TALANTA
·
2021
·
IF 6.057
Yuan-Yuan Jiang,Xu Zhao,Li-Jian Chen,Cheng Yang,Xue-Bo Yin,Xiu-Ping Yan
Mycotoxins contamination in agricultural products poses a serious threat to human and animal health, so rapid and sensitive nanosensors for simultaneous determination of multiple mycotoxins in food samples are highly desirable for food safety monitoring. Herein, we report the fabrication of functional dual-colored persistent luminescence nanoparticles (PLNPs) in conjunction with Fe 3 O 4 magnetic nanoparticles as a nanosensor for the simultaneous biosensing of aflatoxin B 1 (AFB 1 ) and zearalenone (ZEN) in food samples. Two types of PLNPs with a single excitation wavelength, Zn 2 GeO 4 :Mn 2+ and Zn 1.25 Ga 1.5 Ge 0.25 O 4 :Cr 3+ ,Yb 3+ ,Er 3+ , are employed as the signal units, and aptamers with high affinity and specificity to the corresponding mycotoxins are used as the recognition units. The nanosensor was fabricated by hybridizing the aptamer modified PLNPs with the complementary DNA modified Fe 3 O 4 . The developed nanosensor offers the integrated merits of autofluorescence-free detection of persistent luminescence, the high specificity of aptamer and the high speed of magnetic separation, allowing highly sensitive and selective detection of AFB 1 and ZEN in food samples with the limits of detection of 0.29 pg mL −1 for AFB 1 and 0.22 pg mL −1 for ZEN and the recoveries of 93.6%–103.2% for AFB 1 and 94.7%–105.1% for ZEN. This work also provides a novel universal PLNPs-based optical platform for the simultaneous detection of multiple contaminants in complex samples.
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高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Shaolan Xu,Ganggang Zhang,Bolong Fang,Qirong Xiong,Hongwei Duan,Weihua Lai
In this work, polydopamine-coated gold nanoparticles (Au@PDAs) were synthesized by the oxidative self-polymerization of dopamine (DA) on the surface of AuNPs and applied for the first time as a signal-amplification label in lateral flow immunoassays (LFIAs) for the sensitive detection of zearalenone (ZEN) in maize. The PDA layer functioned as a linker between AuNPs and anti-ZEN monoclonal antibody (mAb) to form a probe (Au@PDA-mAb). Compared with AuNPs, Au@PDA had excellent color intensity, colloidal stability, and mAb coupling efficiency. The limit of detection of the Au@PDA-based LFIA (Au@PDA-LFIA) was 7.4 pg/mL, which was 10-fold lower than that of the traditional AuNP-based LFIA (AuNP-LFIA) (76.1 pg/mL). The recoveries of Au@PDA-LFIA were 93.80–111.82%, with the coefficient of variation of 1.08–9.04%. In addition, the reliability of Au@PDA-LFIA was further confirmed by the high-performance liquid chromatography method. Overall, our study showed that PDA coating can chemically modify the surface of AuNPs through a simple method and can thus significantly improve the detection sensitivity of LFIA.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Binbin Zhou,Hao Xie,Sisi Zhou,Xingxin Sheng,Liang Chen,Ming Zhong
In this work, a highly sensitive and selective molecularly imprinted electrochemical sensor is exploited to detect zearalenone (ZEA) by the synergistic effect of reduced graphene nanoribbons (rGNRs) and gold nanoparticles (AuNPs). The oxidized GNRs are firstly produced by an improved Hummers’ oxidation method, and then reduced and modified together with AuNPs onto a glassy carbon electrode by electrodeposition technique to realize collaborative amplification of electrochemical signal. The molecularly imprinted polymer film with specific recognition sites can be generated on the modified electrode by electropolymerization. The effect of experimental conditions is systematically investigated to obtain the best detection performance. It is found that the constructed sensor shows a wide linear range of 1–500 ng·mL −1 for ZEA with a detection limit as low as 0.34 ng·mL −1 . Obviously, our constructed molecularly imprinted electrochemical sensor shows great potential in the application of precisely detecting ZEA in food.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yu-Fan Yang,Xiao-Peng Hu,Qing-Qing Luo,Song Liao,Hui-Wen Gu,Xiu-Fan Yan,Xiao-Li Yin
Zearalenone (ZEN) poses a threat by infiltrating the food chain and triggering various diseases; hence, the precise detection of ZEN is essential for ensuring food safety. Herein, a fluorescence sensor was constructed for sensitive and selective detection of ZEN based on magnetic molecular imprinted polymers (MIPs/Fe 3 O 4 @SiO 2 ) and carbon dots (CDs). MIPs/Fe 3 O 4 @SiO 2 , serving as recognition elements, captured ZEN in real samples. Using a magnet, we separated the MIPs/Fe 3 O 4 @SiO 2 that had captured ZEN and added them to a specific volume of the CDs solution, resulting in fluorescence quenching of the CDs. As the CDs were not directly exposed to the sample solution, these fluorescence probes exhibit a satisfactory level of anti-interference performance. Under optimal experimental conditions, an increase in ZEN concentration results in a linear decrease in the fluorescence intensity of the CDs. The sensor has a linear response range of 0.5–30 ng mL −1 with the limit of detection as low as 0.14 ng mL −1 (LOD = 3 σ/s). Furthermore, this developed sensor demonstrated high selectivity, sensitivity, and stability. This sensor has good application potential in the analysis of ZEN in complicated samples.
