TALANTA
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2026
·
IF 6.7
Donghui Wang,Jiawei Feng,Ningning Li,Yuanyuan Liu,Qingqing Lu,Yang Li,Yiming Zhang
Detection technologies based on molecularly imprinted polymers (MIPs) have been developed for analyzing ochratoxin A (OTA), a highly toxic food contaminant. However, conventional MIPs suffer from disordered imprinting cavities, which compromise their analytical performance for OTA. Herein, we first proposed a luminescent metal organic framework (LMOF)-based paper-imprinted sensor (FP@Eu-DCBA@MIP) via a dummy template-derivatization-based oriented surface imprinting strategy for efficient OTA analysis. By incorporating a cis-dihydroxy-derived dummy template (OTA-CD) during imprinting, FP@Eu-DCBA@MIP achieved ordered recognition cavities. Compared to the non-imprinted material (FP@Eu-DCBA@NIP), FP@Eu-DCBA@MIP exhibited better adsorption capacity (58.82 mg g -1 ) and selectivity (imprinting factor: 3.6) for OTA. The LMOF (Eu-DCBA) served as the detection unit, endowing FP@Eu-DCBA@MIP with good sensitivity for OTA (limit of detection: 0.00352 mg L -1 ) through the antenna effect between Eu-DCBA and OTA. The sensor was also successfully applied to OTA analysis in food samples, demonstrating the potential of FP@Eu-DCBA@MIP for OTA detection in complex matrices.
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 6
·407
·
IF 5.4
Fan Lihua,Ou Zongyuan,Li Fan,Shi Yiheng,Wu Di,Wu Yongning,Li Guoliang
Sensitive and reliable quantification of Ochratoxin A (OTA) in food is essential for ensuring food safety and protecting public health. In this study, a dual-mode colorimetric and Surface-Enhanced Raman Spectroscopy (SERS) biosensor was developed by integrating DNAzyme-responsive hydrogel and Au@Pt@4-MBN nanozyme for signal amplification. Upon introduction of OTA, a specific cascade reaction was triggered, activating the generated DNAzymes and leading to the disintegration of the hydrogel network and the subsequent release of encapsulated nanozymes. The extent of hydrogel disintegration exhibited a positive correlation with OTA concentration, enabling straightforward quantitative detection. The biosensor demonstrated excellent sensitivity, with detection limits of 0.0668 ng mL⁻¹ (colorimetric) and 0.0342 ng mL⁻¹(SERS). When applied to spiked corn samples, it achieved high recoveries of 98.70%-100.93% (colorimetric) and 95.6%-104.27% (SERS), confirming its reliability. The method also demonstrated high practical applicability for authentic non-spiked samples, with a positive detection rate of 10% that was consistent with the results of the national standard method. This robust platform offers significant potential for sensitive mycotoxin monitoring in food and environmental safety. Graphical The alternative text for this image may have been generated using AI.
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高影响因子
TALANTA
·
2020
·
IF 5.339
Ruipeng Chen,Yunfeng Sun,Bingyang Huo,Xudong Zhao,Hui Huang,Shuang Li,Jialei Bai,Jun Liang,Zhixian Gao
The mycotoxin ochratoxin A (OTA) is a secondary metabolite derived from multiple Aspergillus and Penicillium strains. The development of a rapid, sensitive, and simple method for OTA detection is important to ensure food biosafety and safeguard public health. In this study, we designed a highly specific and sensitive assay for the detection of OTA using copper monosulfide (CuS) nanoparticles conjugated to an anti-OTA antibody (CuS-Ab NPs) and a fluorescent probe for Cu 2+ . When OTA is present in the solution, the OTA antigen, bound to the microplate, is competed off by the soluble OTA for binding to CuS-Ab NPs. After washing, the CuS-Ab NPs and bound OTA are removed. Subsequently, HCl is added to dissolve the CuS-Ab NPs bound to the OTA antigen, releasing Cu 2+ and activating the Cu 2+ fluorescent probe . Thus, the resultant fluorescence emission is inversely proportional to the OTA content in the solution. Under optimal conditions, this method detected 0.1–100 ng mL −1 OTA with a limit of detection of 0.01 ng mL −1 . The assay was tested using corn, soybean, and coffee samples, with recoveries ranging from 94% to 110%. This strategy provides a new approach for the detection of mycotoxins and other small-molecule analytes with broad application potential in food safety and quality control.
