MATERIALS LETTERS
·
2023
·
IF 3
Zhe Chen,Yu Han,Pengfei Cai,Xiumei Mo,Yunlong Zhang,Jinglei Wu,Binbin Sun
Clinical applications of soft tissue using 3D bio-printing have been limited due to the difficulty of forming bio-ink materials. In this study, we propose a simple and feasible gel suspension printing system with high printing accuracy using a temperature-sensitive material, Pluronic F-127. The detailed printing parameters of the printing system were confirmed. A heart organ model was successfully fabricated using this printing system, and in vitro cell experiments have demonstrated its good biocompatibility. The results showed that the printing system has the practical ability to prepare soft tissues for application.
研究领域:
生物医学工程
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再生医学
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组织工程
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产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2022
·
IF 5.195
Bin Du,Mengmeng Zhao,Yuehua Wang,Lili Yu,Qingqing Jiao,Yimeng Bai,Genyang Cheng
The oxidative stress and activation of the fibrosis pathway are essential pathological mechanisms of acute kidney injury (AKI). In this article, we designed a drug delivery system that could effectively improve oxidative stress and relieve fibrosis by the combination of precise targeting, solubilization, and reducing the toxicity of nano-transport system to strengthen the efficacy of AKI. Folic acid (FA) was used as the targeting molecule, and curcumin (Cur) and resveratrol (Res), which are Chinese medicine monomers with anti-inflammatory and antioxidant effects, were used as model drugs. Here, the targeting nanosystem (Cur/ [email protected] /TPGS) co-loaded with Cur and Res was successfully synthesized. Finally, the comprehensive therapeutic effect of the nanosystem was evaluated through the targeted and pharmacodynamic researches on the AKI models induced by cisplatin (CDDP) in vitro and in vivo . The studies in vitro proved that the nanosystem could not only specifically target HK-2 cells and promote the effective accumulation of Cur and Res in the kidney, but also effectively improve oxidative stress by eliminating reactive oxygen species (ROS), stabilizing mitochondrial membrane potential (MMP), and reducing the expression of apoptosis-related proteins. The studies in vivo showed that the nanosystem could effectively play the role of anti-oxidation, anti-inflammatory and alleviate fibrosis to reduce the apoptosis and necrosis of renal tubular cells. The nanosystem could coordinately repair damaged HK-2 cells by improving oxidative stress, inhibiting inflammation and tissue fibrosis, which provided a new idea for the treatment of AKI.
研究领域:
药学
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纳米技术
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药理学
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肾脏生理学
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治疗学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
·
IF 5.268
Baohuan Wu,Sixiang Sai,Keke Li,Xingchen Sun,Jingtian Han,Baocheng Tian
Drugs that are topically applied on the eyes have low bioavailability, which has always been an important problem. In this study, maleimide functionalized, voriconazole (VCZ) loaded mixed micelles (Mal-VCZ-MM) were designed. Pluronic F127 and phospholipid were used as materials, and maleimide was used as an adhesive. The prepared Mal-VCZ-MM was nearly spherical with a particle size of 84.45 ± 1.39 nm and a zeta potential of − 20.3 ± 0.29 mV. The encapsulation efficiency of Mal-VCZ-MM was 95.33 ± 0.06%, and it had high stability with a critical micelle concentration value of 1.28 × 10 -4 mg/mL. CCK-8 assay showed that its cytotoxicity was lower than that of free VCZ solution (VCZ-Sol). Both quantitative and qualitative analyses of the HCE-T cellular uptake showed that the cellular internalization of Mal-C6-MM was significantly stronger than that of C6-MM. The endocytosis pathway was macropinocytosis-mediated, cavernous-mediated, and energy-dependent. In vitro results against Candida albicans showed that the diameters of the antifungal inhibition zones of VCZ-Sol, VCZ-MM, and Mal-VCZ-MM were 15.5 ± 0.50 mm, 24.0 ± 0.71 mm, and 31.5 ± 1.12 mm, respectively. The antifungal effect of Mal-VCZ-MM was significantly higher than that of VCZ-Sol and VCZ-MM ( P < 0.001). This study shows that Mal-VCZ-MM is a highly effective hydrophobic ophthalmic drug-delivery carrier that can improve the therapeutic effect of the drug.
