment groups. This work provided a simple yet effective strategy to develop photo-triggered ROS-responsive NPs for synergetic chemo-photodynamic therapy with quick ROS-responsive self-destruction, spatiotemporally controllability, reduced off-target toxicity, and desirable therapeutic effect.
研究领域:
药学
·
纳米技术
·
癌症治疗
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Yang Shu,Na Zheng,An-Qi Zheng,Ting-Ting Guo,Yong-Liang Yu,Jian-Hua Wang
Fluorescence imaging of cellular metals is widely reported. However, the quantification of intracellular metals with fluorescence imaging is so far not feasible and highly challenging. In this work, a ratiometric probe with two fluorescently labeled complementary DNA strains is designed for intracellular zinc quantification via fluorescence imaging, based on fluorescence resonance energy transfer (FRET) from carbon dots (CDs) to fluorescein (FAM). The donor CDs are modified with a Zn2+ aptamer, whereas the receptor FAM is conjugated with the complementary DNA sequence to ensure selectivity. MCF-7 cells are cultured sequentially with Zn2+ (20, 40, 55, 70, 85, and 100 μmol L–1) and CDs–FAM (100 μg mL–1), which is used for fluorescence imaging (at λex = 405 nm and λem = 440–490 nm for CDs, λem = 500–550 nm for FAM) to provide a relative fluorescence ratio ((F – F0)/F0, F = ICDs/IFAM), followed by quantifying intracellular zinc with ICPMS. A linear correlation is achieved between the relative fluorescence ratio in fluorescence images and the intracellular zinc content derived by ICPMS, which facilitates intracellular zinc quantification via fluorescence imaging. It is especially useful for real-time tracing of intracellular zinc during the cell culturing process or in vivo. The cellular uptake of Zn2+ by MCF-7 cells is further evaluated with this approach by culturing with 100 μmol L–1 of Zn2+ for different times, and a maximum uptake of 60.5 fg per cell is observed at an incubation time of 60 min. This value is further demonstrated well by ICPMS detection.
研究领域:
分析化学
·
细胞生物学
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生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Shi Zhou,Peng Guo,Jinlian Li,Lingren Meng,Hongfu Gao,Xing Yuan,Dongmei Wu
Fluorene was one of the priority controlled polycyclic aromatic hydrocarbons (PAHs) and distributed abundantly in the environment, which has a great harmful on human body. It was very important to detect the cytotoxicity of fluorene with a rapid and accurate method. In this present work, a simple and ultrasensitive electrochemical biosensor was developed by electrodeposition of reduced graphene oxide quantum dots (RGOQDs) on glassy carbon electrode (GCE) for the first time. The electrochemical behaviors of uric acid (UA), xanthine (X) and guanine (G) were studied on RGOQDs/GCE by differential pulse voltammetry (DPV) with a limit of detection of 0.024 μM, respectively. Then the electrochemical biosensor was used to evaluate the cytotoxicity of fluorene for MCF-7 cells based on the variation of G/X in MCF-7 cells solution. The result showed that fluorene exposed to MCF-7 cells at the concentration range of 0–0.05 mM could promote cell proliferation, the concentration above 0.05 mM exhibited inhibitory effect, which was verified by methyl-thiazolyl-tetrazolium (MTT) assay. The electrochemical biosensor based on RGOQDs/GCE could be used not only for evaluation the cytotoxicity of PAHs, but also for detection the physiological process related to intracellular purine nucleotide metabolism.
研究领域:
生物医学工程
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环境科学
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 26
·12788-12796
·
IF 7.233
Shuang E,Quan-Xing Mao,Xiao-Li Yuan,Xiao-Lei Kong,Xu-Wei Chen,Jian-Hua Wang
Organelles play crucial roles in cellular activities and the functions of organelles are related greatly to the pH values, therefore, the bio-imaging of targeted organelles and their related pH sensing is of great importance in biological assays. Herein we report the fluorescence imaging of specific organelles, i.e., lysosomes and endoplasmic reticulum, and their pH sensing with surface regulated carbon dots (CDs). Carbon dots functionalized with amine groups (ACDs) are first prepared by hydrothermal treatment of citric acid and urea, and then laurylamine functionalized CDs (LCDs) are obtained via the conjugation of laurylamine with ACDs. The as-prepared ACDs and LCDs provide clear and bright imaging results for the lysosome and endoplasmic reticulum, respectively. The subcellular targeting features of the two CDs are attributed to their surface chemistries and cellular uptake pathways. Moreover, both the CDs are pH responsive within a certain pH range, i.e., 4.0–5.4 for ACDs and 6.2–7.2 for LCDs. The ACDs and LCDs are thus successfully applied to visualize the pH fluctuations of the lysosome and endoplasmic reticulum in MCF-7 cells.
