MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yudong Xing,Yan Zhou,Peng Liu,Hian Kee Lee,Zhenzhen Huang
A magnetic solid-phase extraction (MSPE) method with synthesized bovine serum albumin-decorated magnetic biochar (BSA/MBC) was developed for the extraction of per- and polyfluoroalkyl substances (PFAS) in food matrices. The combination of the specific affinity of proteins for PFAS and the hydrophobic surface of biochar allowed the material to adsorb a broad spectrum of PFAS without interference from complex matrices. The MSPE-ultrahigh-performance liquid chromatography-Orbitrap high resolution mass spectrometry method was applied to the analysis of 19 target PFAS with salt concentration of NaCl (6 %, w/v), pH of 5, 20 mg of BSA/MBC, 20 min of extraction time, 1 mL of methanol as the elution solvent and 1 min of elution time. The method showed excellent sensitivity with the good linearity in the range of 0.005–10 μg/L, and limits of detection and quantification of 0.001–0.027 μg/L and 0.005–0.091 μg/L, respectively. Furthermore, target PFAS were detected in real samples (i.e., bottled black iced tea, egg, cow’s milk and human milk) with recoveries ranging from 77.3 % to 127 %. In comparison with commercial SPE WAX cartridges, BSA/MBC demonstrated a higher affinity for sulfonamide PFAS.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Di Shen,Haojie Yu,Li Wang,Yu Wang,Yichuan Hong,Chengjiang Li
It was greatly significant, but difficult, to develop stimulus-responsive polymeric nanoparticles with efficient protein-loading and protein-delivering properties. Crucial obstacles were the ambiguous protein/nanoparticle-interacting mechanisms and the corresponding inefficient trial-and-error strategies, which brought large quantities of experiments in design and optimization. In this work, a molecular docking-guided universal “segment-functional group-polymer” process was proposed to simplify the previous laborious experimental step. The insulin-delivering glucose-responsive polymeric nanoparticles for diabetic treatments were taken as the examples. The molecular docking study obtained insights from the insulin/segment interactions. It was then experimentally confirmed in six functional groups for insulin-loading performances of their corresponding polymers. The optimization formulation was further proved effective in blood-glucose stabilization on the diabetic rats under the “three-meal-per-day” mode. It was believed that the molecular docking-guided designing process was promising in the protein-delivering field.
研究领域:
纳米技术
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药学
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生物医学工程
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材料科学
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DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
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2026
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IF 3.6
Congcong Zhao,Zhiqiang Yu,Mengyun Wang,Yingfen Ying,Yunbing Tang,Huitao Li,Ren-shan Ge,Junbo Liang
Per - and polyfluorinated substances (PFAS) are synthetic, long-lasting contaminants that persist in the environment. However, it is still unknown whether these chemicals can inhibit the activity of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) in humans and rats to disrupt glucocorticoid metabolism. Eighteen PFAS (11 carboxylic acids and 7 sulfonic acids) were used to analyze their inhibition on human and rat 11β-HSD1 activity. Eight of the eleven PFAS carboxylic acids (from C7 to C14) gradually inhibited human 11β-HSD1 with the increase of carbon-chain length (inhibitory potency was C4-C6 < C7 < C8 < C9 < C10 < C11 < C12 < C13 < C14). The IC 50 values of perfluoroheptanoic acid (C7) and perfluorotetradecanoic acid (C14) were 100.4 and 10.33 μM, respectively. Perfluorooctanesulfonic acid (PFOS, C8) from 7 sulfonic acids can significantly inhibit human 11β-HSD1. The carbon-chain length, carbon atom number, fluorine atom number, molecular weight, rotatable bonds, boiling point, and melting point, pKa, apolar desolvation, and polar desolvation, and LogP were negatively correlated with IC 50 values to inhibit human 11β-HSD1 (the higher the number, the lower the IC 50 value, thus, stronger inhibition). On the contrary, LogS, Gibbs free energy, and lowest binding energy were positively correlated with IC 50 values. All these chemicals bind to steroid binding site of human 11β-HSD1 as competitive or mixed inhibitors. Only perfluorononanoic acid (C9, IC 50 = 128.2 μM) and perfluorodecanoic acid (C10, 68.21 μM) markedly inhibited rat 11β-HSD1. In conclusion, PFAS show structure-dependent inhibition on human 11β-HSD1 and they increase inhibitory potency with the increase in carbon-chain length, and rat enzyme is less sensitive to the inhibition of these chemicals.