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ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Hongli Zheng,Huan Yi,Wen Lin,Hong Dai,Zhensheng Hong,Yanyu Lin,Xiuhua Li
Herein, a new sandwich-type electrochemiluminescence (ECL) immunosensor was established on the dual-roles of tetragonal rutile TiO 2 mesocrystals (TRM) to realize the sensitive detection of zearalenone (ZEA). On the one hand, the poly (amidoamine) dendrimers (PAAD)-functionalized TRM, combining the advantages of hyper-branched of PAAD and the high nanoporosity , large surface area of TRM, was served as the sensing matrix to immobilize enough capturing antibody, realizing the signal amplification. On the other hand, TRM was applied as an effective probe platform to absorb Ru (bpy) 3 2+ and simultaneously immobilize the secondary antibody (Ab 2 ). Here, based on the superior properties of TRM and the new bridge of 4-Mercaptophenylboronic acid (4-MPBA) for the high affinity of Ab 2 and TRM, a strong and stable ECL emission was realized. Additionally, cysteamine first emerging as a blocker was proved to sensitize the ECL response of Ru (bpy) 3 2+ , which further amplified the ECL signal, avoiding addition of exogenous coreactants into testing solution. Finally, the sandwich-type ECL immunosensor enabled the real samples detection of ZEA with a lower detection limit and wider linear range.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Qi Sun,Miaomiao Ma,Zhijuan Li,Xiang Chen,Jian Zeng,Meiqi Huang,Yuting Yin,Xueyan Ran,Jianmei Li,Tao Le
Zearalenone (ZEN) poses significant threats to food safety and human health due to its widespread contamination in cereals. Current detection methods face limitations in cost, complexity, and field applicability. To address this, we developed a novel colorimetric aptasensor leveraging the peroxidase-like activity of Au/CoOOH nanozymes for rapid and sensitive ZEN detection. Systematic characterization of cobalt-based nanomaterials (β-Co(OH) 2 , Co 3 O 4 , CoOOH, and Au/CoOOH) revealed that Au/CoOOH exhibited the highest catalytic efficiency, achieving a 3.5-fold enhancement over CoOOH due to synergistic interactions with Au nanoparticles. The aptasensor employed a competitive displacement strategy: ZEN binding to aptamer-functionalized magnetic beads released cDNA-conjugated Au/CoOOH, reducing 3,3′,5,5′-tetramethylbenzidine oxidation signals. Optimized parameters enabled a linear detection range of 0.3–500 ng/mL with a detection limit of 0.23 ng/mL. Specificity tests confirmed minimal cross-reactivity with other mycotoxins. The sensor demonstrated exceptional sensitivity, specificity, and practicality, validated by recovery rates of 90.65–105.63 % in spiked matrices, positioning it as a reliable tool for safeguarding food safety through rapid, precise mycotoxin monitoring.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Hongxia Tan,Hongyuan Zhou,Ting Guo,Yuhao Zhang,Liang Ma
Hidden mycotoxins have been reported to be “protected” by macromolecular substances to escape routine determination, but release to free mycotoxins under gastrointestinal conditions. Nowadays, the hidden zearalenone (ZEN) that binding with macromolecular zein has been found in maize. However, the binding mechanism of ZEN with zein in maize has not been clarified. In this study, the formation of ZEN-zein complex was investigated applying ultrafiltration, multi-spectroscopic and molecular modeling techniques. The steady-state and transient fluorescence analysis suggested the ZEN could interact with zein to form the complex driven by hydrophobic force and hydrogen bonds, which is in accordance with the molecular modeling studies. The conformational changes of zein induced by binding with ZEN were revealed by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD). Elucidating the binding mechanism between zein and ZEN could help the development of detecting hidden ZEN and guarantee the safety of maize products.
