JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·Vol. 105
·期 2
·890-900
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IF 3.3
Zenghong Xing,Xiaoyun Fei,Shuling Chen,Deming Gong,Xing Hu,Guowen Zhang
Background The structure of proanthocyanidins (PC) contains a large number of active phenolic hydroxyl groups, which makes it have strong antioxidant capacity. This study investigated the structural and functional properties of ovalbumin (OVA) modified by its interaction with PC. Results It was found that on increasing the concentration ratio of PC to OVA from 10:1 to 40:1, the free amino and total sulfhydryl contents of OVA decreased from 470.59 ± 38.77 and 29.81 ± 0.31 nmol mg −1 to 96.61 ± 4.55 and 21.22 ± 0.78 nmol mg −1 , respectively, and the free sulfhydryl content increased from 7.65 ± 0.41 to 9.48 ± 0.58 nmol mg −1 . These results indicated that C N and C S bonds were formed and PC was covalently linked with OVA. The PC content in the OVA–PC conjugates increased from 281.93 ± 12.92 to 828.81 ± 46.09 nmol mg −1 on increasing the concentration ratio of PC to OVA from 10:1 to 40:1. The contents of α -helix and β -turn of OVA decreased, and the contents of β -sheet and random coil increased, confirmed by circular dichroism. The tertiary structure of OVA was also altered according to the results of fluorescence and ultraviolet absorption spectra. The surface hydrophobicity of OVA–PC conjugates decreased with increasing bound polyphenol content. The conjugation of OVA to PC significantly improved its emulsification and antioxidant activity and denaturation temperature. Conclusion This study may provide valuable information for improving OVA's functional properties and its PC conjugates for applications in the food industry. © 2024 Society of Chemical Industry.
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ULTRASONICS SONOCHEMISTRY
·
2024
·
IF 8.7
Boyu Chen,Lei Chen,Chen Li,Wanhuan Huang,Yanan Zhao,Chao Ai,Hui Teng
Anthocyanins (AC) are vulnerable to degradation when affected by external factors. The present study employed ultrasound-assisted glycosylation of ovalbumin (OVA) and dextran (Dex) to generate conjugate carrier for AC to improve its stability. The results showed that sonication significantly improved the progression of Maillard reaction to OVA. Compared to traditional glycosylation, ultrasound treatment showed a higher degree of grafting, a lower number of free-SH, and smaller particle size and uniform distribution. The SDS-PAGE results indicated covalent interaction. Intrinsic fluorescence (INF), Fourier transform infrared spectroscopy (FTIR), and Circular dichroism (CD) analysis results suggested that ultrasound-assisted glycosylation altered the OVA structure. The scanning electron microscope (SEM) and X-ray diffractometer (XRD) observed that the ultrasound-assisted complex had a more compact and smoother structure and protein unfolding were better. The protein solubility increased significantly after glycosylation. Thermal gravimetric analysis (TGA) and Differential scanning calorimetry (DSC) indicated that the glycosylated conjugates can significantly improve the thermal stability of AC In addition, the AC showed an improved processing and storage stability when conjugated with glycosylated carrier. The glycosylated protein-anthocyanins complex may help provide new ideas and scientific basis for the development of naturally sourced anthocyanins-relevant products in pharmaceutical and food industry applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zuyue Li,Jiaojiao Chen,Mahmoud Abou-Elsoud,Long Sheng,Dong Uk Ahn,Dewei Shu,Meiyu Liu,Xi Huang
Storage of shell eggs converts natural ovalbumin (N-OVA) into its more thermostable forms (S-OVA). This conversion may be associated with deterioration in the foaming properties of the stored shell egg. Thus, the foaming behavior of N-OVA and S-OVA, especially their performance at different pH conditions, was conducted. Compared with N-OVA, S-OVA improved foaming ability by 29.04 % at pH 3.0 and exhibited rough foam. Regarding foaming stability, the conversion of N-OVA to S-OVA had a pronounced reduction effect, with foaming stability significantly decreasing by 28.48 %–100.00 % in pH 3.0–9.0. The spectroscopic analysis revealed that the alteration in the foaming properties of OVA was fundamentally attributed to its conformational change. Thermostable conformational transition provided S-OVA with smaller particle sizes, more flexible conformations, higher surface charge, and higher surface hydrophobicity. S-OVA at pH 3.0 showed a higher surface activity, indicating superior foaming ability. Moreover, N-OVA and S-OVA formed stiff and solid-like interfaces. Notably, N-OVA exhibited higher dilatational and elastic modulus, indicating a more compact and stable adsorption layer at air-water interface. Overall, thermostable conformational transition improved the interfacial activity of OVA and enhanced its foaming ability; however, overactive proteins were detrimental to the stabilization of its interfacial films.
