FOOD CHEMISTRY
·
2023
·
IF 8.8
Tongtong Jia,Shaolun Ouyang,Wenrui Chen,Ting Zhang,Mengqi Lu,Xiuying Zhou,Hongtao Lei,Xiaoqun Wei
To get rid of the singularity of the detection of veterinary drug residues in liquid milk, a high-throughput determination was developed for 126 VDRs in 17 categories in liquid milk based on the one-step extraction coupled with LC-HRMS (UPLC-Q-Exactive-Orbitrap-MS). The results showed that adding 2.5 mL of 0.1 mol/L Na2EDTA-McIlvaine in acetonitrile-methanol extractant (50:50, V/V) could effectively improve the response intensity of veterinary drug residues in liquid milk, reduce the matrix effect and eliminate the need for expensive SPE purification. The established external standard quantitative method has a LOD of 0.01–0.30 μg/L for all categories of veterinary drug residues, good linearity in the concentration range of LOQ of 0.03–1.00 μg/L, and correlation coefficients (r 2 ) were all greater than 0.995. The spike recoveries at all three levels were between 80.30% and 119.95%. The positive detection rate of this method in 128 actual samples was 8.59%.
研究领域:
分析化学
·
食品科学
·
兽医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 75
·71596-71611
·
IF 3.289
Chukwunonso Peter Okoli,Gregory Olufemi Adewuyi,Qian Zhang,Qingjun Guo
A solid-phase extraction (SPE), using starch based biopolymer adsorbent, coupled with high performance liquid chromatography–fluorescence/UV detectors (HPLC-FLD/UV) method was developed for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in environmental water samples. Quantitative structure activity relationship was employed for assessing the suitability of epichlorohydrin, 1,6-hexamethylene diisocyanate and 4,4-methylene diphenyl diisocyanate (MDI) based starch, β- and γ-cyclodextrin biopolymers as the SPE sorbent phases, via density functional theoretical (DFT) modeling studies. The DFT parameters showed that MDI based biopolymers exhibited the highest retention potential as the SPE sorbent. The modelled materials were synthesized, characterized and evaluated for the extraction of (PAHs). The results of the screening SPE studies were in agreement with DFT quantum predictions. MDI-starch biopolymer was therefore selected as the best SPE phase. The calibration curves of extracted PAHs were linear in the range of 0.5–50.0 μg L−1, with r2 values of >0.99. The method attained good precisions values of 6.9–26.0 and 0.4–16.6% for UV and FLD detectors respectively; and the method detection limits values of 22.9–155.3 and 0.5–24.2 ng L−1 for UV and FLD detectors respectively. The optimized method was successfully applied for the determination of 16 PAHs in real environmental water samples, and mean recoveries were predominantly >80% for tap and river water samples in both UV and FLD analysis. The values of SPE performance indicators showed that the developed biopolymer phase is better than similar studies reported in literature, and equally comparable to commercially available C18 SPE phases.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Mian Zu,Jinan Jiang,Hui Zhao,Shili Zhang,Yan Yan,Shiwei Qiu,Shuolong Yuan,Jiawei Han,Yue Zhang,Weiwei Guo,Shiming Yang
Irreversible hearing loss induced by aminoglycoside in human through local or systemic administration route negatively impacts quality of life. The aim of this work was to develop and validate an analytical method suitable for the detection and quantification of neomycin in cochlear perilymph of guinea pig after local application. The SupelMIP SPE column was used for the pre-treatment of matrix. Chromatographic separation was conducted by a reversed phase ODS column (100 × 2.1 mm, 3 μm) at 40 °C in gradient mode with 0.2‰ (v/v) HFBA in water and 0.2‰ (v/v) HFBA in acetonitrile as mobile phase , at a flow rate of 0.30 mL/min, with retention time of 3.50 and 3.62 min for internal standard tobramycin and analyte neomycin , respectively. The MS was performed with positive ionization mode, with data acquisition in Multi Reaction Monitor (MRM) mode. This method was proved to be specific, accurate (97.1–115% of nominal values) and precise (CV% < 15%). Calibration curves for matrix matched standard of neomycin ranged from 1.25 to 200 μg/mL, with LOD and LLOQ of 0.625 and 1.25 μg/mL in blank matrix. The matrix effect was corrected to (−0.1) - 1.33 by adding internal standard. The relative SPE recovery values were ≥98.9% in low, medium and high QC samples. Neomycin in matrix proved to be stable under room temperature - and −20 °C, or under three freeze-thawing cycles, or under processing as well. Finally, the proposed method was successfully applied to a toxicokinetics study of neomycin in perilymph after round window membrane (RWM) administration, which was in accordance with threshold shift of auditory brainstem response (ABR) test related to hearing loss.
