ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Xianli Wang,Peiqi Shen,Nannan Gu,Yi Shao,Mengmeng Lu,Chunbo Tang,Cheng Wang,Chenglin Chu,Feng Xue,Jing Bai
Commercially available guided bone regeneration (GBR) membranes often exhibit limited mechanical properties or bioactivity, leading to poor performance in repairing bone defects. To surmount this limitation, we developed a Janus structural composite membrane (Mg-MgO/PCL) reinforced by dual Mg (Mg sheets and MgO NPs) by using a combined processing technique involving casting and electrospinning. Results showed that the addition of Mg sheets and MgO NPs enhanced the mechanical properties of the composite membrane for osteogenic space maintenance, specifically tensile strength (from 10.2 ± 1.2 to 50.3 ± 4.5 MPa) and compression force (from 0 to 0.94 ± 0.09 N mm–1), through Mg sheet reinforcement and improved crystallization. The dense cast side of the Janus structure membrane displayed better fibroblast barrier capacity than a single fiber structure; meanwhile, the PCL matrix protected the Mg sheet from severe corrosion due to predeformation. The porous microfibers side supported preosteoblast cell adhesion, enhanced osteogenesis, and angiogenesis in vitro, through the biomimetic extracellular matrix and sustainable Mg2+ release. Furthermore, the Mg-MgO/PCL membrane incorporating 2 wt % MgO NPs exhibited remarkable antimicrobial properties, inducing over 88.75% apoptosis in Staphylococcus aureus. An in vivo experiment using the rat skull defect model (Φ = 5 mm) confirmed that the Mg-MgO/PCL membrane significantly improved new bone formation postsurgery. Collectively, our investigation provides valuable insights into the design of multifunctional membranes for clinical oral GBR application.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
骨科
·
牙科科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zehua Feng,Shuang Qiu,Martin Michálek,Yi Zhou,Huiqing Gou,Lu Li,Kai Zheng,Yan Xu
Repairing critical alveolar bone defects in inflammatory microenvironments is challenging. To address this, dual-functional nanofibrous scaffolds with anti-inflammatory and osteogenic properties were developed. These scaffolds incorporate polycaprolactone (PCL) short fibers and mesoporous bioactive glass nanoparticles (MBGNs), fabricated through electrospinning and sol-gel processes, respectively. 3D Fibrous scaffolds were achieved through sequential dispersion, homogenization, thermal self-assembly, and surface modification. During this process, gelatin facilitated the self-assembly of the scaffold, while carboxymethyl chitosan was used to integrate MBGN into the PCL scaffolds. Characterization confirmed a porous structure of scaffolds and enhanced mechanical and in vitro acellular properties after MBGN incorporation. Non-cytotoxicity was confirmed by CCK-8 and Live/Dead assays. Flow cytometry showed scaffold-induced macrophage polarization from M1 (pro-inflammatory) to M2 (anti-inflammatory), supported by qRT-PCR and ELISA, which showed reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines. In co-culture with human bone marrow mesenchymal stem cells, the scaffolds promoted osteogenic differentiation and mineralization, as shown by ALP/ARS staining and increased expression of osteogenic markers (RUNX2, OCN). These findings highlight the dual immunomodulatory and osteogenic functions of the scaffolds, suggesting their potential as a promising strategy for inflammatory alveolar bone regeneration.
