CHEMICAL ENGINEERING SCIENCE
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2025
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IF 4.3
Ning Bi,Lixiao Sun,Meihua Hu,Wei Song,Jun Xu,Lei Jia
A novel colorimetric/surface-enhanced Raman spectroscopy (SERS) dual-signal nanozyme sensor was developed for the rapid and sensitive detection of Hg 2+ based on peroxidase-like gold-platinum core–shell nanoflowers (Au@Pt NFs). Au@Pt NFs catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue oxidation product, 3,3′,5,5′-tetramethylbenzidinediamine (oxTMB), which exhibits high Raman activity. However, the presence of Hg 2+ inhibited the catalytic activity of Au@Pt NFs, leading to a gradual disappearance of the blue color of oxTMB. Consequently, both the absorbance at 650 nm and the Raman intensity at 1639 cm −1 decrease with increasing Hg 2+ concentration. The limits of detection (LODs) were 3.2 nM for colorimetric and 0.22 nM for SERS. The preparation method of Au@Pt NFs dual-signal nanozyme sensor was simple and allowed for naked-eye colorimetric detection as well as simultaneous SERS signal measurement. In addition, this nanozyme sensor demonstrates excellent selectivity and sensitivity, making it a promising probe for water quality monitoring.
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Shuyan Ruan,Ru Liu,Xi Chen,Qian Huang,Chongxin Xiao,Ruiyun You,Luqiang Huang,Yunzhen Liu,Jingbo Chen,Xiufeng Xiao,Qingqiang Lin,Yudong Lu
As a reactive oxygen species (ROS), excessive production of H 2 O 2 contributes to the development of several diseases such as, inflammation, cancer, and respiratory diseases. Supplementation with endogenous or exogenous antioxidants can scavenge ROS and reduce the oxidation of cellular molecules, thus alleviating the generation of diseases. Therefore, the determination of H 2 O 2 content and its antioxidant activity is of great importance in disease diagnosis and treatment. In this paper, a ratiometric SERS sensor with a flexible cellulose membrane was designed for quantitative detection of H 2 O 2 and assessment of antioxidant activity. First, gold seeds were reduced on bacterial cellulose membrane (BCM) and Au NPs were smoothly deposited on the bacterial cellulose membrane (BCM) using halides to reduce the reduction potential in the growth solution to form a flexible BCM@Au NPs SERS substrate. Afterwards, the oxidation of H 2 O 2 was used to convert 3-mercaptophenylboronic acid (3-MPBA) to the corresponding phenol form 3-hydroxyphenylethanol (3-HTP). The change of substance resulted in a good linear relationship between the intensity ratio corresponding to the two Raman shifts of 881 cm −1 and 995 cm −1 and the H 2 O 2 concentration with a detection limit of 0.0186 μM. This opens up a new method for the detection of H 2 O 2 with high sensitivity and accuracy. In addition, this SERS platform was successfully used for the determination of antioxidant activity. It is promising to be applied to disease diagnosis and efficacy evaluation.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 13
·2016
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IF 5.3
Yanyan Lu,Xuzhou Yuan,Cuiping Jia,Biao Lei,Hongwen Zhang,Zhipeng Zhao,Shuyi Zhu,Qian Zhao,Weiping Cai
Volatile organic compounds (VOCs), particularly monoaromatic hydrocarbon compounds (MACHs), pose a potential risk to the atmospheric environment and human health. Therefore, the progressive development of efficient detection methodologies is a pertinent need, which is still a challenge at present. In this study, we present a rapid and sensitive method to detect trace amounts of MACHs using a bifunctional SERS composite substrate. We prepared an Au/SiO2enhanced layer and a porous Cu(OH)2adsorption layer via microfluidic-assisted gas-liquid interface self-assembly. The composite substrate effectively monitored changes in benzaldehyde using time-varying SERS spectra, and track-specifically identified various VOCs such as benzene, xylene, styrene, and nitrobenzene. In general, the substrate exhibited a rapid response time of 20 s to gaseous benzaldehyde, with a minimum detection concentration of less than 500 ppt. Further experimental assessments revealed an optimum Cu(OH)2thickness of the surrounding adsorption layer of 150 nm, which can achieve an efficient SERS response to MACHs. Furthermore, the recoverable and reusable property of the composite substrate highlights its practicality. This study presents a straightforward and efficient approach for detecting trace gaseous VOCs using SERS, with significant implications in the designing of SERS substrates for detecting other VOCs.Keywords:VOCs;bifunctional copper hydroxide/gold ordered nanoarray composites;self-assembly;fast and sensitive SERS detection;recyclable SERS substrate
研究领域:
环境科学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
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2025
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IF 2.6
Jinran Chen,Xiurui Ke,Zhou Zhou,Wenqi Ye,Hong Liu,Wei Zhang,Xiaohong Liu
We report the fabrication of a substrate with cavity-nanorods and decorated with Ag-nanoplates (C-NR@Ag). The cavities on the substrate are formed by metal assistant chemical etching, and the Ag-nanoplates in the cavities by galvanic cell deposition enhance the SERS performance effectively. Analytes in solution are adsorbed on Ag-nanoplates and located in hot spots, which enhance the SERS performance effectively. The enhancement factor of the Ag-nanoplates decorated on nanorod cavities is calculated to be 3.6 × 106, which is about 3 fold higher than that on the nanorods. The C-NR@Ag substrate is able to detect polychlorinated biphenyls (PCBs) with the lower limit of detection at 1.0 × 10−12 M. Additionally, due to the semi-volatile nature of PCB-77, the lower limit of detection of the C-NR@Ag substrate for PCB-77 was 1.0 × 10−11 M by the non-contact collection method. These results present a novel approach towards enhancing SERS performance and facilitating the rapid detection of PCB-77.
