JOURNAL OF CLINICAL MICROBIOLOGY
·
2024
·Vol. 62
·期 2
·
IF 6.1
Zhimin Lu,Xiaonan Wang,Licai Ma,Leina Dou,Xiangjun Zhao,Jin Tao,Yang Wang,Shaolin Wang,Dejun Liu,Yingbo Shen,Xuezhi Yu,Wenbo Yu,Liangxi Jia,Zhanhui Wang,Jianzhong Shen,Kai Wen
Rapid phenotypic detection assays, including Carba NP and its variants, are widely applied for clinical diagnosis of carbapenemase-producing Enterobacterales (CPE). However, these tests are based on the acidification of the pH indicator during carbapenem hydrolysis, which limits test sensitivity and speed, especially for the detection of CPE producing low-activity carbapenem (e.g., OXA-48 variants). Herein, we developed a novel rapid and sensitive CPE detection method (Carba PBP) that could measure substrate (meropenem) consumption based on penicillin-binding protein (PBP). Meropenem-specific PBP was used to develop a competitive lateral flow assay (LFA) for meropenem identification. For the detection of carbapenemase activity, meropenem concentration was optimized using a checkerboard assay. The performance of Carba PBP was evaluated and compared with that of Carba NP using a panel of 94 clinical strains characterized by whole-genome sequencing and carbapenem susceptibility test. The limit of detection of PBP-based LFA for meropenem identification was 7 ng mL−1. Using 10 ng mL−1 meropenem as the substrate, Carba PBP and Carba NP could detect 10 ng mL−1 carbapenemase within 25 min and 1,280 ng mL−1 CPE in 2 h, respectively. The sensitivity and specificity were 100% (75/75) and 100% (19/19) for Carba PBP and 85.3% (64/75) and 100% (19/19) for Carba NP, respectively. When compared with Carba NP, Carba PBP showed superior performance in detecting all the tested CPE strains (including OXA-48-like variants) within 25 min and presented two orders of magnitude higher analytical sensitivity, demonstrating potential for clinical diagnosis of CPE.
研究领域:
微生物学
·
临床诊断
·
分子生物学
·
传染病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 17
·175501
·
IF 3.874
Jiamin Gao,Lu Huang,Zhuomin Zhang,Gongke Li
It is of great significance to construct specially designed gold nanocrystals (AuNCs) with precisely controllable size and morphology to achieve an excellent physicochemical performance. In this work, sea urchin-shaped AuNCs with tunable plasmonic property were successfully synthesized by the hybridized double-strand poly adenine (dsPolyA) DNA-directed self-assembly technique. Hybridized dsPolyA as the directing template had suitable rigidity and upright conformation, which benefited the controllable formation of these anisotropic multi-branched AuNCs with the assistance of surfactant. The effects of essential conditions influencing the synthesis and precise morphology control were investigated in detail. COMSOL simulation was used to evaluate their electromagnetic field distribution according to their morphologies, and the result suggested that sea urchin-shaped AuNCs had abundant 'hot spots' for surface-enhanced Raman scattering (SERS) detection due to their regular nanoprotuberance structure. Finally, sea urchin-shaped AuNCs with excellent SERS and catalytic performance were applied for the quantitative analysis of food colorant and catalytic degradation of potential pollutants. The SERS enhancement factor of sea urchin-shaped AuNCs was up to 5.27 × 106, and the catalytic degradation rate for 4-NP by these AuNCs was up to −0.13min−1.
