International Journal of Nanomedicine
·
2023
·
IF 8
Chenchen Bi,Aiping Wang,Yongchao Chu,Sha Liu,Hongjie Mu,Wanhui Liu,Zimei Wu,Kaoxiang Sun,Youxin Li
Sustainable and safe delivery of brain-targeted drugs is highly important for successful therapy in Parkinson’s disease (PD). This study was designed to formulate biodegradable poly(ethylene glycol)–poly(lactic-co-glycolic acid) (PEG-PLGA) nanoparticles (NPs), which were surface-modified with lactoferrin (Lf), for efficient intranasal delivery of rotigotine to the brain for the treatment of PD. Rotigotine NPs were prepared by nanoprecipitation, and the effect of various independent process variables on the resulting properties of NPs was investigated by a Box–Behnken experimental design. The physicochemical and pharmaceutical properties of the NPs and Lf-NPs were characterized, and the release kinetics suggested that both NPs and Lf-NPs provided continuous, slow release of rotigotine for 48 h. Neither rotigotine NPs nor Lf-NPs reduced the viability of 16HBE and SH-SY5Y cells; in contrast, free rotigotine was cytotoxic. Qualitative and quantitative cellular uptake studies demonstrated that accumulation of Lf-NPs was greater than that of NPs in 16HBE and SH-SY5Y cells. Following intranasal administration, brain delivery of rotigotine was much more effective with Lf-NPs than with NPs. The brain distribution of rotigotine was heterogeneous, with a higher concentration in the striatum, the primary region affected in PD. This strongly suggested that Lf-NPs enable the targeted delivery of rotigotine for the treatment of PD. Taken together, these results demonstrated that Lf-NPs have potential as a carrier for nose-to-brain delivery of rotigotine for the treatment of PD.
研究领域:
药剂学
·
神经科学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2021
·
IF 4.749
Jidong Dai,Xin Tan,Min Liang,Dandan Wei,Yinhua Tao,Pengfei Ren,Tianzhu Zhang
Poly(lactide-co-glycolide) (PLGA) has been widely used as a scaffold material for tissue engineering owing to its biocompatibility, biodegradability, and biosafety. However, lactic acid (LA) produced during PLGA degradation is prone to inflammation, which is a shortcoming that must be avoided. To this end, crystalline PLGA-PEG was synthesized here for the first time. To make the crystalline PLGA-PEG more suitable for tissue engineering, porous crystalline PLGA-PEG was prepared via the swelling behavior during recrystallization annealing. The structure and properties of the porous crystalline PLGA-PEG were confirmed by SEM, POM, and XRD. Furthermore, the swelling behavior of different PEG molecular weights was studied, and the cell viability test and alkaline phosphatase activity test showed that PLGA-PEG has good biocompatibility. Such a porous crystalline PLGA-PEG will make PLGA have a broader application prospect in bone repair.
研究领域:
材料科学
·
生物医学工程
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Renqi Guo,Ying Zhou,Siju Liu,Chuang Li,Cuifen Lu,Guichun Yang,Junqi Nie,Feiyi Wang,Nian-Guo Dong,Jiawei Shi
Calcification of bioprosthetics is a primary challenge in the field of artificial heart valves and a main reason for biological heart valve prostheses failure. Recent advances in nanomaterial science have promoted the development of polymers with advantageous properties that are likely suitable for artificial heart valves. In this work, we developed a nanocomposite polymeric biomaterial POSS–PEG (polyhedral oligomeric silsesquioxane-polyethylene glycol) hybrid hydrogel, which not only has improved mechanical and surface properties but also excellent biocompatibility. The results of atomic force microscopy and in vivo animal experiments indicated that the content of POSS in the PEG matrix plays an important role on the surface and contributes to its biological properties, compared to the decellularized porcine aortic valve scaffold. Additionally, this modification leads to enhanced protection of the hydrogel from thrombosis. Furthermore, the introduction of POSS nanoparticles also gives the hydrogel a better calcification resistance efficacy, which was confirmed through in vitro tests and animal experiments. These findings indicate that POSS–PEG hybrid hydrogel is a potential material for functional heart valve prosthetics, and the use of POSS nanocomposites in artificial valves may offer potential long-term performance and durability advantages.
