ANALYTICAL LETTERS
·
2023
·
IF 2
Yimeng Zhao,Xuefei Lv,Zhao Peng,Kexin Zhao,Di Zhou,Yulin Deng
Abnormally expressed miRNAs have been employed as crucial indicators for the diagnosis and prognosis of disease. It is essential to develop quick, affordable, accurate, and multiplexed methods for miRNA detection. In this study, polyethylene glycol (PEG) hydrogel microparticles were used as solid-phase carriers for the hybridization chain reaction (HCR) in combination with cation-exchange fluorescence amplification, thus enabling the development of a new method for miRNA determination. The assay was sensitive with the detection limit for miRNA let7a equal to 46 pM. The assay was specific, the base-mismatched sequences of miRNA let7a were distinguished, and the serum matrix did not cause any interference. Moreover, by integration with a microfluidic chip, the throughput was significantly enhanced and the process was noticeably simplified. With further development, the developed method may be suitable for practical clinical analysis.
研究领域:
生物医学工程
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分子诊断
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分析化学
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产品关联
DOI
高影响因子
PHARMACOLOGY
·
2024
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IF 2.9
Zhong Wenyan,Chen Jingjing,Xu Guangfu,Xiao Li
Introduction: To investigate the effect and mechanism of Kaempferol on alcoholic steatohepatitis. Methods: C57BL/6 N mice were utilized to establish Binge-on-Chronic alcohol exposure mice model. Kaempferol was given as the interventional drug to chronic alcohol-fed mice for six weeks to assess its effects. In vitro, intestinal epithelial Caco-2 cells were stimulated by alcohol, and miRNA-155 mimics were used to further study the effect of kaempferol to miRNA-155 signaling in intestinal epithelial cells. HE staining and oil red O staining were used to observe the liver and intestinal tissue damage in each group of mice, and ALT, AST, IL-1B and TNF-a were detected by kits; LPS expression was detected by ELISA kit, and the expression of IL-1B and TNF-a was assessed by qRT-PCR; activated inflammatory response of liver and colon tissue and the related signalling pathway activation. Results: Kaempferol treatment significantly improved pathological changes such as steatosis and vacuolated lesions in liver tissue of the alcohol diet model group, and reduced serum ALT and AST enzyme activities and liver tissue interleukin-1β and tumour necrosis factor-α mRNA expression levels. Kaempferol significantly reduced the expression of miRNA-155 in the intestinal tissue of alcohol-fed mice, significantly increased their SOCS1 protein expression, inhibited the activation of nuclear factor kappa-B and significantly increased the production of the intestinal tight junction proteins occludin and ZO-1. What's more, kaempferol significantly reduced serum LPS levels in ASH mice. In vitro experiments showed that compared with the control group, kaempferol significantly inhibited the expression level of miRNA-155 in Caco-2 cells under ethanol exposure, decreased the activation of nuclear factor kappa-B, led to an increase in the expression of SOCS1 protein, and increased the production level of occludin protein in Caco-2 cells under the effect of alcohol. In contrast, overexpression of miRNA-155 significantly decreased occludin and SOCS1 protein production and increased nuclear factor kappa-B activation levels in Caco-2 cells, and the administration of kaempferol significantly inhibited this effect. Conclusion: Kaempferol improved the stability of gut barrier function to ameliorate hepatic injury induced by alcohol intake through enhancing occludin protein expression, by targeting miR-155 to inhibit the excessive inflammatory response in the intestine
研究领域:
药理学
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肝病学
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胃肠病学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 16
·167502
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IF 3.721
Yanjie Zheng,Yichun Xu,Li Chen,Xinyang Yin,Fei Lin,Shaohuang Weng,Xinhua Lin
In this study, a novel dual-modal electrochemical impedance spectroscopy (EIS) and electrochemiluminescence (ECL) biosensor for sensitive detection of femtomolar miRNA-126 was developed based on hybridization chain reaction (HCR). The capture unit was Fe3O4@SiO2@AuNPs-cDNA, being Fe3O4@SiO2@AuNPs nanoparticles coated by hairpin cDNA which could capture miRNA-126 specifically. The signal unit was HCR-Ru(phen)32+, which was a long double-stranded DNA obtained through HCR with a great number of ECL signal labels Ru(phen)32+ embedded. In presence of target miRNA-126, stem-loop structure of cDNA in the capture unit was opened and a partial dsDNA was formed, the residue bases of which hybridized with the signal unit to form a capture unit/miRNA-126/signal unit complex on the electrode surface. In this case, dual-modal biosensor was prepared easily by the help of magnet, and EIS and ECL detection was both acquired. In addition, a miRNA-126 molecule corresponded to a long double-stranded DNA and a large amount of Ru(phen)32+ ions embedded, so the electrochemical impedance and the ECL intensity were greatly increased, with a limit of detection (LOD) of 2 fM. And, EIS and ECL results could be checked mutually, improving the detection accuracy and reliability. It offers a simple, fast, sensitive, selective and accurate approach for versatile analysis of microRNAs.
