APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2021
·Vol. 193
·期 4
·1116-1128
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IF 2.926
Wang Qian,Wu Xiaomei,Lu Xinyi,He Yucai,Ma Baodi,Xu Yi
Currently, the biotechnological preparation of fragrances using natural materials attracted growing attention. Enzymatic synthesis of vanillin from isoeugenol by recombinant isoeugenol monooxygenase from Pseudomonas nitroreducens Jin1 was systematically investigated herein. With series of work on the construction of recombinant E. coli over-expressing isoeugenol monooxygenase, optimization of the culture conditions for enzyme production and reaction process for converting isoeugenol into vanillin, an increase of 22-fold in the enzyme activity (2050 U/L) was obtained, and the conversion was significantly increased at high substrate concentration with the aid of magnetic chitosan membrane for product isolation in situ. Under optimal conditions, the product concentration and space-time yield reached 252 mM and 115 g/L/d, respectively, and vanillin was obtained in 82.3% yield and > 99% purity in the gram preparative scale. The developed bioprocess showed application potential for efficient preparation of vanillin from inexpensive natural resources.
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PETROLEUM SCIENCE AND TECHNOLOGY
·
2019
·
IF 1.07
Lin Li,Xuwei Liu,Huijing Wang,Chaocheng Zhao,Qiyou Liu,Xiufang Shang
Environmental adaptability and biodegradability of oil pollutants on functional bacteria are two important keys to bioremediation technologies. Pseudomonas sp. LKY-5 revealed strong biological activities in high salinity (3%) and high alkalinity (pH 9.0). N-hexadecane, phenanthrene, pyrene and carbazole could be directly utilized, and inhibited by the presence of DBT. Sulfur compounds and alkanes in No. 0 diesel and FCC diesel were effectively degraded. The concentrations of short chain alkanes were increased significantly after degradation. It can be seen that strain LKY-5 is an excellent bacterial candidate for bioremediation of oil-contaminated sites and sediments.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Chaofan Zhang,Erya Cai,Xiaoliang Qi,XinXin Ge,Yajing Xiang,Jiajia Wang,Ying Li,Lizhang Lv,Hui Zheng,Yuna Qian,Wei Dong,He Li,Jianliang Shen
Current approaches to treating infected pressure ulcers (IPUs) tend to lack specificity. Such injuries frequently exhibit extended inflammation and slow tissue regeneration, largely due to a surplus of M1 macrophages in the impacted regions. While immunomodulatory hydrogels offer potential for targeted IPU care, facilitating a safe and efficient shift from M1 to M2 macrophages continues to be a considerable challenge. The wound's harsh microenvironment—marked by unchecked infections and reactive oxygen species—often undermines the effectiveness of these hydrogels. We unveil a custom-engineered hydrogel dressing specifically designed to have immunomodulatory properties for expediting the healing of IPUs. Consisting of intertwined polymer networks, this hydrogel incorporates gallium (Ga) ions-triggered self-assembled glycyrrhizic acid (GA). Crucially, both in vitro and in vivo examinations, along with RNA sequencing assessments, demonstrate that the Ga/GA combination successfully reduces the M1/M2 macrophage ratio under inflamed circumstances, thereby contributing to improved IPU wound recovery. In summary, our proposed immunomodulatory strategy provides a simple, secure, and highly efficacious method for managing IPUs.
