查找使用阿拉丁产品的科研文献

Chain conformation, mucoadhesive properties of fucoidan in the gastrointestinal tract and its effects on the gut microbiota

Aijun Chen,Yatong Liu,Tao Zhang,Yu Xiao,Xiaoqi Xu,Zheng Xu,Hong Xu

Fucoidans are valuable marine polysaccharides with various bioactivities and physicochemical properties. However, its digestive properties, mucoadhesive properties, and bioactivity in the gastrointestinal tract are still unclear. In this study, simulated digestion, fecal fermentation in vitro , and rheology models were utilized to investigate the chain conformation, influence on gut microbiota , and mucin adhesive properties of fucoidan from the sea cucumber Thelenota ananas ( Ta -FUC). The results showed that Ta -FUC was nondigestible with a temporary decrease in molecular weight in gastric conditions, accompanied by the chain conformation becoming more flexible. Moreover, Ta- FUC exhibited strong mucin adhesive function in the simulated intestinal environment, with supramolecular disulfide , hydrogen, and hydrophobic interactions in order of intensity. During fermentation, Ta -FUC was degraded by the intestinal flora to produce various short-chain fatty acids and promoted the relative abundance of Bacteroidota and Firmicutes , reducing the proportion of Proteobacteria . Therefore, these results indicate that Ta -FUC could be a potential prebiotic and ingredient for developing targeted delivery systems in the functional food and pharmaceutical industries .

Photothermal and Acid-Responsive Fucoidan-CuS Bubble Pump Microneedles for Combined CDT/PTT/CT Treatment of Melanoma

Jiaojiao Tao,Bingjie Wang,Yu Dong,XiangYan Chen,Shuang Li,Tianze Jiang,Xia Zhao

Transdermal cancer therapy faces great challenges in clinical practice due to the low drug transdermal efficiency and the unsatisfactory effect of monotherapy. Herein, we develop a novel bubble pump microneedle system (BPMN-CuS/DOX) by embedding sodium bicarbonate (NaHCO3) into hyaluronic acid microneedles (MNs) loaded with fucoidan-based copper sulfide nanoparticles (Fuc-CuS NPs) and doxorubicin (DOX). BPMN-CuS/DOX can generate CO2 bubbles triggered by an acidic tumor microenvironment for deep and rapid intradermal drug delivery. Fuc-CuS NPs exhibit excellent photothermal effect and Fenton-like catalytic activity, producing more reactive oxygen species (ROS) by photothermal therapy (PTT) and chemodynamic therapy (CDT), which enhances the antitumor efficacy of DOX and reduces the dosage of its chemotherapy (CT). Simultaneously, DOX increases intracellular hydrogen peroxide (H2O2) supplementation and promotes the sustained production of ROS. BPMN-CuS/DOX significantly inhibits melanoma both in vitro and in vivo by the combination of CDT, PTT, and CT. In short, our study significantly enhances the effectiveness of transdermal drug delivery by constructing BPMNs and provides a promising novel strategy for transdermal cancer treatment with multiple therapies.

Depolymerized Fucoidan Alleviates High-Fat Diet-Induced Obesity in Association with Alterations in Gut Microbiota and Metabolic Profiles

Obesity is a major global health concern associated with various metabolic disorders. Fucoidan, a seaweed-derived sulfated polysaccharide, has shown potential in alleviating obesity through modulation of gut microbiota. However, comparative effects of native and depolymerized fucoidan (Dfuc) on HFD-induced obesity and gut microbiota dysbiosis remain unclear. In this study, we compared the anti-obesity effects of fucoidan and Dfuc and evaluated their regulatory effects on gut microbiota and microbial metabolites in HFD-fed mice. Results showed that fucoidan and Dfuc reduced body weight gain, fat accumulation and hepatic lipid abnormalities. They also alleviated liver injury and oxidative stress and inflammation in hepatic and colonic tissues, with Dfuc significantly improving parameters such as serum AST, hepatic MDA, SOD activity, and TNF-α expression compared to the HF group. Furthermore, gut microbiota analysis showed that both fucoidan and Dfuc treatments enrichedAlloprevotellaandAllobaculum, while fucoidan increasedAkkermansiaandBlautia, and Dfuc promotedBifidobacteriumandRomboutsia. Moreover, metabolite analysis showed that Dfuc significantly increased fecal acetate and propionate concentrations, and induced more pronounced changes in fecal metabolites related to amino acid, carbohydrate, and bile acid metabolism. These findings suggest that Dfuc holds potential as a functional food ingredient for alleviating HFD-induced obesity and gut microbiota dysbiosis.

