NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 37
·16546-16556
·
IF 2.7
Xiubin Ren,Chun Huang,Zhengxuan Gao,Ruichen Zhai,Youyu Zhu,Yingfeng Duan,Linyong Chen,Weiqin Zhao,Guofu Li
The presence of phenol in coal-chemical engineering wastewater is considered a serious environmental issue. The use of enzymes to degrade phenol has received wide attention. G-quadruplex can combine with hemin to form G-quadruplex/hemin, which shows peroxidase-like activity and can be used to degrade phenol. In this work, a novel CNTs-NH2@G-quadruplex/hemin was designed using a single-stranded DNA (ssDNA) that contained G-rich fragments and T-rich fragments. The G-rich fragments assembled with the hemin to form G-quadruplex/hemin; the pyrimidine group of the T-rich fragment could form stable chemical bonds with the amino group, which could be used for immobilization. Characterization analysis of FTIR, XRD and TEM showed that the CNTs-NH2@G-quadruplex/hemin was successfully prepared. Meanwhile, the activity and phenol degradation ability for CNTs-NH2@G-quadruplex/hemin were studied in detail, and the effects of pH, temperature, initial phenol concentration, H2O2 concentration and reusability on the degradation efficiency of phenol were also explored. The results showed that when immobilized, the CNTs-NH2@G-quadruplex/hemin presented good activity, especially under conditions of temperature 30 °C and pH 7.0. Notably, the maximum reaction velocity of CNTs-NH2@G-quadruplex/hemin was increased from 0.016 mM mg−1 min−1 (free enzyme) to 0.056 mM mg−1 min−1. For phenol degradation ability, it was observed that under the conditions of temperature 30 °C, pH 7.0, initial phenol concentration 40 mg L−1, H2O2 concentration 120 mM, enzyme dose 40 mg L−1, more than 90% of phenol could be degraded within 600 min. In addition, the CNTs-NH2@G-quadruplex/hemin still maintained high activity after 10 cycles of phenol removal, demonstrating its appealing potential for practical applications.
研究领域:
环境科学
·
生物化学
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DOI
高影响因子
INORGANIC CHEMISTRY
·
2026
·
IF 4.6
Mengjun Xiao,Yu Gui,Andraž Mavrič,Yao Nie,Wendong Zhang,Peng Chen,Pengpeng Wang,Yi Wang
The chlorine evolution reaction (CER) is essential for industrial chlorine production. However, achieving high CER selectivity remains a challenge due to the high cost and narrow potential gap between the CER and the competing oxygen evolution reaction (OER) in conventional noble oxide catalysts. In this work, Hemin is adopted as a model molecular catalyst for CER owing to its porphyrin macrocycle and the axial Cl ligand, achieving an overpotential of ∼190 mV at 10 mA cm–2 with ∼100% chlorine selectivity and excellent stability over 100 h in corrosive acidic brine. Spectroscopic and electrochemical analyses reveal that the axial Cl–Fe interaction stabilizes the rigid configuration geometry of the Hemin molecule, while the π-conjugated macrocycle and the Fe-centered electronic effect jointly modulate the electronic state of Fe. This combination effectively suppresses electrochemical demetalation and preserves structural integrity for enhancing selectivity and durability. It is hoped that this work can propose fundamental insight into the rational design of efficient molecular electrocatalysts for promoted chlorine evolution.
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Wencheng Xiao,Hang Ao,Nilin Wu,Jun Sun,Wenrui Hu,Yunlong Chen,Jie Wu,Huangxian Ju
Highly sensitive detection of cell secretions at the single-cell level is critical for efficient cell screening and monoclonal antibody (mAb) production. Herein, we present a highly sensitive microfluidic chemiluminescence immunoimaging (μCLII) method for rapid and quantitative screening of high-yield specific hybridoma cells (HSHCs) by detecting single-cell secreted antibodies on a rationally designed lantern-shaped microchamber array chip. This method can effectively capture and isolate single hybridoma cells to in situ detect their secreted antibody by visual CL imaging with a proximity recognition amplification strategy. The proposed strategy can be performed by antibody-triggered proximity hybridization to release a DNA sequence, which initiates a rolling circle amplification reaction to turn on abundant hemin-DNA enzymes for generating strong CL emission of the imidazole-enhanced luminol-H2O2 system. This method shows a detection limit of ∼60 antibodies per chamber, and thus enables a precise sorting of HSHCs within 70 min. Using antiproprotein convertase subtilisin/kexin type 9 mAb (PCSK9-mAb) as a model, all of the cells selected by the μCLII method show high-producing hybridomas with PCSK9-mAb yield more than 80 fg during the screening process, indicating a promising potential of the proposed method for single-cell analysis and mAbs screening.