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Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 44
·6972-6980
·
IF 5.047
Yanjie Chen,Shupei Zhang,Zhensheng Hong,Yanyu Lin,Hong Dai
In this study, a mimotope peptide-based non-toxic photoelectrochemical (PEC) competitive enzyme-linked immunoassay (ELISA) was established for ultrasensitive detection of zearalenone (ZEN) with dual-signal readout. Using the phage display technique (PDT), a mimotope peptide of ZEN could be harvested by selecting a peptide from a phage-display peptide library, which avoided using mycotoxin itself and minimized potential damage to operators. The tyramine-modified rutile TiO2 mesocrystals (Tyr-RMC) with outstanding PEC properties were utilized as reporter units to label the mimotope peptide. The fabricated peptide@Tyr-RMC probe could anchor on the antibody-modified electrode via competitive immune recognition of free target ZEN. When subjected to catalysis of HRP–H2O2, the Tyr-RMC composite was deposited at the enzyme reaction site, causing rolling circle extension of the reporter unit chains. By merit of the brilliant signal amplification effect of tyramine signal amplification (TSA), dramatically enhanced photocurrent response and enormously increased electrochemical impedance could be determined. Combining all of these advantages, the developed dual-signal readout non-toxicity immunoassay could effectively decrease environmental interference. Also, the designed dual-signal readout biosensor demonstrated a wide linear range between 10−6 and 1 ng mL−1 with a low detection limit of 1 × 10−6 ng mL−1, which provides a valuable reference for developing highly efficient, secure and sensitive detection methods and indicates promising applicability in food testing.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Yongpeng Jin,Qian Chen,Sunlin Luo,Lidong He,Ruiqi Fan,Siwei Zhang,Chunjiang Yang,Yiqiang Chen
A novel dual near-infrared fluorescence-based lateral flow immunosensor was developed to determine zearalenone and deoxynivalenol in maize. Two near-infrared dyes with distinct fluorescence characteristics were utilized to separately label the anti-zearalenone and anti-deoxynivalenol antibodies as detection reagents. The capture antigens zearalenone-BSA and deoxynivalenol-BSA were mixed and immobilized on the same test line of nitrocellulose membrane. This assay format facilitates simultaneous detection of the two mycotoxins on a single test line. After optimizing experimental parameters, the limits of detection for zearalenone and deoxynivalenol were as low as 0.55 μg/kg and 3.8 μg/kg in maize, respectively. The spiking experiment yielded recovery ratios ranging from 81.7% to 107.3% with coefficients of variation less than 14% demonstrating high assay accuracy and precision. Moreover, the actual sample analysis produced consistent results between this method and instrumental method. Therefore, the developed immunosensor can serve as an accurate and efficient approach for monitoring mycotoxins in agricultural products.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·584
·
IF 5.4
Zheng Xiaoqi,Li Yuan,Long Jie,Zhang Tianyu,Zhou Binbin,Li Shan,Zhang Li
A composite modified electrochemical sensor was developed based on molecular imprinting technology for zearalenone (ZLE) detection. The sensor was fabricated on a glassy carbon electrode (GCE) by first electrochemically reducing and depositing graphene oxide nanoribbons (rGNR) to form a highly conductive three-dimensional network. Subsequently, palladium-metallene (Pd-Me) was coated to enhance the electrochemical response. Finally, acrylamide molecularly imprinted polymer (AMIP) was polymerized in situ under infrared light to impart specific recognition capability. Performance tests demonstrated that the fabricated sensor (AMIP/Pd-Me/rGNR/GCE) exhibited a linear response to ZLE in the range of 1.0 − 1000 ng/mL, with a detection limit of 0.33 ng/mL (S/ N = 3). The sensor also showed good selectivity, repeatability (signal attenuation ≤ 5.63% after five consecutive detections), reproducibility (relative standard deviation, RSD = 2.62%), and storage stability (96.85% signal retention after two weeks at 4 °C). When applied to actual samples (grain flour, soybean flour, and corn flour), the spiked recoveries ranged from 97.26% to 104.09%, with RSD below 4.58%. These results confirm that the sensor can achieve precise quantitative detection of ZLE in complex food matrices. The sensor preparation process, which combines electro-polymerization and coating, is characterized by simple operation, strong film formation, low cost, and ease of miniaturization, offering a promising new approach for the rapid determination of mycotoxins in food safety. Graphical