研究领域:
药剂学
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药物递送
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眼科学
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微生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 54
·49263-49271
·
IF 3.289
Shaoyu Lü,Nannan Gao,Zhen Cao,Chunmei Gao,Xiubin Xu,Xiao Bai,Chen Feng,Mingzhu Liu
Tumor-specific drug delivery is still a challenge in cancer therapy. Passive tumor targeting strategies, such as the enhanced permeability and retention (EPR) effect, cause nanocarriers to accumulate in tumors. However, this strategy can not provide specific tumor targeting. In this study, Pluronic F127 (PF127), a block copolymer which can inhibit drug efflux transporters in cancer therapy, was modified to form tumor-specific micelles with a natural polysaccharide, chondroitin sulfate (ChS), which imparts the site-specific property. A facile and efficient method based on Schiff base reaction was developed to facilitate both basic and clinical research. A series of PF127–ChS micelles with different ratios of PF127 and ChS were fabricated and evaluated in terms of size, morphology, drug loading efficiency and drug release behavior. Spherical micelles with a mean diameter of 155–241 nm were obtained. Their critical micelle concentration (CMC) was significantly reduced in contrast to PF127 micelles and their stability was enhanced. Doxorubicin (DOX) was loaded into the hydrophobic core of PF127 or adsorbed by ChS through electrostatic interactions with the negative charges of chondroitin sulfate. In vitro DOX release studies showed that DOX release from the micelles was enhanced at acidic pH values compared to physiological pH. A cytotoxicity assay (MTT) determined that the micelles possess significantly lower toxicity. Confocal microscopy and flow cytometry analysis indicated that DOX loaded micelles could efficiently release DOX inside cells by specific cellular uptake. These outcomes revealed that PF127–ChS micelles could be exploited as carriers for anti-tumor drugs for site-specific therapy of solid tumors.
研究领域:
药剂学
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癌症治疗
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药物递送系统
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聚合物化学
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生物医学工程
✓ 已验证
DOI
高影响因子
Materials
·
2015
·Vol. 8
·期 2
·379-391
·
IF 2.651
Yanchao Liang,Zhihui Su,Yao Yao,Na Zhang
A novel polymer-drug conjugate was prepared by the chemical reaction between the copolymer Pluronic P123 and the docetaxel via a pH sensitive hydrazone bond. These pluronic P123-docetaxel (DTX) conjugates (P123-DTX) could form the stable drug-loaded materials that can self-assemble into the defined nano-micelles in aqueous solution because of their obvious amphiphilic property and low critical micelle concentration. The spherical morphology and particle size of the prepared nano-micelles were characterized by transmission electron microscopy and dynamic light scattering, respectively. Moreover, after the introduction of pH sensitive hydrazone bond, P123-DTX micelle showed a pH dependent drug release behavior. At pH 5.0 (in 48 h), the cumulative release amount of DTX were ~84.9%, which is about six times higher than that at pH 7.4. The prepared novel p123-DTX conjugates may offer a great benefit for drug delivery and controlling the drug release.Keywords:docetaxel;conjugates;nano-micelles;stability;pH controlled release
研究领域:
药剂学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
Nanomedicine
·
2018
·
IF 5.005
Shuang Xie,Yan C Gong,Xiang Y Xiong,Zi L Li,Yue Y Luo,Yu P Li
Aim: To explore the better efficacy of targeted folic acid (FA)-Pluronic 85-poly(lactide-co-glycolide) (FA–P85–PLGA) polymersome in oral insulin delivery. Materials & methods: The cytotoxicity of the polymers, in vitro qualitative and quantitative cellular uptake and the internalization mechanism of insulin-loaded FA–P85–PLGA and PLGA–P85–PLGA polymersomes were studied with the human colon adenocarcinoma cells (Caco-2 cells). Their pharmacodynamics and pharmacokinetics properties were also studied with diabetic rats. Results & conclusion: Polymersomes have shown good biocompatibility. Polymersomes are mainly localized within the cytoplasm of Caco-2 cells from fluorescence microscopy images. FA–P85–PLGA exhibited higher cellular uptake than PLGA–P85–PLGA polymersomes and free fluorescein isothiocyanate-labeled insulin (FITC–insulin) did. The uptake process of targeted polymersomes included clathrin- and caveolae-mediated endocytosis, macropinocytosis and the folate receptor-mediated endocytosis. Insulin-loaded FA–P85–PLGA showed better hypoglycemic effects than insulin-loaded PLGA–P85–PLGA.
