Frontiers in Pharmacology
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2024
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IF 4.4
Chencen Lai,Shuo Zhang,Zhiyu Chen,Xiaoyu Fu,Min Zhang,Pengjiao Wang,Xiuli Gao
Pyroptosis induced by oxidative stress is a significant contributor to mental health disorders, including depression (+)-Catechin (CA), a polyphenolic compound prevalent in various food sources, has been substantiated by prior research to exhibit potent antioxidant properties and potential antidepressant effects. Nonetheless, the precise antidepressive mechanisms and effects of CA remain incompletely elucidated. In this study, we employed corticosterone (CORT) and PC12 cells to develop a cellular model of depression, aiming to investigate the protective effects of CA against CORT-induced cellular damage. Our objective was to elucidate the underlying mechanisms of protective action. We utilized transcriptomic analysis to identify differentially expressed genes and employed bioinformatics approaches to predict the potential mechanisms of CA’s protective effects in PC12 cells. These transcriptomic predictions were subsequently validated through western blot analysis. The findings indicated that CA possesses the capacity to mitigate oxidative stress and suppress pyroptosis in PC12 cells via the activation of the PI3K/AKT signaling pathway. This activation subsequently modulates the Nrf2/HO1/NF-κB pathways, thereby providing protection to PC12 cells against damage induced by CORT. Furthermore, we investigated the interaction between CA and the Keap1 protein employing molecular docking and protein thermal shift assays. We propose that CA can activate Nrf2 through two mechanisms to decrease reactive oxygen species (ROS) levels and inhibit pyroptosis: one mechanism involves the activation of the PI3K/AKT signaling pathway, and the other involves direct binding to Keap1, leading to an increase in p-Nrf2.
研究领域:
神经科学
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药理学
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细胞生物学
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分子生物学
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生物信息学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
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IF 10.4
Jin Zhao,JiaNing Xu,ShiQi Zhao,Chuang Ma,MengLi Li,Bin Li,Hui Min
The High-polymeric proanthocyanidins (PPC) derived from Aronia melanocarpa (AM) are prone to inducing digestive discomfort, restricting the development of related products. Meanwhile, traditional nucleophilic depolymerization requires high-cost monomers, underscoring the urgent need for improved methods. Herein, we employed response surface methodology optimizing the process of ultrasonic waves, a pulsed electric field (PEF), and electron beam irradiation for catechin nucleophilic depolymerization, further screening optimal depolymerization conditions for PPC. The structures of the depolymerization products were characterized by spectroscopy, chromatography, mass spectrometry, and thermodynamic analysis. The antioxidant activity was evaluated using the ABTS, DPPH, and hydroxyl radical scavenging assays, as well as mitochondrial membrane potential assays. Under optimal conditions, oligomers with lower average degrees of polymerization (DP) were produced: ultrasound+catechin (DP = 2.39 ± 0.15), PEF + catechin (DP = 2.20 ± 0.17), and electron beam+catechin (DP = 1.94 ± 0.15). For antioxidant activity, ABTS scavenging and cell protection followed the order: irradiation of electron beam combined with nucleophilic depolymerization of proanthocyanidin products (INPC) > electric field combined with nucleophilic depolymerization of proanthocyanidin products (ENPC) > ultrasound combined with nucleophilic depolymerization of proanthocyanidin products (UNPC) > nucleophilic depolymerization products (NPC) > PPC. Vitamin C (Vc) outperformed others in hydroxyl radical scavenging. All products were non-toxic to Caco-2 cells. This study indicates that the physical field-nucleophilic depolymerization system can effectively reduce the DP of PPC and enhance its antioxidant activity at a low catechin dosage, providing a new strategy for the economical and efficient depolymerization of PPC and boosting the development of the deep-processing industry for AM.
