INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
·
2019
·
IF 2.792
Yuanfeng Wu,Yuke Shen,Ye Zhu,Jothame Mupunga,Ligen Zou,Chao Liu,Shiwang Liu,Jianwei Mao
After consuming broccoli, isothiocyanates can be produced by the hydrolytic action of myrosinase from plant and/or microbiota. Using male C57BL/6 mice, the present study investigated the effects of broccoli ingestion on the myrosinase-like activity, NAD(P)H:quinone oxidoreductase 1 (NQO1) activity, diversity and composition of the gut microbiota. Compared with the control group, continuously feeding raw or hydrolysed broccoli increased the myrosinase-like activities of the colon and caecum contents, and also improved the NQO1 activity of the colon mucosa. Significant difference between the broccoli and control feeding groups were found. 16S rRNA gene sequencing indicated that broccoli ingestion profoundly affected the composition of the gut bacteria community. The correlation between the gut bacterial community composition and microbiota myrosinase-like activity was also studied. However, one type of glucosinolate, sinigrin, had no effect on these activities, indicating that broccoli component(s), other than glucosinolate, had increased the NQO1 and myrosinase-like activities.
研究领域:
营养学
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微生物学
·
分子生物学
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Nan Zhao,Wei Song,Tianshu Sun,Weiwei Wu,Yongliang Feng,Ning Ma,Suping Wang
Prenatal exposure to Benzo[ a ]pyrene (BaP) has been shown to increase the risk of adverse birth outcomes, potentially through disruption of maternal metabolic homeostasis. However, the underlying pathways and mechanisms remain unclear. To address this knowledge gap, we employed a multi-omics approach combining experimental and epidemiological investigations. In the mouse model, untargeted metabolomics profiling revealed niacinamide (NAM) metabolism as the most significantly altered pathway in both maternal serum and placenta following BaP exposure. Mechanistically, BaP exposure reduced nicotinamide adenine dinucleotide (NAD+) synthesis while increasing its catabolism through activation of poly (ADP-ribose) polymerase 1 (PARP1) in placental tissue. Subsequent transcriptomic analysis showed that BaP-induced NAD+ depletion led to sirtuin 1 (SIRT1) suppression and consequent exacerbation of the NF-κB-mediated inflammatory response. These experimental findings were substantiated in human populations through targeted metabolomic analysis of a Chinese birth cohort, where significant NAM metabolic disturbances were observed in women with high BaP exposure experiencing preterm delivery. Our study provides novel mechanistic evidence that BaP-induced dysregulation of NAM metabolism mediates adverse birth outcomes through NAD+ deficiency and subsequent inflammatory response. These findings not only identify potential therapeutic targets but also highlight the urgent need for evidence-based environmental policy interventions and nutritional supplementation strategies to protect maternal-fetal health in high-risk populations.
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 4.446
Ying Zhang,Yucui Jiang,Bin Wei,Mingming Su,Hao Liang
Enzyme is an efficient green catalyst, but its vulnerability and difficulties of separation from products limited the application in industry. Furthermore, a large number of oxidase enzymes rely on dihydronicotinamide adenine dinucleotide (NADH) or dihydronicotinamide adenine dinucleotide phosphate (NADPH) cofactors . These cofactors are too expensive to be used in industrial processes. Thus, cofactor regeneration is an important method to avoid the consumption of large quantities of oxidized cofactor NAD(P) + in enzyme-catalyzed reactions. In this paper, Cu based metal nucleotides coordination polymers (MNCPs) with NAD(P)H oxidase activity were constructed by self-assembly of copper ions and nucleotides. Cu 2+ /AMP and Cu 2+ /GMP showed high catalytic activity and high reusability. Then, Cu 2+ /AMP with NADH oxidase activity was combined with NAD + dependent alcohol dehydrogenase (ADH) to construct a bienzyme cascade system with circulating cofactor, which applied to the conversion of benzyl alcohol to benzaldehyde. In this system, ADH exhibited high activity due to the increase of substrate concentration and the inhibition of the diffusion of intermediate products. Besides, the bienzyme cascade system also showed high stability and reusability. After 8 cycles, the immobilized ADH remained more than 57% of the initial activity. The prepared bienzyme cascade system can extensively extended its application in industrial production.
研究领域:
酶工程
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催化
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生物技术
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有机化学
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 46
·26729-26733
·
IF 3.049
Ya Zhu,Jialing Han,Qian Zhang,Zhou Zhao,Jin Wang,Xiaowei Xu,Haiping Hao,Jun Zhang
Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a fluorophore and quinone propionic acid (QPA) as a recognition group. The probe, which has high selectivity for hNQO1 and a Stokes shift of about 117 nm, shows a linear relationship with hNQO1 concentrations in the range of 0.25–3 μg ml−1 with the color changes from yellow to red and was successfully applied to intracellular hNQO1 imaging.
