Food Science and Human Wellness
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2020
·
IF 2.455
Ting Zhang,Mengzhen Ding,Xichang Wang,Jian Zhong
The droplet and creaming stability of food emulsions stabilized by mixed emulsifiers is a research hotspot in the field of emulsions. In this work, we mainly explore the effect of pH change on the droplet and creaming stability of fish oil-loaded emulsions synergetically (Span 80 and SL) or competitively (Tween 80 and SDS) stabilized by gelatin/surfactant. The results demonstrate that initial droplet stability and droplet storage stability, and creaming stability of the pH-adjusted emulsions are dependent on both the adsorption ways of emulsifiers and the adjusted pH: (1) Competitively stabilized emulsions have more stable droplets than synergetically stabilized emulsions; (2) SDS-dominant competitively stabilized emulsions have more stable droplets than gelatin-dominant emulsions; (3) Basic pH-adjusted emulsions have more stable droplets than acidic pH-adjusted emulsions; (4) The synergetically stabilized emulsions at acidic pH have significantly higher creaming indexes than that at basic pH; and (5) The competitively stabilized emulsions have high or similar creaming indexes to that at acidic pH. Further, the mechanisms are proposed according to Stokes’ Law. This work will provide useful information to understand the interfacial properties of mixed emulsifiers in the food emulsions and promising application perspective for the development of food beverages including acidic and alkali beverages.
研究领域:
食品科学
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化学工程
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胶体科学
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2018
·Vol. 7
·期 22
·1800830
·
IF 5.609
Li Song,Xinghua Dong,Shuang Zhu,Chunfang Zhang,Wenyan Yin,Xiao Zhang,Xiangfeng Liu,Zhanjun Gu
Although various types of photothermal agents are developed for photothermal cancer therapy, relatively few photothermal agents exhibit high tumor inhibition rate under relatively mild conditions. Herein, a multifunctional Bi 2 S 3 –Tween 20 nanoplatform loaded with PI3K inhibitor LY294002 is designed as a novel photothermal agent for inhibitor and photothermal synergistic therapy of tumors under mild photothermal therapy conditions. The LY294002 of PI3K inhibitor, after being loaded by Bi 2 S 3 –Tween 20 nanodots, exhibits greatly increased drug utilization and reduced side effects on normal tissues. In vivo, Bi 2 S 3 –Tween 20@LY294002 upon near-infrared 808 nm laser irradiation shows potent antitumor activity under relatively mild conditions (power density: 0.6 W cm −2 ). Moreover, the mechanism studies also demonstrate that Bi 2 S 3 –Tween 20@LY294002 potently kills LoVo cancer cells under low-power near-infrared light irradiation, by downregulating the expression of heat shock protein 70 (HSP70) so as to increase the sensitivity of tumor cell hyperthermia and activating BAX/BAK-regulated mitochondrial apoptosis pathway. The results demonstrate that the newly synthesized multifunctional nanoplatform paves a new avenue for accurate therapy of photothermal-resistant cancer.
研究领域:
纳米技术
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癌症治疗
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药学科学
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生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Yinping Huang,Baolin Zhang,Songbo Xie,Boning Yang,Qin Xu,Jie Tan
The methods for the delivery of theranostic agents across the blood–brain barrier (BBB) are highly required. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with PEG (poly(ethylene glycol)), PEI (poly(ethylene imine)), and Tween 80 (polysorbate 80) (Tween-SPIONs) were prepared. We demonstrate the effective passage of tail-vein-injected Tween-SPIONs across normal BBB in rats under an external magnetic field (EMF). The quantitative analyses show significant accumulation of SPIONs in the cortex near the magnet, with progressively lower accumulation in brain tissues far from the magnet. A transmission electron microscopy picture of an ultrathin section of the rat brain displays Tween-SPIONs crossing the BBB. The comparative study confirms that both the Tween-80 modification and EMF play crucial roles in the effective passage of SPIONs across the intact BBB. However, the magnetic force alone cannot drag the SPIONs coated with PEI/PEG polymers through the BBB. The results indicate the Tween-SPIONs cross the BBB via an active penetration facilitated by EMF. This work is encouraging for further study on the delivery of drug or diagnostic agents into the parenchyma of the brain for dealing with neurological disorders by using Tween-SPIONs carriers under EMF.