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FOOD CHEMISTRY
·
2018
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IF 4.946
Ruifang Bai,Yan Cui,Lanxin Luo,Danbing Yuan,Zongmin Wei,Weichao Yu,Baoshan Sun
Polymeric procyanidins (PPCs) were the major constituents of procyanidins, while they have poor bioactivity. To better utilize PPCs, a semisynthetic approach for converting PPCs to oligomeric procyanidins (OPCs) was proposed. Grape seed PPCs were simultaneously reacted with catechin (C) and epicatechin (EC) under acid condition. Combining response surface methodology (RSM) and single-factor experiments, an optimized semisynthetic condition was confirmed with the ratio of PPCs with C and EC of 1:1:1, temperature of 40 °C, reaction time of 20 min and 0.1 M methanolic HCl. High-speed counter-current chromatography (HSCCC) was adopted to obtain three fractions from semisynthetic products and preparative-HPLC was used to isolate individual procyanidins. Thirteen B-type procyanidins including monomers, dimers and trimers were got with high yield of 0.8–17.8 mg from 200 mg semisynthetic products and high purity over 91%. The developed semisynthesis combined with separation method was efficient to obtain individual OPCs in preparative scale.
研究领域:
植物化学
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天然产物化学
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药物化学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Ruonan Feng,Lulu Chen,Wanying Li,Tianming Cai,Canlan Jiang
In this study, we investigated the effects of different natural organic acids (NOAs), L -ascorbic acid (AA) and (+)-catechin hydrate (CAT), on the activation of persulfate (PDS) for the oxidation of naproxen (NAP) in water and soil. We found that only AA-activated PDS process had a significant degradation efficiency of NAP in water. High AA concentration (500 μM) inhibited the degradation of NAP, whereas high levels of PDS (7.5 mM) and acidic conditions (pH=3–7) were beneficial for NAP degradation. In soil, both CAT and AA promoted PDS activation and NAP degradation. Low soil organic matter and high Fe/Mn-mineral contents were favorable for NAP degradation by AA-activated PDS. Column experiments confirmed that NAP was readily transported and degraded under porous medium conditions using AA-activated PDS. Moreover, we revealed that SO 4 •− and HO• were the dominant reactive species for NAP degradation by AA-activated PDS. Intermediate products of NAP in the AA-activated PDS process were analyzed and the reactive sites of NAP were predicted. E. coli growth tests verified that the intermediate products in the AA-activated PDS process were less toxic than NAP. Our results highlight the high potential of NOAs-activated PDS process for the remediation of NAP-contaminated water and soil.
研究领域:
环境科学
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化学工程
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毒理学
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PubMed
高影响因子
ELECTROCHIMICA ACTA
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2023
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IF 6.6
Junhong Li,Danhua Zhu,Hui Huang,Shuqian Xie,Jingkun Xu,Ruirui Yue,Xuemin Duan
Herein, the electrochemical sensing platform has been proposed based on metal-organic framework doped Au/poly(3,4-ethylenedioxythiophene) (PEDOT) composites to simultaneously detect the catechin and sunset yellow in tea beverage. The characterization reveals that the Au nanoparticles distribute well on/in conducting PEDOT, and the relatively few UIO-66 particles as the dopant intersperse the Au/PEDOT composites. The electrochemical test has been used to study the sensing properties toward catechin and sunset yellow simultaneous detection. The results show that the Au/PEDOT/MWCNT-CONH-UIO-66 performs a wide range of 0.03∼60 µM and 0.3∼40 µM, low detection limit as 0.01 μM / 0.15 μM towards catechin and sunset yellow simultaneous detection, respectively. Furthermore, the Au/PEDOT/MWCNT-CONH-UIO-66 possesses a wide linear detection range and high sensitivity for catechin and sunset yellow detection. Moreover, the Au/PEDOT/MWCNT-CONH-UIO-66 composite also has great stability and anti-interference ability. Finally, the sensing platform was applied to detect catechin and sunset yellow in the tea beverage compared with the high-performance liquid chromatography method. To the best of our knowledge, our work first reported the electrochemical sensing materials for catechin and sunset yellow simultaneous detection in food, which will provide a certain theoretical analysis and experimental experience for the preparation of materials used for catechin and sunset yellow simultaneous detection in food.