研究领域:
食品科学
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分析化学
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生物技术
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PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xin Xie,Jingxi Wang,Daxiu Li,Ding Wang
Ochratoxin A (OTA) poses a great threat to food and environmental safety owing to its high toxicity. Herein, we developed a sensitive electrochemical aptasensor that integrates aptamer, enzyme-free DNA circuits, and electrochemical technology for OTA detection. In the presence of target OTA, the aptamer recognition first initiated the EDC circuit to releasing DNA (T2) from a ternary DNA complex (S-O-T2). The released T2 as the trigger to activate the CHA circuit and expose amounts of G-quadruplex sequences on modified electrode surface. Eventually, hemin was embedded in the G-quadruplex structure and generated an amplified electrochemical signal. Under optimal conditions, this developed biosensor is capable of detecting OTA ranging from 1 pg/mL to 10 ng/mL with a low detection limit of 0.135 pg/mL, which could be contributed to the design of target-triggered EDC and CHA cascade amplification. Additionally, the proposed biosensor was applied to analyze OTA in corn samples with satisfactory results, demonstrating that the proposed method has great potential in food safety and environmental monitoring.
研究领域:
生物化学
·
分析化学
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食品科学
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xiaolong Zheng,Linlin Sun,Yanan Zhao,Hualin Yang,Yuanhua Zhu,Junxiang Zhang,Die Xu,Xingping Zhang,Yu Zhou
Ochratoxin A (OTA) is a widely distributed mycotoxin that seriously endangers food safety and human health. Therefore, establishing a sensitive and simple method for detecting OTA is crucial to ensure food safety. A fluorescence and colorimetric dual-mode immunoassay was established using cerium-based nanoparticles CPNs(Ⅳ) for detection of OTA. CPNs(IV) with good oxidase activity can catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to produce visible blue color signal. Under the catalysis of ALP, the ascorbic acid 2-phosphate (AA2P) was hydrolyzed to form ascorbic acid (AA), which reduced CPNs(Ⅳ) to CPNs(III). The produced CPNs(III) can emit significant fluorescence signal. Because the concentration of OTA was inversely proportional to the concentration of IgG-ALP in the microplate, the fluorescence signal was gradually decreased and the blue color signal gradually increased with the addition of OTA. The limits of detection (LODs) of this dual-mode immunoassay were 0.404 ng/mL (for fluorescent mode) and 0.962 ng/mL (for colorimetric mode). The recoveries of OTA were 97.60 %–103.55 % for the fluorescence mode and 99.12 %–102.60 % for the colorimetric mode in corn samples. These results indicated the dual-mode immunoassay was sensitive and reliable for detection of OTA in actual samples.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Yonghong Wang,Zeyang Fang,Ge Ning,Shaoming Mao,Yaohui Wu,Shun Wu,Gao-Qiang Liu
After successively introducing elements of a triple-helix aptamer probe (TAP) and a luminol–hydrogen peroxide (H 2 O 2 ) solution, we proposed a brand-new chemiluminescence (CL)-based biosensor for the assay of ochratoxin A (OTA). Primarily, the TAP contains an aptamer loop, two DNA segment stems, and a triplex-forming oligonucleotide (signal probe). In the presence of a target, the aptamer sequence binds to the target with an impressive affinity. This association leads to a conformational change in the TAP, thus releasing the signal probe. Sequentially, with the participation of hemin and potassium ions, the dissociated probe forms into a G-quadruplex structure that is capable of catalyzing luminol to produce CL mediated by H 2 O 2 . In contrast, in the absence of the target, a low background signal remains. Using this approach of combining a novel TAP switch with a G-quadruplex-catalyzed luminol-H 2 O 2 system, we presented a label-free and signal-on CL strategy for the measurement of OTA. Under optimal conditions, the gradually strengthened signal maintained a linear dependence on target concentrations ranging from 0.1 ng/mL to 2.0 ng/mL, and the detection limit was as low as 0.07 ng/mL. This newly designed, simple, and cost-effective protocol affords high sensitivity, specificity, suitability, and efficacy for biosensing. Thus, this protocol is attractive for a range of target quantifications, with only the matched aptamer requiring replacement (no redesign of signal probes).
