FOOD CONTROL
·
2020
·
IF 4.258
Qianchun Zhang,Yanqun Yang,Changbo Zhang,Yuguo Zheng,Yun Wu,Xingyi Wang
Patulin is a type of mycotoxin that can compromise both food quality and human health, and its potential toxic effects on human health are concerning. Therefore, strict monitoring of patulin content in food systems is necessary. However, discrimination and quantification of this toxin are difficult because of its extremely low concentration at pg/g levels in food samples. A highly specific sample preparation method is, thus, vital for patulin analysis. Herein, we present an innovative approach for the facile and controllable preparation of an aptamer-functionalized capillary monolithic polymer hybrid for the determination of patulin in food samples with high specificity and high affinity by using the aptamer-functionalized monolithic capillary polymer hybrid as a sorbent for solid-phase microextraction (SPME) coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The results confirmed that the developed SPME-UHPLC-MS/MS method was a sensitive and selective because of a low detection limit of 2.17 pmol/L and a linear range extending from 0.00811 to 8.11 nmol/L. Patulin was found to be present in apple juice samples (4.34 ng/kg). Furthermore, the recoveries were 85.4–106% with relative standard deviations ( n = 5) of 4.1–7.3%. Thus, the highly selective method will facilitate the ultrasensitive detection of target molecules in diverse applications.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Mengyang Xing,Yong Chen,Boqiang Li,Shiping Tian
Patulin is a toxic secondary metabolite produced by several moulds, which contaminates fruits and their products posing serious threats to human health. Though several microorganisms and enzymes have been reported to effectively degrade patulin, separation of them from fruit juice challenges the commercial applications. Here, a Pseudomonas aeruginosa strain TF-06 was isolated, its patulin degradation mechanism and optimum conditions for enzyme immobilization were investigated. The results indicated that TF-06 could degrade patulin into non-cytotoxic E/Z-ascladiol mainly by the activity of intracellular enzymes. For easy separation of enzymes, calcium alginate was selected for immobilization of intracellular enzymes from TF-06. The immobilized enzyme beads were effective in detoxification of patulin in apple juice. The mitigation rate was reached 95%, while there was no negative effect on juice quality. The study provides a promising way to resolve the issue of enzyme separation during mycotoxin biological detoxification in fruit juice.
研究领域:
食品科学
·
生物技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Jiadi Sun,Wei Guo,Jian Ji,Zaijun Li,Xiao Yuan,Fuwei Pi,Yinzhi Zhang,Xiulan Sun
As a kind of mycotoxins , patulin has been a usual contamination in apple juice and causes a serious public health concern nowadays. Regarding this issue, a novel and effective adsorbent, Fe 3 O 4 @SiO 2 @CS-GO@MIP, is designed to remove the patulin from apple juice. Firstly, the molecularly imprinted polymer (MIP) was prepared by surface imprinting technique. Meanwhile, addition of Fe 3 O 4 makes the final prepared MIP adsorbent magnetic, which is beneficial for separating the adsorbent from the food matrix. Moreover, chitosan (CS) and SiO 2 improve the biocompatibility, stability and dispersibility of the MIP adsorbent effectively. As for the adsorption performance, batches of experiments were conducted on the proposed adsorbent. Experimental results showed that the adsorption process followed pseudo-second-order kinetic and Freundlich isotherm model, and the physical multi-molecular layer endothermic adsorption happened during the whole adsorption process. Adsorption capacity of the proposed adsorbent is 7.11 mg/g maximumly, and over 90% of the total patulin in apple juice being removed after 24 h. Besides, the proposed adsorbent has distinguishing characteristics, including large specific surface area with BET reaching 279.6 m 2 /g, high cell viability of 79.77% and no negative effects on final quality parameters. The desirable adsorption performance and characteristics make Fe 3 O 4 @SiO 2 @CS-GO@MIP a promising adsorbent for removing the patulin from apple juice in the future.