研究领域:
生物传感器技术
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分子诊断学
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纳米医学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Wei Xie,Shu Jia,Rongjun Yu,Jianjiang Xue,Shulian Bai,Chenxing Wang,Gang Tian,Qiongyuan Zhang,Xiao Gui,Lilin Wang,Silin Chen,Ke Lin,Wei Mao,Yu Huang,Jiangling Wu,Gang Liu
Sepsis represents a life-threatening systemic inflammatory condition characterized by organ dysfunction, where the identification of specific biomarkers and development of effective diagnostic modalities are critical for timely clinical intervention to mitigate disease progression. Emerging evidence positions exosomal microRNAs (exomiRs) as promising novel biomarkers with multi-dimensional clinical utility spanning early diagnosis, therapeutic efficacy assessment, and longitudinal prognostic monitoring in sepsis management. However, the direct rapid detection of exomiRs in clinical specimens undergoes severe critical challenges due to the absence of robust detection methodologies with these characteristics of operational simplicity, high sensitivity and analytical robustness. Here, we designed and constructed a hairpin/loop-constrained three-dimensional topological self-assembling nanostructure, which was denoted as DNA molecular lock (DML), and developed a spatially allosteric-mediated electrochemical nanobiosensor for ultrasensitive and specific analysis of exomiR-125b. This independent, rapid, and portable detection method, combined with Ti 3 C 2 T x MXene/AuAgNPs nanozymes, achieved excellent sensitivity with a linear range from 2 fM to 200 nM and a low detection limit of 0.15 fM. It provided required low-volume plasma samples (2 mL) for analysis while supported Bluetooth-enabled wireless communication and real-time data processing. Furthermore, integration of machine learning algorithms with this developed nanobiosensor improved differential diagnostic accuracy to 96.6 % in the training cohort ( n = 83) and 89.2 % in the validation cohort ( n = 57), effectively distinguishing sepsis patients (SPs), general inflammation patients (GIPs), and healthy donors (HDs). Our study establishes the sensor's efficacy in improving sepsis detection through biomarker-guided analysis. These findings highlight the potential application of this developed nanobiosensors for portable, personalized bedside monitoring of exomiRs as the point-of-care testing (POCT) platforms.
