ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Lin Yang,Ming Xuan Gao,Hong Yan Zou,Yuan Fang Li,Cheng Zhi Huang
Nature enzyme-based immunoassays have been widely used in fundamental scientific research and clinical diagnosis. However, the limitations of natural enzyme, such as the low physical/chemical stability or susceptibility to protein denaturation, greatly restrained its applications. In this article, we reported a new enzyme-free SERS immunoassay by utilizing plasmonic Cu2–xSySe1–y nanoparticles (NPs) as nanocatalyst to catalyze the click chemistry between the azido and alkynyl substrate which is used as the SERS signal reporter. The unique vibration of C≡C of alkynyl in the Raman-silent region (1800–2800 cm–1) is not overlapped with the signals of the other conventional Raman reporters or endogenous biological species, and thus it can make sure the enzyme-free SERS immunoassay has high selectivity and sensitivity. As a proof of concept, prostate-specific antigen (PSA), a biomarker of prostate cancer in blood, has been detected. The SERS immunoassay shows good analytical performance for PSA in the range of 3–120 ng mL–1, and it has been successfully applied to detect PSA in the serum samples of prostate cancer patients, proving that the proposed enzyme-free SERS immunoassay has great potential in the clinical diagnosis of cancer.
研究领域:
生物医学工程
·
癌症诊断
·
纳米技术
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 24
·5584-5592
·
IF 5.066
Xiaolong Li,Shirong Wang,Yin Xiao,Xianggao Li
Based on Y-type titanylphthalocyanine nanoparticles (Y-TiOPc NPs) with an average diameter of 2.5 ± 0.6 nm, a trap-assisted ultrasensitive near-infrared organic photodetector (NIR OPD) has been successfully fabricated using the dip-coating process. The prepared OPD exhibited an obvious photomultiplication phenomenon with the highest external quantum efficiency (EQE) of 354 200%, an excellent photosensitivity with the maximum photoresponsivity (R) of 2227 A W−1, and an outstanding low-light detection with the highest normalized detectivity (D*) of 3.1 × 1014 Jones under 780 nm illumination with a low intensity of 0.1 μW cm−2 at +15 V μm−1. Such performances were attributed to the ultrasmall Y-YiOPc NPs with remarkable photosensitivity and a high surface-to-volume ratio which can enhance external hole tunneling injection assisted by trapped-electrons at the photoactive layer/ITO interface. The high EQE and R properties of the device, as well as the prompt, steady and reproducible photocurrent response during repeated on/off cycles of illumination, suggested that the prepared OPD was desired for ultrasensitive NIR detection. Therefore, the ultrasmall Y-TiOPc NPs proposed in this study showed great promising application in the large-area and low-cost solution process of ultrasensitive NIR OPDs.
研究领域:
材料科学
·
光学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 43
·20157-20170
·
IF 3.3
Xiaojun Dai,Yan Cheng,Tingting Liu,Lingjuan Mao,Ruihua Kang,Qiang Wei,Yasong Zhou
In the process of petroleum refining, realizing the production and utilization of chemical raw materials is the common goal pursued by the petrochemical industry at present. The integration of the refining and chemical industry can not only produce clean fuel, but also meet the demand of the chemical industry for raw materials. In this study, Y zeolites with controllable crystal size and particle size were successfully prepared by simply adjusting the composition of the directing agent. Through a series of characterization, the effects of the composition change of the directing agent on the physicochemical properties of Y zeolites were investigated. Corresponding NiWS supported catalysts were prepared by using synthesized Y zeolites as supports. Taking naphthalene as the model reactant, the effects of crystal size and particle size of Y zeolites on the catalytic performance of the catalyst and on the hydrocracking of naphthalene to C6–C8 products were investigated. The results show that the preparation of small crystal Y zeolites can be realized by simply adjusting the composition of the directing agent, and the corresponding catalyst can realize the efficient production of chemical raw materials. This is expected to provide a simple, economical, feasible, and effective idea for the petrochemical industry to realize the goal of refining and chemical integration.