研究领域:
药理学
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分析化学
·
耳科学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2012
·Vol. 36
·期 5
·980-985
·
IF 2.733
Ziru Lian,Jiangtao Wang
A highly selective sample cleanup procedure combined with molecularly imprinted SPE was developed for the isolation of crystal violet from seawater and seafood samples. The molecularly imprinted polymer was prepared using crystal violet as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. The crystal violet-imprinted polymer was used as the selective sorbent for the SPE of crystal violet. An off-line molecularly imprinted SPE method followed by HPLC with diode-array detection for the analysis of crystal violet was also established. Good linearity on the molecularly imprinted SPE columns was obtained from 0 to 200 μg/L ( R 2 > 0.99). The result demonstrated that the proposed method can be used for the direct determination of crystal violet in seawater and seafood samples. Finally, five samples were analyzed and the following crystal violet concentrations were obtained: 0.92 and 0.52 μg/L in two seawater samples, as well as 0.36 and 0.27 μg/kg in two seafood samples. There is no crystal violet detected in the third seawater sample.
研究领域:
分析化学
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环境科学
·
食品科学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 414
·期 4
·1553-1561
·
IF 4.157
Zhang Wenjuan,Hu Haoyun,Ruan Guihua,Huang Yipeng,Du Fuyou,Chen Zhengyi
In this study, reduced graphene oxide (rGO) hybridized high internal phase emulsions were developed and polymerized as porous carriers for aptamer (5’/5AmMC6/-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3’) modification to enrich human α-thrombin from serum. The structure and properties of the materials were confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscope (FT-IR), and X-ray photoelectron spectra (XPS). The adsorption ability and selectivity were studied and the thrombin was detected with liquid chromatography-mass spectrometry (LC–MS). The adsorption of thrombin onto the sorbent was achieved within 30 min and the desorption was realized using 5.0 mL of acetonitrile/water (80/20, v/v). The thrombin was quantified by LC–MS according to its characteristic peptide sequence of ELLESYIDGR. Graphical abstract
研究领域:
分析化学
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生物材料科学
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生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 167
·期 7
·070504
·
IF 3.12
Muhammad Zaheer,Hongli Xu,Bingbing Wang,Lianwei Li,Yonghong Deng
High ionic conductivity is a prerequisite for the application of solid-state polymer electrolyte towards the safe and high energy density electrochemical devices. Here we report the preparation and properties of an in-situ polymerized comb-like copolymer-based SPE (PLA/PEG-SPE) with high ionic conductivity from methyl acrylate functionalized poly(D,L-Lactide) (PLAMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA). A remarkably high ionic conductivity value of 6.9 × 10−5 S cm−1 at ambient temperature and a maximum ionic conductivity of 4.3 × 10−4 S cm−1 at 60°C were detected, with an activation energy of 0.2 eV and a Li+ transference number (tLi+) of 0.36. The PLA/PEG-SPE exhibits a wide electrochemical stability window up to 4.6 vs. Li/Li+ and very good lithium metal electrode compatibility. Solid-state LiFePO4/SPE/Li cells with integrated cathode and lithium metal deliver superior cycling stability with high discharge capacities (149 mAh g−1 as the initial specific capacity) and high capacity retention (exceeded 82% of its initial specific capacity) at 0.2 C at 60°C. The solid-state cells are also capable of being cycled at room temperature at 0.2 C. This work highlights a facile, in-situ fabrication strategy involving a vinyl-functionalized PLA precursor that yields a high-performance ion-conducting membrane attractive for lithium metal battery applications.
