npj Science of Food
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2025
·Vol. 9
·期 1
·1-10
·
IF 7.8
Zhao Huamin,Zheng Xiaoxi,Su Jihao,Wang Jun,Wang Xinrui,Li Hang,Wang Juan,Shen Kexin,Zhao Chao
Electrochemical detection of foodborne pathogens requires simplified electrode modification and enhanced sensitivity. This study introduced Cd-decorated ternary FeCoNi layered double hydroxides (Cd-FeCoNi LDHs) platform for label-free Staphylococcus aureus ( S. aureus ) detection via Cu 2+ biosorption. The FeCoNi LDHs were engineered with optimized metal sites to create lamellar structures with high electrocatalytic surface area. Further incorporating with Cd particles deposition, the response of the Cd-FeCoNi LDHs to Cu 2+ exhibited a double-enhanced differential pulse voltammetry signal. Concurrently, S. aureus has been explored to adsorb Cu 2+ through surface carboxyl and phosphate groups. By integrating with specific magnetic separation, the variation of Cu 2+ derived from biosorption by S. aureus triggered the rapid electrical response of the Cd-FeCoNi LDHs platform. This approach achieved 5.4 CFU/mL detection without bio-labeling, while resisting matrix interference. This work highlighted the innovative application of bacterial metal ions biosorption and label-free sensing platform for foodborne pathogen detection.
研究领域:
生物电化学
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材料科学
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微生物检测
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Gaohao Liao,Tingting Yang,Zhenzhen Xu,Jiamei Wang,Guanghua Xia,Xueying Zhang,Haohao Shi,Liming Zhang
The resilient biofilms formed by Staphylococcus aureus ( S. aureus ) on food and food-processing surfaces pose serious risks of persistent contamination, product spoilage, and public health threats. This highlighted the urgent need for safe, nontoxic, and highly efficient antibiofilm agents. In this study, plasma-activated lactic acid (PALA) was evaluated for its efficacy in eradicating S. aureus biofilms and its underlying mechanism of action. Notably, a 20 min PALA treatment reduced viable biofilm cells by 4.10 log CFU/mL, increased propidium iodide fluorescence by 299.56%, and elevated supernatant OD₂₆₀/OD₂₈₀, indicating massive membrane disruption and intracellular leakage. Flow cytometry confirmed that 68.50% of cells suffered membrane damage. Moreover, PALA further induced severe oxidative stress, with intracellular ROS levels increasing by 163.09% after 15 min of treatment. During the 0–20 min treatment period, ATP levels in biofilm cells showed a transient increase from 0.094 to 0.79 μmol/L before declining, reflecting metabolic disruption. PALA exerted its antibiofilm effect through multiple synergistic mechanisms: structural disintegration of the biofilm matrix, severe membrane damage with leakage of intracellular components, significant ROS accumulation causing metabolic disruption and double-stranded DNA breaks, and reduction in secondary biofilm formation by impairing auto-aggregation, lowering zeta potential, and decreasing surface hydrophobicity. Collectively, these findings demonstrate that PALA can effectively remove S. aureus biofilms and represents a promising novel biofilm control agent.
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DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Yi Guo,Chang’e Wang,Lili Zhang,Ying Lu
The residual tetrodotoxin (TTX) in discarded pufferfish viscera poses environmental and safety risks. However, current TTX removal technologies suffer from low efficiency, long processing time, and difficulty in separating adsorbents from toxins. To address this, we developed an efficient and convenient removal method by constructing an Staphylococcus aureus peptidoglycan-labeled magnetic bead probe (S-PG-MB) from Staphylococcus aureus peptidoglycan (S-PG) and carboxylated magnetic beads (MB). Using 1.0 μm MB at an MB-to-S-PG ratio of 1:2, activation with 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC) (20 mg/mL) for 45 min, and coupling in pH 6.5 sodium acetate buffer for 105 min, the resulting probe achieved up to 95% TTX removal at 37°C within 35 min—more than twice the efficiency of S-PG alone. Toxicity evaluation using SH-SY5Y neuronal cells and a mouse bioassay further confirmed that the S-PG-MB probe reduced TTX neurotoxicity by approximately 97%. It also effectively removed TTX from the internal organs of perch, with a removal rate of 73%, and maintained stable performance over five reuse cycles (< 4% efficiency loss). Kinetic and isotherm modeling indicated monolayer adsorption that was largely temperature-independent. The high performance was attributed to the uniform distribution on the probe surface, enabling rapid chemisorption. In summary, the S-PG-MB probe combines operational simplicity, high capacity, and reusability, offering a promising idea and approach for developing novel adsorbents and advancing efficient removal technologies for TTX and other marine biotoxins.