ACS Applied Nano Materials
·
2024
·
IF 5.3
Kun Wang,Yingru Tang,Kangli Xing,Xiaoming Mou,Xiaoyan Yang,Zhenlan Quan
Protein self-assembly has attracted extensive interest in the field of biomaterials and biomedicine due to its inherent biocompatibility and flexible designability. In this paper, we propose a potential route to generate a photoelectrochemical (PEC) sensing platform through Sn-triggered protein self-assembly. Specifically, Sn2+ ion was used to trigger the self-assembly of bovine serum albumin (BSA) to form in situ Sn/BSA film on the indium tin oxide (ITO) glass. Then, a CdS thin film as a PEC active material was electrodeposited on the Sn/BSA modified ITO electrode. The hybrid Sn/BSA film was demonstrated to stabilize the PEC response of the CdS. A dual-electrode PEC cell comprised a graphene oxide (GO)-modified photocathode and a Sn/BSA hybrid film/CdS-loaded photoanode. Pb2+-response deoxyribozymes (DNAzymes) were hybridized with the substrate strands tagged with Au nanoparticles (Au NPs) and then immobilized on the photocathode. Upon Pb2+-induced cleavage, Au NP-linked oligonucleotide fragment dislocated from the photocathode surface, resulting in a decrease in the PEC response. The present PEC biosensor showed highly sensitive detection of Pb2+ with a wide detection range from 10.0 fM to 0.1 μM and a low detection limit of 4.0 fM. This work demonstrates that the Sn/BSA hybrid film has potential application prospects in PEC sensing, thereby providing a new way of thinking for the design and development of the bioinspired biosensor.
研究领域:
生物材料
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生物医学
·
分析化学
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 18
·4346-4355
·
IF 4.2
Jiahai Wang,Cenlin Gui,Jianji Zhu,Baian Zhu,Zhuobin Zhu,Xiwen Jiang,Daqi Chen
Glass nanopore is an ideal candidate for biosensors due to its unique advantages such as label-free analysis, single-molecule sensitivity, and easy operation. Previous studies have shown that glass nanopores can distinguish different lengths of double-stranded DNA (dsDNA) at the same time with the length-resolution ability. Based on this, we proposed a novel design of a dsDNA block containing a programmable sensing site inside, which can be programmed to respond to different target molecules and cleaved into two smaller DNA blocks. When programming the sensing site with different sequences, for example, programming it as the substrate of GR-5 DNAzyme and CRISPR-Cas12a system, the DNA block could realize Pb2+ and cfDNA detection with the length-resolution ability of the glass nanopore. This strategy achieved a Pb2+ detection range from 0.5 nM to 100 nM, with a detection limit of 0.4 nM, and a BRCA-1 detection range from 1 pM to 10 pM, with a detection limit of 1 pM. The programable sensing site is easy to design and has strong expandability, which gives full play to the advantages of glass nanopore in length-resolution ability for dsDNA, and is expected to become an optional design for biosensing strategy for the glass nanopore as a biosensing platform.
研究领域:
生物传感器
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分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Chunhong Zhu,Wanying Zhu,Lei Xu,Xuemin Zhou
Herein, a novel magnetic biocomposite (Fe 3 O 4 @Au-S1/S2) was applied to analyze thrombin. The Fe 3 O 4 @Au-S1/S2 consisted of Fe 3 O 4 @Au nanoparticles (Fe 3 O 4 @Au NPs) as carriers for magnetic separation and magnetic field-induced self-assembly, thiolated complementary strand (S1) anchored based on Au-S bond and thrombin binding aptamer (S2) as a recognition element. As a redox indicator , methylene blue (MB) can be adsorbed to DNA anchored on the surface of Fe 3 O 4 @Au NPs by electro-static interaction. In the absence of thrombin, MB were adsorbed on double-stranded DNA (S1/S2) which anchored on Fe 3 O 4 @Au NPs and a high electrochemical signal of MB was recorded by Differential pulse voltammetry . Conversely, the complementary strand (S1) exposed after thrombin competitively bonded with aptamer. The introduction of Pb 2+ -dependent DNAzyme (S3) split S1 at specific rA site, resulting in the significantly decreased adsorption capacity of MB. Thus, the thrombin detection could be recorded by monitoring the electrochemical signal reduction of MB through incubation of thrombin with S3. This method exhibited a high sensitivity toward thrombin with a broad linear range from 5 pmol L −1 to 5 nmol L −1 and a limit of detection of 1.8 pmol L −1 .
