FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Shutong Wang,Changyong Cai,Xuhong Guo,Wang Yu,Zhen Zhang,Zhijian Tan
Due to their unique properties, holocellulose nanofibrils (HCNFs) are drawing more and more attention. Residual pectin and lignin may have significant impacts on the redispersion of HCNFs, but relevant studies are lacking. Herein, HCNFs with different residues were prepared from kenaf fibers using careful chemical pretreatments followed by mechanical disintegration. The chemical structure, crystalline phases, morphological features, redispersion properties and intermolecular interactions of different HCNFs were carefully characterized for clarifying the effects of residual pectin and lignin on the redispersion of HCNFs. Residual pectin significantly increased the water redispersibility of HCNFs with an aspect ratio of 241, whereas residual lignin decreased the water redispersibility of HCNFs with an aspect ratio of 48. After 30 days of storage, redispersed pectin-containing HCNFs (P-HCNFs) presented dispersion stability close to never-dried P-HCNFs. The possible mechanism for high redispersibility of P-HCNFs is the formation of synergistic electrostatic and steric repulsion forces between cellulose fibrils, triggered by carboxyl groups in residual pectin and large aspect ratio HCNFs. This study proposes a redispersion strategy for HCNFs isolated from kenaf fibers.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yanyan Qiao,Yixiao Shen,Hongzhou Jiang,Dongnan Li,Bin Li
In this study, three pectin polysaccharides BP1, BP2 and BP3, were purified from blueberries. The weight-average molecular weight (Mw) of BP1, BP2, and BP3 were detected to be 9.027 × 10 4 , 9.313 × 10 4 , and 1.223 × 10 6 Da, respectively. The structures of the three pectin polysaccharides were characterized and compared based on the results of molecular weight, monosaccharide composition, GC–MS and NMR analysis. Structural characterization revealed that BP1, BP2, and BP3 all contain homogalacturonan (HG) and rhamnogalacturonan I (RG-I) domains, and the rhamnose residues in RG-I domains are substituted at C-4 with side chains such as araban and galactosan. BP2 had the highest degree of esterification and HG domain ratio, followed by BP3 and BP1. In addition, BP1, BP2 and BP3 showed great antioxidant and antibacterial activities, and could destroy the cell membrane of Staphylococcus aureus and Escherichia coli . Moreover, the better DPPH and ABTS free radical scavenging and antibacterial activities of BP1 and BP2 than BP3 might be related to their lower molecular weight. The results of this study will provide essential information for the structure-activity relationship of pectin polysaccharides and research basis for development and application of blueberry pectin polysaccharides.
研究领域:
食品科学
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生物化学
·
药理学
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DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2023
·Vol. 39
·期 11
·1-17
·
IF 4.1
Song Xiaohui,Li Tang,Gu Hui,Yin Heng
Aspergillus is a well-studied fungal genus that is widely used in the processing of plant biomass in industries. This study investigated the effects of space exposure on the ability of Aspergillus costaricaensis , a filamentous fungus isolated from rotten orange peel, to degrade pectin. These fungal spores were carried into space by the Long March 5B carrier rocket and exposed to cosmic radiation for 79 h. After the flight, these spores were resuscitated, and then the growing strains were screened with pectin as the sole carbon source, and the pectinase activity was evaluated. A mutant with increased biomass accumulation ability and pectin-degrading activity compared to the ground control strain was obtained. Comparative transcriptome analysis revealed that several CAZymes genes were significantly upregulated in the mutant, especially those related to pectin degradation. Among the 44 pectinases identified from the annotated genome, 42 were up-regulated. The activities of these pectinases are able to synergistically break down the structure of pectin. In addition, the expression of some genes involved in metabolism, sugar transport, and stress response was altered. These results imply that space exposure might serve as a potential mutagenesis breeding technique, offering the opportunity to acquire biomass-degrading microbial strains with potential for industrial application.