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FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yang Chen,Xiangzhou Yi,Zhenyu Zhang,Baomiao Ding,Zhenshun Li,Yangchao Luo
While protein-stabilized high internal phase Pickering emulsions (HIPEs) have demonstrated promising potential for various applications in food , their poor interfacial stability remains as a major challenge to overcome. In this work, tannic acid (TA) was used to increase the stability of ovalbumin (OVA)-stabilized HIPEs. The binding clusters between OVA and TA were hypothesized by the molecular docking simulation, and the interfacial properties of OVA and TA complexes (OVA-TA) were investigated by interfacial tension and three-phase contact angle. The characteristics of HIPEs under different pHs were studied by static light scattering and confocal laser scanning microscopy (CLSM), as well as rheology. The results showed that there were some specific clusters in OVA and TA which may form hydrogen bonds and hydrophobic interactions. The wettability of OVA was significantly improved by TA, resulting in greater interfacial stability at the oil-water interface. Compared with OVA-stabilized HIPEs (O-HIPEs), OVA-TA-stabilized HIPEs (OT-HIPEs) had narrower size distribution, more compact droplet structure and denser gel network to resist droplet aggregation. Especially at pH 4.0, OT-HIPEs exhibited stronger gel network and higher viscosity than at other pH values, because of smaller electrostatic repulsion between OVA and TA, and easier crosslinking on the oil-water interface. In addition, the viscosity of OT-HIPEs was increased after 24 h incubation, indicating the formation of more hydrogen bonds. Meanwhile, OT-HIPEs showed good storage and freeze-thaw stability. Our research findings from this study are expected to provide novel insight on protein-based particles stabilized HIPEs from an interfacial perspective.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Xiuge Gao,Jiahao Gong,Ying Cai,Jiacai Wang,Jia Wen,Lin Peng,Hui Ji,Shanxiang Jiang,Dawei Guo
As immune adjuvants assisting vaccines, nanoparticle delivery systems have been widely exploited. Squalene, the major ingredient of approved adjuvant MF59, has great potential in activating immune responses. In the current study, model antigen ovalbumin (OVA) was encapsulated into squalene-based nanostructured lipid carriers (NLCs), and the chitosan, a cationic polysaccharide, was used for modifying nanoparticles to develop a functionalized and cationic nanoparticle delivery system (OVA-csNLCs). Firstly, the optimal formulation of csNLCs was successfully screened out, and had hydrodynamic diameter of 235.80 ± 5.99 nm and zeta potential of 34.90 ± 6.95 mV. Then, the generated OVA-csNLCs had no significant difference in hydrodynamic diameter and exhibited lower zeta potential of 19.03 ± 0.31 mV and high encapsulation efficiency of 83.4%. Sucrose (10%, w /w) was selected as optimal lyoprotectant, exhibiting good stability of OVA-csNLCs in the form of freeze-dried powder. More importantly, the OVA-csNLCs effectively promoted OVA antigen uptake by macrophage, significantly enhanced the level of OVA-specific IgG, and induced a Th2-based immune response in vivo. Furthermore, mice immunization experiment demonstrated that OVA-csNLCs had well biocompatibility and facilitated spleen lymphocytes proliferation. Above findings indicate that chitosan modified squalene nanostructured lipid carriers show promise as antigen delivery system and an open adjuvant platform.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Xiao Dong,Jianliang Li,Yixuan Ni,Shuna Bao,Kexin Fan,Jingjing Xu,Qiao Deng,Xu Wang
Glycoproteins play a crucial role in various biological processes, including protein folding, information transmission, nerve conduction, and molecular recognition. In this study, a novel magnetic phenylboronic acid-functionalized covalent organic framework core-shell material (Fe 3 O 4 @BBCOF-BA) was successfully synthesized for the highly selective enrichment of glycoproteins from complex biological samples. Systematic optimization of experimental conditions revealed that under weakly alkaline conditions at pH 8.5, the material specifically recognized glycoproteins through boronate ester bonds. The maximum adsorption capacity for ovalbumin (OVA) reached as high as 469.45 mg/g, representing a two-to threefold improvement over traditional magnetic boronate affinity materials. Adsorption kinetics indicated that equilibrium was reached within 120 min, and rapid separation was achieved within 30 s utilizing the superparamagnetic property. Mild elution was performed