研究领域:
分析化学
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食品安全
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生物技术
·
分子诊断
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高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 11
·1-8
·
IF 5.705
Wu Shijia,Liu Lihong,Duan Nuo,Wang Wenyue,Yu Qianru,Wang Zhouping
An aptamer-based test strip is described for visual and instrumental determination of the mycotoxin ochratoxin A (OTA). It is based on the use of NaYF 4 :Yb,Er upconversion nanoparticles (UCNPs) as a label for the aptamer and on the competition between OTA and its complementary sequence for an OTA-specific aptamer. To improve the analytical performance, the optical properties of the UCNPs, the fluidity of the UCNP-aptamer conjugate, and the migration rate on the nitrocellulose membranes were investigated. Under the optimal experimental conditions and by using a 980-nm laser, the relative fluorescence intensity (test line value/control line value) is proportional to the logarithm of the OTA concentration over a range from 5 to 100 ng·mL −1 (R 2 = 0.9955). The limit of the detection is 1.86 ng·mL −1 . This aptamer based flow assay can be performed within 15 min and has no serious cross-sensitivity to potentially interfering species. It was successfully applied to the determination of OTA in spiked wheat and beer samples.
An aptamer-based upconversion fluorescent strip based on the use of NaYF 4 :Yb,Er nanoparticles was developed for sensitive detection of Ochratoxin A. The limit of the detection was determined as 1.86 ng·mL −1 . The assay can be performed within 15 min, indicating its great potential in point-of-care testing.
研究领域:
分析化学
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毒理学
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食品科学
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高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Wenting Li,Xinai Zhang,Xuetao Hu,Yongqiang Shi,Nini Liang,Xiaowei Huang,Xin Wang,Tingting Shen,Xiaobo Zou,Jiyong Shi
A simple fluorescence and electrochemical dual-channel biosensor based on bifunctional Zr(IV)-based metal–organic framework (Zr-MOF) was proposed to detect Ochratoxin A (OTA). The bifunctional Zr-MOF, with photoluminescence properties and enormous electroactive ligands, was exploited to load OTA-specific aptamers for designing signal probes, greatly simplifying the probe-fabrication process and improving sensing reliability. Upon specific recognition of aptamer toward OTA, the anchored probe was released from the sensing interface into the reaction solution. In this circumstance, the increased amount of the signal probe in reaction solution led to an enhanced fluorescence response, while the decreased amount of the signal probe on the sensing interface resulted in a diminished electrochemical response. According to the dual-channel signal change with increasing OTA concentration, the visual fluorescence strategy was established for intuitive OTA detection, and meanwhile, sensitive electrochemical assay with a detection limit of 0.024 pg/mL was also achieved with the help of one-step electrodeposition as a sensing platform. Moreover, the proposed dual-channel assay has been successfully applied to determine OTA levels in corn samples with rapid response, superior accuracy, and high anti-interference capability, providing a promising method for food safety monitoring.
研究领域:
食品安全
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分析化学
·
生物传感
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PubMed
高影响因子
Journal of the American Chemical Society
·
2022
·
IF 16.383
Guohua Xu,Jiajing Zhao,Hao Yu,Chen Wang,Yangyu Huang,Qiang Zhao,Xin Zhou,Conggang Li,Maili Liu
A 36-mer guanine (G)-rich DNA aptamer (OBA36) is able to distinguish one atomic difference between ochratoxin analogues A (OTA) and B (OTB), showing prominent recognition specificity and affinity among hundreds of aptamers for small molecules. Why OBA36 has >100-fold higher binding affinity to OTA than OTB remains a long-standing question due to the lack of high-resolution structure. Here we report the solution NMR structure of the aptamer–OTA complex. It was found that OTA binding induces the aptamer to fold into a well-defined unique duplex–quadruplex structural scaffold stabilized by Mg2+ and Na+ ions. OTA does not directly interact with the G-quadruplex, but specifically binds at the junction between the double helix and G-quadruplex through π–π stacking, halogen bonding (X-bond), and hydrophobic interaction. OTB has the same binding site as OTA but lacks the X-bond. The strong X-bond formed between the chlorine atom of OTA and the aromatic ring of C5 is the key to discriminating the strong binding toward OTA. The present research contributes to a deeper insight of aptamer molecular recognition, reveals structural basis of the high-affinity binding of aptamers, and provides a foundation for further aptamer engineering and applications.
