JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2016
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IF 2.687
Xiao-Mei He,Bi-Feng Yuan,Yu-Qi Feng
Immobilized metal affinity chromatography (IMAC) technique is frequently used in the purification of histidine-tagged (His-tagged) recombinant proteins. In this study, nickel(II)-immobilized carboxyl cotton chelator (CCC-Ni 2+ ) fibers was synthesized by a simple method based on the coordination effect between Ni 2+ and carboxyl group. The nickel content of the CCC-Ni 2+ fibers was determined to be 5 times larger than that of Ni 2+ -immobilized sulfhydryl cotton fiber (SCF-Ni 2+ ) fibers developed in our previous work. The prepared CCC-Ni 2+ fibers were then applied for the selective and rapid separation of His-tagged protein from escherichia coli ( E. coli ) cell lysates on the basis of the high affinity of Ni 2+ to 6 × His with a lab-in-syringe format. Benefiting from the good biological compatibility and high nickel content, the results showed that CCC-Ni 2+ fibers were able to selectively capture His-tagged proteins from complex E. coli cell lysates and exhibited a relatively large adsorption capacity toward His-tagged protein. The recoveries of His-tagged GFP in E. coli cell lysates were in the range of 89.8%-106.7% with the relative standard deviations (RSDs) less than 9.4% (intra-day) and 10.3% (inter-day). Taken together, this efficient approach for the purification of recombinant proteins extends the application of CCC-based fibrous materials in biological analysis.
研究领域:
生物化学
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蛋白质工程
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分析化学
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高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 10
·2185-2191
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IF 4.726
Jiangnan Zheng,Zian Lin,Guo Lin,Huanghao Yang,Lan Zhang
This work reports a novel metal–organic framework (MOF)-based metal affinity platform for the rapid and highly specific separation of histidine-rich proteins using zeolitic imidazolate framework-8 coated magnetic nanocomposites (denoted as Fe3O4@ZIF-8). The coating of the ZIF-8 layer on the Fe3O4 core was performed in aqueous solution at room temperature and merely took 10 minutes. The monodisperse Fe3O4@ZIF-8 has an average diameter of 190 nm, displays superparamagnetism with a saturation magnetization value of 47.9 emu g−1, and possesses a large external surface area of 131.0 m2 g−1. Due to the high density of low-coordinated Zn atoms on the surface of ZIF-8, Fe3O4@ZIF-8 exhibited a large adsorption capacity for model histidine-rich proteins (>6000 mg g−1 for bovine hemoglobin) and relatively low adsorption capacities for other proteins which contain fewer surface-exposed histidine residues. Moreover, Fe3O4@ZIF-8 showed excellent recyclability (more than 10 times) with high recovery (88.4%). In addition, Fe3O4@ZIF-8 can be used to selectively separate hemoglobin from a protein mixture and human blood samples. The good results demonstrate the potential of Fe3O4@ZIF-8 in the separation of histidine-rich proteins.
研究领域:
生物化学
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材料科学
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高影响因子
ELECTROCHIMICA ACTA
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2021
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IF 6.901
Xiaofeng Wei,Ying Chen,Shan He,Huiting Lian,Xuegong Cao,Bin Liu
Metal-organic framework (MOF)-based electrochemical interface presents a broad platform for the chiral recognition of amino acids , but the inferior conductivity of most MOFs limits the sensitivity in practical applications of electrochemical sensing. Therefore, the L-histidine-regulated zeolitic imidazolate framework (L-His-ZIF) was proposed as a novel electrochemical interface for simple, efficient, and enantioselective determination of L-glutamate (L-Glu) based on the electrochemical impedance spectroscopy (EIS). Based on the resistance change, the L-His-ZIF interface can be directly used for chiral recognition without the assistance of high-conductive substances, where multiple immobilizations are generally required in MOF-involved voltammetry . The stacked L-His-ZIF nano-polyhedrons provided numerous cavities and high specific surface area, thereby forming adequate chiral recognition sites. Based on the L-His-ZIF interface, the enantioselective detection of l -Glu-was successfully achieved with a good linear relationship with the impedance difference in the range from 0.1 nM to 50 nM with the detection limit of 0.06 nM. This prepared ZIF-functionalized electrode provided the desired enantioselectivity and reproducibility with robust chiral recognition in the real application. More importantly, MOF-modified EIS can be applied for more chiral recognition of enantiomers by various MOF interfaces, which has great potential for applications in pharmaceutical monitoring and biochemical analysis.
