ELECTROCHEMISTRY
·
2024
·Vol. 92
·期 2
·027006-027006
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IF 2.7
Bingkai HAN,Yuan CHEN,Haotian WANG,Wenying ZHAO,Yuhan HU,Yayun GUO,Jilong YAN,Shixiong JIA
Prostate specific antigen (PSA), as a biomarker, plays important roles in early diagnosis of male prostate diseases, especially prostate cancer. In this study, a novel electrochemical probe polymer of poly(ferriporphyrin-co-acrylamide) was synthesized, by further combined with reduced graphene oxide and gold nanoparticles, and oriented immobilization of PSA antibody, a label-free electrochemical immunosensor was obtained, and then successfully applied in the determination of PSA in 10 % non-antigen serum system. The prepared biosensor expressed high selectivity toward PSA, with a LOD of 0.001 ng mL−1, a linear range of 0.01–110 ng mL−1, and a sensitivity of 15.78 µA mL ng−1. What’s more, it showed good stability, well repeatability and high selectivity in the real PSA sample detection. This work provided certain references for the design of the new electrochemical probes and the detection of tumor markers.
研究领域:
生物传感器
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纳米技术
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电化学
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癌症诊断
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jian Wang,Fangming Chen,Qiaoyu Yang,Yao Meng,Mingrui Jiang,Yaqiong Wang,De-Wen Zhang,Liping Du
Multi-channel evaluation on a single sensor interface is highly valuable but full of challenge. Herein, a universal light-addressable electrochemical immunoassay based on silicon photoelectrodes was proposed for spatially-resolved detection of prostate-specific antigen (PSA) as a model target. To stabilize and functionalize silicon substrate, a simple and robust antifouling coating was prepared by cross-linking bovine serum albumin (BSA) matrix with glutaraldehyde (GA). For the PSA detection, an electrochemical sandwich-type immunosensor was performed on the basis of [email protected] functionalized silicon, in which horse radish peroxidase (HRP) was used to catalyze the reaction of H 2 O 2 and hydroquinone (HQ), resulting in in situ generation of benzoquinone (BQ). The light-induced local reduction photocurrent of BQ was then recorded as the sensor response by irradiating the target spot of photoelectrode respectively with a moveable light pointer. The results demonstrated the feasibility of localized measurements of enzymatic reactions . Coupling with light-addressable electrochemistry (LAE) and enzyme-linked immunosorbent assay (ELISA) protocol, multiplexed PSA detection could be achieved on a single sensor chip with good sensitivity and specificity. This proof-of-concept study provides a promising platform for high-throughput and accurate immunoanalysis in diagnostic or biodefense applications.
研究领域:
生物化学
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分析化学
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生物医学工程
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免疫学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Wenwei Pan,Ziyu Han,Ye Chang,Xuexin Duan
We have developed a new three-dimensional (3D) surface for use in biosensors that is based on modified novel thorns-like polyelectrolytes (3D-PETx), which comprises of poly- L -lysine (PLL) appended with multitude oligo (ethylene glycol) (OEG) and biotin moieties. It tethered to the sensor surface by PLL, while the OEG-biotin chains are forced to stretch away from the surface for target detections. Due to its 3D structure, the number of the OEG-biotin per surface unit is markedly increased compared to conventional 2D polyelectrolytes (2D-PET) coating. Their antifouling property and sensing performance for human IgG and PSA were compared with the 2D-PET by BioLayer Interferometry (Blitz), Surface Plasmon Resonance (SPR), microfluidic devices and Enzyme-Linked ImmunoSorbent Assay (ELISA). Experimental results show that 3D-PETx presents higher sensitivity for biomarker detection both in buffer and in serum and provides an almost non-fouling surface even in undiluted serum. In addition, a sensitive PSA detection was achieved in undiluted serum with a LOD down to 0.6 ng/mL. The successful immunosensing in undiluted serum demonstrate the potential of the 3D-PETx coating for real clinical applications.