研究领域:
生物化学
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医学诊断
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肿瘤学
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DOI
PubMed
高影响因子
Biotechnology Journal
·
2025
·Vol. 20
·期 2
·e202400707
·
IF 3.1
Fengrui Yu,Hongwen Li,Xianglong Li,Jianping Shi,Yanbin Feng,Song Xue
Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), provides a direct method for maintaining NAD levels, which may alleviate aging and metabolic disorders. However, the enzymatic conversion of NMN in cascade reactions is limited by intermediate product inhibition, and quantitative insights into these limitations remain scarce. Here, an efficient multienzyme cascade system was developed by quantifying intermediate inhibition, which synthesizes NMN from D-ribose in three tandem reactions with an Adenosine Triphosphate (ATP) regeneration system and pyrophosphatase (PPase). A critical Adenosine Diphosphate (ADP) concentration of 0.5 mM was determined, which inhibits phosphoribosyl pyrophosphate synthetase (Prs) at 0.08 µM. The incorporation of an ATP regeneration system and PPase markedly increased the NMN yield to 81.3%. The intermediate phosphoribosyl pyrophosphate (PRPP) hydrolysis rate was measured at 3 µM/min. The highly active nicotinamide phosphoribosyltransferase (Nampt) could compete with PRPP hydrolysis, thereby increasing the yield of NMN. This research facilitates large-scale, efficient NMN manufacturing.
研究领域:
生物化学
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代谢工程
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合成生物学
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DOI
PubMed
高影响因子
Theranostics
·
2024
·
IF 12.4
Yitian Shi,Jiayin Peng,Mengfan Liu,Xiling Qi,Siyu Li,Qiangqiang Li,Qing Jiang,Liming Zheng,Jiankun Xu,Yun Zhao,Yifeng Zhang
The process of skeletal regeneration initiated by stem cells following injury, especially in fractures, is significantly impaired by aging and adverse factors. Nicotinamide mononucleotide (NMN), a critical endogenous precursor of nicotinamide adenine dinucleotide (NAD), has garnered extensive attention for its multifaceted regulatory functions in living organisms and its wide-ranging therapeutic potential. However, whether NMN contributes to trauma-induced skeletal regeneration remains unclear. Methods : The transverse femoral shaft fracture model was employed to evaluate the potential advantages of NMN administration for overall repair during the initial fracture stages in male mice through micro-CT analysis, histochemistry, and biomechanical testing. The pro-proliferative function of NMN on skeletal stem cells (SSCs) was investigated through flow cytometry, qRT-PCR, NAD content measurement, and cell proliferation assay. Results : In this study, we observed that the administration of NMN during the initial phase of fracture in mice led to a larger callus and corresponding improvement in micro-CT parameters. NMN enhances the cartilaginous component of the callus by elevating the NAD content, consequently accelerating subsequent endochondral ossification and the fracture healing process. Subsequent analyses elucidated that NMN was beneficial in promoting the expansion of diverse stem cells in vivo and in vitro potentially via modulation of the Notch signaling pathway. Moreover, the depletion of macrophages profoundly obstructs the proliferation of SSCs. Conclusion : Our discoveries provide a potential strategy for enhancing fracture healing through stimulation of callus SSC proliferation at an early stage, shedding light on the translational value of NMN as an enhancer for skeletal regeneration and highlighting the pivotal role of macrophage-stem cell interactions in governing the regenerative influence of NMN on stem cells.
研究领域:
再生医学
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干细胞生物学
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骨科
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分子生物学
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DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Yanpeng Lin,Hangtian Wu,Jun Wang,Wanling He,Jiahui Hou,Vidmi Taolam Martin,Chencheng Zhu,Yupeng Chen,Junyuan Zhong,Bin Yu,Aiping Lu,Daogang Guan,Genggeng Qin,Weiguo Chen
Chondrocyte senescence, synovitis, and decreased level of lubrication play pivotal roles in the pathogenesis of age-related osteoarthritis (AROA). However, there are currently no effective therapeutic interventions capable of altering the progression of OA until it reaches advanced stages, necessitating joint replacement. In this study, lubricious and drug-loaded hydrogel microspheres were designed and fabricated by utilizing microfluidic technology for radical polymerization of chondroitin sulfate methacrylate and incorporating nicotinamide adenine dinucleotide (NAD)-loaded liposomes modified with lactoferrin that are positively charged. Mechanical, tribological, and drug release analyses demonstrated enhanced lubrication properties and an extended drug dissemination time for the NAD@NPs@HM microspheres. In vitro assays unveiled the ability of NAD@NPs@HM to counteract chondrocyte senescence. RNA sequencing analysis, untargeted metabolomics analysis, and in vitro experiments on macrophages revealed that NAD@NPs@HM can regulate the metabolic reprogramming of synovial macrophages, promoting their repolarization from the M1 to M2 phenotype, thereby alleviating synovitis. Intra-articular injection of NAD@NPs@HM in aged mice reduced the mechanisms associated with AROA. These results suggest that NAD@NPs@HM may provide extended drug release, improved joint lubrication leading to better gait, and attenuation of AROA pathogenic processes, indicating its potential as a therapeutic approach for AROA.