研究领域:
生物医学工程
·
心血管医学
·
材料科学
✓ 已验证
DOI
PubMed
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2012
·
IF 3.212
Jianian Chen,Shaoshun Li,Qi Shen
Dual- and multi-functional drug delivery systems , especially ligand-modified nanoparticles (NPs) loaded with chemotherapeutic agents are paid much attention to due to their excellent behavior in vitro and in vivo . Bifunctional NPs (BF-NPs), which were based on PLGA–PEG and modified with folic acid and cell penetrating peptide R 7 simultaneously, were developed. BF-NPs loaded with vincristine sulfate (VCR) were prepared via the water–oil–water emulsion solvent evaporation method. BF-NPs showed favorable particle size and zeta potentials , promising drug loading and entrapment efficiency. The release of VCR from BF-NPs exhibited a biphase release manner. Cellular uptake of BF-NPs was found to be higher than that of the NPs merely modified by folic acid or R 7 . In vitro cytotoxicity, cell apoptosis and cell cycle arrest studies also revealed that BF-NPs were more potent than those of the NPs merely modified by folic acid or R 7 . Therefore, the results demonstrated that BF-NPs developed in this study could be a potential vehicle for delivering chemotherapeutic agents such as VCR and breast cancer therapy.
研究领域:
药学
·
纳米技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2020
·
IF 3.557
Shaoqiao Li,Zhuliu Xiao,Kairui Guo,Huihui Gan,Jirong Wang,Yong Zhang,Liping Yu,Zhigang Xue
Porous membranes fabricated from poly(vinylidene fluoride) (PVDF) and a star polymer with linear poly(ethylene glycol) (PEG) arms and cycloPEG cores were fabricated via the phase-separation method. The porous gel polymer electrolytes (PGPEs) were obtained by immersing the porous membranes in the electrolyte solution. When the additive amount of star polymer was up to 20 wt %, the prepared membrane had the largest porosity and the pores were uniformly distributed in the membrane. The star polymer can not only decrease the crystallization of PVDF and enhance the absorption of liquid electrolyte but also offer ion conduction channels (cycloPEG cores). Therefore, the PGPE with 20 wt % star polymers exhibited competitive ionic conductivities of 1.27 mS cm–1 at 30 °C and 2.89 mS cm–1 at 80 °C. To stabilize the liquid electrolyte in the holes of porous membranes, a gelator was introduced in the liquid electrolyte to form gelled porous gel polymer electrolytes (GPGPEs), and the leakage of liquid electrolytes was thus remarkably reduced. The ionic conductivity of GPGPEs with 20 wt % star polymer and 1.5 wt % gelator was importantly improved at high temperatures (6.02 mS cm–1 at 80 °C). We systematically investigated the electrochemical performances of PGPEs without star polymer, PGPEs with star polymer, and GPGPEs with star polymer. The incorporation of star polymers with linear PEG arms and cycloPEG cores into the PGPEs and GPGPEs significantly improved the electrochemical performances of the lithium metal/LiFePO4 cell assembled with the PGPEs or GPGPEs.
研究领域:
材料科学
·
电化学
·
储能技术
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Yijia Yu,Wenjuan Xia,Wenjin Wang,Zhaoqiang Wu,Hong Chen
Hydrophilic antifouling polymers provide excellent antifouling effects under usual short-term use conditions, but the long-term accumulation of contaminants causes them to lose their antifouling properties. To overcome this drawback, surface-initiated ring-opening graft polymerization (SI-ROP) was performed on the surface of the material by applying the cyclic carbide monomer 4′-(fluorosulfonyl)benzyl-5-methyl-2-oxo-1,3-dioxane-5-carboxylate (FMC), which contains a sulfonylfluoride group on the side chain, followed by a “sulfur(IV)-fluorine exchange” (SuFEx) post click modification reaction to link the hydrophilic polyethylene glycol (PEG) to the polyFMC (PFMC) brush, and a novel antifouling strategy for self-polishing dynamic antifouling surfaces was developed. The experimental results showed that the antifouling surface could effectively prevent the adsorption of proteins such as bovine serum albumin (BSA, ∼96.4%), fibrinogen (Fg, ∼87.8%) and lysozyme (Lyz ∼69.4%) as well as the adhesion of microorganisms such as the bacteria Staphylococcus aureus ( S. aureus ) (∼87.5%) and HeLa cells (∼67.2%). Moreover, the enzymatically self-polished surface still has excellent antifouling properties. Therefore, this modification method has potential applications in the field of biosensors and novel antifouling materials.