研究领域:
纳米技术
·
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Longxue Yu,Yao Zhou,Xin Wang,Lingyun Xue,Shuo Wang,Yi Liu,Xuezhen Gao,Xunyong Liu
Cysteine (Cys) is essential in human physiological processes, and its abnormal levels are linked to various diseases, necessitating real-time monitoring in biological samples. Conventional detection methods, however, are often limited by the operational complexity and susceptibility to interference. To overcome these issues, we developed a chitosan–gold nanoparticle (CS–Au NP) gel sensor for dual-mode visual detection of Cys via gel–sol transition and colorimetry. This approach significantly reduces false positives that are common in single-signal methods. The sensor utilizes chitosan as an in situ reductant to form Au NPs, which together with chitosan amine groups and hydrogen bonding construct a 3D gel network. In the presence of Cys, its thiol groups competitively bind to Au NPs, disrupting cross-linking and triggering a gel–sol transition. Quantitative analysis based on the coverage ratio (Cr) on paper substrates showed high sensitivity with a detection limit of 2.08 μM and strong linearity (R2 = 0.9981). Concurrently, Au NPs released from the gel assemble into nanoaggregates, causing a red-to-blue-gray color shift due to SPR changes. This allows both visual qualitative assessment and smartphone-based RGB quantification, achieving a linear range of 20–1000 μM and an LOD of 2.23 μM. The dual-mode design ensures high selectivity, with minimal response to 19 interfering amino acids. Practical tests in human urine and bovine serum yielded recoveries of 100.24%–125.80%, confirming accuracy in complex matrices. Integrated with smartphone imaging, this portable system is ideal for point-of-care testing, offering a reliable and cost-effective platform for Cys monitoring and precision medicine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Yao Chen,Zhanghao Huang,Cheng Ji,Jia-Hai Shi
Background Biomaterials can improve cardiac repair combined with transplantation of bone marrow mononuclear cells (BMMNCs). In this study, we compared the phenotype and cardiac repair between human heart valve-derived scaffold (hHVS) and natural protein/polycaprolactone (NP/PCL) anchored BMNNCs. Methods and results BMMNCs were obtained from mice five days following myocardial infarction. Subsequently, BMMNCs were separately cultured on hHVS and PCL. Proliferation and cardiomyogenic differentiation were detected in vitro. Cardiac function was measured after transplantation of cell-seeded cardiac patch on MI mice. After that, the BMMNCs were collected for mRNA sequencing after culturing on the scaffolds. Upon anchoring onto hHVS or PCL, BMMNCs exhibited an increased capacity for proliferation in vitro, however, the cells on hHVS exhibited superior cardiomyogenic differentiation ability. Moreover, both BMMNCs-seeded biomaterials effectively improved cardiac function after 4 weeks of transplantation, with reduced infarction area and restricted LV remodeling. Cell-seeded hHVS was superior to cell-seeded PCL. Conclusion BMMNCs on hHVS showed better capacity in both cell cardiac repairing and improvement for cardiac function than on PCL. Compared with seeded onto PCL, BMMNCs on hHVS had 253 genes up regulated and 189 genes down regulated. The reason for hHVS’ better performance than PCL as a scaffold for BMMNCs might be due to the fact that optimized method of decellularization let more cytokines in ECM retained.
研究领域:
心血管医学
·
再生医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 21
·18059-18066
·
IF 2.324
Li Zhen,Yu Libo,Song Hai,Feng Lei,Wang Xingpeng
TiO 2 nanoparticles (NP) at top of TiO 2 nanotube (TiO 2 NP@TiO 2 NT) double-layered architecture is constructed to combine the advantages of TiO 2 NP and TiO 2 NT together. This composite TiO 2 NP@TiO 2 NT architecture as photoanode possesses a larger surface area for more QDs loading, and highly tubular structure for electron swift transport. Based on this architecture, CdSe/CdS quantum dots (QDs) have been successfully synthesized by successive ionic layer adsorption reaction (SILAR) method for quantum dots-sensitized solar cell application. The photovoltaic performance of QDSSCs based on TiO 2 NP@TiO 2 NT have been investigated in contrast with bare TiO 2 NP and bare TiO 2 NT architectures with almost the same thickness. The results show that the power conversion efficiency (PCE) of QDSSCs could be enhanced using TiO 2 NP@TiO 2 NT and improved to 3.26%, which is 80% and 38% higher than QDSSCs based on bare TiO 2 NT and bare TiO 2 NP, respectively. The BET surface area, UV–vis absorption spectra, and incident photon to current conversion efficiency (IPCE) measurements results show the evidence that the TiO 2 NP@TiO 2 NT can combine advantages of TiO 2 NP and TiO 2 NT structures together and lead to a higher light harvesting efficiency, electron collecting efficiency, and efficient electron transport.
