MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 6
·1-8
·
IF 5.705
Li Shuhuai,Li Jianping,Luo Jinhui,Xu Zhi,Ma Xionghui
An electrochemical microfluidic chip is described for the determination of the insecticide carbofuran. It is making use of a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. The analyte (carbofuran) is transported to the MIP and captured at the identification site in the channel. Then, carbofuran is eluted with carbinol-acetic acid and transported to the DNA aptamer on the testing position of the chip. It is captured again, this time by the aptamer, and detected by differential pulse voltammetry (DPV). The dual recognition (by aptamer and MIP) results in outstanding selectivity. Additionally, graphene oxide-supported gold nanoparticles (GO-AuNPs) were used to improve the sensitivity of electrochemical detector. DPV response is linear in the 0.2 to 50 nM carbofuran concentration range at a potential of −1.2 V, with a 67 pM detection limit. The method has attractive features such as its potential for high throughput, high degree of automation, and high integration. Conceivably, the method may be extended to other analytes for which appropriate MIPs and aptamers are available.
Schematic of an electrochemical microfluidic chip for carbofuran detection based on a molecularly imprinted film (MIP) and a DNA aptamer as dual recognition units. In the chip, targets were recognized by MIP and aptamer, respectively. It shows promising potential for the design of electrochemical devices with high throughput, high automation, and high integration.
研究领域:
分析化学
·
电化学
·
环境科学
·
微流控技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Cheng Zeng,Yan Wang,Tong Xiao,Zhicheng Yan,Jinquan Wan,Quanmo Xie
To improve the efficacy of organic pollutant removal using sulfate radicals, we designed MIP@C-Fe-Nx, a molecularly imprinted material capable of targeting the degradation of tetrabromobisphenol A (TBBPA), which can be used as both adsorbent and catalyst to recognize and degrade Tetrabromobisphenol A (TBBPA) accurately, and the final removal rate of TBBPA can reach 104.6 mg·g −1 . Based on the synergistic effects of MIP@C-Fe-Nx on the excellent organic pollutant recognition and catalytic performance, low concentrations of TBBPA can be pre-targeted, concentrated, and fixed on the surface of MIP, and degraded simultaneously in-situ by·OH and SO 4 •- which are produced by activating PS with C-Fe-Nx. Recognition experiments demonstrated that MIPs had perfect performance in recognizing and adsorbing TBBPA and debromination intermediates. The DFT calculations and HPLC-MS analysis indicated that MIP@C-Fe-Nx had a targeted recognition and accumulation for TBBPA and debromination intermediates, for example, dibromobisphenol A, monobromobisphenol A, and bisphenol A, thus avoid the formation of toxic intermediates causing secondary contamination.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Qiuhua Zhang,Youjia Wu,Pingping Wu,Liying Huang,Lingyi Huang
Luteolin is one of the bioactive components from the compound Anoectochilus roxburghii (Wall.) Lindl. oral liquid (CAROL), which was reported to have excellent hepatoprotective and anti-inflammatory activities. However, the enrichment and quantitation of luteolin from CAROL is challenging due to the low content and complex aqueous matrix. In this study, a bifunctional monomer surface molecularly imprinted polymer (MIP) with metal–organic frameworks (MOFs) as cores was prepared for the selective adsorption of luteolin from the aqueous system CAROL. Compared with conventional MIPs, this unique nanocomposite adsorbent (MOF@MIPs) has the advantages of short kinetic equilibrium time, good selectivity, and high adsorption capacity in aqueous solution. The theoretical maximum adsorption capacity of MOF@MIPs for luteolin was 36.99 mg/g. After adsorption enrichment of luteolin from CAROL using MOF@MIPs, liquid chromatography–tandem mass spectrometry was applied to analyze the target. The corresponding linearity range for analyte was 10–6000 ng/mL with good linearity (R 2 =0.9992), and the added recoveries varied from 85.70% to 99.25%. The present method has been successfully employed for the analysis of luteolin in five different batches of CAROL. Notably, we found no significant difference in the content of luteolin between these batches, which proved that the composition was stable between batches. The novel structure MIPs are suitable for the specific recognition of template molecules in aqueous solution. Therefore, this study provides a technical reference for the special identification and determination of trace components in complex samples, while the novel MOF@MIP nanocomposite can also provide valuable references for the extraction and purification methods of specific substances in traditional Chinese medicine and expand the application environment of MIPs material.
