LANGMUIR
·
2017
·
IF 3.833
Zhe Qin,Ying Sun,Baohuan Jia,Dan Wang,Yan Ma,Gang Ma
Thioflavin T (ThT) is widely used as a fluorescent probe for amyloid fibril detection. Yet the exact kinetic mechanism of ThT binding onto amyloid fibril remains elusive. Previously reported kinetic studies using ThT-fluorescence-detected kinetic design suggested two completely different ThT-binding mechanisms. In one study, a multistep sequential binding mechanism onto a single ThT-binding site was suggested. In another study, a one-step parallel binding mechanism onto multiple ThT-binding sites was suggested. The discrepancy is likely due to the incapability of ThT-fluorescence-detected kinetic design to differentiate the two above-mentioned mechanisms. Considering the weakness of the ThT-fluorescence-detected approach, we investigated the ThT-binding mechanism onto the amyloid fibril of hen egg white lysozyme (HEWL) using a new approach, ThT-absorbance-detected kinetic design. Our new results suggest that ThT binds to HEWL fibril through the one-step parallel binding mechanism. We hope our work can offer some new insights into the interactions between dye molecules and amyloid fibrils.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2017
·Vol. 164
·期 4
·A820
·
IF 3.259
Junming Zhang,Hao Chen,Xiaowen Sun,Xiaojuan Kang,Yan Zhang,Chaohe Xu,Yuxin Zhang
Li-ion hybrid electrochemical capacitors (Li-HECs) with higher energy density than electric double layer capacitors (EDLC) and larger power density than current rechargeable batteries are attractive candidates in future energy related applications. However, the electrochemical properties of current Li-HECs are greatly restricted by its Li-ion insertion type of electrode materials. Herein, we report an efficient approach to prepare one dimensional T-Nb2O5 nanowires growth on activated carbon cloth as freestanding hybrid electrodes for Li-HECs. The T-Nb2O5 nanowires with diameter of 20∼50 nm and length of several micrometers cross with each other and form a three dimensional (3D) porous network. The designed hybrid electrodes can deliver a gravimetric capacitance as high as 220 mAh g−1 at current density of 0.5 C and advanced cycle life with capacity retention of 86% after 1000 cycles at 5 C. The outstanding electrochemical performances are mainly attributed to the reduction in the lithium ion diffusion length brought by the crossed porous structure. And the enhancement of electron transportation enjoys the benefit from the in-situ growth of T-Nb2O5 nanowires on carbon cloth and the binder-free electrode structure.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2013
·Vol. 181
·期 1
·155-162
·
IF 3.434
Wang Liheng,Liao Xiaolei,Ding Yingtao,Gao Fei,Wang Qingxiang
Abstract We report on an electrochemical DNA biosensor consisting of a glassy carbon electrode modified with a film of electropolymerized Eriochrome Black T (pEBT) that serves as a functional platform for the immobilization of probe DNA. pEBT was deposited via cyclic voltammetry, and the amino-modified DNA capture probe was covalently linked to the surface via a sulfanilamide coupling reaction. The single step of the assembly process was monitored by atomic force microscopy and electrochemistry. The surface density of DNA probe on the biosensor interface was calculated to be 1.7 × 10 −10 mol cm −2 using methylene blue as an electroactive probe. Hybridization experiments showed the peak currents of methylene blue to decrease with increasing concentration of complementary sequence in the range from 5.0 f. to 5.0 pM. The detection limit is as low as 0.11 fM. Selectivity studies showed that the biosensor can discriminate a fully complementary sequence from a single-base mismatch, three-base mismatch, and a fully non-complementary sequence. The biosensor displays good stability and can be regenerated due to the beneficial effects of electropolymerization and covalent immobilization of probe DNA.
A novel DNA biosensor was fabricated through a facile sulfamide coupling reaction between probe DNA and eriochrome black T that electropolymerized on a glassy carbon electrode
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2017
·Vol. 27
·期 45
·1703934
·
IF 12.124
Cong Li,Hui Du,Aizhen Yang,Shuaibing Jiang,Zhenhua Li,Dan Li,John L. Brash,Hong Chen
Surface modification with bioactive agents capable of combating thrombosis is a widely used strategy for developing antithrombotic biomaterials. However, exposure of the blood to the antithrombotic agent on the material surface may cause hemostatic disorders under normal conditions. Ideally an implanted biomaterial should respond appropriately on demand to a specific change in the physiologic environment, as happens in the body itself. In the present study, a thrombosis-responsive surface coating with the ability to lyse fibrin as it forms is reported. The coating consists of nanocapsules (NCs) in which the fibrinolysis activator t-PA is encapsulated in a thrombin-degradable hydrogel shell. The t-PA NCs are attached to several materials covalently through a polydopamine adhesive layer. The resulting surfaces are treated with the antifouling agent glutathione (GSH) to prevent further interactions with blood/plasma components. The t-PA NCs/GSH-coated surface is stable and remain inert in normal plasma environment while releasing t-PA and promoting fibrinolysis when thrombin is present. The fibrinolytic activity increases with increasing thrombin concentration, and therefore presumably with the extent of thrombosis. This work constitutes the first report of an antithrombotic coating whose function is triggered and regulated, respectively, by the appearance of thrombin and the extent of coagulation.