using an ethanol-formic acid (90:10, v/v) solution, achieving an OVA recovery rate exceeding 90 %. Selectivity experiments confirmed that the material exhibited significantly higher adsorption of glycoproteins (OVA, HRP) than non-glycoproteins (BSA, Lys). Practical application evaluation demonstrated efficient enrichment of trace glycoproteins from 500-fold diluted egg white and complex protein mixtures. SDS-PAGE analysis confirmed low non-specific adsorption, underscoring its strong potential for application in the pretreatment of complex biological samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Junzhu Li,Weiping Jin,Wei Xu,Gang Liu,Qingrong Huang,Zhenzhou Zhu,Shuyi Li,Shuiyuan Cheng
Similarities and differences of assembly for ovalbumin (OVA) and two kinds of sodium alginate (SA1 and SA2) varying in charge densities (λ SA1 : λ SA2 ≈ 2:1) were investigated. The assembly processes of OVA/SA mixtures were characterized by phase diagram, particle size, and microstructure. Two differences between OVA/SA1 and OVA/SA2 mixtures in the phase diagram were distinctly observed. First, due to the higher charge density of SA1, the strong interaction between OVA and SA1 caused only pH φ1 to be recorded. A higher linear charge density of SA1 narrowed the pH φ1 -pH φ2 range at ratios of 2:1 and 1:1. Second, OVA/SA1 complexes formed a coacervate with a relatively strong resistance to ion-induced shielding effects. This maintained the smaller size (tighter structure) with a larger number of complexes in the coacervate without 250 mM NaCl. The regulating polysaccharides with different charge densities could control the soluble region of complexes and endow various size or morphology of the coacervate assembled by proteins and polysaccharides.
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JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2020
·Vol. 72
·期 7
·956-968
·
IF 2.571
Wang Youpeng,Jing Weichao,Qu Wanying,Liu Zhiwei,Zhang Di,Qi Xiaoyu,Liu Lujia
ObjectivesThe aim of this study was to evaluate the effect of tectorigenin on treating allergic asthma model of guinea pigs and investigate the underlying mechanisms.MethodsAllergic asthma model of guinea pigs was established by sensitizing with ovalbumin (OVA). Then OVA-sensitized guinea pigs were injected with 10 mg/kg tectorigenin, 25 mg/kg tectorigenin or dexamethasone to investigate the effect of tectorigenin.Key findingsHigh dose of tectorigenin effectively decreased the number of coughs, the number of inflammatory cells and the levels of pro-inflammatory factors. Moreover, tectorigenin could inhibit pulmonary fibrosis in guinea pigs sensitized with OVA. In addition, the functions of tectorigenin were realized through downregulating profibrotic factors of transforming growth factor (TGF)-β1, phosphorylated (p)-Smad2/3 and Smad4, upregulating fibrosis-inhibitor of Smad7 and decreasing pro-inflammatory factors of vascular endothelial growth factor A (VEGFA), tumour necrosis factor-α (TNF-α), Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), p-inhibitor of nuclear factor-kappa B (NF-κB) kinase β (p-IKKβ) and NF-κB.ConclusionsTectorigenin could inhibit pulmonary fibrosis and airway inflammation through TGF-β1/Smad signalling pathway and TLR4/NF-κB signalling pathway. Therefore, tectorigenin might be a promising medicine to treat allergic asthma.
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BIOELECTROCHEMISTRY
·
2026
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IF 4.9
Luwen Ye,Yujia Yu,Daokun Xu,Zeng Feng,Liwei Mao,Lifeng Wang,Donglei Jiang
This study presents a novel sensing strategy for ovalbumin (OVA) by integrating a controllable molecularly imprinted polymer (MIP) fabrication technique with three-dimensional (3D) bioprinting technology. A MIP with high specificity and affinity was designed and synthesized for OVA using the reverse microemulsion epitope-directed surface imprinting and coating (ROSIC) method with silylating monomers. The synthesized MIP was then innovatively incorporated into gelatin methacryloyl (GelMA) and uesd as bioink to 3D bioprint a biomimetic small intestinal microtissue, which was subsequently assembled into a working electrode. Together with a homemade Ag/AgCl reference electrode and a platinum wire counter electrode, the resulting interface formed a three-electrode electrochemical sensing system for OVA detection. The constructed sensor exhibited a linear response to OVA concentration in the range of 10–400 pg/mL. The standard curve equation was R et = 11.12 C OVA (pg/mL) + 3261.9, with a correlation coefficient R 2 of 0.998, and a detection limit of 5.54 pg/mL.