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Biofilm
·
2024
·
IF 5.9
Shi-Liang Xiang,Kai-Zhong Xu,Lu-Jun Yin,Yong Rao,Bo Wang,Ai-Qun Jia
Pseudomonas aeruginosa is recognized globally as an opportunistic pathogen of considerable concern due to its high virulence and pathogenicity, especially in immunocompromised individuals. While research has identified several endogenous quorum sensing (QS) signaling molecules that enhance the virulence and pathogenicity of P. aeruginosa , investigations on exogenous QS signaling molecules or modulating factors remain limited. This study found that dopamine serves as an exogenous QS signaling molecule or modulating factor of P. aeruginosa PAO1, enhancing the production of virulence factors and biofilms. Compared to the control group, treatment with 40 μM dopamine resulted in a 33.1 % increase in biofilm formation, 68.1 % increase in swimming mobility, 63.1 % increase in swarming mobility, 147.2 % increase in the signaling molecule 3-oxo-C12-HSL, and 50.5 %, 28.5 %, 27.0 %, and 33.2 % increases in the virulence factors alginate, rhamnolipids, protease, and pyocyanin, respectively. This study further explored the mechanism of dopamine regulating the biofilm formation and virulence of P. aeruginosa PAO1 through transcriptome and metabolome. Transcriptomic analysis showed that dopamine promoted the expression of virulence genes psl, alg, lasA, rhlABC , rml , and phz in P. aeruginosa PAO1. Metabolomic analysis revealed changes in the concentrations of tryptophan, pyruvate, ethanolamine, glycine, 3-hydroxybutyric acid, and alizarin. Furthermore, KEGG enrichment analysis of altered genes and metabolites indicated that dopamine enhanced phenylalanine, tyrosine, and tryptophan in P. aeruginosa PAO1. The results of this study will contribute to the development of novel exogenous QS signaling molecules or modulating factors and advance our understanding of the interactions between P. aeruginosa and the host environment.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Xinyi Tao,Kun Zou,Mengze Yuan,Yixuan Wen,Benben Liu,Taiping Qing,Bo Feng,Peng Zhang
Microbial synthesis is an effective method for carbon and nitrogen recovery in the biological nitrogen removal process. The recycling of alginate-like exopolymers has attracted the attention of many researchers; however, the composition of the polymers and alginate differs. In this study, the capacity and mechanism of a mucoid Pseudomonas aeruginosa to remove nitrate and synthesize alginate were investigated. The results showed that the P. aeruginosa achieved higher nitrate removal efficiency (95.3 %) and alginate yield (298 mg/L) after 36 hours of microaerobic cultivation compare to aerobic condition. The optimal conditions for nitrogen removal and alginate recovery are 35 °C, pH 7 and C/N 15. The transcriptional activities of denitrifying genes in P. aeruginosa were upregulated at the low dissolved oxygen level. Although O 2 may upregulate the expression of alginate synthesis key genes ( algD , alg8 and alg44 ) by activating AlgZ-AlgR two-component signal transduction system, microaerobic condition facilitates P. aeruginosa proliferation and results in a higher alginate yield. Moreover, the P. aeruginosa strain was further coupled with an anoxic/aerobic reactor for deeper nitrogen removal, and the nitrate removal efficiency and alginate yield were maintained above 91 % and 100 mg/L, respectively. The conversion efficiency of sodium acetate to alginate reached 15 %. This work provides an economically and environmentally sustainable strategy for wastewater treatment and the recovery of high-value bioresource.
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WATER ENVIRONMENT RESEARCH
·
2024
·Vol. 96
·期 4
·e11022
·
IF 2.5
Yin-liang Lin,Ming-xin Zhao,Ya-lan Yu,Sheng-li Liu,Min Li,A-li Jiang,Min Deng
A microfluidic strategy of smart calcium alginate (CA) capsules is presented to immobilize Pseudomonas aeruginosa to treat oil slicks effectively. The capsule wall is embedded with poly ( N -isopropyl acrylamide) sub-microspheres as thermo-responsive switches. CA capsules, with a diameter of 3.26 mm and a thin wall thickness about 12.8 μm, have satisfying monodispersity, cavity structure, and dense surface structures. The capsules possess excellent encapsulation of bacteria, which are fixed in a restricted space and become more aggregated. It overcomes the disadvantages of a long fermentation production cycle, easy loss of bacteria, and susceptibility to shear effect. The smart CA capsules immobilized with bacteria treat model wastewater containing soybean oil or diesel and display favorable fermentation ability. The capsules can effectively treat oil slicks with high concentration, and it is an economical way for processing oily wastewater. Practitioner Points A thermo-responsive calcium alginate capsule was prepared by microfluidic strategy. Pseudomonas aeruginosa is environmentally friendly in treating oil slicks. The capsules, immobilized bacteria, treat oil slicks effectively. This study provides an economical way for processing different oily water.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Yuyu Zhang,Tao Zhang,Wanling Cai,Gary Owens,Zuliang Chen