研究领域:
中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yujun Zhong,Zhiyang Li,Anlin Zhang,Yuqing Peng,Hao Zhou,Yaping Guo,Danqing Lu,Lianwu Xie,Shuyun Shi
A novel electrochemical sensor MIP/AuNPs/GCE was developed by depositing gold nanoparticles and electropolymerizing molecularly imprinted film on the surface of glassy carbon electrode (GCE) for highly sensitive detection of neutral phosmet residues in fruits and vegetables. Scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the imprinted sensor. The results showed that the imprinted film on GCE was evenly prepared with better specific recognition of phosmet on MIP/AuNPs/GCE than that on bare GCE. Several parameters including deposition time, functional monomer and its molar ratio, scanning cycles, and elution time were optimized in detail. The linear range of the MIP/AuNPs/GCE prepared in this work was 0.020–4.0 nmol/L with limit of detection of 0.010 nmol/L, which was much lower than MIP/GCE (0.17 nmol/L). The identification mechanism of MIP/AuNPs/GCE for phosmet was also confirmed as adsorption-diffusion model with effective surface area increasing after mediated by AuNPs. Selectivity, reproducibility and repeatability of the MIP/AuNPs/GCE were acceptable. The recovery of applying the sensor to detect PM in real samples ranged from 91.40 % to 107.3 %, which agreed well with those of HPLC. The method of developing MIP/AuNPs/GCE sensor herein could be extended to detecting other neutral pesticide residues in food and environment.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 12
·1-7
·
IF 5.479
Li Shuhuai,Ma Xionghui,Pang Chaohai,Tian Hai,Xu Zhi,Yang Yan,Lv Daizhu,Ge Huilin
A fluorometric assay is described for the determination of Cd(II) in environmental and agricultural samples. It is making use of a molecularly imprinted polymer (MIP) and aptamer as dual recognition units, while carbon quantum dots (co-doped with sulphur and nitrogen) and gold nanoparticles (SN-CQD/Au) act as the fluorophores. The aptamer-modified MIP was placed on an SN-CQD/Au-modified indium tin oxide glass electrode. Cd(II) was detected with high selectivity by the recognition sites of the aptamer in the MIP. Fluorescence, with excitation/emission peaks at 370/430 nm, is quenched by Cd(II). Response is linear in the 20 pM to 12 nM concentration range. The detection limit is 1.2 pM. The sensor is selective for Cd(II), and recoveries from spiked waters, soils and vegetables real-world samples range between 82.1 and 113.9%.
A fluorescence sensor composed of a molecularly imprinted polymer and an aptamer as a dual identification system for Cd 2+ coupled with and carbon quantum dots (co-doped with sulphur and nitrogen) and gold nanoparticles (SN-CQDs/Au) as fluorescent element that can detect Cd 2+ with high selectivity by a dual-recognition mechanism.
研究领域:
分析化学
·
环境科学
·
材料科学
·
农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Wei Li,Qi Zhang,Yijia Wang,Yanyan Ma,Zhanchen Guo,Zhen Liu
Molecularly imprinted polymers (MIPs) and aptamers, as effective mimics of antibodies, can overcome only some drawbacks of antibodies. Since they have their own advantages and disadvantages, the combination of MIPs with aptamers could be an ideal solution to produce hybrid alternatives with improved properties and desirable features. Although quite a few attempts have been made in this direction, a facile and controllable approach for the preparation of aptamer–MIP hybrids still remains lacking. Herein, we present a new approach for facile and controllable preparation of aptamer–MIP hybrids for high-specificity and high-affinity recognition toward proteins. An aptamer that can bind the glycoprotein alkaline phosphatase (ALP) with relative weak affinity and specificity was used as a ligand, and controllable oriented surface imprinting was carried out with an in-water self-polymerization system of dopamine. A thin layer of polydopamine was formed to cover the template to an appropriate thickness. After removing the template from the polymer, an aptamer–MIP hybrid with apparently improved affinity and specificity toward ALP was obtained, giving cross-reactivity of 3.2–5.6% and a dissociation constant of 1.5 nM. With this aptamer–MIP hybrid, a plasmonic immunosandwich assay (PISA) was developed. Reliable detection of ALP in human serum by the PISA was demonstrated.