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Yuwen Qi,Huimin Cao,Weijie Pan,Cuiping Wang,Yanna Liang
The negative effects of polyfluoroalkyl substances (PFAS) on the environment and health have recently attracted much attention. This article reviews the influence of soil- and water-derived dissolved organic matter (DOM) on the environmental fate of PFAS. In addition to being co-adsorped with PFAS to increase the adsorption capacity, DOM competes with PFAS for adsorption sites on the surface of the material, thereby reducing the removal rate of PFAS or increasing water solubility, which facilitates desorption of PFAS in the soil. It can quench some active species and inhibit the degradation of PFAS. In contrast, before DOM in water self-degrades, DOM has a greater promoting effect on the degradation of PFAS because DOM can complex with iron, iodine, among others, and act as an electron shuttle to enhance electron transfer . In soil aggregates, DOM can prevent microorganisms from being poisoned by direct exposure to PFAS. In addition, DOM increases the desorption of PFAS in plant root soil, affecting its bioavailability . In general, DOM plays a bidirectional role in adsorption, degradation, and plant uptake of PFAS, which depends on the types and functional groups of DOM. It is necessary to enhance the positive role of DOM in reducing the environmental risks posed by PFAS. In future, attention should be paid to the DOM-induced reduction of PFAS and development of a green and efficient continuous defluorination technology .
研究领域:
环境科学
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生态学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2020
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IF 4.049
Xiaowei Sun,Wenhua Ji,Shenghuai Hou,Xiao Wang
Trifluoromethyl covalent organic framework (CF 3 -COF) is facilely synthesized at room temperature using for solid-phase microextraction (SPME). The CF 3 -COF coating displays an improved extraction performance for per- and polyfluorinated alkyl substances (PFASs) comparing to amorphous polymer coating, non-contained fluorine covalent organic framework coating and commercially available SPME coating. The fluorine affinity of trifluoromethyl in CF 3 -COF for PFASs is demonstrated using the density functional theory calculation. Following the microextraction with CF 3 -COF-coated fiber, the targeted PFASs are eluted with 1 mL acetonitrile and quantified by ultrahigh performance liquid chromatography tandem mass spectrometry. The low limits of detection are 0.1–0.7 pg g −1 for milk and 0.2–0.8 pg g −1 for milk powder, suggesting the outstanding sensitivity. The precision for the method ranges between 2.9 and 9.9%. The fiber-to-fiber repeatability is in the range of 4.2–9.5%. Under the optimum conditions, the relative recoveries are 91.0–110% with RSDs ≤ 10% for milk and 89.8–111% with RSDs ≤ 10% for milk powder.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Liang Li,Wanting Gao,Xinyan Chen,Meijia Liu,Wei Long,Qunyin Zhu
NH₂-MCOF enables ultra-sensitive detection of 17 PFASs down to 0.0346 ng/L. • Fast MSPE within 40 min and reusable for ≥6 cycles with stable performance. • Successfully detected PFASs in real urban waters, including 3.346 μg/L PFDS. Per - and polyfluoroalkyl substances (PFAS), as a class of persistent organic pollutants, are characterized by strong environmental persistence and high bioaccumulation potential, making them a major concern in global environmental and health fields. Currently, the trace detection of PFASs in urban environmental water samples, including surface water, source water, and domestic wastewater faces challenges such as insufficient adsorption selectivity of traditional materials and cumbersome pretreatment steps, highlighting the urgent need to develop efficient enrichment and highly sensitive analytical methods. To improve the enrichment efficiency of PFAS, this study designed and synthesized an amino-functionalized magnetic covalent organic framework material (NH₂-MCOF) as an adsorbent. Combined with magnetic solid-phase extraction (MSPE) and liquid chromatography-tandem mass spectrometry, a synchronous analytical method was established for the determination of 17 PFASs in real urban environmental water matrices. Through systematic optimization, the optimal pretreatment conditions were determined as follows: adsorbent dosage of 20 mg, solution pH of 3.0, ultrasonic-assisted adsorption time of 40 min, and a stepwise elution strategy using methanol, ammoniated methanol, and formic acid-modified methanol. Under the optimized conditions, the method demonstrated good linearity within the range