研究领域:
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yu-Shi Shen,Xiao-Hui Yao,Chen-Xi He,Run-Ze Hu,Jia-Xin Yang,Dong-Yang Zhang,Tao Chen
Modern bioreactors primarily focus on fixing enzymes on materials to form fixed bed reactors, but the reaction efficiency is unstable. More importantly, the enzymes easily fall off or even inactivate, and the fluid risks leaking due to excessive pressure during flow. In this study, a directional channel continuous microfluidic reactor with laccase covalently immobilized in the internal channel is made from natural wood. After dimethylacetamide/lithium chloride (DMAc/LiCl) treatment of delignified wood, the micropores (ray cells, pits and nanopores) in directional channel walls are fully exposed. When the fluid passes through, the turbulence near the pits increases, resulting in good mass and heat transfer, which enhances the catalytic efficiency of laccase . The reactor has good stability and maintains more than 80% substrate conversion in the pH range of 3–7 and the temperature range of 15–55 ℃. In the degradation of 4-nitrophenol (4-NP), a 94.42% degradation rate is achieved in only 30 min, and 86.93% efficiency is maintained after 25 cycles of catalysis, indicating its excellent reusability. The directional reactor with porous inner walls is simple to prepare and easy to scale up, which provides excellent convenience for continuous industrial production and shows great commercial application potential.
研究领域:
生物工程
·
环境科学
·
酶技术
✓ 已验证
产品关联
DOI
高影响因子
ACS ES&T Water
·
2022
·
IF 0
Xiaofeng Fang,Ruo Li,Ziyi Zhang,Xingran Zhang,Fang Li,Yanbiao Liu
Excellent perm-selectivity and fouling resistance are highly desirable for membrane separation in water treatment. Electrically conductive membranes have been recognized as an effective means for overcoming the permeability–selectivity trade off and enhancing the antifouling capability. However, the facile and controllable fabrication of conductive membranes with good flexibility, high conductivity, and stable separation performance remains a challenge. Herein, a novel conductive poly(vinylidene fluoride) (PVDF) ultrafiltration (UF) membrane with a thin silver nanoparticle (Ag NP) coating layer was developed via nonsolvent phase separation and green in situ reduction methods. Especially, the tannic acid/Fe3+ (TA/Fe) complex and carbon nanotube (CNT) were first blended into a PVDF UF membrane. The thin Ag NP coating layer was then in situ formed and firmly fixed on the membrane with TA to produce a conductive PVDF/TA-Ag composite membrane. The introduced Ag NP coating layer not only narrowed the pore size but also increased the electrical conductivity of the PVDF/TA membrane, which resulted in an enhanced electrostatic repulsion and remarkable humic acid (HA) rejection. The optimal membrane (i.e., PVDF/TA-Ag12) achieved an improved HA solution flux of 265 LMH/bar and an HA rejection of 97% under −2 V applied voltage, which are 2 times and 1.7 times higher than those of the uncharged membrane, respectively. Moreover, the PVDF/TA-Ag12 membrane exhibited a superior antifouling performance under an external electrical field. Meanwhile, the electrochemical reduction of Ag+ to Ag0 on the membrane matrix can effectually avoid the Ag NP leaching, keeping the stability of the separation performance. These findings provide an alternative and cost-effective method for the development of polymer conductive membranes to enhance pollutant rejection and mitigate membrane fouling.