研究领域:
微生物学
·
分子生物学
·
生物技术
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DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2016
·Vol. 41
·期 2
·e12860
·
IF 0.894
Wen-Xi Yu,Chang-Ying Hu,Zhi-Wei Wang
The aim of this study was to evaluate the kinetics of potassium sorbate (PS) loaded in low methoxyl pectin/carboxymethyl cellulose (PS-LMP/CMC) films into 95% ethanol. The ratio of LMP and CMC was changed from 8:2 to 0:10 by modulating the composition of the initial casting solution, and the corresponding diffusion coefficients were obtained from 0.26 × 10 −12 to 2.11 × 10 −12 cm 2 /s and showed a good linear relationship with the ratio of CMC in LMP/CMC films. The diffusivity and partition coefficient were increased with the increasing of CMC content. A decrease in temperature from 60C to 4C resulted in a reduction of diffusion coefficients from 3.11 × 10 −12 to 0.93 × 10 −12 cm 2 /s. The activation energy for the diffusion of PS in LMP/CMC (4:6) film was 16.42 kJ/mol. The release experiment at 15C showed that it is reliable to predicted release profile of PS from LMP/CMC (4:6) film according to the Fickian model. Practical Application Considered as one of the safest food preservatives, potassium sorbate (PS) is well known for its potential antimicrobial activity and widely used as preservatives in food products. As an environmental-friendly packaging material, low methoxyl pectin/carboxymethyl cellulose (LMP/CMC) antimicrobial films with PS can release antimicrobial agent onto food surface and enhance food safety, and could replace partially conventional plastics packaging material. The present study evidenced that the PS-LMP/CMC antimicrobial films control the release of PS by changing the ratio of polymer matrix. Compared to the experiments, the use of release models to evaluate the release profile of PS in antimicrobial films is economical and time saving and helpful to design packages with desired release of PS.
研究领域:
食品科学
·
高分子科学
·
包装技术
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控释材料
·
抗菌材料
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DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 11
·902
·
IF 5.3
Xi Zheng,Xin Feng,Yue Huang,Tao Zeng
In this study, the complexes (FG-P) based on fish gelatin (FG) and pectin (P) were prepared by a simple physical blending within a range of pectin concentrations (0–2%,w/v). The structure, interface, and emulsification properties of the obtained FG-P were analyzed. The binding between FG and pectin was dominated by electrostatic interaction and hydrogen bonding. Introducing pectin substantially increased the viscosity of FG-P. The water contact angle of FG-P gradually decreased with increasing pectin concentration. The highly interfacial viscosity and hydrophilicity of FG-P hindered the interfacial adsorption at the oil/water phase, thereby increasing the interfacial tension and phase angle. This was further manifested as an increase in the viscous modulus and a decrease in both the total modulus and elastic modulus. Despite the inhibition of interfacial adsorption, the unabsorbed FG-P was uniformly dispersed in the continuous phase to form a compact network structure, accompanied with improved rheological properties. Correspondingly, the emulsion precipitation phenomenon was effectively inhibited, and the stability of FG-P stabilized emulsions was improved with decreased droplet size.
研究领域:
食品科学
·
胶体与界面化学
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高分子科学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yinchun Liu,Fang Liu,Jianrui Liu,Jingwen Dong,Mingxia Xing,Xiangyu Chen,Ya'nan Wu,Tianxi Ai,Yongqin Zhang
Pectin depolymerase is widely utilized in various industrial sectors. However, the traditional methods for determining its enzymatic activity have limitations, such as cumbersome operations and a significant impact of enzyme solution dilution ratios on activity. The 3-methyl-2-benzothiazolinone hydrazone (MBTH) method can be employed to address these issues, but pectin precipitation and strong background commonly arise in this method. We have successfully overcome these challenges by employing a low-temperature and high-alkaline environment, and further optimized the reagent compositions and detection wavelength to improve the method. Consequently, enzyme hydrolysis follows a zero-order reaction within 60 min, which is helpful for the endpoint measurement of pectinase activity. The developed calibration curve for pectinase concentration and hydrolysis rate demonstrates linearity (R 2 = 0.9945) within the range of 2.5–15.8 mU/mL of pectinase. This method exhibits high sensitivity, accuracy, and stability, making it suitable for routine determination of pectin depolymerase activity in research and applications.