研究领域:
石油化学工程
·
催化材料科学
✓ 已验证
DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Fan Shao,Fengzhi Guo,Tianyu Bai,Zhen Xu,Han Yang,Wenbo Kang,Yasong Zhou,Wenbin Huang,Gang Wang,Qiang Wei
The inherent diffusion constraints imposed by narrow micropores and elongated channels in Y zeolites severely limit jet fuel selectivity during Fischer-Tropsch wax hydrocracking. Advanced supports were synthesized via seed-directed crystallization (nano-Y-0P zeolite) and PDDA (polydiallydimethyl ammonium chloride)-mediated assembly (hierarchical nano-Y-xP zeolites, x = 0.1, 0.3, 0.6, 0.9). This dual-strategy synergistically addresses diffusion constraints in conventional Y-zeolite by optimizing nano structure of active sites and improving interfacial mass transfer (i.e., nano-crystal size and hierarchical porosity). Intelligent Gravimetric Analysis (IGA) directly validates hierarchical porosity and nanocrystalline advantages. Y-0.6P zeolite with optimal mesoporosity (0.75 cm 3 /g vs. 0.25 cm 3 /g in conventional Y-zeolite) and minimal crystallite size (131 nm vs. 1258 nm in conventional Y-zeolite) achieves intrinsic diffusion enhancement—the 5.36-fold higher diffusion rate than conventional S-Y zeolite. Zeolite crystallite size and hierarchical porosity critically govern metal–acid functionality balance by modulating acid site density (589.7 μmol/g vs. 812.4 μmol/g in conventional Y zeolite) and optimizing metal dispersion (40.5 %, vs. 18.26 % in conventional Y-zeolite). Pt/(Y-0.6P+ASA) catalyst achieves peak performance with 35.04 % C 9 –C 17 yield and 47.35 % selectivity in n-docosane hydrocracking. Notably, Fischer-Tropsch wax hydrocracking yields 26.83 % jet fuel (49.88 % selectivity) with ideal bell-shaped distribution. This work demonstrates Y-0.6P-supported catalyst has industrial potential for aviation fuel production from Fischer-Tropsch wax upgrading.
研究领域:
催化化学
·
能源化工
·
材料科学
·
石油炼制
·
绿色能源
✓ 已验证
DOI
高影响因子
CORROSION SCIENCE
·
2024
·
IF 7.4
Keda Xiao,Yi Zeng,Xiang Xiong,Jinrun Hu,Shiyan Chen,Tianyou Li
Si-B-Mo-Y modified SiC/SiC composites were prepared by reactive melt infiltration (RMI) process to study their high-temperature water-oxygen corrosion resistance, and to compare them with SiC/SiC composites prepared by RMI using Si. The results show that the corrosion rates of the modified composites are only 1/9, 1/10 and 1/3 of those of the comparison samples at 1200°C, 1300°C and 1400°C, respectively, which is attributed to the higher densification of the modified composites. More importantly, the modified composites were able to form borosilicates, which effectively healed the oxide film defects; meanwhile, yttrium silicate inhibited the reaction between borosilicates and water vapor.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Huanshun Yin,Xinxu Wang,Yunlong Guo,Yunlei Zhou,Shiyun Ai
An effective assay method for monitoring protein kinase activity and screening inhibitors is greatly beneficial to kinase-related drug discovery, early diagnosis of diseases, and therapeutic effect evaluation. Herein, we develop a simple electrochemical method for detecting the activity of casein kinase II (CK2) based on phosphorylation against carboxypeptidase Y (CPY) digestion triggered signal amplification , where CK2 catalyzed phosphorylation event protects the substrate peptide from the digestion of CPY, maintains the repulsive force of the substrate peptide towards the redox probe, and results in a weak electrochemical signal. Whereas, without phosphorylation, the substrate peptide is digested by CPY and a strong electrochemical signal is obtained. The detection feasibility is demonstrated for the assay of CK2 activity with low detection limit of 0.047 unit/mL. Moreover, the biosensor was used for the analysis of kinase inhibition. Based on the electrochemical signal dependent inhibitor concentration, the IC 50 value of ellagic acid was estimated to be 39.77 nM. The proposed method is also successfully applied to analyze CK2 activity in cell lysates, proving the applicability in complex biological samples.