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PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Huyue Wei,Mingzhu Yang,Chengshuai Huang,Xiaojie Yue,Jiabin Cai,Xinyi Wang,Kai Fan,Linxi Dong,Gaofeng Wang,Dujuan Li
Neuron-specific enolase (NSE), a tumor marker of small cell lung cancer (SCLC), has high application value in the early diagnosis of SCLC. In this study, a dual signal electrochemical aptasensor for NSE was constructed based on hemin/reduced graphene oxide/multi-walled carbon nanotube (H–rGO–MWCNT) nanocomposites. Hemin played a dual role, functioning not only as an in situ electrochemical probe but also exhibiting excellent peroxidase-like properties, effectively catalyzing the electroreduction of H2O2. Reduced graphene oxide and multi-walled carbon nanotubes exhibited excellent conductivity. Through their binding with hemin, the nanocomposites achieved a larger specific surface area, providing numerous active sites for capturing the NSE aptamer. In the presence of NSE, the specific adsorption between the antigen and the aptamer formed a stable antigen–aptamer structure, which inhibited the performance of hemin, resulting in the weakening of the electrochemical signals of hemin and H2O2. Leveraging these characteristics, the sensitive and cost-effective dual-signal electrochemical aptasensor has been fabricated for the detection of NSE. One signal corresponded to differential pulse voltammetry (DPV) of hemin, while the other signal was derived from chronoamperometry, capturing the catalytic reduction of H2O2. The linear ranges for NSE were 1 pg mL−1 to 1 μg mL−1 and 100 pg mL−1 to 100 ng mL−1 with the limit of detection (LOD) of 0.21 pg mL−1 and 11.22 pg mL−1 by DPV and chronoamperometry, respectively. In addition, this aptasensor exhibited good reproducibility, stability and specificity. The recovery of NSE in human blood serum samples was from 89% to 131%. It provided a promising strategy for the detection of NSE in clinical diagnostics.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Qian Yu,Yongmei Wu,Zi Liu,Sheng Lei,Gaiping Li,Baoxian Ye
This work designed an artificial substrate peptide to synthesize peptide-hemin/G-quadruplex (peptide-DNAzyme) conjugates . In addition to enhancing catalytic activity of hemin/G-quadruplex, the peptide could also be induced and cleaved by prostate specific antigen (PSA). It was the first report on peptide-DNAzyme conjugates in application of the peptide biosensor. The polyethyleneimine-reduced graphene [email protected] platinum nanotubes ( [email protected] ) nanocomposites were cast on the glassy carbon electrode in order to form the interface of biocompatibility and huge surface area for bioprobes immobilization . In absence of PSA, the peptide-DNAzyme conjugates retained intact on the surface of the electrode to produce a strong response signal. But in presence of PSA, the peptide-DNAzyme conjugates were destroyed to release electron mediators, resulting in dramatical decrease of the electrochemicl signal. Therefore, the method had high sensitivity and super selectivity with the limit of detection calculated as 2.0 fg/mL. Furthermore, the strategy would be promising to apply for other proteases by transforming the synthetic peptide module of target.
研究领域:
生物化学
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分析化学
·
生物传感器
·
癌症诊断
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-11
·
IF 6.232
Zhou Zhide,Zhao Le,Li Wenzhan,Chen Min,Feng Huafu,Shi Xiaohang,Liang Jintao,Li Guiyin
A Glypican-3 (GPC3) electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids (HGNs) peroxidase-like catalytic silver deposition and GPC3 aptamer has been constructed for the determination of GPC3. The HGNs were prepared by an one-step reduction method. Fourier transform infrared spectroscopy (FT-IR), ultraviolet spectroscopy (UV-vis), and transmission electron microscopy (TEM) were used to study the structure and morphological characteristics of the HGNs. The GPC3 electrochemical aptamer nanobiosensor was constructed using HGNs-aptamer (HGNs-Apt) as the signal probe and GPC3 aptamer as the capture probe. With the help of the catalytic action of peroxidase-like properties of HGNs, H 2 O 2 reduces the silver (Ag) ions in solution to metallic Ag, which deposit on the surface of the electrode. The amount of deposited Ag, which was derived from the amount of GPC3, was quantified by differential pulse voltammetry (DPV). Under optimal conditions, the current response of Ag had a good positive correlation with the GPC3 concentration in the range 10.0–100.0 μg mL −1 with a correlation coefficient of 0.9958. The detection limit was 3.16 μg mL −1 at a signal-to-noise ratio of 3, and the sensitivity was calculated to be 0.807 μA μM −1 cm −2 . The method is validated by analyzing spiked human serum samples with good recovery ranging from 101 to 122%. In addition, the GPC3 electrochemical aptamer nanobiosensor has acceptable selectivity, stability, and reproducibility.