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Luting Yan,Yuhao Zhang,Chao Wang,Lei Tian,Zekun Ren,Xia Sun,Yemin Guo,Falan Li,Qingqing Yang,Yanyan Zhang
This study presented a dual-mode biosensor that integrates electrochemiluminescence (ECL) and colorimetric methods for detecting Staphylococcus aureus ( S. aureus ) in seafood. The biosensor utilized self-enhanced gold nanoclusters (DPEA-AuNCs) as luminescent materials, which exhibited positive potential peaks without coreactant, effectively reduced the instability. Additionally, DPEA-AuNCs exhibited enzyme-like activity, catalyzing the substrate 3′ 3′ 5′ 5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce blue reaction products for visual detection. DPEA-AuNCs were immobilized on magnetic iron tetraoxide (Fe 3 O 4 ) nanoparticles via aptamer, enabling solid-liquid separation by magnetic field. The precipitate was utilized for ECL detection, while the supernatant was employed for absorbance measurements. When S. aureus bound to the aptamer, the DPEA-AuNCs detached from Fe 3 O 4 , resulting in a poor ECL signal intensity. Meanwhile, the DPEA-AuNCs in the supernatant catalyzed the TMB substrate, resulting in color change to blue. A Microsoft mini-program was developed to analyze the correlation between RGB values and S. aureus concentration for rapid detection. The biosensor demonstrated low detection limits of 1.78 CFU/mL and 2.35 CFU/mL for ECL and colorimetric modes, respectively. This method exhibited detection sensitivity and accuracy while simplifying operations, offering an intuitive and efficient approach to food safety detection.
研究领域:
生物传感器
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食品安全
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分析化学
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纳米生物技术
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微生物检测
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DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·39617
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IF 3.9
Jiang Yourang,Tang Xueyong,Wang Ailin,Yang Hua,Jiang Xue,Zhang Yinxin,Lou Fanglu
Antimicrobial resistance (AMR) poses a critical global health challenge, with an estimated 1.27 million AMR-attributable deaths in 2019 and projections of 39 million cumulative deaths from 2025 to 2050, particularly driven by Staphylococcus aureus and methicillin-resistant strains (MRSA) that form robust biofilms conferring up to 1000-fold antibiotic tolerance and complicating hospital-acquired infections. Here, we report a green, one-pot synthesis of shikonin-copper nanoparticles (SCu NPs), employing shikonin (SK)—a naphthoquinone from Lithospermum erythrorhizon roots-as a dual chelator and stabilizer, without exogenous reductants or surfactants; density functional theory (DFT) computations guide the design, predicting thermodynamically favored 1:2 Cu(II): SK stoichiometry, yielding stable spherical nanoparticles (39.25 ± 3.24 nm) with preserved Cu(II) oxidation state, as validated by TEM, XPS, XRD, and UV-Vis spectroscopy. SCu NPs exhibit potent antibacterial activity against S. aureus ATCC 25,923, with minimum inhibitory and bactericidal concentrations (MIC/MBC) of 4/8 µg/mL—half those of SK (8/16 µg/mL)—and rapid bactericidal kinetics, reducing viability by 66% within 2 h; antibiofilm assays reveal concentration-dependent inhibition, achieving up to 89% biomass reduction at 32 µg/mL, outperforming SK, CuSO 4 , and their mixture, with SEM confirming extensive membrane disruption and cytoplasmic leakage. This synergy arises from Cu(II)-mediated reactive oxygen species (ROS) generation, enhanced lipophilicity, and SK’s quorum-sensing inhibition, positioning SCu NPs as a sustainable, multi-target platform integrating natural product chemistry and nanotechnology to combat biofilm-associated AMR, mitigate resistance emergence, and advance therapeutics for recalcitrant infections.