研究领域:
材料科学
·
表面化学
·
生物医学工程
·
高分子科学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 4
·2613-2619
·
IF 1.483
Feng Zhao,Qi Jianqi,Huang Zhangyi,Xie Xiumin,Wei Nian,Lu Tiecheng
Single-phase nano-crystalline yttria-stabilized zirconia (YSZ) powder was prepared by co-precipitation method using zirconium oxychloride (ZrOCl2 · 8H2O) and yttrium nitrate (Y(NO3)3 · 6H2O) as raw materials and ammonium
bicarbonate (NH4HCO3) and liquor ammonia (NH4OH) as the precipitators. In order to get the powder with favorable dispersibility, polyethylene glycol (PEG, HO(CH2CH2O)
n
H) with different molecular weights and amounts was
used as a dispersant agent in the synthesis process and its effects on the YSZ powder was investigated. The scanning electron micrograph (SEM) images showed that the degree of agglomeration and the mean crystal size of YSZ powder varied with the amount and the molecular weight of PEG. The
appropriate amount and molecular weight of PEG were given by comparing the dispersibility and crystal size distribution of powders. In the process, one also could find that the promotion of dispersion was insensitive to the molecular weight, and all PEG (400–2000) can exert an ability
to reduce the agglomeration. The proper amount of PEG400, PEG600, PEG1000, PEG2000 was 3 wt%, 3 wt%, 2 wt%, 2 wt%, respectively. The transmission electric microscope studies in conjunction with the XRD analyzes verified the low degree of agglomeration and the size of YSZ powder varied between
10~20 nm.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Guangtao Xu,Huan Gu,Bo Hu,Fei Tong,Daojun Liu,Xiaojun Yu,Yongxia Zheng,Jiang Gu
Brain ischemia/reperfusion (I/R) injury (BI/RI) is a leading cause of death and disability worldwide. However, the outcome of pharmacotherapy for BI/RI remains unsatisfactory. Innovative approaches for enhancing drug sensitivity and recovering neuronal activity in BI/RI treatment are urgently needed. The purpose of this study was to evaluate the protective effects of tumor necrosis factor (TNF)-α-loaded poly(ethylene glycol)-b-(poly(ethylenediamine L-glutamate)-g-poly(L-lysine)) (TNF-α/PEG-b-(PELG-g-PLL)) nanoparticles on BI/RI. The particle size of PEG-b-(PELG-g-PLL) and the loading and release rates of TNF-α were determined. The nanoparticle cytotoxicity was evaluated in vitro using rat cortical neurons. Sprague Dawley rats were preconditioned with free TNF-α or TNF-α/PEG-b-(PELG-g-PLL) polyplexes and then subjected to 2 hours ischemia and 22 hours reperfusion. Brain edema was assessed using the brain edema ratio, and the antioxidative activity was assessed by measuring the superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content in the brain tissue. We further estimated the inflammatory activity and apoptosis level by determining the levels of interleukin-4 (IL-4), IL-6, IL-8, IL-10, and nitric oxide (NO), as well as the expression of glial fibrillary acidic protein (GFAP), intercellular adhesion molecule-1 (ICAM-1), and cysteine aspartase-3 (caspase-3), in the brain tissue. We provide evidence that TNF-α preconditioning attenuated the oxidative stress injury, the inflammatory activity, and the apoptosis level in I/R-induced cerebral injury, while the application of block copolymer PEG-b-(PELG-g-PLL) as a potential TNF-α nanocarrier with sustained release significantly enhanced the bioavailability of TNF-α. We propose that the block copolymer PEG-b-(PELG-g-PLL) may function as a potent nanocarrier for augmenting BI/RI pharmacotherapy, with unprecedented clinical benefits. Further studies are needed to better clarify the underlying mechanisms.
研究领域:
药理学
·
神经科学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
Nano Energy
·
2024
·
IF 16.8
Qian Han,Zengyi Fang,Rui Lin,Junyang Chen,Xianhao Wei,Cuicui Gong,Zhixin Yang,Pingjin Zou,Jingyuan Zhu,Lili Xing,Xinyu Xue,Jinyi Lang,Yi Zhou,Meihua Chen
Cancer remains a global health concern, necessitating advanced therapeutic strategies to address its fatal challenges. Conventional treatments, such as radiotherapy and chemotherapy, often lack tumor specificity and may induce systemic toxicity. Photodynamic therapy (PDT) and sonodynamic therapy (SDT) have emerged as promising alternatives, demonstrating high specificity and minimal side effects. In this study, we propose a novel piezo-photodynamic therapy (PPDT) enabled with battery-free wireless cancer therapeutic dot, in which the nanocomposites of gold nanoparticles (Au NPs), zinc oxide nanorods (ZnO NRs) and hydrophilic polyethylene glycol (PEG) can enhance reactive oxygen species (ROS) generation and effectively inhibit tumor growth. The therapeutic dot is implanted directly inside the tissue around the tumor and wirelessly controlled by ultrasound (US), allowing dual activation of electron-hole pairs from the nanocomposites via US and ultraviolet light (UV). The internally generated piezoelectric field minimizes electron-hole recombination, maximizing ROS induction within tumor cells. This PPDT approach surpasses the efficacy of conventional PDT and SDT, showcasing a synergistic therapeutic effect. The study further elucidates the detailed mechanism of Au@PEG-ZnO NRs catalysts, providing insights into their piezoelectric-photocatalytic action. The battery-free wireless control and minimally invasive implantation of the therapeutic dot with inorganic piezo-photocatalyst open new perspectives in cancer therapy.