研究领域:
生物传感器
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分子诊断学
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纳米技术
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微小RNA研究
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临床检验医学
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医疗健康中的机器学习
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产品关联
DOI
高影响因子
Analytical Methods
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2025
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IF 2.6
Yunliang Liu,Jing Gao,Yuting Huo,Yang Yang,Daoping Yue,Xianghang Lin
MiRNA-155 is a reliable biomarker for cancers and allergic rhinitis, also plays a critical role in regulating gene expression and mediating the allergic TH2 process. The accurate determination of miRNA-155 will be significant for diagnosing allergic rhinitis, especially for children. Herein, Yb-Bi2S3 nanorods were prepared through a hydrothermal method. Gold nanoparticles (AuNPs) were assembled onto Yb-Bi2S3 nanorods surface to provide a Yb-Bi2S3/AuNPs nanocomposite. Yb-Bi2S3/AuNPs nanocomposite was used as a photoactive element for fabricating a near-infrared photoelectrochemical aptasensing platform by assembling the aptamer for specific recognition of miRNA-155. The photocurrent variation of the photoelectrochemical aptasensor before and after recognizing miRNA-155 is linearly related to the miRNA-155 concentration in the range of 1.0 fmo l L-1 ~ 1.0 nmo l L-1. The detection limit was calculated to be 0.34 fmol L-1. The developed photoelectrochemical aptasensor was successfully employed for determining miRNA-155 in serum samples with satisfactory results, indicating possessing practical applications in the determination of miRNA-155 in real samples.
研究领域:
生物医学工程
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分子诊断学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Rong Wang,Junlong Wang,Xin Zhang,Siyu Liao,Yusi Zhou,Qincong Feng,Youjiang Tan,Yuehua Huang,Yuanyuan Dai,Yuanwen Jiang,Haimei Qin
Breast cancer (BC) is the most common malignancy in women and ranks second among all cancer types worldwide. The escalating demand for early and precise diagnosis has intensified the quest for ultrasensitive and highly specific biosensors. Here we present a self-referencing photoelectrochemical (PEC) strategy based on a nanopipette that enables the direct quantification of the BC biomarker miRNA-21 in single living cells. The nanopipette's ultrafine tip minimizes membrane disruption, while an integrated self-referencing electrode cancels out trans-membrane potential fluctuations, yielding a linear dynamic range of 1–7 nM and a detection limit of 0.328 nM without cell lysis or amplification. The platform markedly enhances the accuracy of single-cell heterogeneity analyses and offers a new paradigm for intracellular trace-nucleic-acid quantification. Moreover, the modular self-referencing architecture creates a methodological foundation for extending nanopipette-based sensors to broader single-cell omics applications.
研究领域:
生物化学
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生物传感器
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癌症研究
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纳米技术
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分析化学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Materials Horizons
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2025
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IF 10.7
Linlin Zhao,Meiyu Hu,Xiaohang Yin,Hao Yang,Liyun Zhu,Pingyuan Sun,Xiya Wang,Songwei Ai,Yonghui Zheng,Genjie Li,Tingting Yang,Xuerui Chen,Jingyu Zhang
Effectively alleviating cerebral ischemia-reperfusion (I/R) injury is challenging despite medical advances. Prompt restoration of blood flow after ischemic stroke causes secondary damage to brain tissue, triggers neuroinflammation and overproduces reactive oxygen species (ROS). MiRNA regulates genes involved in neuron apoptosis and neuroinflammation, thus exhibiting potential in ameliorating cerebral I/R injury. However, as miRNA is vulnerable to degradation, its effective delivery poses obstacles to clinical applications. Inspired by therapeutic potential of miR-210 inhibitors in ischemic stroke-induced neuroinflammation, we constructed a multifunctional nanosystem composed of ceria nanozymes and zeolitic imidazolate framework-90 (ZIF-90) nanoparticles to deliver miR-210 inhibitors to treat cerebral I/R injury. Attributed to proton sponge effect of ZIF-90, this nanosystem allows for lysosome escape of miR-210 inhibitors to protect their intracellular bioactivity, while integration of ZIF-90 and ceria nanozymes utilizes multi-enzyme cascade activities to constrain lipid peroxidation and reduce oxidative damage in brain tissues of mice with middle cerebral artery occlusion (MCAO). After crossing the blood-brain barrier, miR-210 inhibitors target TET2 to suppress pro-inflammatory cytokines, finally inhibiting neuroinflammation. More than uncertain stability and efficacy of direct TET2 protein administration, delivery of miR-210 inhibitors by multifunctional nanosystems engendered neuroprotection, indicating their potential for protein replacement therapy against cerebral I/R injury.