研究领域:
分析化学
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生物医学工程
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Meng Sha,Weiqing Xu,Yu Wu,Lei Jiao,Yifeng Chen,Jiajia Huang,Yinjun Tang,Wenling Gu,Chengzhou Zhu
The elaborate design and fabrication of nanomaterials to mimic natural enzyme-catalyzed active sites is a promising approach to significantly improve catalytic performances, which is beneficial to the signal amplification for biosensing applications. Metal–organic frameworks (MOFs) with tailorable components, structures and well-distributed metal sites are supposed to tackle the challenges in traditional nanomaterials of vague structure and imprecise coordination. Herein, defect-engineered MOFs with histidine-functionalized active sites (His-MIL-101) has been constructed by the missing linker strategy. Bearing a histidine unit in the iron active site, the resultant His-MIL-101 can vividly mimic the natural enzyme catalytic pocket and exhibits double the peroxidase-like activity of parent MOFs. Notably, the introduction of histidine can not only increase the specific surface area for boosting the metal active site exposure but also provide an optimized electronic structure of active sites to promote the generation of active intermediate (hydroxyl radical). By virtue of the synergistic effects of metallothioneins on metal active site-blocking impact and free radicals scavenging, His-MIL-101-based colorimetric biosensing platform was constructed to accurately detect metallothioneins with a wide detection range from 20 nM to 50 μM, as well as a low detection limit of 10.49 nM. This approach paves the way for the design of advanced biomimetic nanomaterials to efficiently mimic the active center of natural enzymes and further extend the application in biosensing.
研究领域:
纳米技术
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生物传感器
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材料科学
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分析化学
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高影响因子
ANALYTICAL LETTERS
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2021
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IF 2.329
Zhipeng Su,Fanyan Ye,Kaiyu He,Tao Yang,Wang Li,Jiali Ren
The sensitive determination of pesticides constitutes an important aspect in ensuring food safety. Due to the disadvantages of traditional methods, such as low sensitivity, complex operation, and high cost, the development of an efficient and economical biosensor to detect pesticide residues is required. In this work, the dipeptide glycyl-L-histidine (GH) and divalent copper ions (Cu2+) were employed as bioorganic linkers and metal ions in the synthesis of the peptide-based metal organic framework (MOF) material GH-Cu BioMOF. Because of its properties of fluorescence quenching and adsorption of nucleic acids, GH-Cu BioMOF was used to develop a distinctive aptamer based method for the determination of pesticide residues in food. The GH-Cu BioMOF-based aptasensor was used for acetamiprid and demonstrated a linear response in the range from 0.15 to 3.00 µM with a detection limit of 137 nM, verifying the feasibility of this method for pesticide determination in food.
研究领域:
食品安全
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分析化学
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生物传感器
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Conghua Yi,Qinghe Xu,Dongjie Yang,Miao Wang
In this work, we developed a novel technique to prepare pH-responsive charge reversal nanospheres based on softwood kraft lignin (SKL) through two steps modification and high efficient self-assembly. SKL was first grafted with imidazole groups by Mannich reaction and further fabricated into acetylated histidine-modified lignin (Ace-SKL-HIS) via acetylation reaction. Ace-SKL-HIS nanospheres (Ace-SKL-HIS NPs) were prepared by fast vacuum evaporation method, their effective diameters (ED) were around 100 nm, exhibited low Polydispersity Index(PDI, <0.2) and good shape. Ace-SKL-HIS NPs demostrated excellent pH response performance with charge reversal under acidic environment. Then, curcumin was applied as a model drug to prepare drug-loaded nanospheres ( [email protected] ). The drug loading degree (DL%) and encapsulation efficiency (EE%) were 22.34 % and 70.76 %, respectively. The in vitro release behavior of curcumin exhibited excellent pH dependent release property, 76.82 % release at pH 5.7 while only 12.92 % at pH 7.4 in 120 h. Furthermore, in vitro cytotoxicity studies showed that [email protected] had potent anti-tumor effect, with IC 50 of 28.16 μg/ mL. This study constructed a promising nanocarrier platform for cancer drug delivery, which paved a new way to the high value-added application of lignin.