研究领域:
生物传感器
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聚合物化学
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分析化学
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生物技术
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Yuan-Cheng Zhu,Fei Xu,Nan Zhang,Wei-Wei Zhao,Jing-Juan Xu,Hong-Yuan Chen
This work reports the use of compositionally heterogeneous asymmetric [email protected] core–satellite nanoassembly functionalized with DNA sequence as unique signaling nanoprobes for the realization of new energy-transfer-based photoelectrochemical (PEC) immunoassay of prostate- specific antigen (PSA). Specifically, the [email protected] asymmetric core–satellite nanoassemblies ( [email protected] ACS) were fabricated on a two-dimensional glass substrate by a modified controlled assembly technique, and then functionalized with DNA sequences containing PSA aptamers as signaling nanoprobes. Then, the sandwich complexing between the PSA, its antibodies, and the signaling nanoprobes was performed on a CdS QDs modified indium tin oxide (ITO) electrode. The single stranded DNA can server as a facile mediator that place the [email protected] ACS in proximity of CdS QDs, stimulating the interparticle exciton–plasmon interactions between [email protected] ACS and CdS QDs and thus quenching the excitonic states in the latter. Since the damping effect is closely related to the target concentration, a novel energy-transfer-based PEC bioanalysis could be achieved for the sensitive and specific PSA assay. The developed biosensor displayed a linear range from 1.0×10 −11 g mL −1 to 1.0×10 −7 g mL −1 and the detection limit was experimentally found to be of 0.3×10 –13 g mL −1 . This strategy used the [email protected] ACS-DNA signaling nanoprobes and overcame the deficiency of short operating distance of the energy transfer process for feasible PEC immunoassay. More significantly, it provided a way to couple the plasmonic properties of the Ag NPs and Au NPs in a single PEC bioanalytical system. We expected this work could inspire more interests and further investigations on the advanced engineering of the core–satellite or other judiciously designed nanostructures for new PEC bioanalytical uses with novel properties.
研究领域:
纳米技术
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生物化学
·
分析化学
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生物物理学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Xin Liu,Dan Wang,Jinshen Chu,Yang Xu,Wenjun Wang
Prostate-Specific Antigen (PSA) is a crucial biomarker for screening prostate cancer, but a sensitive and selective immunosensor for rapid quantification of serum PSA remains to be developed. In this study, a sandwich pair of nanobodies (Nbs) (i.e., Nb2 and Nb40) against PSA surface antigen was obtained from an alpaca-derived immune phage display library. A sandwich-type immunosensor for the sensitive and selective detection of PSA in serum samples was ingeniously designed based on the pair of Nbs. The small size of Nb40 allowed high capture densities on the surface of reduced graphene oxide (rGO) nanocomposed with massive Au nanoparticles ( [email protected] ), which significantly improved the conductivity and provided a large area to anchor many primary antibodies. The secondary antibody Nb2 fused with streptavidin -binding peptide (SBP) cooperated with Nb40 for PSA sandwiching. Accompanying introduction of horseradish peroxidase-streptavidin (HRP-SA) coupled with Nb2-SBP, the faradaic current was linearly correlated with the logarithm of PSA concentration in a range of 0.1–100 ng mL −1 . More importantly, this immunosensor exhibited excellent selectivity, stability, and reproducibility due to the sandwich pair Nbs. The proposed immunosensor was successfully applied in determining PSA in serum samples and could be used for the sensitive and specific detection of PSA.
研究领域:
生物技术
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纳米技术
·
免疫学
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癌症研究
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xiaoqing Zhao,Jing Wang,Hou Chen,Hui Xu,Liangjiu Bai,Wenxiang Wang,Huawei Yang,Donglei Wei,Baiqing Yuan
A novel electrochemical immunoassay platform for multi-signal amplification has been constructed and applied to the simultaneous detection of three tumor markers (TMs). The platform was successfully developed by polyethyleneimine (PEI)-polydopamine (PDA)/reduced graphene oxide (rGO) nanocomposites. PDA/rGO as the substrates can simultaneously load with detection antibodies (Ab 2 ) and large amounts of PEI, which PEI were further used to immobilize different signal labels. Three TMs of carcinoembryonic antigen (CEA), prostate specific antigen (PSA) and alpha fetoprotein (AFP) were employed as potential factors. Ferrocenecarboxylic acid (Fc), anthraquinone-2-carboxylic acid (Aq) and acetylsalicylic acid (ASA) were employed as distinguishable signal labels. Electrochemical responses of designed sandwich-type immunosensor were successfully investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The multiplexed immunoassay displayed excellent linear response in the range of 0.102 pg/mL–112 ng/mL for CEA and PSA, 0.117 pg/mL–117 ng/mL for AFP. The detection limit of three TMs were 91 fg/mL, 101 fg/mL and 83 fg/mL for CEA, PSA and AFP, respectively. Moreover, this electrochemical immunoassay platform exhibited excellent reproducibility, sensitivity and selectivity for the simultaneous detection of multiple biomarkers.