研究领域:
纳米医学
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神经药理学
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药物递送系统
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生物医学工程
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磁性纳米颗粒
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Zhang Li,Jie Fu
The induced-formation of natural nanoparticles by photosensitive chemicals in aquatic environment may be a ubiquitous process, however, we still know very little on this issue. Here, we reported a potential original pathway for naturally-occurring gold nanoparticles (N-AuNPs) via the reduction of Au ions by photosensitive surfactant Tween 80/85 under light irradiation in the aquatic environment. The heterolytic reaction of hydroxyl groups in Tween 80/85 with the aid of UV light yielded radical anions (Tween 80/85-O •− ) and subsequently transferred electrons to oxygen to generate superoxide radicals (O 2 •− ), which reduced Au(III) to Au(0). The negatively charged Tween 80/85-O •− could bind to the surface of Au with coordinate bonds to form the surface coating of N-AuNPs. Due to the enhanced negative charge density, the Tween 80/85-induced N-AuNPs showed a higher colloidal stability than other N-AuNPs (induced by humic acid) or engineered AuNPs (E-AuNPs). The surface coating of Tween 80/85 might hinder the attachment and internalization of N-AuNPs into bacterial cells, and bacteria could also extend the utilization range of carbon sources substrates to mitigate the adverse effects of N-AuNPs. Therefore, Tween 80/85-induced N-AuNPs exhibited relative lower ecotoxicity but could stay in water for long periods of time. This study makes us more aware of the role of anthropogenic organic pollutants in the geochemical process of metal elements.
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DOI
高影响因子
Food Bioscience
·
2021
·
IF 4.24
Kehong Hou,Yaoyao Xu,Kaiyue Cen,Chengcheng Gao,Xiao Feng,Xiaozhi Tang
Essential oils (EOs) are gaining great interest as alternatives to synthetic food preservatives due to their antimicrobial activity . However, EOs are hydrophobic and easy to evaporate. In this research, EOs were encapsulated in nanoemulsions with the aid of hydroxypropyl-β-cyclodextrin (HPCD) to protect the ingredients and sustain the release, so as to exert long-term antibacterial effects. The influence of HPCD and its concentration on cinnamon essential oil (CEO) nanoemulsion formation, antibacterial activity , release property and stability was investigated. The results showed that HPCD reduced the particle size of nanoemulsion as the concentration of Tween 80 ranged from 2 to 4 g/L. However, HPCD addition showed adverse effect as the content of Tween 80 was 5 g/L. Antibacterial tests against Escherichia coli and Staphylococcus aureus demonstrated that the addition of HPCD contributed to the increase of antimicrobial activity of CEO nanoemulsions and showed the best effect when the concentration of HPCD was 30 g/L. The release experiment proved the slow-release ability of HPCD, and showed that the cavity of HPCD helped to reduce the loss of CEO, thus delayed the release time and enhanced the antibacterial effect. Meanwhile, the use of HPCD contributed to the storage stability of the emulsion at 4, 25, and 40 °C, and the nanoemulsion remained stable during a storage period of 60 days.
研究领域:
食品科学
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微生物学
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药物递送系统
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Xiaoxia Zhang,Ru Song,Xinyan Liu,Yan Xu,Rongbian Wei
Poor water solubility is one of the main adverse factors for vitamin D 3 to be used as a fortifier in water-soluble food system. In this study, a phase transformation based nanoemulsion fabrication process was developed to encapsulate vitamin D 3 with Tween 80 and Span 80 as a composite surface-active surfactant (named as Vit D 3 NEs). The encapsulation efficiency of vitamin D 3 in resultant oil in water nanoemulsions reached 99.9%. The prepared Vit D 3 NEs was spherical with average particle size of 391.47 ± 12.89 nm, polydispersity index of 0.23–0.31, and endothermic temperature of 127.1 ℃. Results of FTIR indicated that hydrophobic, electrostatic and hydrogen bond interactions were involved in Vit D 3 NEs fabrication. Compared to un-encapsulated Vit D 3 (bare Vit D 3 ), Vit D 3 NEs displayed higher stability under gastric digestion, and sustained release under intestinal digestion. The cumulative release of Vit D 3 reached 69% after 5 h of intestinal digestion. Further, Vit D 3 NEs demonstrated stronger thermal stability than the bare Vit D 3 at 40–60 °C, and the first-order kinetics was well fit to describe its thermal degradation . Our work suggests a new insight into the application of Vit D 3 NEs stabilized by Tween 80 and Span 80 in food industry.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Shenglan Liao,Gang Yang,Zhirong Wang,Yuheng Ou,Shan Huang,Bin Li,Aijun Li,Jianquan Kan