研究领域:
食品安全
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Jingyimei Liang,Qian He,Yuning Zhao,Yahong Yuan,Zhouli Wang,Zhenpeng Gao,Zhongqiu Hu,Xubo Zhao,Tianli Yue
Patulin (PAT) is a common toxin in fruit juice, and adsorption is the most commonly used patulin removal method. The safety, adsorption capacity, and separation capacity of the adsorbent are the most important considerations. As a low-cost and biocompatible microbial material, bacterial cellulose gel membranes (BCMs) have many hydroxyl active sites that are readily modified, and BCMs are also stable, which facilitates their separation from aqueous solution. Therefore, BCMs are a promising material for adsorbing patulin. Through silanization, –SH was successfully connected to BCM, and the maximum content of –SH was 1.25 mmol/g. Because of facile combination of –SH and PAT, our sulfhydryl-modified bacterial cellulose gel membrane [BCM-SH(Si)] retained an adsorption capacity of 498.78 ± 35.94 μg/g in a complex apple juice solution, an adsorption capacity that is 125 × higher than that before the modification. We have thus demonstrated a promising, readily prepared biomaterial for removing of patulin from fruit juice that facilitates subsequent separation of the material from fruit juice and exhibits a good adsorption capacity.
研究领域:
食品科学
·
生物技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Baoshan He,Xiaoze Dong
In this work, the feasibility of a novel sensitive electrochemistry aptasensor for the detection of patulin (PAT) using hierarchically porous metal organic [email protected] blue connection aptamer as signal tags was demonstrated. The substrate electrode of the aptasensor was prepared by electrodeposited gold nanoparticles (AuNPs) on chitosan-zinc oxide nano flower-modified electrode. Subsequently, the complementary single-stranded DNA was attached to the surface of the modified electrode by Au-S bond. Signal tags self-assemble on the surface of modified electrodes by aptamer-cDNA binding, while methylene blue as mediator for electrochemical detection. Thanks to the large specific surface area provided by zinc oxide nano flower, the electrode can load more signal tags and AuNPs to achieve signal amplification. In presence of PAT, Apt preferentially binds to PAT, causing dissociation of some Apt-cDNA and releasing some signal tags leading, resulting in decreased electrical signals. Under optimal conditions, the change in electrochemical signal was proportional to the logarithm of PAT concentration, from 5 × 10 −8 -5 × 10 -1 μg mL −1 , and the detection limit was estimated to be 1.46 × 10 -8 μg mL -1 . The aptasensor exhibited aceptable reproducibility, renewability, long-term stability and specificity. Moreover, aptasensor was reliable in determination of PAT in spiked apple juice samples.
研究领域:
分析化学
·
纳米技术
·
电化学
·
食品安全
·
生物传感器
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Tianlin Wang,Meijun Lu,Yanbing Shen,Qinqing Han,Songming Wang,Tongyao Li,Tiange Li,Xianqing Huang,Libin Wan,Lianjun Song
Excessive levels of penicillin (PAT) in fruit juice pose significant risks to food safety and human health. In this study, a ratiometric fluorescence sensor with on-site visualization detection capability had been developed for PAT detection based on Förster resonance energy transfer (FRET) in this study. This strategy utilized MIL-101(Cr) with a wide absorption peak as the energy acceptor, while nitrogen/sulfur-doped graphene quantum dots (N,S-GQDs) conjugated with PAT aptamer with stable blue fluorescence serves as the energy donor, triggering the FRET effect and quenching the blue fluorescence. When exposed to PAT, the aptamer preferentially binded to PAT, separating the donor and acceptor, thereby interrupting the FRET effect and recovering the blue fluorescence. Assisted by the red internal reference fluorescence of gold clusters (AuNCs), the fluorescence changes were observed from red to blue with the concentration range of 0.01–300 ng/mL of PAT and a low limit of detection at 3.29 pg/mL. More importantly, a portable detection device integrated with smartphone was designed for on-site visual PAT detection with satisfactory results. This strategy could provide new opportunities for on-site qualitative and quantitative detection of PAT in actual foods.