研究领域:
生物化学
·
分子生物学
·
结构生物学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Rui Chen,Yongzhen Dong,Feng Hong,Xiya Zhang,Xiaohong Wang,Jia Wang,Yiping Chen
A rapid and accurate detection method is needed for the quantitation of ochratoxin A in agricultural products due to its high toxicity. A microparticle-counting immunosensor based on polydopamine nanoparticle-mediated click chemistry was established for the highly-sensitive detection of ochratoxin A. Polydopamine nanoparticles with good biocompatibility and a strong metal-chelating ability were synthesized and conjugated with the antibody. The Coupled compounds were then used as an immune carrier to change the Cu 2+ concentration via an immuno-reaction. Some of the remaining Cu 2+ ions were reduced to Cu + ions, which caused azide-polystyrene microspheres and alkyne-polystyrene microspheres to aggregate via a Cu + ion-mediated click reaction. Particle counting was used to distinguish changes in the sizes of the polystyrene microspheres from dispersed to aggregated to detect ochratoxin A. It showed a wide linear detection range of 0.5–800 ng/mL, and a detection limit of 0.2 ng/mL. This assay provides an attractive analytical tool for the accurate detection of trace targets in complex samples.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Dongyan Li,Huaiyue Xia,Yingying Sun,Wenjie Liu,Wen Liu,Jianna Yu,Guoxing Jing,Jian Zhang,Wenshan Li
Ochratoxin A(OTA), a highly toxic mycotoxin commonly found in food, poses a serious threat to health even at low concentrations. Developing a sensitive, accurate, simple, and cost-effective detection method is of great significance for food safety and quality control. Herein, a triple cascade amplification strategy was used to construct the colorimetric assay for the detection of OTA, where the amplification process consists of an entropy-driven DNA circuit (EDC), a catalytic hairpin assembly (CHA), and Mg 2+ -assisted DNAzyme catalysis (MNAzyme). Through the specific binding of ochratoxin A (OTA) and its aptamer , an initiator strand is released to initiate upstream EDC and then produce a new trigger unit that motivates downstream CHA to generate MNAzyme, which further cleaves the substrate strand to induce the formation of G-quadruplex/hemin DNAzyme as a signal readout. The aptasensor was shown to detect OTA, with a low detection limit of 8.7 fM and good selectivity . The developed method could be used as a highly colorimetric aptasensor for the detection of OTA in spiked rice samples.
研究领域:
食品安全
·
分析化学
·
分子生物学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jie Zhang,Yuheng Lu,Wei Gao,Peng Yang,Nansheng Cheng,Yanwen Jin,Junbo Chen
Ochratoxin A (OTA) is usually found in the contaminated cereal food that its trace concentration would cause severe carcinogenicity and neurotoxicity. Herein, a recognition-triggered structure switching-based element spectrometric assay was proposed for ultrasensitive and highly specific detection of OTA. OTA aptamer was first adapted into a primer for the formation of a locked hairpin (LHP), and followed by attachment on the surface of magnetic beads (MBs). In the presence of OTA, the binding event between OTA molecule and its aptamer induced the liberation of the primer for that is otherwise unable to spontaneously structure-switching. For the rolling circle amplification (RCA), thereby a dumbbell structure was designed as the template (DT). The hybridization of the dumbbell template with free primer initiates the rolling circle amplification along with the production of repeated sequences. To obtain the signal output, the DNA-AuNP tag was prepared which contained the complementary sequences with RCA products. Thus, each tag can hybridize with the repeated sequences derived from the RCA, enabling the formation of detectable signal by the following ICP-MS measurement. Such design can translate a single recognition event into numerous AuNPs, enabling amplified detection of OTA. Taking advantage of this principle, the OTA in real sample (AFP, PSA) can be quantified at femtomolar concentration and it can be expanded into application in food safety inspection.
研究领域:
食品安全
·
分析化学
·
毒理学
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高影响因子
危险品化学品经营许可证（带存储）