研究领域:
生物材料
·
组织工程
·
再生医学
·
生物医学工程
·
纳米技术
·
免疫调节
·
骨修复
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2019
·
IF 2.121
Huilin Ye,Junjin Zhu,Dan Deng,Shue Jin,Jidong Li,Yi Man
Membranes play pivotal role in guided bone regeneration (GBR) technique for reconstruction alveolar bone. GBR membrane that is able to stimulate both osteogenic and angiogenic differentiation of cells may be more effective in clinic practice. Herein, we fabricated the Sr-doped calcium phosphate/polycaprolactone/chitosan (Sr-CaP/PCL/CS) nanohybrid fibrous membrane by incorporating 20 wt% bioactive Sr-CaP nanoparticles into PCL/CS matrix via one-step electrospinning method, in order to endow the membrane with stimulation of osteogenesis and angiogenesis. The physicochemical properties, mechanical properties, Sr2+ release behavior, and the membrane stimulate bone mesenchymal stem cell (BMSCs) differentiation were evaluated in comparison with PCL/CS and CaP/PCL/CS membranes. The SEM images revealed that the nanocomposite membrane mimicked the extracellular matrix structure. The release curve presented a 28-day long continuous release of Sr2+ and concentration which was certified in an optimal range for positive biological effects at each timepoint. The in vitro cell culture experiments certified that the Sr-CaP/PCL/CS membrane enjoyed excellent biocompatibility and remarkably promoted rat bone mesenchymal stem cell (BMSCs) adhesion and proliferation. In terms of osteogenic differentiation, BMSCs seeded on the Sr-CaP/PCL/CS membrane showed a higher ALP activity level and a better matrix mineralization. What’s more, the synergism of the Sr2+ and CaP from the Sr-CaP/PCL/CS membrane enhanced BMSCs angiogenic differentiation, herein resulting in the largest VEGF secretion amount. Consequently, the Sr-CaP/PCL/CS nanohybrid electrospun membrane has promising applications in GBR.
研究领域:
材料科学
·
生物材料
·
组织工程
·
生物医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2025
·
IF 3.6
Ling Peng,Yusong Pan,Qianqian Liu,Cuilian Wei,Run Huang,Wenjie Luo,Xiuling Lin,Jun Tao,Yinghai Xie
Spinal cord injury (SCI) is a common disease worldwide. Tissue engineering scaffolds are considered a promising strategy for SCI treatment, but their efficacy is significantly limited by the local abundance of MSC (mesenchymal stem cell) and a highly inflammatory microenvironment. In this study, a multifunctional composite scaffold of polycaprolactone/chitosan (PCL/CS) loading polydopamine (PDA)-coated nerve growth factor (NGF) loaded onto was prepared using a freeze-drying method. The PCL/CS composite scaffold exhibited tunable degradation properties and excellent biocompatibility. The PDA coating demonstrated potent free radical scavenging capabilities, achieving clearance rate of 90.65%, 77.53%, and 60.77% for DPPH, H2O2, and ·OH radicals, respectively. Remarkably, the scaffold effectively reduced intracellular ROS levels within 24 h under oxidative stress conditions. Flow cytometry revealed rapid cellular internalization, with the FITC-labeled PC-5-PDA group exhibiting a 99.87% FITC+ rate within 12 h. NGF release from the scaffold induced robust neuronal differentiation of PC-12 cells, as evidenced by a 58.1% increase in neurite length (from 104.77 μm at day 3 to 165.66 μm at day 4) and upregulated expression of microtubule-associated protein 2 (MAP2). These findings demonstrate the dual functionality of the scaffold, which is effectively modulating the post-SCI inflammatory microenvironment while promoting neural regeneration.
研究领域:
生物材料
·
组织工程
·
神经再生
·
抗氧化治疗
·
干细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaoci Cui,Yingying Zhang,Zhiyuan Chen,Huining Xiao,Ranhua Xiong,Chaobo Huang
Water and soil pollution caused by Cu 2+ is not conducive to sustainable development of environment and could cause damage to environment and even human body. Currently, fluorescent sensor solutions analysis method has been used for Cu 2+ detection, but they also suffer from drawbacks including easy leakage, difficult storage, and inaccurate. Herein, a green solid-state biomass fluorescence platform (NBU-CDs) consisting of xylan-derived carbon dots (U-CDs) and polylactic acid/polycaprolactone (PLA/PCL) was designed by using in situ electrospinning technology. The prepared NBU-CDs fluorescence platform showed good fluorescence effect and can be served as fluorescence sensor for detecting Cu 2+ with high sensitively, selectively and low detection limit (LOD = 0.83 μM). The practical applications of NBU-CDs exhibited high specificity for Cu 2+ detection in zebrafish, water samples (school lake, Xuanwu Lake and Yangtze River) with high recovery rates of 97 %–104 % and soil (pond soil, grassland soil and bamboo soil) samples, respectively. The developed fluorescence platform was utilized to predict water and soil safety by monitoring Cu 2+ concentration and provides a new strategy for Cu 2+ detection.