Hesperetin loaded fucoidan selenium nanoparticles alleviate cisplatin induced acute kidney injury via antioxidant and cGAS STING pathway regulation

Long Lin,Zhang Yexi,Xiao Jun,Zang Jianhua

Purpose To fabricate hesperetin-loaded fucoidan-selenium (Hes/Fu-Se) nanoparticles (NPs) for alleviating cisplatin-induced acute kidney injury (AKI), overcoming hesperetin’s low solubility and bioavailability and boosting antioxidant and anti-inflammatory efficacy. Methods Hes/Fu-Se NPs were prepared by encapsulating hesperetin within fucoidan, and then functionalized selenium (Se) NPs. Nanoparticle physicochemical properties and antioxidant activity (FRAP, ABTS) were characterized. Cisplatin-stimulated HK-2 cells were used for in vitro detection of cell viability, apoptosis, ROS accumulation, mitochondrial function and cGAS-STING signaling. A cisplatin-induced AKI mouse model was adopted to test renal biochemical indexes, renal histopathology and renal injury-related protein expression. Results Synthesized Hes/Fu-Se NPs exhibited uniform particle size, superior aqueous solubility and potent antioxidant activity. In vitro, the NPs restored HK-2 cell viability, mitigated apoptosis and ROS overproduction, and inhibited DNA damage and cGAS-STING cascade activation. In vivo, Hes/Fu-Se markedly reduced serum BUN and CRE, alleviated renal histological lesions and inflammatory infiltration, and downregulated the expression of cGAS, STING, γH2AX and TNF-α in kidney tissues. Conclusions Hes/Fu-Se NPs relieve cisplatin-triggered AKI via amplified antioxidation, DNA damage restoration and cGAS-STING pathway suppression. This nanoformulation provides a promising nephroprotective strategy against chemotherapy-related renal toxicity for clinical translation. Graphical abstract

Low-molecular-weight fucoidan prepared by ultrasonic/H2O2/VC degradation: Characterization, gut microbiota regulation, and anti-inflammatory effects

Huadan Zhang,Congcong Sun,Kailin Li,Xianliang Luo,Zebin Guo,Song Miao,Baodong Zheng

Fucoidan, a marine-derived sulfated polysaccharide, possesses a diverse range of bioactive properties. This study aimed to evaluate the physicochemical characteristics and anti-inflammatory activity of Laminaria japonica fucoidan (LJF) and degradation fractions (DLJF-Ts) obtained through ultrasonic-assisted H 2 O 2 /Vitamin C (VC) treatment (1, 3, and 6 h). The molecular weights of LJF, DLJF-1, DLJF-3, and DLJF-6 were 225, 101, 45, and 31 kDa, respectively. The fucose was the major monosaccharide, and structural analysis demonstrated that the main functional groups were unchanged, while their sheets changed from larger to smaller. Meanwhile, the DLJF-3 showed the superior effects of in vitro antioxidant and anti-inflammatory. Furthermore, after 48 h of colitis mice fecal fermentation, the DLJF-3 was metabolized by gut microbiota, leading to a marked elevation in short-chain fatty acids. The content of Bacteroides increased and the Desulfovibrio reduced significantly. Overall, the obtained results revealed that DLJF-3, as the anti-inflammatory agent, could be used for the development of functional foods and healthcare products.

Dual-responsive gelatin/fucoidan-based membrane with colorimetric and control-released performance for meat freshness monitoring and preservation

Linlin Li,Haoran Wang,Yimeng Zhang,Wei Ji,Minmin Chen,Baocai Xu,Hualin Wang

Food packaging combined with active and intelligent properties plays an essential role in minimizing food waste and guaranteeing food quality. Herein, we developed a colorimetric and control-released dual-responsive nanofiber membrane (GF-AE-LI) with gelatin/fucoidan (GA/FU, i.e., GF) as matrix, aloe-emodin (AE) as indicator and limonene (LI) as antibacterial entity by electrospinning. The results exhibited that an appropriate combination of GA and FU based on the electrostatic interaction was conducive to constructing a carrier of GF in response to an alkaline atmosphere. The AE and LI were encapsulated in GF nanofibers with high efficiency and well-kept structure. Moreover, the membrane was capable of pH-controlled release of LI by a non-Fickian diffusion mechanism and strong antibacterial effects against Staphylococcus aureus and Escherichia coli . Furthermore, the membrane can effectively extend the shelf life of chicken breast meat by 2 d, and its pH-colorimetric changes were highly correlated with the spoilage indices (Pearson′s r = 0.9554), demonstrating the potential of GF-AE-LI toward simultaneously freshness monitoring and preservation of food.