研究领域:
细胞生物学
·
生物医学工程
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2019
·Vol. 21
·期 1
·1-10
·
IF 2.608
Yang Yue,Li Jinyu,Chen Fen,Qiao Sen,Li Yunjian,Pan Weisan
As a synthetic clay material, laponite RDS (LR) was investigated as an effective drug carrier as a result of the special nanodisk structure together with the negative-charged surface to achieve enhanced cellular uptake and targeted delivery. In this research work, the synthesized oligomeric hyaluronic acid-aminophenylboronic acid (oHA-APBA) was entangled onto LR nanodisks to fabricate a valid targeted platform for breast cancer therapy. Briefly, through the formation of amide bonds, 3-APBA was connected to the chain of oHA with a substituted ratio of 4.0 ± 0.2% to synthesize oHA-APBA copolymer. Thereafter, doxorubicin (DOX) was inserted into the interlayer space of LR by the way of the ion exchange process, followed by an assembly with oHA-APBA as a targeted protection layer. The satisfactory drug encapsulation efficiency (> 80%) and narrow size distribution were achieved. The in vitro drug release study demonstrated the release of DOX from DOX@LR/oHA-APBA was sustained and acid dependent. In addition, after fitting the drug cumulative release of DOX@LR/oHA-APBA under different pH conditions with several kinetic models, it was identified that drug release from DOX@LR/oHA-APBA nanohybrids at pH 5.0 was mainly dependent on both diffusion and ion exchange effects. However, under the condition of pH 7.4, the drug was most efficiently released by diffusion effect. Importantly, DOX@LR/oHA-APBA showed remarkable cellular uptake and intracellular drug distribution in MCF-7 cells, which were consistent with inhibitory ability against MCF-7 cells. Hence, the high DOX loading capacity and enhanced cellular tracking can enlighten LR/oHA-APBA as an effective drug delivery carrier for breast cancer therapy.
研究领域:
药剂学
·
肿瘤学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Di Lu,Xinyue Zheng,Hongyu Xue,Jiansong You,Lei Yin,Meiyun Shi
The aim of this work was to develop a fast, simple, and reliable UPLC-MS 3 method for the sensitive detection of acetochlor in biological samples. In MS 3 mode, the ion transition m/z 270.1 → 224.1→148.1 was chosen for quantification with butachlor as the internal standard. In the UPLC system, separation was performed on a UPLC column (2.1 × 50 mm ID, 1.7 μm) with 0.1 % FA in water and acetonitrile as mobile phases . After simple protein precipitation via acetonitrile, the method was well validated with good linearity (0.5–20 ng/mL, r > 0.995), accuracy (−3.70 %-2.98 %), and precision (<15 %). The selectivity and sensitivity were improved obviously in MS 3 mode than that in MRM mode. The developed UPLC-MS 3 method was successfully applied to the cellular pharmacokinetics study of acetochlor in MCF-7 cells.
研究领域:
分析化学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Ji-Wen Cui,Tie-Jun Hou,Qian Wang,Guang-Gang Gao,Sheng Bi,Kui-Chen Zhou,Jin-Lian Li,Dong-Mei Wu
Monitoring purines intracellular quickly and accurately is critically important for revealing physiological processes relate to purine metabolism. However, the overlapped signals of guanine (G) or xanthine (X) and adenine (A) or hypoxanthine (H) hinder the accurate detection of individual purine change in cells. In this paper, a novel electrochemical detecting system has been constructed by introducing xanthine oxidase, which can catalyze H or X to uric acid during the detection process. Using this enzyme-assisted electrochemical detecting system, the individual concentrations of G, X, A and H in the Human breast cancer (MCF-7) cells can be detected simultaneously without any pretreatment. As a result, more information about the purine metabolism in cells can be provided. The cytotoxicity of cyclophosphamide on MCF-7 cell activity was also evaluated by this system, and the results are consistent with that of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which paves a new way to the accurate detection of purines intracellular with the assistance of xanthine oxidase.