A Glypican-3 electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids (HGNs) peroxidase-like catalytic silver deposition and GPC3 aptamer has been constructed for the determination of GPC3. The electrochemical aptamer nanobiosensor exhibits high selectivity, acceptance reproducibility, and good recovery performances.
研究领域:
生物传感器
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分析化学
·
纳米技术
·
癌症诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Xiaodong Cao,Zihao Xia,Wuwen Yan,Shudong He,Xuan Xu,Zhaojun Wei,Yongkang Ye,Haisong Zheng
In this paper, we present a simple and label-free colorimetric biosensor for detection of the nopaline synthase (NOS) terminator in genetically modified (GM) plants. The “signal on” colorimetric biosensor was developed using a nanocomposite consisted of gold nanoparticles doped magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 @Au NP), capture probe DNA (cDNA), and hemin-functionalized reduced graphene oxide nanosheets (H-GN). The nanocomposite was successfully prepared by means of Au–S bonds and the strong π interactions between cDNA and H-GN. The sensing approach is based on the excellent peroxidase-mimicking activity of H-GN and its different electrostatic interactions with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). In presence of the target NOS, the cDNA in the nanocomposite will hybridize with its complementary sequence, and form dsDNA structure. Due to the weak π interactions between dsDNA and H-GN, a portion of H-GN will be released from the surface of Fe 3 O 4 @Au NPs and transferred into solution. After magnetic separation was performed, the supernatant was incubated with 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The released H-GN can catalyze the oxidation reaction of TMB and turn the colorless solution blue. This “signal-on” colorimetric biosensor shows a broad linear range of 0.5–100 nM for the target NOS, with a 0.19 nM detection limit. The application of the biosensor for determination of NOS segments in samples of GM and non-GM tomatoes shows that it can discriminate between GM and non-GM plants. The reliability of the method for samples of NOS-spiked GM tomato suggests satisfactory recoveries in the range of 93.6%–94.2%.
研究领域:
生物化学
·
纳米技术
·
农业科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 16
·2197-2203
·
IF 2.378
Xiaoqiang Wang,Haiyan Sun,Chengkun Liu,Chao Wang
Following an inspiration from the chemistry of protein nanocages in nature, we evaluated the performance of a hemin-functionalized GroEL nanocage as peroxidase mimics for chromogenic detection. The reactivity of this biohybrid formed by detergent dialysis was found to be highly dependent on the amount of hemin encapsulated inside the GroEL central cavity. In comparison with horseradish peroxidase (HRP), the hemin–GroEL complex exhibited a higher recognition ability and reactivity towards the chromogenic substrate tested, while, similar to HRP, the peroxidase mimic followed a ping-pong catalytic mechanism. Moreover, the efficacy of hemin–GroEL for the chromogenic detection of H2O2 or glucose was demonstrated, with the detection limits determined to be 7 μM for H2O2 and 11 μM for glucose. This assay was also shown to be useful for the oxidative removal of phenolic compounds. Thus, the GroEL cage is a valuable tool for the development of analytical nano-materials and practical applications such as clinical analysis and environmental monitoring.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
环境科学
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DOI
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 31
·13231-13240
·
IF 7.79
Qing Wang,Naijie Wei,Jingru Guo,Kai Feng,Yin Kwan Wong,Jingwei Zhang,Jigang Wang,Xiaolian Sun
Although artemisinin (ART) has shown initial promise in cancer therapy, its therapeutic efficacy is limited by its low tumor inhibitory efficacy and unfavorable distribution. Considering the important role of heme in the specific parasite-killing effect of ART, we designed a liposomal nanostructure self-assembled from hemin-lipid (Hemesome) to co-deliver ART and hemin for cancer therapy. The synergistic chemotherapeutic and immunotherapeutic effects of hemin and ART were demonstrated both in vitro and in vivo. The liposome-like structure was relatively stable in the blood circulation and gastrointestinal tract environment, but dissociated in the tumor cell environment. The folic acid (FA) modification not only increased their efficiency for transport across the epithelium, but also increased their tumor accumulation. In mouse models, following oral administration of FA-Hemesome-ART nanoparticles (5 mg kg−1 ART in total) every other day and intraperitoneal injection with a programmed death-ligand 1 antibody (aPD-L1, 70 μg per mouse in total), MC38 tumors were completely inhibited within 30 days. The cured mice remained tumor-free 30 days after rechallenging them with another inoculation of MC38 cells due to the strong immune memory effect.