The characterization of fucoidan‑sodium caseinate electrostatic complexes with application for pH-triggered release: Microstructure and digestive behavior

Tao Zhang,Ruowei Huang,Xiaoqi Xu,Yaoguang Chang,Changhu Xue

This study was aimed at enhancing the stability of the medium internal phase emulsion by modulating the electrostatic interaction between fucoidan (FUC) and sodium caseinate (NaCS). Firstly, the macro and microstructure of FUC-NaCS electrostatic complexes with the mass ratio of ranged from 0.01: 2 to 4: 2 were examined at pH 3.0. As the concentration of FUC increased, the complexes transformed a soluble state to phase separation and then returned to the homogeneous state, with the turbidity and particle size initially increasing and then decreasing. The zeta potential of the complexes gradually declined, and the zero-point was corresponding to the phase separation region where the ratio of FUC-NaCS was around 0.75: 2. The microstructure by TEM and SEM demonstrated that the NaCS particles were bonded to the FUC cross-linking chain network via electrostatic interaction to form self-assembling complexes. Subsequently, the simulated digestion results suggested that the FUC-NaCS complexes could delay the hydrolysis of NaCS and displayed greater stability in gastric environments. Based on the above results, the FUC-NaCS complexes were utilized to emulsify and stabilize medium chain triglycerides (MCT). The droplets of the FUC-NaCS emulsion at 4: 2 was more dispersed with a smaller diameter, and also exhibited superior stability during stomach digestion and concurrently high digestibility during intestinal digestion, making FUC-NaCS complexes a potential choice for pH-triggered release of emulsions, especially in gastrointestinal environment.

Naringenin loaded fucoidan/polyvinylpyrrolidone nanoparticles protect against folic acid induced acute kidney injury in vitro and in vivo

Tao Jiang,Feikai Zhu,Xintao Gao,Xiaochen Wu,Wenyong Zhu,Chuanlong Guo

Acute kidney injury (AKI) is a common clinical problem with no effective treatment. Excessive folic acid (FA) induced kidney tubular injury is characterized by oxidative stress and inflammation, and is a common model of AKI. The excellent pharmacological activity of naringenin (NAR) makes it a potential agent for treating AKI, but its poor solubility limits its application. This study prepared NAR loaded nanoparticles (FU/PVP-NAR) using fucoidan (FU) and polyvinylpyrrolidone (PVP) as carriers, with a particle size of 23.96 ± 2.77 nm. In vitro studies showed that FU/PVP-NAR inhibited excessive FA induced proliferation inhibition, accumulation of reactive oxygen species (ROS), and disruption of mitochondrial membrane potential (MMP) of HK-2 cells. Further confirmed that FU/PVP-NAR inhibited FA induced DNA damage and Cyclic GMP–AMP synthase (cGAS)-stimulator of interferon genes (STING) activation. In vivo studies showed that excessive FA induced AKI features in mice, such as elevated serum creatinine (SCr) and blood urea nitrogen (BUN) levels, accompanied by pathological damage to kidney tissues. The above AKI characteristics induced by FA were alleviated by FU/PVP-NAR. FU/PVP-NAR also inhibited the decrease in antioxidant enzyme levels in kidney tissues induced by FA. Furthermore, in vivo mechanism studies indicated that FU/PVP-NAR inhibited the release of inflammatory factors by inhibiting DNA damage-cGAS-STING pathway. In summary, this study provided the possibility for FU/PVP-NAR as a potential candidate drug for treating FA induced AKI.

ZE21B magnesium alloy coimmobilized with anti-inflammatory chemerin 15 peptide and pro-endothelialization fucoidan for enhanced vascular histocompatibility

Yuwei Gu,Qi Wang,Shaosheng Jia,Jianan Yu,Lan Chen,Lingchuang Bai,Shaokang Guan

Magnesium alloy stents (MAS) show remarkable application potential for treating cardiovascular diseases, attributable to their superior mechanical properties and biodegradability. However, their clinical applications are significantly hindered by inadequate corrosion resistance and suboptimal blood compatibility. In this study, a multilayer coating was constructed on the Mg-Zn-Y-Nd (ZE21B) alloy to enhance its degradation rate, blood compatibility, and the capacity to promote endothelialization in the field of vascular stent applications. This coating comprised layers of magnesium fluoride, polydopamine, fucoidan, and the chemerin 15 (C15) peptide, achieved through a surface modification technique. The modified ZE21B alloy exhibited increased charge transfer resistance and decreased corrosion current density, suggesting a marked enhancement in its corrosion resistance. Furthermore, the modified alloy demonstrated exceptional blood compatibility, as evidenced by the comparatively low magnitudes of hemolysis, fibrinogen adsorption and denaturation, and platelet adhesion. After fucoidan and C15 peptide were grafted on samples, the coating notably promoted key biological behaviors of endothelial cells (ECs), including adhesion, proliferation, migration, and nitric oxide (NO) release. In contrast, it could efficiently restrain smooth muscle cell adhesion and proliferation. The ECs on the fucoidan and C15 peptide modified ZE21B alloy exhibited obvious competitive growth advantages over SMCs in a co-culture system. Furthermore, the introduction of the C15 peptide had endowed the ZE21B alloy with anti-inflammatory properties through regulating the M2 polarization of macrophages. This study offered a highly promising approach for the development of degradable MAS endowed with outstanding corrosion resistance and vascular histocompatibility.