研究领域:
生物材料
·
医学工程
·
血液学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Immunology
·
2018
·
IF 5.511
Chen Haiming,Liu Huazhen,Lu Chuanjian,Wang Maojie,Li Xiong,Zhao Hui,Yan Yuhong,Yu Wanling,Han Ling,Dai Zhenhua
Psoriasis is an autoimmune and inflammatory disease, which is estimated to affect 2–3% of the population in the world. PSORI-CM02 is an empirical formula of Chinese medicine optimized from Yin Xie Ling, which is widely used to treat psoriasis in China for decades. However, its antipsoriatic mechanisms are still not well understood. Here, we explored the therapeutic effects of PSORI-CM02 on psoriasis and its mechanisms of action in imiquimod-induced psoriasis-like mouse models and human HaCaT cells. In experiments in vitro, PSORI-CM02 significantly inhibited HaCaT cell proliferation in dose-dependent and time-dependent manners. Furthermore, it hindered the progression of HaCaT cell cycle and arrested HaCaT cells at G1 phase. On the other hand, our in vivo studies demonstrated that PSORI-CM02 dramatically reduced psoriasis area and severity index scores and lesion temperature in imiquimod-induced psoriatic mice. The antioxidative activities of glutathione, catalase, and superoxide dismutase were increased while oxidative activity of malonaldehyde was markedly decreased after treatments with PSORI-CM02. PSORI-CM02 also suppressed the mRNA expression of proinflammatory cytokines, including TNF-α, IL-6, and IL-17, and lowered their protein levels in the serum as well. In addition, PSORI-CM02 could reduce the expression of IKKα and NF-κB in psoriatic skin tissue. It also upregulated the proportion of CD4+ Foxp3+ regulatory T cells (Tregs) in both lymph nodes and spleens and promoted CD4+ CD25+ Treg proliferation in vitro. Taken together, our research demonstrated that PSORI-CM02 inhibited HaCaT cell proliferation by arresting them at G1 phase and alleviated systemic inflammation and psoriasis in mice via altering the oxidative/anti-oxidative status, tipping the balance between Th17 responsiveness and CD4+ Foxp3+ Treg generation, and suppressing the expression of proinflammatory cytokines as well as NF-κB signaling.
研究领域:
免疫学
·
药理学
·
皮肤病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Imran Mahmood Khan,Sen Zhao,Sobia Niazi,Ali Mohsin,Muhammad Shoaib,Nuo Duan,Shijia Wu,Zhouping Wang
T-2 is a trichthecenes mycotoxin responsible for severe food poisoning and toxic influences in humans and animals. Therefore, a rapid and sensitive detection of T-2 is an urgent need to protect the public health mainly caused by the consumption of contaminated agricultural products. Herein, we have prepared one pot synthesis of aptamer functionalized silver nanoclusters (apt-AgNCs) for fluorescent detection of T-2 that relies on novel fluorescence resonance energy transfer (FRET). Apt-AgNCs, act as energy donor, were applied as fluorescent sensing probe whose fluorescence was quenched by MoS 2 employed as fluorescent acceptor. Introduction of target in proposed bioassay leads to desorption of Apt-AgNCs from MoS 2 , and recovery of fluorescence in a toxin concentration dependent-manner. A dynamic quantification range was 0.005–500 ng mL −1 while the LOD was 0.93 pg mL −1 . Spiking analysis reveals the efficiency and robustness of the developed assembly, with recovery percentages ranged from 89.46% to 102.08% and 90.41% to 107.75% in maize and wheat samples, respectively. The results were cross-verified using standard commercial T-2 EISA kit. In conclusion, developed bioassay may find good utility in testing for risk assessment of T-2 toxin.