研究领域:
肿瘤学
·
生物医学工程
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Xiaorong Gou,Meiling Zheng,Yuanyuan Zhao,Xianzi Dong,Feng Jin,Jinfeng Xing,Xuanming Duan
Two-photon polymerization (TPP) microfabrication is an advanced technology to fabricate precise three-dimensional (3D) hydrogel micro/nanostructure. 3D hydrogel microstructures fabricated by TPP with sophisticated details and appropriate stiffness are able to effectively simulate the microenvironment used in tissue engineering and drug delivery. The mechanical property of the microstructures, for instance, the Young’s modulus is crucial to achieve the microstructures with high fidelity. In this study, the mechanical property of the poly(ethylene glycol) (PEG) 3D microstructures fabricated with various laser powers, writing speeds and layer distances in the air was investigated by characterizing the Young’s modulus. Meanwhile, the Young’s modulus of the microstructure with different layer distances in water was determined as 3.50–6.52 MPa. Furthermore, 3D PEG microstructures simulating red blood cell morphology of different postures and sizes were successfully fabricated.
研究领域:
生物材料学
·
组织工程
·
药物递送
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS & DESIGN
·
2022
·
IF 9.417
Zhenyu Yang,Xueqiao Yu,Pengfei Wei,Yiqian Huang,Shuai Zhou,Wei Jing,Yan Zhang,Liya Sun,Guoqiang Bao,Xianli He,Bo Zhao
Pancreatic leakage is severe and life-threatening when happening pancreatitis or conducting pancreatectomy on patients, which easily induces bleeding, infection and even death. Commercial tissue adhesives cannot meet the demands for pancreatic leakage sealing due to the weak adhesiveness, potential toxicity and inability to degrade. Few reports have been feasibly paid attention to pancreas sealants due to the complicated conditions such as enzymatic microenvironments, mechanical dynamics and pre-infections. Herein, we prepared a rapid high-adhesion, antibacterial, biodegradable and injectable bioadhesive based on the FDA-approved polyethylene glycol to seal the pancreatic leakage for clinical translations and commercial applications. The tetra-armed poly(ethylene glycol) succinimidyl glutarate (PEG-SG), tetra-armed poly(ethylene glycol) amine (PEG-NH 2 ) and tannic acid (TA) were integrated to rapidly form the adhesive hydrogel (gelling time < 5 seconds) for convenient operations, while this bioadhesive achieved tough tissue adhesion (∼ 30 kPa), robust antibacterial activity (∼ 95% efficiency) and biocompatibility, revealing the excellent performances for pancreatic wound closure. In the established pre-clinical pancreatectomy using Bama miniature pig model, the bioadhesive presented efficient outcomes on pancreatic leakage, and this indicated the promising feasibility of the bioadhesive for clinical trials and translations.
研究领域:
生物医学工程
·
材料科学
·
外科手术
·
药理学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2016
·
IF 3.902
Wei Zhuang,Linjiao He,Jiahua Zhu,Jianwei Zheng,Xiaojing Liu,Yihui Dong,Jinglan Wu,Jingwei Zhou,Yong Chen,Hanjie Ying
The use of graphene oxide (GO) nanosheets for functional enzyme support has attracted intensive interest owing to their unique planar structure and intriguing physical and chemical properties. However, the detailed effects of the interface properties of GO and its functionalized derivatives on active biomolecules are not well understood. We immobilize nuclease P 1 , a common industrial nucleic acid production enzyme, on pristine and amino poly(ethylene glycol) (PEG-NH 2 ) modified GO nanosheets with interface property heterogeneity using two approaches, physical adsorption and chemical crosslinking. It is demonstrated that nuclease P 1 could be stable immobilized on the surface of pristine GO by physical adsorption and on the edge of modified GO nanosheets by chemical crosslinking. The resultant loading capacity of nuclease P 1 on pristine GO is as high as 6.45 mg/mg as a consequence of strong electrostatic and hydrophobic interactions between the enzyme and carrier. However, it is determined that the acid resistance, thermal stability, reusability and degradation efficiency of the immobilized enzyme on PEG-NH 2 -modified GO are obviously improved compared to those of the enzyme immobilized on pristine GO. The enhanced catalytic behavior demonstrates that GO and its derivatives have great potential in efficient biocatalytic systems.
研究领域:
生物化学
·
纳米生物学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）