研究领域:
材料科学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Engineering Journal Advances
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2022
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IF 0
Wei Xue,Bo Han,Haojie Ma,Xueyan Hou,Ji-Jiang Wang,Ran Li,Yuqi Zhang
Novel TiO 2 nanotube arrays (TNAs) involved heterojunctions are widely used as photocatalyst for pollutants, while the residues are usually detected by other method. A device which integrates photocatalytic degradation and enhancing detection signals could increase the atom utilization and decrease detection cost. In this work, a novel TNAs/g-C 3 N 4 /AuNPs heterojunction was prepared and used as photocatalyst and SERS substrate simultaneously. Dye of crystal violet was successfully degraded, and the concentration of residue was detected by Raman spectroscopy. There was a good linear correlation between the Raman intensity and the concentration of crystal violet from 10 −10 M to 10 −6 M, and the limit of detection (LoD) was 8.79 × 10 −11 M. Moreover, the prepared heterojunctions were used on wastewater and biological staining detection. In the applications of photocatalytic degradation and detection, the time-dependent SERS intensities demonstrated the photocatalytic degradation achieved more than 95% in 20 min, and almost completed in 25 min, and the heterojunctions showed good recyclability in at least 10 cycles. For SERS property, the original enhancement factor (EF) was calculated to be 7.43 × 10 6 , then the EF descended slightly and remained with less than 90% attenuation after 10 cycles.
研究领域:
环境科学
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材料科学
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分析化学
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DOI
JOURNAL OF MATERIALS SCIENCE
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2021
·Vol. 56
·期 16
·9702-9711
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IF 4.22
Yang Yongmei,Gao Xinna,Yang Sijun,Shen Yuhua,Xie Anjian
Abstract Fabricate surface-enhanced Raman scattering (SERS) substrate with low cost and high performance through simple method is a challenge and worthy of work. Herein, an excellent Cu 2 O SERS substrate with porous octahedron structure was synthesized by brief calcining Cu-MOFs in Ar, air and H 2 /Ar gas environment at 350 °C successively. The as-obtained octahedral Cu 2 O was utilized as SERS substrate to measure dye molecules and organic pollutants, which exhibited splendid sensitivity for the detection of methylene blue and 4-mercaptopyridine (4-MPY) with the ultralow limit of 5 × 10 –9 M and 5 × 10 –10 M, respectively. The maximum relative standard deviation for detection of 4-MPY is as low as 12.3%, which is less than limiting step in quantitative SERS (15.0%). The above excellent results are due to outstanding synergistic effect of electromagnetic enhancement of oxygen vacancy on the Cu 2 O surface and chemical enhancement of the Cu–S bonds formation. The fabricated Cu 2 O possesses superior sensitivity, selectivity and reproducibility for SERS detection of probe molecules with sulfhydryl groups and may be a prospect candidate in the practical application. Graphical Abstract
An excellent Cu 2 O SERS substrate with porous octahedron structure was synthesized by simple calcining Cu-MOFs in Ar, air and H 2 /Ar gas environment at 350 °C successively. The as-prepared Cu 2 O play significant roles in the enhancement of SERS signals for rich-thiol detection due to outstanding synergistic effect of electromagnetic enhancement of oxygen vacancy on the Cu 2 O surface and chemical enhancement of the Cu-S bonds formation. The detection limit of 4-Mercaptopyridine (4-MPY) can be as low as 5×10 –10 M. The porous octahedral Cu 2 O will have potential applications in SERS detection as the substrate.