研究领域:
环境科学
·
材料科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Hebin Ma,Bowen Xie,Hongguang Chen,Lifang Hao,Haigang Jia,Dengjie Yu,Yuanbo Zhou,Puzhen Song,Yajing Li,Jing Liu,Kaitao Yu,Yantao Zhao,Yadong Zhang
A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. Advances in tissue engineering have introduced meniscal tissue engineering as a potential treatment option. In this study, we established the size of a standardized meniscal scaffold using knee Magnetic Resonance Imaging (MRI) data and created a precise Polycaprolactone (PCL) scaffold utilizing 3-Dimensional (3D) printing technology, which was then combined with Fibrin (Fib) hydrogel to form a PCL-Fib scaffold. The PCL scaffold offers superior biomechanical properties, while the Fib hydrogel creates a conducive microenvironment for cell growth, supporting chondrocyte proliferation and extracellular matrix (ECM) production. Physical and chemical characterization, biocompatibility testing, and in vivo animal experiments revealed the excellent biomechanical properties and biocompatibility of the scaffold, which enhanced in situ meniscal regeneration and reduced osteoarthritis progression. In conclusion, the integration of 3D printing technology and the Fib hydrogel provided a supportive microenvironment for chondrocyte proliferation and ECM secretion, facilitating the in situ regeneration and repair of the meniscal defect. This innovative approach presents a promising avenue for meniscal injury treatment and advances the clinical utilization of artificial meniscal grafts.
研究领域:
组织工程
·
骨科
·
生物医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Haozheng Wang,Lei Cui,Ying Luo,Honghong Chen,Xiaoju Liu,Qiang Shi
Delayed endothelialization, the excessive proliferation of smooth muscle cells (SMCs), and persistent inflammation are the main reasons for the implantation failure of blood-contacting materials. To overcome this problem, an inflammation-responsive, core-shell structured microfiber scaffold is developed using polycaprolactone (PCL), selenocystamine-modified gelatin (Gel-Se), L-ascorbyl 6-palmitate (AP), and dexamethasone as the fiber shell, with poly ( l -lysine) (PLL) and heparin incorporated in the fiber core. Superhydrophilic microfiber scaffolds exhibit antifouling properties that inhibit protein adsorption and blood cell adhesion, thereby effectively mitigating the risk of acute thrombosis. The continuous release of heparin and sustained generation of nitric oxide (NO) through the catalytic decomposition of S-nitrosothiols by selenocystamine lead to a biomimetic endothelial function for the enhancement of blood compatibility. The inflammation-responsive compound AP can detoxify excess reactive oxygen species (ROS) while controlling the release of dexamethasone to reduce chronic inflammation. We demonstrate the ability of microfiber scaffolds to reduce thrombotic and inflammatory complications, inhibit SMC proliferation, and promote rapid endothelialization both in vitro and ex vivo. Hence, microfiber scaffolds are robust and promising for blood-contacting implants with enhanced antithrombogenicity and anti-inflammatory capabilities.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2023
·
IF 5
Zhiping Fang,Shaoyue Zhang,Han Wang,Xue Geng,Lin Ye,Ai-ying Zhang,Zeng-guo Feng
The core–shell structured nanofibers are routinely fabricated by coaxial solution and single-nozzle emulsion electrospinning of two polymers. Herein, the core–shell structured polymeric nanofibers were electrospun from mixed solutions of poly(ether urethane) (PEU) with poly(ε-caprolactone) (PCL) or other biodegradable aliphatic polyesters via a single-nozzle spinneret. For comparison, the mixed solutions electrospinning of poly(carbonate urethane) (PCU) with these polyesters including PCL was also conducted. The morphologies and hierarchical structures of the as-spun nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), in vitro lipase degradation, differential scanning calorimeter (DSC), and Fourier-transform infrared (FTIR) analyses. It was shown that the blends of PEU with PCL or other biodegradable aliphatic polyesters were solely electrospun into the core–shell structured nanofibers with PEU as a shell and PCL or other biodegradable aliphatic polyesters as a core. The thickness of core/shell layers from 833/606 to 193.3/54.2 μm was adjustable by varying the feeding mass ratio from 1:3 to 3:1 of PEU to PCL or other biodegradable aliphatic polyesters. In DSC analysis, the Tonset of PEU@PCL core–shell fiber was 1.31 °C higher than that of the PCL fiber, while Tm was approximate. Furthermore, changing the electrospinning solvents of PEU with PCL or other biodegradable aliphatic polyesters retained the formation of core–shell nanofibers. In contrast, the blends of PCU with these polyesters tended to form co-continuous structured nanofibers. The effects of the physicochemical properties of mixed solutions on the charged liquid droplets, whipped jets, and morphology of the electrospun nanofibers were also inspected. The creation of core–shell nanofibers from the blends of PEU with PCL or other biodegradable aliphatic polyesters was most likely due to the interaction between the inherent thermodynamic phase separation of the polymers and their external stretching kinetic phase separation during electrospinning.