Sargassum fusiforme fucoidan modifies gut microbiota and intestinal metabolites during alleviation of hyperglycemia in type 2 diabetic mice

Qifang Wu,Siya Wu,Yang Cheng,Zhongshan Zhang,Genxiang Mao,Shijun Li,Yue Yang,Xu Zhang,Mingjiang Wu,Haibin Tong

Type 2 diabetic mellitus (T2DM) is a complicated metabolic disorder that is now considered as a major global public health problem. Fucoidan exhibits diverse biological activities, especially prevention of metabolic diseases. In this regard, we herein aimed to reveal the beneficial effect of Sargassum fusiforme fucoidan (SFF) on high-fat diet (HFD) and streptozotocin (STZ) induced T2DM mice. We noted that on the one hand, SFF significantly decreased fasting blood glucose, diet and water intake, and hyperlipidemia, while on the other hand, it improved glucose tolerance. Furthermore, SFF reduced epididymal fat deposition, attenuated the pathological changes in heart and liver tissues, and decreased oxidative stress in diabetic mice. To explore the underlying mechanisms of these ameliorative effects, the gut microbiota was analyzed. Notably, SFF highly enriched benign microbes including Bacteroides, Faecalibacterium and Blautia, as well as increased levels of (R)-carnitine and choline in the colon of diabetic mice. This may be a potential mechanism for alleviating T2DM, thus implying the benefits of SFF as an adjuvant agent for T2DM treatment. Taken together, this study demonstrated a promising application of fucoidan as one of the adjuvant agents for the management of T2DM in the future.

Fucoidan/κ-carrageenan mixed gel: Effect of anions of different valence including chloride, bromide, iodide, sulfate and phosphate

Nan Wang,Jie Tian,Li Guo,Xueting Chen,Bingbing Hu,Shuang Song,Chengrong Wen

The effects of the differently charged anions, NaCl, NaBr, NaI, Na 2 SO 4 , and Na 3 PO 4 on the gel properties and structure of the fucoidan/κ-carrageenan mixed gel (FC) were investigated using the rheometer, texture analyzer, LF-NMR, Cryo-SEM, FT-IR, and XRD. At a certain concentration, Cl − and Br − could stabilize the helical conformation of the gel through electrostatic shielding. The addition of SO 4 2− and PO 4 3− would reduce the strength of the gel. And I − would soften the gel. With increasing anion concentration, the electrostatic repulsion between FUC and KC was gradually enhanced. The FUC dispersions remained liquid after the addition of anions, while FC gelled depending on the type and concentration of anions. The elastic modulus increased with increasing concentration of NaCl and NaBr, but decreased with increasing concentration of NaI, Na 2 SO 4 , and Na 3 PO 4 . Moreover, the addition of anions promoted the water migration of FC, as reflected by the T 23 relaxation time changing from high to low and the significantly higher migration of NaCl and NaBr than NaI and Na 2 SO 4 . The pores of the gel microstructure change from disordered to uniform. The FT-IR and XRD results were well related to the structural changes. There is electrostatic repulsion between anion and FC, and the binding ability of FC to monovalent anions and trivalent anions is better than that of divalent anions. Overall, this study could provide a theoretical basis for the design and development of FUC-based gels.

Preparation and evaluation of ovalbumin-fucoidan nanoparticles for nicotinamide mononucleotide encapsulation with enhanced stability and anti-aging activity

Shan Sun,Xuedi Zhang,Jiaxuan Li,Yu Li,Chengfu Zhou,Siyuan Xiang,Mingqian Tan

Nicotinamide mononucleotide (NMN) has been recognized as a promising bio-active compound in relieving aging-related mitochondrial dysfunction. Self-assembled nanoparticles were prepared based on interaction between ovalbumin (OVA) and fucoidan to improve the stability and bio-accessibility of NMN. The OVA-fucoidan nanoparticles (OFNPs) displayed outstanding thermal stability and entrapment ability of NMN. The reactive oxygen species (ROS) analysis and senescence-associated β-galactosidase (SA-β-gal) staining characterization indicated that NMN encapsulated by OFNPs could effectively alleviate the cellular senescence of d -galactose-induced senescent cells. In vivo Caenorhabitis elegans experiment demonstrated that NMN-loaded OFNPs caused less accumulation of lipofuscin and protected NMN from thermal damage. Compared with free NMN, the NMN-loaded OFNPs prolonged lifespan from 28 to 31 days, increased 26% reproductive ability, and improved 12% body length of Caenorhabitis elegans . The results indicated that the use of nanocarriers could be a good strategy to improve anti-oxidative stress and anti-aging ability of NMN.