研究领域:
材料科学
·
环境工程
·
水处理技术
✓ 已验证
产品关联
DOI
Advanced Healthcare Materials
·
2024
·
IF 10
Zhiming Hou,Xinyu Ren,Zhuangzhuang Sun,Ruoqi An,Mingzhi Huang,Cen Gao,Mengying Yin,Guangxiu Liu,Dengqi He,Hongliang Du,Rongbing Tang
Bacteria-derived hydrogen sulfide (H 2 S) often contributes to the emergence of antibiotic-recalcitrant bacteria, especially persister (a sub-population of dormant bacteria), thus causing the treatment failure of Catheter-associated urinary tract infection (CAUTI). Here, an H 2 S harvester nanosystem to prevent the generation of persister bacteria and disrupt the dense biofilm matrix by the self-adaptive ability of shape-morphing is prepared. The nanosystem possesses a core-shell structure that is composed of liquid metal nanoparticle (LM NP), AgNPs, and immobilized urease. The nanosystem decomposes urea contained in urine to generate ammonia for eliminating bacteria-derived H 2 S. Depending on the oxidative layer of liquid metal, the nanosystem also constitutes a long-lasting reservoir for temporarily storing bacteria-derived H 2 S, when urease transiently overloads or in the absence of urine in a catheter. Depriving H 2 S can prevent the emergence of persistent bacteria, enhancing the bacteria-killing efficiency of Ga 3+ and Ag + ions. Even when the biofilm has formed, the urine flow provides heat to trigger shape morphing of the LM NP, tearing the biofilm matrix. Collectively, this strategy can turn trash (urea) into treasure (H 2 S scavengers and biofilm rippers), and provides a new direction for the antibacterial materials application in the medical field.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
微生物学
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
CELLULAR & MOLECULAR BIOLOGY LETTERS
·
2023
·Vol. 28
·期 1
·1-24
·
IF 8.3
Lei Ming,Lin Hui,Shi Deyao,Hong Pan,Song Hui,Herman Bomansaan,Liao Zhiwei,Yang Cao
Background Intervertebral disc degeneration (IVDD) is the major cause of low-back pain. Histone deacetylase 9 (HDAC9) was dramatically decreased in the degenerative nucleus pulposus (NP) samples of patients with intervertebral disc degeneration (IVDD) according to bioinformatics analysis of Gene Expression Omnibus (GEO) GSE56081 dataset. This study aims to investigate the role of HDAC9 in IVDD progression.Methods The contribution of HDAC9 to the progression of IVDD was assessed using HDAC9 knockout (HDAC9KO) mice and NP-targeted HDAC9-overexpressing mice by IVD injection of adenovirus-mediated HDAC9 under a Col2a1 promoter. Magnetic resonance imaging (MRI) and histological analysis were used to examine the degeneration of IVD. NP cells were isolated from mice to investigate the effects of HDAC9 on apoptosis and viability. mRNA-seq and coimmunoprecipitation/mass spectrometry (co-IP/MS) analysis were used to analyze the HDAC9-regulated factors in the primary cultured NP cells.Results HDAC9 was statistically decreased in the NP tissues in aged mice. HDAC9KO mice spontaneously developed age-related IVDD compared with wild-type (HDAC9WT) mice. In addition, overexpression of HDAC9 in NP cells alleviated IVDD symptoms in a surgically-induced IVDD mouse model. In an in vitro assay, knockdown of HDAC9 inhibited cell viability and promoted cell apoptosis of NP cells, and HDAC9 overexpression had the opposite effects in NP cells isolated from HDAC9KO mice. Results of mRNA-seq and co-IP/MS analysis revealed the possible proteins and signaling pathways regulated by HDAC9 in NP cells. RUNX family transcription factor 3 (RUNX3) was screened out for further study, and RUNX3 was found to be deacetylated and stabilized by HDAC9. Knockdown of RUNX3 restored the effects of HDAC9 silencing on NP cells by inhibiting apoptosis and increasing viability.Conclusion Our results suggest that HDAC9 plays an important role in the development and progression of IVDD. It might be required to protect NP cells against the loss of cell viability and apoptosis by inhibiting RUNX3 acetylation and expression during IVDD. Together, our findings suggest that HDAC9 may be a potential therapeutic target in IVDD.Graphical Abstract
研究领域:
骨科
·
分子生物学
·
治疗学
·
生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shimin Chen,Junnan Han,Zhihuan Li,Zijie Lin,Min Wang,Hucheng Song,Xiaodong Pi,Dongke Li,Linwei Yu,Jun Xu,Kunji Chen