研究领域:
生物材料
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Wenzhan Yu,Minjin Wu,Jiamin Xie,Zihao Huang,Xiaoping Wei,Jianping Li
A MIP-ECL sensor was developed for the dual-potential resolved simultaneous detection of RUX and LEN. • The combining dummy template imprinting with probes click-labeling enables simultaneous detection of signal-free analytes. • This strategy provides a new detection platform for therapeutic drug monitoring in combination therapy. Combination therapy is widely used in clinics to improve efficacy and reduce side effects by administering two or more drugs for personalized precise dosing. For the combination therapy of ruxolitinib (RUX) and lenalidomide (LEN) in primary myelofibrosis treatment, therapeutic drug monitoring (TDM) of both drugs in blood is required. This paper presents the development of a molecularly imprinted (MIP) electrochemiluminescence (ECL) sensor for the simultaneous detection of RUX and LEN. To address the challenge that neither RUX nor LEN generates ECL signals on electrodes, a dummy template molecule with terminal allyl groups, structurally similar to RUX, was designed to copolymerize with LEN for the preparation of the MIP film. A novel strategy based on dummy template competitive adsorption and photo-initiated click reactions was developed for simultaneous determination of RUX and LEN. It relies on the competitive adsorption between RUX and its dummy template on MIP, followed by click labeling of two ECL probes onto the dummy template and LEN, with detection of RUX and LEN achieved by recording the cathodic and anodic ECL signals, respectively. The MIP-ECL sensor exhibits a detection range of 5 to 5000 ng/mL for both RUX and LEN, with detection limits of 0.11 ng/mL and 0.49 ng/mL, respectively. The sensor was successfully applied to the analysis of human serum samples. It not only provides a novel approach for the detection of multiple targets without responding signals using MIP, but also offers a more sensitive and convenient technical platform for TDM. An MIP-ECL sensor combining dummy-template imprinting with probe click-labeling was developed for the potential-resolved simultaneous detection of ruxolitinib and lenalidomide. This strategy overcomes the challenge of simultaneously detecting signal-free analytes and amplifies detection signals, improving sensitivity with LODs of 0.11 ng/mL for ruxolitinib and 0.49 ng/mL for lenalidomide. Download: Download high-res image (193KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Mingmei Shen,Xianwen Kan
Specifically sensitive determination of dopamine (DA) from complex real samples is very important since DA plays a major role in the function of central nervous, which presents the necessary to develop an effective sensor for the assay of DA. In this work, a dual recognition strategy was developed through introducing DA-aptamer and molecularly imprinted polymer (MIP) on gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) modified electrode. The selectivity and sensitivity of the prepared sensor were investigated upon the current response of DA itself to better represent the performances of the sensor. Compared with the single recognition element (MIP or aptamer) based sensors, the prepared dual recognition sensor displayed the highest selectivity to DA, which facilitated the proposed sensor for the detection of DA in real samples. Owing to the high surface and good conductivity of the modified AuNPs and rGO, the sensor showed a wide linear range and a low limit of detection of 5.0 × 10 -8 – 1.0 × 10 -5 mol/L and 4.7 × 10 -8 mol/L for the detection of DA, respectively. The proposed strategy of aptamer and MIP based sensor for electro-active target assay may be used as a general way for the development of other dual recognition based sensors.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
神经化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Mengmeng Song,Xianjie Sheng,Genyi Ye,Zhuicheng Xu,Xinru Li,Tong Xie,Haishan Deng,An Kang
Gut microbiota-derived tryptophan (TRP) metabolites, especially the indole derivatives, function as critical modulators of intestinal immune function and integrity via modulating the aryl hydrocarbon receptor pathway. Selective enrichment of these indole metabolites in biological samples is important for quantitative determination by routine methods such as HPLC. Here, we report a molecularly imprinted polymer (MIP) for solid-phase extraction (SPE) of TRP-derived indole metabolites from faeces of mice. The MIP was synthesized by surface polymerization by using indole-3-acetic acid (IAA) and acrylamide after cross-linking by ethylene glycol dimethacrylate (EGDMA) and initialed by 2′,2-azobisisobutyronitrile (AIBN). The MIPs were then characterized by transmission electron microscope (TEM) and fourier transform infrared spectroscopy (FTIR), and the adsorption capacity, selectivity and reusability of MIPs were systematically evaluated. Results showed that IAA-MIPs showed a uniformly distributed nanoshell layer with a thin shell thickness of 26 nm . The IAA-MIPs could selectively adsorb IAA, indole-3-propionic acid (IPA), and indole-3-lactic acid (ILA) in a mixed solution that also contains TRP and tyrosine. The adsorption capacity of IAA-MIPs only slightly decreased with the increase of recycling use. As purification material for SPE, the IAA-imprinted polymers (IAA-MIPs) were successfully applied to extract IAA, IPA, and ILA from normal and colitis mice for HPLC determination. Collectively, these surface molecularly imprinted polymers could find extensive use to selective enrichment of microbiota-derived indole metabolites in biological samples.