研究领域:
材料科学
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电化学
·
能源存储
·
聚合物化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Guihua Gao,Yanyan Xing,Tingting Liu,Jian Wang,Xiaohong Hou
Four water stable metal-organic frameworks (MOFs), MIL-101(Cr), MIL-100(Fe), MIL-53(Al), and UiO-66(Zr), were used as adsorbents for concentration of four androgens and progestogens from water. The adsorption abilities of target analytes on the four MOFs were investigated by experimental and molecular simulation strategies, and UiO-66(Zr) was ultimately selected as the sorbent to develop a porous membrane protected micro-solid-phase extraction (μ-SPE) method for simultaneous extraction androgens and progestogens in environmental water samples followed by LC-MS/MS detection. Several variables affecting the extraction efficiency were systematically investigated, including pH, salt concentration, extraction time, kind of desorption solvent, and desorption time. Under the optimized conditions, excellent linearity was obtained in the range of 20.32–2032 ng/L for T, 10.24–1024 ng/L for P, 7.624–762.4 ng/L for TP , and 8.376–837.6 ng/L for MPA . Limits of detection (LODs) and limits of quantification (LOQs) were 10 ng/L and 20 ng/L for T, 4.0 ng/L and 10 ng/L for P, 2.0 ng/L and 7.0 ng/L for TP, and 3.0 ng/L and 8.0 ng/L for MPA, respectively. The recoveries were ranged from 80.5% to 102.4%. Furthermore, the μ-SPE device could be used for at least 10 extractions (RSD < 10.0%). Meanwhile, comparison was made with solid-phase extraction, and the developed method was demonstrated to be a convenient and eco-friendly pretreatment method for water samples.
研究领域:
环境科学
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分析化学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Mengmeng Song,Xianjie Sheng,Genyi Ye,Zhuicheng Xu,Xinru Li,Tong Xie,Haishan Deng,An Kang
Gut microbiota-derived tryptophan (TRP) metabolites, especially the indole derivatives, function as critical modulators of intestinal immune function and integrity via modulating the aryl hydrocarbon receptor pathway. Selective enrichment of these indole metabolites in biological samples is important for quantitative determination by routine methods such as HPLC. Here, we report a molecularly imprinted polymer (MIP) for solid-phase extraction (SPE) of TRP-derived indole metabolites from faeces of mice. The MIP was synthesized by surface polymerization by using indole-3-acetic acid (IAA) and acrylamide after cross-linking by ethylene glycol dimethacrylate (EGDMA) and initialed by 2′,2-azobisisobutyronitrile (AIBN). The MIPs were then characterized by transmission electron microscope (TEM) and fourier transform infrared spectroscopy (FTIR), and the adsorption capacity, selectivity and reusability of MIPs were systematically evaluated. Results showed that IAA-MIPs showed a uniformly distributed nanoshell layer with a thin shell thickness of 26 nm . The IAA-MIPs could selectively adsorb IAA, indole-3-propionic acid (IPA), and indole-3-lactic acid (ILA) in a mixed solution that also contains TRP and tyrosine. The adsorption capacity of IAA-MIPs only slightly decreased with the increase of recycling use. As purification material for SPE, the IAA-imprinted polymers (IAA-MIPs) were successfully applied to extract IAA, IPA, and ILA from normal and colitis mice for HPLC determination. Collectively, these surface molecularly imprinted polymers could find extensive use to selective enrichment of microbiota-derived indole metabolites in biological samples.
研究领域:
微生物学
·
分析化学
·
胃肠病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yan Zhang,Yaqian Zhou,Ping Liu,Yufeng Zhang,Yiqun Wan,Fan Liu,Genlin Sun,Lihua Chen,Jun Wang
A rapid and efficient method is highly desired for the determination of phenylurea herbicides (PUHs) in edible and medicinal herbs. Herein, we report a dispersive solid-phase extraction (d-SPE) method using a metal-organic framework Zn 4 O(NTB) 2 as the adsorbent. The microporous Zn 4 O(NTB) 2 can selectively extract targets while excluding matrix substances. Under optimized conditions, this method exhibits high sensitivity, with a limit of detections (LODs) and limits of quantification (LOQs) in the range of 0.09–0.32 ng mL -1 and 0.32–1.08 ng mL -1 , respectively. Moreover, the recoveries reach the range of 69.8 %-83.3 % with relative standard deviations below 6.2 %. The optimized adsorption efficiency of PUHs can be rapidly achieved in a short time of 10 min. Additionally, the adsorbent can be facilely regenerated with methanol washing. Furthermore, computational simulations reveal the guest-host interactions involving hydrogen bonding, π-π stacking interactions, and Van der Waals interactions. Practically, the established method has been successfully validated for extracting PUHs from Dendrobium candidum and Ganoderma lucidum samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Shuang Chen,Yue Qiao,Youwei Jiang,Wei Qiu,Shuang Zang,Jing Zhang,Xianshi Wang,Jun Ma
Halogenated aromatic disinfection byproducts (DBPs) are gradually receiving attention due to their high detection frequency and usually higher toxicity than regulated DBPs. In this study, we established a solid phase extraction (SPE)-LC-MS/MS method to simultaneously trace analyze 59 halogenated aromatic DBPs. The limits of detection and limits of quantification of halogenated aromatic DBPs ranged from 0.03 ng/L to 135.23 ng/L and 0.1 ng/L to 450.76 ng/L, respectively. The range of recoveries and relative standard deviation (RSD) in river water were between 72.41 % to 119.54 % and 1.86 % to 16.03 %, respectively. Therefore, this method can be used to accurately analyze trace levels of halogenated aromatic DBPs in drinking water. The occurrence and transformation of halogenated aromatic DBPs were explored based on this method. In the chlorinated simulated source water and chlorinated river water, 20 and 45 halogenated aromatic DBPs were determined, respectively. The active halogen species (HOCl, HOBr, and HOI) first reacted with natural organic matter (NOM) to form halogenated aromatic DBPs. Then, chlorine further reacted with the halogenated aromatic DBPs to convert them into small-molecule halogenated aliphatic DBPs through oxidation, electrophilic substitution, and hydrolysis reaction, etc. In the chlorinated simulated source water, chlorinated river water, and tap water, the toxicity contribution of bromoacetic acids (Br-HAAs) accounted for the majority (> 71.16 %). Given that halogenated aromatic DBPs are intermediate products of halogenated aliphatic DBPs, controlling the formation of halogenated aromatic DBPs is beneficial in decreasing the formation of halogenated aliphatic DBPs, thereby diminishing the toxicity of drinking water.