研究领域:
生物化学
·
工业酶学
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PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 8
·2152
·
IF 4.412
Qingtong Xie,Xudong Zheng,Liuting Li,Liqun Ma,Qihui Zhao,Shiyuan Chang,Lijun You
A pectin/chitosan matrix-loaded curcumin film (PCCF) with a deep eutectic solvent (DES) as the solvent and plasticizer was prepared in this study. Different quantities of curcumin (identified as PCCF-0, PCCF-1, PCCF-2. PCCF-3) were loaded on the pectin/chitosan film in order to evaluate their effects on the film properties. Results showed that curcumin could interact with the pectin/chitosan matrix and form a complex three-dimensional network structure. PCCF could promote the thickness, tensile strength, thermal properties, antioxidant and antiseptic capacities, but deteriorate the light transmission and elongation at the same time. The addition of curcumin would change the color of the film, without significantly affecting the moisture content. The tensile strength of PCCF-3 reached the maximum value of 3.75 MPa, while the elongation decreased to 10%. Meanwhile, the water-resistance properties of PCCF-3 were significantly promoted by 8.6% compared with that of PCCF-0. Furthermore, PCCF showed remarkable sustained antioxidant activities in a dose-dependent manner. PCCF-3 could inhibit DPPH and ABTS free radicals by 58.66% and 29.07%, respectively. It also showed antiseptic capacity on fresh pork during storage. Therefore, curcumin addition could improve the barrier, mechanical, antioxidant and antiseptic properties of the polysaccharide-based film and PCCF has the potential to be used as a new kind of food packaging material in the food industry.Keywords:chitosan;pectin;curcumin;film;DES
研究领域:
材料科学
·
食品科学
·
生物技术
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 56
·期 10
·5220-5229
·
IF 3.713
Ying-Xing Huang,Jun-Ru Qi,Jin-song Liao,Wen-Xin Jiang,Yong Cao,Jie Xiao,Xiao-Quan Yang
Summary The objective of this work was to extract high methoxyl (HM) pectin with oxalic acid, characterise its physicochemical properties and evaluate its dispersion stability in acidified milk drink (AMD). HPSEC–MALLS analysis revealed that the obtained HM pectin was high in molecular weight (522.4 kDa). The ζ-potential of the obtained HM pectin in aqueous solution at pH 4.0 was −31.5 mV. The AFM images proved that the obtained HM pectin contained several long linear molecules. The apparent diameter of AMD stabilised by the obtained HM pectin was 636 nm at the concentration of 3.0‰. The stability analysis of AMD and the microscopic observation with confocal laser scanning microscopy (CLSM) proved that the obtained HM pectin was a prominent stabiliser in AMD.
研究领域:
食品科学
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化学工程
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高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2019
·Vol. 36
·期 5
·822-827
·
IF 2.476
Pan Jie,Cao Duanlin,Ma Xuemei,Yang Jing
Curcumin is a kind of natural polyphenol whose functions are anti-tumor, anti-oxidant, anti-inflammatory and anti-microbial with low side effects. Solubility of Cur in water is poor, which results in low bioavailability, rapid metabolism, instability and poor absorption in vivo. Based on the crosslinking mechanism of pectin-calcium, the curcumin-pectin film (PT-Curf) was prepared by casting method, where chitosan and sodium alginate were added to the film, forming a system for blending modification to improve the performance of PT-Curf. Furthermore, the release of the curcumin films in vitro was studied and the effects of natural polysaccharides on the drug delivery system were discussed. According to our results, the natural polysaccharides involving pectin, chitosan and sodium alginate were excellent carriers of the fat-soluble drug Cur, and Cur was uniformly dispersed in the composite films in different crystal forms. Out of different drug delivery media, the three films exhibited different release rate and sustained release effect in vitro. However, the pectin-sodium alginate curcumin film (PT-SA-Curf) exhibited the most sustained release effect, the pectin-chitosan curcumin film (PT-CS-Curf) was second.