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Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 48
·10966-10976
·
IF 5.344
Han Fu,Junping Liu,Wu Xu,Haixiang Wang,Shenghua Liao,Guitang Chen
In this paper, a new magnetic molecular imprinted polymer–cyclodextrin (MMIP–CD) material was prepared by connecting β-cyclodextrin (CD) on the surface of a magnetic molecular imprinted polymer (MMIP) and used for the rapid and specific adsorption of zearalenone (ZEN). By using warfarin as the virtual template molecule, tetraethyl orthosilicate (TEOS) as the crosslinking agent, and (3-aminopropyl) triethoxysilane (APTES) as the functional monomer, a MMIP was produced by surface imprinting technology. Sulfobutyl ether-β-cyclodextrin attached to the surface of the MMIP under heating conditions produced a new specific adsorption material with exceptional adsorption capacity and excellent selectivity for ZEN. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and TEM-mapping results showed that the prepared MMIP–CD had a uniform particle size of about 480 nm, and the molecularly imprinted layer was successfully wrapped on the surface of the nanoparticles with a thickness of about 50 nm, whereby the cyclodextrin was effectively attached to the surface of the MMIP. The adsorption mechanism of MMIP–CD was confirmed by kinetic adsorption and thermodynamic adsorption experiments, the maximum adsorption capacity was found to be about 30 mg g−1, and the adsorption equilibrium could be reached within 20 min. The value of IF (QMMIP–CD/QMNIP) is 4.642. This showed that compared with MNIP, MMIP–CD showed a greatly improved specific adsorption capacity of ZEN. Selective experiments proved that MMIP–CD effectively combined the advantages of MMIP and CD, enhancing the adsorption capacity together with reducing the disadvantages that MMIP cannot distinguish structural analogs and CD cannot identify hydrophobic compounds effectively. In actual sample testing, the limit of quantification (LOQ) and limit of detection (LOD) were 0.1 ng kg−1 and 0.3 ng kg−1, respectively. The stability and detection precision of this method were 0.98–2.76% and 1.67–3.88%, respectively. The results proved that MMIP–CD had good development potential in the field of selective adsorption of ZEN, and laid the foundation for follow-up research.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 23
·5627-5635
·
IF 3.637
Tan Hongxia,Guo Ting,Zhou Hongyuan,Dai Hongjie,Yu Yong,Zhu Hankun,Wang Hongxia,Fu Yu,Zhang Yuhao,Ma Liang
Zearalenone (ZEN) is a type of estrogenic mycotoxin commonly occurring in cereals. The aim of this study was to design a simple, rapid, inexpensive and ultrasensitive fluorescence assay for the determination of ZEN. Here, amino-modified mesoporous silica nanoparticles (MSNs-NH 2 ) were synthesized to be the positive charge-rich reactor. A 6-carboxy-fluorescein-labeled aptamer (aptamer-FAM) was designed as the signal probe, ZEN-capture probe and negative charge reactor. In the absence of ZEN, the negatively charged aptamer-FAM combined with the positively charged MSNs-NH 2 in an electrostatic manner. In the presence of ZEN, the fluorescence intensity in the supernatant increased significantly because the aptamer-FAM could bind to ZEN instead of MSNs-NH 2 . Under the optimal experimental conditions, this assay exhibited excellent specificity, repeatability and a wide linearity range of 0.005–150 ng/mL, with a detection limit of 0.012 ng/mL. Additionally, it showed high recovery (83.3–101.5%) for the spiked samples. There was no statistically significant difference in the ZEN concentrations detected by the proposed assay and HPLC in naturally contaminated samples. Overall, this design provides a new strategy for the rapid, inexpensive and sensitive detection of ZEN, and it could be applied to develop fluorometric assays for different targets by the selection of appropriate aptamers.
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危险品化学品经营许可证（带存储）