研究领域:
药学
·
肿瘤学
·
纳米技术
·
免疫学
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DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 12
·3409-3415
·
IF 3.969
Xiaomeng Meng,Yunlei Zhou,Qianjin Liang,Xiangjin Qu,Qingqing Yang,Huanshun Yin,Shiyun Ai
A simple and novel microRNA (miRNA) biosensor was developed using DNA-Au bio bar code (DNA-Au) and G-quadruplex-based DNAenzyme. DNA-Au increased the amount of miRNA-21 participating in hybridization. Hemin/G-quadruplex DNAenzyme significantly improved the catalysis of H2O2 by oxidation of hydroquinone, resulting in an obvious reduction current of benzoquinone for miRNA-21 indirect detection. Under optimum conditions, the linear relationship between miRNA-21 concentration and reduction response was obtained with the detection limit of 0.006 pM, which showed a good sensitivity. Besides, selectivity of the biosensor was investigated by detecting the base mismatched miRNAs. This proposed method was further applied to detect miRNA-21 extracted from human hepatocarcinoma BEL-7402 cells and human mastocarcinoma MCF-7 cells. The influence of bisphenol A (BPA) on the expression of miRNA-21 in cells was also investigated. The biosensor performs well in practical applications, which suggests it may provide a new platform for gene diagnosis.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
癌症研究
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DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 6
·1343
·
IF 4.6
Yiaobo Zhang,Lin Liu,Jiahui He,Chengzhi Huang,Lei Zhan,Chunmei Li
The development of sensitive and specific methods for the high-quality analysis of hemin-related drugs is significant in the pharmaceutical field. In this work, a simple and rapid method based on the fluorescent properties of carbon dots (CDs) was established for the determination of hemin in drugs. By taking melamine and ethylenediamine as the reaction materials, the fluorescent CDs were synthesized by a one-step solvothermal method, which can be used for the determination of hemin in drugs by the fluorescent inner filter effect. The as-prepared fluorescent CDs with rich functional groups on the surface displayed good water solubility, strong salt resistance, robust pH stability, and photobleaching resistance. Most importantly, the fluorescent excitation wavelength of fluorescent CDs was very close to the absorption wavelength of hemin, providing the evidence for the fluorescent inner filter effect. When the hemin concentration was in the range of 0.01–1 μM, there was a good linear relationship between the hemin content with the fluorescence intensity of CDs. The linear regression equation was (1 −F/F0) = 0.0897c+ 0.0124, with a correlation coefficient (R2) of 0.9982 and a detection limit of 9 nM. This assay was successfully used to determine the content of hemin in the tablet, which displayed 97.9–105.5% of the labelled amount, with a relative standard deviation of less than 3%. The developed fluorescence method for the detection of hemin content displays the advantages of accurate, rapid, and high sensitivity, which could prove to be a useful tool for the determination of hemin supplement tablets.
研究领域:
药学
·
分析化学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 6
·1639-1649
·
IF 4.157
Fereja Shemsu Ligani,Fang Zhongying,Li Ping,Guo Jinhan,Fereja Tadesse Haile,Chen Wei
Balanced level of hemin in the body is fundamentally important for normal human organ function. Therefore, environmentally benign, stable, and fluorescent metal nanoclusters (NCs) for selective and sensitive detection of hemin have been investigated and reported. Herein, highly orange red emissive gold NCs are successfully synthesized using glutathione as a reducing and stabilizing agent (GSH-Au NCs). The clusters are characterized using various techniques like Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), UV-vis spectroscopy, and fluorescence spectrometer. The fluorescence intensity of as-synthesized Au NCs strongly quenched upon addition of different concentrations of hemin. The decrease in fluorescence intensity of GSH-Au NCs has been applied for determination of hemin concentration in the linear range from 1 to 25 nM with a low limit of detection (LOD) of 0.43 nM. The method was also successfully applied for quantification of hemin in human serum sample. In view of this reality, the system can be considered as a possible strategy and excellent platform for determination of hemin in various areas of application. Graphical abstract
研究领域:
生物化学
·
医学检测
·
纳米技术
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）