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FOOD HYDROCOLLOIDS
·
2026
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IF 13.8
Shuhan Yong,Shanglin Li,Ting Zhang,Jingbo Liu,Zhiyang Du
Protein–polysaccharide hydrogels with clear gelation mechanisms and gastrointestinal robustness are attractive carriers for hydrophobic nutraceuticals. Here, a hydrogel composed of ovalbumin (OVA), konjac glucomannan (KGM), and genipin (GP), hereafter referred to as the OVA–KGM–GP hydrogel, was developed and found to form a self-supporting gel at room temperature. Apparent thermokinetic fitting provided an early clue that the OVA–KGM–GP system exhibited an integrated dehydration-stage signature rather than a simple additive thermal response, with ΔS ‡ values of −77.03 J mol −1 K −1 for OVA–KGM–GP and −88.55 J mol −1 K −1 for OVA–GP. Multiscale characterization further demonstrated that KGM guided OVA pre-assembly into interconnected structures, while GP fixed and compacted these structures into junction-like domains, yielding a hierarchically integrated lamellar–honeycomb network with elastic-dominant behavior. The hydrogel also exhibited enhanced gastric resistance, evidenced by a lower free amino group content after 2 h of simulated gastric digestion. As a delivery matrix, the hydrogel achieved a resveratrol encapsulation efficiency of 98.23%, and the bioaccessibility after 4 h simulated gastrointestinal digestion reached 70.23%, nearly two-fold that of free resveratrol (35.78%). These results support a bridging-locking assembly pathway for gelation and highlight its potential for hydrophobic nutraceutical delivery.
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TOXICOLOGY LETTERS
·
2025
·
IF 2.9
Xulong Cai,Qiaolan Xu,Tongjin Yin,Xia Li,Yan Cheng,Xiangning Li,Chuangli Hao
Allergens, a common trigger of asthma, can lead to increased levels of reactive oxygen species (ROS) and subsequent DNA damage. However, the repair mechanism of DNA damage caused by oxidative stress in allergen-induced asthma is less known. We explored the mechanism by which topoisomerase 1 binding arginine/serine rich protein (TOPORS) regulates small ubiquitin-related modifier 1 (SUMO1) modification of TOP1 to repair DNA damage induced by ovalbumin (OVA). We tested the expression level of TOP1 in asthmatic and healthy children. OVA-induced mouse asthma model was used for further validation. The level of SUMO1-TOP1 in macrophages was studied by immunoprecipitation. The expression of TOP1 was increased in asthmatic children. The expression of TOP1 and γ Histone 2AX (γH2AX) were increased in the lung tissue of asthmatic mice. In OVA-induced macrophages, the levels of TOP1 and γH2AX were increased, while knockdown expression of TOP1 could reduce the level of γH2AX. The level of SUMO1-TOP1 in OVA-induced macrophages was increased. Interestingly, knockdown expression of TOPORS could reduce the levels of SUMO1-TOP1 in OVA-induced macrophages, while the levels of TOP1 and γH2AX were increased. Our results indicate that TOPORS regulates SUMO1 modification of TOP1 and plays an important role in the repair of DNA damage induced by OVA. DNA repair in asthma exacerbation could be a therapeutic target.
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Innovative Food Science & Emerging Technologies
·
2026
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IF 6.9
Minghui Zhao,Jinghong Li,Sibing Li,Xiaohan Zhang,Hanyu Li,Huajiang Zhang
To mitigate post-harvest losses in field-grown fruits and thus contribute to global efforts against malnutrition and hunger, this study presents an edible coating inspired by the phase separation of ovalbumin (OVA) and chitosan (CS) complexes, resulting in distinct inner and outer functional layers. We demonstrate that the phase behavior of the OVA-CS complexes is modulated by resveratrol (RES). Specifically, RES modifies protein conformation and intermolecular interactions by enhancing hydrophobic forces and reorganizing hydrogen bonds, driving the self-assembly of a Janus-type coating during drying. Optimized at pH 5.6, the OVA-CS-RES (OCR) coating exhibits superior barrier properties, achieving the lowest water vapor permeability and oxygen permeability. Anisotropic wettability was confirmed by contact angle measurements and atomic force microscopy, with the maximum contact angle difference between the inner and outer surfaces reaching 57.2°. Scanning electron microscopy and confocal laser scanning microscopy further verified uniform coverage and effective sealing of epidermal pores on fruit surfaces. Application experiments showed that the OCR coating significantly delayed fruit oxidation, reduced weight loss, preserved texture and ascorbic acid content, and maintained aroma integrity throughout 40 days of storage. Overall, this study introduces a scalable, single-step coating strategy leveraging self-assembled functional Janus coatings to significantly extend fruit shelf life.
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