研究领域:
材料科学
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分析化学
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产品关联
DOI
高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 8
·766
·
IF 5.4
Xiaoxiao Zhou,Shouhui Chen,Yi Pan,Yuanfeng Wang,Naifeng Xu,Yanwen Xue,Xinlin Wei,Ying Lu
In order to improve the detection performance of surface-enhanced Raman scattering (SERS), a low-cost Au@Ag nanorods (Au@Ag NRs) substrate with a good SERS enhancement effect was developed and applied to the detection of malachite green (MG) in aquaculture water and crayfish. By comparing the SERS signal enhancement effect of five kinds of Au@Ag NRs substrates with different silver layer thickness on 4-mercaptobenzoic acid (4-MBA) solution, it was found that the substrate prepared with 100 µL AgNO3had the smallest aspect ratio (3.27) and the thickest Ag layer (4.1 nm). However, it showed a good signal enhancement effect, and achieved a detection of 4-MBA as low as 1 × 10−11M, which was 8.7 times higher than that of the AuNRs substrate. In addition, the Au@Ag NRs substrate developed in this study was used for SRES detection of MG in crayfish; its detection limit was 1.58 × 10−9M. The developed Au@Ag NRs sensor had the advantages of stable SERS signal, uniform size and low cost, which provided a new tool for SERS signal enhancement and highly sensitive SERS detection method development.Keywords:Au@Ag nanorods substrate;surface-enhanced Raman scattering (SERS);malachite green
研究领域:
分析化学
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食品安全检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Ji Yujuan,Ji Yi,Wu Yupeng,Wang Jiaqi,Xue Yu,Huang Haiqin,Yu Yanyan,Sun Dan
Foam cell formation caused by cholesterol accumulation in macrophages is an early warning of atherosclerosis. Therefore, in situ and accurate determination of intracellular and extracellular cholesterol in macrophages is of great significance for the early diagnosis of atherosclerosis and the evaluation of its therapeutic effect. Herein, a surface-enhanced Raman spectroscopy (SERS) and colorimetric dual-response plasmonic nano-sensing system was established based on a double-enzyme cascade catalytic reaction to accurately monitor the changes in cholesterol levels during the occurrence and development of atherosclerosis. Cholesterol oxidase modified on gold nanoparticles can catalyze the oxidation of cholesterol to hydrogen peroxide, which oxidizes 3,3,5,5-tetramethylbenzidine (TMB) to oxidation state TMB (oxTMB) under the catalysis of horseradish peroxidase (HRP). The oxTMB showed a very strong SERS signal under the effect of plasmonic enhancement of gold nanoparticles, and the solution of oxTMB is bright blue compared to colorless TMB. Therefore, the dual-response sensing of cholesterol was realized by monitoring SERS and UV absorption signals of the final product oxTMB. Our results suggest that the free cholesterol in macrophages increases after the treatment of avasimibe, which ultimately leads to apoptosis of macrophages. Another drug, high-density lipoprotein, promoted the efflux of intracellular cholesterol and inhibited the foaming of macrophages, thus delaying the development of atherosclerosis. The colorimetric and SERS double-response plasmonic nanosensor realized highly sensitive, specific, and accurate monitoring of intracellular and extracellular cholesterol levels during drug treatment of atherosclerosis. It is expected to provide an effective tool and methodological reference for the prevention and diagnosis of atherosclerosis and a series of cardiovascular diseases related to cholesterol.
研究领域:
生物医学
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分析化学
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心血管疾病研究
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Huanhuan Li,Wei Sheng,Md. Mehedi Hassan,Wenhui Geng,Quansheng Chen
The abuse of antibiotics has caused gradually increases drug-resistant bacterial strains that pose health risks. Herein, a sensitive SERS sensor coupled multivariate calibration was proposed for quantification of antibiotics in milk. Initially, octahedral gold-silver nanocages (Au@Ag MCs) were synthesized by Cu 2 O template etching method as SERS substrates, which enhanced the plasmonic effect through sharp edges and hollow nanostructures. Afterwards, five chemometric algorithms, like partial least square (PLS), uninformative variable elimination-PLS (UVE-PLS), competitive adaptive reweighted sampling-PLS (CARS-PLS), random frog-PLS (RF-PLS), and convolutional neural network (CNN) were applied for TTC and CAP . RF-PLS performed optimally for TTC and CAP (Rc = 0.9686, Rp = 0.9648, RPD = 3.79 for TTC and Rc = 0.9893, Rp = 0.9878, RPD = 5.88 for CAP). Furthermore, the detection limit of 0.0001 µg/mL for both TTC and CAP was obtained. Finally, satisfactory ( p > 0.05) results were obtained with the standard HPLC method. Therefore, SERS combined RF-PLS could be applied for fast, nondestructive sensing of TTC and CAP in milk.