of 0.5–200 μg/L, with detection limits of 0.0346–1.30 ng/L. The spiked recoveries ranged from 50% to 138%, and the intra -day and inter -day precision, expressed as relative standard deviations (RSD), were below 13.5% and 14.9%, respectively. Application to real water samples confirmed the method's effectiveness in detecting multiple PFASs, demonstrating its practicality and reliability. The analytical method established in this study integrates functionalized material design, efficient pretreatment, and sensitive detection, providing a reliable approach for the synchronous monitoring of multiple trace PFAS in real urban environmental water matrices and highlighting the potential application of functionalized magnetic covalent organic framework materials in environmental analysis. Download: Download high-res image (103KB) Download: Download full-size image
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高影响因子
ANALYTICAL CHEMISTRY
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2026
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IF 7.3
Xiaoxuan Ren,Hui Geng,Yue Sun,Shengjie Li,Guizheng Zou
Nanoparticles (NPs) are alternative chemiluminescence (CL) luminophores to molecular luminophores and are strongly anticipated to extend molecular luminophore-tagged commercial CL immunoassays (CLIA) with wavebands beyond the eye-visible region and sensitivity beyond the pg/mL level. Herein, a surficial bond-involved repetitive excitation strategy for near-infrared CL with enhanced photons per luminophore is proposed by exploiting l-methionine (l-Met)-capped gold NPs (AuNPs) as luminophores. The surficial Au–S bonds of l-Met@AuNPs can be involved in the procedure of NP core excitation via oxidation, and bring out defect-involved CL around 830 nm. Because there are plenty of Au–S bonds on every AuNP core, l-Met@AuNPs can be repetitively excited over a hundred times and give off greatly enhanced CL photons per luminophore than all molecular CL luminophores. CL of l-Met@AuNPs/(NH4)2S2O8 can directly enable automatic near-infrared CLIA for myoglobin (MYO) determination on in vitro diagnostic instruments, with a limit of detection of 10 fg/mL (S/N = 3), which significantly surpasses the threshold of molecular luminophores. This Au–S bond-triggered AuNP CL strategy not only offers an alternative to molecular CL strategies with improved sensitivity and to II–VI NP CL strategies with fewer toxic concerns, but also indicates that exploiting the surficial bond energy of NPs could be an alternative to designing NPs with unique morphology, structure, and composition in the CL domain.
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jinni Zhang,Hang Su,Yanhui Zhong,Zian Lin,Zongwei Cai
With the increase of alternative per - and polyfluoroalkyl substances (PFASs) across various applications, the environmental and toxicological implications are emerging as critical issues of increasing scientific and public concern. Accordingly, analytical methodologies must be enhanced to accurately detect and evaluate these alternative PFASs. Herein, a cationic guanidine-based covalent organic frameworks (TG-BPY COF) was synthesized via a one-step solvothermal method for the efficient extraction of alternative PFASs. TG-BPY COF demonstrated good chemical stability and reusability. It possessed a specific surface area of 184.66 m 2 /g and exhibited an adsorption capacity of 185.24, 182.41, 160.97, 160.56, and 232.03 mg g −1 toward PFBA, PFBS, PFHxA, PFHxS, and 6:2 Cl-PFESA, respectively. The characterization and density functional theory (DFT) calculation revealed that the binding mechanism between the TG-BPY COF and alternative PFASs involves size effects, hydrophobic interactions, and electrostatic interactions. Under optimal conditions, a simple and highly sensitive method was established for the quantitative analysis of five alternative PFASs by combining dispersive solid-phase extraction with ultrahigh-performance liquid chromatography-tandem mass spectrometry (dSPE-UPLC-MS/MS). The established methods demonstrated excellent linearity with the alternative PFASs concentration range of 0.10 to 500 ng L −1 ( r ≥ 0.9997), along with extremely low limits of detection (0.030–0.044 ng L −1 ), and high enrichment factors (16.2–61.4). Furthermore, the method was applied to detect trace-level alternative PFASs in environmental water samples, and satisfactory recoveries (78.7 %–122.8 %) and relative standard deviations (RSDs ≤8.7 %) were achieved. This work not only provides an application scope for cationic covalent organic frameworks, but also offers a new strategy for the extraction of alternative PFASs in wastewater.