While microbial technologies, which are considered to be environmentally friendly, have great potential for the recovery of rare earth elements (REEs) from mining wastewater, their applications have been restricted due to a lack of efficient biosorbents. In this study, a strain of Pseudomonas psychrotolerans isolated from yttrium-enriched mine soil was used to recover yttrium (Y(III)) from rare-earth mining wastewater. At an initial Y(III) dose of 50 mg L −1 , the amount of Y(III) adsorbed by P. psychrotolerans reached 99.9 % after 24 h. Various characterization techniques revealed that P. psychrotolerans adsorbed Y(III) mainly through complexation of oxygen-containing functional groups and electrostatic interactions. A high level of adsorption efficiency (>99.9 %) was maintained after five consecutive adsorption/desorption cycles, indicating that P. psychrotolerans was highly reusable. While the efficiency of adsorbing Y(III) by P. psychrotolerans decreased (34.4 %) in actual rare earth mining wastewater, selectivity toward other REEs (≤ 18.4 %) was still observed. Consequently, this study provides a promising green, environmentally friendly and sustainable microbial approach for the selective recovery of REEs from rare earth wastewater.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-19
·
IF 15.7
Zhang Yu,Zhang Tingting,Xiao Xue,Wang Yejun,Kawalek Adam,Ou Jinzhao,Ren Anmin,Sun Wenhao,de Bakker Vincent,Liu Yujie,Li Yuelong,Yang Liang,Ye Liang,Jia Ning,Veening Jan-Willem,Liu Xue
With the rise of antibiotic-resistant bacteria, non-antibiotic therapies like gallium gain increasing attention. Intravenous gallium nitrate is under Phase II clinical trials to treat chronic Pseudomonas aeruginosa infections in cystic fibrosis patients. However, its clinical efficacy is constrained by the achievable peak concentration in human tissue. To address this limitation, we apply a genome-wide CRISPR interference approach (CRISPRi-seq) to identify potential synergistic targets with gallium. We classify the essential genes by response time and growth reduction, pinpointing the most vulnerable therapeutic targets in this species. In addition, we identify a highly conserved gene, fprB , encoding a ferredoxin-NADP⁺ reductase, whose deletion sensitizes P. aeruginosa to gallium, lowering its MIC by 32-fold and shifting mode of action from bacteriostatic to bactericidal. Further investigation reveals that FprB plays a critical role in modulating oxidative stress induced by gallium, via control of iron homeostasis and reactive oxygen species accumulation. Deleting fprB enhances gallium’s efficacy against biofilm formation and improves outcomes in a murine lung infection model of P. aeruginosa , suggesting FprB is a promising drug target in combination with gallium. Overall, our data show CRISPRi-seq as a powerful tool for systematic genetic analysis of P. aeruginosa , advancing the identification of novel therapeutic targets.
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Journal of Environmental Sciences
·
2025
·
IF 6.3
Caihong Wang,Jiaxin Zhang,Tong Li,Jingwei Wang,Dan Xu,Qiao Ma
Benzalkonium chloride (BAC) is widely employed as a broad-spectrum biocide and has emerged as a significant environmental pollutant. Polymyxin B (PB) serves as the last-line defense for the treatment of Gram-negative pathogens. Previous studies reported that BAC-adapted Pseudomonas aeruginosa increased the tolerance to PB. Herein, we present the novel finding that the combination of BAC and PB exhibited synergistic antibacterial effects against P. aeruginosa . Time-killing assay demonstrated a significant reduction in bacterial cell viability. Scanning electron microscopy, zeta potential analysis, hydrophobicity measurements, and fluorescence probe analyses collectively revealed severe disruption of the cell envelope and membrane potential induced by the combination of BAC and PB. Transcriptomic analysis revealed that the BAC-PB combination notably downregulated the expression of genes involved in lipid A modification and cell envelope production, including phoPQ, pmrAB, bamABCDE, lptABCDEG, lolB, yidC , and murJ . Additionally, the combination group exhibited augmented production of reactive oxygen species and diminished ATP synthesis. The expression of the genes associated with substance metabolism and energy generation was significantly impeded. This study provides significant implications for the interactions of biocides and antibiotics on Gram-negative pathogens, while also addressing antibiotic resistance and developing the external treatment strategy for Pseudomonas -infected wounds and burns.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 103
·期 2
·903-915
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IF 3.34