研究领域:
神经科学
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纳米技术
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分子生物学
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药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Dose-Response
·
2021
·Vol. 19
·期 1
·15593258211001255-15593258211001255
·
IF 2.658
Chen-Xia Wen,Hai-Li Tian,E Chen,Jin-Fang Liu,Xiao-Xing Liu
Objective Our group aimed to investigate the expression pattern of miRNA-873-5p in cervical cancer (CC) patients and its association with CC progression. Methods Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied for the examination of the expressions of miRNA-873-5p in both CC specimens and cell lines. The clinical significance of miRNA-873-5p was statistically analyzed. MTT, colony formation, Transwell and flow cytometry assays were used to detect cell proliferation, metastasis, and apoptosis changes of Hela and Siha cell line. Luciferase reporter assays and Western blots were utilized to identify the target genes of miRNA-873-5p. Western blot and RT-PCR were used to judge the dysregulation of Notch signaling. Results Our results indicated that miRNA-873-5p expression was distinctly reduced in CC patients. Low miRNA-873-5p expressions were distinctly correlated with positively distant metastasis, The International Federation of Gynecology and Obstetrics (FIGO) stage and poor prognosis of CC patients. A functional assay using miRNA-873-5p mimics indicated that overexpression of miRNA-873-5p distinctly suppressed CC cells proliferation and metastasis, and promoted apoptosis. Bioinformatic assays revealed that miRNA-873-5p may target the 3'-UTR of ZEB1 and lead to the suppression of its translation, which was verified by the use of luciferase assays. Finally, overexpression of miRNA-873-5p suppressed the expressions of Jag1, Maml2 and Hey1. Conclusion Taken together, we firstly provided evidence that miRNA-873-5p expression was a poor favorable factor for CC patients, and the use of miRNA-873-5p may represent a and potential biomarker and promising therapeutic approach for CC.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 9
·724
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IF 5.743
Yang Qing,Yuxing Yang,Ping Ouyang,Chenxin Fang,Haobin Fang,Yazhen Liao,Haiyu Li,Zhencui Wang,Jie Du
Because microRNAs (miRNAs) are biological indicators for the diagnosis, treatment, and monitoring of tumors, cancers, and other diseases, it is significant to develop a rapid, sensitive, and reliable miRNA detection platform. In this study, based on miRNA-21 detection, DNA-a with a 3′ end overhang and Texas Red fluorophore-labeled 5′ end was designed, which reacts with miRNA-21 and hybridizes with exonuclease III (Exo III), where the part connected to miRNA-21 is hydrolyzed, leaving a-DNA. At the same time, miRNA-21 is released to participate in the following reaction, to achieve cyclic amplification. a-DNA reacts with DNA-b conjugated to gold nanoparticles to achieve fluorescence quenching, with the quenching value denoted asF; additionally, after adding DNA-d and linked streptavidin immunomagnetic beads (SIBs), fluorescence recovery was achieved using DNA-c, with the recovered fluorescence recorded asF0. By comparing the difference in the fluorescence (F0−F) between the two experiments, the amount of DNA-a hydrolyzed to produce a-DNA was established to determine the target miRNA-21 content. Under optimized conditions, by comparing the changes in the fluorescence signal, the developed strategy shows good sensitivity and repeatability, with a detection limit of 18 pM, good discriminative ability and selectivity, and promise for the early diagnosis of breast and intestinal cancers.Keywords:exonuclease III;miRNA-21;AuNPs;fluorescence quenching;fluorescence recovery
研究领域:
生物化学
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分子生物学
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癌症研究
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Haiyan Ma,Ning Xue,Zongbing Li,Ke Xing,Xiangmin Miao
Multi-walled carbon nanotube-gold nanocomposites (MWCNT/AuNCs) were prepared and used as a novel fluorescence quenching platform for ultrasensitive detection of miRNA-155. In the designed system, fluorophore labeled DNA probe was introduced, and the fluorescence signal of them were effectively quenched due to the adsorption of them on MWCNT/AuNCs. Upon the addition of target miRNA-155, DNA-RNA heteroduplexes were formed and desorbed from MWCNT/AuNCs to yield a strong fluorescence signal. In the presence of duplex-specific nuclease (DSN), DNA-RNA heteroduplexes became a substrate for the enzymatic hydrolysis of DNA strands and triggered cyclic signal amplification. Under optimal conditions, MWCNT/AuNCs displayed 93.2% of the quenching efficiency to the fluorophore labeled DNA probe, paving the way for ultrasensitive miRNA-155 detection with a detection limit down to 33.4 fM. Meantime, the present strategy showed excellent selectivity for miRNA-155 detection against other miRNAs including miRNA-21, miRNA-141, and mutated miRNA-155 strands. Moreover, satisfactory results were obtained for miRNA-155 detection in human serum samples, illustrating that the proposed sensing platform is applicable in disease diagnosis.