研究领域:
食品科学
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电化学传感
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材料科学
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高影响因子
TALANTA
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2022
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IF 6.556
Yang Hu,Chenxi Wang,Pengcheng Zhao,Li Zhang,Junjie Fei,Yixi Xie
In this paper, the precursor ZIF-8/NaCl composite material was successfully synthesized by the in-situ synthesis method, and the two-dimensional zinc doped carbon nanosheets (Zn CNs) were obtained after heat treatment of the precursor. Zn CNs were electrodeposited on carboxylated carbon nanotubes (MWCNTs-COOH) modified glass carbon electrode (GCE) to construct an electrochemical sensor for catechins. The materials are characterized using scanning electron microscope, X-ray photoelectron spectroscopy, and nitrogen adsorption-desorption isotherm. Zn CNs/MWCNTs-COOH composite provide the sensor with excellent electrochemical and electrocatalytic performance. Therefore, the sensor has a wide detection range (30.0–738 nM) and a low detection limit (LOD = 10.0 nM) for catechins. The effectiveness of the sensor was also verified in actual sample detection with good stability and accuracy. This study may provide a feasible solution for the detection of catechin.
研究领域:
材料科学
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分析化学
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DOI
高影响因子
FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Dengke Xie,Hong Hu,Qingrong Huang,Xuanxuan Lu
Bigels are system with great potential in 3D printing and bioactive compound delivery. In this study, 3D printed bigels based on candelilla wax oleogel and gelatin hydrogel were developed as delivery system for hydrophilic and lipophilic bioactives (namely, quercetin and catechin). Bigels with monoglyceride or lecithin formed stable gel system with 3D printing ability. Microstructural study found that changing of oleogel/hydrogel ratios (3:7, 5:5, and 7:3) under the presence of different emulsifiers (monoglyceride and lecithin) led to structural transformation of bigels from oleogel-in-hydrogel structure to hydrogel-in-oleogel or bicontinuous status. Low frequency-NMR analysis indicated that the signals of typical T 2 relaxation peak were changed accordingly with variations of oleogel/hydrogel ratio and using of different emulsifiers. XRD patterns of bigels revealed their crystalline peaks grew with increase of oleogel content. In vitro digestion study found that both oleogel/hydrogel ratio and emulsifiers used exhibited large influence on the lipolysis extent, release rate of quercetin and catechin from bigels. The highest release rate for catechin (53.37%) and quercetin (11.08%) were observed in bigels containing monoglyceride with 70% oleogel and lecithin with 30% oleogel after simulated digestion, respectively. This study provided valuable guidance for developing bigels as 3D printed food with potential in co-delivery of hydrophilic and lipophilic bioactives by adjusting oleogel/hydrogel ratios and emulsifiers used.
研究领域:
食品科学
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生物工程
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2013
·Vol. 14
·期 1
·1802-1821
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IF 2.464
Rongxin Qin,Kangkang Xiao,Bin Li,Weiwei Jiang,Wei Peng,Jiang Zheng,Hong Zhou
Fructus crataegi (hawthorn) is the common name of all plant species in the genusCrataegusof the Rosaceae family. In the present study, three monomers of (+)-catechin (C), (−)-epicatechin gallate (ECg) and (−)-epigallocatechin (EGC) were isolated from the hawthorn under the guide of antibacterial sensitization activity. The bioactivity of the composite fraction in enhancing the antibacterial effect of oxacillin against methicillin-resistantStaphylococcus aureus(MRSA) was greater than that of the individual monomer of the hawthorn extractin vitro. Two-fold dilution and checkerboard methods were used to analyze antibacterial activity and screen for the combination and proportion of monomers with the best bioactivity. The result showed that C (128 mg/L) combined with ECg (16 mg/L) had the greatest effect and the combination also reduced the bacterial load in blood of septic mice challenged with a sublethal dose of MRSA, increased daunomycin accumulation within MRSA and down-regulated the mRNA expression ofnorA,norCandabcA, three important efflux pumps of MRSA. In summary, C and ECg enhanced the antibacterial effect of β-lactam antibiotics against MRSAin vitroandin vivo, which might be related to the increased accumulation of antibiotics within MRSA via suppression of important efflux pumps’ gene expression.Keywords:fructus crataegi;(+)-catechin;(−)-epicatechin gallate;synergistic effect;MRSA;drug accumulation
研究领域:
药理学
·
微生物学
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植物化学