研究领域:
纳米技术
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抗菌治疗
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材料科学
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药物纳米技术
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传染病
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Baoqing Zhou,Qinghua Ye,Fan Li,Xinran Xiang,Yuting Shang,Chufang Wang,Yanna Shao,Liang Xue,Jumei Zhang,Juan Wang,Yu Ding,Moutong Chen,Qingping Wu
Rapid, accurate point-of-care testing (POCT) for pathogenic bacteria detection is the key for avoiding foodborne diseases caused by pathogens or their toxins. The lateral flow biosensor (LFB) based on clustered regularly interspaced short palindromic repeats (CRISPR/Cas) has displayed remarkable potential for pathogen diagnosis. In this study, we report a novel CRISPR/Cas12a-based fluorescence enhanced LFB in conjunction with functionalized quantum dots , combined with recombinase-assisted amplification (RAA), to establish low-cost, simple, and sensitive detection of Staphylococcus aureus , namely CRA-LFB (CRISPR/Cas-recombinase-assisted amplification based LFB). The CRA-LFB assay is characterized by Cas12a-mediated trans-cleavage activation induced by the target DNA to digest biotin-DNA probes, caused no complementarity to the capture probe immobilized on the test (T) line, and resulted in an undetectable T line fluorescence signal on LFB. The naked eye or fluorescence strip reader was used to determine the fluorescence intensity of the T and control lines. The limit of detection (at optimal conditions) was as low as 75 aM of genomic DNA, and 5.4 × 10 2 cfu/mL of S. aureus in pure cultures were detected. Moreover, this CRA-LFB assay can rapidly and accurately detect S. aureus in spiked and natural meat and vegetable samples. The CRA-LFB method yielded high specificity and no interference from other nontargeted bacteria. The results exhibited high-resolution, high-intensity fluorescent signals obtained within 70 min. This bacterial detection platform is simple, low cost, almost equipment-free, and fully qualified for food and clinical diagnosis onsite testing requirements.
研究领域:
生物技术
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微生物学
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食品安全
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即时诊断
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DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2021
·Vol. 37
·期 12
·1-9
·
IF 3.312
Wu Chuan,Zeng Yuanyuan,He Yang
Staphylococcus aureus is a common clinical bacterial pathogen that can cause a diverse range of infections . The establishment of a rapid and reliable assay for the early diagnosis and detection of S. aureus is of great significance. In this study, we developed a closed-tube loop-mediated isothermal amplification (LAMP) assay for the visual detection of S. aureus using the colorimetric indicator hydroxy naphthol blue (HNB). The LAMP reaction was optimized by adjusting the amplification temperature, the concentrations of Mg 2+ , dNTP, and HNB, and the incubation time. In the optimized reaction system, the specificity of LAMP for S. aureus was 100%. The results established that this method accurately identified S. aureus , with no cross-reactivity with 14 non- S. aureus strains. The limit of detection (LOD) of LAMP was 8 copies/reaction of purified plasmid DNA or 400 colony-forming units/reaction of S. aureus . Compared with conventional PCR, LAMP lowered the LOD by tenfold. Finally, 220 clinically isolated strains of S. aureus and 149 non- S. aureus strains were used to evaluate the diagnostic efficacy of LAMP (test accuracy, 99.46%). The findings indicated that LAMP is a reliable test for S. aureus and could be a promising tool for the rapid diagnosis of S. aureus infections.
研究领域:
微生物学
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分子生物学
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临床诊断
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DOI
PubMed
高影响因子
TALANTA
·
2024
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IF 5.6
Mimi Sun,Chongbo Ma,Mohammed Y. Emran,Ahmed Kotb,Jing Bai,Ming Zhou
The advanced devices that function fully without the need for external accessories are regarded as a pinnacle goal in the design and construction of modern ones. Staphylococcus aureus ( S. aureus ), a prominent human pathogen, is responsible for causing a wide variety of infections and chronic diseases. Herein, we present the first instance of a fully integrated wireless microfluidic immunosensing system (FIWMIS) capable of conducting point-of-care S. aureus monitoring in real samples of S. aureus -spiked commercial purified drinking water and S. aureus -spiked watermelon juice. The development of the proposed FIWMIS became a reality by conquering significant engineering hurdles in seamlessly integrating a microfluidic unit for liquid sample transport without the need of an external pump, an immunosensing unit for S. aureus monitoring, and an electronic control unit for signal conversion and wireless transmission. Such full integration culminated in a FIWMIS that upholds its pump-free, wireless, and low-cost characteristics for portable monitoring of S. aureus .
研究领域:
生物医学工程
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微生物学
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传感器技术
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产品关联
DOI
PubMed
高影响因子
Infection and Drug Resistance
·
2018
·
IF 3.443
Yufeng Du,Li Liu,Chunping Zhang,Yani Zhang
Staphylococcus aureus is becoming increasingly intractable because of its ability to acquire antimicrobial resistance and secrete numerous virulence factors that can exacerbate inflammation. Alpha-hemolysin (Hla) is a pore-forming virulence factor produced by S. aureus that can self-assemble into heptameric mushroom-structured pores in target cell membranes, leading to cell lysis and death. In the present study, we sought to better understand the mechanism underlying hemolysis and the oligomerization of Hla by creating nine mutants with single amino acid changes in different positions of the Hla protein: N17C, T18C, P103C, N105C, M113C, T117C, N121C, D128C, and T129C. The results showed that the P103C and N105C mutations, which are located in the triangle region, significantly diminished hemolysis and heptamer formation when compared with the wild-type Hla protein. This suggests that the P103 and N105 residues play key roles in the assembly of the Hla pore. These results improve our understanding of the mechanism underlying the pore-forming ability of Hla.