研究领域:
纳米技术
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药学
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生物医学工程
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材料科学
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高影响因子
MOLECULES
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2024
·Vol. 29
·期 10
·2167
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IF 4.2
Yuxia Li,Min Li,Liuzhi Hu,Baozhu Zhang
In this study, Cu2+modulated silver nanoclusters were constructed for the turn-on, label-free detection of L-histidine. Six Ag NCs protected by oligonucleotides (DNA-Ag NCs) were tested in a series of experiments. Finally, A-DAN-Ag NCs were chosen as the best candidate due to their excellent fluorescent properties. The fluorescence of A-DAN-Ag NCs was quenched using Cu2+through energy or electron transfer. However, quenched fluorescence could be restored dramatically in the presence of L-histidine due to Cu2+liberation from A-DAN-Ag NCs and because of the chelation between the imidazole group of L-histidine and Cu2+. The proposed sensor exhibited high selectivity towards L-histidine over other amino acids, with a limit of detection (LOD) of 0.096 μM ranging from 0 to 8 μM. The proposed sensor succeeded in detecting L-histidine in diluted human urine. Therefore, the sensor has promising practical applications in biological systems.
研究领域:
生物化学
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分析化学
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生物医学
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高影响因子
LUMINESCENCE
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2024
·Vol. 39
·期 1
·e4677
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IF 3.2
Shen Zhang,Jinlong Ma,Yangfan Wu,Jingwen Lu,Yuyu Guo
There is a significant need to accurately measure doxycycline concentrations in view of the adverse effects of an overdose on human health. A fluorescence (FL) detection method was adopted and copper nanoclusters (CuNCs) were synthesized using chemical reduction technology. Based on FL quenching with doxycycline, the prepared CuNCs were used to explore a fluorescent nanoprobe for doxycycline detection. In an optimal sensing environment, this FL nanosensor was sensitive and selective in doxycycline sensing and displayed a linear relationship in the range 0.5–200 μM with a detection limit of 0.092 μΜ. A characterization test demonstrated that CuNCs offered active functional groups for identifying doxycycline using electrostatic interaction and hydrogen bonds. Static quenching and the inner filter effect (IFE) resulted in weakness in the FL of His@CuNCs with doxycycline with great efficiency. This suggested nanosensor was revealed to be a functional model for simple and rapid detection of doxycycline in real samples with very pleasing accuracy.
研究领域:
分析化学
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医学检测
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纳米技术
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PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2026
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IF 4.8
Bo Zhang,Xintian Liu,Tianjun Zhao,Jinkang Hao,Zejian Wang,Menglei Xia,Ning Chen,Xiaonan Wang,Xiaoguang Fan
l -Histidine is an essential amino acid with important applications in pharmaceuticals and nutrition, highlighting the demand for efficient microbial production platforms. This study developed a high-performance Escherichia coli cell factory through systematic metabolic engineering. First, we mined a feedback-resistant hisG∗ smar and the entire mutant his operon from a previously obtained high l -histidine-producing mutant of Serratia marcescens , offering novel enzymatic parts beyond conventional sources. Combined with precursor supply enhancement and redox balancing, the engineered strain yielded 4.46 g/L l -histidine. Second, a machine learning-based platform (TransDW) was utilized to predict and validate a novel efflux transporter, Cgl1374, increasing titer to 4.82 g/L. Third, we implemented a growth phase-dependent system to dynamically regulate pgi expression, redirecting carbon flux and achieving 5.49 g/L in shake flasks. Finally, applying a novel carbon evolution rate (CER)-based control strategy in fed-batch fermentation, the optimized strain achieved 49.8 g/L of l -histidine in a 5-L bioreactor, with a yield of 0.265 g/g glucose, which is the highest yield reported for engineered E. coli . This work establishes a synergistic framework combining non-model gene discovery, computational transport engineering, and real-time physiological feedback control, providing a versatile blueprint for next-generation microbial cell factories.