研究领域:
生物化学
·
分析化学
·
药物发现
·
疾病诊断
✓ 已验证
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Pusheng Liu,Ying Cui,Jianyu Wang,Xiaohui Du,Haitao Zhang,Adrian Humphries,Mingjun Jia,Jihong Yu
Rare earth (RE)-exchanged zeolite Y is the major active component of fluid catalytic cracking (FCC) catalysts. Herein, a series of yttrium (Y 3+ )-exchanged zeolites Y was prepared by the ion-exchange method, and the location of Y 3+ ions in the Y 3+ -exchange zeolite NaY was determined by fitting synchrotron radiation X-ray powder diffraction (XRPD) pattern combined with the infrared spectra in the hydroxyl stretching region. In analogy to lanthanum (La 3+ )-exchanged zeolite Y, Y 3+ ions were preferentially located inside the sodalite units near the double six-membered rings (D6R's), octahedrally coordinating to three framework oxygens and to three H 2 O molecules (or OH groups) in close proximity. The substitution of Y 3+ ions for counter-ions Na + caused a noticeable shrinkage of the unit cell due mainly to the relatively small ionic radius and high charge density of Y 3+ ions. As a consequence, the Y 3+ -exchanged HY-based FCC catalyst exhibited improved steam stability, and higher selectivity for high-value products (liquefied petroleum gas and C 5 +gasoline) in n-docosane cracking compared with the La 3+ -exchanged HY-based catalyst.
研究领域:
化学工程
·
催化材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jiaojiao Zhou,Wenjing Wang,Shuojun Li,Axiu Nie,Zhiyong Song,Mohamed F. Foda,Zhicheng Lu,Ling Zhao,Heyou Han
An innovative fluorescent and colorimetric dual-mode strategy based on Y-shaped DNA was proposed for the detection of rabies virus oligonucleotide. The cascade assembly of hairpin probes was initiated by the target, leading to the formation of Y-shaped DNA accompanied by freeing target to catalyze the next assembly process. The fluorescence recovery of the fluorophore FAM was accompanied by opening of hairpin probe. A colorimetric signal was generated by the proximity of glucose oxidase (GOx) and horseradish peroxidase (HRP), which was reflected by enhanced catalytic capability to TMB. Using this method, target DNA can be detected with a wide linear range from 0.1 to 6 nM with a detection limit of 15 pM, and from 0.5 to 60 nM with a detection limit of 60 pM for fluorescent and colorimetric readout, respectively. Moreover, the dual-mode detection strategy exhibited excellent selectivity towards the target compared with interference oligonucleotides. In addition, recoveries ranging from 96.0% to 106% with RSD values varying from 3.2% to 5.4%, and from 91.0% to 102% with RSD values varying from 3.3% to 7.1% for fluorescent and colorimetric studies, respectively, were obtained in diluted cerebrospinal fluid samples. The proposed dual-mode sensor is easy to operate with excellent detection performance. It would be superior to single mode detection and more practicable for quantitative detection of nucleic acid-related biomarkers due to the self-correction capability.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
病毒学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Peizong Deng,Fangfang Teng,Feilong Zhou,Zhimei Song,Ning Meng,Na Liu,Runliang Feng
Ketoconazole is a hydrophobic broad-spectrum antifungal agent for skin infection therapy. In order to develop topical formulation of ketoconazole for improving its selective skin deposition and water-solubility, ketoconazole-loaded Y-shaped monomethoxy poly(ethylene glycol)-block-poly(ɛ-caprolactone) micelles were prepared through thin-film hydration method with high entrapment efficiency (96.1 ± 0.76%) and small particle (about 58.66 nm). The drug-loaded micelles showed comparative in vitro antimicrobial activity with KET cream. In e x in vivo skin deposition and permeation study, ketoconazole-loaded micelles provided skin accumulation higher than marketed ketoconazole cream without obvious permeation in the whole period. Fluorescence microscopy study and histopathological study demonstrated the copolymeric micelles' penetrating into skin in depth due to its capability of weakening the barrier function of stratum corneum. In vivo skin deposition parameters further confirmed high skin deposition of drug-loaded micelles (AUC (0-t) = 396.16 μg·h/cm 2 ) over marketed ketoconazole cream (AUC (0-t) = 250.03 μg·h/cm 2 ). Meanwhile, in vivo pharmacokinetic parameters proved that ketoconazole-loaded micelles reduced ketoconazole's distribution in blood in comparison with the cream (AUC (0-t) = 93,028.00 μg·h/L vs AUC (0-t) = 151,714.00 μg·h/L), meaning lower possibility of its systemic unwanted effects in the skin fungal infection treatment. The results suggested that the copolymeric micelles can be adopted for specific delivering ketoconazole into skin for fungal infection cure.