研究领域:
环境科学
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分析化学
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材料科学
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化学工程
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毒理学
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产品关联
DOI
高影响因子
Journal of Environmental Sciences
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2025
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IF 6.3
Xinting Zhao,Qinghua Zhou,Jiaxing Xie,Xin He,Yan Wang,Feng Tan
Soil aging is recognized to influence the soil-water partitioning behavior of organic pollutants, altering their mobility and bioavailability. However, the specific impacts of soil aging on the desorption behavior of per- and polyfluoroalkyl substances (PFAS) from the solid phase require further investigation. This study investigated the impact of soil aging on the distribution and desorption kinetics of eleven legacy and emerging PFAS in agricultural soils, using the diffusive gradients in thin films (DGT) technique. The results demonstrate that soil aging strengthened interactions between PFAS and soil particles, simultaneously reducing concentrations in both the soil solution and solid phase compartments. Additionally, high organic matter content facilitated PFAS sequestration. The nonlinear increase in PFAS mass accumulated by DGT device suggested that the consumption of PFAS at the DGT surface was partially offset by the resupply of labile PFAS from the solid phase. Modeling with the DGT-induced fluxes in soil model indicated that the labile pool size decreased following soil aging, with values ranging from 0.11 to 168 mL/g after 14 days and from 0.34 to 83 mL/g after 120 days. Overall, this study demonstrates that soil aging reduced PFAS concentration in the soil solution while also decreasing the labile pool size. These findings are significant for assessing PFAS transfer, fate, bioavailability, and potential risks to ecosystems and human health.
研究领域:
环境科学
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土壤化学
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污染物归趋与迁移
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分析化学
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产品关联
DOI
高影响因子
ACS ES&T Engineering
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2025
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IF 6.7
Yuwen Qi,Fuyu Li,Chunhui Zhang,Xuejiao Tang,Cuiping Wang,Yanna Liang,Hongwen Sun,Baoshan Xing
Severe harmful effects of per-fluoroalkyl and polyfluoroalkyl substances (PFAS) to ecology and human health have led many countries to continuously upgrade their control for PFAS, which poses higher requirements for the efficient removal of PFAS in water to ensure water resource security. In the present study, the three-dimensional/two-dimensional (3D/2D) structure of metal oxide immobilized on a carbon substrate of the Fe-based metal–organic frameworks (Fe-MOF) heat treatment derivative H:MIL-101(Fe) was constructed. H:MIL-101(Fe) could completely remove PFOA from pure water within 5 min, and complete recovery was achieved within 30 s. H:MIL-101(Fe) exhibited stable properties and can maintain its initial adsorption percentage after four cycles of PFAS adsorption. It was found that the high hydrophobic interface of H:MIL-101(Fe) was formed by partial carbonization during the Fe-MOF heat treatment, and a large number of unsaturated ═C–H bonds on the unstructured benzene ring were generated. Furthermore, cationic chelating agent, such as cetyltrimethylammonium bromide, were mixed with PFAS to synthesize more hydrophobic long-chain complexes; six PFAS containing 4–8 carbon atoms could be completely coadsorbed in surface water within 10 min at pH 7.0, mainly depending on the mechanism of hydrophobic interaction. This study develops a novel adsorption material that can match PFAS types to realize the effective coadsorption of PFAS in water, which provides new insights for removal of PFAS with different carbon chains based on improving hydrophobic interactions between adsorbents and adsorbates.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Xiang Wang,Xingrui Wang,Hanqi Yang,Hanlin Zeng,Zhiqiang Xu,Wenhao Chen,Guangming Zhou,Jingdong Peng