研究领域:
食品安全
·
分析化学
·
生物传感器
·
纳米技术
·
荧光光谱学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xiaohai Yan,Jia Li,Fudai Cai,Xuejun Zeng,Sijia Li,Kai Wang,Qinglin Sheng,Kewei Feng,Yahong Yuan,Tianli Yue
Patulin (PAT) is a mycotoxin with immunotoxicity, genotoxicity, and neurotoxicity, so effective methods are needed for its detection. Fluorescence aptasensors provide a homogeneous detection method, but their sensitivity needs to be further improved. Herein, we developed a fluorescence aptasensor based on a target-oligonucleotide-driven DNAzyme motor and defective Zr-TCPE for PAT detection. The defective Zr-TCPE achieved an enhanced fluorescence intensity (increased by ∼1.9-fold) via defect engineering. The target-oligonucleotide-driven DNAzyme motor achieved effective PAT transduction, with a signal background ratio 4.4-fold that of the competitive transduction strategy. By combining defective Zr-TCPE with a target-oligonucleotide-driven DNAzyme motor, we achieved ultrasensitive detection of PAT with a detection limit as low as 9.38 fg/mL. In addition, the proposed fluorescence aptasensor could be used for the detection of PAT in apple juice, with recoveries ranging from 95.93% to 105.71%. This fluorescence aptasensor provides reasonable guidance for trace detection of hazardous substances in food and the environment.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2025
·
IF 2.2
Shumin Sun,Mengdan Ku,Jiayi Yang,Wenhao Zhang,Yanli Xie
A titanium dioxide/graphitic carbon nitride composite (TiO2/g-C3N4; TOCN) was utilized for the degradation of patulin (PAT). The introduction of carbon nitride into TOCN narrowed the band gap of titanium dioxide (TiO2), thereby enhancing the utilization of the composite with visible light. Patulin at a concentration of 250 μg/kg in aqueous solution and apple juice was degraded respectively by 100% and 85.4% under visible light. The superoxide ion (·O2-) and hydroxyl radical (·OH) play significant roles in the photocatalytic degradation of PAT, and a new degradation product was identified by ultra-high performance liquid chromatography tandem quadrupole orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). In addition, photocatalytic degradation has a minor impact on the quality and nutrition of apple juice. The results of Ames and zebrafish experiments demonstrated that the toxicities of the degradation products were significantly reduced. These results indicate the TOCN photocatalyst can effectively eliminate patulin from aqueous solutions and apple juice, offering a new strategy for removing patulin from food samples.
研究领域:
食品安全
·
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Caiping Huang,Bangzhu Peng
A high-performance nitrogen-doped chitosan-TiO2 nanocomposite to be used as a photocatalyst for patulin degradation in apple juice was successfully prepared by a simple precipitation method. The structure and properties were characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis diffuse reflectance spectrum. According to XPS and UV–Vis analyses, nitrogen had been successfully doped into the TiO2 nanoparticle crystals and reduced the band gap energy of TiO2 nanoparticles. The synthesized nanocomposite was able to photocatalytically degrade the patulin in apple juice under ultraviolet irradiation. The patulin (500 μg/kg) was completely degraded within 35 min, which exhibited significantly enhanced photocatalytic activities compared with TiO2 nanoparticles or chitosan-TiO2 nanocomposite. Moreover, the photocatalytic treatment significantly reduced the occurrence of juice browning, and had little effects on reducing sugar, pH, titratable acid and soluble solids of the apple juice. The nitrogen-doped chitosan-TiO2 nanocomposite would be an ideal photocatalyst for patulin degradation in apple juice due to its high photocatalytic activity and little effects on the quality of apple juice.