Staphylococcus epidermidis has been identified as a pathogen in ready-to-eat foods, and its biofilm can cause devastating chronic infections in affected hosts. For the demand of natural antibacterial reagents and overcoming their inherent properties, nanoemulsions are increasingly being used in the delivery of natural drugs. The nanoemulsion obtained by ultrasonic technology has the advantages of small droplet size , low polydispersity index (PDI), high stability and small amount of surfactant. Therefore, ultrasonic technology was used to prepare Zanthoxylum schinifolium Sieb. et Zucc essential oil nanoemulsion (ZEO-NE), a novel antibiofilm nanoemulsion with a mean particle size of 105.6 nm, polydispersity index of 0.278, and Zeta potential of − 22.3 mV. Assessment of antibacterial activity defined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration of ZEO-NE against S. epidermidis as 2.5 and 5.0 mg/mL, respectively. Meanwhile, the antibiofilm assays revealed that the inhibition rate of ZEO-NE at sub-MIC levels (1/16–1/2 MIC) against S. epidermidis biofilm formation was 4.65–81.38 %. Further studies showed that sub-MIC ZEO-NE can effectively inhibit the formation of biofilms by extracellular polymeric substances and regulate gene expression of biofilm initial adhesion, formation, and quorum-sensing systems. Meanwhile, the inhibition rates of ZEO-NE (1/2–4 MIC) on preformed biofilm biomass and cell metabolic activity were 45.06–72.40 % and 30.72–62.54 %, respectively. In addition, electron microscopy analysis showed that ZEO-NE can destroy the established biofilms to a great extent. Finally, cytotoxicity evaluation experiments showed that ZEO-NE could damage human immortalized keratinocytes . These findings indicate that nanotechnology is an effective strategy to increase ZEO stability and solubility, and the prepared ZEO-NE is a viable candidate to inhibit S. epidermidis and its biofilms.
研究领域:
纳米技术
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微生物学
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药学
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食品科学
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生物技术
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Zhibin Chen,Wenbo Wang,Jie Xiao
The interfacial-bulk phase distribution behavior of antioxidants is closely related to their anti-oxidative efficiency. In this study, the dynamic distribution behavior of gallic acid (GA) in a zein particles-Tween 20 co-stabilized emulsions (CPE) and its impact on the oxidative stability of CPE were elucidated via a combined experimental and Dissipative Particle Dynamics (DPD) method. As the concentration of TW20 increased, the competitive displacement of Zp from the interface was observed. Consequently, the interfacial loading of Zp decreased, leading to a corresponding decrease in the content of GA on the interface. The study's pseudo-phase model provided quantitative data indicating that the interfacial partition percentage of GA decreased from 70.72% to 48.63%, while the interfacial adsorption rate of GA decreased from 0.039 to 0.015. A notable discovery was made in emulsions solely stabilized by Zp, where 17.58% of GA was lost at the interface through free diffusion. However, in CPE, this loss was significantly reduced to 9.12%. This reduction was achieved by maintaining an appropriate concentration of TW20 (ranging from 0.19 to 1.91 mM), which facilitated a higher interfacial distribution of GA in CPE. Additionally, inhibiting the free diffusion of antioxidants at the interface, as indicated by the diffusion coefficients ranging from 20.51 to 4.45, contributed to the improved oxidative stability of the CPE. The study not only provided insights into the phase-interface distribution behavior of antioxidants in emulsions but also offered in-situ determination methods to guide the interfacial engineering of emulsions.