研究领域:
微生物学
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分子生物学
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感染与免疫
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DOI
PubMed
高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2019
·Vol. 31
·期 4
·811-821
·
IF 2.125
Ali Shujat,Perveen Samina,Ali Mumtaz,Shah Muhammad Raza,Khan Ezzat,Sharma Arumugam Selva,Li Huanhuan,Chen Quansheng
In recent era bacteria that were previously susceptible to different antibiotics are getting tolerant to these drugs. Novel nanoantibiotics can evade these emerging bacterial resistances. Thus, cefadroxil was conjugated to silver and gold nanoparticles (NPs) and resultant nanoconjugates (i.e., Cefd-AgNPs and Cefd-AuNPs) were examined against staphylococcus aureus ATCC 11632. Devised nanoconjugates were characterized by applying diverse range of characterization tools, including UV–visible, IR spectroscopy, scanning electron microscopy and atomic force microscopy (AFM). Moreover, bactericidal potential of Cefd-AgNPs and Cefd-AuNPs was compared with the efficacy of non-conjugated cefadroxil served as control. Experimentally obtained results show that conjugation of cefadroxil with gold and silver NPs enhanced the antibacterial potential of cefadroxil up to 3 and 2 times, respectively. Surface topography of the controlled and treated bacterial cells was also scanned by AFM. Moreover, AFM revealed that effective treatment time of cefadroxil (i.e., 8 h) also get reduced to one half upon conjugation of cefadroxil with both gold and silver (i.e., 4 h).
研究领域:
药学
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纳米技术
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微生物学
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DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2024
·
IF 3.6
Yunjing Jia,Yuli Li,Mingzhu Wang,Fuyou Wang,Qingmin Liu,Zhimei Song
Considering cellular uptake promotion of lecithin and high expression of phospholipase in S. aureus, we designed curcumin (Cur)-loaded soy lecithin-based mPEG-PVL copolymer micelles (MPPC). The effect of soy lecithin on the anti-S. aureus activity of the formulation was studied with cur-loaded mPEG-PVL micelles (MPC without soy lecithin) as control. It was found that MPPC enhanced the water-solubility of Cur, and showed slow and sustained release behavior of Cur. Although MPPC had the same anti-S. aureus activity as Cur, its activity was significantly higher than MPC due to the cellular uptake promotion of soybean lecithin. It was noted that MPPC had good inhibition or destruction effect on biofilm, significant cell membrane damage, strong inhibition effect on protease or lipase production, and obvious induction effect on ROS expression when compared with Cur and MPC. So, the introduction of soy lecithin could improve the antibacterial activity of Cur. The lecithin-based micelles would offer potential to deliver antibacterial drugs for improved therapeutic action.
研究领域:
药剂学
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微生物学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Liu Zhi-Hai,Wang Wei-Mei,Zhang Zhen,Sun Liang,Wu Shuai-Cheng
The emergence and spread of antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), underly the urgent need to develop novel antibacterial drugs. Macleaya cordata, a traditional medicinal plant, has been widely used in livestock animals, plants, and humans. Alkaloids are the primary bioactive compounds of Macleaya cordata and exhibit antibacterial, antiinflammatory, and antioxidant activities. Nevertheless, the antibacterial compounds and mode of action of Macleaya cordata remain unclear. In the present study, we investigated the antibacterial activity and mode of action of alkaloids from Macleaya cordata. Sanguinarine, 6-ethoxysanguinarine (6-ES), 6-methoxydihydrosanguinarine (6-MS), chelerythrine (CH), and dihydrochelerythrine (DICH) exhibited good antibacterial activity against Gram-positive bacteria, including MRSA. 6-ES rapidly killed MRSA, possibly by interfering with membrane and metabolic functions including ROS production by targeting the membrane and FtsZ in S. aureus. Additionally, 6-ES directly suppressed the hemolytic activity of α-hemolysin, alleviated inflammatory responses, and eliminated intracellular MRSA, as well as displayed low development of drug resistance, in vitro. Finally, a 6-ES-loaded thermosensitive hydrogel promoted wound healing in mice infected with MRSA. These results supported 6-ES as a novel potential candidate or leading compound with antibacterial, antivirulence, and host immunomodulatory activities in fighting against bacterial infections.
研究领域:
药理学
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微生物学
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传染病学
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植物科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）