研究领域:
药学
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药物递送系统
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生物医学工程
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2020
·
IF 1.701
Ziling Li,Yousheng Huang,Shengfeng Peng,Xing Chen,Liqiang Zou,Wei Liu,Chengmei Liu
We prepared liposomes consisting of different ratios of phospholipid and pluronic F127 (F127-Lps), then evaluated the effect of matrix on the morphology, stability and carriers’ property of liposomes. The particle size of liposomes was significantly decreased after pluronic F127 modification. Pluronic F127 at low ratio may incorporate into liposomes and form spherical and lamellar structure, while pluronic F127 at high ratio can form mixed aggregates with spherical micelles and lamellar vesicles. The membrane stability of liposomes was enhanced with increasing of pluronic F127 ratio. Curcumin was encapsulated into F127-Lps (cur-F127-Lps) with an entrapment efficiency of 89%. The higher the ratio of pluronic F127 was, the slower the release rate of curcumin. First order model was the main release mechanism for curcumin loaded liposomes. These results are useful for designing liposome-based food delivery systems that can be used in functional foods.
研究领域:
食品科学
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药剂学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Jinming Zhang,Xiaobin Fang,Zeyong Li,Hon Fai Chan,Zhixiu Lin,Yitao Wang,Meiwan Chen
Brusatol (Bru) exhibits promising anticancer effects, with both proliferation inhibition and chemoresistance amelioration activity. However, the poor solubility and insufficient intracellular delivery of Bru greatly restrict its application. Herein, to simultaneously utilize the advantages of Pluronics as drug carriers and tumor microenvironment-responsive drug release profiles, a flexible amphiphilic copolymer with a polymer skeleton, that is, Pluronic® F68 grafting with linoleic acid moieties by redox-reducible disulfide bonds (F68-SS-LA), was synthesized. After characterization by 1H-nuclear magnetic resonance and Fourier transform infrared spectroscopy, the redox-sensitive F68-SS-LA micelles were self-assembled in a much lower critical micelle concentration than that of the unmodified F68 copolymer. Bru was loaded in micelles (Bru/SS-M) with high loading efficiency, narrow size distribution, and excellent storage stability. The redox-sensitive Bru/SS-M exhibited rapid particle dissociation and drug release in response to a redox environment. Based on the enhanced cellular internalization, Bru/SS-M achieved higher cytotoxicity in both Bel-7402 and MCF-7 cells compared with free Bru and nonreducible micelles. The improved anticancer effect was attributed to the remarkably decreased mitochondrial membrane potential and increased reactive oxygen species level as well as apoptotic rate. These results demonstrated that F68-SS-LA micelles possess great potential as an efficient delivery vehicle for Bru to promote its anticancer efficiency via an oxidation pathway.
研究领域:
药剂学
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肿瘤学
·
生物材料
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2016
·
IF 3.994
Xiao-Bin Fang,Jin-Ming Zhang,Xi Xie,Di Liu,Cheng-Wei He,Jian-Bo Wan,Mei-Wan Chen
Curcumin (Cur) is a highly pleiotropic anticancer agent that inhibits cell proliferation and induces apoptosis in cancer cells. A variety of nano-systems constituted by polymer–drug conjugates have been designed to overcome its shortages on water solubility, chemical instability, and poor bioavailability. However, most of them suffer from ineffective release of Cur in cancer cells in vivo . This work developed a novel flexible acid-responsive micelle formulation by covalently conjugating Cur on the hydrophilic terminals of pluronic F68 chains via cis -aconitic anhydride linkers. The synthesized F68-Cis–Cur conjugates can readily precipitate to form homogeneous micelles with average size about 100 nm in aqueous solution. In acid environments, F68-Cis–Cur conjugates would break down and subsequently release Cur rapidly, for the reason of pH-sensitive cleavage of cis -aconitic anhydride linkers. In vitro anticancer activity tests demonstrated that F68-Cis–Cur micelles induced higher cytotoxicity against both A2780 and SMMC 7721 cells than free Cur. It provided a larger decrease of mitochondrion membrane potential and induced cellular apoptosis. F68-Cis–Cur micelles remarkably increased cellular uptake of Cur than free Cur through caveolae-mediated endocytosis in an energy-dependent manner. This study demonstrates F68-Cis–Cur conjugation as a promising tool for improving intracellular drug delivery in cancer therapy.