研究领域:
食品科学
·
化学工程
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产品关联
DOI
高影响因子
Micromachines
·
2023
·Vol. 14
·期 5
·902
·
IF 3.4
Yong Xie,Chao Bian,Mingjie Han,Ri Wang,Yang Li,Yuhao Xu,Shanhong Xia
A highly sensitive and accurate dual-signal strategy is developed for trace Cu2+detection based on the inner filter effect (IFE) between Tween 20-gold nanoparticles (AuNPs) and CdSe/ZnS quantum dots (QDs). Tween 20-AuNPs are utilized as colorimetric probes and excellent fluorescent absorbers. The fluorescence of CdSe/ZnS QDs can be quenched efficiently by Tween 20-AuNPs via IFE. In the presence of D-penicillamine, D-penicillamine induces the aggregation of Tween 20-AuNPs and the fluorescent recovery of CdSe/ZnS QDs at high ionic strength. Upon addition of Cu2+, D-penicillamine tends to selectively chelate with Cu2+and then forms the mixed-valence complexes, which consequently inhibits the aggregation of Tween 20-AuNPs and the fluorescent recovery. The dual-signal method is used to quantitatively detect trace Cu2+, with low detection limits of 0.57 μg/L and 0.36 μg/L for colorimetry and fluorescence, respectively. In addition, the proposed method using a portable spectrometer is applied to the detection of Cu2+in water. This sensitive, accurate and miniature sensing system has potential in environmental evaluations.Keywords:fluorescence;colorimetry;copper;CdSe/ZnS quantum dots;gold nanoparticles;inner filter effect
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
AMB Express
·
2021
·Vol. 11
·期 1
·1-13
·
IF 3.298
Zhuang Wenjin,Liu Hanyu,Zhang Ying,He Junyao,Wang Pu
( R )-1-[3-(Trifluoromethyl)phenyl]ethanol (( R )-MTF-PEL) is an important chiral building block for the synthesis of a neuroprotective compound, ( R )-3-(1-(3-(trifluoromethyl)phenyl)ethoxy)azetidine-1-carboxamide. In this work, an effective whole-cell-catalyzed biotransformation was developed to produce ( R )-MTF-PEL, and its productivity was increased by medium engineering strategy. The recombinant E. coli BL21(DE3)-pET28a(+) - LXCAR-S154Y variant affording carbonyl reductase was adopted for the reduction of 3'-(trifluoromethyl)acetophenone to ( R )-MTF-PEL with enantiomeric excess (ee) > 99.9%. The addition of 0.6% Tween-20 (w/v) boosted the bioreduction, because the substrate concentration was increased by 4.0-fold than that in the neat buffer solution. The biocatalytic efficiency was further enhanced by introducing choline chloride: lysine (ChCl:Lys, molar ratio of 1:1) in the reaction medium, because the product yield reached 91.5% under 200 mM substrate concentration in the established Tween-20/ChCl:Lys-containing system, which is the highest ever reported for ( R )-MTF-PEL production. The optimal reduction conditions were as follows: 4% (w/v) ChCl:Lys, 12.6 g (DCW)/L recombinant E. coli cells, pH 7.0, 30 ℃ and 200 rpm, reaction for 18 h. The combined strategy of surfactant and NADES has great potential in the biocatalytic process and the synthesis of chiral alcohols.
研究领域:
生物化学
·
合成生物学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2014
·
IF 4.48
Chun-Feng Guo,Jing-Yan Li
The effects of a Tween 80 and CaCl 2 mixture on the hypocholesterolemic activity of bile salt hydrolase (BSH)-active Lactobacillus casei F0422 were investigated in rats fed a cholesterol-rich diet for 3 weeks. This mixture significantly enhanced the hypocholesterolemic activity of strain F0422 in rats. The group administered drinking water supplemented with both strain F0422 and the Tween 80 and CaCl 2 mixture had significantly lower levels of total serum cholesterol, low-density lipoprotein cholesterol and total liver cholesterol than did the group administered drinking water supplemented only with strain F0422 or the Tween 80 and CaCl 2 mixture. The hypocholesterolemic activity of the combined strain F0422 and Tween 80 and CaCl 2 mixture was attributed to the increase in fecal bile acid excretion through enhancement of bile salt tolerance and BSH catalytic activity of strain F0422 by Tween 80 and up-regulation of the adhesion of strain F0422 to intestinal epithelial cells by CaCl 2 .
研究领域:
营养学
·
微生物学
·
药理学
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产品关联
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Chunyang Liu,Yanrong Cheng,Delan Ge,Ju Xie,Xia Guo
Aqueous two-phase systems (ATPS) are considered a new type of green separation system, but their low selectivity to target molecules limits their practical application. In this study, we used surfactant@MWCNT as an adjuvant to improve the selectivity of PEG/(NH4)2SO4 ATPS. The surfactants used include Triton X-100 (TX-100), Tween 80, sodium dodecyl sulfate (SDS), sodium dodecyl benzenesulfonate (SDBS), and bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Nine aromatic compounds, including syringic acid, vanillin, tryptophan, phenylalanine, tyrosine, mandelic acid, theophylline, theobromine, and cephalexin, were selected as model extracts. The results showed that the use of the surfactant@MWCNT composite as an adjuvant significantly improved the selectivity of the ATPS toward syringic acid among the nine extracts. Moreover, the selectivity of the ATPS for syringic acid with the TX-100/MWCNT composite as the adjuvant is better than that with the Tween 80/MWCNT composite as the adjuvant, followed by that with the anionic surfactant/MWCNT composite as the adjuvant. The enhanced selectivity of ATPS by the surfactant/MWCNT composite is ascribed to the spatial effect and the electrostatic and hydrophobic interactions between the extract and the surfactant adsorbed on the MWCNT.
研究领域:
化学工程
·
分离科学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）