研究领域:
生物化学
·
医学检测
·
肿瘤学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Ruoning Wang,Mengmeng Yang,Guoyuan Li,Xin Wang,Zhenhai Zhang,Hongzhi Qiao,Jun Chen,Zhipeng Chen,Xiaobin Cui,Junsong Li
Paclitaxel-betulinic acid hybrid nanosuspensions (PTX-BA-NP) with increased anti-breast cancer activity were developed. The resultant nanosuspensions (NP) had a mean particle size of 282.54 ± 5.4 nm, a polydispersity index of approximately 0.242 ± 0.02, a zeta potential of -19.7 ± 0.19 mV and a redispersibility index of 103.3 ± 0.01%. The cumulative dissolution percentage of PTX coarse powder and PTX-BA-NP dried powder at 60 min were 15.4% and 90.8%, respectively. MCF-7 cell-based testing showed that treatment with PTX-BA-NP led to more PTX-BA-NP accumulation in the cytoplasm of breast cancer cells, less cell cycle arrest in the G2-M phase, more cell cycle arrest in the G0-G1 phase, more apoptosis-induced cell death and stronger inhibition of cell migration than paclitaxel nanosuspensions (PTX-NP). Biodistribution studies showed that tumor accumulation levels at 12 h in the PTX-BA-NP group were approximately 2.67- and 2.33-fold higher than the levels in the Taxol® and PTX-NP groups, respectively. In vivo antitumor efficacy demonstrated that PTX-BA-NP exerted the strongest tumor inhibition among the four groups, with a tumor inhibition rate of 47.79 ± 2.28%, followed by PTX-NP (35.05 ± 5.55%), Taxol® (22.67 ± 6.01%) and betulinic acid nanosuspension (BA NP) (14.38 ± 6.02%). These findings indicate that PTX-BA-NP holds great promise for breast cancer therapy.
研究领域:
药学
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 36
·16822-16832
·
IF 3.591
A-Liang Li,Zhong-Long Wang,Wen-Yan Wang,Qing-Song Liu,Yue Sun,Wen Gu
In this paper, a turn-on fluorescent probe (DBE) was designed and synthesized for probing bisulfite (HSO3−) by coupling dehydroabietic acid-based benzimidazole derivatives with ethyl cyanoacetate moiety. The structure of the compound was characterized using its UV-Vis, 1H-NMR, 13C-NMR and HRMS spectra. The probe showed significant selectivity and sensitivity towards HSO3− compared to other analytes in DMF/PBS buffer (3/7, v/v, 10 mM, pH = 7.4), and exhibited a detection limit at the nanomolar level (3.2 nM), fast response time (140 s) and good pH stability (6–10) in living systems. Furthermore, this probe was successfully utilized for the fluorescence imaging of HSO3− in living zebrafish and MCF-7 cells with remarkable lysosome-targeting properties.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Xiu-Qing Song,Lin-Lin Tian,Tao Ye,Hu Liu,Hua Zhang
ABSTRACT Twenty compounds comprising four pregnane steroids ( 2 – 4 & 20 ) and 16 pregnane glycosides ( 1 & 5 – 19 ) have been obtained from the ethanol extract of the roots of a Dai ethnological herb, Marsdenia tenacissima . Their structures were characterized on the basis of comprehensive spectroscopic analyses with 17 ones ( 1 – 17 ) being reported for the first time, including the rare cases ( 2 & 3 ) of free C 21 steroids with 17α-acetyl substitution, compounds 4 – 7 bearing an unusual 14α-OH, and the first examples with simultaneous 14α-OH/17α-acetyl substitution ( 7 ) and glycosylation at C-12 position ( 10 & 11 ). An empirical rule for the identification of C-17 configuration, in C 21 steroids incorporating the marsdenin constitution structure, was also proposed. All the isolates, along with an array of previously reported analogues in our compound library, were screened for their chemo-reversal ability against P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) in MCF-7/ADR cell line, and six compounds exhibited moderate MDR reversal activity with reversal folds ranging from 1.92 to 4.44.
研究领域:
药理学
·
化学
·
民族药理学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 28
·15831-15841
·
IF 2.936
Li Zong,Zifeng Pi,Shu Liu,Zhiqiang Liu,Fengrui Song
The comprehensive characterization of metabolome and lipidome to reveal unknown pathological conditions, are being used to investigate the molecular mechanisms of cancer, especially in the field of early diagnosis, treatment, and prognosis. The multidrug resistance (MDR) of tumor cells limits the therapeutic effect of anti-cancer drugs and is the main obstacle for chemotherapy. Here, we adopted a methyl-tert-butyl ether (MTBE)-based extraction method to simultaneously extract small polar molecules and lipophilic metabolites for nontargeted metabolomics of multidrug-resistant breast cancer cell line MCF-7/ADR and its parental cell line MCF-7/S by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Distinctly different metabolic features were shown between MCF-7/ADR cells and MCF-7/S on the basis of multivariate analyses. 17 potential biomarkers were identified. And these potential biomarkers were mainly correlated with cell membrane lipids composition, cell signaling regulated by lipids, and anti-oxidation ability. The studies of cellular ultrastructure and morphology by in situ atomic force microscopy (AFM) also demonstrated the cellular membrane changed along with the MDR. We expect that this study could provide a new method for monitoring drug resistance during clinical chemotherapy and be useful for the development of drugs to overcome the MDR.
研究领域:
癌症生物学
·
药理学
·
分析化学
·
细胞生物学
·
生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）