研究领域:
分析化学
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食品科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Baihui Wang,Huan Jiang,Ruyi Tang,Yaoyu Tan,Xuemin Xia,Xia Zhang
The activity of nanozymes is dependent on their surface for catalysis, and bioconjugation or adsorption of DNA aptamers will promote their activity. This paper aims to develop a novel aptamer sensor, for rapid, sensitive and specific detection of histamine. The design of the aptamer biosensor is based on the adsorption of histamine aptamers onto the surface of Au NPs by bioconjugation, which promotes the peroxidase activity of the nanozymes. Since histamine is strongly selective and specific for histamine aptamers, the histamine aptamer can be detached from the surface of Au NPs and bound to histamine, during which the catalytic activity of the nanozymes falls back due to the detachment of the histamine aptamer, which can be quantitative analysis by the TMB-H 2 O 2 system. The decrease in enzyme catalytic activity became larger with increasing histamine concentration, with a good linear correlation. Finally, the histamine aptamer biosensor was constructed, which has the advantages of ultrasensitive, high-accuracy, and wide linear range. The linear range and detection limit are 10 −11 ∼10 −3 M and 1.22 × 10 −12 M, respectively. With reference to this aptamer sensor design, this approach can also be extended to other schemes where the aptamer is combined with Au NPs as a way to detect the corresponding molecules.
研究领域:
生物化学
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分析化学
·
纳米技术
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产品关联
DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 43
·31809-31819
·
IF 3.9
Long Jia,Yongxia Liu,Yanqing Zhang,Shuofeng Zhang,Xiaowei Cao,Yemin Xu,Yi Zhao,Bin Deng
To facilitate rapid, efficient, and accurate detection of miR-96-5p associated with gastric cancer (GC), we developed a bioanalytical platform by integrating surface-enhanced Raman spectroscopy with lateral flow assay (SERS-LFA). With these SERS-LFA strips, miR-96-5p within the specimen competed with Au rhombic dodecahedron (AuRD) conjugated single-stranded DNA (ssDNA) to bond to the immobilized hairpin DNA (hpDNA) probe on the T line. Consequently, higher abundance of miR-96-5p led to reduced conjugation of AuRD on the T line, thereby resulting in diminished SERS intensity. The biosensor exhibited a detection time of approximately 30 min and demonstrated a low limit of detection (LOD) for miR-96-5p in PBS buffer solution, down to 3.7 fM. To validate its clinical utility for the early diagnosis of patients with different degrees of gastric lesions, we performed quantitative evaluations in cohorts that included healthy individuals, patients with mild intraepithelial neoplasia, patients with severe intraepithelial neoplasia, as well as patients diagnosed with GC. The results obtained from the SERS-LFA strips were in agreement with those obtained from the quantitative real-time polymerase chain reaction (qRT-PCR). Given the accomplishments, this biosensor has significant potential for the clinical diagnosis of GC, offering a promising avenue for timely detection and improved patient prognoses.
研究领域:
生物医学
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分子诊断
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癌症研究
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 8
·2346-2356
·
IF 3.498
Agyekum Akwasi Akomeah,Kutsanedzie Felix Y. H.,Mintah Benjamin Kumah,Annavaram Viswadevarayalu,Braimah Alberta Osei
Norfloxacin is an antibiotic in the fluoroquinolone family licenced for use in animals. However, residues in animal products can have negative consequences for consumers. As a result, residue detection in various food matrices must be considered. Norfloxacin accumulates in animal-derived foods, causing deleterious consequences in humans such as foetal deformity, renal failure and drug resistance. A built-in SERS-Au@Ag nanosensor coupled with GA-PLS was used to rapidly detect norfloxacin in the specimen of the spiked fish muscles due to the threat to human lives. A detection limit of 2.36 × 10 −5 μg/mL was realized in the spiked fish muscle sample for norfloxacin compared to the European Commission’s maximum threshold level of 100 μg/kg, indicating the sensor’s ability to detect and quantify norfloxacin at a relatively lower level. The recovery rates (RC) and coefficient of variation (CV) measured in the spiked fish muscle samples for norfloxacin analytes and their standard solutions were between 99.70–105.00% and 0.17–5.21%, respectively. The low CV values imply the reproducibility of the obtained data. The constructed model recorded residual predictive deviations (RPD) greater than three (3), demonstrating the robustness and resilience of the developed genetic algorithm-partial least squares (GA-PLS) model. GA-PLS-built models predicted all results within 4.07 s, which indicates the nanosensor’s ability to rapidly detect norfloxacin in fish to guarantee safety and public health. The SERS probe holds promise for rapid quantification of norfloxacin at microgram per milliliter level in fish to guarantee safety in commerce. Graphical abstract
研究领域:
食品安全
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分析化学
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纳米技术
·
药理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）