研究领域:
分析化学
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生物传感器
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电化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Xiao-Fan Lu,Wen-Hua Ji,Lin Yuan,Shui Yu,Dian-Shun Guo
A carboxy-functionalized covalent organic framework COOH@COF for efficient removal of Pb2+ and Hg2+ ions from water has been prepared. Fourier transform infrared spectroscopy, solid-state 13C NMR spectra, and elemental analysis indicate that the carboxy function has been grafted to COF materials via the click reaction. Its crystallinity remains unchanged after post-synthetic modification, as confirmed by the powder X-ray diffraction. Scanning electron microscopy images reveal that the COOH@COF possesses the thorn ball structure assembled by numerous nanowires with a diameter of 90 nm. This COOH@COF features excellent adsorption capacities for Pb2+ (123.8) and Hg2+ (99.1 mg/g) in water. The adsorption isotherms follow the Langmuir model, and their kinetics adsorption fits the pseudo-second-order model. Particularly, the COOH@COF can be recycled 20 times without loss of the adsorption capacity in water, showing its latent potential for practical application in the future.
研究领域:
环境科学
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材料科学
·
水处理
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Jun Wan,You Shen,Liqiang Xu,Ruixue Xu,Jinfan Zhang,Haodi Sun,Chongyu Zhang,Chuanxia Yin,Xinxing Wang
The development of selective electrochemical response materials and new detection strategies are the research focus in the field of heavy metal ion (HMI) electroanalysis. Herein, a Ni(II)-based metal-organic framework (NH 2 -Ni-MOF) was synthesized and functionalized by electrochemically active molecule of ferrocene (Fc) via post-synthesis modification. Based on the Fc-functionalized NH 2 -Ni-MOF (Fc-NH 2 -Ni-MOF), a novel electrochemical ratiometric sensing platform was developed and applied for the simultaneous determination of various HMIs. The prepared NH 2 -Ni-MOF exhibits nanoplate structure, which is conducive to increase accessible electrode area and expose more active sites, thus promoting the adsorption and pre-concentration of HMIs. The modification of Fc on MOF not only enhances the electrical conductivity of the MOF material, but also provides an internal reference signal for the ratiometric analysis. Due to the excellent characteristics of the Fc-NH 2 -Ni-MOF, the as-prepared ratiometric electrochemical sensing platform exhibits wide linear ranges (0.001 μM to 2.0 μM for lead ions (Pb 2+ ), and 0.01 μM to 2.0 μM for copper ions (Cu 2+ ) as well as cadmium ions (Cd 2+ )) and high sensitivity (the detection limit toward Cu 2+ , Pb 2+ and Cd 2+ is 6.3 nM, 0.2 nM and 7.1 nM, respectively). In addition, compared with the non-ratiometric strategy, the reproducibility of ratiometric analysis is significantly improved. The developed electrochemical method with high sensitivity, selectivity and reliability exhibits bright application prospects in the detection of real samples. Moreover, the electrochemical application of MOF materials has been effectively expanded.
研究领域:
电化学
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分析化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Hai-Bo Wang,Li-Hong Ma,Bi-Yun Fang,Fang Tan,Yuan-Cheng Cao,Yuan-Di Zhao,Xue-Bin Hu
PS2M aptamer was selected as sensing element to construct Pb 2+ strip biosensor, and for the first time, AuNP-DNA polymer was used as sensitivity enhancement probe (SEP) to improve sensitivity. Pb 2+ separated the double-strand formed by PS2M and its complementary sequence at Anti-PS2M and induced PS2M to form G-quadruplex structure, then single-strand Anti-PS2M was released. When migrated along the strip, sequence at one end of Anti-PS2M hybridized with AuNP-DNA probe G immobilized on conjugate pad, when the composite migrated to test zone (TZ), other end of Anti-PS2M hybridized with biotin-DNA probe T on TZ to form sandwich structure. Subsequent SEP hybridized with the remaining oligonucleotides of AuNP-DNA probe G on TZ thus enhanced the signal. After optimization, the false positive result could be eliminated and the best signal value could be obtained. The results showed that the limit of detection of Pb 2+ strip biosensor was 2.5 nM by adding the sensitivity enhancement probe, which was 40 times better than that strip without enhancement. The test strip showed good results in the direct detection of soil and lake samples, presenting good practical value. In addition, the cost of this strip biosensor was low, with good market prospect.