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高影响因子
International Journal of Electrochemical Science
·
2023
·Vol. 11
·期 11
·9175-9191
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IF 1.5
Zhe Zhang,Xiaodong Huang,Ningchen Tian,Fusong Ni,Le Ruan,Yuzeng Lv,Ling Wu
The histidine and its derivatives self-assembled (SA) films were prepared on the 304 stainless steel (SS) surface. EIS and potentiodynamic polarization curves measurements proved that these SA films could effectively protect SS against corrosion in 3.5% NaCl solution, with a maximal protection efficiency of 94.24% achieved by Carbobenzyloxy-L-histidine film. XPS studies confirmed that Carbobenzyloxy-L-histidine molecule could adsorb on SS surface. The quantum chemical calculation and dynamic simulation provided the theoretical support about the inhibition mechanism.
研究领域:
材料科学
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腐蚀工程
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表面化学
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电化学
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
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IF 9.1
Wei Yang,Ping Xiong,Yinghong Wang,Tao Tao,Xuerui Liu,Xiaoling Xu,Caiyun Yang,Liming Yang,Huiji Xiao,Kai Zhang,Wei Zhang,Xubiao Luo
Membrane separation technology serves as an essential approach for the efficient and sustainable extraction of lithium from salt-lake brines, whose effectiveness crucially depends on the selective Li + /Mg 2+ separation performance. However, conventional nanofiltration (NF) membranes remain constrained by the trade-off between water permeability and ion selectivity. In this study, we proposed a two-step fabrication strategy that couples solution deposition with interfacial polymerization (IP), successfully constructing a positively charged Zr-His/PTF composite NF membrane with a thin selective layer and uniform pore size. Hydrated zirconia clusters (hydrous-ZrO 2 ) derived from inorganic zirconium salts were utilized to regulate the distribution of reaction sites via electrostatic anchoring, while the amphipathic structure of histidine was employed to restrict the diffusion of aqueous monomers. These two components synergistically modulated the IP process, enabling the precise regulation of the selective layer microstructure while significantly reducing organic reagent consumption. Under a high Mg 2+ /Li + mass ratio of 30:1, the modified membrane achieved a synchronous enhancement in Li + /Mg 2+ separation selectivity (29.57) and water permeance (21.7 L·m −2 ·h −1 , 6 bar). Moreover, the membrane maintained stable separation performance and structural integrity across a wide Mg 2+ /Li + mass ratio range (1:1 to 100:1) and throughout 100 h of continuous operation. Through the integration of fabrication methods, this study provides a straightforward, low-cost and environmentally friendly strategy for fabricating high-performance NF membranes.
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高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·10075
·
IF 15.7
Jin Hao,Zhu Xuanqi,Zhang Mengsi,Zheng Hao,Chen Yang,Yao Dong,Liu Shuwei,Zhang Hao
Superoxide dismutase (SOD), featured with unique metal catalytic sites, is the first defense against reactive oxygen species (ROS) damage in vivo and well used in the treatment of oxidative stress-related diseases. However, the catalytic activity of natural SOD is limited by the metal ion coordination with rigid amino-acid residues. Inspired by the Cu 2+ /histidine residue coordination in Cu-Zn-SOD, in this work, the Gibbs free energy change of the break/reform of coordination bonds in the tetracoordinated structure formed by Cu 2+ and different groups in histidine is calculated, which reveals that in comparison to the Cu catalytic site coordinated with four imidazolyls, the coordination with one amino, one carboxyl and two imidazolyls possesses the lowest overall energy as well as the lowest breaking energy of one of the coordination bonds. Thus, catalytic site stability and catalytic reaction activity can be balanced sufficiently. Accordingly, the as-prepared Cu/Zn/histidine (CuZnHis) supramolecular assemblies with optimized Cu catalytic site present a significantly enhanced SOD activity up to 37900 Unit/mg, which is at least 5.4 times higher than that of natural Cu-Zn-SOD. As a proof of concept in treating oxidative stress-related diseases, the CuZnHis assemblies promote macrophage polarization from M1 to M2 phenotype and the expression of anti-inflammatory factors, and inhibit the periodontitis in male animal models.
研究领域:
生物无机化学
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超分子化学
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酶模拟
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氧化应激治疗
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纳米医学
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高影响因子
危险品化学品经营许可证（带存储）