研究领域:
食品安全
·
生物传感
·
毒理学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Yuanyuan Dai,Ying Zhang,Wanliang Liao,Weiping Wang,Liang Wu
A label-free fluorescence aptasensor was developed for the rapid detection of tetracycline (TET) based on G-quadruplex structure of TET aptamers and G-quadruplex specific dye Thioflavin T (ThT). The fluorescence of free ThT is essentially weak in aqueous solution, whereas it selectively identifies the G-quadruplex of aptamers to form the G-quadruplex/ThT conjugates, resulting in an enormous increase of the fluorescence intensity . However, the fluorescence intensity of G-quadruplex/ThT conjugates was drastically suppressed due to the release of free ThT from G-quadruplex/ThT conjugates after the addition of TET via specific binding with TET aptamers. The key factors affecting sensitivity and selectivity including the reaction medium, binding time of ThT to TET aptamers, incubation time between TET aptamers and TET, concentration of ThT and TET aptamers were investigated in detail. The optimal conditions were as follows: ultrapure water as reaction medium, binding time of 5 min, incubation time of 1 min, 9.0 μmol/L ThT and 0.03 μmol/L aptamers. A good linear relationship (correlation coefficient of 0.9973) was obtained between the fluorescence quenching efficiency ( F 0 − F ) / F 0 and the logarithm of TET concentration in the range of 0.01–1.0 μmol/L. The limit of detection was 0.001 μmol/L (S/N = 3). The proposed assay was applied for the detection of TET in the spiked honey and milk samples with recoveries ranging from 93.5% to 106.9%. The developed label-free fluorescence aptasensor showed advantages of high specificity, low cost and short time-consuming, illustrating potential application for on-site detection of TET in foodstuffs.
研究领域:
分析化学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Piao Liu,Jianxin Cao,Zheng Xu,Chunliang Yang,Xiaodan Wang,Fei Liu
Core-shell structured ZSM-5@t-ZrO 2 catalysts were prepared by a hydrothermal coating method, and their physicochemical properties, catalytic performance, and deactivation behavior in thiolation reactions were studied using a ZSM-5/t-ZrO 2 physically-blended catalyst as a comparison. Reactions using the core-shell ZSM-5@t-ZrO 2 catalyst were carried out at a pressure of 0.7 MPa, 370 °C, a H 2 S:CH 3 OH molar ratio of 2:1, and a N 2 flow rate of 90 ml·min −1 . The conversion of CH 3 OH, and the selectivity and yield of CH 3 SH under these conditions were 92.07%, 90.89%, and 83.68%, respectively, and the catalytic lifetime was longer than 30 h. Compared with the ZSM-5/t-ZrO 2 physically-blended catalyst, the CH 3 SH yield and the catalytic lifetime of the core-shell structured ZSM-5@t-ZrO 2 catalyst were higher by 5.37% and 150%, respectively. The unique reaction pathway of the shell (t-ZrO 2 , Lewis (L) base active site)-core (ZSM-5, Brǿnsted (B) acid active site) and the Lewis-Brønsted acid active center based on a composite solid-solution interface were mainly responsible for the high activity of the thiolation reaction. Meanwhile, the formation of a micro-mesoporous system increased the diffusion of molecules and reduced the deactivation rate of carbon- and sulfur-containing deposits. Generally, carbon- and sulfur-containing substances deposited on catalysts were mainly composed of polymethylbenzene, polymethyl naphthalene, cyclic elemental sulfur, and other compounds containing C S and S S bonds. Finally, the core-shell structured catalysts showed excellent resistance to deactivation by carbon- and sulfur-containing deposits.
研究领域:
化学工程
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
Applied Materials Today
·
2023
·
IF 8.3
Weimin Yin,Jiao Chang,Jiuyuan Sun,Yuge Zhao,Shiyu Chen,Hui Zhi,Jie Zang,Tingting Zhang,Yongyong Li,Haiqing Dong
Poor efficacy in solid tumor immunotherapy mainly ascribed to few CD8 + T cells infiltration with low activity in tumor immunosuppressive microenvironment (TIME). Ferroptosis can be harnessed for activating system immune response for more CD8 + T cells infiltration. However, the activity of infiltrated CD8 + T cells will be heavily weakened by the nutrient barrenness and immunosuppressive microenvironment, which formidably impaired the biologically closed-loop reciprocal process between active CD8 + T cells and cellular ferroptosis. Considering the above two key influence factors, we herein elaborately labeled l -Arginine (Arg) with 3,4-dihydroxybenzaldehyde (PA) via reversible Schiff-base bond, and then drive nanoassembly of PA-Argby iron ions mediation (Fe@PA-Arg NPs). Iron released from Fe@PA-Arg NPs in acidic tumor environment could produce reactive oxygen species (ROS) and thus successfully induce immunogenic ferroptosis of cancer cells. Subsequently, promoting the dendritic cells maturation and CD8 + T cells infiltration. Meanwhile, ROS could also induce the lipid peroxidation of tumor associated macrophages that contributed to mitochondrial dysfunction, thereby repolarized M2 to M1 phenotype to relieve the immunosuppression. Arg released from Fe@PA-Arg NPs as nutrient supply to restore infiltrated T cells’ activity by combining Arg metabolic adaption with immunosuppressive mitigation. Reciprocally, restored activated CD8 + T cells could secrete high levels of Interferon-γ, which in turn enhances ferroptosis, thereby successfully initiating and forming a closed-loop, and the tumor inhibition rate of 63.5% was achieved. This study highlights a novel and facile strategy to improve the immunotherapeutic efficiency of murine 4T1 solid tumors.