研究领域:
药剂学
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肿瘤学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2023
·
IF 2.9
Tian Yi Wu,Wen Jie Cao,Zi Ling Li,Yan Chun Gong,Xiang Yuan Xiong
Drugs with different solubility can be selectively embedded into polymersomes with the hydrophilic core and hydrophobic bilayer. Novel folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes were constructed and used for the co-delivery of paclitaxel (PTX) and doxorubicin (DOX) to improve their inhibitory effect over cancer cells. The particle size of blank polymersomes was mainly distributed below 125 nm. The release of PTX and DOX from polymersomes showed an initial burst release followed by a sustained and slow release. The in vitro cytotoxicity data showed that the targeted co-loaded polymersomes (PTX&DOX FA-Ps) exhibited better inhibitory effect than single-loaded polymersomes and free drugs did. Furthermore, PTX&DOX FA-Ps showed the synergistic therapeutic effect over OVCAR-3 cancer cells. The cellular uptake results also showed that folate modified polymersomes had excellent targeting performance. Therefore, the folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes have potential application value as novel drug carriers to co-deliver PTX and DOX.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Fayang Li,Xianhuang Chen,Yuanqiao He,Zhiping Peng
The development of nanocarriers to prolong the residence time and enhance the permeability of chemotherapeutic drugs on bladder mucosa is important in the postsurgery treatment of superficial bladder cancers (BCs). Here, the mucoadhesive HA-SH/PF127 nanogels composed of a temperature-sensitive Pluronic F127 (PF127) core and thiolated hyaluronic acid (HA-SH) shell were prepared by the emulsification/solvent evaporation method. The nanogels were constructed through the thiol-maleimide click reaction in the HA-SH aqueous side of the oil–water interface and self-oxidized cross-linking thiols between HA-SH. The HA-SH/PF127 nanogels prepared at different thiol-to-maleimide group molar ratios, water-to-oil volume ratios, and cross-linking reaction times were characterized regarding hydrodynamic diameter (Dh) and zeta potential (ζ), and the optimal formulation was obtained. The excellent mucoadhesive properties of the HA-SH/PF127 nanogels were evaluated by using the mucin particle method. Doxorubicin (DOX) was encapsulated in the PF127 core of DOX@HA-SH/PF127 nanogels with a high loading efficiency (87.5%) and sustained release from the nanogels in artificial urine. Ex vivo studies on porcine bladder mucosa showed that the DOX@HA-SH/PF127 nanogels enhanced the penetration of the DOX into the bladder mucosa without disrupting the mucus structure or the bladder tissue. A significant dose-dependent cytotoxic effect of DOX@HA-SH/PF127 nanogels on both T24 and MB49 cells was observed. The present study demonstrates that the mucoadhesive HA-SH/PF127 nanogels are a promising intravesical drug delivery system for superficial BC therapy.
研究领域:
药剂学
·
肿瘤学
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2014
·Vol. 25
·期 29
·295103
·
IF 3.672
Jun Chen,Xiao-Quan Yang,Yuan-Zheng Meng,Huan-Huan Huang,Meng-Yao Qin,Dong-Mei Yan,Yuan-Di Zhao,Zhi-Ya Ma
Probe bismuth sulfide modified with Pluronic F127 (Bi2S3-PF127), which has high biocompatibility and dispersibility, is synthesized using triblock copolymer Pluronic F127 to modify hydrophobic Bi2S3 nanoparticles that are prepared by a hot injection method. TEM results show that most of the probe has a length of about 14.85 ± 1.70 nm and a breadth of about 4.79 ± 0.63 nm. After injected into the tail vein of a mouse, the probe has obvious CT contrast enhancement capability from x-ray CT imaging results. Meanwhile, the probe's in vivo toxicity is also studied. It is found that hematoxylin and eosin stains of major organs have no change. A biochemical analysis (alanine aminotransferase and aspartate aminotransferase) prove the probe has no adverse effects. The results of a blood analysis (white blood cell count, red blood cell count, hemoglobin, and platelet count) are also normal. The biological distribution of Bi by ICP-AES shows that most of nanoparticles are cleaned out after injection 48 h, and the circulation half-life of the probe is 5.0 h, suggesting that Bi2S3-PF127 has a long circulation and indicating that the Bi2S3-PF127 probe has good biocompatibility and safety.
研究领域:
医学影像学
·
纳米医学
·
毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）