研究领域:
食品科学
·
环境科学
✓ 已验证
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 7
·1648-1657
·
IF 2.413
Wu Long,Yan Heng,Li Guanghui,Xu Xin,Zhu Lin,Chen Xiaoqiang,Wang Jing
As patulin is a kind of mycotoxin commonly found in fruit products, it is of great importance to develop simple and accurate analytical methods for the sensitive detection of patulin (PAT). In this work, based on molecular imprinted polymer (MIP) and surface-enhanced Raman scattering (SERS) technique, a specific and sensitive PAT sensor was developed. To prepare the MIP-SERS sensor, gold nanoparticles (Au NPs) were synthesized to act as supported substrate, 4-vinylpyridine (4-VP) served as functional monomer, 1,4-diacryloylpiperazine (PDA) served as cross-linker, PAT served as template molecule, and horseradish peroxidase (HRP) served as initiator. Owing to the good SERS character of Au NPs and excellent selectivity of MIP, the MIP-SERS sensor had a superior performance in PAT detection. Under the optimized conditions, this sensor exhibited a good linearity ( R 2 = 0.9877) with a range from 7.00 × 10 −12 to 5.00 × 10 −8 M for PAT. By simply incubating analytes with MIP-SERS substrate, the proposed method showed an enhanced SERS signal with a limit of detection of 5.37 × 10 −12 M ( S / N = 3). Besides, in the presence of interferences such as 2-oxindole (OXD) and 5-hydroxymethylfurfural (5-HMF), the MIP-SERS substrate showed good selectivity for PAT. What is more, the method had acceptable precision with recoveries of 96~108% in fruit product inspection, revealing its potential application in food analysis and safety verification.
.
研究领域:
分析化学
·
食品科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Shangyun Lu,Shuo Liu,Jinling Cui,Xiaoyi Liu,Chong Zhao,Lihong Fan,Shutao Yin,Hongbo Hu
Patulin (PAT) is the most common food-borne mycotoxin found in fruits and fruit-derived products, while chlorpyrifos (CPF) is a widely used pesticide on fruit and other crops. On the basis of the residue data, certain types of fruits can be contaminated simultaneously by patulin and chlorpyrifos. However, there are no available data about the combined toxicity. Since liver is a possible toxic target of both patulin and chlorpyrifos, we tested whether the combination exposure can cause enhanced hepatotoxicity using both cell culture and animal models. Results showed that the combination resulted in synergistic cytotoxicity in vitro and significantly enhanced liver toxicity in vivo. Mechanistically, PAT inhibited catalase activity via PIG3 induction, while CPF decreased catalase expression. These two mechanisms were converged in response to the combination, leading to enhanced inactivating catalase and boosted reactive oxygen species generation. The finding implicated that it is necessary to consider the combined toxicity in safety assessment of these food-borne contaminants.
研究领域:
毒理学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yue Qiu,Jiaping Yan,Xiaoling Liu,Yuting Pang,Yuting Ding,Fei Lyu
Patulin (PAT) is a hazardous mycotoxin frequently occurs in fruit industry. A reusable g-C 3 N 4 -SH@KG composite aerogel for PAT removal in a novel “dark adsorption-light regeneration” mode was prepared by thiol(-SH) functionalization and konjac glucomannan (KG) immobilization. The g-C 3 N 4 -SH@KG was characterized by SEM, FT-IR, XPS and UV–Vis DRS, and its PAT adsorption and photocatalytic regeneration behaviors and mechanisms were investigated. The g-C 3 N 4 -SH@KG exhibited good regeneration performance, maintaining 83% of PAT initial adsorption capacity (0.92 mg/g) after 5 “adsorption-regeneration” cycles. The adsorption process was endothermic and spontaneous. •OH and h + generated by photocatalysis were the main substances that degraded PAT into two products and regenerated -SH. The g-C 3 N 4 -SH@KG could effectively remove PAT without negative impact on juice quality. The study provided a new strategy for the regeneration of thiol-functionalized PAT adsorbents, and a new idea for the application of non-selective photocatalysis in the control of food contaminations.
研究领域:
食品科学
·
环境工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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