研究领域:
生物医学工程
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分析化学
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纳米技术
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电化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
·
2023
·
IF 3.4
Cheng Fang,Chaobo Wu,Xiaoyan Zhao
Cross-linked acrylate latex pressure sensitive adhesives (PSAs) were synthesized via unseeded semi-batch emulsion polymerization processes with butyl acrylate (BA), acrylic acid (AA), 2-hydroxyethyl acrylate (HEA) and difunctional cross-linker, ethylene glycol dimethacrylate (EGDMA). The influences of EGDMA on the comprehensive properties of the acrylate latex PSAs were investigated. The results indicated that both viscosity and particle size of the latexes were independent of the amount of EGDMA in the pre-emulsion feed when unseeded semi-batch emulsion polymerization process was employed. It was also found that water resistance of the latex PSA was improved with increasing EGDMA content, further confirmed by water contact angle measurements . DSC and TGA results showed that T g of the copolymer was elevated, plus thermal stability was increased with introduction of EGDMA. Besides, with the increase of EGDMA content, the gel content of the polymer increased. Moreover, for the cross-linked adhesive film , the shear strength was improved greatly while at the sacrifice of loop tack and peel strength, when compared with the uncross-linked counterparts.
研究领域:
材料科学
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聚合物化学
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粘合技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Ning Xia,Gang Liu,Shuo Zhang,Zichen Shang,Yinpei Yang,Yuanyuan Li,Lin Liu
This work reported that the peptide-Cu 2+ complexes reveal oxidase-mimicking activities for oxygen reduction and ascorbate oxidation . The peptide features a histidine (His) residue in the first position with respect to the free N-terminal amine. The complexes can be easily integrated with nanomaterials such as gold nanoparticles (AuNPs) to form nanocatalysts or nanozymes, which allows for the fabrication of sensitive sensing platforms. To explore the analytical performances of the peptide-Cu 2+ complexes in biosensing, sandwich electrochemical and fluorescent immunoassays of prostate specific antigen (PSA) were performed with AuNP/peptide-Cu 2+ conjugates as the nanolabels. In the electrochemical immunoassays, the nanolabels coined as nanocatalysts promoted the electrocatalytic reduction of oxygen. For the fluorescent assays of PSA, the nanolabels coined as nanozymes catalyzed the oxidation of ascorbic acid (AA), and the resulting oxidization product dehydroascorbic acid (DHA) was reacted with o-phenylenediamine (OPD) to form fluorescent 3-(dihydroxyethyl)furo [3,4-b]quinoxaline-1-one (DFQ). The electrochemical and fluorescent signals are proportional to the concentration of PSA in the linear range of 0.001–0.50 and 0.001–2.00 ng/mL, respectively. The low detection limits of the methods (0.40 and 1.00 pg/mL) enabled clinical detection of PSA in serum samples. The findings should be valuable for understanding the biological functions of peptide-Cu 2+ complexes, and provide new insight to develop optical and electrochemical sensing platforms for clinical diagnosis.
研究领域:
生物化学
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分析化学
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生物传感器
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临床诊断
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电化学
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荧光分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 12
·2266
·
IF 3.06
Xueying Liu,Xiaoku Ran,Muhammad Riaz,Haixue Kuang,Deqiang Dou,Decheng Cai
The major objective of this study was to investigate the anti-chronic nonbacterial prostatitis (CNP) mechanism ofT. patulaby metabolomics and network pharmacology. The study demonstrated that the flavonoids and polysaccharides ofT. patulacould alleviate prostatitis by improving the level of DHT, reducing the secretion of PSA and TNF-α. Besides, both could enhance Na+/K+-ATPase activity, decrease the O2consumption, CO2production, heat production, energy expenditure of rats and promote respiratory exchange ratio of rats. Up to 28 potential biomarkers and 8 key metabolic pathways related to the treatment of CNP were elucidated by the metabolomics analysis, including phenylalanine metabolism, taurine and hypotaurine metabolism, tryptophan metabolism etc. Network pharmacology prediction also reflected the potential mechanism was associated with tryptophan metabolism and energy pathway. Generally, the potential anti-CNP mechanism of flavonoids and polysaccharides ofT. patulamight be through reducing the expression of inflammation factors, adjusting the level of hormone and regulating the amino acid metabolism, energy metabolism and glucose and lipid metabolism.Keywords:Tagetes patulaL.;chronic nonbacterial prostatitis;metabolomics;energy metabolism;network pharmacology
研究领域:
药理学
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代谢组学
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传统医学
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前列腺疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）