研究领域:
生物材料学
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医学
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分子生物学
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DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2026
·
IF 4.5
Hao Wu,Junfeng Luo,Zonghui Jian,Min Yang,Jingying Zhao,Jing Fu,Shixiong Yan,Tengfei Dou,Junjing Jia,Lixian Liu,Zhiqiang Xu
This study aimed to compare the pectoris muscle chemical composition, NAD⁺-related metabolic regulation, and lipidomic profiles of Daweishan miniature chickens (M3) and Arbor Acre broilers (A3) to characterize breed-dependent metabolic differences. For this, 100 one-day hatched chicks were randomized into two groups (A3 and M3; n=50/breed/group) by following a completely randomized design and subjected to a 90-day feeding trial under identical conditions. At the 90 th day, six birds from each group (containing an equal number of males and females) were slaughtered, and the pectoralis muscle was sampled for subsequent analysis. The chemical composition analysis showed that the M3 group had higher crude protein and ash contents, whereas lower moisture and crude fat contents compared to the A3 group (p < 0.05). Similarly, NAD⁺ and its intermediates (nicotinamide, nicotinamide mononucleotide, and nicotinamide riboside) concentrations were also higher in the M3 group compared to the A3 group (p < 0.05). In addition, the enzymatic activities of SIRT1, LKB1, and AMPK were also greater in the M3 group than the A3 group (p < 0.05). Lipidomic profiling analysis showed distinct separation between groups, with triglyceride species downregulation, and fatty acids were upregulated in the M3 group. The KEGG pathway enrichment analysis showed that there was a significant difference in glycerolipid metabolism, fatty acid metabolism, and glycerophospholipid pathways across the groups. Correlation analysis revealed a strong association between NAD⁺ metabolites, regulatory enzymes, and differential lipid species. Based on results, the Daweishan miniature chickens maintains a metabolic phenotype characterized by enhanced NAD⁺ metabolism, greater enzymatic regulation, and reduced triglyceride accumulation, while Arbor Acre broilers exhibit a lipid deposition-oriented profile.
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高影响因子
ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 42
·期 12
·1991-2003
·
IF 6.15
Dong Wen-li,Zhong Jia-hong,Chen Yun-qing,Xie Jin-feng,Qin Yun-yun,Xu Jiang-ping,Cai Ning-bo,Li Meng-fan,Liu Lu,Wang Hai-tao
We have previously shown that roflupram (ROF) protects against MPP + -induced neuronal damage in models of Parkinson’s disease (PD). Since impaired degradation of α-synuclein (α-syn) is one of the key factors that lead to PD, here we investigated whether and how ROF affects the degradation of α-syn in rotenone (ROT)-induced PD models in vivo and in vitro. We showed that pretreatment with ROF (10 μM) significantly attenuated cell apoptosis and reduced the level of α-syn in ROT-treated SH-SY5Y cells. Furthermore, ROF significantly enhanced the lysosomal function, as evidenced by the increased levels of mature cathepsin D (CTSD) and lysosomal-associated membrane protein 1 (LAMP1) through increasing NAD + /NADH and the expression of sirtuin 1 (SIRT1). Pretreatment with an SIRT1 inhibitor selisistat (SELI, 10 μM) attenuated the neuroprotection of ROF, ROF-reduced expression of α-syn, and ROF-increased expression levels of LAMP1 and mature CTSD. Moreover, inhibition of CTSD by pepstatin A (20 μM) attenuated ROF-reduced expression of α-syn. In vivo study was conducted in mice exposed to ROT (10 mg·kg −1 ·d −1 , i.g.) for 6 weeks; then, ROT-treated mice received ROF (0.5, 1, or 2 mg·kg −1 ·d −1 ; i.g.) for four weeks. ROF significantly ameliorated motor deficits, which was accompanied by increased expression levels of tyrosine hydroxylase, SIRT1, mature CTSD, and LAMP1, and a reduced level of α-syn in the substantia nigra pars compacta. Taken together, these results demonstrate that ROF exerts a neuroprotective action and reduces the α-syn level in PD models. The mechanisms underlying ROF neuroprotective effects appear to be associated with NAD + /SIRT1-dependent activation of lysosomal function.