Lithium-carbon dioxide (Li-CO 2 ) batteries using high ion-conductive inorganic molten salt electrolytes, as a kind of promising carbon–neutral technology, have attracted much attention due to the high safety and high energy density. However, sluggish CO 2 reduction reaction (CO 2 RR) and evolution reaction (CO 2 ER) kinetics at cathodes make these batteries losing most of their capacity and power. Here, by engineering the carbon-free three-dimensional (3D) Cr-N-Ru catalysts on silicon nanopillars (Si NP) cathodes, we report a reversible solar photothermal (SPT) molten salt Li-CO 2 battery where solar energy can be efficiently harvested (over 90 % absorption rate from 300 nm to 2500 nm), concentrated and converted to heat. Theoretical simulations support that the 3D Cr-N-Ru catalysts exhibit lower energy barrier and more effectively catalyze the CO 2 RR and CO 2 ER process compared to individual Ru or CrN catalyst. The SPT Li-CO 2 battery shows a reversible cycle lifespan of 83 cycles with lower charge potential (3.2 V) and higher energy efficiency of 72 %, corresponding to ∼ 0.9 V potential gap, than the one using conventional Ru catalysts assembled on Si NP cathode. This battery technology paves the way for developing the next-generation safe and high-energy–density energy storage batteries with carbon–neutral effect.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2024
·
IF 5.7
Yining Chang,Ran He,Runqiu Wang,Yanli Wei,Li Wang
Nitrophenols are manufactured chemicals used in making industrial products and released from auto exhaust. Once nitrophenols enter the soil, the phytotoxicity causes forest decline. Exposure to nitrophenols will result in adverse health effects, such as cataracts, cyanosis and potential carcinogenicity. Based on structural matching, this study describes a sensing system of 2-hydroxylpropyl-β-cyclodextrin (HP-β-CD) modified sulphur quantum dots (HP-SQDs) for the selective detection of para-nitrophenol (p-NP). HP-SQDs are synthesized by the assembly fission method. The cyclodextrin-based molecular cages detected p-NP selectively by fluorescence quenching, which resulted from the structural matching and photoinduced electron transfer (PET) between p-NP and HP-β-CD. The inclusion of nitrophenols in cyclodextrin cages causes the decreased fluorescence intensity of HP-SQDs, with the detection limit of 0.25 μmol L−1 for p-NP. The theoretical calculation of the binding energy and inclusion constant between HP-β-CD and nitrophenols reveals the high selectivity of p-NP over other isomers by HP-SQDs. This fluorescent cage sensing approach provides a new idea for the identification of isomers.
研究领域:
环境化学
·
分析化学
·
材料科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2021
·
IF 7.711
Zongbao Sun,Yunlong Gao,Zeng Niu,Haodong Pan,Xuechao Xu,Wen Zhang,Xiaobo Zou
Paper-based fluorescence devices, with smartphone aids, bring considerable operation convenience for tetracycline (TC) sensing. Nevertheless, they must meet the challenge in real determination against complicated backgrounds. Considering that, we present a programmable-printing paper-based device and then apply it to TC determination for various natural samples. MoS2 NPs and Gmp/Eu-Cit are synthetized as composite probes. A static quenching process is found with MoS2 NP fluorescence at 430 nm, while significant magnification of Gmp/Eu-Cit emission is obtained at 617 nm, establishing a valuable ratiometric indicator. Remarkably, two-stage programmable printing maximizes the proposed sensing capability. A transitive device, containing a gradually changing amount of a certain probe, is prepared to sense TC. With a homemade smartphone application and 3D-printed measurement chamber, the corresponding signals are examined to explore optimal setups. These setups are automatically processed to prepare the final-version device, not requiring manual operations. Benefitting from this interesting feature, the proposed device gains many rewards in performances. It effectively senses TC in a wide range from 12.7 nM to 80 μM and simultaneously provides naked eye-legible signals and smartphone-based readouts with confident selectivity and stability. This device is consequently applied for various samples of soil, river water, milk, and serum and meets well with HPLC–MS and recovery tests.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）