研究领域:
材料科学
·
纳米技术
·
高分子科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2020
·
IF 7.242
Yangyang Li,Mingxue Chen,Wenhao Zhou,Shuang Gao,Xujiang Luo,Liqing Peng,Jianglong Yan,Pei Wang,Qiyao Li,Yufeng Zheng,Shuyun Liu,Yan Cheng,Quanyi Guo
Considering the intrinsic poor self-healing capacity of meniscus, tissue engineering has become a new direction for the treatment of meniscus lesions. However, disturbed by mechanical stability and biocompatibility, most meniscus implants fail to relieve symptoms and prevent the development of osteoarthritis. The goal of this study was to develop a potential meniscal substitute for clinical application. Here, silk fibroin with good mechanical performance and biocompatibility, and strontium ion acting as bioactive factor, were incorporated with Ɛ-Polycaprolactone to fabricate a meniscus scaffold (SP-Sr). By the wet-electrospun method, the 3D SP-Sr provided suitable pore size (100–200 μm) and enough mechanical support (61.6 ± 2.9 MPa for tensile modulus and 0.11 ± 0.03 MPa for compressive modulus). Moreover, after addition of Sr 2+ , the SP-Sr seeded by rabbit adipose tissue-derived stromal cells (rADSCs) showed the highest secretion with 2.61- and 2.98-fold increase in collagen and aggrecan , respectively, compared with SF/PCL group. And the extracellular matrix related genes expression in SP-Sr also showed upregulation results. Particularly, the expression of the collagen II gene, which played a crucial role in the formation of meniscal inner avascular region, showed a 9-fold increase in SP-Sr compared with pure PCL group. Furthermore, the MRI results of SP-Sr implanted in rabbits with total meniscectomy for 6 months demonstrated effective prevention of meniscus extrusion and relieving joint space narrowing compared with meniscectomy group. And the effects of cartilage protection and delaying osteoarthritis development were confirmed by Pathological examination. Especially, after 6-month implantation, the neo-menisci showed similar structural constituent and mechanical performance. Statement of significance Meniscus regeneration faces great challenge due to the meniscus having limited healing potential owing to its anisotropic structure, its hypocellularity and hypovascularity. The present tissue engineering solutions have failed to maintain the biological function for meniscus reconstruction in vivo because of fragile and poor biocompatible materials, leading to long-term joint degeneration. The goal of this study was to develop a meniscal substitute potential for clinical application. Here, silk fibroin and strontium were incorporated with Ɛ-Polycaprolactone by wet-electrospinning method to fabricate a meniscus scaffold (SP-Sr). The 6-month implantation results revealed that SP-Sr scaffold was effective in preventing meniscus extrusion, cartilage protection and delaying osteoarthritis development, and the regenerated menisci showed similar structural constituent and mechanical performance.