研究领域:
微生物学
·
分析化学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ding Yang,Yuanyuan Hui,Yingying Liu,Weizhe Wang,Chao He,Aiqing Zhao,Lusha Wei,Bini Wang
Aflatoxin M 1 (AFM 1 ) is a highly carcinogenic and toxic compound that is commonly found in dairy foods such as milk. We developed an electrochemical MIP (molecularly imprinted polymer) and aptamer (Apt) based aptasensor (Apt-Au@PEIM/AFM 1 /MIP–Apt/AuNPs)/GCE) with dual-recognition signal amplification to detect AFM 1 in milk. The MIP–Apt/AuNP/GCE sensor was prepared by synthesizing an MIP film through the electropolymerization of resorcinol on gold nanoparticles-modified electrodes. The dropwise addition of our cApt-Au@PEIM signal amplification probe could enhance the current signal response and improve the sensitivity of the sensor to detect AFM 1 . The AFM 1 present in milk could prevent the probe from binding to the sensor, thereby drastically reducing the current signal. Under optimal experimental conditions, the sensor exhibits a linear range of 0.01–200 nM and a limit of detection of 0.07 nM (S/N = 3). The developed dual-recognition aptasensor would provide a novel and rapid method for specific detection of AFM 1 in milk.
研究领域:
食品安全
·
分析化学
·
电化学
·
生物传感器
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Xuran Zhang,Rongxian Ma,Zhili Li,Jing Cao,Haoqi Ma,Yeyu Wu,Xuecai Tan,Kejing Huang
The excessive use of sulfadiazine (SDZ) poses significant health risks due to toxic residues in food and ecosystems. This work introduces a novel molecularly imprinted polymer (MIP) sensor featuring a carbon dot-based covalent organic framework (CD-COF) core as a high-performance electrochemiluminescence (ECL) emitter. Upon SDZ rebinding to imprinted sites, the sensor leverages SDZ's inherent redox activity to enable complementary differential pulse voltammetry (DPV) detection alongside ECL quenching. This dual-signal output establishes an intrinsic cross-validation mechanism that inherently corrects environmental interferences. The sensor achieves ultrasensitive detection of SDZ, with impressively low detection limits of 0.28 nM (ECL) and 1.6 nM (DPV). Validation in food matrices demonstrates exceptional accuracy and reliability, yielding recoveries of 91–110%. This study not only provides a robust tool for trace SDZ monitoring but also establishes a new paradigm for CD-COF-powered MIP sensors integrating dual-mode signals for highly reliable contaminant analysis in real-world samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 134
·期 7
·
IF 1.866
Jie-Ping Fan,Dan-Dan Liao,Yan-Long Xie,Bing Zheng,Jia-Xin Yu,Ya-Hui Cao,Xue-Hong Zhang,Hai-Long Peng
A composite material (MIP@Fe 3 O 4 @GO) based on molecular imprinted polymer (MIP), superparamagnetic Fe 3 O 4 particles and graphene oxide (GO) was prepared by the chemical coprecipitation method, and used to simultaneously separate and enrich two alkaloids (evodiamine and rutaecarpine) in the extract of Evodiae fructus . The as-prepared MIP@Fe 3 O 4 @GO was characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). The adsorption kinetics, isotherms, competitive adsorption, and reusability of MIP@Fe 3 O 4 @GO were also investigated. The results revealed that MIP@Fe 3 O 4 @GO was sensitive to the magnetic field and could be easily separated using an external magnet; MIP@Fe 3 O 4 @GO showed good recognition selectivity, as well as simultaneous enrichment and separation abilities for evodiamine and rutaecarpine in the extract of E. fructus with satisfactory recoveries. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44465.
研究领域:
材料科学
·
药物分析
·
中药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）