研究领域:
药剂学
·
皮肤病学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
SYNLETT
·
2023
·
IF 2
Shengyu Yu,Jiahao Liu,Zhe Wang,Terumasa Kato,Yan Liu,Keiji Maruoka
A series of soluble Y-shaped trimers of chiral phase-transfer catalysts were synthesized and utilized in the asymmetric alkylation of amino acid Schiff bases. The length of the linker between the ammonium cation of the simplified Maruoka catalyst and a phloroglucinol moiety was varied and excellent yields and enantioselectivity were observed when using the Y-shaped trimer of chiral phase-transfer catalyst with an octamethylene linker. A theoretical study of the most efficient Y-shaped trimer of chiral phase-transfer catalyst suggests the chiral ammonium center of the catalyst is more exposed and thus more available in the reaction.
研究领域:
有机化学
·
催化化学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 2
·705-713
·
IF 3.288
Samuthirarajan Senthilkumar,Wei Zhong,Mookan Natarajan,Chunxin Lu,Binyu Xu,Xiaoming Liu
An efficient and green protocol for aerobic oxidation of benzylic alcohols in ethanol using CuI–Y zeolite catalysts assisted by TEMPO (TEMPO = 2,2,6,6-tetramethyl-1-piperidine-N-oxyl) as the radical co-catalyst in the presence of atmospheric air under mild conditions is reported. The CuI–Y zeolite prepared via ion exchange between CuCl and HY zeolite was fully characterized by a variety of spectroscopic techniques including XRD, XPS, SEM, EDX and HRTEM. The incorporation of Cu(I) into the 3D-framework of the zeolite rendered the catalyst with good durability. The results of repetitive runs revealed that in the first three runs, there was hardly a decline in activity and a more substantial decrease in yield was observed afterwards, while the selectivity remained almost unchanged. The loss in activity was attributed to both the formation of CuO and the bleaching of copper into the liquid phase during the catalysis, of which the formation of CuO was believed to be the major contributor since the bleaching loss for each run was negligible (<2%). In this catalytic system, except TEMPO, no other additives were needed, either a base or a ligand, which was essential in some reported catalytic systems for the oxidation of alcohols. The aerobic oxidation proceeded under mild conditions (60 °C, and 18 hours) to quantitatively and selectively convert a wide range of benzylic alcohols to corresponding aldehydes, which shows great potential in developing green and environmentally benign catalysts for aerobic oxidation of alcohols. The system demonstrated excellent tolerance against electron-withdrawing groups on the phenyl ring of the alcohols and showed sensitivity to steric hindrance of the substrates, which is due to the confinement of the pores of the zeolite in which the oxidation occurred. Based on the mechanism reported in the literature for homogenous oxidation, a mechanism was analogously proposed for the aerobic oxidation of benzylic alcohols catalysed by this Cu(I)-containing zeolite catalyst.
研究领域:
化学
·
催化
·
绿色化学
·
材料科学
✓ 已验证
DOI
高影响因子
BIOMATERIALS
·
2016
·
IF 8.387
Juan Li,Yuchen Tian,Dingying Shan,An Gong,Leyong Zeng,Wenzhi Ren,Lingchao Xiang,Ethan Gerhard,Jinshun Zhao,Jian Yang,Aiguo Wu
Targeted molecular imaging has attracted great attention in cancer diagnosis and treatment. However, most clinically used ultrasound contrast agents (UCAs) are non-targeted microbubbles seldom used for cancer imaging. Here, we fabricated fluorescent nanobubbles (NBs) by encapsulation of liquid tetradecafluorohexane (C 6 F 14 ) within biodegradable photoluminescent polymers (BPLPs) through an emulsion-evaporation process and conjugation of PNBL-NPY ligand for specific targeting of Y 1 receptors overexpressed in breast tumors. The developed PNBL-NPY modified NBs were uniform in size with good dispersibility and photostability, presenting good ultrasound enhancement. Further, in vitro and in vivo results indicated that the fabricated NBs exhibit high affinity and specificity to Y 1 receptor-overexpressing breast cancer cells and tumors with minimal toxicity and damage to organs. Our developed PNBL-NPY-modified NBs are novel targeted UCAs for safe, efficient and specific targeted breast cancer imaging, and may provide a new nanoplatform for early cancer diagnosis and treatment in the future.
研究领域:
医学影像
·
癌症诊断
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）