研究领域:
药学
·
材料科学
·
生物技术
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Huipeng Liu,Xiaoyu Wei,Shuyu Zu,Xihuang Lin,Jinchuang Zhang,Aimin Shi,Qiang Wang,Ning He
Plant-derived neutral oligosaccharides and pectic-oligosaccharides (POS) have been suggested as prebiotics based on their degree of polymerization (DP) and structural complexity. In the present study, neutral oligosaccharides without galacturonic acid were produced from commercial apple pectin using an optimized concentration of pectinase and degradation time. The crude oligosaccharide solution was refined by membrane separation using a DEAE-Sepharose CL-6B column, a graphite carbon column and an Xbridge C18 column, which resulted in the neutral oligosaccharides with DP 3–13. The monosaccharide composition of oligosaccharides primarily included glucose, arabinose, galactose and xylose without galacturonic acid. The structure of the oligosaccharides was analyzed using mass spectrometry with an electrospray ionization source by UPLC on an HILIC column and 1 H NMR. The MS/MS spectra in positive ion mode and 1 H NMR showed a main oligosaccharide structure corresponding to the connection of arabinogalactan with Galβ1→4Galβ1→4Galβ1→4Galβ1→α3Ara f . The growth of the selected Lactobacillus . strains was improved by adding refined neutral oligosaccharides with DP = 3–13 compared with commercial inulin , and growth of Escherichia coli had not basically grown. Therefore, neutral oligosaccharides (DP = 3–13) can be considered effective prebiotics for potential application in functional food.
研究领域:
食品科学
·
生物化学
·
微生物学
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高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Xiuxiu Yin,Yuxue Zheng,Xiangli Kong,Shuyun Cao,Shiguo Chen,Donghong Liu,Xingqian Ye,Jinhu Tian
The effects RG-І pectin (0.5, 1, 2, 5 and 10%, w/w) on the gelatinization behaviour, gel structure and digestibility of potato starch were characterized by means of rapid viscosity analysis (RVA), rheometry, scanning electron microscopy (SEM) and in vitro digestion. Results indicated that the addition of RG-I pectin significantly decreased the peak and breakdown viscosity and prolonged the pasting temperature of potato starch, but increased the setback viscosity. Meanwhile, the swelling power and amylose leaching of starch granules were restrained because of RG-I pectin adsorption on the starch granules surface. The presence of RG-I pectin also decreased the G’ values of potato starch gel but increased the tan δ values (especially for 1%), indicating that RG-І pectin decreased the 3D network formation of potato starch gel. The microstructure analysis indicated that RG-I pectin decreased the formation of the gel structure, which might place constraints on the relative amylose concentration in the continuous phase. The addition of RG-I pectin also reduced the rapidly digestible starch (RDS) content and increased the slowly digestible starch (SDS) content, while no significant differences of resistant starch (RS) content was observed. Those results indicated that RG-I pectin could affect the physicochemical properties of potato starch and modulate its digestibility and might be a potential option for producing starchy food with lower digestibility.
研究领域:
食品科学
·
化学工程
·
营养学
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DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2024
·
IF 2.4
Feng Li,Guohua Rao
Fig fruit is widely accepted in warm and tropical regions due to the sweet taste and health benefits. The polysaccharides in fig fruit are responsible for the health benefits. However, information regarding polysaccharide structure remains limited. In this study, the water-soluble polysaccharides (FFP) were extracted from fig fruit with a yield of 15.3 mg/g. Anion exchange chromatography and gel permeation chromatography were used for purification. A leading polysaccharide was purified and characterized as below. The monosaccharide composition of FFP included arabinose (Ara), galactose (Gal), galacturonic acid (GalA) and rhamnose (Rha). The glycosidic linkages were revealed to be L-Ara-(1→, →5)-L-Ara-(1→, →2,4)-L-Rha-(1→, →2)-L-Rha-(1→, →4)-D-Gal-(1→ and D-Gal-(1 → . Nuclear magnetic resonnance (NMR) spectra revealed that FFP had →4)-α-D-Gal p A-(1 → 2)-α-L-Rha p -(1 → as backbone. The side chains included Gal p -β-D-(1 → 4)-Gal p -β-D-(1 → 4)-Gal p -β-D-(1 → 4)-Gal p -β-D-(1→ and α-L-Ara f -(1 → 5)-α-L-Ara f -(1 → . They were linked to C-4 of Rha p . FFP inhibited the production of NO, IL-6 and IL-1β levels induced by LPS. IL-6 and IL-1β levels were returned to normal. These information are helpful to understand this functional fruit.
研究领域:
食品科学
·
药理学
·
生物化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）