研究领域:
组织工程
·
骨科
·
再生医学
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 32
·27102-27112
·
IF 3.289
Lei Li,Beibei Lu,Qikui Fan,Lulu Wei,Jianning Wu,Jun Hou,Xuhong Guo,Zhiyong Liu
In this work, well-defined pH-responsive methoxy poly(ethylene glycol)-block-poly(ε-caprolactone)-block-poly[2-(dimethylamino)ethyl methacrylate]-g-7-propinyloxy coumarin triblock amphiphilic copolymers (mPEG-b-PCL-b-PDMAEMA-g-PC) were synthesized using a combination of atom transfer radical polymerization (ATRP), ring opening polymerization (ROP) and click chemistry. The chemical structures and compositions of these copolymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance (1H NMR). The molecular weights of the copolymers were obtained using 1H NMR spectroscopy and gel permeation chromatography (GPC) measurements. Subsequently, the polymers could self-assemble into micelles which were investigated using dynamic light scattering (DLS), transmission electron microscopy (TEM), and fluorescence spectroscopy. The pH-responsive self-assembly behavior of these triblock copolymers in water were investigated at different pH values of 5 and 7.4 for controlled doxorubicin release, the results indicated that the release rate of DOX could be effectively controlled by altering the pH, DOX was sealed in neutral surroundings and DOX release was triggered in acidic surroundings. CCK-8 assays and confocal laser scanning microscopy (CLSM) against HeLa cells indicated that the micelles had no associated cytotoxicity, possessed good biodegradability and biocompatibility, and identified the location of the DOX in HeLa cells. The DOX-loaded micelles possessed high cytotoxicity to HeLa cells and exhibited inhibition of the proliferation of HeLa cells. Moreover, these flexible micelles with an on–off switched drug release may offer a promising pattern to deliver a wide variety of hydrophobic payloads to tumor cells for cancer therapy.
研究领域:
材料科学
·
纳米技术
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Linzhi Jing,Xiang Wang,Bin Leng,Ningping Zhan,Hang Liu,Shifei Wang,Yuyun Lu,Jie Sun,Dejian Huang
Scaffold-based three-dimensional (3D) cell culture systems have gained increased interest in cell biology, tissue engineering, and drug screening fields as a replacement of two-dimensional (2D) monolayer cell culture and as a way to provide biomimetic extracellular matrix environments. In this study, microscale fibrous scaffolds were fabricated via electrohydrodynamic printing, and nanoscale features were created on the fiber surface by simply leaching gliadin of poly(ε-caprolactone) (PCL)/gliadin composites in ethanol solution. The microstructure of the printed scaffolds could be precisely controlled by printing parameters, and the surface nanotopography of the printed fiber could be tuned by varying the PCL/gliadin ratios. By seeding mouse embryonic fibroblast (NIH/3T3) cells and human nonsmall cell lung cancer (A549) cells on the printed scaffolds, the cellular responses showed that the fiber nanotopography on printed scaffolds efficiently favored cell adhesion, migration, proliferation, and tissue formation. Quantitative analysis of the transcript expression levels of A549 cells seeded on nanoporous scaffolds further revealed the upregulation of integrin-β1, focal adhesion kinase, Ki-67, E-cadherin, and epithelial growth factor receptors over what was observed in the cells grown on the pure PCL scaffold. Furthermore, a significant difference was found in the relevant biomarker expression on the developed scaffolds compared with that in the monolayer culture, demonstrating the potential of cancer cell-seeded scaffolds as 3D in vitro tumor models for cancer research and drug screening.
研究领域:
组织工程
·
细胞生物学
·
癌症研究
·
药物筛选
✓ 已验证
产品关联
DOI
PubMed
EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Donglei Liu,Jiaxin Han,Xijing Zhuang,Yang Gao,Wenjun Wang,Dan Qin,Xufeng Dong
A three-layer small-diameter vascular graft (SDVG) of polycaprolactone (PCL) and l -polylactic acid (PLLA) with pore diameter gradient was prepared through combining variable-extrusion speed iterative electrospinning and ultrasonic reaming. The SEM results showed that the three-layer PCL/PLLA SDVG has distinct pore diameter gradient. The Young’s modulus, ultimate tensile strength (UTS), burst pressure (BP) and compliance of the vascular graft were high as 20.116 ± 0.465 MPa, 2.259 ± 0.141 MPa, 2050 ± 249 mmHg and 4.203 ± 0.330 %/100 mmHg, respectively. The UTS of the graft is similar to human coronary arteries. The BP meets the basic requirements to prevent aneurysmal dilation, and the compliance is higher than that of sheep carotid artery. The fluid–solid coupling simulation results showed that the graft had high mechanical properties to withstand the same blood flow velocity as human coronary arteries, and there was no stress concentration in the wall. Besides, it had good biocompatibility, promoting the adhesion and proliferation of rat vascular endothelial cells (ECs). The successful preparation of the small-diameter vascular graft with high mechanical properties for preventing itself from rupturing and ensuring the overall function, provides more possibilities for the clinical transplantation of small-diameter vascular grafts.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）