研究领域:
分析化学
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环境科学
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生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Materials
·
2022
·
IF 3.985
Chen Yu,Liu Miao
Application of amino-functionalized mesoporous silica-coated core-shell magnetic nanoparticles (Fe3O4@mSiO2-NH2 NPs) for adsorbing heavy metal ions has attracted intensive interest in recent years. Despite the cytotoxicity triggered by the co-exposure of nanoparticles (NPs) and metal ions in relatively high dosages being reported, the effect of the adsorbed heavy metal ions on the cytotoxicity to human cells remains unexplored. Herein, we demonstrated the effect of amino-functionalized Fe3O4@mSiO2 core-shell magnetic nanoparticles before and after adsorbing Pb2+ ions on the cytotoxicity of human kidney cells (HEK293). The surface morphology, viability, and oxidative stress (OS) induction of HEK293 cells incubated with Fe3O4@mSiO2-NH2 NPs and Pb2+ ion-adsorbed Fe3O4@mSiO2-NH2 NPs were assessed, respectively. Transmission electron microscopic (TEM) images of cell sections depicted that Fe3O4@mSiO2-NH2 NPs were internalized by HEK293 cells and gathered mainly in the cytoplasm. Cell viability (MTT) assays revealed the Fe3O4@mSiO2-NH2 NPs could enhance the cell viability to 119.9% and 108.2% compared to the control group, respectively. On contrast, the Pb2+ ion-adsorbed Fe3O4@mSiO2-NH2 NPs were toxic to the cell because when the Pb2+ ion contents were 5.0 and 7.5 μg mL−1, the viabilities of the samples decreased to 97.1% and 84.7%, respectively. Oxidative stress data proved that OS was negatively affected by both dissociative Pb2+ ions and the Pb2+ ion-adsorbed Fe3O4@mSiO2-NH2 NPs. Cytotoxicity may be attributed to the OS induced by Pb2+ ions leaked from the adsorbent. Under the same Pb2+ ion concentration, the cytotoxicity of the adsorbed Pb2+ ions was lower than that of the dissociative Pb2+ ions, indicating that the adsorption by NPs inhibited the cytotoxicity of Pb2+ ions. This work will provide new references for assessing the cytotoxicity of Pb2+-adsorbed nanoparticles.
研究领域:
纳米技术
·
毒理学
·
细胞生物学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Yuru Yang,Lei Zeng,Zongkun Lin,Huabin Jiang,Aiping Zhang
A chitosan-based bead was synthesized by crosslinking as well as sulfhydryl modification reaction and its removal ability of Pb 2+ , Cu 2+ and Cd 2+ was investigated. The test results showed that the crystal structure of chitosan was destroyed completely and the specific surface area was greatly increased after modification. The adsorption of Pb 2+, Cu2+ and Cd2+ by the beads was carried out at different pH, ionic strength , contact time and initial concentration and the maximum adsorption capacities were 273.7 mg/g, 163.3 mg/g and 183.1 mg/g, respectively. Furthermore, due to the large ion radius of Pb 2+ , its adsorption was seriously disturbed by other ions in the competitive adsorption process. Finally, the adsorption processes of Pb 2+ , Cu 2+ and Cd 2+ were well fitted by the Langmuir isotherm model and the pseudo second-order kinetics model , respectively. Combined with the results of X-ray photoelectron spectroscopy, chemical coordination is the main adsorption mechanism.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2023
·
IF 8.6
Meng Gao,Yuexiao Pan,Chengdong Peng,Yihong Ding,Hongzhou Lian,Liyi Li,Jun Lin
Single-component white light emitters are particularly attractive for fabricating economical solid-state devices for display and lighting. Herein, an efficient white light with photoluminescence quantum yields approaching 98% has been obtained in Mn2+ and Pb2+ co-doped Cs7Cd3Br13 crystals. The quantified bonding and nonbonding characters calculated by the crystal orbital Hamilton population analysis demonstrate that the Mn2+ and Pb2+ occupy preferentially in the [CdBr4]2– tetrahedrons rather than the [CdBr6]4– octahedrons in Cs7Cd3Br13. According to the PL spectral characteristic and the theoretical results, the broadband white light emission from Cs7Cd3Br13:Pb2+,Mn2+ crystals is ascribed to the multiple PL origins, i.e., the first self-trapped exciton (STE1) from the intrinsic trapping states of host lattice Cs7Cd3Br13, the d–d transition of Mn2+, and the second self-trapped exciton (STE2) induced by the introduction of Pb2+, respectively. The results calculated by density functional theory reveal that the incorporation of Mn2+ and Pb2+ has significantly improved the thermodynamic stability of the Cs7Cd3Br13 structure with lower defect formation energies, which is verified by the experimental observation in the temperature-dependent PL spectra of the emitting crystals. The findings here provide a new perspective for a single-component white light via creating multiple PL centers in a single matrix as well as help in the elucidation of the preferential site occupancy mechanism of the dopants in different symmetrical units.