研究领域:
癌症免疫疗法
·
纳米技术
·
免疫学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS
·
2022
·
IF 3.027
Hua Jiao,Jiechen Jin,Kang Zhao,Xue R Zhou,Xinyuan Zhang,Sen Song,Jia Wang,Yufei Tang,Guodong Cui
Barium titanate (BaTiO3) is a typical perovskite-type oxide with high dielectric constant, low dielectric loss, and excellent ferroelectric, piezoelectric, pyroelectric, and other properties. In view of the low photogenerated electron-hole separation efficiency of T-BT itself, it is proposed to prepare nanocomposites with good catalytic performance by compound modification with Ag. Considering repeated use, on the basis of powder catalysis, a porous block is prepared by compounding powder and organic polymer PVDF. In this study, the t-BaTiO3/Ag/β-PVDF composite material with porosity of ∼70.4% was prepared with the raw material of PVDF and piezoelectric t-BaTiO3/Ag. The mechanical and piezoelectric photocatalytic properties of the composite material was studied systematically. The piezoelectric coefficient d33 is 1.44 pC/N which can be explained by COMSOL simulation calculation. Moreover, the compressive strength is 1.76 MPa. The degradation MO and RhB results indicated that t-BaTiO3/Ag/β-PVDF composite material had good degradation and cycle stability.
研究领域:
材料科学
·
催化化学
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Qian Li,Yue Xu,Wei He,Yuan Fang Li,Peng Fei Gao,Cheng Zhi Huang
Core-shell materials have superior properties over their single-component counterparts, and thus how to develop a new type of core-shell structure deserves much hard work. Herein, a plasmon-dependent photophysical (PDPP) process was developed to prepare [email protected] T core-shell nanostructures with reversible properties, wherein Safranine T (ST) was at first adsorbed on the surface of negatively charged gold nanospheres (AuNSs) through the electrostatic attraction and then controllably deposited by adjusting plasmonic illumination. The whole PDPP process was monitored in real-time via dark field microscopy (DFM) imaging system, verifying that plasmon-dependent light-matter interaction occurs. The as-prepared [email protected] nanostructures were then successfully applied for a reversible process of edit and erase that was suitable for encryption application with nanosized precision. This research showed that the PDPP process has high promise for developing precise structures of new materials, particularly those through nanoprocessing and nanooperation.
研究领域:
纳米材料科学
·
光学工程
·
材料化学
✓ 已验证
产品关联
DOI
高影响因子
ARCHIVES OF DERMATOLOGICAL RESEARCH
·
2025
·Vol. 317
·期 1
·1-16
·
IF 2.1
Lin Ling,Li Wei,Gao Xinjing,Li Qian,Zhou Xin,Liu Weiyu,Zhong Xuelian,Yang Yunqing,Zhang Xibao,Luo Quan
Psoriasis represents a chronic inflammatory skin disease occurring globally. We investigated the role of plasma exosomal microRNA (miR)- 148a- 3p and its target gene Bim in psoriasis. Plasma exosomes (Exos) and CD4 + T cells were extracted from psoriatic patients. miR- 148a- 3p expression in Exos and CD4 + T cells of psoriatic patients, the proportions of CD4 + T cell subsets, and the contents of anti-inflammatory/pro-inflammatory factors were determined by RT-qPCR, flow cytometry and ELISA. The correlation between plasma exosomal miR- 148a- 3p and CD4 + T cell subsets in psoriatic patients was analyzed by Pearson’s analysis. The psoriatic mouse was treated with Exos/antagomir miR- 148a- 3p. Histopathological changes in the skin were observed. The CD4 + T cell subset levels, serum cytokine contents and miR- 148a- 3p expression in the blood were measured. The miR- 148a- 3p-Bim targeted binding relationship was predicted and verified by Starbase database and dual-luciferase assay. The Bim expression in psoriatic mice was determined. Psoriatic patients had highly-expressed miR- 148a- 3p in both Exos and CD4 + T cells, and abnormal CD4 + T cell subsets and cytokine levels. Plasma exosomal miR- 148a- 3p was correlated with the CD4 + T cell subsets in psoriatic patients. Exos caused down-regulated miR- 148a- 3p level in skin tissues of mouse, regulated CD4 + T cell function and aggravated the symptoms in psoriatic mice. miR- 148a- 3p repression partially reversed the role of Exos in CD4 + T cell function and psoriasis-like symptoms. Exos-carried miR- 148a- 3p targeted Bim in CD4 + T cells of psoriatic mice. Plasma exosomal miR- 148a- 3p targeted Bim to affect the dysfunction of CD4 + T cells in psoriatic mice, thereby aggravating the psoriasis-like symptoms.
研究领域:
皮肤病学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）