研究领域:
环境科学
·
分析化学
·
公共卫生
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2019
·Vol. 83
·期 2
·173-181
·
IF 1.552
Li Mingxue,Bai Ligai,Sun Fanrong,Peng Shan,Liu Haiyan,Ma Zhengyue
Abstract A four-hydrogen furfuryl-based polymer monolithic column for solid-phase extraction was prepared via in situ radical polymerization, using tetrahydrofurfuryl methacrylate as the monomer. The monolith was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy and nitrogen adsorption–desorption method, showing relatively uniform porous structure with micro-, meso- and macro-pores and a relatively high surface area of 54.99 m 2 g −1 . Online solid-phase extraction–high-performance liquid chromatography was carried out to quantitatively analyze six alkaloids, including caffeine, hydroxy-α-sanshool, hydroxy-β-sanshool, hydroxy-γ-sanshool, hydroxy-γ-isosanshool and γ-sanshool in rat plasma. The results show that the prepared polymer monolithic sorbent exhibits good clean-up ability for bio-matrix, as well as good extractive ability for the six concerning alkaloids in rat plasma. Method validation shows that the correlation coefficients of the linear regressions are higher than 0.998 and the accuracy represented by spiked recovery ranges in 94.37–100.7%, 99.52–101.1%, 98.93–100.8%, 98.36–101.6%, 99.22–101.2% and 99.86–101.4% for the six alkaloids, respectively, as well as the LOD ranges in 2.10–3.90 ng mL −1 and the LOQ in 6.35–10.14 ng mL −1 . Compared to the reference works, the present work is a simple and effective method for online extraction and simultaneous determination of six alkaloids in plasma with high accuracy and low cost. Graphic Abstract
研究领域:
分析化学
·
药理学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Yue Wang,Wenchang Zhao,Xuemeng Tian,Huijia Song,Ruixia Gao,Xiaoshuang Tang,Xiaojing Zhang,Yi Hao,Yuhai Tang
Hydrophilic molecularly imprinted polymer for effective removal of pollutants from environmental water has attracted much attention due to their high selectivity and matrix compatibility. Herein, resorcinol and formaldehyde have been first proposed as the hydrophilic monomers to form water-compatible molecularly imprinted resin magnetic nanomaterials (Fe 3 O 4 -RF-MIPs) by adopting a two-step template immobilization strategy of surface molecular imprinting technique. In the synthesis process, carboxyl-modified Fe 3 O 4 nanoparticles and sulindac were adopted as carrier and template molecule, respectively. The physical and chemical properties, preparation process, and adsorption conditions of Fe 3 O 4 -RF-MIPs were investigated in detail. Fe 3 O 4 -RF-MIPs exhibit uniform morphology, high crystallinity, outstanding magnetic property, fast binding kinetics, high adsorption capacity, satisfactory selectivity, and excellent reusability. Meanwhile, combining with HPLC, Fe 3 O 4 -RF-MIPs as solid phase extraction adsorbents have been successfully applied to specifically enrich and detect sulindac in sewage water. Accordingly, Fe 3 O 4 -RF-MIPs with ordered and evenly distributed imprinted sites and excellent dispersibility in water would be potential in the field of water environmental pollution control.
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DOI
高影响因子
危险品化学品经营许可证（带存储）