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传染病学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Silei Zhang,Qingbo Huang,Xiangmin Kuang,Chaofan Guo,Yongli Jiang,Fujie Zhang,Sakamon Devahastin,Junjie Yi
Oxidative polymerization of catechins drove the green-to-brown color transition. • Electrolyzed water with ultrasound improved drying efficiency and flavor retention. • More extensive cellular disruption contributed to accelerated moisture migration. • Color, aroma, and moisture changes closely reflected quality changes during drying. • Artificial neural network most accurately predicted moisture and catechin contents. Color is critical determinant of market grade and commercial value of star anise ( Illicium verum Hook. f.). However, mechanisms underlying its characteristic green-to-reddish-brown transformation during processing remain insufficiently understood. The present study investigated color evolution of star anise by comparing ultrasound-assisted electrolyzed water treatment (UEWT) with conventional hot-water blanching (HWB) using framework combining drying kinetics, E-nose, LF-NMR, untargeted LC-MS data as well as machine learning modeling. UEWT significantly enhanced drying efficiency, shortening drying time to 8 h compared to 13 h in case of HWB. LF-NMR spectra revealed that UEWT maintained distinct water mobility profiles, effectively reducing internal mass transfer resistance. UEWT also intensified the chromatic transition, as evidenced by a decline in L* , rapid increases in a* and BI, a greater Δ E , an overall decrease in H , and higher late-stage C* values than those observed under HWB. Catechin depletion, accompanied by formation of oligomeric oxidation products, represents key transformation pathway associated with color development. Among the evaluated machine learning models, ANN-based model exhibited superior performance, achieving high prediction accuracy for both moisture content ( R 2 > 0.98) and catechin content ( R 2 > 0.92). These findings provide new insights into physicochemical basis of color formation and offer practical strategy for real-time quality control during industrial drying of aromatic crops. Download: Download high-res image (294KB) Download: Download full-size image
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Tong Zhang,Yuan Chen,Tao Wang,Chang Liu,Dan He,Bin Liu,Yuanyuan Liu
Advanced oxidation processes are widely used to remove petroleum hydrocarbons from soil, but usually consume large quantities of ferrous and acidify the soil. This study tested an advanced oxidation approach based on percarbonate in laboratory experiments. It removed 88% petroleum hydrocarbons in soil with a pH increase from 8.2 to 10.2. ·OH and ·CO 3 - were the main reactive species, and degraded 41% and 37% PHCs from soil respectively. The o-dihydroxybenzene structure in catechin was found to reduce ferric to ferrous, and prolong the generation of ·OH from 120 s to over 1800 s. The petroleum hydrocarbons were degraded to intermediates including alkanes and olefins through hydrogen-abstraction by ·OH and ·CO 3 - , and by dimerization and β-scission of alkyl radicals. These intermediates were then oxidized to CO 2 and H 2 O by ·OH and ·CO 3 - . The main residual intermediates in the soil were low-molecular-weight n-alkanes and branched alkanes, and they were found to inhibit the growth of oats ( Avena sativa L. ) much less than the original petroleum hydrocarbons. These findings provide a fundamental basis for designing effective technologies which use percarbonate to remove organic pollutants .
研究领域:
环境科学
·
土壤修复
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hai He,Fanrui Liu,Lei Yang,Honghao Fan,Hao Wan
This paper highlighted the structural changes of rice starch complexed with green tea catechin and lemon peel caffeic acid under thermomechanical-assist low moisture, and their prebiotic functions during in vitro digestibility and fermentation were further explained. Extruded starch with caffeic acid, characterized by enhanced short-range order, a distinct fractal structure, and a V-amylose helical arrangement, exhibited slower glucose release due to V-type inclusion crystalline structures and strong hydrogen bondings. Additionally, extruded starch with catechin or caffeic acid changed the composition of gut microbiota by increasing the proliferation of good bacteria and reducing pathogenic microorganisms, which led to a greater synthesis of short-chain fatty acids. According to a PICRUSt2 analysis, the extruded starch with caffeic acid may trigger metabolic alterations via altering the gut flora and increasing bile acid metabolism. Thus, extruded starch with caffeic acid demonstrates significant potential as a prebiotic for developing nutritionally tailored starch-based foods.
研究领域:
食品科学
·
微生物学
·
营养学
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DOI
PubMed
高影响因子
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