研究领域:
生物化学
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分子诊断
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Advances
·
2023
·
IF 4.7
Chunying Cui,Shuang Zhang,Dandan Li,Danni Liu,Yaoqi Wang,Qi Sun,Ran Sun,Jie Zhang,Xiaoxuan Hong,Ran Huo
Atherosclerosis is a chronic inflammatory disease, characterized by a lipid accumulated plaque. Anti-oxidative and anti-inflammation as well as lipid metabolism promoting therapeutic strategies have been applied for atherosclerosis treatment. However, the therapeutic effect by a single therapeutic method is limited. Combination of the two strategies could for one thing prevent lipid accumulation cause by inflammation and oxidative stress, for another thing promote lipid efflux from atherosclerotic plaque, to normalize arteries maximumly. Hence, a multifunctional liposome co-encapsulating an antioxidant and anti-inflammatory drug epigallocatechin-3-gallate(EGCG) as well as a lipid-efflux promoting gene miR-223 was established. The system (lip@EGCG/miR-223) could encapsulate miR-223 in core areas of the liposomes to provide an effective protective effect for gene drugs. Moreover, lip@EGCG/miR-223 was smaller in size (91.28±2.28 nm characterized by DLS), making it easier to target to AS lesions which had a smaller vascular endothelial spaces. After internalized into cellular efficiently, lip@EGCG/miR-223 exhibited excellent antioxidant and anti-inflammatory effects in vitro by eliminating overproduced ROS and decreasing the level of inflammatory cytokines (TNF-α, IL-1β and MCP-1), which is due to the effect of EGCG. Besides, the lipid-efflux promoting protein ABCA1 was up-regulated when treated with lip@EGCG/miR-223. By the two therapies mentioned, lip@EGCG/miR-223 could effectively inhibit the formation of foam cells, which is a main component of atherosclerotic plaques. In AS model mice, after intravenous (i.v.) administration, lip@EGCG/miR-223 was effectively accumulated in atherosclerotic plaques, and the distribution of drugs in heart and aorta to that in kidney was significantly increased when compared with free drugs (the ratio was 6.27% for free miR-223 treated group, and was increased to 66.10% for lip@EGCG/miR-223 treated group.). By decreasing inflammation level and lipid accumulation, the arterial vessels in AS were normalized with less macrophages and micro-angiogenesis when treated with lip@EGCG/miR-223. Overall, this study demonstrates lip@EGCG/miR-223 can be developed as a potential system for atherosclerosis treatment by a combined treatment of antioxidant, anti-inflammatory and lipid-efflux promoting effects, which provides a novel strategy for safe and efficient management of atherosclerosis.
研究领域:
药理学
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心血管疾病
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纳米技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-7
·
IF 6.232
Chen Nandi,Li Junyu,Feng Xianzhen,Yang Yanping,Zhu Li,Chen Xiaomeng,Liu Xuan,Li Yang,Wang Cunchuan,Xia Ligang
A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F / F 0 = 0.0031 × C miRNA-21 + 1.0382 ( R 2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice.
Label-free and self-assembled fluorescent DNA nanopompom for miRNA detection
研究领域:
生物化学
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纳米技术
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分子生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）