Wang Hong,Chu Weihua,Ye Chao,Gaeta Bruno,Tao Huimin,Wang Min,Qiu Zheng
Quorum sensing (QS) is a cell-to-cell communication that is used by bacteria to regulate collective behaviors. Quorum sensing controls virulence factor production in many bacterial species and it is regarded as an attractive target to combat bacterial pathogenicity, especially against antibiotic-resistant bacteria. Chlorogenic acid (CA), abundant in fruits, vegetables, and Chinese herbs, processes multiple activities. In this research, we explored its quorum sensing quenching activity. In Pseudomonas aeruginosa , CA significantly inhibited the formation of biofilm, the ability of swarming, and virulence factors including protease and elastase activities and rhamnolipid and pyocyanin production. CA showed similar inhibitory effects in Chromobacterium violaceum on its biofilm formation, swarming motility, chitinolytic activity and violacein production. We examined the expression of QS-related genes in P.aeruginosa and found these genes were all downregulated by CA treatment. Computational modeling revealed that CA can form hydrogen bonds with all three QS receptors. Caenorhabditis elegans and mouse infection models were employed to explore the anti-virulence ability of CA and its effect on pathogenesis process in vivo . CA extended the survival period and reduced the quantity of P. aeruginosa in nematode gut, showing a moderate protective effect on C. elegans . In mice wound model, CA-treated groups showed an accelerating healing rate and the bacteria number in wound area was also decreased by CA treatment. It is suggested by our research that CA has potential to be used as an anti-virulence factor in P. aeruginosa infection.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
·
IF 4.527
Jian-Jun Jiang,Jun-Feng Wang,Ping Yang,Zhi-Min Xu,Tao He,Qiong Gao,Li-Li Wang,Qu-Sheng Li
The application of palygorskite (PAL) for potentially toxic trace elements (Cd 2+ , Ni 2+ , etc.) remediation in polluted soil can substantially reduce the bioavailability and toxicity of these hazard materials. However, the secretion of organic acids and siderophores by microorganisms might result in the re-mobilization of cadmium (Cd) in PAL-bound forms (PAL-Cd). In this study, the interactive effects between Cd stabilized by PAL and mobilized by siderophores from Pseudomonas fluorescens were performed with four flask-shaking experimental treatments, namely, strain with or without an ability of siderophores production respectively associated with or without PAL-Cd. The GC-MS and UHPLC-MS test methods were used to analyze the concentrations of metabolites. Results showed that the Cd mobilized by strain with siderophores production was 22.1% higher than that of strain without the ability of siderophores production ( p < 0.05). The mobilization of Cd in PAL in turn significantly reduced the siderophores production of Pseudomonas fluorescens by 25.1% ( p < 0.05). The numbers of metabolites significantly up-regulated and down-regulated were 9 and 22 in strain groups with PAL-Cd addition compared with the groups without PAL-Cd, respectively. Metabolomics analysis revealed that the mobilized Cd affects the signal transduction pathway and primary metabolic processes, reduces the metabolic capacity of pentose phosphate pathway, glycolysis and tricarboxylic acid cycle pathway. These changes inhibit the ability of strain to biosynthesize amino acids during the mobilization processes, further reducing the capacity of Pseudomonas fluorescens to produce siderophores. This study provides a useful information on how to select soil Cd-stabilizing materials in a targeted manner and how to avoid Cd re-mobilization by siderophores.
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BIORESOURCE TECHNOLOGY
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2016
·
IF 4.917
Haiying Huang,Kejia Wu,Aman Khan,Yiming Jiang,Zhenmin Ling,Pu Liu,Yong Chen,Xuanyu Tao,Xiangkai Li
Combined pollutants with polycyclic aromatic hydrocarbons (PAHs) and heavy metals have been identified as toxic and unmanageable contaminates. In this work, Pseudomonas gessardii strain LZ-E isolated from wastewater discharge site of a petrochemical company degrades naphthalene and reduces Cr(VI) simultaneously. 95% of 10 mg L −1 Cr(VI) was reduced to Cr(III) while 77% of 800 mg L −1 naphthalene was degraded when strain LZ-E was incubated in BH medium for 48 h. Furthermore, naphthalene promotes Cr(VI) reduction in strain LZ-E as catechol and phthalic acid produced in naphthalene degradation are able to reduce Cr(VI) abiotically. An aerated bioreactor system was setup to test strain LZ-E’s remediation ability. Strain LZ-E continuously remediated naphthalene and Cr(VI) at rates of 15 mg L −1 h −1 and 0.20 mg L −1 h −1 of 800 mg L −1 naphthalene and 10 mg L −1 Cr(VI) addition with eight batches in 16 days. In summary, strain LZ-E is a potential applicant for combined pollution remediation.
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