研究领域:
材料科学
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光学
·
固体物理
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 10
·766
·
IF 5.3
Ning Wang,Ming He,Longjiao Wang,Chuanjie Lei,Linyufan Xiao,Yingjie Li,Shuan Liu
An optical fiber sensor based on a HEMA/AM/SA interpenetrating polymer network (IPN) hydrogel is proposed for monitoring the concentration of Pb2+. The Fabry–Perot interference cavity is constructed from a single-mode fiber, a ceramic ferrule, and an IPN hydrogel layer. P (HEMA co AM)/SA IPN hydrogel films were prepared by a step-by-step crosslinking method, which had good mechanical properties, swelling properties, and Pb2+adsorption capacity. The Pb2+concentration changes cause the interference spectrum shift of the sensor. By monitoring the wavelength shift under different Pb2+concentrations, the sensor sensitivity in the range of 0~1 ppm Pb2+concentration in solution is 5.0743 nm/ppm with 0.994 linearity. The influence of different proportions of IPN hydrogel on the performance of the sensor was studied. In the range of 10–90% HEMA, higher sensitivity is obtained by a small weight ratio of HEMA/AM. The sensor stability, repeatability, selectivity, dynamic response, and temperature response are also investigated in experiments. Experimental results demonstrate that the proposed sensor exhibits good stability, sensitivity, repeatability, and selectivity. Owing to its compact structure, straightforward fabrication, low cost, and good sensing performance, this sensor shows strong potential for application in monitoring Pb2+concentrations.
研究领域:
生物医学工程
·
环境监测
·
传感器技术
·
功能材料
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2022
·
IF 3.898
Guangyuan Chen,Tong Zhou,Meng Zhang,Zhongxiang Ding,Zhikun Zhou,Yuanhui Ji,Haiying Tang,Changsong Wang
Struvite (MAP) crystallization technology is widely used to treat ammonia nitrogen in waste effluents of its simple operation and good removal efficiency. However, the presence of heavy metal ions in the waste effluents causes problems such as slow crystallization rate and small crystal size, limiting the recovery rate and economic value of the MAP. The present study was conducted to investigate the effects of concentrations of three heavy metal ions (Cu 2+ , Zn 2+ , and Pb 2+ ) on the crystal morphology, crystal size, average growth rate, and crystallization kinetics of MAP. A relationship was established between the kinetic rate constant K t calculated by the chemical gradient model and the concentrations of heavy metal ions. The results showed that low concentrations of heavy metal ions in the solution created pits on the MAP surface, and high level of heavy metal ions generated flocs on the MAP surface, which were composed of metal hydroxides, thus inhibiting crystal growth. The crystal size, average growth rate, MAP crystallization rate, and kinetic rate constant K t decreased with the increase in heavy metal ion concentration. Moreover, the K t demonstrated a linear relationship with the heavy metal concentration ln( C / C* ), which provided a reference for the optimization of the MAP crystallization process in the presence of heavy metal ions.
研究领域:
环境科学
·
化学工程
·
水处理
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产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 4
·1395-1400
·
IF 1.938
Li Wang,Di Luo,Dongdong Qin,Duoliang Shan,Xiaoquan Lu
This paper reports the electrochemiluminescence (ECL) behavior of CdSe/ZnS with K2S2O8 as the coreactant. The effects of pH, K2S2O8 concentration, the duration of the reaction of 4-ATP with GCE and the duration of the reaction of 4-ATP/GCE with QDs on ECL intensity were studied in detail. A possible ECL reaction mechanism was then proposed. In addition, it was observed that the ECL intensity was efficiently quenched by trace amounts of heavy metal ion. Based on the quenching effect of Pb2+ upon the ECL of CdSe/ZnS QDs, a QDs-ECL sensor was constructed to detect Pb2+ in preserved duck egg. The sensor showed good reproducibility and stability.
研究领域:
分析化学
·
电化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）