研究领域:
神经科学
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2025
·
IF 3.9
Bao-Di Ma,Jia-Yi Li,Jian-He Xu,Tao Yu,Xu-Dong Kong
Methanol dehydrogenase (MDH), a representative of Type III alcohol dehydrogenases (ADHs), plays a pivotal role in methanol assimilation pathways, making it a key enzyme for the biosynthesis of chemicals and fuels from one-carbon feedstocks. An activator protein belonging to the Nudix hydrolase family, ACT, was found to increase the activity of MDH by 40-fold. Despite the widespread observation of this in vitro activation phenomenon in pairs of type III alcohol dehydrogenases and Nudix hydrolases, the mechanistic details have remained unresolved for decades. Here, we uncover a regulation mechanism in which MDH activation arises from the hydrolytic removal of ADP-ribose (ADPR), a potent inhibitor derived from NAD + degradation, by the ADPRase activity of ACT. This discovery challenges the previously proposed 'activation' models, revealing that ACT-mediated ADPR clearance disinhibits MDH rather than directly enhancing catalysis. By combining crystallographic analysis, kinetics, and inhibition assays, we demonstrate that ADPR inhibits MDHs with submicromolar K i values, highlighting its potential regulatory role in metabolic networks. Our findings redefine the widespread ‘activation’ of type III ADHs, providing valuable insights into alcohol metabolism and new directions for engineering synthetic methanol utilization pathways.
研究领域:
生物化学
·
结构生物学
·
酶学
·
代谢工程
·
微生物学
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DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 14
·7507-7519
·
IF 6.317
Meng Ru,Wanwan Wang,Zhenya Zhai,Ruxia Wang,Yumeng Li,Jiang Liang,Damini Kothari,Kaimin Niu,Xin Wu
The nicotinamide adenine dinucleotide (NAD+) level shows a temporal decrease during the aging process, which has been deemed as an aging hallmark. Nicotinamide mononucleotide (NMN), a key NAD+ precursor, shows the potential to retard the age-associated functional decline in organs. In the current study, to explore whether NMN has an impact on the intestine during the aging process, the effects of NMN supplementation on the intestinal morphology, microbiota, and NAD+ content, as well as its anti-inflammatory, anti-oxidative and barrier functions were investigated in aging mice and D-galactose (D-gal) induced senescent IPEC-J2 cells. The results showed that 4 months of NMN administration had little impact on the colonic microbiota and NAD+ content in aging mice, while it significantly increased the jejunal NAD+ content and improved the jejunal structure including increasing the villus length and shortening the crypt. Moreover, NMN supplementation significantly up-regulated the mRNA expression of SIRT3, SIRT6, nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), the catalytic subunit of glutamate-cysteine ligase (GCLC), superoxide dismutase 2 (SOD2), occludin, and claudin-1, but down-regulated the mRNA expression of tumor necrosis factor alpha (TNF-α). Specifically, in the D-gal induced senescent IPEC-J2 cells, 500 μM NMN restored the increased mRNA expression of interleukin 6 (IL6ST), IL-1A, nuclear factor (NF-κB1), and claudin-1 to normal levels to some extent. Furthermore, NMN treatment significantly affected the mRNA expression of antioxidant enzymes including NQO1, GCLC, SOD 2 and 3, and GSH-PX1, 3 and 4. In addition, 200 μM NMN enhanced the cell viability and total antioxidant capacity and lowered the reactive oxygen species level of senescent IPEC-J2 cells. Notably, NMN restored the down-regulated protein expression of occludin and claudin-1 induced by D-gal. The above data demonstrated the potential of NMN in ameliorating the structural and functional decline in the intestine during aging.
研究领域:
老年学
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分子生物学
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
2D Materials
·
2023
·
IF 5.5
Qiang Peng,Nian Jiang,Ling Qian,Wanqing Yue
Oxidation-derived nanoparticles (CDs/TiOx) of Ti3C2 were prepared for the first time by simple oxidation of Ti3C2. CDs/TiOx was a two-dimensional hybrid of amorphous carbon and titanium oxides with a lateral dimension of about 50 nm. H2O2 was used as the oxygenator and the reduction product was H2O, which was environmentally friendly and inexpensive. Carboxyl, carbonyl, and hydroxyl groups are formed naturally during the oxidation derivative process. The decreased size increases the specific surface area and provides the possibility for the abundant presence of functional groups. The oxidation process converts MXene from reducing to oxidizing and achieves the ability of the derivatives to mimic peroxidase. Compared with natural horseradish peroxidase, the Michaelis constant for H2O2 was 10-fold lower. A cascade catalytic reaction system of glucose oxidase with CDs/TiOx was constructed, and the generated H2O2 could be further used to catalyze the oxidation of NADH to NAD+. With the assistance of NAD+-dependent dehydrogenase, NADH could be restored to 95% of the initial level. This assay system can detect glucose levels scientifically and accurately in the range of 0.02-10 mM and remains viable after 20 cycles.
研究领域:
材料科学
·
生物化学
·
纳米技术
·
酶工程
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）