In this study, we synthesized and employed an ionic gel-functionalized silica stationary phase for high-performance liquid chromatography. The successful fabrication of the stationary phase was confirmed through attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), zeta-potential measurements, and elemental analysis (EA). Comparative performance evaluation against a commercial column demonstrated the prepared column's effectiveness in the mixed mode of reversed-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), and ion chromatography (IC). Moreover, the stationary phase exhibited exceptional retention repeatability in per aqueous liquid chromatography, showcasing its potential as an environmentally friendly analytical method. Mechanistic investigations unveiled multiple solute-stationary phase interactions, including π-π interactions, hydrogen bonding, and ion exchange. Finally, we applied the developed stationary phase for the precise detection of preservatives in carbonated beverages and jelly, achieving high levels of accuracy and recovery rates.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Chao Wen,Huan Chen,Yunbing Tang,Hang Lin,Congcong Xu,Yingfen Ying,Yang Zhu,Xinjun Miao,Ren-shan Ge,Chao Chen,Shangqin Chen
Per- and polyfluoroalkyl (PFAS) substances are enduring industrial materials. 17β-Hydroxysteroid dehydrogenase isoform 1 (17β-HSD1) is an estrogen metabolizing enzyme, which transforms estrone into estradiol in human placenta and rat ovary. Whether PFAS inhibit 17β-HSD1 and what the structure-activity relationship (SAR) remains unexplored. We screened 18 PFAS for inhibiting human and rat 17β-HSD1 in microsomes and studied their SAR and mode of action(MOA). Of the 11 perfluorocarboxylic acids (PFCAs), C8-C14 PFCAs at a concentration of 100 μM substantially inhibited human 17β-HSD1, with order of C11 (half-maximal inhibition concentration, IC 50 , 8.94 μM) > C10 (10.52 μM) > C12 (14.90 μM) > C13 (30.97 μM) > C9 (43.20 μM) > C14 (44.83 μM) > C8 (73.38 μM) > others. Of the 7 per- and poly-fluorosulfonic acids (PFSAs), the potency was C8S (IC 50 , 14.93 μM) > C7S (80.70 μM) > C6S (177.80 μM) > others. Of the PFCAs, C8-C14 PFCAs at 100 μM markedly reduced rat 17β-HSD1 activity, with order of C11 (IC 50 , 9.11 μM) > C12 (14.30 μM) > C10 (18.24 μM) > C13 (25.61 μM) > C9 (67.96 μM) > C8 (204.39 μM) > others. Of the PFSAs, the potency was C8S (IC 50 , 37.19 μM) > C7S (49.38 μM) > others. In contrast to PFOS (C6S), the partially fluorinated compound 6:2 FTS with an equivalent number of carbon atoms demonstrated no inhibition of human and rat 17β-HSD1 activity at a concentration of 100 μM. The inhibition of human and rat enzymes by PFAS followed a V-shaped trend from C4 to C14, with a nadir at C11. Moreover, human 17β-HSD1 was more sensitive than rat enzyme. PFAS inhibited human and rat 17β-HSD1 in a mixed mode. Docking analysis revealed that they bind to the NADPH and steroid binding site of both 17β-HSD1 enzymes. The 3D quantitative SAR (3D-QSAR) showed that hydrophobic region, hydrogen bond acceptor and donor are key factors in binding to 17β-HSD1 active sites. In conclusion, PFAS exhibit inhibitory effects on human and rat 17β-HSD1 depending on factors such as carbon chain length, degree of fluorination, and the presence of carboxylic acid or sulfonic acid groups, with a notable V-shaped shift observed at C11.
研究领域:
环境毒理学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）